CN106581467A - Corn embryonic factor manufacturing method and application - Google Patents

Abstract

The invention discloses a corn embryonic factor manufacturing method, which comprises the following processes that corn embryonic stem cells are subjected to cell wall breaking treatment; impurity removal is performed; and after centrifugation, a corn pluripotent embryonic factor is obtained. The invention also discloses the corn embryonic factor obtained by the manufacturing method and application of the corn embryonic factor. The corn pluripotent embryonic factor is obtained through the separation from corn pluripotent embryonic stem cells; the effects of human embryonic factors are achieved, i.e., various cells in the human body maintain vitality; the service lives of work cells are prolonged; injured cells are repaired; the functions of the cells are recovered; the in vivo quiescent cells are activated; the effective work cells are supplemented; the attenuation of self stem cells is protected; the generation of stem cells is promoted; the self quiescent stem cells are activated to differentiate new cells for replacing dead cells; and the effect of recovering the visceral organs is achieved.

Description

Corn eggembryosin preparation method and application

Technical field

The present invention relates to drug medication, food and cosmetics technical field, especially a kind of corn eggembryosin, specifically addresses The preparation method of the corn eggembryosin and application.

Background technology

Although human diseases has thousands of kinds, but real disease only has one kind, that is, cytopathy, any one organ Cell metabolism it is out of joint, cell death reaches to a certain degree, then this organ just can it is sick, cause cell failure Main reasons is that two aspects：One is cytotrophy, oxygen supply is not enough, and two is that metabolic waste can not in time be discharged and cause cell The reason for caused by poisoning, and the present understanding to disease is the performance of disease, rather than real, mostly controls to the ill in treatment Treat (such as pain, the process of heating) or to treatment-resistant (excision to tumour, Radiotherapy chemotherapy etc.), most diseases are all right Disease is controlled.This research is to go out new functioning cell using stem cell division in this group of biomolecule human activin of corn eggembryosin to substitute Damage and dead cell, and recover and normal function, reach the purpose for fundamentally treating disease.

1912, Switzerland scientist karr professor had found to have in sheep embryonic cell first and a kind of can make what cell rejuvenated Magical material -- sheep embryo extract；1931, the mode of Ni Han professor's first passage injections was by the parathyroid cells application of lamb Yu Yiwei impaired patients In danger of parathyroid gland in operation have saved its life；Nineteen thirty-seven, professor Ni Han for the first time will be living thin Born of the same parents' therapy is applied to brain nervous cell, after, living cells therapy is extended to liver, pancreas, kidney, the heart by professor Ni Han Dirty, duodenum, thymus gland and spleen.Because sheep embryo extract is from the embryonic cell of sheep, there are all many-sides not with human cell Together, there are many contraindications, such as over anaphylaxis crowd during clinical practice, hyperthyroidism, serious hyperpietic are pregnant Phase women's use, tumor patient etc. can not be applied.

Because fresh and alive cell can not be preserved for a long time, the scientist of 1949 Nian Nihan professors and Nestle company assists jointly Make, lyophilized living cells is developed first carries out cell activation treatment；Nineteen fifty-five Switzerland Dr.Alfred doctors of medicine Pfister portion Divide the defect for (freezing or freezing) damage and deteriorating course and the Techniques of preserving for causing when solving cell extraction.

Martin Evans in 1970 are isolated from mouse blastular go out mouse embryo stem cell with vitro culture first (embryonic stem cell, ESCs, abbreviation ES, EK or ESC cell.)；American scientist vitro culture of human embryo in 1998 Stem cell success, hereafter using the different disease of different stem-cell therapies, its short effect can agree Chinese scholars It is fixed, but presently, there are problems with：Either sheep placenta or embryonic stem cell, due to being that xenogenesis (sheep) is all existed with allosome Polluted bacteria causes the severe complications such as general infection when immunological rejection, allergy, in vitro culture.

1980, because fresh and alive sheep embryo extract lacks the strict antibacterial measure of sterilization, it is impossible to long-term to preserve, there is infection Dangerous very big, Switzerland's medical circle is devoted to always development purification, and sterilization is sterilized, Techniques of preserving, but still can not reach long-time The purpose of preservation.

Nineteen eighty-three, Edward doctor Bei Chi inherits the achievement of Buddhist nun's writing brush sheep embryo extract active therapy, develops personalization Treatment technology, purifies " the full organ essence " for small molecule, more easily absorbs；Help human body activation and newly to bear structure complete It is whole, the active neoblast of function；Replace due to aging sick cell caused by various cause noxa element institutes, strengthen each life system Function, recovers human body energy and muscle power, reaches more significantly anti-aging curative effect.

American scientist vitro culture of human embryo's stem cell success in 1998.

