CN113875659A - Ecological breeding and propagation method of bait-feeding fairy shrimp for increasing yield of crab seedlings - Google Patents
Ecological breeding and propagation method of bait-feeding fairy shrimp for increasing yield of crab seedlings Download PDFInfo
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- 241000595940 Notostraca Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000009395 breeding Methods 0.000 title claims abstract description 18
- 230000001488 breeding effect Effects 0.000 title claims abstract description 17
- 230000001902 propagating effect Effects 0.000 claims abstract description 3
- 235000013601 eggs Nutrition 0.000 claims description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 238000011534 incubation Methods 0.000 claims description 23
- 238000003756 stirring Methods 0.000 claims description 19
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 16
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 230000035764 nutrition Effects 0.000 claims description 11
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- 239000003054 catalyst Substances 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 9
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 8
- 230000012447 hatching Effects 0.000 claims description 8
- 238000012216 screening Methods 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 8
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 8
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 8
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 8
- 235000010288 sodium nitrite Nutrition 0.000 claims description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 5
- 229930003268 Vitamin C Natural products 0.000 claims description 5
- 235000019154 vitamin C Nutrition 0.000 claims description 5
- 239000011718 vitamin C Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 239000004343 Calcium peroxide Substances 0.000 claims description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 241001465382 Physalis alkekengi Species 0.000 claims description 4
- 241000607598 Vibrio Species 0.000 claims description 4
- 206010047400 Vibrio infections Diseases 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- LHJQIRIGXXHNLA-UHFFFAOYSA-N calcium peroxide Chemical compound [Ca+2].[O-][O-] LHJQIRIGXXHNLA-UHFFFAOYSA-N 0.000 claims description 4
- 235000019402 calcium peroxide Nutrition 0.000 claims description 4
- 239000000460 chlorine Substances 0.000 claims description 4
- 229910052801 chlorine Inorganic materials 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 230000000249 desinfective effect Effects 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000011049 filling Methods 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 239000013505 freshwater Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 238000006213 oxygenation reaction Methods 0.000 claims description 4
- 239000002244 precipitate Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 238000005728 strengthening Methods 0.000 claims description 4
- 230000001502 supplementing effect Effects 0.000 claims description 4
- 241001247197 Cephalocarida Species 0.000 claims 3
- 231100000989 no adverse effect Toxicity 0.000 claims 1
- 241000238557 Decapoda Species 0.000 abstract description 24
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000036039 immunity Effects 0.000 abstract description 4
- 229930003231 vitamin Natural products 0.000 abstract description 4
- 235000013343 vitamin Nutrition 0.000 abstract description 4
- 239000011782 vitamin Substances 0.000 abstract description 4
- 229940088594 vitamin Drugs 0.000 abstract description 4
- 206010006956 Calcium deficiency Diseases 0.000 abstract description 2
- 230000008901 benefit Effects 0.000 abstract description 2
- 239000013589 supplement Substances 0.000 abstract description 2
- 230000009466 transformation Effects 0.000 abstract description 2
- 241000238631 Hexapoda Species 0.000 abstract 2
- 150000003722 vitamin derivatives Chemical class 0.000 abstract 2
- 206010057249 Phagocytosis Diseases 0.000 abstract 1
- 230000008782 phagocytosis Effects 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 8
- 241000700141 Rotifera Species 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241000733943 Hapalogaster mertensii Species 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000009931 harmful effect Effects 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000000366 juvenile effect Effects 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 206010016260 Fatty acid deficiency Diseases 0.000 description 1
- 241000167880 Hirundinidae Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940069978 calcium supplement Drugs 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biomedical Technology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an ecological breeding and propagating method of a bait Fengning insect for increasing the yield of crab seedlings, wherein shells taken off by the Fengning insect contain rich Ca2+The method can effectively supplement the problem of calcium deficiency of the young crabs, the fairy shrimp bred by the method is eaten by crab seedlings, the immunity of the crab seedlings is improved, the survival rate is improved, the quality of the crabs is good, the fairy shrimp can be bred and bred in a large scale, the operation is simple and convenient, and the technical problem of the fairy shrimp breeding and breeding is solved. The vitamin is administered to fairy shrimp by utilizing the phagocytosis characteristic of fairy shrimp, and then fed to young crab, so that most of vitamin can be directly absorbed by young crabThe secondary transformation is avoided, after the crab larvae eat high-quality fairy shrimp, the physique is enhanced, and the survival rate of the larvae is improved from 50-55 percent to 80-90 percent, so that the breeding benefit of the crabs is increased.
Description
Technical Field
The invention relates to a breeding method of crab larva bait, in particular to an ecological breeding and propagation method of bait Toyota kunmenbacea for increasing the yield of crab seedlings.
Background
In China, the crab has fresh and tender meat quality, is just like to become a popular and pleasant dining table taste, particularly the hairy crabs such as the Yangcheng lake hairy crab, the Gucheng lake hairy crab, the Yangcheng lake hairy crab and the like, and enjoys popularity in China. However, the yield of the crabs is not high all the time, death due to diseases is the main reason, and death of the crabs is the direct reason that the yield is difficult to improve, wherein the crabs eat less food when the crabs are peeled in the 1 stage and are transited to the 2 stage, so that the resistance is poor, the death phenomenon is easy to occur, the immunity of the crabs in the period is enhanced, and the crabs need to be fed with the food with rich nutrition. Some crabs are easy to have the black shell disease, most of the disease occurs in intensive culture ponds in high-temperature seasons in summer, when the feed intake is large and the crabs grow vigorously, the feed is not full of nutrition, the shells of the crabs are calcified due to lack of calcium and vitamin C, the inner new shells of the crabs are lack of calcium, and the crabs die due to abnormal shelling. The invention patent 201510755297.7 discloses a method for breeding crabs by using rotifers, which is an ideal aquaculture bait for natural rotifers and fairy bugs, but the natural rotifers and fairy bugs have limited resources, and the artificially bred rotifers have the phenomenon of fatty acid deficiency and need to be subjected to nutrition enrichment. The rotifer takes food in a chewing manner, directly digests and absorbs the nutrition enhancer, and then the nutrition enhancer is absorbed by the crab, and is transformed and absorbed. The fairy shrimp swallows slowly to eat, and the nutrition enhancer is fed before feeding the juvenile crabs, so that the nutrition enhancer is directly absorbed by the juvenile crabs, and the effect is better than that of rotifers. The fairy shrimp is the bait of natural crab larvae, so the fairy shrimp suitable for the bait of the crab larvae is cultured, the immunity and the growth of the crab larvae are further improved, the survival rate and the quality of the crab larvae are improved, and the research prospect of expanding the culture yield is wide. In order to solve the problem, technicians of scientific research institutions and enterprises are continuously exploring and researching to hope to find a fairy shrimp seedling raising method with higher efficiency, and although certain progress is made, the technical problem which is not overcome in the actual application still exists.
Disclosure of Invention
The invention aims to overcome the defects and provide an ecological breeding and propagation method of the bait Toyota kuwana which can increase the yield of crab seedlings. The shell of fairy shrimp contains rich Ca2+The method can effectively supplement the problem of calcium deficiency of the young crabs, the fairy shrimp bred by the method is eaten by crab seedlings, the immunity of the crab seedlings is improved, the survival rate is improved, the quality of the crabs is good, the fairy shrimp can be bred and bred in a large scale, the operation is simple and convenient, and the technical problem of the fairy shrimp breeding and breeding is solved.
The technical scheme adopted by the invention for solving the technical problems is as follows:
an ecological breeding and propagating method of bait Toyota kummensis for increasing the yield of crab seedlings comprises the following steps:
step 1: screening fairy shrimp eggs, screening eggs which are shriveled and can not be hatched, soaking the fairy shrimp eggs in sodium hypochlorite (5 ppm) for 15-20min before hatching, and cleaning residual sodium hypochlorite in the eggs with fresh water after soaking.
Step 2: disinfecting an incubation barrel, injecting water for 24 hours in advance, using trichloro (10 ppm for disinfection and aerating for 12 hours), heating the water to 26-30 ℃ after injecting water, filling an air chamber in the incubation barrel, wherein the more the air chamber is, the better the air chamber is, preventing the eggs from sinking, sprinkling sodium bicarbonate (no harmful effect is found when the sodium bicarbonate is excessive), and after aerating for 1 hour, sprinkling EDTA (3 ppm), wherein the eggs can be released (the smell of water before releasing the eggs has the taste of chlorine, and the eggs can be released only after aerating).
And step 3: and (2) placing the worm eggs treated in the step (1) into the incubation barrel treated in the step (2), stirring once every 2 hours until eggs with lanterns appear, stopping stirring to prevent the worm eggs from sinking to the bottom, wherein sufficient oxygen is contained in the incubation barrel, supplementing a light source (light source 1000 and 2000 luxes) by an incandescent lamp when the illumination is insufficient, splashing a catalyst (50 g-70 g/cubic meter) after 2.5 hours, stirring immediately after splashing to prevent the catalyst and the worm eggs from sinking to the bottom, and keeping the temperature at 28-30 ℃ in the incubation process.
And 4, step 4: and (3) strengthening nutrition, namely transferring the fairy shrimp nauplii in the B end to a fairy shrimp culturing pond, keeping the water quality clear, and regularly feeding baits and vitamin C.
And 5: after the incubation is finished, only collecting the hatching fluid in the first 30 minutes, stopping oxygenation, shading with a black film, allowing the fairy shrimp to sink, floating the empty shell, and separating.
And (3) sterilizing the empty shell, wherein the empty shell is provided with a large amount of vibrios and filamentous bacteria, stirring the empty shell in a 15% sodium nitrite solution for 2 hours, and cleaning the empty shell for 3 times by using clear water to remove residual sodium nitrite.
The removal of Fe in the hollow shell2+Comprises the following steps: adding the empty shell into 10% sodium hydroxide solution, stirring and reacting to remove Fe in the empty shell2+Taking out and cleaning the residual precipitate, and dryingAnd (5) drying.
The catalyst is as follows: 65-70% of calcium peroxide.
The invention has the beneficial effects that:
1. fe in the shell of fairy shrimp2+Removed as calcium supplement substance of the young crab, saves cost and improves utilization rate.
2. Vitamins are taken by the fairy shrimp by utilizing the phagocytic property of the fairy shrimp and then fed to the young crabs, so that most of the vitamins are directly absorbed by the young crabs, secondary transformation is avoided, after the young crabs ingest high-quality fairy shrimp, the physique is enhanced, and the survival rate of the young crabs is improved from 50-55 percent to 80-90 percent, thereby increasing the culture benefit of the young crabs.
3. The fairy shrimp can be bred and propagated in large scale, is simple and convenient to operate, and solves the technical problem of fairy shrimp breeding and propagation.
Detailed Description
Example one
Step 1: screening fairy shrimp eggs, screening eggs which are shriveled and can not be hatched, soaking the fairy shrimp eggs in sodium hypochlorite (5 ppm) for 15-20min before hatching, and cleaning residual sodium hypochlorite in the eggs with fresh water after soaking.
Step 2: disinfecting an incubation barrel, using trichloro (10 ppm for disinfection and aerating for 12 hours) 24 hours before water injection and water extraction, heating the water to 26-30 ℃ after the water injection, filling a gas chamber in the incubation barrel, wherein the more the water is, the better the gas is, preventing the eggs from sinking, sprinkling sodium bicarbonate (no harmful effect is found when the sodium bicarbonate is excessive), and sprinkling EDTA (3 ppm) after the gas is aerated for 1 hour, wherein the eggs can be released (the smell of the water before the eggs are released is the smell of chlorine, and the eggs can be released only after the gas is aerated).
And step 3: and (2) placing the worm eggs treated in the step (1) into the incubation barrel treated in the step (2), stirring once every 2 hours until eggs with lanterns appear, stopping stirring to prevent the worm eggs from sinking to the bottom, wherein sufficient oxygen is contained in the incubation barrel, supplementing a light source (light source 1000 luxes) by an incandescent lamp when the illumination is insufficient, sprinkling a catalyst (50 g/cubic meter) after 2.5 hours, stirring immediately after sprinkling to prevent the catalyst and the worm eggs from sinking to the bottom, and keeping the temperature at 28-30 ℃ in the incubation process.
And 4, step 4: and (3) strengthening nutrition, namely transferring the fairy shrimp nauplii in the B end to a fairy shrimp culturing pond, keeping the water quality clear, and regularly feeding baits and vitamin C.
And 5: after the incubation is finished, only collecting the hatching fluid in the first 30 minutes, stopping oxygenation, shading with a black film, allowing the fairy shrimp to sink, floating the empty shell, and separating.
And (3) sterilizing the empty shell, wherein the empty shell is provided with a large amount of vibrios and filamentous bacteria, stirring the empty shell in a 15% sodium nitrite solution for 2 hours, and cleaning the empty shell for 3 times by using clear water to remove residual sodium nitrite.
The removal of Fe in the hollow shell2+Comprises the following steps: adding the empty shell into 10% sodium hydroxide solution, stirring and reacting to remove Fe in the empty shell2+And fishing out the residual precipitate, and drying.
The catalyst is as follows: 65% of calcium peroxide.
Example two
Step 1: screening fairy shrimp eggs, screening eggs which are shriveled and can not be hatched, soaking the fairy shrimp eggs in sodium hypochlorite (5 ppm) for 15-20min before hatching, and cleaning residual sodium hypochlorite in the eggs with fresh water after soaking.
Step 2: disinfecting an incubation barrel, using trichloro (10 ppm for disinfection and aerating for 12 hours) 24 hours before water injection and water extraction, heating the water to 26-30 ℃ after the water injection, filling a gas chamber in the incubation barrel, wherein the more the water is, the better the gas is, preventing the eggs from sinking, sprinkling sodium bicarbonate (no harmful effect is found when the sodium bicarbonate is excessive), and sprinkling EDTA (3 ppm) after the gas is aerated for 1 hour, wherein the eggs can be released (the smell of the water before the eggs are released is the smell of chlorine, and the eggs can be released only after the gas is aerated).
And step 3: and (2) placing the worm eggs treated in the step (1) into the incubation barrel treated in the step (2), stirring once every 2 hours until eggs with lanterns appear, stopping stirring to prevent the worm eggs from sinking to the bottom, wherein sufficient oxygen is contained in the incubation barrel, supplementing a light source (light source 2000 lux) by an incandescent lamp when the illumination is insufficient, sprinkling a catalyst (70 g/cubic meter) after 2.5 hours, stirring immediately after sprinkling to prevent the catalyst and the worm eggs from sinking to the bottom, and keeping the temperature at 28-30 ℃ in the incubation process.
And 4, step 4: and (3) strengthening nutrition, namely transferring the fairy shrimp nauplii in the B end to a fairy shrimp culturing pond, keeping the water quality clear, and regularly feeding baits and vitamin C.
And 5: after the incubation is finished, only collecting the hatching fluid in the first 30 minutes, stopping oxygenation, shading with a black film, allowing the fairy shrimp to sink, floating the empty shell, and separating.
And (3) sterilizing the empty shell, wherein the empty shell is provided with a large amount of vibrios and filamentous bacteria, stirring the empty shell in a 15% sodium nitrite solution for 2 hours, and cleaning the empty shell for 3 times by using clear water to remove residual sodium nitrite.
The removal of Fe in the hollow shell2+Comprises the following steps: adding the empty shell into 10% sodium hydroxide solution, stirring and reacting to remove Fe in the empty shell2+And fishing out the residual precipitate, and drying.
The catalyst is as follows: 70% of calcium peroxide.
Claims (4)
1. An ecological breeding and propagating method of bait Toyota kummensis for increasing the yield of crab seedlings comprises the following steps:
step 1: screening fairy shrimp eggs, screening eggs which are shriveled and can not be hatched, soaking the fairy shrimp eggs in sodium hypochlorite (5 ppm) for 15-20min before hatching, and cleaning residual sodium hypochlorite in the eggs with fresh water after soaking;
step 2: disinfecting an incubation barrel, injecting water for 24 hours in advance, using trichloro (10 ppm for disinfection and aerating for 12 hours), heating the water to 26-30 ℃ after injecting water, filling an air chamber in the incubation barrel, wherein the more the water is, the better the air chamber is, preventing the eggs from sinking, sprinkling sodium bicarbonate (no adverse effect is found when the sodium bicarbonate is excessive), and after aerating for 1 hour, sprinkling EDTA (3 ppm), wherein the eggs can be released (the smell of water before releasing the eggs is that the chlorine is available, and the eggs can be released only after aerating is available);
and step 3: placing the worm eggs treated in the step 1 into the incubation barrel treated in the step 2, stirring once every 2h until eggs with lanterns appear, stopping stirring to prevent the worm eggs from sinking to the bottom, wherein sufficient oxygen is contained in the incubation barrel, supplementing a light source (light source 1000-;
and 4, step 4: strengthening nutrition, namely transferring the fairy shrimp nauplii at the B end to a fairy shrimp culturing pond, keeping the water clear, and regularly feeding baits and vitamin C;
and 5: after the incubation is finished, only collecting the hatching fluid in the first 30 minutes, stopping oxygenation, shading with a black film, allowing the fairy shrimp to sink, floating the empty shell, and separating.
2. The ecological breeding and propagation method of the bait artemia cystokiniana for increasing the yield of the crab seedlings according to the claim 1, which is characterized in that: and (3) sterilizing the empty shell, wherein the empty shell is provided with a large amount of vibrios and filamentous bacteria, stirring the empty shell in a 15% sodium nitrite solution for 2 hours, and cleaning the empty shell for 3 times by using clear water to remove residual sodium nitrite.
3. The ecological breeding and propagation method of the bait artemia cystokiniana for increasing the yield of the crab seedlings according to the claim 1, which is characterized in that: the removal of Fe in the hollow shell2+Comprises the following steps: adding the empty shell into 10% sodium hydroxide solution, stirring and reacting to remove Fe in the empty shell2+And fishing out the residual precipitate, and drying.
4. The ecological breeding and propagation method of the bait artemia cystokiniana for increasing the yield of the crab seedlings according to the claim 1, which is characterized in that: the catalyst is as follows: 65-70% of calcium peroxide.
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