CN113797151B - 一种含间充质干细胞外泌体的修护凝胶及其制备方法 - Google Patents
一种含间充质干细胞外泌体的修护凝胶及其制备方法 Download PDFInfo
- Publication number
- CN113797151B CN113797151B CN202111174284.2A CN202111174284A CN113797151B CN 113797151 B CN113797151 B CN 113797151B CN 202111174284 A CN202111174284 A CN 202111174284A CN 113797151 B CN113797151 B CN 113797151B
- Authority
- CN
- China
- Prior art keywords
- parts
- exosome
- freeze
- stem cell
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000001808 exosome Anatomy 0.000 title claims abstract description 88
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title abstract description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 48
- 238000004108 freeze drying Methods 0.000 claims abstract description 22
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 14
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 14
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 14
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 14
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 11
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 11
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 11
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229940064064 purslane extract Drugs 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 8
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims abstract description 7
- 229920002498 Beta-glucan Polymers 0.000 claims abstract description 7
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 claims abstract description 7
- 239000004471 Glycine Substances 0.000 claims abstract description 7
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 claims abstract description 7
- 229940106189 ceramide Drugs 0.000 claims abstract description 7
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 claims abstract description 7
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229960003966 nicotinamide Drugs 0.000 claims abstract description 5
- 235000005152 nicotinamide Nutrition 0.000 claims abstract description 5
- 239000011570 nicotinamide Substances 0.000 claims abstract description 5
- 235000011187 glycerol Nutrition 0.000 claims description 16
