CN113913359A - Solid culture medium for promoting growth of antrodia cinnamomea mycelia and synthesis of triterpenes - Google Patents
Solid culture medium for promoting growth of antrodia cinnamomea mycelia and synthesis of triterpenes Download PDFInfo
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- 241001486992 Taiwanofungus camphoratus Species 0.000 title claims abstract description 52
- 239000001963 growth medium Substances 0.000 title claims abstract description 38
- 150000003648 triterpenes Chemical class 0.000 title claims abstract description 30
- 239000007787 solid Substances 0.000 title claims abstract description 25
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 18
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 18
- 230000001737 promoting effect Effects 0.000 title claims abstract description 17
- 241000123852 Clinacanthus nutans Species 0.000 claims abstract description 37
- 238000002386 leaching Methods 0.000 claims abstract description 13
- 239000000284 extract Substances 0.000 claims abstract description 11
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims abstract description 9
- 239000001888 Peptone Substances 0.000 claims abstract description 9
- 108010080698 Peptones Proteins 0.000 claims abstract description 9
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 9
- 239000008103 glucose Substances 0.000 claims abstract description 9
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims abstract description 9
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims abstract description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 9
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 9
- 235000019319 peptone Nutrition 0.000 claims abstract description 9
- 239000012138 yeast extract Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 241000755731 Clintonia Species 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 6
- 241000607479 Yersinia pestis Species 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 4
- 229930003270 Vitamin B Natural products 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 239000011720 vitamin B Substances 0.000 claims description 3
- 235000019156 vitamin B Nutrition 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- -1 triterpene compound Chemical class 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 11
- 239000002994 raw material Substances 0.000 abstract description 6
- 239000011691 vitamin B1 Substances 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 abstract description 3
- 230000035784 germination Effects 0.000 abstract description 2
- 241000386927 Cinnamomum micranthum f. kanehirae Species 0.000 description 5
- 238000012258 culturing Methods 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 238000012364 cultivation method Methods 0.000 description 3
- 241000123370 Antrodia Species 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 241000133632 Aspergillus nutans Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 240000004784 Cymbopogon citratus Species 0.000 description 1
- 235000017897 Cymbopogon citratus Nutrition 0.000 description 1
- 244000166675 Cymbopogon nardus Species 0.000 description 1
- 235000018791 Cymbopogon nardus Nutrition 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000021438 curry Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
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- 239000002304 perfume Substances 0.000 description 1
- 239000010979 ruby Substances 0.000 description 1
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- 239000000344 soap Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
Abstract
The invention discloses a solid culture medium capable of promoting growth of antrodia cinnamomea mycelia and synthesis of triterpenes, and belongs to the field of biotechnology. The solid culture medium contains glucose, peptone, maltose, yeast extract, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, and vitamin B1And a Clinacanthus nutans leaching solution or a Clinacanthus nutans extract. The method has the characteristics that the antrodia cinnamomea mycelium has the rapid germination and growth, the triterpene content is 23.6 percent higher than that of the traditional culture medium, clinacanthus nutans can be easily obtained in China, the method is simple to prepare and high in repeatability, different types of culture media can be prepared according to actual production conditions by using the same raw material, and a large amount of antrodia cinnamomea mycelium can be provided for actual productionThe novel process of (1).
Description
Technical Field
The invention belongs to the field of biotechnology, and particularly relates to a solid culture medium capable of promoting growth of antrodia cinnamomea mycelia and synthesis of triterpenes.
Background
Antrodia camphorata (Antrodia camphorata)Antrodia cinnamomea) Is a rare medicinal fungus variety in Taiwan area of China, triterpenes are main functional components of the medicinal fungus, have a plurality of efficacies of protecting liver, detoxifying, resisting inflammation, resisting oxidation and the like, and research is carried out to the Cinnamomum kanehirae Hayata which is a two-stage Taiwan conservation tree species (Cinnamomum kanehirae Hayata, Cinnamomum kanehirae, and Cinnamomum kanehirae Hayae Hayata, and Cinnamomum kanehirae Hayata, which are secondary conservation trees in Taiwan to dateCinnamomum kanehirai) The Antrodia camphorata is the only host of Antrodia camphorata, and because the host is extremely small in the nature and the growth speed of the Antrodia camphorata is slow, the Antrodia camphorata is known as "ruby in forest". Clinacanthus nutans (A. nutans)Cymbopogon citratus(DC.) Stapf is a perennial dense-cluster aromatic herb of Gramineae and citronella, is widely planted in tropical regions, and is common in Guangdong, Fujian, Taiwan and the like in China. The stem and leaf can be used for extracting lemon essential oil for preparing perfume and soap, and can be eaten, and the tender stem and leaf is used as raw material for preparing curry flavoring; the medicine has the effects of dredging collaterals and dispelling pathogenic wind. Researches show that the mycelium of the antrodia camphorata can effectively replace fruiting bodies under partial conditions and has extremely similar health care functions, in order to solve the increasing demand of people on the antrodia camphorata, the method for screening the leaching solution of the clinacanthus nutans and the like as the culture medium can greatly and quickly promote the growth of the mycelium of the antrodia camphorata and the synthesis of the triterpene content, improve the content of effective components of the antrodia camphorata and simultaneously have correct guidance for the market development of the antrodia camphorataTherefore, the method can generate great economic and social benefits for the bacterial industry of China.
