CN113736101B - 一种利用酵母菌发酵制备多功能水凝胶的方法 - Google Patents
一种利用酵母菌发酵制备多功能水凝胶的方法 Download PDFInfo
- Publication number
- CN113736101B CN113736101B CN202111038363.0A CN202111038363A CN113736101B CN 113736101 B CN113736101 B CN 113736101B CN 202111038363 A CN202111038363 A CN 202111038363A CN 113736101 B CN113736101 B CN 113736101B
- Authority
- CN
- China
- Prior art keywords
- pca
- yeast
- solution
- hydrogel
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000017 hydrogel Substances 0.000 title claims abstract description 71
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 title claims abstract description 19
- 230000004151 fermentation Effects 0.000 title claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 51
- 238000003756 stirring Methods 0.000 claims abstract description 36
- 239000008103 glucose Substances 0.000 claims abstract description 29
- 239000011259 mixed solution Substances 0.000 claims abstract description 24
- 239000000243 solution Substances 0.000 claims abstract description 22
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 17
- 108010010803 Gelatin Proteins 0.000 claims abstract description 14
- 239000008273 gelatin Substances 0.000 claims abstract description 14
- 229920000159 gelatin Polymers 0.000 claims abstract description 14
- 235000019322 gelatine Nutrition 0.000 claims abstract description 14
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 14
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 26
- 239000001967 plate count agar Substances 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 23
- 239000008367 deionised water Substances 0.000 claims description 18
- 229910021641 deionized water Inorganic materials 0.000 claims description 18
- 238000001816 cooling Methods 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 17
- 238000005303 weighing Methods 0.000 claims description 15
- 239000012266 salt solution Substances 0.000 claims description 13
- 239000006185 dispersion Substances 0.000 claims description 12
- CTENFNNZBMHDDG-UHFFFAOYSA-N Dopamine hydrochloride Chemical compound Cl.NCCC1=CC=C(O)C(O)=C1 CTENFNNZBMHDDG-UHFFFAOYSA-N 0.000 claims description 11
- 229960001149 dopamine hydrochloride Drugs 0.000 claims description 11
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 7