- 239000008223 sterile water Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 14
- 238000005119 centrifugation Methods 0.000 claims description 11
- 239000003223 protective agent Substances 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 10
- 229920002125 Sokalan® Polymers 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 210000004027 cell Anatomy 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 claims description 5
- 210000000130 stem cell Anatomy 0.000 claims description 4
- 229920002307 Dextran Polymers 0.000 claims description 3
- 238000004113 cell culture Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000008176 lyophilized powder Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 210000003954 umbilical cord Anatomy 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims 1
- 230000003020 moisturizing effect Effects 0.000 abstract description 9
- 239000002537 cosmetic Substances 0.000 abstract description 4
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 3
- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 3
- 230000005847 immunogenicity Effects 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 3
- 206010002198 Anaphylactic reaction Diseases 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 208000003455 anaphylaxis Diseases 0.000 abstract description 2
- 230000004071 biological effect Effects 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 229940074410 trehalose Drugs 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 3
- 230000003716 rejuvenation Effects 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 239000000232 Lipid Bilayer Substances 0.000 description 1
- 208000002151 Pleural effusion Diseases 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8147—Homopolymers or copolymers of acids; Metal or ammonium salts thereof, e.g. crotonic acid, (meth)acrylic acid; Compositions of derivatives of such polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- Dermatology (AREA)
- Botany (AREA)
- Hematology (AREA)
- Rheumatology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Emergency Medicine (AREA)
