CN1344727A - Multifunctional mycoprotein product for plant and its prepn - Google Patents
Multifunctional mycoprotein product for plant and its prepn Download PDFInfo
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- CN1344727A CN1344727A CN 01128666 CN01128666A CN1344727A CN 1344727 A CN1344727 A CN 1344727A CN 01128666 CN01128666 CN 01128666 CN 01128666 A CN01128666 A CN 01128666A CN 1344727 A CN1344727 A CN 1344727A
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- 108090000623 proteins and genes Proteins 0.000 claims abstract description 29
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 26
- 244000000004 fungal plant pathogen Species 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims description 16
- 230000002538 fungal effect Effects 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 3
- 239000007979 citrate buffer Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000008057 potassium phosphate buffer Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 230000000295 complement effect Effects 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims 1
- 238000013467 fragmentation Methods 0.000 claims 1
- 238000006062 fragmentation reaction Methods 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 6
- 230000012010 growth Effects 0.000 abstract description 5
- 241000233866 Fungi Species 0.000 abstract description 3
- 230000001717 pathogenic effect Effects 0.000 abstract description 3
- 241001465180 Botrytis Species 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 25
- 238000011081 inoculation Methods 0.000 description 18
- 201000010099 disease Diseases 0.000 description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 13
- 235000013399 edible fruits Nutrition 0.000 description 13
- 238000013016 damping Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 238000005507 spraying Methods 0.000 description 7
- 241000227653 Lycopersicon Species 0.000 description 5
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 5
- 206010039509 Scab Diseases 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 239000000575 pesticide Substances 0.000 description 4
- 230000009261 transgenic effect Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000003449 preventive effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 244000241257 Cucumis melo Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 241000628997 Flos Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 241000223600 Alternaria Species 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 241000588694 Erwinia amylovora Species 0.000 description 1
- 244000186654 Fockea angustifolia Species 0.000 description 1
- 235000015210 Fockea angustifolia Nutrition 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241001330975 Magnaporthe oryzae Species 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241000228150 Penicillium chrysogenum Species 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 241000233622 Phytophthora infestans Species 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- 241000187391 Streptomyces hygroscopicus Species 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002052 anaphylactic effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- -1 glucoside compounds Chemical class 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000020978 protein processing Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention is one kind of protein product extracted from microbe, especially plant pathogenic funqi. The said protein product includes active protein with molecular weight of 42-60 KD and assistant active protein with molecular weight of 30 and 20 KD. The fungus protein is prepared with botrytis and other plant pathogenic fungi and through culture, concentration, crushing, killing live fungi, denaturing unsatable and non-active protein and eccentric purification. Great amount field test shows that the product can strengthen the resistance of various crops and promote crop growth without producing ecological and environment problem.
Description
Technical field: the present invention relates to the protein product that a kind of protein product that is extracted by microorganism, especially pathogenic fungi extract.
Background technology: being extensive use of of chemical fertilizer and agricultural chemicals makes a great contribution to agricultural development, is still one of main means that improve crop yield so far.But life-time service chemical pesticide and chemical fertilizer have also brought such as problems such as environmental pollution, ecological damage, sick worm resistance and food safeties, are seriously restricting the Sustainable development of agricultural.Bio-feritlizer, biological pesticide and transgenic plant are major measure of contemporary crop high-quality, efficient, nuisanceless production.Biological pesticide in the past all is that bio-extract or secretory product itself directly play a role, as jinggangmeisu is exactly water soluble antibiotics---the direct killing pathogenic bacteria of glucoside compounds that is produced by the mutation of streptomyces hygroscopicus well hilllock, still welcome on producing so far.Modern age molecular biotechnology fast development, producing biological pesticide and transgenic plant by modes such as gene clone, conversions becomes the focus that people pay close attention to.In recent years the research of BT and PI transgenic plant is succeedd.But the safety issue of transgenic plant is subjected to scientist's great attention, and many countries strictly control the application of this class material.China scientific worker finds that the various plants extract induces potato that the resistance of phytophthora infestans is had good effect, and the disease protection ratio reaches 54%, and can promote that potato seed is sprouted, early growth.The ZhongminWei of Cornell Univ USA finds a kind of protein (Harpin) that causes allergic reaction of plant that plant pathogenetic bacteria Erwinia amylovora produces, molecular weight is 40kd, form by 385 amino acid, the expression of series of genes in the energy inducing plant body, the growing system of activated plant self and defense mechanism, thereby resist the invasion and attack of common disease insect pest and the influence of poor environment, have the root of hair of increasing amount, leaf area, setting percentage simultaneously, effects such as premature ripening and volume increase.U.S. EDEN Biological Science Co., Ltd uses this technological development to go out broad spectrum harmless boilogical agricultural chemicals Messenger, on multiple field crop, cash crop, use, effect is all very obvious, generally can reduce medication 50-80%, volume increase 10-20%, find that now plant pathogenetic bacteria all has the hrp gene cluster, molecular weight 20-40kd comprises 3-13 gene.A lot of cause of diseases of phytophthora (Phophthora) can be secreted and be induced non-host to produce anaphylactoid protein, are called Elictin, and molecular weight is 10.6KD, and it can also bring out various plants simultaneously and produce multiple defense response, improves the disease resistance of these plants.But can other plant pathogenic fungi produce similar protein on host and non-host, do not appear in the newspapers as yet.
