CN103462864A - Embryo water extract extracted from animal embryo internal organs, and extraction method and applications thereof - Google Patents
Embryo water extract extracted from animal embryo internal organs, and extraction method and applications thereof Download PDFInfo
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Abstract
The invention discloses an embryo water extract extracted from animal embryo internal organs, and an extraction method and applications thereof, and belongs to the field of extraction technology of embryo extract from animal embryos and applications of the extraction technology. The extraction method is capable of extracting the water soluble embryo extract without enzymatic hydrolysis by proteolytic enzyme, wherein the molecular weight of the water soluble embryo extract is less than 4000D. The extraction method is capable of increasing production efficiency and raw material utilization ratio, reducing production cost, meeting the requirements of cosmetic and food production, and ensuring easy absorption of the substances in the extract by human skin and mucosal tissues, and is beneficial to industrialized large-scaled production.
Description
Technical field
The present invention relates to from animal embryo, particularly, from the inner internal organs of the animal embryos such as sheep, pig and Canis familiaris L., comprise the Technology field of in embryo's the internal organs such as liver, heart, kidney, intestinal and blood, extracting embryo's element.
Background technology
Switzerland scientist Ka Er in 1912 professor is because finding first in the sheep embryonic cell that a kind of magical material that can make human body cell rejuvenate is arranged, i.e. sheep embryo extract and obtain Nobel Prize in medicine.The mankind find a kind of pure natural animal product that can slow down aging, i.e. sheep embryo extract, thus the dream that makes people pursue young and life lengthening becomes a reality.
According to science textual criticism, the number of times of biological cell division and have certain dependency between the life-span, such as, the chicken that the life-span is 30 years, cell division is 25 times; And the life-span is only the white mice of 3 years, only divide 12 times.And the basic reason that causes human senility is to form the most basic unit cell of human body life to have occurred aging; Along with the increase at age, cell division is more and more slower, and aged cells descends the organ function, even causes pathological changes, and the people is also thereupon old and feeble.The sheep embryo extract that Ka Er professor finds in the sheep embryo can make the animal body frequency dividing cell increase, and can make to have failed or aging cell reactivates or is eliminated, thereby aging function is restored.
1931, Switzerland Borrow. the mode of Ni Han professor first passage injection has also successfully been saved its life by the parathyroidin of lamb for an impaired patient that is dying of parathyroid gland, this have milestone formula meaning be found to be the mankind in the future sheep embryo extract in the application in biomedical and defying age field, lay a good foundation, the Pope, in order to mean the respect to him, awards Feng Qiwei the Vatican academician of academy of science.Nineteen fifty, Switzerland Dr.Alfred Pfister and professor Ni Han, through cooperation and effort for many years, find that the extract of sheep embryonic cell is the optimum carrier of competent cell material.
Nineteen fifty-two, Dr.Alfred Pfister has set up Switzerland's Lei Manna health medical treatment center, promotes sheep embryo extract living cells therapy.Nineteen fifty-five, the damage and the deterioration process that when under the graduate support of Switzerland Batelle, he has solved cell extraction, have very easily caused, and the technological deficiency of preserving, this achievement is named as Cellvital.1956, achievement in research Cellvital got back and once in 1931, obtains the Otto of Nobel Prize in medicine. Heinrich. Wa Erbao cell biological scholar's demonstration.
Over more than 70 year, whole world hundreds of thousands of them carried out the associated treatment of sheep embryo extract, comprising front Pope Borrow 12 generation, Queen of England and royalty member, Philippine president Marx, Saudi Arabia and Yemen king, Japanese Emperor Hirohito, mound gill prime minister, French gaullist president, Charlie Chaplin, and Norma Jean Remix, Dai Deli, Garbo, soccer star's Maradona etc.
Sheep embryo extract contains the multiple active component that can bring into play balance the body tissue organ function, and its Concentrations of Epidermal Growth Factor (EGF), hyaluronic acid stimulating factor (HASF) and basic fibroblast growth factor (bFGF) etc. have the effect of beautifying skin.Particularly the EGF in sheep embryo extract, just becoming fashion as a kind of emerging beauty and skin care factor.Research shows, people's corneocyte and fibrocyte etc. all have specificity EGF receptor to exist, and for the human skin cell has the ability that absorbs EGF, provides theoretical foundation.