- Dispersion Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明公开了一种含间充质干细胞外泌体的修护凝胶及其制备方法，属于化妆品技术领域，该修护凝胶由外泌体冻干粉和凝胶辅助组分组成。本发明中对外泌体进行先冻干处理再添加使用，避免了外泌体直接使用时其他组分对外泌体的破坏，从而增强了修护凝胶对皮肤的修复性能；同时其具有低免疫原性低，在皮肤表面使用时不会造成过敏反应；添加甘油、透明质酸钠、燕麦β‑葡聚糖、海藻糖、神经酰胺、烟酰胺作为本发明的保湿体系，加强凝胶的保湿性和成膜性，促进有效成分的经皮吸收，最大程度发挥本发明的皮肤修护功能；马齿苋提取物的添加使本发明具有消炎抗菌功效；甘油、甘氨酸、海藻糖复配的冻干保护液可以保护外泌体在冷冻干燥中的生物活性。
Description
技术领域
本发明涉及化妆品技术领域，具体涉及到一种含间充质干细胞外泌体的修护凝胶及其制备方法。
背景技术
外泌体(exosome)是哺乳动物细胞通过“内吞-外排”过程分泌到细胞外的一类囊泡状结构，直径约为30-150nm。外泌体不仅存在于细胞培养液中，也存在于血液、尿液、羊水、母乳、精液、唾液、胸腔积液等体液中，其组成包括脂质双分子层以及细胞来源相关的蛋白质、RNA、DNA等物质。
现有的凝胶类化妆品虽然种类非常繁多，但是对过敏感性肌肤引起的过敏不能够起到很好的修复，且功效单一。同时现有技术中并未见添加外泌体的修护凝胶的研究报道。因此，为了满足消费者对外泌体修护凝胶的需求，提供一种含间充质干细胞外泌体的修护凝胶及其制备方法也就显得十分的有意义。
发明内容
针对上述的不足，本发明的目的是提供一种含间充质干细胞外泌体的修护凝胶及其制备方法，可有效解决现有凝胶类化妆品对皮肤修复效果差和功效单一的问题；该修护凝胶兼具有修复皮肤、抗炎杀菌和保湿效果，可更好满足消费者的需求。
为达上述目的，本发明采取如下的技术方案：
本发明提供一种含间充质干细胞外泌体的修护凝胶，由外泌体冻干粉和凝胶辅助组分组成；
其中，外泌体冻干粉由质量比为1～2:1～2的外泌体提取液和冻干保护剂组成。
进一步地，外泌体冻干粉和凝胶辅助组分的质量比为2～10:1～5。
进一步地，外泌体提取液和冻干保护剂的质量比为1:1。
进一步地，外泌体提取液通过以下方法制得：
收集P3-P7代的脐带干细胞培养上清，转移上清于离心管中进行第一次离心去活细胞，第二次离心去除死细胞，取离心后的上清过滤，即可得到外泌体提取液。
进一步地，外泌体提取液的制备方法中第一次离心的参数为：温度为2～5℃；转速为300～400xg；离心时长为10～15min。
进一步地，外泌体提取液的制备方法中第二次离心的参数为：温度为2～5℃；转速为2000～3000xg；离心时长为10～15min。
进一步地，冻干保护剂由以下质量份的原料组成：甘油1～5份、甘氨酸1～5份、海藻糖1～3份和无菌水1～3份。
进一步地，冻干保护剂由以下质量份的原料组成：甘油3份、甘氨酸3份、海藻糖2份和无菌水2份。
进一步地，外泌体冻干粉通过以下方法制得：
步骤(1)：将外泌体提取液和冻干保护剂混合均匀得到外泌体冻干液；
步骤(2)：将步骤(1)所得的外泌体冻干液于-80℃～-70℃预冻48-56h，然后将预冻好的外泌体冻干液真空冷冻干燥，即可得到外泌体冻干粉。
进一步地，凝胶辅助组分包括以下质量份的原料：甘油2～6份、燕麦β-葡聚糖2～6份、透明质酸钠2～5份、马齿苋提取物1～4份、神经酰胺1～2份、烟酰胺1～2份、海藻糖1～2份、Carbopol Ultrez 20 0.5～2份和无菌水5～10份。
进一步地，凝胶辅助组分包括以下质量份的原料：甘油4份、燕麦β-葡聚糖4份、透明质酸钠3份、马齿苋提取物2份、神经酰胺1份、烟酰胺1份、海藻糖1份、Carbopol Ultrez20 1份和无菌水5～10份。
本发明中马齿苋提取物可直接购买市售产品。
本发明还提供上述含间充质干细胞外泌体的修护凝胶的制备方法，包括以下步骤：
步骤(1)：将外泌体冻干粉、透明质酸钠、甘油、马齿苋提取液、燕麦β-葡聚糖、烟酰胺和一半的无菌水加入容器中，搅拌均匀，得到溶液一；
步骤(2)：将剩下的一半无菌水加入容器中，加热至70℃～80℃，然后加入Carbopol Ultrez 20搅拌均匀，冷却，得到均匀透明的凝胶状溶液二；
步骤(3)：将步骤(2)所得的溶液二分批次加入步骤(1)所得的溶液一中，搅拌均匀，即可得到含间充质干细胞外泌体的修护凝胶。
综上所述，本发明具有以下优点：
1、本发明提供了一种含间充质干细胞外泌体的修护凝胶，该修护凝胶可以在皮肤表面形成一层保护薄膜，隔绝外界环境对皮肤的损害，同时添加外泌体可以对皮肤进行修护，维护皮肤状态。同时配合添加的透明质酸钠、甘油等作为本发明的保湿体系，添加马齿苋提取物做消炎抗菌体系。通过各组分间的协同作用，使得修护凝胶兼具有修复皮肤、抗炎杀菌和保湿效果，可更好满足消费者的需求。
2、本发明中选用的干细胞外泌体是一种高效的皮肤修复成分，同时来源于间充质干细胞，具有低免疫原性低，在皮肤表面使用时不会造成过敏反应；同时添加甘油、透明质酸钠、燕麦β-葡聚糖、海藻糖、神经酰胺、烟酰胺作为本发明的保湿体系，加强凝胶的保湿性和成膜性，促进有效成分的经皮吸收，最大程度发挥本发明的皮肤修护功能；马齿苋提取物的添加使本发明具有消炎抗菌功效；甘油、甘氨酸、海藻糖复配的冻干保护液可以保护外泌体在冷冻干燥中的生物活性。
