CN106620311A

CN106620311A - Preparation method and application of millet embryo extract

Info

Publication number: CN106620311A
Application number: CN201610942070.8A
Authority: CN
Inventors: 谢培增; 沈会强; 刘江; 谢成; 沈明阳; 郑晓云
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-10-26
Filing date: 2016-10-26
Publication date: 2017-05-10

Abstract

The invention discloses a preparation method of a millet embryo extract. The preparation method comprises the following steps: conducting cell wall-breaking treatment on millet embryo stem cells, removing impurities and conducting centrifuging, so that the pluripotent millet embryo extract is obtained. The method also discloses the millet embryo extract prepared by the preparation method and an application of the millet embryo extract. The pluripotent millet embryo extract, which is isolated from the pluripotent millet embryo stem cells, can achieve effects of a human embryo extract, namely keeping activities of various cells in a human body and prolonging service life of working cells; repairing injured cells and recovering cell functions; activating dormant cells in the human body and supplementing effective working cells; protecting attenuation of autologous stem cells and promoting generation of the stem cells; and exciting differentiation of autologous dormant stem cells to generate new cells to substitute for dead cells, so that a function of recovering organs is achieved.

Description

Broomcorn millet eggembryosin preparation method and application

Technical field

The present invention relates to drug medication, food and cosmetics technical field, especially a kind of broomcorn millet eggembryosin, specifically addresses this The preparation method of broomcorn millet eggembryosin and application.

Background technology

Although human diseases has thousands of kinds, but real disease only has one kind, that is, cytopathy, any one organ Cell metabolism it is out of joint, cell death reaches to a certain degree, then this organ just can it is sick, cause cell failure Main reasons is that two aspects：One is cytotrophy, oxygen supply is not enough, and two is that metabolic waste can not in time be discharged and cause cell The reason for caused by poisoning, and the present understanding to disease is the performance of disease, rather than real, mostly controls to the ill in treatment Treat (such as pain, the process of heating) or to treatment-resistant (excision to tumour, Radiotherapy chemotherapy etc.), most diseases are all right Disease is controlled.This research is to go out new functioning cell using stem cell division in this group of biomolecule human activin of broomcorn millet eggembryosin to substitute damage Wound and dead cell, and recover and normal function, reach the purpose for fundamentally treating disease.

1912, Switzerland scientist karr professor had found to have in sheep embryonic cell first and a kind of can make what cell rejuvenated Magical material -- sheep embryo extract；1931, the mode of Ni Han professor's first passage injections was by the parathyroid cells application of lamb Yu Yiwei impaired patients In danger of parathyroid gland in operation have saved its life；Nineteen thirty-seven, professor Ni Han for the first time will be living thin Born of the same parents' therapy is applied to brain nervous cell, after, living cells therapy is extended to liver, pancreas, kidney, the heart by professor Ni Han Dirty, duodenum, thymus gland and spleen.Because sheep embryo extract is from the embryonic cell of sheep, there are all many-sides not with human cell Together, there are many contraindications, such as over anaphylaxis crowd during clinical practice, hyperthyroidism, serious hyperpietic are pregnant Phase women's use, tumor patient etc. can not be applied.

Because fresh and alive cell can not be preserved for a long time, the scientist of 1949 Nian Nihan professors and Nestle company assists jointly Make, lyophilized living cells is developed first carries out cell activation treatment；Nineteen fifty-five Switzerland Dr.Alfred doctors of medicine Pfister portion Divide the defect for (freezing or freezing) damage and deteriorating course and the Techniques of preserving for causing when solving cell extraction.

Martin Evans in 1970 are isolated from mouse blastular go out mouse embryo stem cell with vitro culture first (embryonic stem cell, ESCs, abbreviation ES, EK or ESC cell.)；American scientist vitro culture of human embryo in 1998 Stem cell success, hereafter using the different disease of different stem-cell therapies, its short effect can agree Chinese scholars It is fixed, but presently, there are problems with：Either sheep placenta or embryonic stem cell, due to being that xenogenesis (sheep) is all existed with allosome Polluted bacteria causes the severe complications such as general infection when immunological rejection, allergy, in vitro culture.

1980, because fresh and alive sheep embryo extract lacks the strict antibacterial measure of sterilization, it is impossible to long-term to preserve, there is infection Dangerous very big, Switzerland's medical circle is devoted to always development purification, and sterilization is sterilized, Techniques of preserving, but still can not reach long-time The purpose of preservation.

Nineteen eighty-three, Edward doctor Bei Chi inherits the achievement of Buddhist nun's writing brush sheep embryo extract active therapy, develops personalization Treatment technology, purifies " the full organ essence " for small molecule, more easily absorbs；Help human body activation and newly to bear structure complete It is whole, the active neoblast of function；Replace due to aging sick cell caused by various cause noxa element institutes, strengthen each life system Function, recovers human body energy and muscle power, reaches more significantly anti-aging curative effect.

American scientist vitro culture of human embryo's stem cell success in 1998.