- 238000009835 boiling Methods 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000001509 sodium citrate Substances 0.000 claims description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000000499 gel Substances 0.000 claims description 3
- 229910002804 graphite Inorganic materials 0.000 claims description 3
- 239000010439 graphite Substances 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 235000010344 sodium nitrate Nutrition 0.000 claims description 3
- 239000004317 sodium nitrate Substances 0.000 claims description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 3
- 238000009210 therapy by ultrasound Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 2
- 230000003213 activating effect Effects 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 claims description 2
- 239000004020 conductor Substances 0.000 claims description 2
- 238000005520 cutting process Methods 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims 1
- 238000007710 freezing Methods 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 5
- 230000035699 permeability Effects 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000005580 one pot reaction Methods 0.000 abstract 1
- 229920001690 polydopamine Polymers 0.000 abstract 1
- 235000011187 glycerol Nutrition 0.000 description 7
- 206010016807 Fluid retention Diseases 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000012876 topography Methods 0.000 description 4
- 230000003183 myoelectrical effect Effects 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- BXNRKCXZILSQHE-UHFFFAOYSA-N propane-1,2,3-triol;sulfuric acid Chemical compound OS(O)(=O)=O.OCC(O)CO BXNRKCXZILSQHE-UHFFFAOYSA-N 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical group [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 2
- 241000282412 Homo Species 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000001132 ultrasonic dispersion Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
- A61B5/25—Bioelectric electrodes therefor
- A61B5/251—Means for maintaining electrode contact with the body
- A61B5/257—Means for maintaining electrode contact with the body using adhesive means, e.g. adhesive pads or tapes
- A61B5/259—Means for maintaining electrode contact with the body using adhesive means, e.g. adhesive pads or tapes using conductive adhesive means, e.g. gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0002—Galenical forms characterised by the drug release technique; Application systems commanded by energy