Disclosure of Invention
The invention aims to provide a solid culture medium for rapidly and efficiently promoting the growth of antrodia cinnamomea mycelia and the synthesis of triterpenes, and provides a scientific means for industrial production of antrodia cinnamomea mycelia and large accumulation of triterpenes.
In order to achieve the purpose, the invention adopts the following technical scheme:
a solid culture medium capable of promoting growth of antrodia camphorata mycelia and synthesis of triterpenes is prepared from glucose 2.5%, peptone 0.5%, maltose 0.3%, yeast extract 0.3%, potassium dihydrogen phosphate 0.1%, magnesium sulfate heptahydrate 0.1%, and vitamin B1Dissolving 0.1% and agar powder 1.5% in 1000 mL Clinacanthus nutans leaching solution, naturally adjusting pH, and sterilizing at 121 deg.C for 20 min.
The preparation method of the clinacanthus nutans leaching liquor comprises the following steps:
selecting fresh Clinacanthus nutans without plant diseases and insect pests, drying, crushing and screening by a 40-mesh sieve to obtain Clinacanthus nutans powder; decocting 50 g Clintonia nutans powder with 500-1000 mL of water at 70-90 deg.C for 30-60 min, filtering, and diluting the filtrate with water to 1000 mL to obtain Clintonia nutans leaching solution.
A solid culture medium capable of promoting growth of antrodia cinnamomea mycelia and synthesis of triterpenes is characterized in that: the formula of the culture medium comprises 2.5 percent of glucose, 0.5 percent of peptone, 0.3 percent of maltose, 0.3 percent of yeast extract, 0.1 percent of monopotassium phosphate, 0.1 percent of magnesium sulfate heptahydrate and vitamin B according to mass percentage1Dissolving 0.1% and 0.05% -0.25% of Clinacanthus nutans water extract in 1000 mL of water, sterilizing at 121 deg.C for 20 min under natural pH.
The preparation method of the clinacanthus nutans extract comprises the following steps:
selecting fresh Clinacanthus nutans without plant diseases and insect pests, crushing, drying, weighing and dissolving 20 g of Clinacanthus nutans in 1000 mL of water, heating in a water bath at 80-90 ℃, refluxing in vacuum for 3 h, filtering, concentrating the filtrate to 30 mL, freezing at-80 ℃, and drying in a vacuum drying oven or removing the solvent by adopting a spray drying mode to obtain a tan colloidal solid or a powdery solid, namely the Clinacanthus nutans water extract.
The solid culture medium is used for promoting the growth of antrodia camphorata mycelium and the synthesis of triterpene.
The method for culturing antrodia by the culture medium prepared by the invention comprises the following steps: culturing at 28 deg.C in dark for 14-28 days.
After the antrodia camphorata mycelia cultured by the prepared culture medium are inoculated and cultured for 2-3 days, the mycelia begin to germinate rapidly in large quantity, grow over all flat plates (the diameter is 10 cm) at 20-28 days, and the content of triterpenes in the antrodia camphorata mycelia can be up to 7.79 +/-0.13 mg/g measured by a vanillin-glacial acetic acid method.