Hereafter, using the different disease of different stem-cell therapies, its short effect can be affirmed Chinese scholars, But presently, there are problems with：Either sheep placenta or human embryo stem cell, due to being xenogenesis (sheep) and variant cell, after use Polluted bacteria causes the severe complications such as general infection when patient has immunological rejection, allergy, in vitro culture.People's embryo Tire stem cell also has ethics and legal issue.These problems are avoided, over nearly more than 160 years, scientist is dreamed of from plant Plant stem cell is isolated in separate living tissue or forming layer, although doing many painstaking efforts, is but never obtained into Work(, or even it is believed that this is impossible mythology.

2005, Korea cloud fire science and technology institute was successfully separated first in the world and has cultivated plant stem cell, and by its It is named as many Bels (Ddobyul).Research has shown that plant stem cell has powerful anti-oxidant and activity of fighting against senium, can be significantly Improve body immunity and anti-cancer ability.

Plant stem cell (Plant stem cell) includes all formulas with regard to development of plants and growth, possesses forever The cell (immortal cell) of permanent vitality, is the root (origin) of plant life power.Plant stem cell is present in and is claimed For in merismatic special tectonic, with very surprising power of regeneration.These allow plant continuous between centuries Growth, and generate brand-new organ.The research in stem cell clinical testing application has shown that stem cell animal can break up becomes group Knit cell, stem cell animal repair damaged tissues it was determined that but plant stem cell is huge with stem cell animal difference, plant is dry Cell can only break up becomes other kinds of plant cell, absolutely not to differentiate human cell.So, directly using plant The damage that stem cell is used to repair human tissue cells is unpractical.

The content of the invention

The present invention is directed to the deficiencies in the prior art, because plant stem cell cannot be directly used to repair the damage of human cell, So the effective ingredient of plant stem cell whether can the division of activation of human autologous stem cells realizing repairing and supplement human tissue Cell, reaches the purpose of rehabilitationThe present inventor proposes a kind of broomcorn millet eggembryosin preparation method and application, and proves plant embryos Tire element can repair the stem cell division of resting state in the cell of body injury and activation body, produce new cell replacement dead Cell, and play its effect, reach the effect of fundamentally disease preventing and treating, and easy to operate, effect extensively, so as to solve This technical barrier.

Broomcorn millet (Classification system：Panicum miliaceum, English name：Millet)) also known as small Semen setariae, broom corn millet, summer millet. For grass family Panicum annual herb.Broomcorn millet has 7670～7610 years away from modern, and the soil of north of China is all broomcorn millets " ancestral home ". Its son is real to boil rear toughness, can make wine, cook cake etc..It is sweet, it is warm in nature, it is nontoxic, it is more bitter.Cure mainly beneficial gas, bowl spares.Modern science Developing has shown that rice sprout research, the B family vitamins in broomcorn millet, vitamin E, carrotene, niacin, folic acid, pantothenic acid, amino acid etc. Multiclass nutriment, extracts its composition as medicinal.

The present invention provides technical scheme below：

A kind of preparation method of broomcorn millet eggembryosin, comprises the following steps：

(1) selected broomcorn millet kind；

(2) nursery, is dried prior to seeding for 8 days, dry 1～2 day after with 1% limewash immersion 2～3 days, then It is soaked in water 4～5 days, vernalization 8 days；

(3) collect broomcorn millet embryo and obtain broomcorn millet embryonic stem cell；

(4) breaking-wall cell process is carried out to broomcorn millet embryonic stem cell, carries out impurity elimination process successively afterwards, centrifugal treating is obtained Broomcorn millet pluripotent embryonic element；

The broomcorn millet embryonic stem cell is multipotency broomcorn millet embryonic stem cell.

Preferably, in the step (3), shred after the broomcorn millet embryo for collecting is cleaned, after adding 3～5 times of distilled water Beating is crushed, the slurry of the broomcorn millet embryo containing broomcorn millet embryonic stem cell is obtained, then broomcorn millet embryonic stem cell is obtained after 200 mesh are filtered.

Preferably, in the step (4), the breaking-wall cell is processed and adopts low-temperature and high-speed bead mill method by broomcorn millet embryonic stem cell Broken wall, the centrifugal treating is the 3000r/min under 5 DEG C -50 DEG C of temperature conditionss, and 10-15 minutes are centrifuged.

Preferably, also include that the broomcorn millet eggembryosin to obtaining carries out sterilization treatment, 10 hours are continued at a temperature of 60 DEG C.

The present invention also provides a kind of broomcorn millet eggembryosin, is obtained using above-mentioned preparation method.

Preferably, the broomcorn millet eggembryosin include 42% Co-Q10,6% amino acid, 5% EGF, 41% lignan, forulic acid, alkyl-resorcin, Arbutin, nucleic acid, pantothenic acid, carrotene, bacopa monnieri, epidermis repair because Son, superoxide dismutase, the purity of the broomcorn millet eggembryosin is more than 98%.

