CN113795579A - 用于共转录加帽的rna聚合酶变体 - Google Patents

用于共转录加帽的rna聚合酶变体 Download PDF

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Publication number
CN113795579A
CN113795579A CN202080029500.1A CN202080029500A CN113795579A CN 113795579 A CN113795579 A CN 113795579A CN 202080029500 A CN202080029500 A CN 202080029500A CN 113795579 A CN113795579 A CN 113795579A
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amino acid
rna polymerase
acid substitution
rna
variant
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阿萨纳西奥斯·杜西斯
干乍那·拉维钱德兰
埃米·E·拉比多
玛格丽特·富兰克林
凯文·史密斯
米歇尔·林恩·哈尔
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ModernaTx Inc
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
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    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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CN202080029500.1A 2019-02-20 2020-02-19 用于共转录加帽的rna聚合酶变体 Pending CN113795579A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962808182P 2019-02-20 2019-02-20
US62/808,182 2019-02-20
US201962832314P 2019-04-11 2019-04-11
US62/832,314 2019-04-11
US201962885928P 2019-08-13 2019-08-13
US62/885,928 2019-08-13
PCT/US2020/018779 WO2020172239A1 (en) 2019-02-20 2020-02-19 Rna polymerase variants for co-transcriptional capping

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US (3) US11485960B2 (enExample)
EP (1) EP3927821A4 (enExample)
JP (2) JP2022521094A (enExample)
KR (1) KR20210142636A (enExample)
CN (1) CN113795579A (enExample)
AU (1) AU2020224103A1 (enExample)
CA (1) CA3130888A1 (enExample)
MA (1) MA55037A (enExample)
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WO (1) WO2020172239A1 (enExample)

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CN112921014A (zh) * 2019-12-05 2021-06-08 左炽健 T7RNA聚合酶突变体、mRNA、基因、表达载体及细胞
WO2024131998A3 (zh) * 2023-03-01 2024-08-08 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体、其制备方法及其在rna合成中的应用
CN118755694A (zh) * 2023-07-20 2024-10-11 上海金斯康生物科技有限公司 一种功能改进的t7 rna聚合酶变体
CN119464247A (zh) * 2025-01-13 2025-02-18 苏州近岸蛋白质科技股份有限公司 一种t7 rna聚合酶突变体及其应用
CN119490973A (zh) * 2023-08-15 2025-02-21 南京诺唯赞生物科技股份有限公司 提高体外转录中rna加帽率的rna聚合酶变体

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