- A61K9/0009—Galenical forms characterised by the drug release technique; Application systems commanded by energy involving or responsive to electricity, magnetism or acoustic waves; Galenical aspects of sonophoresis, iontophoresis, electroporation or electroosmosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/60—Liquid-swellable gel-forming materials, e.g. super-absorbents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0038—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J9/00—Adhesives characterised by their physical nature or the effects produced, e.g. glue sticks
- C09J9/02—Electrically-conducting adhesives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/12—Agar-agar; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2489/00—Characterised by the use of proteins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/02—Elements
- C08K3/04—Carbon
- C08K3/042—Graphene or derivatives, e.g. graphene oxides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Dispersion Chemistry (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Pharmacology & Pharmacy (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Surgery (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Botany (AREA)
- Zoology (AREA)
- Dermatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Carbon And Carbon Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
本发明涉及一种利用酵母菌发酵制备多功能水凝胶的方法。本发明首先利用聚多巴胺还原氧化石墨烯得到还原氧化石墨烯溶液,然后配制一定浓度的明胶PCA葡萄糖混合溶液以及活化的酵母菌溶液;通过一锅反应法将三者混合,搅拌均匀,倒入模具中,在30℃水浴中发酵一定的时间,即得Gel‑PrGO‑PCA‑酵母多功能水凝胶材料。该方法简单方便、快速高效,所得水凝胶具有良好的透气性、超强的机械性能、导电性、生物相容性,并且可通过贴敷于不同的皮肤位置检测心电和肌电。本发明为制备多孔透气导电水凝胶电极提供一种新的思路及方法,有助于导电水凝胶材料的开发利用,以便其应用在生物传感器领域。
Description
技术领域
本发明属于导电水凝胶的制备领域,具体涉及一种利用酵母菌发酵制备多功能水凝胶的方法。
背景技术
水凝胶是一类具有三维聚合物或超分子聚合物网络结构的材料,具有很好的柔性,可以进行拉、压、弯曲等,其中导电水凝胶是一种通过添加导电高分子聚合物进行物理交联制备的水凝胶。它们独特的性质(如柔韧性、高含水量、生物相容性、导电性等)促使它们广泛应用于各种生物医学领域,包括人体生理信号的检测、再生医学、神经修复等领域。然而,导电水凝胶的透气性、机械性以及保水性差,大大限制了导电水凝胶在生物医用材料领域的应用。因此提高水凝胶的透气性、机械性能以及保水性具有重要意义。
生物传感器是一种重要的检测和追踪人体生理信号的设备,其中水凝胶是一种新型的生物传感器。对于检测和追踪人体的心电、肌电、脑电以及神经信号具有较高的舒适性、形状可控性和灵敏性,目前的水凝胶大多存在不透气、机械强度差等问题,因此制备一种多孔透气高强度的导电水凝胶具有广大的发展和应用前景。并且目前有报道的导电水凝胶的制备含有生物不相容的合成高分子、有毒的交联剂、复杂的操作流程,因此,开发一种简单、快速、安全、高效、透气的方法具有重要意义。
