CN113736863A - 诊断方法和组合物 - Google Patents

诊断方法和组合物 Download PDF

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Publication number
CN113736863A
CN113736863A CN202110943909.0A CN202110943909A CN113736863A CN 113736863 A CN113736863 A CN 113736863A CN 202110943909 A CN202110943909 A CN 202110943909A CN 113736863 A CN113736863 A CN 113736863A
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CN
China
Prior art keywords
primer
amplification reaction
region
complementary
nucleic acid
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Pending
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CN202110943909.0A
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English (en)
Chinese (zh)
Inventor
P·帕特尔
I·吴
A·理查德森
K·贝尔霍钦
J·李
S·塔巴克曼
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Theranos Inc
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Theranos Inc
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Priority claimed from PCT/US2014/056151 external-priority patent/WO2015076919A1/en
Application filed by Theranos Inc filed Critical Theranos Inc
Publication of CN113736863A publication Critical patent/CN113736863A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202110943909.0A 2014-09-17 2015-09-17 诊断方法和组合物 Pending CN113736863A (zh)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201462051912P 2014-09-17 2014-09-17
US201462051945P 2014-09-17 2014-09-17
USPCT/US2014/056151 2014-09-17
US62/051,945 2014-09-17
US62/051,912 2014-09-17
PCT/US2014/056151 WO2015076919A1 (en) 2013-11-22 2014-09-17 Nucleic Acid Amplification
US201462068603P 2014-10-24 2014-10-24
US201462068605P 2014-10-24 2014-10-24
US62/068,605 2014-10-24
US62/068,603 2014-10-24
US201562151358P 2015-04-22 2015-04-22
US62/151,358 2015-04-22
CN201580062485.XA CN108026591B (zh) 2014-09-17 2015-09-17 诊断方法和组合物

Related Parent Applications (1)

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CN201580062485.XA Division CN108026591B (zh) 2014-09-17 2015-09-17 诊断方法和组合物

Publications (1)

Publication Number Publication Date
CN113736863A true CN113736863A (zh) 2021-12-03

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CN201580061362.4A Pending CN107002066A (zh) 2014-09-17 2015-09-17 复合式多步核酸扩增
CN202110943909.0A Pending CN113736863A (zh) 2014-09-17 2015-09-17 诊断方法和组合物

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CN201580061362.4A Pending CN107002066A (zh) 2014-09-17 2015-09-17 复合式多步核酸扩增

Country Status (8)

Country Link
US (3) US9909193B2 (enExample)
EP (2) EP3194629A4 (enExample)
JP (6) JP2017532030A (enExample)
CN (3) CN108026591B (enExample)
BR (1) BR112017005392A2 (enExample)
CA (2) CA2960859A1 (enExample)
MX (2) MX2017003473A (enExample)
WO (2) WO2016044673A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108026591B (zh) 2014-09-17 2021-09-07 赛拉诺斯知识产权有限责任公司 诊断方法和组合物
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
BR112019012057A2 (pt) 2016-12-15 2019-11-12 Meharry Medical College agente antiviral, composição farmacêutica para a administração do mesmo e métodos de redução na replicação do vírus zika (zikv) e tratamento de doença mediada pelo vírus zika (zikv)
FI130673B1 (en) * 2020-11-24 2024-01-11 Mobidiag Oy Method and system for performing a cumulative nucleic acid amplification reaction

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US20090263789A1 (en) * 1998-06-24 2009-10-22 Enzo Life Sciences, Inc. Method for Detecting Nucleic Acid Sequences
CN102242211A (zh) * 2011-07-08 2011-11-16 无锡锐奇基因生物科技有限公司 检测突变核酸序列的方法及试剂盒
AU2010251184A1 (en) * 2009-05-19 2011-11-24 Sentinel Ch S.P.A. A dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications
US20130224746A1 (en) * 1996-05-29 2013-08-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
WO2014025337A1 (en) * 2012-08-06 2014-02-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Methods and reagents for amplifying nucleic acids

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US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
EP1319718A1 (en) * 2001-12-14 2003-06-18 Keygene N.V. High throughput analysis and detection of multiple target sequences
WO2005014810A1 (ja) * 2003-08-08 2005-02-17 National Institute Of Advanced Industrial Science And Technology ダンベル型ｄｎａの効率的な製造方法
JP4379087B2 (ja) * 2003-11-07 2009-12-09 トヨタ自動車株式会社 ゲラニルゲラニル二リン酸合成酵素及びゲラニルゲラニオールの製造方法
WO2005095654A1 (en) * 2004-03-25 2005-10-13 Biohelix Corporation Helicase-dependent amplification of circular nucleic acids
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US20090263789A1 (en) * 1998-06-24 2009-10-22 Enzo Life Sciences, Inc. Method for Detecting Nucleic Acid Sequences
US20020081598A1 (en) * 2001-04-09 2002-06-27 Evans William Edward Haplotyping method for multiple distal nucleotide polymorphisms
CN101321877A (zh) * 2005-10-03 2008-12-10 阿普里拉股份有限公司 用于扩增核酸的组合物、方法和试剂盒
AU2010251184A1 (en) * 2009-05-19 2011-11-24 Sentinel Ch S.P.A. A dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications
CN102242211A (zh) * 2011-07-08 2011-11-16 无锡锐奇基因生物科技有限公司 检测突变核酸序列的方法及试剂盒
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Publication number Publication date
CA2960859A1 (en) 2016-03-24
JP2017531424A (ja) 2017-10-26
MX366113B (es) 2019-06-26
JP2017532030A (ja) 2017-11-02
CN107002066A (zh) 2017-08-01
EP3194590A4 (en) 2018-05-09
US20160201148A1 (en) 2016-07-14
EP3194590A1 (en) 2017-07-26
JP2022110152A (ja) 2022-07-28
US20170342476A1 (en) 2017-11-30
MX2017003473A (es) 2017-08-02
JP2021006056A (ja) 2021-01-21
CN108026591B (zh) 2021-09-07
WO2016044664A1 (en) 2016-03-24
WO2016044673A1 (en) 2016-03-24
BR112017005392A2 (pt) 2017-12-12
CN108026591A (zh) 2018-05-11
JP2021000138A (ja) 2021-01-07
JP2023011943A (ja) 2023-01-24
EP3194629A1 (en) 2017-07-26
CA2961209A1 (en) 2016-03-24
MX2017003475A (es) 2017-08-02
EP3194629A4 (en) 2018-03-07
US20180223381A1 (en) 2018-08-09
US9909193B2 (en) 2018-03-06

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Application publication date: 20211203