CN105238703B - The dendritic cladosporium of one plant height production feruloyl esterase and the application in vinegar brewing - Google Patents
The dendritic cladosporium of one plant height production feruloyl esterase and the application in vinegar brewing Download PDFInfo
- Publication number
- CN105238703B CN105238703B CN201510705775.3A CN201510705775A CN105238703B CN 105238703 B CN105238703 B CN 105238703B CN 201510705775 A CN201510705775 A CN 201510705775A CN 105238703 B CN105238703 B CN 105238703B
- Authority
- CN
- China
- Prior art keywords
- vinegar
- fermentation
- application
- cladosporium
- feruloyl esterase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
Dendritic cladosporium the invention discloses plant height production feruloyl esterase and the application in vinegar brewing, belong to Fermentation Engineering and biological technical field.The dendritic cladosporium (Cladosporium cladosporioides) of the present invention, by China typical culture collection center preservation, deposit number is CCTCC NO：M 2015549, preservation date are September 16 in 2015.The present invention carrys out fermenting and producing high activity feruloyl esterase by fermented and cultured matrix of wheat bran, it is easy to accomplish the industrialized production of feruloyl esterase.And feruloyl esterase is applied in vinegar production, the functional health vinegar rich in forulic acid is obtained, new technical support is provided for the exploitation of health-care vinegar.
Description
Technical field
Dendritic cladosporium the present invention relates to plant height production feruloyl esterase and the application in vinegar brewing, belong to fermentation
Engineering and biological technical field.
Background technology
Feruloyl esterase (FEA, EC 3.1.1.73) is a subclass of carboxylic ester hydrolases, and primary biological function is water
Plant cell wall polysaccharides and the ester bond of forulic acid (FA) connection are solved, so as to which forulic acid and other cinnamic acids be released.Mesh
Before, report production feruloyl esterase bacterial strain there are about 30 plants, wherein mainly include aspergillus niger, aspergillus oryzae, Aspergillus terreus, aspergillus flavus,
Aspergillus flavipes, aspergillus awamori, Tabin aspergillus, aspergillus nidulans, Aspergillus usamii, head mold, mould, sporotrichum thermophile, sharp spore reaping hook
The basket bacterium of bacterium, fusarium prolifertum, Neuraspora crassa, rhizomucor miehei, handle, greening russule, Pleurotus eryngii, Aureobasidium pullulans, anaerobism
Fungi, new beautiful silk bacterium etc., but the vigor of these bacterial strains production feruloyl esterase is generally relatively low, is insufficient for commercial Application.
Also have through genetic modification means to improve the correlative study that microorganism produces feruloyl esterase ability, but the engineering built at present
Producing strain is not very high, and industrialization production requirements are still not achieved.Therefore the wild type ferulic acid ester that enzyme activity is high, safe is explored
Enzyme producing strains seem necessary, and there is greatly research and development to be worth.
Forulic acid because became the focus of people's research always in recent years with a variety of physiological active functions.It has reported at present
Road, forulic acid have hydrogen peroxide, superoxide radical, hydroxy radical, peroxynitrite the strong broken effect of going out.Forulic acid
The effect of reducing blood pressure can be played by the effect to Endothelin (Endothelin, ET).Forulic acid is to common cold virus, breathing
Road syncytial virus and AIDS virus significantly inhibit work.Forulic acid can reduce the oxygen demand of myocardial ischemia, clinically
Have been used for treatment coronary heart diseases and angina pectoris.In recent years, related ferulic acid and its derivatives inhibit colon and rectum carcinoma and tongue cancer
It is reported in and is continuously increased.And find that forulic acid has remarkable result in terms of protect liver, treatment diabetes.
At present, external being reported in for the functional food in relation to forulic acid is gradually increased, it is contemplated that utilizing microorganism
Biological waste resource is converted by resource by fermentation engineering to be added to using substance in Vinegar Fermentation system, is made one
Functional health vinegar of the kind rich in forulic acid, increases the exploitation of health-care vinegar.
The content of the invention
The technical problems to be solved by the invention are：It is relatively low for current microorganism ferulaic acid esterase activity, it can not realize
The problem of industrial applications, provides the dendritic cladosporium of one plant of new high yield feruloyl esterase, ferments using wheat bran
Feruloyl esterase is generated, is added to for the first time during Vinegar Fermentation, the obtained vinegar rich in forulic acid has anti-oxidant, drop blood
Pressure, antithrombotic, reducing blood lipid, antibacterial anti-inflammatory, pre- anti-cancer, protect liver, treatment diabetes and other effects.