In addition, also contain multiple small active peptides, nucleic acid, vitamin, several kinds of mineral elements and abundant aminoacid isoreactivity composition in sheep embryo extract, when promoting skin cell renewal, can also increase the content of its Endogenous Growth Factors, promote emiocytosis hyaluronic acid and glycoprotein, there is the adjustment skin texture, slow down aging, reduce wrinkle, suppress the effect of acne and the growth of control comedo.Therefore, sheep embryo extract will have more and more stronger vitality and good development prospect as a kind of cosmetics additive with unique biological effect.
Through century-old research, sheep placental extract is sure in the effect delayed aspect body aging; And its contained small-molecule active substance is also very significant in effect aspect the raising immunity of organisms; In addition, regulating the body hormonal readiness, improve anemia, strengthen metabolism and promote the aspects such as body vigor also to there is remarkable result.Sheep embryo extract be a kind of both can in use, active small molecular polypeptides matter that also can external, it is absorbed by body and the mode utilized is also diversified, its beauty treatment to the mankind and healthy cause will be played a greater and greater role.
The leaching process of sheep placental extract approximately can be divided into three phases at present: at first from lamb foetus organ cell, extract active component, remove impurity, thermo-responsive protein and the irritated factor; Secondly by isolation technics, the active substance that obtains having different efficacies, as embryonic thymus element, multiple somatomedin etc. dissimilar bioactive peptide, finally adopt freeze drying technology to carry out lyophilization, guarantees its activity.Extraction that it should be noted that sheep placental extract is a very complicated biotechnology processes, and nowadays most leaching process can only complete in laboratory, is difficult to carry out a large amount of suitability for industrialized production and long preservation.In China, there is laboratory to adopt the method for proteolytic enzyme enzymolysis to extract sheep embryo extract, although this method can increase substantially total protein content in hydrolyzed solution, but in the gross protein of finding to detect through current research, main increase is there is no the aminoacid of bio-regulating activity and the fragment of bioactive peptide, the embryo's cellulose content that really can bring into play regulating action is lower, the sheep embryo extract that the enzyme solution of explanation by proteolytic enzyme extracts is active and content is all lower, proteolytic enzyme has certain destruction to sheep embryo extract, present this extracting method is eliminated by new extracting method gradually.
Summary of the invention
The present invention's the first purpose is to propose a kind of embryo's element water extract extracted from the inner internal organs of animal embryo.
The molecular weight that water extract of the present invention extracts for the internal organs from animal embryo inside is less than the water solublity embryo element of 4000D.
Indication animal of the present invention is not sheep, can also be extended to the animals such as pig and Canis familiaris L., and the animal embryo internal organs can be as internal organs such as liver, heart, kidney, intestinal and blood, and the available embryo's commercial weight of the present invention is extensive, is easy to get, and is beneficial to industrialized mass.The molecular weight of water solublity embryo element of the present invention is less than 4000D can guarantee that in extracting solution, material is easily absorbed by human body skin and mucosal tissue.
The present invention's the second purpose is to invent a kind of method from the inner internal organs extract at low temperature of animal embryo embryo element that is beneficial to large-scale production, increases operation rate, reduces production costs.
The present invention includes following steps:
1) animal embryo that will freezing preservation under-15～-25 ℃ of temperature conditions is cleaned with distilled water, isolates internal organs in the embryo;
2) under condition of normal pressure, adopt distilled water that temperature is 0～5 ℃ to soak through internal organs in the embryo of mechanical activation comminution 20～30 minutes, in described distilled water and described embryo, the mixed weight of internal organs is than 1～4 ︰ 1;
3) will be repeatedly freezing and thaw 2～4 times by mixed liquor that in distilled water and embryo, internal organs are mixed to form, each freezing ambient temperature is-15～-30 ℃, each cooling time is 24～48 hours, thaws to 5 ℃ at every turn, in course of defrosting, adopts low-frequency ultrasonic waves to process 20～40 minutes;
4) with the refrigerated centrifuger of 4000～15000 rev/mins, above-mentioned mixed liquor is processed 10～15 minutes, removed solid impurity, obtain the water extraction solution A;
5) the Ph value of water extraction solution A is adjusted to 1.5～3.5, adopts the method for frozen centrifugation to remove precipitate, get supernatant and adjust again Ph value to 3.5～6.5, then remove precipitate through the method for frozen centrifugation, obtain the water extraction solution B;
6) under the ambient temperature of 1～5 ℃, the water extraction solution B is carried out to ultrafiltration, isolating molecular weight is the following water solublity embryo element of 4000D.
