CN105706741B - The method of Quantitative Determination of Ergosterol and its obtained mushroom in a kind of raising agaricus bisporus - Google Patents
The method of Quantitative Determination of Ergosterol and its obtained mushroom in a kind of raising agaricus bisporus Download PDFInfo
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- CN105706741B CN105706741B CN201610223425.8A CN201610223425A CN105706741B CN 105706741 B CN105706741 B CN 105706741B CN 201610223425 A CN201610223425 A CN 201610223425A CN 105706741 B CN105706741 B CN 105706741B
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- agaricus bisporus
- ergosterol
- quantitative determination
- inducer
- meja
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The present invention relates to a kind of methods of Quantitative Determination of Ergosterol in raising agaricus bisporus, belong to food nutrition technical field.The present invention carries out sprinkling processing using methyl jasmonate (MeJA) ethanol-water solution during cultivation of agaricus bisporus, it is obviously improved its Quantitative Determination of Ergosterol, improve the nutritive value and functionality edible quality of agaricus bisporus, agaricus bisporus cell integrity energy is helped to improve simultaneously, conducive to the fresh-keeping and transport after adopting, it how stable and improve Quantitative Determination of Ergosterol this problem in science in agaricus bisporus is, provide green, it is economical, it is convenient, the theories integration and settling mode easily promoted, the new approaches of edible fungus functional nutrient substance accumulation and Investigation on intensification are opened up.
Description
One, technical field
The present invention relates to a kind of methods of Quantitative Determination of Ergosterol in raising agaricus bisporus, belong to food nutrition technical field,
It is related to the external source inductive technology of function factor in edible fungus.
Two, background technique
Agaricus bisporus (Agaricus bisporus) is used as a kind of edible fungi of large size, is to cultivate most wide, production in the world
Amount at most, a kind of most common edible fungus of consumption, cultivation of agaricus bisporus technology in China's was quickly grown in recent years, yield and was gone out
Mouth amount surmounts the U.S. and leaps to the first in the world.Ergosterol (Ergosterol) is also known as ergosterol, is that one kind is primarily present in
Small molecule secondary metabolite in yeast and fungi, is important VDThe important feature substance of original and fungal plasma membranes,
In numerous edible fungus, the Quantitative Determination of Ergosterol highest (7-10mg/g DW) of agaricus bisporus.Currently, ergosterol is demonstrate,proved
It is real to have the function of to alleviate diabetes, reduction cholesterol and the various actives such as antitumor, and eucaryotic cell structure integrality can be maintained, it helps
Rear preservation is adopted in edible fungus.Although the content of ergosterol is net compared with other edible fungus more horn of plenty in agaricus bisporus
Content is also no more than 10mg/g DW.Therefore, promote Quantitative Determination of Ergosterol for improving the nutritive value of agaricus bisporus, enhancing it
There is important scientific meaning and application and popularization value to the health-care effect of consumer.
About the research of Quantitative Determination of Ergosterol in edible fungus, focuses primarily upon obtain wheat using genetic engineering means before this
Angle sterol high yield microbial strains, and by the biosynthesis of control technological parameter promotion ergosterol, but its technical matters is numerous
It is trivial, it still needs to handle by multiprogramming before obtaining matured product, is unable to direct marketing and eats, to not obtain extensive technology
It promotes.In recent years, it induces the increase of Quantitative Determination of Ergosterol in edible fungus to become by external condition and explores this problem in science
New Century Planned Textbook, there is researcher to find salicylic acid and methyl jasmonate (Methyl jasmonate, MeJA) etc. in succession both at home and abroad
The external source sprinkling processing of inducer can influence Quantitative Determination of Ergosterol in edible fungus, and technology green, it is economical, easy,
It easily promotes, and directly acts on product, it can direct marketing and edible after mushroom picking.
MeJA is a kind of endogenous hormones and signaling molecule being widely present in plant, is capable of selective induction specific gene
Expression, causes a series of physiological changes in vegetable metabolic process, to adjust the accumulation of plant cell secondary metabolite.Mesh
Before, in terms of to MeJA, the research hotspot of physiological action concentrates on its disease resistance and signal transduction in plant.Research has shown that,
MeJA can promote the generation and accumulation of active material in plant and microbial body, and external source MeJA can be planted by excitation defence
The expression of object gene, the activity for adjusting enzyme, the approach such as synthesis of secondary metabolites in inductor, can delayed fruit it is mature into
Journey.These researchs show that MeJA is promoting secondary metabolites generation in plant and microflora organisms to play important work
With, while the research in terms of active material accumulation being promoted in relation to MeJA also to start to become the hot spot that scholar probes into edible fungus
One of, achievement will equally become the theoretical basis and technical support for improving edible fungus nutritive value and functional quality.