First purpose of the present invention is to provide the dendritic cladosporium of one plant of new high yield feruloyl esterase, from Zhejiang
Ta Pai Shaoxing rice wines Co., Ltd yellow rice wine wheat starter by screening, isolates and purifies.Dendritic cladosporium (the Cladosporium
Cladosporioides), by China typical culture collection center preservation, abbreviation CCTCC, deposit number is CCTCC NO:M
2015549, preservation date September 16 in 2015, preservation address is Wuhan, China Wuhan University.
The tablet observation feature of the dendritic cladosporium of gained of the invention is as follows：72h, bacterial strain are cultivated on PDA solid mediums
Bacterium colony is thick, in heavy fleece hairy, is tightly combined with culture medium, is not easy to provoke.Color is just infuscate to green and brown color, and the later stage is dark brown
Color is to black.Bacterium colony back side black.
The rDNA ITS1-5.8S-ITS2 sequences of the dendritic cladosporium and Cladosporium cladosporioides
Strain FR5-SAR1 homogeneity reaches 100%.
Second object of the present invention is to provide the mould application in terms of feruloyl esterase is produced of the branch spore.
The production feruloyl esterase is the main component using wheat bran as culture medium.
The application is that dendritic cladosporium seed liquor is inoculated in wheat bran liquid state fermentation culture according to 3~8% inoculum concentration
It is cultivated, wheat bran dosage 0.5~6% (remaining is water), 32~35 DEG C, 48~120h of incubation time of cultivation temperature, is led in base
0.5~2vvm of oxygen amount, 100~150r/min of rotating speed, pH4.8~6.5.
NaCl 0.3g/L, (NH can also be contained in the wheat bran liquid state fermentation culture medium4)2SO41.3g/L, MgSO4·
7H2O0.3g/L, K2HPO40.3g/L。
Third object of the present invention is to provide application of the dendritic cladosporium in terms of vinegar brewing.
The application is in the dendritic cladosporium of wheat bran liquid state fermentation inoculation of medium, carries out liquid fermentation to obtain fermentation material
Liquid；Then fermented feed liquid is added to vinegar brewing Luo Gang workshop sections according to the amount for accounting for raw material glutinous rice quality 10~40%, while
Repeatedly stream adds the fermented feed liquid for accounting for Raw Materials Rice quality 3~12% for acetic fermentation workshop section.
The application is respectively to flow to add one time fermentation feed liquid in 72h, 96h, 120h of acetic fermentation.
The vinegar brewing includes rice steeping, steamed rice, drenches meal, falls cylinder, saccharification, alcoholic fermentation, acetic fermentation, leaching vinegar, sterilization
Workshop section.
Compared with prior art, the beneficial effects of the invention are as follows：
1. the present invention provides a kind of new dendritic cladosporium (Cladosporium cladosporioides), the bacterium
Strain has the ability of high yield feruloyl esterase, the data bank greatly supplemented with extraction bound form forulic acid bacterium；The dendritic branch
Spore bacterium isolates and purifies out from living nature, and is easy to culture and preserves, and breeding is rapid, and growth ability is strong；
2. the enzyme activity of the dendritic cladosporium fermentation production feruloyl esterase of the present invention can reach 308U/L, can be used as from plant
Extract the novel wild bacterium of bound form phenolic acid；
During 3. the present invention creatively produces dendritic cladosporium wheat bran fermented feed liquid first Application to vinegar, comprehensive utilization
By-product-wheat bran during wheat processing realizes exploitation high-quality vinegar and the double benefit of energy-saving and emission-reduction；Branch spore
The use of mould wheat bran fermented feed liquid on the premise of original vinegar flavor mouthfeel is ensured, has and is obviously improved ferulaic acid content
Effect.
4. by the vinegar that the production method of the present invention obtains, ferulaic acid content reaches 45~80ppm in final measure vinegar,
Bright color, alcoholic strength is low, and clean taste is unique, and with certain healthcare function.
Biological deposits information
Dendritic cladosporium, taxology are named as dendritic cladosporium S2Cladosporium cladosporioides S2, in
September in 2015 is preserved in China typical culture collection center on the 16th, and preservation address is Wuhan, China Wuhan University, deposit number
For CCTCC NO:M 2015549.