Raw material of the present invention adopts the animal embryo of freezing preservation, farthest keeps the activity of embryo's element in the embryo; The purpose that adopts distilled water to soak is the purity improved while extracting embryo's element, reduces the pollution that in water, impurity brings, and the distilled water temperature keeps 0～5 ℃, is conducive to protect the activity of embryo's element in extracting solution; Through the freezing and processing method of thawing repeatedly, make mixed liquor be the meat paste shape, purpose is all in order to improve extraction efficiency, increases the content of embryo's element in extracting solution, and farthest guarantees the activity of embryo's element in leaching process, is beneficial to follow-up separation; The Ph value that adopts first water transfer to put forward solution A is adjusted to 1.5～3.5, adjusts twice frozen centrifugation method of Ph value to 3.5～6.5 again, can efficiently remove the large protein in water extraction liquid A, is beneficial to follow-up ultrafiltration and extracts; Under the ambient temperature of 1～5 ℃, the water extraction solution B is carried out to ultrafiltration, purpose is the impact that the activity of guaranteeing to extract embryo's element is not subject to temperature, is beneficial to and extracts efficiently active effective ingredient.The natural conditions that final products pH value and embryo element exists are close, can fully guarantee the natural activity of embryo's element of extracting.
Invention just can extract the high activity embryo element of natural structure without the enzymolysis of proteolytic enzyme, improve embryo's cellulose content, be beneficial to industrial-scale production, enhance productivity, improve the utilization rate of raw material, to reduce production costs, can meet cosmetics and food production.
The animal embryo age in days of step 1) of the present invention is 30～145 days, and purpose is to make the content of embryo's element in extracted embryo more.
Separately, in described step 5), the water extraction solution A of 0～5 ℃ being carried out to the adjustment of Ph value, is also the activity that more advantageously keeps the effective ingredient in the water extraction solution A.
The 3rd purpose of the present invention is to propose the purposes that above molecular weight is less than the water solublity embryo element of 4000D.
One of purposes: the purposes of embryo's element water extract in Cosmetic Manufacture of extracting from the inner internal organs of animal embryo, characteristics are: when producing cosmetics, the water solublity embryo element that molecular weight is less than to 4000D accounts for 5～50% ratio in the quality that feeds intake and replaces original water in cosmetics.
Two of purposes: the purposes of embryo's element water extract in Cosmetic Manufacture of extracting from the inner internal organs of animal embryo, characteristics are: when producing cosmetics, the water solublity embryo element that molecular weight is less than to 4000D, after lyophilization, packs and makes cosmetics.
Through on probation, above two class cosmetics are remarkable to cosmetic result, and have no side effect, and expense is cheap, simple.
Three of purposes: the embryo's element water extract extracted from the inner internal organs of animal embryo is in the purposes of food production, and characteristics are: molecular weight is less than to the water solublity embryo element of 4000D for the preparation of health care oral liquid and capsule.
The present invention is easy to use, good absorbing effect, cheap as the advantage of health care oral liquid and capsule, can be used as general health product and immunostimulant and uses.
The specific embodiment
One, molecular weight is less than the extracting method of the water solublity embryo element of 4000D:
1, getting 30～145 age in days fresh animal embryos is placed under-15～-25 ℃ of cryogenic conditions and stores.
2, the animal embryo of freezing preservation is cleaned with distilled water, and separated the internal organs such as fetal liver, heart, kidney, intestinal and blood, then the employing of the internal organs after separating mechanical activation comminution, stand-by.
3,, under condition of normal pressure, in the embryo of the distilled water immersion process mechanical activation comminution that the employing temperature is 0～5 ℃, internal organs are 20～30 minutes.In distilled water and embryo, the mixed weight of internal organs is than 1～4 ︰ 1.
The mixed liquor iterative cycles that 4, will be mixed to form by internal organs in distilled water and embryo is freezing and thaw and process 4 times, each freezing ambient temperature is-15～-30 ℃, each cooling time is 24～48 hours, thaws to 5 ℃ at every turn, in course of defrosting, adopts low-frequency ultrasonic waves to process 20～40 minutes.
5, with the refrigerated centrifuger of 4000～15000 rev/mins, above-mentioned freezing through iterative cycles and mixed liquor that thaw is processed 10～15 minutes, removed solid impurity, obtain the water extraction solution A.
6, the water extraction solution A of 0～5 ℃ is adjusted to Ph value to 1.5～3.5 with 1～2N hydrochloric acid, adopt the method for frozen centrifugation to remove precipitate, get supernatant.The supernatant of 0～5 ℃ is adjusted to Ph value to 3.5～6.5 with 1～2N sodium hydroxide, then remove precipitate through the method for frozen centrifugation, obtain the water extraction solution B.
7, under the ambient temperature of 1～5 ℃, to the water extraction solution B, adopt the ultrafilter of 4000D to carry out ultrafiltration, isolating molecular weight is the following water solublity embryo element of 4000D.
Two, molecular weight is less than the evaluation of the water solublity embryo element of 4000D:
Adopt embryo's cellulose content in the supernatant after the nanodrop2000 ultraviolet spectrometry is measured ultrafiltration, embryo's cellulose content of the present invention can be reached for 0.4%～4% after measured, be 2～10 times of traditional embryo element extracting method content, can increase substantially extraction efficiency and the activity of embryo's element.
Three, prepare cosmetics:
1, cosmetics one:
Factory formula as astringent:
Take respectively water solublity embryo element 5kg, the 95kg that purifies waste water that molecular weight is less than 4000D by above raw material mix homogeneously, carry out bottledly, make astringent.
2, two of cosmetics:
Freeze-dried preparation as embryo's element water extract:
The water solublity embryo element that molecular weight is less than to 4000D, after lyophilization, packs and makes freeze-dried preparation.
Above astringent is directly used in respectively to the face after clean, by freeze-dried preparation directly or adopt introducer for the face after clean, absorbs faster than mill run.
Be applied to the skin aging crowd, it is remarkable that skin recovers elastic effect, and have no side effect.
Four, prepare health product:
1, prepare embryo's element oral formulations A:
By molecular weight, be that water solublity embryo below 4000D element is mixed well with Mel or other food additive, after ultrafiltration and pasteurization sterilizing, the bottled oral liquid of fill.
2, prepare embryo's element oral formulations B:
After being the water solublity embryo element lyophilization below 4000D, molecular weight obtains lyophilized powder as the raw material of producing various capsules.
3, prepare embryo's cellulose capsule:
By molecular weight, be to obtain 5 kilograms of lyophilized powders after the lyophilization of water solublity embryo below 4000D element, 95 kilograms of the lyophilized powders after the Flos Camelliae Japonicae powder lyophilization, the two is granulated after mixing, and the hard capsule that every net content of fill is 250 milligrams gets final product.
4, application:
Take object: the physical weakness person.
Taking dose: when oral liquid is used, according to 4～10 milliliters for each person every day, connect and take 20～30 days.Oral capsule, as capsule content is 10～50 milligrams/of lyophilized powders, everyone takes 20～30 days continuously according to 1～4/day.
Often easy catching a cold person and hyperlipemia, took product of the present invention about 20 days, and body immunity improves not easy catching a cold, and it is normal that the hyperlipidemia index can be recovered.
Claims (7)
1. the embryo's element water extract extracted from the inner internal organs of animal embryo, is characterized in that molecular weight that described water extract extracts for the internal organs from animal embryo inside is less than the water solublity embryo element of 4000D.
2. the preparation method of the embryo's element water extract extracted from the inner internal organs of animal embryo as claimed in claim 1 is characterized in that comprising the following steps:
1) animal embryo that will freezing preservation under-15～-25 ℃ of temperature conditions is cleaned with distilled water, isolates internal organs in the embryo;
2) under condition of normal pressure, adopt distilled water that temperature is 0～5 ℃ to soak through internal organs in the embryo of mechanical activation comminution 20～30 minutes, in described distilled water and described embryo, the mixed weight of internal organs is than 1～4 ︰ 1;
3) will be repeatedly freezing and thaw 2～4 times by mixed liquor that in distilled water and embryo, internal organs are mixed to form, each freezing ambient temperature is-15～-30 ℃, each cooling time is 24～48 hours, thaws to 5 ℃ at every turn, in course of defrosting, adopts low-frequency ultrasonic waves to process 20～40 minutes;
4) with the refrigerated centrifuger of 4000～15000 rev/mins, above-mentioned mixed liquor is processed 10～15 minutes, removed solid impurity, obtain the water extraction solution A;
5) the Ph value of water extraction solution A is adjusted to 1.5～3.5, adopts the method for frozen centrifugation to remove precipitate, get supernatant and adjust again Ph value to 3.5～6.5, then remove precipitate through the method for frozen centrifugation, obtain the water extraction solution B;
6) under the ambient temperature of 1～5 ℃, the water extraction solution B is carried out to ultrafiltration, isolating molecular weight is the following water solublity embryo element of 4000D.
3. method according to claim 2, the animal embryo age in days that it is characterized in that described step 1) is 30～145 days.
4. method according to claim 2, is characterized in that in described step 5) the water extraction solution A of 0～5 ℃ is carried out the adjustment of Ph value.
5. the purposes of embryo's element water extract in Cosmetic Manufacture of extracting from the inner internal organs of animal embryo as claimed in claim 1, it is characterized in that, when producing cosmetics, the water solublity embryo element that molecular weight is less than to 4000D accounts for 5～50% ratio in the quality that feeds intake and replaces original water in cosmetics.
6. the purposes of embryo's element water extract in Cosmetic Manufacture of extracting from the inner internal organs of animal embryo as claimed in claim 1, it is characterized in that, when producing cosmetics, the water solublity embryo element that molecular weight is less than to 4000D, after lyophilization, packs and makes cosmetics.
7. the embryo's element water extract extracted from the inner internal organs of animal embryo as claimed in claim 1 in the purposes of food production, is characterized in that, molecular weight is less than to the water solublity embryo element of 4000D for the preparation of health care oral liquid and capsule.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105411874A (en) * | 2015-12-30 | 2016-03-23 | 耿威 | Chick embryo bioactive peptide, preparation method and applications |
| CN105646647A (en) * | 2015-12-30 | 2016-06-08 | 耿威 | Chick embryo polypeptide as well as preparation method and application of chick embryo polypeptide |
| CN106562995A (en) * | 2016-10-26 | 2017-04-19 | 沈会强 | Preparation method for preparing swan embryos and application of swan embryos |
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| CN106821939A (en) * | 2017-03-19 | 2017-06-13 | 湖南海济药业有限公司 | The extracting method of sheep embryo extract and the preparation containing its extract |
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| CN109908181A (en) * | 2019-04-23 | 2019-06-21 | 独角兽生命科学股份公司 | The preparation method of anti-aging Porcine HGF |
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| CN1307871A (en) * | 2000-12-26 | 2001-08-15 | 邓开仲 | Goat fetus essence injecta and its preparation process |
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Patent Citations (1)
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| CN1307871A (en) * | 2000-12-26 | 2001-08-15 | 邓开仲 | Goat fetus essence injecta and its preparation process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105411874A (en) * | 2015-12-30 | 2016-03-23 | 耿威 | Chick embryo bioactive peptide, preparation method and applications |
| CN105646647A (en) * | 2015-12-30 | 2016-06-08 | 耿威 | Chick embryo polypeptide as well as preparation method and application of chick embryo polypeptide |
| CN106562995A (en) * | 2016-10-26 | 2017-04-19 | 沈会强 | Preparation method for preparing swan embryos and application of swan embryos |
| CN106562993A (en) * | 2016-10-26 | 2017-04-19 | 沈会强 | Preparation method and application of sika deer embryo factors |
| CN106581467A (en) * | 2016-10-26 | 2017-04-26 | 沈会强 | Corn embryonic factor manufacturing method and application |
| CN106581466A (en) * | 2016-10-26 | 2017-04-26 | 沈会强 | Manufacturing method and applications of rye embryo extract |
| CN108323750A (en) * | 2017-01-20 | 2018-07-27 | 内蒙古草原鑫河食品有限公司 | A kind of active constituent and its extracting method and application with liver protection |
| CN106821939A (en) * | 2017-03-19 | 2017-06-13 | 湖南海济药业有限公司 | The extracting method of sheep embryo extract and the preparation containing its extract |
| CN109908181A (en) * | 2019-04-23 | 2019-06-21 | 独角兽生命科学股份公司 | The preparation method of anti-aging Porcine HGF |
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