CN1338510A - Artificial culture method of north cordyceps sporophores and its living product - Google Patents
Artificial culture method of north cordyceps sporophores and its living product Download PDFInfo
- Publication number
- CN1338510A CN1338510A CN 01126620 CN01126620A CN1338510A CN 1338510 A CN1338510 A CN 1338510A CN 01126620 CN01126620 CN 01126620 CN 01126620 A CN01126620 A CN 01126620A CN 1338510 A CN1338510 A CN 1338510A
- Authority
- CN
- China
- Prior art keywords
- cordyceps
- rejuvenation
- sporophore
- tieback
- caterpillar fungus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
An artificial culture method for north cordyceps sporophores includes such steps as selecting excellent strains (cordyceps body), separating, screening, purifying to obtain high-yield spawns, adding them to culture medium in pp bottles, culturing sporophores under light radiation and temp contron condition, inoculating into living silkworm chrysalis, and rejuvenescent culture to obtain fresh living cordyceps product. Its advantages include big body (3-4 mm X 4.5-8.5cm), higher contents of cordyceptic extract (polyose) and SOD, and low cost.
Description
Technical field:
The present invention relates to a kind of artificial breeding technique of Cordyceps sinensis, particularly disclose the fresh living cordyceps product of a kind of cordyceps militaris fruit body artificial culturing method and large-scale production thereof.
Background technology:
Cordyceps sinensis is the distinctive rare medicinal herbs of China, with pilose antler, the same three big treasures of classifying Chinese medicine as of genseng, traditional Chinese medicine thinks that Cordyceps sinensis has tonifying deficiency, beneficial vital essence, protects effects such as lung, kidney-nourishing, and medical research is found to also have calmness, hypoxia tolerance, anti-inflammatory, step-down, improved effects such as myocardial ischemia and raising immune function of human body in recent years.For a long time, domestic medicinal Chinese caterpillar fungus is picked up from wild, and along with exceeding ecotope day of Chinese caterpillar fungus life suffers artificial destruction, the long naturally amount of worm source resource is lower than mankind's amount of excavating, and it is exhausted that the wild Chinese caterpillar fungus order becomes; The artificial culture of Chinese caterpillar fungus rises; its greatest difficulty of artificial culture Cordyceps militaris (L.) Link. is spawn degeneration; so domestic and international at present precedent of still under the prerequisite of sporophore shape normal growth, accomplishing large-scale production; rest on the experimental phase for a long time; even there are extremely indivedual units that certain turnout is arranged; but from sporophore shape, all belong to deformity, unordered growing way.And with fresh living cordyceps as the finished product; its not only requirement can accomplish mass-producing, batch production production; and the also necessary growing way of the profile that requires its sporophore growth is well-balanced, consistence is good, and the whole process technology that fundamentally improves the artificial culture cordyceps militaris fruit body does not appear in the newspapers.
Summary of the invention:
The technical problem to be solved in the present invention is the problem that spawn degeneration still can not be accomplished large-scale production that produces in the artificial culture Cordyceps militaris (L.) Link.; by the artificial fresh living cordyceps of cultivating of the present invention; drop into low, output is high and the effect of wild Chinese caterpillar fungus is arranged; and cost is significantly less than natural cs, thereby has overcome the contradiction that the exhausted day by day social needs amount of natural cs resource improves day by day.
Technical scheme of the present invention promptly is to adopt a kind of utilization rejuvenation of spawn technology; promptly pass through the polypide tieback of Cordyceps militaris (L.) Link.; thereby prevent and delay spawn degeneration; make sporophore reach high yield; the method of formation anniversary large-scale production; it is characterized in that wild Chinese caterpillar fungus bacterium mycelium tieback is carried out rejuvenation in the silkworm chrysalis body; getting Cordyceps after its rejuvenation then is inoculated in the substratum in the special-purpose PP bottle; be placed on the culturing rack and cultivate; room temp is controlled at 18 °～22 ℃; under no optical condition, cultivate a week earlier; after move under the scattered light and to continue to support 28～32 days; produce sporophore, the ripe final fresh living cordyceps product that forms of sporophore after two months, its processing technology routine is as follows:
Method by biotechnology of the present invention, the fresh and alive Cordyceps sinensis of artificial culture, reach nutrition and pharmaceutical use is close to or higher than natural cordyceps, and make cordycepin and SOD (bifid super-oxide enzyme) content higher, cost is low, effect is high than natural cordyceps (Chinese Chinese caterpillar fungus), cheap, be life nutritious prod, the health invigorant that the numerous common people love, promote that the numerous common people's is healthy.
Description of drawings:
Accompanying drawing fresh living cordyceps artificial culture of the present invention processing technology routine skeleton diagram.