发明内容
本发明的目的在于针对上述领域研究的不足,提供一种利用酵母菌发酵制备多功能水凝胶的方法。该方法操作简单、快速、高效,且所得水凝胶具有良好的透气性、保水性、柔韧性和导电性。
为实现上述目的,采用以下技术方案:
一种利用酵母菌发酵制备多功能水凝的方法,包括如下步骤:
(1)将酵母菌在30℃的热水中活化得到酵母菌液;
(2)称取平板计数琼脂(PCA)溶解在100℃去离子水中煮沸20 min,冷却至50℃,得PCA溶液;向PCA溶液中加入明胶(Gel)于50℃水浴中搅拌30 min,然后加入葡萄糖,继续搅拌溶解得到Gel-PCA-葡萄糖的混合溶液,降温至30℃;
(3)将步骤(1)的酵母菌液和步骤(2)的Gel-PCA-葡萄糖的混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10 min即得到Gel-PCA-酵母菌多功能水凝胶。
上述步骤(1)中所述酵母菌液浓度为0.2 g/mL-0.45 g/mL。
上述步骤(2)中所述PCA溶液的浓度为0.0235 g/mL;明胶的添加量为5 wt%-35wt%;所述的葡萄糖的添加量为0.01-0.06 g/mL。
进一步的,上述步骤(2)中向PCA溶液中加入浓度为1-4 mg/mL的还原氧化石墨烯(PrGO);得到Gel-PrGO-PCA-葡萄糖混合溶液;将步骤(1)的酵母菌液和Gel-PrGO-PCA-葡萄糖混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10min即得到Gel-PrGO-PCA-酵母多功能水凝胶。
更进一步的,所述还原氧化石墨烯(PrGO)的制备方法包括:
(1)氧化石墨烯(GO)的制备:称取1.2 g石墨,加入50 mL 浓硫酸,搅拌均匀后,放入冰浴中,搅拌状态下加入1.5 g硝酸钠,并缓慢加入6 g高锰酸钾,于35℃下搅拌过夜,缓慢加入去离子水100 mL,控制温度为90℃,反应1 h,将30 vol%的过氧化氢稀释5倍后缓慢加入上述溶液中,直到不产生气泡,停止添加,继续反应3 h,冷却至室温,水洗至中性后超声分散20 min,冻干后得到氧化石墨烯(GO)粉末;
(2)称取20 mg GO粉末,加入2.5 mL去离子水,超声直至完全溶解,得到GO分散液;称取50 mg 盐酸多巴胺(DA)粉末溶解在2.5 mL 10 mM Tris-HCl溶液(pH=8.5)中,得盐酸多巴胺分散液;然后将盐酸多巴胺分散液加入到 GO分散液中,冰浴下超声分散2 h后,在60℃水浴下搅拌12 h,得到还原氧化石墨烯PrGO溶液。
进一步的,上述步骤(3)中所得Gel-PCA-酵母菌水凝胶放在-80℃冷冻20 min后切成1 mm厚的薄片或切成任意形状,浸泡在盐溶液和甘油的混合溶液中12 h。所述盐溶液为硫酸铵或柠檬酸钠溶液;所述的盐溶液浓度为10 wt%-30 wt%;所述盐溶液与甘油的体积比为2:1、1:1或1:2。
上述任一项方法制备所得的多功能水凝胶。
上述多功能水凝胶作为导电材料用于生物传感器中,或用于载药、抗菌伤口敷料中。
相对于现有技术,本发明具有以下优点:
(1)本发明采用明胶与还原氧化石墨烯、PCA、酵母菌、葡萄糖混合后进行发酵制备,然后将其浸泡在盐溶液或盐溶液与甘油的混合溶液中。
(2)该利用酵母菌发酵制备的多功能水凝胶具有多种功能,其中酵母菌赋予了其多孔透气性,明胶、PrGO和盐溶液赋予了其导电性和机械性能。
(3)该水凝胶具有抗疲劳性能和超强的拉伸性能,其拉伸性能可达到1000%,并且能灵敏的检测心电和肌电信号,有望应用于生物传感器或可穿戴设备等领域。
附图说明
图1 添加不同含量的明胶对孔径的影响。
图2 水凝胶形貌图。a,普通相机下未添加酵母菌的水凝胶的形貌图;b,光学显微镜下未添加酵母菌的水凝胶形貌图;c,普通相机下添加酵母菌的水凝胶的形貌图;d,光学显微镜下添加酵母菌的水凝胶形貌图。
图3 Gel-PCA-酵母菌-硫酸铵水凝胶和Gel-PCA-酵母菌-柠檬酸钠水凝胶浸泡不同盐溶液后的拉伸应力-应变曲线。
图4 杨氏模量图。
图5 Gel-PrGO-PCA-酵母菌水凝胶的形貌、导电性以及对心电和肌电信号的检测图。a,Gel-PrGO-PCA-酵母菌水凝胶的形貌;b,导电性检测;c,心电信号的检测;d,肌电信号的检测。
图6 Gel-PCA-酵母菌和Gel-PCA-酵母菌-硫酸铵-甘油水凝胶的保水性能。
图7 Gel-PCA-酵母菌和Gel-PCA-酵母菌-硫酸铵-甘油水凝胶的流变性能。
具体实施方式
以下结合实施实例对本发明做进一步说明,需要指出的是,本实施实例仅用于解释本发明,而非对本发明的限制。
实施例 1
称取7份0.235g平板计数琼脂(PCA)分别溶解在9 mL 100℃去离子水中煮沸20min,冷却至50℃,分别加入5 wt%、10 wt%、15 wt%、20 wt%、25 wt%、30 wt%和35 wt%明胶(Gel)于50℃水浴中搅拌30 min,然后加入0.15 g葡萄糖,继续搅拌溶解得到Gel-PCA和葡萄糖的混合溶液,降温至30℃;称取0.45 g 酵母粉溶解于1 mL 30℃去离子水中,然后将酵母菌液和上述Gel-PCA-葡萄糖的混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10 min即得到Gel-PCA-酵母菌水凝胶。最后将其放在-80℃冷冻20 min后切成1 mm厚的薄片或切成任意形状,浸泡在20 wt%的硫酸铵和甘油(体积比1:1)的混合溶液中12 h后进行检测。如图1所示,得到的Gel-PCA-酵母菌水凝胶随着明胶含量的增加,水凝胶的孔径先减小后增加。