Description of the drawings
Fig. 1：Colonial morphology of the dendritic cladosporium on primary dcreening operation culture medium；
Fig. 2：Dendritic cladosporium PDA isolate and purify after colonial morphology；
Fig. 3：HPLC measures the chromatogram of dendritic cladosporium zymotic fluid.
Specific embodiment
Culture medium prescription：
Primary dcreening operation culture medium：NaCl 0.3g, (NH4)2SO41.3g, MgSO4·7H2O 0.3g, K2HPO40.3g, agar powder
18g, 15ml ferulic acid ethyl ester (are dissolved in 10%V/V dimethylformamides), and distilled water 1000ml, pH are naturally, 121 DEG C of sterilizings
20min。
Pure medium：Potato agar solid medium (PDA solid mediums), potato 200g, sucrose 20g, fine jade
Fat 18g, distilled water 1000ml, pH are naturally, 121 DEG C of sterilizing 20min.
Seed culture medium：Liquid PDA culture medium is not added with agar, and pH is naturally, 121 DEG C of sterilizing 20min.
Secondary screening culture medium：NaCl 0.3g, (NH4)2SO41.3g, MgSO4·7H2O 0.3g, K2HPO40.3g, distilled water
1L, pH 6.5.10g wheat bran is weighed in 250mL triangular flasks, adds 100mL liquid portions, sterilize 20min in 121 DEG C, obtains
Secondary screening culture medium.The seed liquor cultivated for 24 hours is inoculated into for 4% inoculum concentration in 100mL secondary screening culture mediums by volume, in 30
DEG C, cultivate certain time under 200r/min.
Embodiment 1：The screening of cladosporium sp strain
The primary dcreening operation of dendritic cladosporium possesses by using ferulic acid ethyl ester as sole carbon source, generating transparent circle phenomenon and filtering out
Produce the bacterial strain of feruloyl esterase ability.
Vinegar wheat koji sample is taken from Zhejiang Province Tapai Shaoxing Wine Co., Ltd, takes wheat koji 10g, is placed on containing the sterile steamings of 90ml
In the 250mL triangular flasks of distilled water and bead, sealing, 200r/min shakes 30min on shaker, makes wherein microorganism abundant
It is scattered, it is made 10-1Times sample diluting liquid.
On superclean bench 10 are obtained with gradient dilution method-2、10-3、10-4、10-5、10-6、10-7Times sample diluting liquid,
Take 10-4、10-5、10-6、10-7Four each 0.2mL of dilution gradient sample liquid are coated on primary dcreening operation culture medium, are placed in 28 DEG C of incubators
After cultivating 3d, transparent circle size is observed.
After primary dcreening operation culture, according to the size of periphery of bacterial colonies transparent circle on primary dcreening operation culture medium, (Fig. 1 is the dendritic branch spore of the present invention
Colonial morphology of the bacterium on primary dcreening operation culture medium), the big bacterium colony of picking transparent circle carries out gradient dilution, is coated on primary dcreening operation tablet,
Culture 2d in 28 DEG C of incubators, obtains the microorganism single bacterium colony of production feruloyl esterase.Single bacterium colony is taken to be inoculated in PDA culture medium, 28
Culture 3d, -80 DEG C of conservations of glycerine, 4 DEG C of inclined-plane conservation in DEG C incubator.
Primary dcreening operation inoculation by isolating and purifying is contained in secondary screening culture medium by measuring forulic acid in secondary screening culture medium
Amount height carries out secondary screening, obtains the bacterial strain of high yield feruloyl esterase, measures its enzyme activity as 308U/L.
Embodiment 2：The Microbiological Characteristics of bacterial strain and identification
The results are shown in Figure 2 after bacterial strain isolates and purifies on PDA plate, and bacterial strain bacterium colony is thick, in heavy fleece hairy, with culture
Base is tightly combined, and is not easy to provoke.Color is just infuscate to green and brown color, and the later stage is dark brown to black.Bacterium colony back side black.Just
Step is judged as mould.
Bacterial strain total DNA is extracted as template, PCR amplification rDNAITS1-5.8S-ITS2 areas, through objective gene sequence is sequenced
Length is 520bp, GenBank sequence numbers:KP900248.1.The rDNAITS1-5.8S-ITS2 sequences of bacterial strain and 10 plants of bacterial strains
Homogeneity (Identity) more than 90.0%, and with Cladosporium cladosporioides strain therein
FR5-SAR1 homogeneity reaches 100%, builds molecular evolutionary trees, it can be seen that bacterial strain S2 and Cladosporium
Cladosporioides is on the same branch of the chadogram of structure, with reference to the Morphological Identification and rDNAITS1-5.8S- of bacterial strain
ITS2 the sequencing results, it is dendritic cladosporium sp strain to judge bacterial strain of the present invention.