Three, summary of the invention
Technical problem
Edible fungus is one of the main path that human body obtains ergosterol, and agaricus bisporus is ergot in numerous edible fungus
Sterol content one kind the most abundant, but its net content level is not still high.Promoting Quantitative Determination of Ergosterol in agaricus bisporus can promote
More high-quality food source is become, for promoting the research of Quantitative Determination of Ergosterol in agaricus bisporus, focuses primarily upon utilize base before this
Because engineering means obtain the bacterial strain of ergosterol high yield, and further promote biosynthesis, but its skill by controlling technological parameter
Art process is cumbersome, still needs to handle by multiprogramming before obtaining matured product, is unable to direct marketing and eats, to not obtain wide
General Technique Popularizing.Currently, in edible fungus cultivation add exogenous inducing agent improve wherein Quantitative Determination of Ergosterol at
To solve the problems, such as this low new angle of its Quantitative Determination of Ergosterol.Through testing and verification, one is sprayed during cultivation of agaricus bisporus
The content for ergosterol of determining the MeJA solution of concentration when can significantly improve bacterial strain maturation in agaricus bisporus, the technical operation is easy,
It is low in cost, easy to spread, after picking can direct marketing and edible, and be suitable for industrialized scale utilization, to produce high ergot
The high-quality agaricus bisporus of sterol content is provided fundamental basis and technical support.
Technical solution
The technical solution adopted by the present invention to solve the technical problems step are as follows:
(1) inducer is prepared: preparing the ethanol-water solution that concentration of alcohol is 20 μ L/mL, it is 100 μ that MeJA, which is made into concentration,
The ethanol-water solution of M is as inducer；
(2) spraying treatment: pouring into full-automatic sprinkling system for inducer, harvests preceding 48~72h to its table in agaricus bisporus
Inducer is uniformly sprayed in face, and sprinkling dosage is 10~25mL/m2；
(3) mushroom is cultivated: after inducer sprinkling processing, being cultivated temperature and is maintained at 22 ± 1 DEG C, humidity is maintained at 90%, plants
Training room is protected from light, and is picked after maturation.
Beneficial effect
During conventional cultivation, agaricus bisporus is when being developed to immature immature button, Quantitative Determination of Ergosterol highest, energy
Reach 10.80mg/g, and is down to 3.87mg/g in the maturity period.On how to stablize and promoted agaricus bisporus maturity period ergosterol
This problem of content is presently mainly solved using biological engineering means, promotes ergot steroid in mushroom by technique for gene engineering
The biosynthesis of alcohol, but related-art process is cumbersome, at high cost, and generalization is poor.In contrast, the present invention is in cultivation of agaricus bisporus
It handling in the process using MeJA and carries out surface sprinkling, Quantitative Determination of Ergosterol is dramatically increased compared with common cultivation kind when its maturation,
The nutritive value and functionality edible quality of agaricus bisporus are substantially increased, while helping to improve agaricus bisporus cell integrity
Can, provided green conducive to the fresh-keeping and transport after adopting how to improve this problem in science of Quantitative Determination of Ergosterol in agaricus bisporus
Color, economy, convenient, the theories integration and solution route easily promoted have been opened up the edible fungus functional nutrient factor and have been strengthened and product
The new approaches of tired research.
Four, Detailed description of the invention
Attached drawing 1 is influence of 100 μM of methyl jasmonate treatments to Quantitative Determination of Ergosterol in agaricus bisporus.
Five, the specific embodiment invented
Method and feature of the invention are described in conjunction with example in detail below, example is served only for explaining this hair
It is bright, it is not intended to limit the scope of the present invention.
Fresh agaricus bisporus used in case study on implementation of the present invention is provided by Jiangsu Tian Feng Biotechnology Co., Ltd.
