CA2960859A1 - Hybrid multi-step nucleic acid amplification - Google Patents

Hybrid multi-step nucleic acid amplification Download PDF

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Publication number
CA2960859A1
CA2960859A1 CA2960859A CA2960859A CA2960859A1 CA 2960859 A1 CA2960859 A1 CA 2960859A1 CA 2960859 A CA2960859 A CA 2960859A CA 2960859 A CA2960859 A CA 2960859A CA 2960859 A1 CA2960859 A1 CA 2960859A1
Authority
CA
Canada
Prior art keywords
nucleic acid
primer
sample
amplification
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA2960859A
Other languages
English (en)
French (fr)
Inventor
Pranav Patel
Indira WU
Aaron RICHARDSON
Kamila BELHOCINE
Josephine Lee
Scott TABAKMAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Labrador Diagnostics LLC
Original Assignee
Theranos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2014/056151 external-priority patent/WO2015076919A1/en
Application filed by Theranos Inc filed Critical Theranos Inc
Publication of CA2960859A1 publication Critical patent/CA2960859A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/706Specific hybridization probes for hepatitis
    • C12Q1/707Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/706Specific hybridization probes for hepatitis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2960859A 2014-09-17 2015-09-17 Hybrid multi-step nucleic acid amplification Pending CA2960859A1 (en)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201462051912P 2014-09-17 2014-09-17
US201462051945P 2014-09-17 2014-09-17
PCT/US2014/056151 WO2015076919A1 (en) 2013-11-22 2014-09-17 Nucleic Acid Amplification
US62/051,912 2014-09-17
US62/051,945 2014-09-17
USPCT/US2014/056151 2014-09-17
US201462068603P 2014-10-24 2014-10-24
US201462068605P 2014-10-24 2014-10-24
US62/068,603 2014-10-24
US62/068,605 2014-10-24
US201562151358P 2015-04-22 2015-04-22
US62/151,358 2015-04-22
PCT/US2015/050822 WO2016044673A1 (en) 2014-09-17 2015-09-17 Hybrid multi-step nucleic acid amplification

Publications (1)

Publication Number Publication Date
CA2960859A1 true CA2960859A1 (en) 2016-03-24

Family

ID=55533878

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2961209A Pending CA2961209A1 (en) 2014-09-17 2015-09-17 Diagnostic methods and compositions
CA2960859A Pending CA2960859A1 (en) 2014-09-17 2015-09-17 Hybrid multi-step nucleic acid amplification

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA2961209A Pending CA2961209A1 (en) 2014-09-17 2015-09-17 Diagnostic methods and compositions

Country Status (8)

Country Link
US (3) US9909193B2 (enExample)
EP (2) EP3194629A4 (enExample)
JP (6) JP2017532030A (enExample)
CN (3) CN113736863A (enExample)
BR (1) BR112017005392A2 (enExample)
CA (2) CA2961209A1 (enExample)
MX (2) MX366113B (enExample)
WO (2) WO2016044673A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2961209A1 (en) 2014-09-17 2016-03-24 Theranos, Inc. Diagnostic methods and compositions
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
CN110462042B (zh) 2016-12-15 2023-10-27 梅哈里医学院 抗病毒剂
FI130673B1 (en) * 2020-11-24 2024-01-11 Mobidiag Oy Method and system for performing a cumulative nucleic acid amplification reaction

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0912761A4 (en) * 1996-05-29 2004-06-09 Cornell Res Foundation Inc DETERMINATION OF DIFFERENCES IN THE NUCLEIC ACID SEQUENCE BY MEANS OF A COMBINATION OF LIGASE DETERMINATION AND POLYMERASE CHAIN REACTION
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
AU2001244677A1 (en) * 2000-04-07 2001-10-23 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7041447B2 (en) * 2001-04-09 2006-05-09 St. Jude Children's Hospital, Inc. Haplotyping method for multiple distal nucleotide polymorphisms
EP1319718A1 (en) * 2001-12-14 2003-06-18 Keygene N.V. High throughput analysis and detection of multiple target sequences
WO2005014810A1 (ja) * 2003-08-08 2005-02-17 National Institute Of Advanced Industrial Science And Technology ダンベル型dnaの効率的な製造方法
JP4379087B2 (ja) * 2003-11-07 2009-12-09 トヨタ自動車株式会社 ゲラニルゲラニル二リン酸合成酵素及びゲラニルゲラニオールの製造方法
US20100075384A1 (en) * 2004-03-25 2010-03-25 New England Biolabs, Inc. Helicase-dependent amplification of circular nucleic acids
WO2006003721A1 (en) * 2004-07-02 2006-01-12 Kabushiki Kaisha Dnaform Method for preparing sequence tags
EP2458011B1 (en) * 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
EP2162555A4 (en) * 2007-06-22 2011-02-23 Univ Columbia SPECIFIC AMPLIFICATION OF TUMOR-SPECIFIC DNA SEQUENCES
MX352987B (es) 2007-10-02 2017-12-15 Theranos Ip Co Llc Dispositivos modulares de punto de cuidado y usos de los mismos.
WO2010127020A1 (en) * 2009-04-29 2010-11-04 Js Genetics Inc. Molecular diagnosis of fragile x syndrome associated with fmr1 gene
IT1394539B1 (it) * 2009-05-19 2012-07-05 Sentinel Ch S P A Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso
WO2012038049A2 (en) * 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
CN102242211A (zh) * 2011-07-08 2011-11-16 无锡锐奇基因生物科技有限公司 检测突变核酸序列的方法及试剂盒
US8380541B1 (en) 2011-09-25 2013-02-19 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
CN108490204A (zh) 2011-09-25 2018-09-04 赛拉诺斯知识产权有限责任公司 用于多重分析的系统和方法
US9809845B2 (en) * 2012-08-06 2017-11-07 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods and reagents for amplifying nucleic acids
JP2014140367A (ja) * 2012-12-27 2014-08-07 Tosoh Corp B型肝炎ウイルスdna増幅用プライマーおよびそれを用いたb型肝炎ウイルスdna検出法
SG11201507272RA (en) 2013-03-15 2015-10-29 Theranos Inc Nuclei acid amplification
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Also Published As

Publication number Publication date
BR112017005392A2 (pt) 2017-12-12
JP2023011943A (ja) 2023-01-24
US9909193B2 (en) 2018-03-06
WO2016044664A1 (en) 2016-03-24
US20180223381A1 (en) 2018-08-09
CN108026591A (zh) 2018-05-11
CA2961209A1 (en) 2016-03-24
JP2017531424A (ja) 2017-10-26
EP3194629A1 (en) 2017-07-26
MX2017003475A (es) 2017-08-02
MX2017003473A (es) 2017-08-02
EP3194590A4 (en) 2018-05-09
CN107002066A (zh) 2017-08-01
CN108026591B (zh) 2021-09-07
MX366113B (es) 2019-06-26
US20170342476A1 (en) 2017-11-30
JP2021006056A (ja) 2021-01-21
CN113736863A (zh) 2021-12-03
JP2017532030A (ja) 2017-11-02
US20160201148A1 (en) 2016-07-14
JP2021000138A (ja) 2021-01-07
EP3194590A1 (en) 2017-07-26
WO2016044673A1 (en) 2016-03-24
JP2022110152A (ja) 2022-07-28
EP3194629A4 (en) 2018-03-07

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