3、本发明中对外泌体进行先冻干处理再添加使用，避免了外泌体直接使用时其他组分对外泌体的破坏，从而增强了修护凝胶对皮肤的修复性能。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅用以解释本发明，并不用于限定本发明，即所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。
因此，以下对提供的本发明的实施例的详细描述并非旨在限制要求保护的本发明的范围，而是仅仅表示本发明的选定实施例。基于本发明的实施例，本领域技术人员在没有做出创造性劳动的前提下所获得的所有其他实施例，都属于本发明保护的范围。
实施例1
本例提供一种含间充质干细胞外泌体的修护凝胶，由外泌体冻干粉和凝胶辅助组分组成；
其中，外泌体冻干粉和凝胶辅助组分的质量比为3:5；
凝胶辅助组分包括以下质量份的原料：甘油4份、燕麦β-葡聚糖4份、透明质酸钠3份、马齿苋提取物2份、神经酰胺1份、烟酰胺1份、海藻糖1份、Carbopol Ultrez 20 1份和无菌水8份；
外泌体冻干粉由质量比为1:1的外泌体提取液和冻干保护剂组成；
外泌体提取液通过以下方法制得：
收集生长状况良好的P3-P7代的脐带干细胞培养上清，转移上清于离心管中离心(4℃，300xg，10min)去活细胞，(4℃，2000xg，10min)去除死细胞；取离心后的上清用0.22μm过滤器过滤即可得到外泌体提取液；
冻干保护剂由以下质量份的原料组成：甘油3份、甘氨酸3份、海藻糖2份和无菌水2份；
外泌体冻干粉通过以下方法制得：
步骤(1)：将外泌体提取液和冻干保护剂混合均匀得到外泌体冻干液；
步骤(2)：将步骤(1)所得的外泌体冻干液于-80℃预冻48h，然后将预冻好的外泌体冻干液真空冷冻干燥，即可得到外泌体冻干粉。
本例中含间充质干细胞外泌体的修护凝胶的制备方法，包括以下步骤：
步骤(1)：将外泌体冻干粉、透明质酸钠、甘油、马齿苋提取液、燕麦β-葡聚糖、烟酰胺和一半的无菌水加入容器中，搅拌均匀，得到溶液一；
步骤(2)：将剩下的一半无菌水加入容器中，加热至70℃，然后加入CarbopolUltrez 20搅拌均匀，冷却，得到均匀透明的凝胶状溶液二；
步骤(3)：将步骤(2)所得的溶液二分批次加入步骤(1)所得的溶液一中，搅拌均匀，即可得到含间充质干细胞外泌体的修护凝胶。
实施例2
本例提供一种含间充质干细胞外泌体的修护凝胶，与实施例1的区别仅在于：凝胶辅助组分中Carbopol Ultrez 20为0.7份，其余参数及制备方法均相同。
实施例3
本例提供一种含间充质干细胞外泌体的修护凝胶，与实施例1的区别仅在于：冻干保护剂由以下质量份的原料组成：甘油5份、甘氨酸5份、海藻糖3份和无菌水3份；其余参数及制备方法均相同。
对比例1
本例提供一种含间充质干细胞外泌体的修护凝胶，与实施例1的区别仅在于：将外泌体提取液直接使用，即外泌体提取液未进行先冻干处理便与凝胶辅助组分配合使用；其余参数及制备方法均相同。
实验例
为验证本发明中含间充质干细胞外泌体的修护凝胶的皮肤修护功效，本例选择5组，每组5人分别编号为A、B、C、D、E，共25名年龄在20-40岁的每组受试者分别使用本发明实施例1-3和对比例1所得的修护凝胶30天。分别在使用开始前，使用10天、使用20天、使用30天分别使用VISIA-专业皮肤分析系统进行皮肤检测，检测指标包含：皱纹、毛孔、红色区、纹理。为方便统计，记录的是每次检测比初始值高/低的数值，同时收集受试者的使用感，测试结果如下表1-4所示。
表1实施例1所得含间充质干细胞外泌体的修护凝胶的测试结果
表2实施例2所得含间充质干细胞外泌体的修护凝胶的测试结果
表3实施例3所得含间充质干细胞外泌体的修护凝胶的测试结果
表4对比例1所得含间充质干细胞外泌体的修护凝胶的测试结果
通过表1-4的结果可以看出受试者皮肤的皱纹、毛孔、红色区、纹理在使用本发明产品后都有所好转，且随着使用时间的增长，情况越来越好。通过对受试者的面部观察可发现个受试者各自脸部情况都有所好转甚至消失，证明本发明的修护凝胶对皮肤有修护效果，修护效果随时间增加而增加，且明显优于对照组，说明本发明中对外泌体进行先冻干处理再添加使用，避免了外泌体直接使用时其他组分对外泌体的破坏，可有效增强修护凝胶对皮肤的修复性能。
以上内容仅仅是对本发明所作的举例和说明，所属本领域的技术人员不经创造性劳动即对所描述的具体实施例做的修改或补充或采用类似的方式替代仍属本专利的保护范围。
Claims (3)
1.一种含间充质干细胞外泌体的修护凝胶，其特征在于，所述修护凝胶由外泌体冻干粉和凝胶辅助组分组成；
其中，外泌体冻干粉由质量比为1~2:1~2的外泌体提取液和冻干保护剂制成；所述外泌体冻干粉通过以下方法制得：
步骤（1）：将外泌体提取液和冻干保护剂混合均匀得到外泌体冻干液；
步骤（2）：将步骤（1）所得的外泌体冻干液于-80℃~-70℃预冻48-56h，然后将预冻好的外泌体冻干液真空冷冻干燥，即得到外泌体冻干粉；
所述冻干保护剂由以下质量份的原料组成：甘油1~5份、甘氨酸1~5份、海藻糖1~3份和无菌水1~3份；
