CN1137897A - Animal blood hirudo hydrolytic method and hirudo hydrolytic blood - Google Patents
Animal blood hirudo hydrolytic method and hirudo hydrolytic blood Download PDFInfo
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- CN1137897A CN1137897A CN95105650A CN95105650A CN1137897A CN 1137897 A CN1137897 A CN 1137897A CN 95105650 A CN95105650 A CN 95105650A CN 95105650 A CN95105650 A CN 95105650A CN 1137897 A CN1137897 A CN 1137897A
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Abstract
The leech hydrolysis process for animal's blood features that leech is made to suck animal's blood fully and after several minites or hours, the blood is made to flow out of leech body physically or chemically and is collected, which contains low-molecular protein and peptide, hirudin, thrombase to digestblood, hyaluronidase and others for medical purpose. Used leech can be reused.
Description
The invention relates to method by the Hirudo hydrolysis of animal blood of edible animal blood and products thereof.
The animal blood and the product of making thereof are the very high nutraceutical of a kind of nutritive value.Because directly edible this group food can not fully be absorbed by human consumption, existing multinomial technology relates to the hydrolysis processing to blood powder, and as the technology of acid system and alkali process hydrolysis, enzymatic hydrolysis, wherein, technologies such as enzymolysis Sanguis sus domestica powder have obtained patent.But, the used enzyme of enzymatic hydrolysis of animal blood powder is generally animality enzyme (as pepsin, trypsin etc.) and bacterialprotease (as pronase, subtilisin etc.), the hydrolysis effect and the human body of these enzymes are close, and products obtained therefrom only is applicable to that general nutrition is required.
Data shows, blood-eating hirudo is as the toy of specially eating animal blood, and it is in sucking the biological process of animal blood, and the effect thing that is produced is very to help human health, human Blood stasis etc. is had special therapeutic efficiency, and its medicinal history is on the books in Ancient Chinese generation.As everyone knows, along with the raising of social life level, it is high that cardiac and cerebral vascular diseases is sent out rate in the human society, becomes first killer who influences human life and life quality, therefore Hirudo digests dissolved unique biological habit to blood, more and more draws attention.But up to now, with the method for the blood-eating hirudo hydrolysis of animal blood announcement that do not appear in the newspapers.
Since ancient times, Hirudo is used for medical science always.Ancient Chinese before more than 1,000 years is in the medical ancient books and records Shennong's Herbal, and is promptly on the books.At 19th-century, the medical application of Hirudo causes medical trematodiasis (Hlrudo mcdlclsalls), and almost it is exhausted, and impels Russia to impose the ration export tax.In recent years, secretions to Hirudo has carried out comparatively detailed scientific research, and find that it contains multiple biological products with broad-spectrum biological chemistry and pharmacological activity, as the hydrolytic enzyme of anticoagulant, metastasis agent, anesthetis, antibiotic, vasodilation and digestion blood.For example, with the isolated hirudin of the salivary gland of Hementaria officianalis (hlrdln) is the known the strongest thrombin inhibitor of the specific effect of tool, salivary gland with South America Hirudo (Hghlllanll) separates the hirudin that obtains, and is a kind of high-molecular weight fibrin (former) lyase.
For a long time, the utilization of Hirudo uses its corpse to carry tissue always, as the Hirudo medical material of Chinese medicine use, be with Hirudo after the process of preparing Chinese medicine, be used as medicine as medical material with its adult, it is reported, the consequence of handling like this is that the losses such as main anticoagulant composition in the Hirudo are very big.In addition, Hirudo salivary gland and the prepared tissue homogenate of Hirudo are carried out the method and the thinking of separation and Extraction, its outstanding shortcoming also is Hirudo must be put to death, this has just limited the access times of Hirudo, simultaneously, do not give full play to the biological efficiency of Hirudo digestion animal blood yet, wasted valuable resource.