Influence of the embodiment MeJA processing to Quantitative Determination of Ergosterol in agaricus bisporus is studied
The ethanol-water solution that concentration of alcohol is 20 μ L/mL is prepared, it is water-soluble that MeJA is made into the ethyl alcohol-that concentration is 100 μM
Liquid is as inducer.Inducer is poured into full-automatic sprinkling system, 48h is uniformly sprayed to its surface before agaricus bisporus harvests lures
Agent is led, sprinkling dosage is 20mL/m2As processing group, handled with 20 μ L/mL ethanol solutions as control group.It is picked after 48h,
Every group of grab sample, liquid nitrogen grinding crush, and dehydrated alcohol extraction overnight, carries out HPLC analysis after 0.45 μm of membrane filtration, survey
Determine Quantitative Determination of Ergosterol, and carry out statistical analysis, the results are shown in attached figure 1.
As a result, it has been found that compared with the control group, its content of MeJA processing group is increased to by the 5.49mg/g DW of control group
8.97mg/g DW is equivalent to and improves 1.5 times or more, thus proves its wheat when MeJA can significantly improve agaricus bisporus harvesting
The content of angle sterol.
Claims (2)
1. a kind of method for improving Quantitative Determination of Ergosterol in agaricus bisporus, it is characterised in that: using methyl jasmonate in cultivation
Agaricus bisporus carry out sprinkling processing；Steps are as follows: (1) inducer is prepared: ethanol-water solution is prepared, methyl jasmonate is molten
In ethanol-water solution as inducer；(2) spraying treatment: pouring into full-automatic sprinkling system for inducer, harvests in agaricus bisporus
Preceding 48~72h uniformly sprays inducer to its surface；(3) mushroom is cultivated: after inducer sprinkling processing, being cultivated temperature and is maintained at 22
± 1 DEG C, humidity is maintained at 90%, and cultivating chamber is protected from light, and picks after mature；
Concentration of alcohol in the ethanol-water solution of the step (1) are as follows: 20 μ L/mL；MeJA is dense in the inducer of the step (1)
Degree is 100 μM；
The sprinkling dosage of the step (2) is 10~25mL/m2。
2. the agaricus bisporus that the method according to claim 11 obtains Quantitative Determination of Ergosterol raising.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107347451A (en) * | 2017-08-14 | 2017-11-17 | 佛山推启农业研究院（普通合伙） | A kind of artificial cultivation method for improving hickory chick sterol content |
| CN109526549A (en) * | 2018-12-10 | 2019-03-29 | 瞿瀚鹏 | Agaricus bisporus and its breeding method and application rich in ergosterol |
| CN109566261A (en) * | 2018-12-10 | 2019-04-05 | 南京财经大学 | A method of promoting agaricus bisporus growth and preservation quality |
| CN114766281A (en) * | 2022-05-24 | 2022-07-22 | 西北大学 | Production method of black fungus rich in ergosterol |
Citations (4)
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| JP2000135032A (en) * | 1998-10-30 | 2000-05-16 | Nippon Soda Co Ltd | Agent for growing healthy seedling and healthy seedling growth using the agent |
| CN102047812A (en) * | 2009-10-30 | 2011-05-11 | 李守民 | High-yield cultivation technique of white mushrooms |
| CN104823720A (en) * | 2015-06-04 | 2015-08-12 | 江苏丘陵地区南京农业科学研究所 | Cultivation method of agaricus bisporus |
| CN104920070A (en) * | 2015-06-17 | 2015-09-23 | 江苏省农业科学院宿迁农科所 | Method for increasing yield of agaricus bisporus |
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- 2016-04-08 CN CN201610223425.8A patent/CN105706741B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000135032A (en) * | 1998-10-30 | 2000-05-16 | Nippon Soda Co Ltd | Agent for growing healthy seedling and healthy seedling growth using the agent |
| CN102047812A (en) * | 2009-10-30 | 2011-05-11 | 李守民 | High-yield cultivation technique of white mushrooms |
| CN104823720A (en) * | 2015-06-04 | 2015-08-12 | 江苏丘陵地区南京农业科学研究所 | Cultivation method of agaricus bisporus |
| CN104920070A (en) * | 2015-06-17 | 2015-09-23 | 江苏省农业科学院宿迁农科所 | Method for increasing yield of agaricus bisporus |
Non-Patent Citations (1)
| Title |
|---|
| MeJA对猴头5L0433菌株麦角甾醇合成的影响及消减文库的ESTs分析;李迎雪;《中国优秀硕士学位论文全文数据库 农业科技辑》;20111115(第11期);第D048-35页 |
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