Hereafter, using the different disease of different stem-cell therapies, its short effect can be affirmed Chinese scholars, But presently, there are problems with：Either sheep placenta or human embryo stem cell, due to being xenogenesis (sheep) and variant cell, after use Polluted bacteria causes the severe complications such as general infection when patient has immunological rejection, allergy, in vitro culture.People's embryo Tire stem cell also has ethics and legal issue.These problems are avoided, over nearly more than 160 years, scientist is dreamed of from plant Plant stem cell is isolated in separate living tissue or forming layer, although doing many painstaking efforts, is but never obtained into Work(, or even it is believed that this is impossible mythology.

2005, Korea cloud fire science and technology institute was successfully separated first in the world and has cultivated plant stem cell, and by its It is named as many Bels (Ddobyul).Research has shown that plant stem cell has powerful anti-oxidant and activity of fighting against senium, can be significantly Improve body immunity and anti-cancer ability.

Plant stem cell (Plant stem cell) includes all formulas with regard to development of plants and growth, possesses forever The cell (immortal cell) of permanent vitality, is the root (origin) of plant life power.Plant stem cell is present in and is claimed For in merismatic special tectonic, with very surprising power of regeneration.These allow plant continuous between centuries Growth, and generate brand-new organ.The research in stem cell clinical testing application has shown that stem cell animal can break up becomes group Knit cell, stem cell animal repair damaged tissues it was determined that but plant stem cell is huge with stem cell animal difference, plant is dry Cell can only break up becomes other kinds of plant cell, absolutely not to differentiate human cell.So, directly using plant Stem cell is used to repair the damage of human tissue cells can not reality.

The content of the invention

The present invention is directed to the deficiencies in the prior art, because plant stem cell cannot be directly used to repair the damage of human cell, So the effective ingredient of plant stem cell whether can the division of activation of human autologous stem cells realizing repairing and supplement human tissue Cell, reaches the purpose of rehabilitationThe present inventor proposes a kind of corn eggembryosin preparation method and application, and proves plant Eggembryosin can repair the stem cell division of resting state in the cell of body injury and activation body, produce new cell replacement dead The cell died, and its effect is played, the effect of fundamentally disease preventing and treating is reached, and it is easy to operate, effect is extensive, so as to solve This technical barrier.

Present inventor is from corn as research object.Corn (Classification system：Zea mays L, English：Corn) it is standing grain Undergraduate course Zea annual herb plant.Stalk is upright, generally not branch, and base portion respectively saves tool gas life prop root.The cultivation of China's corn Training history is about more than 470 year.Corn is the main food of the mankind, it can also be used to make wine, refine sugar etc. edible.Medical value：It is beautiful Rice must have diuresis, step-down, cholagogic, it is hypoglycemic, prevent constipation, prevent artery sclerosis, skin makeup skin care, hypoglycemic, the work(of prevention of tumor Effect.Rice sprout research is had shown that with Development of Modern Science, a large amount of proteases are contained in rice sprout, and vitamin A, B family vitamins, leaf is yellow The multiclass nutriment such as element, starch and protein, extracts its composition as medicinal.

The present invention provides technical scheme below：

A kind of preparation method of corn eggembryosin, comprises the following steps：

(1) elite corn kind；

(2) nursery, is dried prior to seeding for 8 days, dry 1～2 day after with 1% limewash immersion 2～3 days, then It is soaked in water 4～5 days, vernalization 8 days；

(3) collect corn embryo and obtain corn embryonic stem cell；

(4) breaking-wall cell process is carried out to corn embryonic stem cell, carries out impurity elimination process successively afterwards, centrifugal treating is obtained To corn pluripotent embryonic element；

The corn embryonic stem cell is multipotency corn embryonic stem cell.

Preferably, in the step (3), shred after the corn embryo for collecting is cleaned, add 3～5 times of distilled water Beating is crushed afterwards, is obtained the slurry of the corn embryo containing corn embryonic stem cell, then is obtained corn embryo after 200 mesh are filtered and does Cell.

Preferably, in the step (4), the breaking-wall cell processes using low-temperature and high-speed bead mill method that corn embryo is dry thin Born of the same parents' broken wall, the centrifugal treating is the 3000r/min under 5 DEG C -50 DEG C of temperature conditionss, and 10-15 minutes are centrifuged.

Preferably, also include that the corn eggembryosin to obtaining carries out sterilization treatment, 10 hours are continued at a temperature of 60 DEG C.

The present invention also provides a kind of corn eggembryosin, is obtained using above-mentioned preparation method.

Preferably, the corn eggembryosin include 42% Co-Q10,6% amino acid, 5% EGF, 41% Arbutin, nucleic acid, pantothenic acid, bacopa monnieri, lutein, epidermis reparative factor, superoxide dismutase, the maize The purity of tire element is more than 98%.

The present invention also provides a kind of purposes of corn eggembryosin, refers to for preparing treatment cardiovascular and cerebrovascular, diabetes, stomach 12 Enterelcosis, hepatitis, the medicine of the chronic disease of renal failure and anti-aging and beautification product.

Preferably, the formulation of the medicine or product includes oral administration pills, oral liquid, external application emulsion, is administered to medicament.

Preferably, the corn eggembryosin accounts for the 1%- of unit dose drug or product in medicine or product is prepared 12%.

Compared with prior art, the present invention has advantages below：The isolated corn multipotency from corn embryonic stem cell Eggembryosin mixture (mostly small-molecular peptides) is by oral administration or local topical can directly absorb.CEF is entered after human body and can be activated itself Dormancy stem cell differentiates the dead cell of new cell substitute, reaches the effect for restoring internal organs, and it had both exceeded current stem cell The therapeutic action of transplanting, and without the complication being likely to occur after stem cell transplantation, such as immunological rejection, medication way levy conveniently, Safety, can also avoid the problem of Human embryo element ethics and law, be fundamentally to prevent and treat all kinds of, various diseases, especially chronic, Difficult disease, anti-aging, prolongation life-span, beauty etc. are optimal natural Health preservation therapies.

Specific embodiment

Describe the present invention with reference to embodiment, the description of this part be only it is exemplary and explanatory, no Reply protection scope of the present invention has any restriction effect.

The present invention is the jade by extracting from corn embryonic stem cell (corn embryonic stem cell, CESC) Rice eggembryosin (corn embryonic factor, CEF), is currently viewed as all kinds of incurable disease diseases, especially for preventing and treating It is all kinds of chronic, difficult diseases and anti-aging and beauty.

The action principle of CEF：Alternative Human embryos element (HEF) of CEF, can keep cell viability, extend the longevity of working cardial cell Life；Damaging cells are repaired, recovers the function of cell；Extend the life-span of internal stem cell；Internal rest cell is activated, supplements effective Working cardial cell；Itself dormancy stem cell is activated, breaks up different tissues cell, to substitute the thin of aging, damage and death is updated Born of the same parents, restore histoorgan, reach source radical curing of disease, reverse the effect in youth.

Corn embryonic stem cell, in being present in corn embryo, from the corn nursery maize of the 8th day (1～3 leaf of corn During rice seedling), including cotyledon, plumule, plumular axis, radicle and endosperm, Eradicates is except hoja, all kinds of risk factors and impurity, maize There is substantial amounts of corn embryonic stem cell in tire, be not required to be kept completely separate out corn embryonic stem cell, therefore one piece is collected, and obtain beautiful Rice embryonic stem cell and corn other cells.

Corn embryo (rice seedling) is cleaned, rinsed repeatedly, shredded with sterile distilled water, by 1：5 ratio adds aseptic steaming Distilled water crushes beating, obtains corn embryo slurry, embryonic stem cell containing corn, corn other histocytes, string, tissue fluid Deng composition, elimination string is crossed, retain the compositions such as corn embryonic stem cell, corn other histocytes, tissue fluid.

Corn embryonic stem cell adopts low-temperature and high-speed bead mill method broken wall treatment, including cell membrane and nucleus mould, while can The worm's ovum suitable with cell dimensions, bacterium also can be killed, most after Jing centrifugation after obtain corn eggembryosin.

Corn eggembryosin of the present invention is pluripotent embryonic element.

Due to taking in 8 days in corn embryo, its overwhelming majority is pluripotent embryonic stem cells to CEF of the present invention, so obtaining Eggembryosin be pluripotent embryonic element, its effect can keep cell viability, extend the life-span of working cardial cell；Repair damaging cells, Recover the function of cell；Activate internal rest cell, the effective working cardial cell of supplement；The decay and promotion of protection autologous stem cells Stem cell generates；Activate itself dormancy stem cell and differentiate various types of cells, dead cell of substituting reaches the work for restoring internal organs With for treating various diseases, especially chronic or difficult disease is very effective with anti-aging curative effect, and the product can be protected for a long time Deposit, it is non-toxic, without rejection, medication orally with external application, convenience, safety, can also avoid asking for the plain ethics of Human embryo and law Topic.

Embodiment 1

The present embodiment provides a kind of preparation method of corn eggembryosin

1. corn embryonic tissue is obtained

1.1 elite corn kinds：

Select the requirement of maize seed：It is preferred from high yield, health is disease-free, maize seed then.The present embodiment is from Heilungkiang agriculture Institute of section Seed-breeding base obtains high-quality, high yield, disease-resistant hybrid maize combination (kind).Different, the seed rice quantity according to output demand It is different, can be with 10,100,500 kilograms as a radix, the present invention is according to test with experimental measuring with 500 kilograms of corns as a base Number.