The object of the present invention is to provide a kind of new animal blood and slime trematodiasis Hydrolyze method and Hirudohydrolysis blood thereof, make animal blood when molecular weight diminishes, obtain the haemolysis secretions of Hirudo.
Animal blood and slime trematodiasis Hydrolyze method of the present invention is as follows: make blood-eating hirudo fully draw animal blood, utilize physics or chemical method that blood is flowed out in the Hirudo body after several minutes to a few hours, collect effusive orange red blood body.
As above-mentioned animal blood and slime trematodiasis Hydrolyze method, it is characterized in that described physical method is a squeezing and pressing method.
Animal blood and slime trematodiasis Hydrolyze method as above-mentioned is characterized in that described physical method for Hirudo is placed in the airtight container, adds the bulk container internal gas pressure and makes the interior effusive pneumatic process of liquid of Hirudo abdomen.
Hirudohydrolysis blood, the animal blood of small molecular weight protein or peptide during it contains, the brinase (former) and the hyaluronidase that it is characterized in that also containing the excretory anticoagulant hirudin of Hirudo, digest blood.
Animal blood and slime trematodiasis Hydrolyze method of the present invention, biological nature with Hirudo happiness food animal blood, in the peak period of sucking blood in children's prime of life, it is fully received suckes, in its digestion blood makes the process of an amount of degraded of blood, secrete the brinase (former), hyaluronidase of hirudin, digestion blood and other and be difficult to the useful component measured at present, adopt then physical method (as extruding, air pressure, electricity thorn hit, broken abdomen etc.) or chemical method (as medicine emetic etc.), make Hirudo that the gastric thing is spued or overflows, collect liquid.This method is utilized the natural life power degraded blood of animal, technology is simple, obtain simultaneously also to have kept more precious excretory hirudin of Hirudo and brinase (former), hyaluronidase, and the useful composition that is difficult to measure at present makes this degraded blood increase medical value except that edibility comprehensively.The protein or the peptide of small-molecular weight also contained hirudin, protease and hyaluronidase during the Hirudohydrolysis blood that this kind method obtains contained, and its molecular weight overwhelming majority is thousands of to 100,000 dalton.
Hirudo can continue to use through restorative raising, and polypide can be made medical material by the Chinese medicine processing method to certain number of times, or utilizes method to be used by other of polypide, has improved the utilization rate of Hirudo greatly.
" blood-eating hirudo " among the present invention comprising: blood-eating hirudo (H.alpponlca whltmnn), beautiful doctor trematodiasis (H.Dwlchra Song) hirudo japonica (Haemadlpsa japoalca whitmnn), Tian Mu Shan Mountain doctor trematodiasis (H.tlanmnshasasong), South America Hirudo (Haemeaterla ghlllaall) etc. and other have the Hirudo of edible animal blood biological characteristics.
" blood " among the present invention comprises whole blood and component blood." animal " refers to comprise that primates, sheep, horse, cattle, pig, Canis familiaris L., deer, Bears, Trionyx sinensis Wiegmann, Testudinis of people etc. has the blood animal.
The liquid that is obtained among the present invention has favorable effects for degraded blood and Hirudo secretions mixture.Compare with the blood without Hirudo digestion, have following two characteristics at least: 1, contained macromolecular weight protein (as fibrin class, hemoglobin etc.) is degraded to molecular weight lower middle small molecular weight protein south or peptide, (100,000～number is done dalton).
2, the protease and the hyaluronidase isoreactivity composition that contain the excretory anticoagulant hirudin of Hirudo, digestion blood.
Compare with the enzymolysis Blood Law, contained Hirudo secretions is that enzymatic isolation method can not contain as anticoagulation thrombus dissolving material; Though the palliating degradation degree to big pheron is approaching or lower slightly, social benefit and economic benefit are fairly obvious.
Compare with hirudin method for making in the past, more economical than the parasite killing method of getting it filled, and increased the number of times that utilizes of Hirudo, improved the quantity of monomer Hirudo secretion hirudin, and, through the Hirudo that this law is utilized, still can be used as medicine by the Chinese medicine traditional method.To improve 2-10 doubly with the hirudin that this law was obtained than single killing method.