实施例 2
将0.235 g PCA溶解在9 mL 100℃去离子水中煮沸20 min,冷却至50℃,加入20wt%明胶于50℃水浴中搅拌30 min,然后加入0.15 g葡萄糖,继续搅拌溶解得到Gel-PCA和葡萄糖的混合溶液,降温至30℃;称取0.45 g 酵母粉溶解于1 mL 30℃去离子水中(对照组不添加酵母菌),然后将酵母菌液和上述Gel-PCA-葡萄糖的混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10 min即得到Gel-PCA-酵母菌水凝胶。最后将其放在-80℃冷冻20 min后切成1 mm厚的薄片或切成任意形状进行检测。如图2所示,得到的Gel-PCA-酵母菌水凝胶具有较好的网络多孔结构。
实施例 3
将0.235 g PCA溶解在9 mL 100℃去离子水中煮沸20 min,冷却至50℃,加入20wt%明胶于50℃水浴中搅拌30 min,然后加入0.15 g葡萄糖,继续搅拌溶解得到Gel -PCA和葡萄糖的混合溶液,降温至40℃;称取0.45 g 酵母粉溶解于1 mL 30℃去离子水中;然后将酵母菌液和上述Gel -PCA和葡萄糖的混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10 min即得到Gel-PCA-酵母菌水凝胶。切成所需要的形状后,将其分别浸泡到10 wt%、20 wt% 和30 wt%的硫酸铵或者柠檬酸钠的盐溶液中12 h后,进行拉伸性能检测。如图3所示,20%盐溶液浸泡组得到的Gel-PCA-酵母菌-硫酸铵水凝胶和Gel-PCA-酵母菌-柠檬酸钠水凝胶具有超强的拉伸性能,其中Gel-PCA-酵母菌-柠檬酸钠水凝胶最大拉伸应变达到1000%,最大拉伸应力为0.28 MPa;Gel-PCA-酵母菌-硫酸铵水凝胶最大拉伸应变达到850%,最大拉伸应力为0.14 MPa,虽然柠檬酸钠组的拉伸性能大于硫酸铵组,但是硫酸铵组的杨氏模量小于柠檬酸钠组的杨氏模量(图4),表明其柔性或弹性较好。10%盐溶液浸泡组的水凝胶的力学性能较差,拉伸性能小于100%。
实施例 4
一种利用酵母菌发酵制备多功能水凝胶的方法,包括以下 步骤:
(1)氧化石墨烯(GO)的制备:称取1.2 g石墨,加入50 mL 浓硫酸,搅拌均匀后,放入冰浴中,搅拌状态下加入1.5 g硝酸钠,并缓慢加入6 g高锰酸钾,于35℃下搅拌过夜,缓慢加入去离子水100 mL,控制温度为90℃,反应1 h,将30 vol%的过氧化氢稀释5倍后缓慢加入上述溶液中,直到不产生气泡,停止添加,继续反应3 h,冷却至室温,水洗至中性后超声分散20 min,冻干后得到GO粉末。
(2)称取20 mg GO粉末,加入2.5 mL去离子水,超声直至完全溶解,得到GO分散液;称取50 mg 盐酸多巴胺(DA)粉末溶解在2.5 mL 10 mM Tris-HCl溶液(pH=8.5)中,得盐酸多巴胺分散液;然后将盐酸多巴胺分散液加入到 GO分散液中,冰浴下超声分散2 h后,在60℃水浴下搅拌12 h,得到还原氧化石墨烯PrGO溶液。
(3)将0.235 g PCA溶解在4 mL 100℃去离子水中,煮沸20 min,冷却至50℃,与步骤(2)制备的PrGO溶液混匀后,加入20 wt%明胶于50℃水浴中搅拌30 min,然后加入0.15 g的葡萄糖,继续搅拌溶解得到Gel-PrGO-PCA-葡萄糖的混合溶液,降温至30℃。称取0.45 g酵母粉溶解于1 mL 30℃去离子水中,得酵母菌液。然后将酵母菌液和上述Gel-PrGO-PCA-葡萄糖的混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10 min即得到Gel-PrGO-PCA-酵母菌水凝胶。如图5所示,得到的Gel-PrGO-PCA-酵母菌水凝胶具有较好的导电性,其电导率为0.015 S/m,且具有较好的网络多孔结构,能很好的检测心电和肌电信号。
实施例 5
将0.235 g PCA溶解在9 mL 100℃去离子水中煮沸20 min,冷却至50℃,加入20wt%明胶于50℃水浴中搅拌30 min,然后加入0.15 g的葡萄糖,继续搅拌溶解得到Gel-PCA和葡萄糖的混合溶液,降温至30℃。称取0.45 g 酵母粉溶解于1 mL 30℃去离子水中。然后将酵母菌液和上述Gel-PCA和葡萄糖的混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10 min即得到Gel-PCA-酵母菌水凝胶。最后将其浸泡在20 wt%硫酸铵和甘油的混合溶液中(1:2,1:1,2:1(v/v))12 h后,进行检测。如图6和图7所示,得到的Gel-PCA-酵母菌-硫酸铵-甘油水凝胶具有较好的保水性和流变性能,浸泡硫酸铵和甘油比例为1:1和1:2时,在室温下放置三天后其质量几乎不变,表明其失水较少。流变检测结果显示浸泡硫酸铵和甘油比例为1:1时其储能模量从100 Pa增加至1000 Pa,高出未浸泡组2个数量级,表明浸泡后的水凝胶强度高于未浸泡组。