1.2 correct nursery：

Prior to seeding 8d is basked seeds, and to be shone and soak 2～3d with 1% limewash after 1～2d, is beneficial to disinfection, is promoted Enter seed sprouting.Then cleaned with clear water and use again 4～6d of cold water soak, make seed fully absorb water, note changing water and stirring, finally Vernalization 8d is filtered, it is seen that when germinal layer, plumule, plumular axis, radicle and endosperm, embryo seedling about 1.5cm or so, as corn embryo..

1.3 cleaning embryo seedlings：

When collecting corn embryo, by clean operation, subsequently soak embryo seedling 30min with sterile pure water, Eradicates except it is all kinds of it is dangerous because Son, such as impurity, rinse and are inserted in sterile bag after corn embryonic tissue repeatedly..

2. stem cell is obtained from corn embryo

Embryo is extracted in 2.1 corn embryos and does process below fine work

2.1.1 multipotency corn stem cell is extracted from corn embryo：

Corn embryonic stem cell is present in corn embryo, from maize (1～3 leaf rice of corn of corn nursery 8d During seedling), including cotyledon, plumule, plumular axis, radicle and endosperm, Eradicates is except hoja, all kinds of risk factors and impurity, corn embryo In there is substantial amounts of corn embryonic stem cell, be not required to be kept completely separate out corn embryonic stem cell, therefore one piece of collection, and obtain corn Embryonic stem cell and other maize cells.

2.1.2 corn embryo (rice seedling) is cleaned：

Rinsed repeatedly, shredded with sterile distilled water, add 1：5 sterile distilled water crushes beating, obtains corn embryo slurry, Include corn embryonic stem cell, other corn tissue's cell, string, tissue fluid etc..

2.1.3 embryo's slurry is filtered：

By 200 mesh filters, string and impurity are filtered off, retain corn embryonic stem cell, its hetero-organization of corn thin Born of the same parents and tissue fluid.

2.1.4 the identification of corn stem cell：

The present embodiment adopts genetic test, while the caryogram that also make karyotyping cultured cells to determine is normal, with And carry out internal, external Analytical Chemical Experiment the corn stem cell for obtaining is identified.

3. eggembryosin is extracted from corn embryonic stem cell

3.1 smudge cells：

Various kinds of cell breaking method is had been developed at present, it is broken to adapt to different purposes and different types of cell membrane. Breaking method can be advised to be received as Mechanical Method and the big class of on-mechanical method two.1. Mechanical Method：Including high-pressure homogenization crush method (homogenization), vibrate pearl and hit crush method (Skaking Bead), high-speed stirred pearl grinding crush method (fine Grinding), ultrasonic fragmentation (ultrasonication) etc..2. on-mechanical method：Osmotic shock crush method (osmotic Shock), freeze thawing crush method (freezing and thawing), the molten crush method of enzyme (enzyme lysis), chemically fragmenting method (chemical treatment) and detergent crush method (detergents) etc., according to environmental condition, select respectively.The present invention Corn embryo slurry is adopted into low-temperature and high-speed bead mill method broken wall treatment, including cell membrane and nuclear membrane, embryo's slurry after breaking-wall cell In suspension liquid, i.e. CESC liquid.The method of cell disruption for being adopted can make the worm's ovum suitable with cell dimensions, bacterium while killing Go out.

3.2CESC liquid degreasings：

Fat in cell solution is removed using freezing.

The extraction of 3.3CEF：

Using centrifuge at≤50 DEG C, and under >=5 DEG C of temperature conditionss, 3000r/min or so is centrifuged 15 minutes, under removal Portion's sediment, collects top suspension, and it is Hollow Fiber Ultrafiltrations more than 800000 dalton that suspension is crossed into molecular cut off Device, obtains ultrafiltrate, as CEF liquid, to be detected.Centrifugation is adopted both to can remove other impurities and do not destroyed many in the present embodiment Peptide structure, and more corn eggembryosins can be obtained, operation is also more convenient.Set centrifuge temperature between 5 DEG C -50 DEG C, both The eggembryosin of extraction can be made in liquid, and have larger limit as temperature, it is ensured that the eggembryosin of extraction will not be inactivated, and be preferably set Centrifuge temperature further preferred 5 DEG C -10 DEG C, adopts 5 DEG C at 5 DEG C -30 DEG C in the present embodiment.

The collection of 3.4CEF：

Made according to subsequent product and requirement, the loose agglomerate of off-white color can will be obtained after CEF liquid freeze-dryings i.e. beautiful Rice eggembryosin (solid), or above-mentioned ultrafiltrate Jing is concentrated by ultrafiltration to the CEF of 50% concentration (liquid) packing preservations, it is to be detected.