Summary of the invention: the present invention is intended to from plant pathogenic fungi the separation and Extraction plant and uses multifunctional protein, the self-defense system and the growing system of inducing plant, thus the invasion and attack of disease and pest resisted, promote growth and development of plants simultaneously, improve crop yield, and promote fruit fresh-keeping.
The present invention is such realization: plant is used multi-functional true mycoprotein goods, be a kind of from plant pathogenic fungi extraction separation, the protein of inducing plant information conduction, it has comprised molecular weight between 42-60KD, active 4 kinds of activated proteins of complementary and molecular weight are the auxiliary activity albumen of 30KD and 20KD, plant pathogenic fungi is cultivated through secondary in conventional nutrient solution, during to the fungal spore maximum, with 5000rpm centrifugal 30 minutes, the spore of collecting is pressed 5mM potassium phosphate buffer or the citrate buffer solution that nutrient solution volume 1/10 adds PH6.8, fungal spore suspension adopts ultrasonic wave 30 minutes or the broken spore cell of 180bar high pressure, centrifugal 30 minutes once more with 5000rpm, remove sedimentary smudge cells tissue, supernatant liquor is promptly made the mycoprotein goods with 100 degree boiling water water-baths 15 minutes.
Be described in further detail the present invention below:
With plant pathogenic fungi Staphlosporonites (Botrytis), interlink spore genus (Alternaria), rice blast fungus (Pyrcularia), poison mould (Penicillium), Aspergillus flavus (Asporgillus), in sheath blight fungus (Rhizoctonia solani) and the Penicillium notatum (Fusarium) any is material, in conventional nutrient solution, cultivate through secondary, during to the fungal spore maximum, through 5000rpm centrifugal 30 minutes, the spore of collecting is pressed 5mM potassium phosphate buffer or the citrate buffer solution that nutrient solution volume 1/10 adds PH6.8, fungal spore suspension adopts ultrasonic wave 30 minutes or the broken spore cell of 180bar high pressure, centrifugal 30 minutes once more with 5000rpm, remove sedimentary smudge cells tissue, keep the mycoprotein in the supernatant liquor, supernatant liquor is spent boiling water water-baths 15 minutes with 100, to kill viable bacteria, make unstable and inactive protein sex change, through the supernatant liquor of water-bath centrifugal 30 minutes again with 5000rpm, the viable bacteria that removal is killed and the albumen of sex change, the supernatant liquor that keeps is mycoprotein, these mycoprotein goods are that molecular weight is 4 kinds of activated proteins between the 42-60KD, and molecular weight is the auxiliary activity albumen of 30KD and 20KD.
Mycoprotein is by the plant surface protein receptor, but the conduction of the information of inducing plant starts a series of metabolic reactions in the plant materials, cause genetic expression, activated plant self-defence system and growing system, thus generation is to the resistance of disease and pest, promote plant-growth, improve crop yield.Mycoprotein of the present invention can be made into formulations such as liquid and pulvis, be used for plant seed dressing, soak seed, water root and foliage-spray, working concentration is 10-500ug/ml, indoor pot and field test through 40 various plants, show that the mycoprotein effect is comprehensive, fungal disease, Micobial Disease, virus disease and insect pest (seeing Table 1-table 5) can not only be prevented and treated, and plant growth (seeing Table 6-table 9) can be significantly promoted; According to incompletely statistics, mycoprotein reaches 40-80% to the anti-efficient of disease and pest, and the crop yield rate reaches 4-85%.Production technique of the present invention is simple, and cost is lower, and the lasting period is long, can be widely used in efficient, the high-quality of crop, nuisanceless production and fruit fresh-keeping.
Table 1 is handled the effect that Flos Pelargonii is induced anti-grey mold fungal disease with Fungal Protein
| Handle | Inoculation | Effect % | ||
| The same day grey mold-10ug/ml grey mold-20ug/ml mould-10ug/ml mould-20ug/rml damping fluid (5mMKPO 4) | Inoculation inoculation inoculation inoculation inoculation in the 7th day | The 13rd day 73.08 76.92 38.46 42.31 0 | The 15th day 62.86 71.43 37.14 40.00 0 | The 20th day 60.98 63.41 27.95 34.15 0 |
Annotate: the T-test shows: the level 95% and 99%, there are statistical significant difference in Fungal Protein and damping fluid between handling.
Table 2 Fungal Protein is handled the effect that tomato is induced resistance to bacterial wilt
Annotate: the T-test shows: on 95% and 99% level, have statistical significant difference between Fungal Protein and the damping fluid.
| Handle | Inoculation | Effect % | ||
| Grey mold 20ug/ml double cross on same day pink mold 20ug/ml mould 20ug/ml damping fluid (5mMKPO 4) | Inoculation inoculation inoculation inoculation in the 7th day | The 13rd day 76.92 61.54 38.46 0 | The 15th day 72.73 54.55 31.82 0 | The 20th day 70.97 54.84 29.03 0 |
Table 3 Fungal Protein is handled the effect that Flos Pelargonii is induced anti-xanthomonas disease
| Handle | Inoculation | Effect % | ||
| The same day grey mold-10ug/ml grey mold-20ug/ml double cross pink mold-10ug/ml double cross pink mold-10ug/ml damping fluid (5mMKPO 4) | Inoculation inoculation inoculation inoculation inoculation in the 7th day | The 13rd day 75.00 83.33 58.33 66.67 0 | The 15th day 75.00 78.57 57.14 64.29 0 | The 20th day 65.12 77.57 51.16 60.47 0 |
Annotate: the T-test shows: on 95% and 99% level, have statistical significant difference between Fungal Protein and the damping fluid.
Back 4 days evoking tobacco mosaic disease resistance effects of inoculation in the 7th day behind the table 4 spray Fungal Protein
| Sample | Spraying leaf 1 TMV scab number | Spraying leaf 2 TMV scab numbers | Spraying leaf 3 TMV scab numbers | The average scab number of spraying leaf | Spraying % effect | Spraying P＜0.05 | Spraying P＜0.01 |
| Grey mold-10ug/ml grey mold-20ug/ml double cross pink mold-10ug/ml double cross pink mold-20 ug/ml mould-10ug/ml mould-20ug/ml buffer solution (5mMKPO4) | ?????7 ?????6 ?????9 ?????8 ?????23 ?????21 ?????64 | ????7 ????7 ????12 ????8 ????27 ????19 ????62 | ????8 ????7 ????10 ????11 ????28 ????23 ????61 | ????7.33 ????6.67 ????10.33 ????9.00 ????26.00 ????21.00 ????62.33 | 88.24 89.30 83.42 85.56 58.29 66.31 do not have | Significantly or not | Significantly or not |
Table 5 mycoprotein is to the influence of tomato virus disease and fruit disease
Annotate: decayed fruit comprises all of scab fruits on the fruit, the disease species that causes is a kind of incessantly.
| Handle | Virus disease morbidity number (240 strain) | Preventive effect (%) | Dead strain number | Preventive effect (%) | All kinds of fruits decayed fruit number | Preventive effect (%) |
| ?60ug/ml ?30ug/ml ?15ug/ml ????CK | ????0.30 ????0.67 ????2.30 ????19.30 | ??98.4 **??96.5 **??88.0 ** | ????0 ????0 ????0 ????3.3 | ????100 ????100 ????100 | ??11.33 ??13.67 ??17.00 ??28.30 | ???60.1 ???51.6 ???39.9 |
Table 6 is handled seedling altitude effect after the tomato with Fungal Protein
| Handle | Efficient % | Efficient % | Efficient % |
| The 1st day grey mold-10ug/ml grey mold-20ug/ml mould-10ug/ml mould-slow (5mMKPO of 20ug/ml buffering4) | Do not have in the 14th day 9.52 11.43 6.67 6.67 | Do not have in the 18th day 10.14 12.16 6.08 7.43 | Do not have in the 21st day 10.28 13.08 6.07 7.48 |
Annotate: originally be treated to mycoprotein and soak one night of tomato seed, and in 21 °-24 ℃ growth indoor raising of seedling.The T-test shows: between Fungal Protein processing and damping fluid processing, on 95% and 99% level statistical significant difference is arranged.
Table 7 mycoprotein is to the influence of yield of hot pepper
| Handle | Mean yield (jin) | Average fruit number (individual) | The average weight/really (jin) | Comparison photograph ± % (mean yield) | Comparison photograph ± % (average fruit number) |
| ?60ug/ml ?30ug/ml ?15ug/ml ????CK | ????49.3 ????45.3 ????42.4 ????31.1 | ????294 ????278 ????259 ????217 | ???0.072 ???0.072 ???0.072 ???0.066 | ????58.5 ????45.7 ????36.3 | ????35.5 ????28.1 ????19.4 |
The influence of table 8 pair The yield of watermelon
| Handle | The real mean yield of receiving in 7/13-16 sub-district | The average melon and fruit number in 7/13-16 sub-district | Average single heavy (jin) | Comparison photograph ± % (the average actual quantities in sub-district) | Comparison photograph ± % (the average single melon in sub-district is heavy) |
| ??60ug/ml ??30ug/ml ??15ug/ml ?????CK | ????157.8 **????156.1 **????141.6 **??????97 | ????19.7 ????19.7 ????20 ????14 | ????8 ????7.9 ????7.4 ????4.8 | ?????66.50 ?????60.76 ?????49.02 | ?????66.67 ?????64.58 ?????54.17 |
The influence of table 9 pair tomato output
| Handle | Mean yield (jin/sub-district) | Mean fruit weight (jin) | Comparison photograph ± % output | Comparison photograph ± % single fruit weight |
| ?60ug/ml ?30ug/ml ?15ug/ml ????CK | ????1001 ????955 ????858 ????546 | ????1.15 ????0.98 ????0.86 ????0.55 | ??????83.3 ??????74.9 ??????57.3 | ??????107.1 ??????77.4 ??????55.5 |
Table 10 mycoprotein is to the influence of young rice seedlings growth
| Handle | Height of seedling (cm) (15 basins are average) | Plant dry weight (g) (6 basins are average) |
| Mycoprotein 440 ug/ml CK | ????15.22 **????13.73 | ????0.32 *????0.29 |
Claims (2)
1, kind of plant multi-functional true mycoprotein goods, the inducing plant information conductive protein matter that it is characterized in that extraction separation from plant pathogenic fungi, it comprised molecular weight between 42-60KD, 4 kinds of activated proteins of active complementary and molecular weight be the auxiliary activity albumen of 30KD and 20KD.
2, a kind of plant as claimed in claim 1 is used the preparation method of multi-functional true mycoprotein goods, comprise the cultivation of fungal spore, concentrate, fragmentation and purifying, it is characterized in that plant pathogenic fungi cultivates through secondary in conventional nutrient solution, during to the fungal spore maximum, with 5000rpm centrifugal 30 minutes, the spore of collecting is by nutrient solution volume 1/10, the 5mM potassium phosphate buffer or the citrate buffer solution that add PH6.8, fungal spore suspension adopts ultrasonic wave 30 minutes or the broken spore cell of 180bar high pressure, centrifugal 30 minutes once more with 5000rpm, remove sedimentary smudge cells tissue, supernatant liquor is promptly made the mycoprotein goods with 100 degree boiling water water-baths 15 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011286660A CN1142179C (en) | 2001-10-09 | 2001-10-09 | Multifunctional mycoprotein product for plant and its prepn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011286660A CN1142179C (en) | 2001-10-09 | 2001-10-09 | Multifunctional mycoprotein product for plant and its prepn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1344727A true CN1344727A (en) | 2002-04-17 |
| CN1142179C CN1142179C (en) | 2004-03-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011286660A Expired - Fee Related CN1142179C (en) | 2001-10-09 | 2001-10-09 | Multifunctional mycoprotein product for plant and its prepn |
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| Country | Link |
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| CN (1) | CN1142179C (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305374C (en) * | 2005-04-15 | 2007-03-21 | 中国农业科学院农业环境与可持续发展研究所 | Protein for promoting plant growth and enhancing disease resistance of plant, and synthetic gene |
| CN1331401C (en) * | 2006-01-13 | 2007-08-15 | 长沙普绿通生物科技有限公司 | Biological seed coating agent |
| CN100346700C (en) * | 2006-01-13 | 2007-11-07 | 长沙普绿通生物科技有限公司 | Biological medicament fertilizer |
| CN101153057B (en) * | 2006-09-27 | 2010-05-19 | 中国农业科学院植物保护研究所 | Protein for improving plants fastness and accelerating plant growth and encoding gene thereof |
| CN102028000A (en) * | 2010-11-01 | 2011-04-27 | 北京丰原康泰生物科技有限公司 | Application of nascent polypeptide-associated complex (NAC) protein as vegetable immune protein |
| CN102648713A (en) * | 2011-02-23 | 2012-08-29 | 天津市植物保护研究所 | Technology for improving biological disease-prevention activity of trichoderma preparation by utilizing fungi activator protein |
| CN102823626A (en) * | 2012-09-20 | 2012-12-19 | 联保作物科技有限公司 | Fungus biological protein water dispersible granules |
| CN102972448A (en) * | 2012-12-25 | 2013-03-20 | 联保作物科技有限公司 | Disease-resistant composition, and application and suspended seed coating agent thereof |
| CN104738094A (en) * | 2013-12-31 | 2015-07-01 | 北京绿色农华植保科技有限责任公司 | Plant immune induced resistance protein composition as well as preparation method and application thereof |
-
2001
- 2001-10-09 CN CNB011286660A patent/CN1142179C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305374C (en) * | 2005-04-15 | 2007-03-21 | 中国农业科学院农业环境与可持续发展研究所 | Protein for promoting plant growth and enhancing disease resistance of plant, and synthetic gene |
| CN1331401C (en) * | 2006-01-13 | 2007-08-15 | 长沙普绿通生物科技有限公司 | Biological seed coating agent |
| CN100346700C (en) * | 2006-01-13 | 2007-11-07 | 长沙普绿通生物科技有限公司 | Biological medicament fertilizer |
| CN101153057B (en) * | 2006-09-27 | 2010-05-19 | 中国农业科学院植物保护研究所 | Protein for improving plants fastness and accelerating plant growth and encoding gene thereof |
| CN102028000A (en) * | 2010-11-01 | 2011-04-27 | 北京丰原康泰生物科技有限公司 | Application of nascent polypeptide-associated complex (NAC) protein as vegetable immune protein |
| CN102028000B (en) * | 2010-11-01 | 2014-07-02 | 普绿通（北京）生物科技有限公司 | Application of nascent polypeptide-associated complex (NAC) protein as vegetable immune protein |
| CN102648713A (en) * | 2011-02-23 | 2012-08-29 | 天津市植物保护研究所 | Technology for improving biological disease-prevention activity of trichoderma preparation by utilizing fungi activator protein |
| CN102823626A (en) * | 2012-09-20 | 2012-12-19 | 联保作物科技有限公司 | Fungus biological protein water dispersible granules |
| CN102972448A (en) * | 2012-12-25 | 2013-03-20 | 联保作物科技有限公司 | Disease-resistant composition, and application and suspended seed coating agent thereof |
| CN102972448B (en) * | 2012-12-25 | 2014-10-22 | 联保作物科技有限公司 | Disease-resistant composition, and application and suspended seed coating agent thereof |
| CN104738094A (en) * | 2013-12-31 | 2015-07-01 | 北京绿色农华植保科技有限责任公司 | Plant immune induced resistance protein composition as well as preparation method and application thereof |
| CN104738094B (en) * | 2013-12-31 | 2017-11-24 | 北京绿色农华作物科技有限公司 | A kind of plant immune lures anti-protein composition and its preparation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1142179C (en) | 2004-03-17 |
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