The present invention also provides a kind of purposes of broomcorn millet eggembryosin, for preparing treatment cardiovascular and cerebrovascular, diabetes, gastroduodenal Ulcer, hepatitis, the medicine of the chronic disease of renal failure and anti-aging and beautification product.

Preferably, the formulation of the medicine includes oral administration pills, oral liquid, external application emulsion, is administered to medicament.

Preferably, the broomcorn millet eggembryosin accounts for the 1%-12% of unit dose drug or product in medicine or product is prepared. Compared with prior art, the present invention has advantages below：The isolated broomcorn millet pluripotent embryonic element mixture from broomcorn millet embryonic stem cell (mostly small-molecular peptides) are by oral administration or local topical can directly absorb.MEF is entered can activate itself dormancy stem cell point after human body The dead cell of new cell substitute is dissolved, the effect for restoring internal organs is reached, it both makees more than the treatment of current stem cell transplantation With, and without the complication being likely to occur after stem cell transplantation, such as immunological rejection, medication way is levied convenience, safety, can also be avoided The problem of Human embryo element ethics and law, is fundamentally to prevent and treat all kinds of, various diseases, and especially chronic, difficult disease resists Aging, prolongation life-span, beauty etc. are optimal natural Health preservation therapies.

Specific embodiment

Describe the present invention with reference to embodiment, the description of this part be only it is exemplary and explanatory, no Reply protection scope of the present invention has any restriction effect.

The present invention is by extracting from broomcorn millet embryonic stem cell (millet embryonic stem cells, MESC) Broomcorn millet eggembryosin (millet embryo factor, MEF), is currently viewed as all kinds of incurable disease diseases, especially for preventing and treating It is all kinds of chronic, difficult diseases and anti-aging and beauty.

The action principle of MEF：Alternative Human embryos element (HEF) of MEF, can keep cell viability, extend the longevity of working cardial cell Life；Damaging cells are repaired, recovers the function of cell；Extend the life-span of internal stem cell；Internal rest cell is activated, supplements effective Working cardial cell；Itself dormancy stem cell is activated, breaks up different tissues cell, to substitute the thin of aging, damage and death is updated Born of the same parents, restore histoorgan, reach source radical curing of disease, reverse the effect in youth.

Broomcorn millet embryonic stem cell, in being present in broomcorn millet embryo, from the broomcorn millet nursery broomcorn millet embryo of the 8th day (during 1～3 leaf rice seedling of broomcorn millet), bag Enclosed tool leaf, plumule, plumular axis, radicle and endosperm, Eradicates removes wheat skin, all kinds of risk factors and impurity, there is substantial amounts of broomcorn millet in broomcorn millet embryo Embryonic stem cell, is not required to be kept completely separate out broomcorn millet embryonic stem cell, therefore one piece of collection, and other are thin to obtain broomcorn millet embryonic stem cell and broomcorn millet Born of the same parents.

Broomcorn millet embryo (rice seedling) is cleaned, rinsed repeatedly, shredded with sterile distilled water, by 1：5 ratio adds aseptic distillation The broken beating of pigment, obtains broomcorn millet embryo slurry, the composition such as embryonic stem cell containing broomcorn millet, broomcorn millet other histocytes, string, tissue fluid, Cross elimination string, retain the compositions such as broomcorn millet embryonic stem cell, broomcorn millet other histocytes, tissue fluid.

Broomcorn millet embryonic stem cell adopts low-temperature and high-speed bead mill method broken wall treatment, including cell membrane and nucleus mould, while can make Worm's ovum, the bacterium suitable with cell dimensions also can be killed, most after Jing centrifugation after obtain broomcorn millet eggembryosin.

Broomcorn millet eggembryosin of the present invention is pluripotent embryonic element.

Due to taking in 8 days in broomcorn millet embryo, its overwhelming majority is pluripotent embryonic stem cells to MEF of the present invention, so obtain Eggembryosin is pluripotent embryonic element, and its effect can keep cell viability, extend the life-span of working cardial cell；Damaging cells are repaired, it is extensive The function of multiple cell；Activate internal rest cell, the effective working cardial cell of supplement；The decay and promotion of protection autologous stem cells is dry Hemapoiesis；Activate itself dormancy stem cell and differentiate various types of cells, dead cell of substituting reaches the effect for restoring internal organs, For treating various diseases, especially chronic or difficult disease is very effective with anti-aging curative effect, the product can preserve for a long time, It is non-toxic, without rejection, medication orally with external application, convenience, safety, can also avoid the problem of Human embryo element ethics and law.

Embodiment 1

The present embodiment provides a kind of preparation method of broomcorn millet eggembryosin

1. broomcorn millet embryonic tissue is obtained

1.1 selected broomcorn millet kinds：

Select the requirement of seed rice：It is preferred from high yield, health is disease-free, seed rice then.The present embodiment is military from inner mongolia River county broomcorn millet Seed-breeding base obtains high-quality, high yield, disease-resistant hybridization broomcorn millet combination (kind).Different, the seed rice quantity according to output demand It is different, can be with 10,100,500 kilograms as a radix, according to test with experimental measuring with 500 kilograms of broomcorn millets as a radix.

1.2 correct nursery：

Prior to seeding 8d is basked seeds, and to be shone and soak 2～3d with 1% limewash after 1～2d, is beneficial to disinfection, is promoted Enter seed sprouting.Then cleaned with clear water and use again 4～6d of cold water soak, make seed fully absorb water, note changing water and stirring, finally Vernalization 8d is filtered, it is seen that when germinal layer, plumule, plumular axis, radicle and endosperm, embryo seedling about 1.0cm or so, as broomcorn millet embryo.

1.3 cleaning embryo seedlings：

When collecting broomcorn millet embryo, by clean operation, subsequently embryo seedling 30min is soaked with sterile pure water, Eradicates removes all kinds of risk factors, Such as impurity, rinse repeatedly and inserted in sterile bag after broomcorn millet embryonic tissue.

2. stem cell is obtained from broomcorn millet embryo

Embryo is extracted in 2.1 broomcorn millet embryos and does process below fine work

2.1.1 multipotency broomcorn millet stem cell is extracted from broomcorn millet embryo：

Broomcorn millet embryonic stem cell is present in broomcorn millet embryo, from the broomcorn millet embryo (during 1～3 leaf rice seedling of broomcorn millet) of broomcorn millet nursery 8d, including Cotyledon, plumule, plumular axis, radicle and endosperm, Eradicates removes rice husk, all kinds of risk factors and impurity, there is substantial amounts of broomcorn millet embryo in broomcorn millet embryo Tire stem cell, is not required to be kept completely separate out broomcorn millet embryonic stem cell, therefore one piece is collected, and it is thin to obtain broomcorn millet embryonic stem cell and other broomcorn millets Born of the same parents.

2.1.2 broomcorn millet embryo (rice seedling) is cleaned：

Rinsed repeatedly, shredded with sterile distilled water, add 1：5 sterile distilled water crushes beating, obtains broomcorn millet embryo slurry, interior Embryonic stem cell containing broomcorn millet, other broomcorn millet histocyte, string, tissue fluid etc..

2.1.3 embryo's slurry is filtered：

By 200 mesh filters, filter off string and impurity, retain broomcorn millet embryonic stem cell, other histocytes of broomcorn millet and Tissue fluid.

2.1.4 the identification of broomcorn millet stem cell：

The present embodiment adopts genetic test, while the caryogram that also make karyotyping cultured cells to determine is normal, with And carry out internal, external Analytical Chemical Experiment the broomcorn millet stem cell for obtaining is identified.

3. eggembryosin is extracted from broomcorn millet embryonic stem cell

3.1 smudge cells：

Various kinds of cell breaking method is had been developed at present, it is broken to adapt to different purposes and different types of cell membrane. Breaking method can be advised to be received as Mechanical Method and the big class of on-mechanical method two.1. Mechanical Method：Including high-pressure homogenization crush method (homogenization), vibrate pearl and hit crush method (Skaking Bead), high-speed stirred pearl grinding crush method (fine Grinding), ultrasonic fragmentation (ultrasonication) etc..2. on-mechanical method：Osmotic shock crush method (osmotic Shock), freeze thawing crush method (freezing and thawing), the molten crush method of enzyme (enzyme lysis), chemically fragmenting method (chemical treatment) and detergent crush method (detergents) etc., according to environmental condition, select respectively.The present invention Broomcorn millet embryo slurry is adopted into low-temperature and high-speed bead mill method broken wall treatment, including cell membrane and nuclear membrane, embryo's slurry is in after breaking-wall cell Suspension liquid, i.e. MESC liquid.The method of cell disruption for being adopted can make the worm's ovum suitable with cell dimensions, bacterium while killing Go out.

3.2 MESC liquid degreasings：

Fat in cell solution is removed using freezing.

The extraction of 3.3 MEF：

Using centrifuge at≤50 DEG C, and under >=5 DEG C of temperature conditionss, 3000r/min or so is centrifuged 15 minutes, under removal Portion's sediment, collects top suspension, and it is Hollow Fiber Ultrafiltrations more than 800000 dalton that suspension is crossed into molecular cut off Device, obtains ultrafiltrate, as MEF liquid, to be detected.Centrifugation is adopted both to can remove other impurities and do not destroyed many in the present embodiment Peptide structure, and more eggembryosins can be obtained, operation is also more convenient.Setting centrifuge temperature can be between 5 DEG C -50 DEG C, both The eggembryosin of extraction is made in liquid, and has larger limit as temperature, it is ensured that the eggembryosin of extraction will not be inactivated, and preferably set centrifugation Machine temperature further preferred 5 DEG C -10 DEG C, adopts 5 DEG C at 5 DEG C -30 DEG C in the present embodiment.

The collection of 3.4 MEF：

Made according to subsequent product and requirement, can will obtain the loose agglomerate of off-white color i.e. broomcorn millet after MEF liquid freeze-dryings Eggembryosin (solid), or above-mentioned ultrafiltrate Jing is concentrated by ultrafiltration to the MEF of 50% concentration (liquid) packing preservations, it is to be detected.

The detection of 4.MEF

The detection of 4.1 light microscopes：

Using optics or electron microscope observation, free from admixture, it is acellular, see that little particle mixing liquid is the present embodiment and carries Take the broomcorn millet eggembryosin of preparation.

The detection of 4.2 biochemistry：

1. the measure of protein content：An Shi nitrogen method adopts spectrophotometer measurement method, detects the content of protein；2. divide The identification of son amount：Carry out peptide content detection method using trichloroacetic acid nitrogen solubility index (THA-NSI) is carried out to suspension content Detection, below 180～1000 dalton, the material for being small peptide, oligopeptides and polypeptide is MEF to molecular weight.Jing biochemical analysis embryos Plain main component Co-Q10, nucleic acid, pantothenic acid, lipid, polypeptide, amino acid, lignan, forulic acid, alkyl-resorcin, false horse Bitterroot, SOD (superoxide dismutase) etc., other compositions need to be studied further.3. Purity：Reflected using zone electrophoresis method Fixed, clinical biochemical technology is shown in concrete operations；4. immunological identification：Using double agar diffusion test.

4.3 main component：

Jing biochemical analysises the present embodiment extract broomcorn millet eggembryosin main component be：Co-Q10 accounts for 42%, amino acid 6%, EGF (EGF) accounts for 5%, and lignan, forulic acid, alkyl-resorcin, Arbutin, nucleic acid, pantothenic acid, carrotene is false Purslane, ERF (epidermis reparative factor), SOD (superoxide dismutase) etc. 41%, the purity of eggembryosin is more than 98%, its He need to further study composition.

4.4 composition characteristic：

Co-Q10：It is a kind of vitamin, is also a kind of fat-soluble quinone, high-efficiency antioxidant agent can prevents cardiac arrhythmia, mistake Quick disease, angina pectoris, edema, hypertension and elimination superabundant fats.It plays very important effect in terms of anti-aging.Coenzyme Q10 and Vitamin C, E are much like, have helped neutralization.

Pantothenic acid：Vitamin 5 known to us, can potentially control worry, disappointment, anxiety, headache, confirmed fatigue etc., also The sex dysfunction that alcohol or tobacco cause can be helped improve.

Bacopa monnieri：Research shows that bacopa monnieri can support the learning and memory part of brain.Bacopa monnieri has anti-oxidant Property, the region for determining memory can be protected and brain pressure is reduced.Tradition application is it has been shown that bacopa monnieri is to promoting cerebral function to have Direct effect, such as strengthens focusing on and improving memory.

Carrotene：After intake human digestive organ, vitamin A can be changed into.

The sterilization treatment of 5.MEF

5.1 pass through breaking-wall cell method

During broken wall bacterium also rupture of membranes and inactivate.

5.2 Baths spy's sterilization treatment (sterilizing and the poison that goes out)：

60 DEG C, continuous 10h.Thoroughly sterilizing is reached, the activity of broomcorn millet eggembryosin can be kept again.Generally needed using high-temperature sterilization Sterilize in short-term, less than 6 hours.The present embodiment is carried out disinfection using continuous long-time higher temperature, is fully and completely removed Virus, while guarantee the biologically active of eggembryosin, it is visible in follow-up clinical test to have given play to efficient biologically active.

The safety examination of 6.MEF

6.1 carry out animal acute toxicity test, animal sensitivity test, rabbit pyrogenic test.

6.2 Bacteria Cultures are checked.

The formulation of 7.MEF is prepared with concentration (content)

The broomcorn millet eggembryosin accounts for the 1%-12% of unit dose drug in medicine or product is prepared.The UD is The percentage composition of contained eggembryosin in the amount of compounding pharmaceutical or product least unit, the percentage of eggembryosin contains as contained by a red ball Amount, the percentage concentration of eggembryosin contained by one bottle of medicinal liquor, the percentage composition of eggembryosin contained by a piece of facial mask.

7.1 sublimed preparation

7.1.1 MEF protecting liver and detoxications tea is red：

In the MEF of the red addition 5% of protecting liver and detoxication tea, MEF protecting liver and detoxications tea is configured to red.

7.1.2 MEF protects heart dredging collateral tea pellet：

In the MEF for protecting the red addition 5% of heart dredging collateral tea, it is configured to MEF and protects heart dredging collateral tea pellet.

7.1.3 MEF protects the steady sugar tea pellet of pancreas：

The MEF that the steady sugar tea pellet of pancreas adds 5% is being protected, MEF is being configured to and is protected the steady sugar tea pellet of pancreas.

7.1.4 MEF protects spleen stomach nourishing tea pellet：

In the MEF for protecting the red addition 5% of spleen stomach nourishing tea, it is configured to MEF and protects spleen stomach nourishing tea pellet.

7.1.5 MEF protects lung oxygenation tea pellet：

In the MEF for protecting the red addition 5% of lung oxygenation tea, it is configured to MEF and protects lung oxygenation tea pellet.

7.1.6 MEF protects kidney vigor nourishing tea pellet：

In the MEF for protecting the red addition 5% of kidney vigor nourishing tea, it is configured to MEF and protects kidney vigor nourishing tea pellet.

7.2 medicinal liquor

7.2.1 MEF medicinal liquor：

50 DEG C of rice wine are configured to the MEF wine of 5% concentration.

7.2.2 time Bao Zhilin MEF medicinal liquor：

The time Bao Zhilin MEF wine of 5% concentration is configured to time Bao Zhilin wine.

7.3 oral liquid

7.3.1 MEF oral liquids：

The MEF oral liquids of 5% concentration are configured to pure sterile purified water.

7.3.2 MEF ferine honey oral liquids：

12% ferine honey liquid, then the MEF ferine honeys for being configured to 5% concentration are first configured to pure sterile purified water Liquid.

7.4 beverage

7.4.1 MEF mineral waters：

The MEF beverages of 1% concentration are configured to mineral water.

7.4.2 MEF beverages：

The MEF of 1% concentration is added in autogamy beverage, MEF beverages are configured to.

7.5 cosmetics

The face cream 7.5.1 MEF keeps a public place clean：

Face cream of keeping a public place clean in plus 5%MEF, be fabricated to MEF and keep a public place clean face cream.

7.5.2 facial mask

7.5.2.1 MEF protects skin moisture saver mask：

Add 5%MEF in skin moisture saver mask is protected, be fabricated to MEF and protect skin moisture saver mask.

7.5.2.2 MEF protects skin Deacne pack：

Add 5%MEF in skin Deacne pack is protected, be fabricated to MEF and protect skin Deacne pack.

7.5.2.3 MEF protects skin freckle-removing mask：

Add 5%MEF in skin freckle-removing mask is protected, be fabricated to MEF and protect skin freckle-removing mask.

7.5.2.4 MEF guarantors skin determines spring facial mask：

Determine in spring facial mask plus 5%MEF skin is protected, be fabricated to MEF guarantor's skins and determine spring facial mask.

7.5.3 MEF protects skin Essence：

Add 5%MEF in skin Essence is protected, be fabricated to MEF and protect skin Essence.

7.6 externally applied drug

7.6.1 MEF sprays：

The 10%MEF in the spray of autogamy, is fabricated to MEF sprays.

8. pack

8.1 sublimed preparation are packed

8.1.1 MEF protecting liver and detoxications tea is red：

Using vial, 0.3g, 30 pack.

8.1.2 MEF protects heart dredging collateral tea pellet：

Using vial, 0.3g, 30 pack.

8.1.3 MEF protects the steady sugar tea pellet of pancreas：

Using vial, 0.3g, 30 pack.

8.1.4 MEF protects spleen stomach nourishing tea pellet：

Using vial, 0.3g, 30 pack.

8.1.5 MEF protects lung oxygenation tea pellet：

Using vial, 0.3g, 30 pack.

8.1.6 MEF protects kidney vigor nourishing tea pellet：

Using vial, 0.3g, 30 pack.

8.2 medicinal liquor are packed

8.2.1 MEF medicinal liquor：

Using vial 50ml, ceramic bottle 500ml is packed.

8.2.2 time Bao Zhilin MEF medicinal liquor：

Using vial 50ml, ceramic bottle 500ml is packed.

8.3 oral liquids are packed

8.3.1 MEF oral liquids：

Packed using vial 10ml.

8.3.2 MEF ferine honey oral liquids：

Packed using vial 10ml.

8.4 beverage packaging

8.4.1 MEF mineral waters：

It is canned using plastic bottle 500ml.

8.4.2 MEF beverages：

It is canned using iron flask 250ml.

8.5 cosmetic package

8.5.1 MEF face cream：

It is canned using 90 grams of vial.

8.5.2 facial mask is packed

8.5.2.1 MEF protects skin moisture saver mask：

Using plastic bag packaging.

8.5.2.2 MEF protects skin Deacne pack：

Using plastic bag packaging.

8.5.2.3 MEF protects skin freckle-removing mask：

Using plastic bag packaging.

8.5.3 MEF protects skin Essence：

Packed using vial 100ml.

8.6 MEF sprays：

PET bottle 100ml is packed.

9. storage method

9.1 the red storage of tea

9.1.1 MEF protecting liver and detoxications tea is red：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.1.2 MEF protects heart dredging collateral tea pellet：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.1.3 MEF protects the steady sugar tea pellet of pancreas：

Less than 5 DEG C are placed, is preserved more than 1 year.

It is red that 9.1.4 MEF protects spleen stomach nourishing tea：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.1.5 MEF protects lung oxygenation tea pellet：

Less than 5 DEG C are placed, is preserved more than 1 year.

It is red that 9.1.6 MEF protects kidney vigor nourishing tea：

Less than 5 DEG C are placed, is preserved more than 1 year.

9.2 medicinal liquor are stored

9.2.1 MEF medicinal liquor：

Place and deposited 20 years under normal temperature (25 DEG C).

9.2.2 time Bao Zhilin MEF medicinal liquor：

Place and deposited 20 years under normal temperature (25 DEG C).

9.3 oral liquids are stored

9.3.1 MEF oral liquids：

Normal temperature is preserved 365 days, is put cool dark place and is preserved.

9.3.2 MEF ferine honey oral liquids：

9.4 beverages are stored

9.4.1 MEF beverages：

9.4.2 MEF beverages：

9.5 cosmetics are stored

9.5.1 MEF face cream：

Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 3～4 years.

9.5.2 facial mask is stored

9.5.2.1 MEF protects skin moisture saver mask：

Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 2～3 years.

9.5.2.2 MEF protects skin Deacne pack：

9.5.2.3 MEF protects skin freckle-removing mask：

9.5.2.4 MEF guarantors skin determines spring facial mask：

9.5.3 essence：

9.6 externally applied drug

9.6.1 MEF sprays：

Normal temperature is preserved 365 days, is put cool dark place and is preserved, 0～4 DEG C 2 years.

Embodiment 2

MEF treats zoopery

The model of different wounds and different 15 kinds of diseases is made from big small white mouse, rabbit, dog, monkey, is done with REF using control More than 500 different zooperies of pre- sequential treatment, model includes the representative disease of 9 kinds of following system：(1) dog head trauma Wound model；(2) monkey cerebral ischemic model；(3) dog Chronic obstructive pulmonary disease；(4) dog taste-blindness rate model；(5) Dog cirrhosis (cirrhosis) model；(6) dog acute myocardial infarction AMI ejector half；(7) dog diabetes model；(8) dog renal failure mould Type；(9) dog bum model (REF treatment zooperies are present invention innovation)；(10) other models.Each group animal model standard is pressed Requirement of experiment is sought unity of standard；Criterion of therapeutical effect presses relevant disease criterion of therapeutical effect.This research is proved：To 9 kinds of representative diseases of each system Treatment curative effect is preferable, and it the results are shown in Table 1.

The MEF of table 1 intervenes and compares (X ± S) with 15 kinds of curative effect of disease of conventional therapy

With to group than * P<0.01

Embodiment 3

The clinical practice test of MEF

The disease of 1.MEF treatments：

Embryo's extract for treating is adapted to each systemic disease, including inferior health, promotion Children Normal growth, development, immune system Defective disease, infection disease, tumour (all kinds of innocent and malignant tumours), the nervous system disease, respiratory disease, digestive system disease Disease, disease of cardiovascular system, endocrine system disease, bone and muscle systems disease, disease in the blood system, urinary system disease Disease, genital system diseases, sexual function disease, peripheral artery disease, skin disease, eye, ear, nose, pharynx, larynx difficult disease are anti-ageing Old and beauty etc..

The disease number of cases of 2.MEF treatments：

The example for the treatment of method sequential treatment patient more than 2000 of various diseases and anti-aging.

The therapeutic scheme of 3.MEF：

1. the selection of MEF：The MEF products of different formulations are selected according to different disease degrees.

2. course for the treatment of scheme：Low doses 10 times；The middle course for the treatment of 30 times；The long-range course for the treatment of 60 times.

3. course for the treatment of scheme：Low doses are applied to anti-aging；The middle course for the treatment of is applied to anti-aging+chronic disease；Long-term therapy is suitable for In chronic disease or malignant tumour.

The treatment method of 4.MEF

(MEF protecting liver and detoxications tea is red, MEF protects heart dredging collateral tea pellet, the steady sugar tea of MEF guarantor's pancreases is red, MEF guarantors for 4.1 MEF sublimed preparation medicines It is red 6 kinds that spleen stomach nourishing tea is red, MEF protects lung oxygenation tea pellet, MEF protects kidney vigor nourishing tea) instructions of taking：

Low doses：3.0g, orally, 3 times/day, continuous 10d；

The middle course for the treatment of：3.0g, orally, 3 times/day, continuous 30d；

Long-term therapy：3.0g, it is orally, 3 times/day, annual continuous 60 times, continuous 3 years.

4.2 MEF medicinal liquor (MEF medicinal liquor, 2 kinds of time Bao Zhilin MEF medicinal liquor) instructions of taking：

Low doses：30ml, orally, 2 times/day, continuous 10d；

The middle course for the treatment of：30ml, orally, 2 times/day, continuous 30d；

Long-term therapy：30ml, it is orally, 2 times/day, annual continuous 60 times, continuous 3 years.

4.3 MEF oral liquids (MEF oral liquids, 2 kinds of MEF ferine honeys oral liquid) instructions of taking：

Low doses：10ml, orally, 2 times/day, continuous 10d；

The middle course for the treatment of：10ml, orally, 2 times/day, continuous 30d；

Long-term therapy：10ml, it is orally, 2 times/day, annual continuous 60 times, continuous 3 years.

4.4 MEF beverages (2 kinds of MEF mineral waters, MEF beverages) drinking method：

Low doses：1 bottle (500ml, 250ml), orally, 2 times/day, continuous 10d；

4.5 MEF face cream application processes：

After face cleaning outward apply use, 2 times/day, can prolonged application, this research using 30 days observe curative effect.

4.6 MEF spray application processes：

Spray after surface of a wound debridement, 2 times/day, till healing.

(MEF protects skin moisture saver mask, MEF guarantor's skin Deacne packs, MEF guarantor's skin freckle-removing masks, MEF and protects skin and determine 4.7 MEF facial masks 4 kinds of spring facial mask), 1 times/day, can prolonged application.

The therapeutic effect of 5.MEF

5.1 apply MEF series of products symptom situations of change

More than 2000 example patient statisticses are treated all effectively according to this technology, when changing using MEF series of products symptom Between table 2.

Using MEF series of products symptom transformation periods table 2

5.2 MEF series of products are intervened and 20 kinds of disease patient's comparitive studies of conventional therapy

The different example of various diseases more than 2000 of each system of sequential treatment of the present invention, main 50 kinds of each system code disease is faced Bed Ureteral Calculus the results are shown in Table 2.Each group disease presses diagnostic criteria, and criterion of therapeutical effect presses relevant disease criterion of therapeutical effect.Each systemic disease, 3 are shown in Table using the intervention of MEF series of products and 20 kinds of conventional therapy disease patient's comparitive study its curative effect.

The MEF series of products of table 3 are intervened and 20 kinds of disease patient's comparitive studies (X ± S) of conventional therapy

With to group than * P<0.01

5.3 other diseases do not compare test more than 500, efficient more than 98%.It is shown in Table 4.

The MEF of table 4 interventions do not compare test disease patient's curative effect

Other diseases more than 500, it is efficient more than 98%.

The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims

1. a kind of preparation method of broomcorn millet eggembryosin, it is characterised in that comprise the following steps：

(1) selected broomcorn millet kind；

(2) nursery, is dried prior to seeding for 8 days, is soaked 2～3 days with 1% limewash after drying 1～2 day, then uses water Immersion 4～5 days, vernalization 8 days；

(4) breaking-wall cell process is carried out to broomcorn millet embryonic stem cell, carries out impurity elimination process successively afterwards, centrifugal treating obtains broomcorn millet many Can eggembryosin；

2. the preparation method of broomcorn millet eggembryosin as claimed in claim 1, it is characterised in that in the step (3), by the broomcorn millet collected Shred after embryo's cleaning, add and crush after 3～5 times of distilled water beating, obtain the slurry of the broomcorn millet embryo containing broomcorn millet embryonic stem cell, then Broomcorn millet embryonic stem cell is obtained after 200 mesh are filtered.

3. the preparation method of broomcorn millet eggembryosin as claimed in claim 1, it is characterised in that in the step (4), the breaking-wall cell Process broomcorn millet embryonic stem cell broken wall using low-temperature and high-speed bead mill method, the centrifugal treating is the temperature conditionss at 5 DEG C -50 DEG C Under, 3000r/min is centrifuged 10-15 minutes.

4. the preparation method of broomcorn millet eggembryosin as claimed in claim 1, it is characterised in that also include that the broomcorn millet eggembryosin to obtaining is carried out Sterilization treatment, continues 10 hours at a temperature of 60 DEG C.

5. a kind of broomcorn millet eggembryosin, it is characterised in that obtained using the preparation method as described in claim 1-4 is arbitrary.

6. broomcorn millet eggembryosin as claimed in claim 5, it is characterised in that including 42% Co-Q10,6% amino acid, 5% EGF, 41% lignan, forulic acid, alkyl-resorcin, Arbutin, nucleic acid, pantothenic acid, carrotene, false horse Bitterroot, epidermis reparative factor, superoxide dismutase, the purity of the broomcorn millet eggembryosin is more than 98%.

7. a kind of purposes of broomcorn millet eggembryosin as claimed in claim 6, it is characterised in that for preparing treatment cardiovascular and cerebrovascular, glycosuria Disease, gastroduodenal ulcer, hepatitis, the medicine of the chronic disease of renal failure and anti-aging and beautification product.

8. the purposes of broomcorn millet eggembryosin as claimed in claim 7, it is characterised in that the formulation of the medicine or product includes oral ball Agent, oral liquid, external application emulsion, it is administered to medicament.

9. as described in claim 6 to 8 is arbitrary broomcorn millet eggembryosin purposes, it is characterised in that the broomcorn millet eggembryosin is preparing medicine Or account for the 1%-12% of unit dose drug or product in product.