Embodiment 3：Application of the bacterial strain wheat bran zymotic fluid in vinegar production
Wheat bran 0.5kg, distilled water 10L, pH4.8, lead to oxygen 1vvm, rotating speed 150r/min, under the conditions of 28 DEG C of temperature, are sent out
Ferment takes the liquid fermentation liquid of fermentation 76h, utilizes high-efficient liquid phase color spectrometry ferulaic acid esterase activity (as shown in Figure 3), enzyme activity
For 280U/L.
Chromatographic column：XBridgeTM C18 (4.6 × 250mm, 5 μm)
Mobile phase：(B：0.01% glacial acetic acid aqueous solution；Mobile phase D：Pure acetonitrile).Flow velocity 1.0mL/min.
Gradient elution method：Initial time, B solution flow 80%, solution D flow 20% maintain 2min；8min, B solution
Flow 72%, solution D flow 28%；13min, B solution flow 42%, solution D flow 58%；19min, B solution flow 80%,
Solution D flow 20% maintains 6min, terminates.Obtained linearity curve is：Y=2.79 × 104X+6.65×103, R2=
0.9982.
Ferulaic acid esterase activity measures
Determination step：250 μ L fermentation crude enzyme liquids are taken, add in 250 μ L, pH 6.0Na2HPO4-C6H8O7Buffer preparation
Ferulic acid methylester solution adds in 500 μ L, 10% glacial acetic acids (V/V), 4 DEG C, 10000r/min centrifugations after reacting 10min at 50 DEG C
20min, crosses 0.22 μm of filter membrane, and HPLC methods measure ferulaic acid content.
Blank control replaces Ferulic acid methylester solution by 250 μ L of distilled water, and other reactions are identical with measure group.
Enzyme activity defines：Under the conditions of 50 DEG C, pH 6.0, esterlysis Ferulic acid methylester per minute is generated needed for 1 μm of ol forulic acid
Enzyme amount is defined as 1 enzyme activity unit (U).
Fermented feed liquid is added to vinegar brewing Luo Gang workshop sections according to 30% amount (percentage for accounting for raw material glutinous rice quality),
Simultaneously in acetic fermentation workshop section 72h, 96h, 120h, after afterflow plus the fermentation material of 5% (percentage for accounting for Raw Materials Rice quality)
Liquid.
Finally measuring ferulaic acid content in vinegar reaches 57ppm.
Embodiment 4：This product and the comparison of zhenjiang vinegar physical and chemical index
The functional vinegar and the physical and chemical index of zhenjiang vinegar that embodiment 3 is obtained, the results are shown in Table 1.Divided by table 1
Analysis understands that embodiment 3 produces the zhenjiang vinegar that vinegar belongs to total acid >=5.00g/L, and ferulaic acid content improves 9 times or more.
1 vinegar physical and chemical index of table compares
Embodiment 5：The influence of fermented feed liquid additive amount and addition time to finished product vinegar physical and chemical index
During fermented feed liquid is added to vinegar brewing according to Different adding amount and addition time, the finished product vinegar of acquisition
Physical and chemical index is to such as table 2.It is analyzed by table 2, according to the vinegar Ah of the method for the invention addition fermented feed liquid production
Wei's acid content reaches zhenjiang vinegar standard in more than 45ppm, and the other physical and chemical indexes of vinegar are good.
2 vinegar physical and chemical index of table compares
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not limited to the present invention, any to be familiar with this skill
The people of art without departing from the spirit and scope of the present invention, can do various change and modification, therefore the protection model of the present invention
Enclosing be subject to what claims were defined.
Claims (13)
- A kind of 1. dendritic cladosporium (Cladosporium cladosporioides), which is characterized in that the dendritic cladosporium China typical culture collection center is preserved within 16th in September in 2015, preservation address is Luojiashan, Wuchang, Wuhan City, Hubei Province Wuhan University, deposit number are CCTCC NO:M 2015549.
- 2. a kind of dendritic cladosporium according to claim 1, which is characterized in that the dendritic cladosporium screening is from yellow rice wine Wheat koji.