The invention has the following remarkable advantages:
the antrodia camphorata is a rare medicinal fungus variety in Taiwan in China, and the artificial cultivation method comprises a basswood cultivation method, a solid cultivation method and a liquid fermentation method, but the method for cultivating the antrodia camphorata has high cost and long cultivation period, and the biomass of mycelium and the yield of intracellular triterpene obtained by cultivation are low. The Clinacanthus nutans is large in quantity, wide in source and low in price in China, and the Clinacanthus nutans leaching solution or Clinacanthus nutans extract is used for preparing the solid culture medium, so that germination and growth of Antrodia cinnamomea mycelia can be efficiently and quickly promoted, and the culture time is shortened by about 30% compared with that of a traditional culture medium; meanwhile, the solid culture medium prepared by the invention can also effectively promote the synthesis of antrodia camphorata triterpenes, so that the content of the triterpenes in the antrodia camphorata mycelia is as high as 7.79 +/-0.13 mg/g, which is 23.6% higher than that of the traditional culture medium. The facts show that the culture medium can replace the traditional culture medium, thereby solving the bottleneck in artificial production and cultivation of the antrodia camphorata and realizing the rapid industrialization of the antrodia camphorata mycelia.
Drawings
FIG. 1 shows the growth of Antrodia camphorata in example 1. In which, YDC is the solid medium for promoting the growth of antrodia cinnamomea mycelium and the synthesis of triterpene described in example 1, and CK is a control medium.
Detailed Description
In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.
Example 1
Selecting fresh Clinacanthus nutans without plant diseases and insect pests, drying, crushing and screening by a 40-mesh sieve to obtain Clinacanthus nutans powder; decocting 50 g Clintonia nutans powder with 500-1000 mL of water at 70-90 deg.C for 30-60 min, filtering, and diluting the filtrate with water to 1000 mL to obtain Clintonia nutans leaching solution. The method comprises the following raw materials in percentage by mass: 2.5% glucose, 0.5% peptone, 0.3% maltose, 0.3% yeast extract, 0.1% monopotassium phosphate, 0.1% magnesium sulfate heptahydrate, 0.1% vitamin B1And 1.5% agar powder, dissolving in 1000 mL clinacanthus nutans leaching liquor, naturally adjusting pH, and sterilizing at 121 ℃ for 20 min to obtain the solid culture medium capable of promoting growth of antrodia cinnamomea mycelia and synthesis of triterpenes.
The formula of the reference culture medium comprises the following raw materials in percentage by mass: 2.5% glucose, 0.5% peptone, 0.3% maltose, 0.3% yeast extract, 0.1% monopotassium phosphate, 0.1% magnesium sulfate heptahydrate, 0.1% vitamin B1And 1.5% agar powder in 1000 mL water, with natural pH, and sterilizing at 121 deg.C for 20 min.
After the culture medium is cooled, the antrodia camphorata strain is inoculated. The culture method comprises the following steps: culturing at 28 deg.C in dark for 16-28 days. The growth of Antrodia camphorata is shown in figure 1.
According to measurement, the growth rate of antrodia camphorata mycelium is 1.13 +/-0.04 mm/d and the content of triterpene is 6.30 +/-0.10 mg/g under the culture of a control culture medium; and when the culture medium containing the clinacanthus nutans leaching liquor is cultured, the growth rate of the antrodia camphorata mycelium is 1.39 +/-0.03 mm/d, the content of the triterpene is 7.74 +/-0.11 mg/g, and the growth rate of the mycelium and the content of the triterpene are remarkably different from those of a control group (P < 0.05).
Example 2
Selecting fresh Clinacanthus nutans without plant diseases and insect pests, breaking, crushing, drying, weighing and dissolving 20 g of Clinacanthus nutans in 1000 mL of water, heating in a water bath at 90 ℃, refluxing in vacuum for 3 h, filtering, concentrating the filtrate to 30 mL, freezing at-80 ℃, and putting into a vacuum drying oven to obtain a tan colloidal solid, namely the Clinacanthus nutans water extract. The method comprises the following raw materials in percentage by mass: 2.5% glucose, 0.5% peptone, 0.3% maltose, 0.3% yeastExtract, 0.1% potassium dihydrogen phosphate, 0.1% magnesium sulfate heptahydrate, and 0.1% vitamin B1And dissolving the clinacanthus nutans extract in 1000 mL of water, performing natural pH, and sterilizing at 121 ℃ for 20 min to obtain the solid culture medium capable of promoting growth of antrodia cinnamomea mycelia and synthesis of triterpenes. Wherein the Clinacanthus nutans extract is 0.05%, 0.1%, 0.15%, 0.2%, 0.25% by weight respectively.
The formula of the reference culture medium comprises the following raw materials in percentage by mass: 2.5% glucose, 0.5% peptone, 0.3% maltose, 0.3% yeast extract, 0.1% monopotassium phosphate, 0.1% magnesium sulfate heptahydrate, 0.1% vitamin B1And 1.5% agar powder in 1000 mL water, with natural pH, and sterilizing at 121 deg.C for 20 min.
After the culture medium is cooled, the antrodia camphorata strain is inoculated. The culture method comprises the following steps: culturing at 28 deg.C in dark for 16-28 days. The growth of Antrodia camphorata is shown in Table 1.
According to measurement, the growth rate of antrodia camphorata mycelium is 1.13 +/-0.04 mm/d and the content of triterpene is 6.3 +/-0.10 mg/g under the culture of a control culture medium; and when the culture medium containing 0.2% of Clinacanthus nutans aqueous extract is cultured, the growth rate of the mycelium of the antrodia camphorata is 1.43 +/-0.05 mm/d, the content of the triterpene is 7.79 +/-0.13 mg/g, and the contents of the mycelium of the antrodia camphorata and the triterpene are remarkably different from those of a control group (P < 0.05).
TABLE 1 growth rates of Antrodia camphorata mycelia
The facts show that the preparation of the solid culture mycelium of antrodia camphorata by using the Clinacanthus nutans water extract and the Clinacanthus nutans leaching solution can obviously shorten the culture time, improve the production efficiency in production and reduce the production cost.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (6)
1. A triterpene compound capable of promoting growth of Antrodia Camphorata myceliumThe solid culture medium is characterized in that: the formula of the culture medium comprises 2.5 percent of glucose, 0.5 percent of peptone, 0.3 percent of maltose, 0.3 percent of yeast extract, 0.1 percent of monopotassium phosphate, 0.1 percent of magnesium sulfate heptahydrate and vitamin B according to mass percentage1Dissolving 0.1% and agar powder 1.5% in 1000 mL Clinacanthus nutans leaching solution, naturally adjusting pH, and sterilizing at 121 deg.C for 20 min.
2. The solid culture medium for promoting the growth of antrodia camphorata mycelium and the synthesis of triterpenes according to claim 1, wherein: the preparation method of the clinacanthus nutans leaching liquor comprises the following steps:
selecting fresh Clinacanthus nutans without plant diseases and insect pests, drying, crushing and screening by a 40-mesh sieve to obtain Clinacanthus nutans powder; decocting 50 g Clintonia nutans powder with 500-1000 mL of water at 70-90 deg.C for 30-60 min, filtering, and diluting the filtrate with water to 1000 mL to obtain Clintonia nutans leaching solution.
3. A solid culture medium capable of promoting growth of antrodia cinnamomea mycelia and synthesis of triterpenes is characterized in that: the formula of the culture medium comprises 2.5 percent of glucose, 0.5 percent of peptone, 0.3 percent of maltose, 0.3 percent of yeast extract, 0.1 percent of monopotassium phosphate, 0.1 percent of magnesium sulfate heptahydrate and vitamin B according to mass percentage1 Dissolving 0.1% and 0.05% -0.25% of Clinacanthus nutans extract in 1000 mL of water, sterilizing at 121 deg.C for 20 min under natural pH.
4. The solid culture medium for promoting the growth of Antrodia camphorata mycelium and the synthesis of triterpene according to claim 3, wherein: the preparation method of the clinacanthus nutans extract comprises the following steps:
selecting fresh Clinacanthus nutans without plant diseases and insect pests, crushing, drying, weighing and dissolving 20 g of Clinacanthus nutans in 1000 mL of water with 600-.
5. The use of the solid culture medium according to claim 1 for promoting the growth of Antrodia camphorata mycelium and the synthesis of triterpene.
6. The use of the solid medium according to claim 3 for promoting the growth of Antrodia camphorata mycelium and the synthesis of triterpene.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104130948A (en) * | 2014-07-11 | 2014-11-05 | 福建农林大学 | Preparation method and application of Antrodia camphorate mycelium medium |
| CN105001009A (en) * | 2015-08-13 | 2015-10-28 | 福建农林大学 | Antrodia cinnamomea terpene-rich mycelium culture medium and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104130948A (en) * | 2014-07-11 | 2014-11-05 | 福建农林大学 | Preparation method and application of Antrodia camphorate mycelium medium |
| CN105001009A (en) * | 2015-08-13 | 2015-10-28 | 福建农林大学 | Antrodia cinnamomea terpene-rich mycelium culture medium and application |
Non-Patent Citations (2)
| Title |
|---|
| 于群: "忧遁草活性成分的提取及其功能评价", 于群, no. 2018, pages 024 - 599 * |
| 冯路瑶等: "不同中药提取物对牛樟芝生长和胞内三萜产物形成的影响", 中国食用菌, vol. 37, no. 2, pages 42 - 46 * |
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