CN113874498A - 一种热稳定性葡萄糖氧化酶 - Google Patents
一种热稳定性葡萄糖氧化酶 Download PDFInfo
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- CN113874498A CN113874498A CN202080039321.6A CN202080039321A CN113874498A CN 113874498 A CN113874498 A CN 113874498A CN 202080039321 A CN202080039321 A CN 202080039321A CN 113874498 A CN113874498 A CN 113874498A
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- glucose oxidase
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Abstract
提供了一种通过在野生型黑曲霉葡萄糖氧化酶或突变型黑曲霉葡萄糖氧化酶的氨基酸序列中引入至少一对二硫键获得的热稳定性葡萄糖氧化酶。该葡萄糖氧化酶适合应用于食品、化工、医药、农业和饲料领域中。
Description
PCT国内申请，说明书已公开。
Claims (27)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910469009X | 2019-05-31 | ||
| CN201910469009 | 2019-05-31 | ||
| PCT/CN2020/093205 WO2020239064A1 (zh) | 2019-05-31 | 2020-05-29 | 一种热稳定性葡萄糖氧化酶 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113874498A true CN113874498A (zh) | 2021-12-31 |
Family
ID=73553508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080039321.6A Pending CN113874498A (zh) | 2019-05-31 | 2020-05-29 | 一种热稳定性葡萄糖氧化酶 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20220220454A1 (zh) |
| EP (1) | EP3978602A4 (zh) |
| CN (1) | CN113874498A (zh) |
| WO (1) | WO2020239064A1 (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114736879A (zh) * | 2022-06-09 | 2022-07-12 | 中国农业科学院北京畜牧兽医研究所 | 热稳定性改善的葡萄糖氧化酶GoxM10突变体E361P及其衍生突变体和应用 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| CN112760299B (zh) * | 2021-02-06 | 2022-06-10 | 江南大学 | 热稳定性提高的葡萄糖氧化酶突变体及其编码基因和应用 |
Citations (5)
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|---|---|---|---|---|
| CN1238009A (zh) * | 1996-07-24 | 1999-12-08 | 衣阿华州立大学研究基金会股份有限公司 | 提高葡糖淀粉酶pH最适点、底物专一性和热稳定性的蛋白质工程方法 |
| US20160002609A1 (en) * | 2013-01-28 | 2016-01-07 | Roche Diabetes Care, Inc. | Glucose Oxidase Variants, As Well As Compositions, Devices, Kits and Uses Thereof |
| CN108251389A (zh) * | 2017-08-18 | 2018-07-06 | 青岛蔚蓝生物集团有限公司 | 一种耐热性提高的葡萄糖氧化酶突变体 |
| US10233430B1 (en) * | 2017-10-13 | 2019-03-19 | Dongguan Apac Biotechnology Co.,Ltd. | Glucose oxidase having improved thermostability |
| CN109666657A (zh) * | 2017-10-13 | 2019-04-23 | 东莞泛亚太生物科技有限公司 | 提升耐热性的葡萄糖氧化酶 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5266688A (en) * | 1988-06-21 | 1993-11-30 | Chiron Corporation | Polynucleotide sequence for production of glucose oxidase in recombinant systems |
| US6537792B1 (en) * | 1996-07-24 | 2003-03-25 | Iowa State University | Protein engineering of glucoamylase to increase pH optimum, substrate specificity and thermostability |
| EP2796547B1 (en) * | 2013-04-24 | 2016-09-14 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Novel glucose oxidase variants |
| CN108795891B (zh) * | 2017-04-26 | 2021-03-26 | 中国农业科学院北京畜牧兽医研究所 | 一种葡萄糖氧化酶CnGODA及其基因与应用 |
| CN107858338B (zh) * | 2017-11-29 | 2020-05-15 | 华南农业大学 | 一种组合二硫键的耐热突变脂肪酶及其制备方法与应用 |
| CN108913675B (zh) * | 2018-07-09 | 2020-05-22 | 华南理工大学 | 一种热稳定性提高的脂肪酶突变体及其应用 |
| CN109706137B (zh) * | 2019-01-21 | 2021-02-19 | 天津科技大学 | 一种通过增加二硫键提高肝素酶i热稳定性的突变体及制备方法 |
-
2020
- 2020-05-29 WO PCT/CN2020/093205 patent/WO2020239064A1/zh unknown
- 2020-05-29 EP EP20814947.6A patent/EP3978602A4/en active Pending
- 2020-05-29 CN CN202080039321.6A patent/CN113874498A/zh active Pending
- 2020-05-29 US US17/615,342 patent/US20220220454A1/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1238009A (zh) * | 1996-07-24 | 1999-12-08 | 衣阿华州立大学研究基金会股份有限公司 | 提高葡糖淀粉酶pH最适点、底物专一性和热稳定性的蛋白质工程方法 |
| US20160002609A1 (en) * | 2013-01-28 | 2016-01-07 | Roche Diabetes Care, Inc. | Glucose Oxidase Variants, As Well As Compositions, Devices, Kits and Uses Thereof |
| CN108251389A (zh) * | 2017-08-18 | 2018-07-06 | 青岛蔚蓝生物集团有限公司 | 一种耐热性提高的葡萄糖氧化酶突变体 |
| US10233430B1 (en) * | 2017-10-13 | 2019-03-19 | Dongguan Apac Biotechnology Co.,Ltd. | Glucose oxidase having improved thermostability |
| CN109666657A (zh) * | 2017-10-13 | 2019-04-23 | 东莞泛亚太生物科技有限公司 | 提升耐热性的葡萄糖氧化酶 |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114736879A (zh) * | 2022-06-09 | 2022-07-12 | 中国农业科学院北京畜牧兽医研究所 | 热稳定性改善的葡萄糖氧化酶GoxM10突变体E361P及其衍生突变体和应用 |
| CN114736879B (zh) * | 2022-06-09 | 2022-09-27 | 中国农业科学院北京畜牧兽医研究所 | 热稳定性改善的葡萄糖氧化酶GoxM10突变体E361P及其衍生突变体和应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20220220454A1 (en) | 2022-07-14 |
| WO2020239064A1 (zh) | 2020-12-03 |
| EP3978602A4 (en) | 2023-07-05 |
| EP3978602A1 (en) | 2022-04-06 |
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