- 3. application of the dendritic cladosporium described in claim 1 in terms of feruloyl esterase is produced.
- 4. application according to claim 3, which is characterized in that the production feruloyl esterase is using wheat bran as culture medium Main component.
- 5. application according to claim 3, which is characterized in that the application be by dendritic cladosporium seed liquor according to 3~ 8% inoculum concentration is inoculated in wheat bran liquid state fermentation culture medium and is cultivated, wheat bran dosage 0.5~6%, cultivation temperature 32~35 DEG C, 48~120h of incubation time, 0.5~2vvm of oxygen-supply quantity, 100~150r/min of rotating speed, pH4.8~6.5.
- 6. application of the dendritic cladosporium in terms of vinegar brewing described in claim 1.
- 7. application according to claim 6, which is characterized in that the application is in wheat bran liquid state fermentation inoculation of medium Dendritic cladosporium carries out liquid fermentation to obtain fermented feed liquid；Then by fermented feed liquid according to accounting for raw material glutinous rice quality 10~40% Amount is added to vinegar brewing Luo Gang workshop sections, while repeatedly flows plus account for the hair of Raw Materials Rice quality 3~12% in acetic fermentation workshop section Ferment feed liquid.
- 8. application according to claim 7, which is characterized in that contain mass fraction in the wheat bran liquid state fermentation culture medium For 0.5~6% wheat bran, remaining is water, pH 4.8~6.5.
- 9. application according to claim 7, which is characterized in that the application is 72h, 96h, 120h in acetic fermentation Each stream plus one time fermentation feed liquid.
- 10. application according to claim 6, which is characterized in that the vinegar brewing includes rice steeping, steamed rice, drenches meal, falls Cylinder, saccharification, alcoholic fermentation, acetic fermentation, leaching vinegar, sterilization workshop section.
- 11. application according to claim 7, which is characterized in that the vinegar brewing includes rice steeping, steamed rice, drenches meal, falls Cylinder, saccharification, alcoholic fermentation, acetic fermentation, leaching vinegar, sterilization workshop section.
- 12. application according to claim 8, which is characterized in that the vinegar brewing includes rice steeping, steamed rice, drenches meal, falls Cylinder, saccharification, alcoholic fermentation, acetic fermentation, leaching vinegar, sterilization workshop section.
- 13. application according to claim 9, which is characterized in that the vinegar brewing includes rice steeping, steamed rice, drenches meal, falls Cylinder, saccharification, alcoholic fermentation, acetic fermentation, leaching vinegar, sterilization workshop section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510705775.3A CN105238703B (en) | 2015-10-27 | 2015-10-27 | The dendritic cladosporium of one plant height production feruloyl esterase and the application in vinegar brewing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510705775.3A CN105238703B (en) | 2015-10-27 | 2015-10-27 | The dendritic cladosporium of one plant height production feruloyl esterase and the application in vinegar brewing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105238703A CN105238703A (en) | 2016-01-13 |
| CN105238703B true CN105238703B (en) | 2018-05-25 |
Family
ID=55036523
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510705775.3A Active CN105238703B (en) | 2015-10-27 | 2015-10-27 | The dendritic cladosporium of one plant height production feruloyl esterase and the application in vinegar brewing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105238703B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022501A (en) * | 2018-07-13 | 2018-12-18 | 江南大学 | A method of ferulic acid is obtained using waste |
| CN109337821B (en) * | 2018-09-29 | 2022-06-24 | 中国海洋大学 | Cladosporium for producing sterol esterase and enzyme production method |
| CN110066757B (en) * | 2019-04-18 | 2020-08-04 | 江南大学 | Pseudomonas capable of producing feruloyl esterase and application thereof |
| CN111205989B (en) * | 2020-03-11 | 2022-05-17 | 泸州老窖股份有限公司 | Cladosporium sp-1 and application thereof in base wine aging |
| CN111269842B (en) * | 2020-03-11 | 2022-05-17 | 泸州老窖股份有限公司 | Cladosporium sp-2 and application thereof in accelerating aging of new wine |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101054555A (en) * | 2007-03-30 | 2007-10-17 | 南京大学 | An epiphyte capable of digesting agar |