以上所述仅为本发明的较佳实施例,凡依本发明申请专利范围所做的均等变化与修饰,皆应属本发明的涵盖范围。
Claims (7)
1.一种利用酵母菌发酵制备多功能水凝胶的方法,其特征在于,包括如下步骤:
(1)将酵母菌在30℃的热水中活化得到酵母菌液;
(2)称取平板计数琼脂PCA溶解在100℃去离子水中煮沸20 min,冷却至50℃,得PCA溶液;向PCA溶液中加入明胶Gel于50℃水浴中搅拌30 min,然后加入葡萄糖,继续搅拌溶解得到Gel-PCA-葡萄糖的混合溶液,降温至30℃;
(3)将步骤(1)的酵母菌液和步骤(2)的Gel-PCA-葡萄糖的混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10 min即得到Gel-PCA-酵母菌多功能水凝胶;
步骤(1)中所述酵母菌液浓度为0.2 g/mL-0.45 g/mL;
步骤(2)中所述PCA溶液的浓度为0.0235 g/mL ;明胶的添加量为5 wt%-35 wt%;所述的葡萄糖的添加量为0.01-0.06 g/mL。
2.根据权利要求1所述的一种利用酵母菌发酵制备多功能水凝的方法,其特征在于:步骤(2)中向PCA溶液中加入浓度为1-4 mg/mL的还原氧化石墨烯PrGO;得到Gel-PrGO-PCA-葡萄糖混合溶液;将步骤(1)的酵母菌液和Gel-PrGO-PCA-葡萄糖混合溶液搅拌混匀后倒入模具中,并置于30℃水浴中发酵30 min,然后将其放在4℃下10 min即得到Gel-PrGO-PCA-酵母菌多功能水凝胶。
3.根据权利要求2所述的一种利用酵母菌发酵制备多功能水凝的方法,其特征在于:所述还原氧化石墨烯的制备方法,包括以下步骤:
(1)氧化石墨烯的制备:称取1.2 g石墨,加入50 mL 浓硫酸,搅拌均匀后,放入冰浴中,搅拌状态下加入1.5 g硝酸钠,并缓慢加入6 g高锰酸钾,于35℃下搅拌过夜,缓慢加入去离子水100 mL,控制温度为90℃,反应1 h,将30vol%的过氧化氢稀释5倍后缓慢加入上述溶液中,直到不产生气泡,停止添加,继续反应3 h,冷却至室温,水洗至中性后超声分散20 min,冻干后得到氧化石墨烯粉末;
(2)称取20 mg GO粉末,加入2.5 mL去离子水,超声直至完全溶解,得到GO分散液;称取50 mg 盐酸多巴胺粉末溶解在2.5 mL 10 mM pH=8.5 Tris-HCl溶液中,得盐酸多巴胺分散液;然后将盐酸多巴胺分散液加入到 GO分散液中,冰浴下超声分散2 h后,在60℃水浴下搅拌12 h,得到还原氧化石墨烯PrGO溶液。
4.根据权利要求1所述的一种利用酵母菌发酵制备多功能水凝的方法,其特征在于:步骤(3)中所得Gel-PCA-酵母菌水凝胶放在-80℃冷冻20 min后切成任意形状,浸泡在盐溶液和甘油的混合溶液中12 h。
5.根据权利要求4所述的一种利用酵母菌发酵制备多功能水凝的方法,其特征在于:所述盐溶液为硫酸铵或柠檬酸钠溶液;所述的盐溶液浓度为10 wt%-30 wt%;所述盐溶液与甘油的体积比为2:1、1:1或1:2。
6.如权利要求1-5任一项所述方法制备所得的多功能水凝胶。
7.如权利要求6所述多功能水凝胶的应用,其特征在于:多功能水凝胶作为导电材料用于生物传感器中,或用于制备药物载体、抗菌伤口敷料中。
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111038363.0A CN113736101B (zh) | 2021-09-06 | 2021-09-06 | 一种利用酵母菌发酵制备多功能水凝胶的方法 |
PCT/CN2022/113331 WO2023030023A1 (zh) | 2021-09-06 | 2022-08-18 | 一种利用酵母菌发酵制备多功能水凝胶的方法 |
US18/034,062 US20230365760A1 (en) | 2021-09-06 | 2022-08-18 | Method for preparing multifunctional hydrogel by yeast fermentation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111038363.0A CN113736101B (zh) | 2021-09-06 | 2021-09-06 | 一种利用酵母菌发酵制备多功能水凝胶的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113736101A CN113736101A (zh) | 2021-12-03 |
CN113736101B true CN113736101B (zh) | 2022-12-23 |
Family
ID=78736029
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111038363.0A Active CN113736101B (zh) | 2021-09-06 | 2021-09-06 | 一种利用酵母菌发酵制备多功能水凝胶的方法 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20230365760A1 (zh) |
CN (1) | CN113736101B (zh) |