The liquid that obtains among the present invention, can directly use, the mother solution that also can be used for hydrolysis blood is used for external hydrolysis, can also be through further processing (as cultivating, activation, separation etc.) become single or the combination drug use, can also be used for substituting the prescription of some use Hirudo medical material in traditional Chinese medicine.
The liquid that obtains among the present invention has nourishing healthy (as the supplementary to anemia and weakling) effect, the effect of removing blood stasis, removing blood stasis are also arranged, stimulating the menstrual flow.Be mainly used in treatment and prevention ischemia anemi, various thrombosiss, particularly coronary artery and cerebrovascular disease, and other diseases that cause because of blood stasis.Indication relates to cardiovascular and cerebrovascular vessel, liver, kidney and dysemia, and woman, andrology's difficult miscellaneous diseases, wound pain and breathing, nervous system and cancer or the like also can be used for daily health carings such as beauty treatment, reduce wrinkle skin protection.
With regard to proteinic method for hydrolysis, the method for the biological characteristics aminosal of this class toy of Hirudo of the utilization happiness food animal blood among the present invention is to open one's minds.The present invention makes full use of Hirudo, promotes the well-being of mankind to make its good effect, and also be a contribution.The present invention has good social benefit and economic benefit.
Example:
The present invention can be illustrated with following infinite example.
Example one digests newborn calf's blood with blood-eating hirudo:
1,1-4 monthly age blood-eating hirudo is placed the vessel that contain newborn calf's blood, it is fully sucked blood;
2, the Hirudo after sucking blood makes digestion certain hour (several minutes～a few hours, depend on the needs) in 15-30 ℃,
3, (or additive method makes the Hirudo gastric thing that spues, and this thing is an orange red blood body, and blood smell is arranged with fashion of extrusion.Hirudo after the extruding more easily recovers to utilize.Through measuring, this liquid has following properties at least:
I, through the sds polyacrylamide gel electrophoresis analysis, during its protein molecule distributed, the macromolecule part was low than newborn calf's blood, the small-molecular weight part is then higher, this trend increases with the digestion time of Hirudo.Through sephadex chromatography, also obtain above-mentioned identical result;
II, through the vitro inhibition thrombin activity assay, have the active effect of Trombin inhibiting;
III, through the Proteolytic enzyme enzyme test, can digest hydrolysis hemoglobin, casein etc., have the hydrolyzed protein enzymatic activity.
4,, and make suitable for oral administration agent (tablet, electuary, capsule, oral liquid etc.) and other suitable dosage forms with the liquid freezing drying that obtains.Also can with it after suitable activation processing, behind the ultrafiltration membrance filter with molecular weight 10,000 or 20,000, make injection or other suitable dosage forms (as drop, liniment etc.).
Example two, be raw material with healthy human blood, 1-3 step in the reference example one, and make injection or lyophilized injection at last.
Example three, molten with the erythrocyte of livestock animals (cattle or pig or sheep) be raw material, 1-4 step in the reference example one is produced and is contained the abundant nutraceutical of enriching blood of haemachrome.The solution of red blood cells of livestock animals can derive from the gurry after biochemical pharmaceutical factory produces thrombin, has better economic and is worth and the environmental conservation effect.
Example four, be raw material with the fresh deer blood, reference example one is produced the nutriment that contains the effect that nourishes strong shape.
Example five, with the product that obtains among the present invention, be mixed with compound preparation in certain proportion with Chinese medicine Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Notoginseng, Flos Carthami, the Radix Astragali and other suitable medical materials.
Claims (4)
1, animal blood and slime trematodiasis Hydrolyze method is characterized in that making blood-eating hirudo fully to draw animal blood, with physics or chemical method blood is flowed out in the Hirudo body after several minutes to a few hours, collects effusive orange red blood body.