| WO2009078710A1 (en) * | 2007-12-14 | 2009-06-25 | Plant Research International B.V. | Novel micro-organisms controlling plant pathogens |
| CN102796673A (en) * | 2012-08-24 | 2012-11-28 | 黄河三角洲京博化工研究院有限公司 | Feruloyl esterase production strain and method for producing feruloyl esterase by using same |
-
2015
- 2015-10-27 CN CN201510705775.3A patent/CN105238703B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101054555A (en) * | 2007-03-30 | 2007-10-17 | 南京大学 | An epiphyte capable of digesting agar |
| WO2009078710A1 (en) * | 2007-12-14 | 2009-06-25 | Plant Research International B.V. | Novel micro-organisms controlling plant pathogens |
| CN102796673A (en) * | 2012-08-24 | 2012-11-28 | 黄河三角洲京博化工研究院有限公司 | Feruloyl esterase production strain and method for producing feruloyl esterase by using same |
Non-Patent Citations (3)
| Title |
|---|
| Effect of polygalacturonase and feruloyl esterase from Aspergillus tubingensis on demucilage and quality of coffee beans;TAI ES 等;《Process Biochemistry》;20141231;第49卷(第8期);1274-1280 * |
| Production of feruloyl/p-coumaroyl esterase activity by Penicillium expansum, Penicilliumbrevicompactum and Aspergillus niger;DONAGHY JA 等;《Appl Bacteriol》;19951231;第79卷;657-662 * |
| 利用泡盛曲霉产阿魏酸酯酶的研究;方园 等;《广东农业科学》;20111231;第9卷;103-106 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105238703A (en) | 2016-01-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105238703B (en) | The dendritic cladosporium of one plant height production feruloyl esterase and the application in vinegar brewing | |
| CN103416223B (en) | Method for improving cordycepin output in cordyceps militaris fermentation broth | |
| CN107699499B (en) | One Aspergillus oryzae ZA127 and its application | |
| CN106978465B (en) | Fermentation method for improving fermentation yield of total triterpenoids in inonotus obliquus | |
| CN104893983B (en) | Liquid state fermentation low citrinin, the preparation method of High color values monascorubin and product | |
| CN106978350A (en) | One plant of aspergillus niger and its application in the preparation of Pu'er tea chlorins compound | |
| CN109825444B (en) | Strain for improving food safety of red yeast rice yellow wine and brewing method thereof | |
| CN106434369B (en) | One plant of aspergillus oryzae for producing L MALIC ACID and its application | |
| CN103667070A (en) | Plant endophytic fungi of huperzia serrata and application thereof in preparing huperzine A | |
| CN103468463A (en) | Production method of monascus liquid state fermentation yeast | |
| CN108034591A (en) | A kind of purple Monascus and its application | |
| CN107142222A (en) | A kind of culture medium for promoting Morciiella Esculeuta Mycelia to grow | |
| CN107432135A (en) | Promote the method for cynomorium songaricum seed sprouting using fungi | |
| CN103087893A (en) | Preparation method of composite coarse cereals monascus | |
| CN105779299B (en) | Paecilomyces hepiali strain for producing adenosine and mannitol substances and application thereof | |
| CN103815279A (en) | Red yeast rice rich in coenzyme Q10 and preparation method thereof | |
| CN109468232A (en) | One plant of production feruloyl esterase thanks watt bulk bacteria and its application in white wine yeast | |
| CN107488591A (en) | A kind of high enzyme activity, high salt tolerant soy sauce bacterial strain selection and its application process | |
| CN103416222A (en) | Lepista nuda spore cultivation and ripening method through hypha shake flasks and liquid submerged fermentation process | |
| CN103283482B (en) | Cordyceps militaris, cultivation method and separation method | |
| CN106479900B (en) | High yield monascus purpureus penicillium oxalicum Po-25 bacterial strain and application thereof | |
| CN106399121B (en) | A kind of purple red yeast rice bacteria strain | |
| CN109022501A (en) | A method of ferulic acid is obtained using waste | |
| CN103865804B (en) | Beta-glucosidase Producing Strain and the application in resveratrol is prepared in conversion thereof | |
| CN103865803B (en) | Beta-glucosidase Producing Strain and the application prepared in genipin and trans-resveratrol in conversion thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20160113 Assignee: JIANGSU HENGSHUN VINEGAR-INDUSTRY Co.,Ltd. Assignor: Jiangnan University Contract record no.: X2022980001933 Denomination of invention: A Cladosporium Cladosporium strain with high ferulate esterase production and its application in vinegar brewing Granted publication date: 20180525 License type: Exclusive License Record date: 20220228 |