The detection of 4.CEF

The detection of 4.1 light microscopes：

Using optics or electron microscope observation, free from admixture, it is acellular, see that little particle mixing liquid is the present embodiment and carries Take the corn eggembryosin of preparation.

The detection of 4.2 biochemistry：

1. the measure of protein content：Spectrophotometer measurement method is adopted by kjeldahl nitrogen method, the content of protein is detected；② The identification of molecular weight：Peptide content detection method is carried out using trichloroacetic acid nitrogen solubility index (THA-NSI) to enter suspension content Row detection, below 180～1000 dalton, the material for being small peptide, oligopeptides and polypeptide is CEF to molecular weight.Jing biochemical analysis embryos Tire element main component Co-Q10, nucleic acid, pantothenic acid, lipid, polypeptide, amino acid, Arbutin, bacopa monnieri, lutein, SOD are (super Superoxide dismutase) etc., other compositions need to be studied further.3. Purity：Identified using zone electrophoresis method, concrete operations are shown in Clinical biochemical technology；4. immunological identification：Using double agar diffusion test.

4.3 main component：

Jing biochemical analysises the present embodiment obtain eggembryosin main component be：Co-Q10 accounts for 42%, and amino acid accounts for 6%, EGF (EGF) accounts for 5%, Arbutin, nucleic acid, pantothenic acid, bacopa monnieri, lutein, ERF (epidermis reparative factor), SOD (superoxide dismutase) etc. accounts for 41%, and the purity of corn eggembryosin is more than 98%, and other compositions need to be studied further.

4.4 composition characteristic：

Co-Q10：It is a kind of vitamin, is also a kind of fat-soluble quinone, high-efficiency antioxidant agent can prevents cardiac arrhythmia, mistake Quick disease, angina pectoris, edema, hypertension and elimination superabundant fats.It plays very important effect in terms of anti-aging.Coenzyme Q10 and Vitamin C, E are much like, have helped neutralization.

Pantothenic acid：Vitamin 5 known to us, can potentially control worry, disappointment, anxiety, headache, confirmed fatigue etc., also The sex dysfunction that alcohol or tobacco cause can be helped improve.

Bacopa monnieri：Research shows that bacopa monnieri can support the learning and memory part of brain.Bacopa monnieri has anti-oxidant Property, the region for determining memory can be protected and brain pressure is reduced.Tradition application is it has been shown that bacopa monnieri is to promoting cerebral function to have Direct effect, such as strengthens focusing on and improving memory.

Lutein：Lutein is a kind of important antioxidant.

The sterilization treatment of 5.CEF

5.1 pass through breaking-wall cell method

During broken wall bacterium also rupture of membranes and inactivate.

5.2 Baths spy's sterilization treatment (sterilizing and the poison that goes out)：

60 DEG C, continuous 10h.Thoroughly sterilizing is reached, the activity of corn eggembryosin can be kept again.Generally sterilized using high-temperature Need to sterilize in short-term, less than 6 hours.The present embodiment is carried out disinfection using continuous long-time higher temperature, is fully and completely gone Except virus, while guarantee the biologically active of eggembryosin, it is visible in follow-up clinical test to have given play to efficient biologically active.

The safety examination of 6.CEF

6.1 carry out animal acute toxicity test, animal sensitivity test, rabbit pyrogenic test.

6.2 Bacteria Cultures are checked.

The formulation of 7.CEF is prepared with concentration (content)

7.1 sublimed preparation

7.1.1CEF protecting liver and detoxication tea is red：

In the CEF of the red addition 5% of protecting liver and detoxication tea, CEF protecting liver and detoxications tea is configured to red.

7.1.2CEF heart dredging collateral tea is protected red：

In the CEF for protecting the red addition 5% of heart dredging collateral tea, it is configured to CEF and protects heart dredging collateral tea pellet.

7.1.3CEF the steady sugar tea of pancreas is protected red：

The CEF that the steady sugar tea pellet of pancreas adds 5% is being protected, CEF is being configured to and is protected the steady sugar tea pellet of pancreas.

7.1.4CEF spleen stomach nourishing tea is protected red：

In the CEF for protecting the red addition 5% of spleen stomach nourishing tea, it is configured to CEF and protects spleen stomach nourishing tea pellet.

7.1.5CEF lung oxygenation tea is protected red：

In the CEF for protecting the red addition 5% of lung oxygenation tea, it is configured to CEF and protects lung oxygenation tea pellet.

7.1.6CEF kidney vigor nourishing tea is protected red：

In the CEF for protecting the red addition 5% of kidney vigor nourishing tea, it is configured to CEF and protects kidney vigor nourishing tea pellet.

7.2 medicinal liquor

7.2.1CEF wine：

50 DEG C of rice wine are configured to the CEF wine of 5% concentration.

7.2.2 time Bao Zhilin CEF medicinal liquor：

The time Bao Zhilin CEF wine of 5% concentration is configured to time Bao Zhilin wine.

7.3 oral liquid

7.3.1CEF oral liquid：

The CEF oral liquids of 5% concentration are configured to pure sterile purified water.

7.3.2CEF ferine honey oral liquid：

12% ferine honey liquid, then the CEF ferine honeys for being configured to 5% concentration are first configured to pure sterile purified water Liquid.

7.4 beverage

7.4.1CEF mineral water：

The CEF beverages of 1% concentration are configured to mineral water.

7.4.2CEF beverage：

The CEF of 1% concentration is added in autogamy beverage, CEF beverages are configured to.

7.5 cosmetics

7.5.1CEF keep a public place clean face cream：Face cream of keeping a public place clean in plus 5%CEF, be fabricated to CEF and keep a public place clean face cream.

7.5.2 facial mask

7.5.2.1CEF protect skin moisture saver mask：

Add 5%CEF in skin moisture saver mask is protected, be fabricated to CEF and protect skin moisture saver mask.

7.5.2.2CEF protect skin Deacne pack：

Add 5%CEF in skin Deacne pack is protected, be fabricated to CEF and protect skin Deacne pack.

7.5.2.3CEF protect skin freckle-removing mask：

Add 5%CEF in skin freckle-removing mask is protected, be fabricated to CEF and protect skin freckle-removing mask.

7.5.2.4CEF protecting skin determines spring facial mask：

Determine in spring facial mask plus 5%CEF skin is protected, be fabricated to CEF guarantor's skins and determine spring facial mask.

7.5.3CEF protect skin Essence：

Add 5%CEF in skin Essence is protected, be fabricated to CEF and protect skin Essence.

7.6 externally applied drug

7.6.1CEF spray：

The 10%CEF in the spray of autogamy, is fabricated to CEF sprays.

8. pack

8.1 sublimed preparation are packed

8.1.1CEF protecting liver and detoxication tea is red：

Using vial, 0.3g, 30 pack.

8.1.2CEF heart dredging collateral tea is protected red：

Using vial, 0.3g, 30 pack.

8.1.3CEF the steady sugar tea of pancreas is protected red：

Using vial, 0.3g, 30 pack.

8.1.4CEF spleen stomach nourishing tea is protected red：

Using vial, 0.3g, 30 pack.

8.1.5CEF lung oxygenation tea is protected red：

Using vial, 0.3g, 30 pack.

8.1.6CEF kidney vigor nourishing tea is protected red：

Using vial, 0.3g, 30 pack.

8.2 medicinal liquor are packed

8.2.1CEF medicinal liquor：Adopt

Vial 50ml, ceramic bottle 500ml is used to pack.

8.2.2 time Bao Zhilin CEF medicinal liquor：Using vial 50ml, ceramic bottle 500ml is packed.

8.3 oral liquids are packed

8.3.1CEF oral liquid：

Packed using vial 10ml.

8.3.2CEF ferine honey oral liquid：

Packed using vial 10ml.

8.4 beverage packaging

8.4.1CEF mineral water：

It is canned using plastic bottle 500ml.

8.4.2CEF beverage：

It is canned using iron flask 250ml.

8.5 cosmetic package

8.5.1CEF face cream：

It is canned using 90 grams of vial.

8.5.2 facial mask is packed

8.5.2.1CEF protect skin moisture saver mask：

Using plastic bag packaging.

8.5.2.2CEF protect skin Deacne pack：

Using plastic bag packaging.

8.5.2.3CEF protect skin freckle-removing mask：

Using plastic bag packaging.

8.5.2.4CEF protecting skin determines spring facial mask：

Using plastic bag packaging.

8.5.3CEF protect skin Essence：

Packed using vial 100ml.

8.6CEF spray：

PET bottle 100ml is packed.

9. storage method

The red storage of 9.1 tea

9.1.1CEF protecting liver and detoxication tea is red：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.1.2CEF heart dredging collateral tea is protected red：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.1.3CEF the steady sugar tea of pancreas is protected red：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.1.4CEF spleen stomach nourishing tea is protected red：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.1.5CEF lung oxygenation tea is protected red：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.1.6CEF kidney vigor nourishing tea is protected red：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.2 medicinal liquor are stored

9.2.1CEF medicinal liquor：

Place and deposited 20 years under normal temperature (25 DEG C).

9.2.2 time Bao Zhilin CEF medicinal liquor：

Place and deposited 20 years under normal temperature (25 DEG C).

9.3 oral liquids are stored

9.3.1CEF oral liquid：

Normal temperature is preserved 365 days, is put cool dark place and is preserved.

9.3.2CEF ferine honey oral liquid：

Normal temperature is preserved 365 days, is put cool dark place and is preserved.

9.4 beverages are stored

9.4.1CEF mineral water：

Normal temperature is preserved 365 days, is put cool dark place and is preserved.

9.4.2CEF beverage：

Normal temperature is preserved 365 days, is put cool dark place and is preserved.

9.5 cosmetics are stored

9.5.1CEF face cream：Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 3～4 years.

9.5.2 facial mask is stored

9.5.2.1CEF protect skin moisture saver mask：

Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 2～3 years.

9.5.2.2CEF protect skin Deacne pack：

Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 2～3 years.

9.5.2.3CEF protect skin freckle-removing mask：

Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 2～3 years.

9.5.2.4CEF protecting skin determines spring facial mask：

Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 2～3 years.

9.5.3 essence：

Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 2～3 years.

9.6 externally applied drug

9.6.1CEF spray：Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 2 years.

Embodiment 2

CEF treats zoopery

The model of different wounds and different 15 kinds of diseases is made from big small white mouse, rabbit, dog, monkey, is done with CEF using control More than 500 different zooperies of pre- sequential treatment, model includes the representative disease of 9 kinds of following system：(1) dog head trauma Wound model；(2) monkey cerebral ischemic model；(3) dog Chronic obstructive pulmonary disease；(4) dog taste-blindness rate model；(5) Dog cirrhosis (cirrhosis) model；(6) dog acute myocardial infarction AMI ejector half；(7) dog diabetes model；(8) dog renal failure mould Type；(9) dog bum model (CEF treatment zooperies are present invention innovation)；(10) other models.Each group animal model standard is pressed Requirement of experiment is sought unity of standard；Criterion of therapeutical effect presses relevant disease criterion of therapeutical effect.This research is proved：To 9 kinds of representative diseases of each system Treatment curative effect is preferable, and it the results are shown in Table 1.

Table 1CEF intervenes and compares (X ± S) with 15 kinds of curative effect of disease of conventional therapy

With to group than * P<0.01

Embodiment 3

The clinical practice test of CEF

The disease of 1.CEF treatments：

Embryo's extract for treating is adapted to each systemic disease, including inferior health, promotion Children Normal growth, development, immune system Defective disease, infection disease, tumour (all kinds of innocent and malignant tumours), the nervous system disease, respiratory disease, digestive system disease Disease, disease of cardiovascular system, endocrine system disease, bone and muscle systems disease, disease in the blood system, urinary system disease Disease, genital system diseases, sexual function disease, peripheral artery disease, skin disease, eye, ear, nose, pharynx, larynx difficult disease are anti-ageing Old and beauty etc..

The disease number of cases of 2.CEF treatments：

The example for the treatment of method sequential treatment patient more than 2000 of various diseases and anti-aging.

The therapeutic scheme of 3.CEF：

1. the selection of CEF：The CEF products of different formulations are selected according to different disease degrees.

2. course for the treatment of scheme：Low doses 10 times；The middle course for the treatment of 30 times；The long-range course for the treatment of 60 times.

3. course for the treatment of Scheme Choice：Low doses are applied to anti-aging；The middle course for the treatment of is applied to anti-aging+chronic disease；Long-term therapy Suitable for chronic disease or malignant tumour.

The treatment method of 4.CEF

(CEF protecting liver and detoxications tea is red, CEF protects heart dredging collateral tea pellet, the steady sugar tea of CEF guarantor's pancreases is red, CEF guarantors for 4.1CEF sublimed preparation medicines It is red 6 kinds that spleen stomach nourishing tea is red, CEF protects lung oxygenation tea pellet, CEF protects kidney vigor nourishing tea) instructions of taking：

Low doses：3.0g, orally, 3 times/day, continuous 10d；

The middle course for the treatment of：3.0g, orally, 3 times/day, continuous 30d；

Long-term therapy：3.0g, it is orally, 3 times/day, annual continuous 60 times, continuous 3 years.

4.2CEF medicinal liquor (CEF medicinal liquor, 2 kinds of time Bao Zhilin CEF medicinal liquor) instructions of taking：

Low doses：30ml, orally, 2 times/day, continuous 10d；

The middle course for the treatment of：30ml, orally, 2 times/day, continuous 30d；

Long-term therapy：30ml, it is orally, 2 times/day, annual continuous 60 times, continuous 3 years.

4.3CEF oral liquids (CEF oral liquids, 2 kinds of CEF ferine honeys oral liquid) instructions of taking：

Low doses：10ml, orally, 2 times/day, continuous 10d；

The middle course for the treatment of：10ml, orally, 2 times/day, continuous 30d；

Long-term therapy：10ml, it is orally, 2 times/day, annual continuous 60 times, continuous 3 years.

4.4CEF beverages (2 kinds of CEF mineral waters, CEF beverages) drinking method：

Low doses：1 bottle (500ml, 250ml), orally, 2 times/day, continuous 10d；

The middle course for the treatment of：10ml, orally, 2 times/day, continuous 30d；

Long-term therapy：10ml, it is orally, 2 times/day, annual continuous 60 times, continuous 3 years.

4.5CEF face cream application processes：

After face cleaning outward apply use, 2 times/day, can prolonged application, this research using 30 days observe curative effect.

4.6CEF spray application processes：

Spray after surface of a wound debridement, 2 times/day, till healing.

(CEF protects skin moisture saver mask, CEF guarantor's skin Deacne packs, CEF guarantor's skin freckle-removing masks, CEF and protects skin and determine 4.7CEF facial masks 4 kinds of spring facial mask), 1 times/day, can prolonged application.

The therapeutic effect of 5.CEF

5.1 apply CEF series of products symptom situations of change

More than 2000 example patient statisticses are treated all effectively according to this technology, when changing using CEF series of products symptom Between table 2.

Using CEF series of products symptom transformation periods table 2

5.2CEF series of products are intervened and 20 kinds of disease patient's comparitive studies of conventional therapy

The different example of various diseases more than 2000 of each system of sequential treatment of the present invention, main 50 kinds of each system code disease is faced Bed Ureteral Calculus the results are shown in Table 2.Each group disease presses diagnostic criteria, and criterion of therapeutical effect presses relevant disease criterion of therapeutical effect.Each systemic disease, 3 are shown in Table using the intervention of CEF series of products and 20 kinds of conventional therapy disease patient's comparitive study its curative effect.

Table 3CEF series of products are intervened and 20 kinds of disease patient's comparitive studies (X ± S) of conventional therapy

With to group than * P<0.01

5.3 other diseases do not compare test more than 500, efficient more than 98%.It is shown in Table 4.

Table 4CEF interventions do not compare test disease patient's curative effect

Other diseases more than 500, it is efficient more than 98%.

The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (9)

1. a kind of preparation method of corn eggembryosin, it is characterised in that comprise the following steps：

(1) elite corn kind；

(2) nursery, is dried prior to seeding for 8 days, is soaked 2～3 days with 1% limewash after drying 1～2 day, then uses water Immersion 4～5 days, vernalization 8 days；

(3) collect corn embryo and obtain corn embryonic stem cell；

(4) breaking-wall cell process is carried out to corn embryonic stem cell, carries out impurity elimination process successively afterwards, centrifugal treating obtains jade Rice pluripotent embryonic element；

The corn embryonic stem cell is multipotency corn embryonic stem cell.

2. the preparation method of corn eggembryosin as claimed in claim 1, it is characterised in that in the step (3), by what is collected Shred after corn embryo cleaning, add and crush after 3～5 times of distilled water beating, obtain the corn containing corn embryonic stem cell Embryo starches, then obtains corn embryonic stem cell after 200 mesh are filtered.

3. the preparation method of corn eggembryosin as claimed in claim 1, it is characterised in that in the step (4), the cell breaks Using low-temperature and high-speed bead mill method by corn embryonic stem cell broken wall, the centrifugal treating is the temperature strip at 5 DEG C -50 DEG C for wall process Under part, 3000r/min is centrifuged 10-15 minutes.

4. the preparation method of corn eggembryosin as claimed in claim 1, it is characterised in that also include the corn eggembryosin to obtaining Sterilization treatment is carried out, 10 hours are continued at a temperature of 60 DEG C.

5. a kind of corn eggembryosin, it is characterised in that obtained using the preparation method as described in claim 1-4 is arbitrary.

6. corn eggembryosin as claimed in claim 5, it is characterised in that including 42% Co-Q10,6% amino acid, 5% EGF, 41% Arbutin, nucleic acid, pantothenic acid, bacopa monnieri, lutein, epidermis reparative factor, super oxygen Thing mutase, the purity of the corn eggembryosin is more than 98%.

7. a kind of purposes of corn eggembryosin as claimed in claim 6, it is characterised in that for preparing treatment cardiovascular and cerebrovascular, sugar Urine disease, gastroduodenal ulcer, hepatitis, the medicine of the chronic disease of renal failure and anti-aging and beautification product.

8. the purposes of corn eggembryosin as claimed in claim 7, it is characterised in that the formulation of the medicine or product includes oral Pill, oral liquid, external application emulsion, it is administered to medicament.

9. as described in claim 6-8 is arbitrary corn eggembryosin purposes, it is characterised in that the corn eggembryosin is preparing medicine The 1%-12% of unit dose drug or product is accounted in thing or product.