2, animal blood and slime trematodiasis Hydrolyze method as claimed in claim 1 is characterized in that described physical method is a squeezing and pressing method.
3, animal blood and slime trematodiasis Hydrolyze method as claimed in claim 1 is characterized in that described physical method for Hirudo is placed in the airtight container, adds the bulk container internal gas pressure and makes the interior effusive pneumatic process of liquid of Hirudo abdomen.
4, Hirudohydrolysis blood, the animal blood of small molecular weight protein or peptide is characterized in that also containing the excretory anticoagulant hirudin of Hirudo during it contained, the brinase (former) and the hyaluronidase of digestion blood.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95105650A CN1062741C (en) | 1995-06-09 | 1995-06-09 | Animal blood hirudo hydrolytic method and hirudo hydrolytic blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95105650A CN1062741C (en) | 1995-06-09 | 1995-06-09 | Animal blood hirudo hydrolytic method and hirudo hydrolytic blood |
Publications (2)
| Publication Number | Publication Date |
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| CN1137897A true CN1137897A (en) | 1996-12-18 |
| CN1062741C CN1062741C (en) | 2001-03-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN95105650A Expired - Fee Related CN1062741C (en) | 1995-06-09 | 1995-06-09 | Animal blood hirudo hydrolytic method and hirudo hydrolytic blood |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102085359A (en) * | 2011-01-14 | 2011-06-08 | 重庆时珍阁普生药业有限公司 | Medical composition containing hirudin and hementerin and application thereof |
| CN102114240A (en) * | 2011-01-14 | 2011-07-06 | 重庆时珍阁普生药业有限公司 | Application of medicament containing hirudin and cellosolve |
| CN102342263A (en) * | 2011-08-10 | 2012-02-08 | 重庆多普泰制药有限公司 | Method for improving content of hirudin in leech body |
| CN102659791A (en) * | 2012-04-12 | 2012-09-12 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting hemin and globin from animal blood |
| CN113577821A (en) * | 2021-07-07 | 2021-11-02 | 三峡大学 | Repeated extraction device and method for hirudin in living body |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077197A (en) * | 1992-04-11 | 1993-10-13 | 王林芳 | The extracting method of protoheme |
| CN1099620A (en) * | 1993-09-03 | 1995-03-08 | 吉林省商检应用技术研究所 | Biochemical preparing method for deer blood wine |
-
1995
- 1995-06-09 CN CN95105650A patent/CN1062741C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102085359A (en) * | 2011-01-14 | 2011-06-08 | 重庆时珍阁普生药业有限公司 | Medical composition containing hirudin and hementerin and application thereof |
| CN102114240A (en) * | 2011-01-14 | 2011-07-06 | 重庆时珍阁普生药业有限公司 | Application of medicament containing hirudin and cellosolve |
| CN102114240B (en) * | 2011-01-14 | 2013-07-17 | 重庆多普泰制药有限公司 | Application of medicament containing hirudin and cellosolve |
| CN102085359B (en) * | 2011-01-14 | 2013-07-17 | 重庆多普泰制药有限公司 | Medical composition containing hirudin and hementerin and application thereof |
| CN102342263A (en) * | 2011-08-10 | 2012-02-08 | 重庆多普泰制药有限公司 | Method for improving content of hirudin in leech body |
| CN102659791A (en) * | 2012-04-12 | 2012-09-12 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting hemin and globin from animal blood |
| CN102659791B (en) * | 2012-04-12 | 2015-01-07 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting hemin and globin from animal blood |
| CN113577821A (en) * | 2021-07-07 | 2021-11-02 | 三峡大学 | Repeated extraction device and method for hirudin in living body |
| CN113577821B (en) * | 2021-07-07 | 2022-08-30 | 三峡大学 | Repeated extraction device and method for hirudin in living body |
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| Publication number | Publication date |
|---|---|
| CN1062741C (en) | 2001-03-07 |
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