Embodiment:
With reference to the accompanying drawings, the strain excellent polypide is gathered, separate, cultivate, screening, get the different sites of its Chinese caterpillar fungus, the methods such as suitable culture condition and different strain pairing of seeking have obtained good original silkworm egg, silk injects the PP bottle that is equipped with substratum on the Cordyceps that inoculation back rejuvenation is cultivated, under aforesaid illumination and temperature control condition, cultivate sporophore, generate fresh living cordyceps, the preferred Philosamia cynthia chrysalis of the silkworm chrysalis live body body that the inoculation rejuvenation is cultivated, not only can improve fruiting body yield, and pass through rejuvenation of spawn, be of great importance to preventing and delaying spawn degeneration, with fresh living cordyceps in the PP bottle is the finished product, thisly makes Chinese caterpillar fungus have more activity with fresh and alive form, has broken through nutritious prod, healthcare products are often with oral liquid, capsular traditional mode.
Beneficial effect of the present invention:
1) manually cultivation method of the present invention obtains fresh and alive Cordyceps militaris fructification national departments concerned mensuration, and it effectively becomes Part is close to or higher than natural cordyceps (cordyceps sinensis), and the effect of wild Chinese caterpillar fungus is arranged, and Cordyceps sinensis polysaccharide Content is higher than 4.5 times of natural cses, and SOD content 3000u/g is higher than surplus the natural cordyceps 80 times, Still contain the one-tenth such as abundant cordycepic acid, bifid superoxides energy, amino acid, various trace elements, vitamin Part, the present invention obtains product and has reached the world from the high-load of cordycepin, Cordyceps sinensis polysaccharide, SOD and intake earlier Flat.
2) utilization rejuvenation of spawn technology of the present invention, i.e. polypide tieback by northern Chinese caterpillar Fungus, thus prevent and prolong Slow spawn degeneration makes fructification reach high yield, forms the method for anniversary large-scale production, the rejuvenation of spawn skill Art has played positive role for improving fructification output and quality, is preventing and to continue spawn degeneration technical Obtained breakthrough.
3) the product input of the present invention's acquisition is low, output is high, its nutrition and medicinal value height, and its selling price is greatly Be lower than natural cs, easily accepted by numerous people, have potential market so tool at home, abroad Very high economic benefit and social benefit are arranged.
Claims (3)
1; a kind of cordyceps militaris fruit body artificial culturing method; it is a utilization rejuvenation of spawn technology; promptly pass through the polypide tieback of Cordyceps militaris (L.) Link.; thereby prevent and delay spawn degeneration; make sporophore reach high yield; the method of formation anniversary large-scale production; it is characterized in that wild Chinese caterpillar fungus bacterium mycelium tieback is carried out rejuvenation in the silkworm chrysalis body; getting Cordyceps after its rejuvenation then is inoculated in the substratum in the special-purpose PP bottle; be placed on the culturing rack and cultivate; room temp is controlled at 18～22 ℃; under no optical condition, cultivate a week earlier, after move to and continued the generation sporophore scattered light under foster 28～32 days; the ripe final fresh living cordyceps product that forms of sporophore after two months, its processing technology routine is as follows:
Polypide collection, separation, cultivation, screening, inoculation, substratum, sporophore is cultivated, detects, packs, puts in storage, is dispatched from the factory under illumination, temperature control condition; Wild Chinese caterpillar fungus bacterium tieback to the silkworm chrysalis worm is in vivo drawn rejuvenation cultivation Cordyceps and is produced in addition.
2, cordyceps militaris fruit body artificial culturing method according to claim 1 is characterized in that the wild Chinese caterpillar fungus bacterium mycelium tieback of described rejuvenation of spawn technology in vivo carries out rejuvenation to the Philosamia cynthia pupa.
3, a kind ofly it is characterized in that by the multiple grass seed entity fresh living cordyceps product of claim 1 or 2 artificial culturing methods north winter worm the fresh living cordyceps stroma is orange red in the PP bottle, cylindrical, long 4.5～8.5cm, diameter 3～4mm, head is cylindrical to club shape, the handle column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011266201A CN1156569C (en) | 2001-09-04 | 2001-09-04 | Artificial culture method of north cordyceps sporophores and its living product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011266201A CN1156569C (en) | 2001-09-04 | 2001-09-04 | Artificial culture method of north cordyceps sporophores and its living product |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1338510A true CN1338510A (en) | 2002-03-06 |
| CN1156569C CN1156569C (en) | 2004-07-07 |
Family
ID=4666631
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011266201A Expired - Fee Related CN1156569C (en) | 2001-09-04 | 2001-09-04 | Artificial culture method of north cordyceps sporophores and its living product |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1156569C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1312271C (en) * | 2004-02-27 | 2007-04-25 | 俞永信 | Method of whole manual culture for chinese caterpillar fungus |
| CN100402639C (en) * | 2006-12-29 | 2008-07-16 | 上海市农业科学院 | Northern aweto strain and its selection breeding method |
| CN102754562A (en) * | 2011-04-24 | 2012-10-31 | 陈大宫 | Artificial cultivation technology for Cordyceps iaponica |
| CN103404368A (en) * | 2013-07-12 | 2013-11-27 | 重庆市中药研究院 | Rejuvenation culture method for artificial breeding of cordyceps militaris strain |
| CN103483038A (en) * | 2013-09-05 | 2014-01-01 | 东方中科生命科学有限责任公司 | Preparation and culture method of Cordyceps culture medium, and application thereof |
| CN105706742A (en) * | 2016-04-18 | 2016-06-29 | 程致远 | Culture method of cordyceps militaris shells and shell cordyceps militaris |
| CN105746173A (en) * | 2016-03-01 | 2016-07-13 | 中船重工河北清洗机有限公司 | Intelligent cultivation and production method and intelligent cultivation and production device for cordyceps sinensis |
| CN108925370A (en) * | 2018-05-15 | 2018-12-04 | 复旦大学 | High yield Cordceps militaris cultivates lighting system and breeding method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102037856B (en) * | 2009-10-16 | 2012-06-20 | 北京农业生物技术研究中心 | Simple cordyceps militaris strain rejuvenation method |
-
2001
- 2001-09-04 CN CNB011266201A patent/CN1156569C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1312271C (en) * | 2004-02-27 | 2007-04-25 | 俞永信 | Method of whole manual culture for chinese caterpillar fungus |
| CN100402639C (en) * | 2006-12-29 | 2008-07-16 | 上海市农业科学院 | Northern aweto strain and its selection breeding method |
| CN102754562A (en) * | 2011-04-24 | 2012-10-31 | 陈大宫 | Artificial cultivation technology for Cordyceps iaponica |
| CN103404368A (en) * | 2013-07-12 | 2013-11-27 | 重庆市中药研究院 | Rejuvenation culture method for artificial breeding of cordyceps militaris strain |
| WO2015003547A1 (en) * | 2013-07-12 | 2015-01-15 | 重庆市中药研究院 | Culture method for natural species rejuvenation of artificially bred cordyceps strains |
| CN103483038A (en) * | 2013-09-05 | 2014-01-01 | 东方中科生命科学有限责任公司 | Preparation and culture method of Cordyceps culture medium, and application thereof |
| CN103483038B (en) * | 2013-09-05 | 2015-06-24 | 东方中科生命科学有限责任公司 | Preparation and culture method of Cordyceps culture medium, and application thereof |
| CN105746173A (en) * | 2016-03-01 | 2016-07-13 | 中船重工河北清洗机有限公司 | Intelligent cultivation and production method and intelligent cultivation and production device for cordyceps sinensis |
| CN105706742A (en) * | 2016-04-18 | 2016-06-29 | 程致远 | Culture method of cordyceps militaris shells and shell cordyceps militaris |
| CN105706742B (en) * | 2016-04-18 | 2018-10-02 | 程致远 | A kind of cultural method of cordyceps sinensis shellfish and shellfish cordyceps sinensis |
| CN108925370A (en) * | 2018-05-15 | 2018-12-04 | 复旦大学 | High yield Cordceps militaris cultivates lighting system and breeding method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1156569C (en) | 2004-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106544279B (en) | A kind of hickory chick cultivation strain and its induction and cultural method | |
| CN104145719B (en) | A kind of Cordyceps fungus mycelium fermentation production method | |
| CN102440149B (en) | Method for breeding cordyceps militaris by utilizing silkworm larvae | |
| CN1029382C (en) | Method for artificially cultivating north Chinese caterpiller fungus daughter vaccine | |
| CN102763560B (en) | Cordyceps sobolifera fruiting body with cicada larva as host and culture method thereof | |
| CN101215527A (en) | Method for cultivating silkworm chrysalis Cordyceps sinensis | |
| CN102612985A (en) | Production technology for cordyceps militaris mycelium | |
| CN103270887B (en) | Silkworm chrysalis northern Chinese caterpillar Fungus industrial cultivation technique | |
| CN105660191B (en) | A kind of cultural method of crow sesame fructification | |
| CN104350942A (en) | Method for preparing cordyceps mycelia through mulberry twig powder and silkworm chrysalis powder | |
| CN100575475C (en) | The method of wheat cultivation Cordyceps militaris | |
| CN1224695C (en) | New technology of culturing cordyceps sinensis using insect | |
| CN109644779A (en) | A method of silkworm grass is cultivated using cordyceps inoculation 5 instar larvae of castor silkworm | |
| CN1156569C (en) | Artificial culture method of north cordyceps sporophores and its living product | |
| CN101045904A (en) | Aweto sporophore culturing process | |
| CN109105153B (en) | Efficient cultivation method of tussah cordyceps militaris | |
| CN105557312B (en) | A method of using Yellow meal worm larva as carrier culture worm grass product | |
| CN101142897A (en) | Radix pseudostell adventitious root inducing and tissue culturing method | |
| CN102613007A (en) | Pollution-free culture method for cordyce militaris | |
| CN105493899B (en) | A method of using yellow meal worm worm pupa as carrier culture worm grass product | |
| CN103392513B (en) | Ferment of aweto mycelia and application of ferment | |
| CN104521559A (en) | Factory-like bottle-cultivation hypsizygus marmoreus growth stage carbon dioxide concentration control method | |
| CN103461005A (en) | Method for preparing cordyceps sinensis block by germinant wheat | |
| CN1082807A (en) | The method of artificially culturing north Chinese caterpillar fungus | |
| CN115104477A (en) | Indoor industrial cultivation method for organic morchella |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20040707 Termination date: 20100904 |