所述凝胶辅助组分包括以下质量份的原料：甘油2~6份、燕麦β-葡聚糖2~6份、透明质酸钠2~5份、马齿苋提取物1~4份、神经酰胺1~2份、烟酰胺1~2份、海藻糖1~2份、CarbopolUltrez 20 0.5~2份和无菌水5~10份；
所述含间充质干细胞外泌体的修护凝胶通过以下方法制得：
步骤（1）：将外泌体冻干粉、透明质酸钠、甘油、马齿苋提取液、燕麦β-葡聚糖、烟酰胺和一半的无菌水加入容器中，搅拌均匀，得到溶液一；
步骤（2）：将剩下的一半无菌水加入容器中，加热至70 ℃~80 ℃，然后加入CarbopolUltrez 20搅拌均匀，冷却，得到均匀透明的凝胶状溶液二；
步骤（3）：将步骤（2）所得的凝胶状溶液二分批次加入步骤（1）所得的溶液一中，搅拌均匀，即得到含间充质干细胞外泌体的修护凝胶。
2.如权利要求1所述的含间充质干细胞外泌体的修护凝胶，其特征在于，所述外泌体提取液通过以下方法制得：
收集P3-P7代的脐带干细胞培养上清，转移上清于离心管中进行第一次离心去活细胞，进行第二次离心去除死细胞，取离心后的上清过滤，即得到外泌体提取液。
3. 如权利要求1所述的含间充质干细胞外泌体的修护凝胶，其特征在于，所述凝胶辅助组分包括以下质量份的原料：甘油4份、燕麦β-葡聚糖4份、透明质酸钠3份、马齿苋提取物2份、神经酰胺1份、烟酰胺1份、海藻糖1份、Carbopol Ultrez 20 1份和无菌水5~10份。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111174284.2A CN113797151B (zh) | 2021-10-09 | 2021-10-09 | 一种含间充质干细胞外泌体的修护凝胶及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111174284.2A CN113797151B (zh) | 2021-10-09 | 2021-10-09 | 一种含间充质干细胞外泌体的修护凝胶及其制备方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113797151A CN113797151A (zh) | 2021-12-17 |
| CN113797151B true CN113797151B (zh) | 2023-10-31 |
Family
ID=78897418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111174284.2A Active CN113797151B (zh) | 2021-10-09 | 2021-10-09 | 一种含间充质干细胞外泌体的修护凝胶及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113797151B (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115721774A (zh) * | 2022-10-31 | 2023-03-03 | 博品(上海)生物医药科技有限公司 | 一种基于干细胞提取物的凝胶及其制备方法与应用 |
| CN117653606B (zh) * | 2023-11-14 | 2024-05-10 | 广东康百医药科技有限公司 | 一种间充质干细胞外泌体冻干保护剂及其应用 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190069301A (ko) * | 2017-12-11 | 2019-06-19 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 민감성 피부의 예방, 억제, 완화 또는 개선용 화장료 조성물 |
| CN110037979A (zh) * | 2019-04-25 | 2019-07-23 | 广州优理氏生物科技有限公司 | 含羊间充质干细胞外泌体冻干粉及其制备方法 |
| CN110339147A (zh) * | 2018-11-27 | 2019-10-18 | 浙江梵泊细胞工程有限公司 | 一种皮肤外用组合物及其用途 |
| CN110548001A (zh) * | 2019-09-06 | 2019-12-10 | 沈阳细胞治疗工程技术研发中心有限公司 | 一种含脐带间充质干细胞外泌体的修复抗衰护肤品 |
| CN111743836A (zh) * | 2020-07-08 | 2020-10-09 | 广州暨创医美生物科技有限公司 | 一种舒缓修护面膜液、及其制备方法和包含其的冻干面膜 |
| CN111888459A (zh) * | 2020-08-18 | 2020-11-06 | 深圳市俊元生物科技有限公司 | 一种含间充质干细胞分泌因子的冻干粉及其溶媒 |
| CN112516067A (zh) * | 2020-12-09 | 2021-03-19 | 广州美邦生物科技有限公司 | 一种含脐带间充质干细胞外泌体的组合物及其应用 |
-
2021
- 2021-10-09 CN CN202111174284.2A patent/CN113797151B/zh active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190069301A (ko) * | 2017-12-11 | 2019-06-19 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 민감성 피부의 예방, 억제, 완화 또는 개선용 화장료 조성물 |
| CN110339147A (zh) * | 2018-11-27 | 2019-10-18 | 浙江梵泊细胞工程有限公司 | 一种皮肤外用组合物及其用途 |
| CN110037979A (zh) * | 2019-04-25 | 2019-07-23 | 广州优理氏生物科技有限公司 | 含羊间充质干细胞外泌体冻干粉及其制备方法 |
| CN110548001A (zh) * | 2019-09-06 | 2019-12-10 | 沈阳细胞治疗工程技术研发中心有限公司 | 一种含脐带间充质干细胞外泌体的修复抗衰护肤品 |
| CN111743836A (zh) * | 2020-07-08 | 2020-10-09 | 广州暨创医美生物科技有限公司 | 一种舒缓修护面膜液、及其制备方法和包含其的冻干面膜 |
| CN111888459A (zh) * | 2020-08-18 | 2020-11-06 | 深圳市俊元生物科技有限公司 | 一种含间充质干细胞分泌因子的冻干粉及其溶媒 |
| CN112516067A (zh) * | 2020-12-09 | 2021-03-19 | 广州美邦生物科技有限公司 | 一种含脐带间充质干细胞外泌体的组合物及其应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113797151A (zh) | 2021-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113797151B (zh) | 一种含间充质干细胞外泌体的修护凝胶及其制备方法 | |
| CN110037979B (zh) | 含羊间充质干细胞外泌体冻干粉及其制备方法 | |
| CN110548001A (zh) | 一种含脐带间充质干细胞外泌体的修复抗衰护肤品 | |
| CN114306432B (zh) | 一种具有防脱生发功能的植物外泌体组合物及其制备方法 | |
| WO2020173164A1 (zh) | 间充质干细胞冻干粉剂及其制备方法 | |
| CN109453051B (zh) | 一种美白修复面膜及其制备方法 | |
| US20220287952A1 (en) | Compositions containing exosomes from animal placenta, methods for producing the same and uses thereof | |
| CN110693806A (zh) | 一种护肤组合物、护肤精华及其制备方法 | |
| CN111420023B (zh) | 含i型胶原和透明质酸的复合物及制备和用途 | |
| CN113750030A (zh) | 一种含外泌体的抗衰精华液及其制备方法 | |
| CN109453090A (zh) | 一种保湿美白、紧致提升的精华液组合物及其制备方法 | |
| CN116407475B (zh) | 一种洁面乳及其制备方法 | |
| CN110123953B (zh) | 一种促进术后皮肤修复的冷敷贴及其制备方法 | |
| CN115969750B (zh) | 一种舒缓修复组合物及其制备方法与应用 | |
| CN105147575A (zh) | 一种干细胞生发素及其制备方法 | |
| CN115957148B (zh) | 一种含复合植物抗菌肽的营养物及其微胶囊 | |
| KR101953676B1 (ko) | 악마의 발톱 초음파 추출물을 함유하는 피부 보습용 화장료 조성물 | |
| CN115814045A (zh) | 一种用于改善脱发的外泌体复合制剂及其制备方法与应用 | |
| CN115414308A (zh) | 一种祛痘组合物及制备方法和应用 | |
| CN115644170A (zh) | 一种经血保存液及制备方法和应用 | |
| CN115607490A (zh) | 用于抑制皮脂腺类炎症的护肤组合物及其制备方法 | |
| CN111714404B (zh) | 祛痘组合物及其制备方法和应用 | |
| CN108078906B (zh) | 一种烫染发用茶油发膜及其制作方法和应用 | |
| CN113244140A (zh) | 一种调节皮肤微生态平衡的组合物及其应用与制备方法 | |
| CN112089640A (zh) | 一种滋润美肤身体乳及其制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |