CN105238703A - Cladosporiumc cladosporioides for high yield of feruloyl esterase and application of Cladosporiumc cladosporioides in edible vinegar brewing - Google Patents

Cladosporiumc cladosporioides for high yield of feruloyl esterase and application of Cladosporiumc cladosporioides in edible vinegar brewing Download PDF

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CN105238703A
CN105238703A CN201510705775.3A CN201510705775A CN105238703A CN 105238703 A CN105238703 A CN 105238703A CN 201510705775 A CN201510705775 A CN 201510705775A CN 105238703 A CN105238703 A CN 105238703A
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application
vinegar
feruloyl esterase
wheat bran
fermentation
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CN105238703B (en
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毛健
李翠翠
刘双平
姬中伟
孟祥勇
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Jiangnan University
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Abstract

The invention discloses Cladosporiumc cladosporioides for high yield of feruloyl esterase and an application of Cladosporiumc cladosporioides in edible vinegar brewing, and belongs to the field of fermentation engineering and biotechnology. The Cladosporiumc cladosporioides was preserved by the China Center for Type Culture Collection, the preservation number is CCTCC NO: M2015549, and the preservation date is September, 16th, 2015. According to the invention, wheat bran is used as a fermentation culture medium for fermentation production of high-activity feruloyl esterase, and it is easy to realize industrial production of feruloyl esterase. By applying feruloyl esterase to production of edible vinegar, functional health-care edible vinegar rich in ferulic acid is obtained. Thus, new technical support is provided for development of health-care vinegar.

Description

One plant height produces the dendritic cladosporium of feruloyl esterase and the application in vinegar brewing
Technical field
The present invention relates to a plant height and produce the dendritic cladosporium of feruloyl esterase and the application in vinegar brewing, belong to fermentation engineering and biological technical field.
Background technology
Feruloyl esterase (FEA, EC3.1.1.73) be a subclass of carboxylic ester hydrolase, primary biological function is the ester bond that hydrolyzing plant cell wall polysaccharides and forulic acid (FA) link, thus forulic acid and other cinnamic acid is discharged.At present, the bacterial strain of the product feruloyl esterase of report about has 30 strains, wherein mainly comprise aspergillus niger, aspergillus oryzae, terreus, flavus, aspergillus flavipes, Aspergillus awamori, Tabin aspergillus, Aspergillus nidulans, Aspergillus usamii, head mold, mould, sporotrichum thermophile, Fusarium oxysporum, fusarium prolifertum, Neuraspora crassa, rhizomucor miehei, the basket bacterium of handle, turn green russule, Pleurotus eryngii, Aureobasidium pullulans, anaerobic fungi, new beautiful silk bacterium etc., but the vigor that these bacterial strains produce feruloyl esterase is general lower, is not enough to meet industrial application.Have the correlative study being improved production by biological feruloyl esterase ability by genetic modification means, but the engineering strain output built at present not very high, does not still reach industrialization production requirements yet.Therefore explore enzyme high, safe wild-type feruloyl esterase producing strains alive and seem necessary, have and research and develop value greatly.
Forulic acid is because have the focus that multiple physiological active functions became people's research in recent years always.Report at present, forulic acid has the strong broken effect of going out to hydrogen peroxide, superoxide radical, hydroxy radical qiao, peroxynitrite.Forulic acid, by the effect to endothelin (Endothelin, ET), plays the effect reduced blood pressure.Forulic acid to common cold virus, respiratory syncytial virus and hiv virus have significantly suppress do.Forulic acid can reduce the oxygen-consumption of myocardial ischemia, is used for the treatment of coronary heart diseases and angina pectoris clinically.In recent years, what relevant ferulic acid and its derivatives suppressed colon and rectum carcinoma and tongue cancer is reported in continuous increase.And find forulic acid protect liver, treatment diabetes in have unusual effect.
At present, abroad about being reported in of functional foodstuff of forulic acid is increased gradually, the present invention is intended to utilize Microbial resources biological waste resource to be converted into by fermentation engineering and material can be utilized to add in Vinegar Fermentation system, obtained a kind of functional health vinegar being rich in forulic acid, strengthens the exploitation of health vinegar.
Summary of the invention
Technical problem to be solved by this invention is: lower for current microorganism ferulaic acid esterase activity, the problem of industrial applications cannot be realized, the dendritic cladosporium of the high yield feruloyl esterase providing a strain new, it can utilize Testa Tritici to ferment and produce feruloyl esterase, add in Vinegar Fermentation process first, obtained by be rich in forulic acid vinegar there is anti-oxidant, hypotensive, antithrombotic, reducing blood-fat, antisepsis and anti-inflammation, preventing cancer, protect the effect such as liver, treatment diabetes.
First object of the present invention is to provide the dendritic cladosporium of the new high yield feruloyl esterase of a strain, derives from Zhejiang Province Tapai Shaoxing Wine Co., Ltd's yellow rice wine wheat starter, and through screening, separation and purification obtains.This dendritic cladosporium (Cladosporiumcladosporioides), by China typical culture collection center preservation, be called for short CCTCC, deposit number is CCTCCNO:M2015549, preservation date on September 16th, 2015, preservation address is Wuhan, China Wuhan University.
The flat board observation feature of the dendritic cladosporium of gained of the present invention is as follows: on PDA solid medium, cultivate 72h, bacterial strain bacterium colony is thick, in heavy fleece hair shape, is combined closely, not easily provokes with substratum.Color is just infuscate extremely green and brown look, and the later stage is that chocolate is to black.Bacterium colony back side black.
RDNAITS1-5.8S-ITS2 sequence and the CladosporiumcladosporioidesstrainFR5-SAR1 identity of described dendritic cladosporium reach 100%.
Second object of the present invention is to provide the mould application in production feruloyl esterase of described branch spore.
Described production feruloyl esterase is the main component using wheat bran as substratum.
Described application be by dendritic cladosporium seed liquor according to 3 ~ 8% inoculum size be inoculated in wheat bran liquid state fermentation substratum and cultivate, wheat bran dosage 0.5 ~ 6% (all the other are water), culture temperature 32 ~ 35 DEG C, incubation time 48 ~ 120h, oxygen-supply quantity 0.5 ~ 2vvm, rotating speed 100 ~ 150r/min, pH4.8 ~ 6.5.
NaCl0.3g/L, (NH can also be contained in described wheat bran liquid state fermentation substratum 4) 2sO 41.3g/L, MgSO 47H 2o0.3g/L, K 2hPO 40.3g/L.
3rd object of the present invention is to provide the application of described dendritic cladosporium in vinegar brewing.
Described application is at the dendritic cladosporium of wheat bran liquid state fermentation inoculation of medium, carries out liquid fermenting and obtains fermented feed liquid; Then fermented feed liquid is joined vinegar brewing Luo Gang workshop section according to the amount accounting for raw material glutinous rice quality 10 ~ 40%, simultaneously acetic fermentation workshop section repeatedly stream add the fermented feed liquid accounting for Raw Materials Rice quality 3 ~ 12%.
Described application adds one time fermentation feed liquid at each stream of 72h, 96h, 120h of acetic fermentation.
Described vinegar brewing comprises rice dipping, steamed rice, pouring meal, the cylinder that falls, saccharification, zymamsis, acetic fermentation, pouring vinegar, sterilization workshop section.
Compared with prior art, the invention has the beneficial effects as follows:
1. the invention provides a kind of dendritic cladosporium (Cladosporiumcladosporioides) newly, described bacterial strain has the ability of high yield feruloyl esterase, supplements the database extracting bound form forulic acid bacterium greatly; Described dendritic cladosporium from organic sphere separation and purification out, and be easy to cultivate and preserve, rapidly, energy for growth is strong in breeding;
2. the enzyme work that feruloyl esterase is produced in the dendritic cladosporium fermentation of the present invention can reach 308U/L, can as the novel wild bacterium of extracting bound form phenolic acid from plant;
3., during dendritic cladosporium wheat bran fermented feed liquid first Application is creatively produced to vinegar by the present invention, fully utilized the by product-wheat bran in wheat processing process, achieved the double benefit of exploitation high-quality vinegar and energy-saving and emission-reduction; The use of the mould wheat bran fermented feed liquid of branch spore, under the prerequisite ensureing original vinegar flavor mouthfeel, has the effect significantly promoting ferulaic acid content.
4., by the vinegar that production method of the present invention obtains, in final mensuration vinegar, ferulaic acid content reaches 45 ~ 80ppm, bright color, and alcoholic strength is low, and clean taste is unique, and has certain nourishing function.
Biological deposits information
Dendritic cladosporium, the dendritic cladosporium S2CladosporiumcladosporioidesS2 of taxonomy called after, be preserved in China typical culture collection center on September 16th, 2015, preservation address is Wuhan, China Wuhan University, and deposit number is CCTCCNO:M2015549.
Accompanying drawing explanation
Fig. 1: the colonial morphology of dendritic cladosporium on primary dcreening operation substratum;
Colonial morphology after Fig. 2: dendritic cladosporium PDA separation and purification;
Fig. 3: the HPLC color atlas measuring dendritic cladosporium fermented liquid.
Embodiment
Culture medium prescription:
Primary dcreening operation substratum: NaCl0.3g, (NH 4) 2sO 41.3g, MgSO 47H 2o0.3g, K 2hPO 40.3g, agar powder 18g, 15ml Ferulic acid ethylester (being dissolved in 10%V/V dimethyl formamide), distilled water 1000ml, pH nature, 121 DEG C of sterilizing 20min.
Pure medium: potato agar solid medium (PDA solid medium), potato 200g, sucrose 20g, agar 18g, distilled water 1000ml, pH nature, 121 DEG C of sterilizing 20min.
Seed culture medium: liquid PDA substratum, does not add agar, pH nature, 121 DEG C of sterilizing 20min.
Sieve substratum again: NaCl0.3g, (NH 4) 2sO 41.3g, MgSO 47H 2o0.3g, K 2hPO 40.3g, distilled water 1L, pH6.5.In 250mL triangular flask, take 10g wheat bran, then add 100mL liquid portion, in 121 DEG C of sterilizing 20min, obtain multiple sieve substratum.The inoculum size being 4% by volume by the seed liquor of cultivating 24h is inoculated into 100mL and sieves again in substratum, in 30 DEG C, cultivates certain hour under 200r/min.
Embodiment 1: the screening of cladosporium sp strain
The primary dcreening operation of dendritic cladosporium, by taking Ferulic acid ethylester as sole carbon source, producing transparent circle phenomenon and filtering out the bacterial strain possessing and produce feruloyl esterase ability.
Take vinegar wheat koji sample from Zhejiang Province Tapai Shaoxing Wine Co., Ltd, get wheat koji 10g, be placed in the 250mL triangular flask containing 90ml sterile distilled water and granulated glass sphere, sealing, 200r/min jolting 30min on shaker, makes wherein microorganism fully disperse, makes 10 -1times sample diluting liquid.
Bechtop obtains 10 by gradient dilution method -2, 10 -3, 10 -4, 10 -5, 10 -6, 10 -7times sample diluting liquid, gets 10 -4, 10 -5, 10 -6, 10 -7four each 0.2mL of dilution gradient sample liquid coat primary dcreening operation substratum, are placed on after cultivating 3d in 28 DEG C of incubators, observe transparent circle size.
After primary dcreening operation is cultivated, according to the size (Fig. 1 is the colonial morphology of dendritic cladosporium of the present invention on primary dcreening operation substratum) of periphery of bacterial colonies transparent circle on primary dcreening operation substratum, the bacterium colony that picking transparent circle is large carries out gradient dilution, coat on primary dcreening operation flat board, cultivate 2d in 28 DEG C of incubators, obtain producing the single bacterium colony of microorganism of feruloyl esterase.Get single colony inoculation in PDA substratum, cultivate 3d in 28 DEG C of incubators, glycerine-80 DEG C of conservations, 4 DEG C, inclined-plane conservation.
By the primary dcreening operation inoculation of separation and purification in multiple sieve substratum, carrying out multiple sieve by measuring ferulaic acid content height in multiple sieve substratum, obtaining the bacterial strain of high yield feruloyl esterase, measuring its enzyme and live as 308U/L.
Embodiment 2: the Microbiological Characteristics of bacterial strain and qualification
As shown in Figure 2, bacterial strain bacterium colony is thick for the result of bacterial strain on PDA flat board after separation and purification, in heavy fleece hair shape, is combined closely, not easily provokes with substratum.Color is just infuscate extremely green and brown look, and the later stage is that chocolate is to black.Bacterium colony back side black.Tentatively be judged as mould.
Extracting bacterial strain STb gene as template, pcr amplification rDNAITS1-5.8S-ITS2 district, is 520bp, GenBank sequence number: KP900248.1 through order-checking goal gene sequence length.The rDNAITS1-5.8S-ITS2 sequence of bacterial strain and the identity (Identity) of 10 strain bacterial strains are all more than 90.0%, and reach 100% with CladosporiumcladosporioidesstrainFR5-SAR1 identity wherein, the molecular evolutionary trees built is shown in accompanying drawing, bacterial strain S2 and Cladosporiumcladosporioides is on the same branch of the evolutionary tree built, in conjunction with identification of morphology and the rDNAITS1-5.8S-ITS2 the sequencing results of bacterial strain, judge that bacterial strain of the present invention is as dendritic cladosporium sp strain.
Embodiment 3: the application of bacterial strain wheat bran fermented liquid in vinegar is produced
Wheat bran 0.5kg, distilled water 10L, pH4.8, logical oxygen 1vvm, rotating speed 150r/min, under temperature 28 DEG C of conditions, ferments, get the liquid fermentation liquid of fermentation 76h, utilize high-efficient liquid phase color spectrometry ferulaic acid esterase activity (as shown in Figure 3), enzyme is lived as 280U/L.
Chromatographic column: XBridgeTMC18 (4.6 × 250mm, 5 μm)
Moving phase: (the B:0.01% glacial acetic acid aqueous solution; Moving phase D: pure acetonitrile).Flow velocity 1.0mL/min.
Gradient elution method: initial time, B solution flow 80%, solution D flow 20%, maintains 2min; 8min, B solution flow 72%, solution D flow 28%; 13min, B solution flow 42%, solution D flow 58%; 19min, B solution flow 80%, solution D flow 20%, maintains 6min, terminates.The linearity curve obtained is: Y=2.79 × 10 4x+6.65 × 10 3, R 2=0.9982.
Ferulaic acid esterase activity measures
Determination step: get 250 μ L fermentation crude enzyme liquids, add 250 μ L, pH6.0Na 2hPO 4-C 6h 8o 7the Ferulic acid methylester solution of buffer preparation, after 50 DEG C of reaction 10min, add 500 μ L10% glacial acetic acids (V/V), 4 DEG C, the centrifugal 20min of 10000r/min, cross 0.22 μm of filter membrane, HPLC method measures ferulaic acid content.
Blank is that distilled water 250 μ L replaces Ferulic acid methylester solution, and other reaction is identical with mensuration group.
Enzyme is lived definition: 50 DEG C, under pH6.0 condition, per minute esterlysis Ferulic acid methylester, generates enzyme amount needed for 1 μm of ol forulic acid and is defined as 1 enzyme activity unit (U).
By fermented feed liquid according to 30% amount (accounting for the per-cent of raw material glutinous rice quality) join vinegar brewing Luo Gang workshop section, simultaneously at acetic fermentation workshop section 72h, 96h, 120h, continue the fermented feed liquid that stream adds 5% (accounting for the per-cent of Raw Materials Rice quality).
Finally record ferulaic acid content in vinegar and reach 57ppm.
Embodiment 4: the contrast of this product and zhenjiang vinegar physical and chemical index
The functional vinegar obtain embodiment 3 and the physical and chemical index of zhenjiang vinegar, result is as shown in table 1.Analyzed known by table 1, embodiment 3 produce the zhenjiang vinegar that vinegar belongs to total acid >=5.00g/L, ferulaic acid content improves more than 9 times.
Table 1 vinegar physical and chemical index contrasts
Embodiment 5: fermented feed liquid addition and the time of interpolation are on the impact of finished product vinegar physical and chemical index
Join in vinegar brewing process by fermented feed liquid according to Different adding amount and interpolation time, the finished product vinegar physical and chemical index of acquisition is to such as table 2.Analyzed by table 2 known, add the vinegar ferulaic acid content of fermented feed liquid production all at more than 45ppm according to the method for the invention, and other physical and chemical index of vinegar is all good, reaches zhenjiang vinegar standard.
Table 2 vinegar physical and chemical index contrasts
Although the present invention with preferred embodiment openly as above; but it is also not used to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; all can do various changes and modification, what therefore protection scope of the present invention should define with claims is as the criterion.

Claims (10)

1. a dendritic cladosporium, is characterized in that, described dendritic cladosporium is preserved in China typical culture collection center on September 16th, 2015, and preservation address is Luojiashan, Wuchang, Wuhan City, Hubei Province Wuhan University, and deposit number is CCTCCNO:M2015549.
2. method according to claim 1, is characterized in that, described dendritic cladosporium screening is from yellow rice wine wheat starter.
3. dendritic cladosporium described in claim 1 is producing the application in feruloyl esterase.
4. application according to claim 3, is characterized in that, described production feruloyl esterase is the main component using wheat bran as substratum.
5. application according to claim 3, it is characterized in that, described application be by dendritic cladosporium seed liquor according to 3 ~ 8% inoculum size be inoculated in wheat bran liquid state fermentation substratum and cultivate, wheat bran dosage 0.5 ~ 6%, culture temperature 32 ~ 35 DEG C, incubation time 48 ~ 120h, oxygen-supply quantity 0.5 ~ 2vvm, rotating speed 100 ~ 150r/min, pH4.8 ~ 6.5.
6. the application of dendritic cladosporium in vinegar brewing described in claim 1.
7. application according to claim 6, is characterized in that, described application is at the dendritic cladosporium of wheat bran liquid state fermentation inoculation of medium, carries out liquid fermenting and obtains fermented feed liquid; Then fermented feed liquid is joined vinegar brewing Luo Gang workshop section according to the amount accounting for raw material glutinous rice quality 10 ~ 40%, simultaneously acetic fermentation workshop section repeatedly stream add the fermented feed liquid accounting for Raw Materials Rice quality 3 ~ 12%.
8. application according to claim 7, is characterized in that, containing massfraction in described wheat bran liquid state fermentation substratum is the wheat bran of 0.5 ~ 6%, and all the other are water, pH4.8 ~ 6.5.
9. application according to claim 7, is characterized in that, described application adds one time fermentation feed liquid at each stream of 72h, 96h, 120h of acetic fermentation.
10., according to the arbitrary described application of claim 6-9, it is characterized in that, described vinegar brewing comprises rice dipping, steamed rice, pouring meal, the cylinder that falls, saccharification, zymamsis, acetic fermentation, pouring vinegar, sterilization workshop section.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109022501A (en) * 2018-07-13 2018-12-18 江南大学 A method of ferulic acid is obtained using waste
CN109337821A (en) * 2018-09-29 2019-02-15 中国海洋大学 A kind of cladosporium and enzyme producing method producing sterol esterase
CN110066757A (en) * 2019-04-18 2019-07-30 江南大学 One plant of pseudomonad for producing feruloyl esterase and its application
CN111205989A (en) * 2020-03-11 2020-05-29 泸州老窖股份有限公司 Cladosporium sp-1 and application thereof in base wine aging
CN111269842A (en) * 2020-03-11 2020-06-12 泸州老窖股份有限公司 Cladosporium sp-2 and application thereof in accelerating aging of new wine

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101054555A (en) * 2007-03-30 2007-10-17 南京大学 An epiphyte capable of digesting agar
WO2009078710A1 (en) * 2007-12-14 2009-06-25 Plant Research International B.V. Novel micro-organisms controlling plant pathogens
CN102796673A (en) * 2012-08-24 2012-11-28 黄河三角洲京博化工研究院有限公司 Feruloyl esterase production strain and method for producing feruloyl esterase by using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101054555A (en) * 2007-03-30 2007-10-17 南京大学 An epiphyte capable of digesting agar
WO2009078710A1 (en) * 2007-12-14 2009-06-25 Plant Research International B.V. Novel micro-organisms controlling plant pathogens
CN102796673A (en) * 2012-08-24 2012-11-28 黄河三角洲京博化工研究院有限公司 Feruloyl esterase production strain and method for producing feruloyl esterase by using same

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DONAGHY JA 等: "Production of feruloyl/p-coumaroyl esterase activity by Penicillium expansum, Penicilliumbrevicompactum and Aspergillus niger", 《APPL BACTERIOL》 *
TAI ES 等: "Effect of polygalacturonase and feruloyl esterase from Aspergillus tubingensis on demucilage and quality of coffee beans", 《PROCESS BIOCHEMISTRY》 *
方园 等: "利用泡盛曲霉产阿魏酸酯酶的研究", 《广东农业科学》 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109022501A (en) * 2018-07-13 2018-12-18 江南大学 A method of ferulic acid is obtained using waste
CN109337821A (en) * 2018-09-29 2019-02-15 中国海洋大学 A kind of cladosporium and enzyme producing method producing sterol esterase
CN109337821B (en) * 2018-09-29 2022-06-24 中国海洋大学 Cladosporium for producing sterol esterase and enzyme production method
CN110066757A (en) * 2019-04-18 2019-07-30 江南大学 One plant of pseudomonad for producing feruloyl esterase and its application
CN110066757B (en) * 2019-04-18 2020-08-04 江南大学 Pseudomonas capable of producing feruloyl esterase and application thereof
CN111205989A (en) * 2020-03-11 2020-05-29 泸州老窖股份有限公司 Cladosporium sp-1 and application thereof in base wine aging
CN111269842A (en) * 2020-03-11 2020-06-12 泸州老窖股份有限公司 Cladosporium sp-2 and application thereof in accelerating aging of new wine
CN111205989B (en) * 2020-03-11 2022-05-17 泸州老窖股份有限公司 Cladosporium sp-1 and application thereof in base wine aging
CN111269842B (en) * 2020-03-11 2022-05-17 泸州老窖股份有限公司 Cladosporium sp-2 and application thereof in accelerating aging of new wine

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