WO (1) | WO2023030023A1 (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113736101B (zh) * | 2021-09-06 | 2022-12-23 | 福州大学 | 一种利用酵母菌发酵制备多功能水凝胶的方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB201020193D0 (en) * | 2010-11-29 | 2011-01-12 | Biotec Pharmacon Asa | Glucan compositions |
CN102702559B (zh) * | 2012-07-04 | 2013-09-11 | 天津工业大学 | 一种微生物发酵超多孔水凝胶及其制备方法和应用 |
CN105709701B (zh) * | 2016-01-08 | 2018-10-16 | 中南大学 | 一种负载纳米粒子的石墨烯/菌丝水凝胶及其制备方法和应用 |
CN110776652B (zh) * | 2019-10-23 | 2022-07-26 | 重庆医科大学 | 石墨烯基导电水凝胶和其制备方法及在柔性可穿戴式传感器上的应用 |
CN111154037B (zh) * | 2019-12-27 | 2021-04-30 | 浙江大学 | 多功能海藻酸钠-P(SBMA-co-AAm)离子导电水凝胶及其制备方法 |
CN113736101B (zh) * | 2021-09-06 | 2022-12-23 | 福州大学 | 一种利用酵母菌发酵制备多功能水凝胶的方法 |
-
2021
- 2021-09-06 CN CN202111038363.0A patent/CN113736101B/zh active Active
-
2022
- 2022-08-18 US US18/034,062 patent/US20230365760A1/en active Pending
- 2022-08-18 WO PCT/CN2022/113331 patent/WO2023030023A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
US20230365760A1 (en) | 2023-11-16 |
WO2023030023A1 (zh) | 2023-03-09 |
CN113736101A (zh) | 2021-12-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107602884B (zh) | 一种丝素/壳聚糖复合智能水凝胶及其制备方法 | |
CN113637183A (zh) | 改性石墨烯负载纳米银/聚乙烯醇抗菌水凝胶及其制备方法 | |
CN111944167B (zh) | 一种导电水凝胶及其制备方法和应用 | |
CN113736101B (zh) | 一种利用酵母菌发酵制备多功能水凝胶的方法 | |
CN112159535A (zh) | 部分还原氧化石墨烯-纳米纤维素晶-聚乙烯醇复合导电水凝胶及其制备方法和应用 | |
CN107345840A (zh) | 一种基于载银纳米纤维的柔性力敏传感器及其制备方法 | |
CN114230812A (zh) | 一种功能性水凝胶及其制备方法和应用 | |
CN103757917B (zh) | 一种具有电活性的壳聚糖基复合材料的制备方法 | |
CN108659237B (zh) | 一种导电性能随温度调谐的纳米纤维复合水凝胶及其制备方法和应用 | |
CN110563968A (zh) | 一种高强高拉伸的离子导电水凝胶的制备方法 | |
CN114539695A (zh) | 一种仿肌肉纤维高韧性抗菌促愈合水凝胶及其制法和应用 | |
CN111493863B (zh) | 透气抗菌电极片及其制备方法 | |
CN105797197B (zh) | 一种皮肤敷料及其制备方法 | |
CN110699855B (zh) | 一种导电壳聚糖/角蛋白纳米纤维膜的制备方法 | |
CN112430394B (zh) | 一种导电增强型聚吡咯/石墨烯/明胶复合柔性电极材料及其制备方法 | |
CN107412882B (zh) | 一种贴附柔性人工皮肤感受器的制备方法 | |
CN114736332B (zh) | 一种酶促生物盐凝胶的制备方法和应用 | |
CN107412880B (zh) | 一种柔性透明人工皮肤感受器的制备方法 | |
WO2019047043A1 (zh) | 一种丝素/壳聚糖复合智能水凝胶及其制备方法 | |
CN114674885A (zh) | 一种柔性可穿戴式汗液葡萄糖电化学传感器的制备方法 | |
CN114409926A (zh) | 一种自愈合抗冻导电丝素水凝胶及其制备方法 | |
CN114522275A (zh) | 一种定向导电纤维的三维多孔复合支架及其制备方法 | |
CN113896942B (zh) | 一种透气性表皮电极及其制备方法 | |
CN108951129A (zh) | 一种医疗卫生用透气抗菌整理粘胶非织造布的方法 | |
CN114835943B (zh) | 一种抗冻导电可拉伸硫辛酸有机凝胶的制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |