CN113678688A - Flammulina velutipes culture method based on liquid strain culture - Google Patents

Flammulina velutipes culture method based on liquid strain culture Download PDF

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Publication number
CN113678688A
CN113678688A CN202110885218.XA CN202110885218A CN113678688A CN 113678688 A CN113678688 A CN 113678688A CN 202110885218 A CN202110885218 A CN 202110885218A CN 113678688 A CN113678688 A CN 113678688A
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production
mushroom
culture
bottle
temperature
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朱志林
张莉
厉芳
朱彬峰
张俊保
郑敬慧
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Jiangsu Kang Sheng Agriculture Development Co ltd
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Jiangsu Kang Sheng Agriculture Development Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/40Cultivation of spawn
    • GPHYSICS
    • G05CONTROLLING; REGULATING
    • G05DSYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
    • G05D27/00Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00
    • G05D27/02Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00 characterised by the use of electric means

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Automation & Control Theory (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a golden mushroom cultivation method based on liquid strain cultivation, in particular to the technical field of agriculture, the golden mushroom production process flow of the invention takes a central intelligent management center as a master control room for production, and comprises an automatic pipeline system and an environment intelligent control system, thereby completing the intelligent production technology of golden mushrooms, the technology not only realizes the anti-season production and the whole-year production of the golden mushrooms, but also improves the product quality and the yield, the golden mushrooms produced in the unconventional production season have better quality and larger market demand than the golden mushrooms refrigerated by a refrigeration house, the exploration and the implementation of the intelligent production technology of the golden mushrooms can lead the production of the golden mushrooms to overcome the limitation of climatic environment, realize the annual production, increase the income of farmers, promote the industrial advantages, and realize the standardized production and the branded sale, therefore, the method has more market benefits and wider development prospect.

Description

Flammulina velutipes culture method based on liquid strain culture
Technical Field
The invention relates to the technical field of agriculture, in particular to a needle mushroom culture method based on liquid strain culture.
Background
The golden mushroom is delicious in taste and high in nutritional value, is a nutritional food which is high in protein, low in heat and rich in polysaccharide, has high nutritional value and high medicinal value, but the production of the fresh golden mushroom is seasonal, and the fresh golden mushroom is often overstocked, rotted and deteriorated due to limited processing in the high-yield season of the golden mushroom due to short storage time of the fresh golden mushroom, so that the commodity value is lost, the economic loss is caused, the predicament that raw materials are not processed is generated in the low-yield season of the golden mushroom, and the shortage of convenient food of the golden mushroom in the market is caused, so that the traditional production mode needs to be changed to realize the annual production of the golden mushroom, the prior art does not have the environmental conditions which can effectively control temperature, humidity, gas, light and the like to meet the life habit of the golden mushroom, the golden mushroom cannot be put on the market in a annual balance manner, and the economic benefit of a producer is lower, therefore, it is important to research a method for culturing flammulina velutipes based on liquid spawn culture to solve the above problems.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a needle mushroom culture method based on liquid strain culture, and the technical problems to be solved by the invention are as follows: the production of fresh flammulina velutipes is seasonal, the fresh flammulina velutipes is usually overstocked, rotted and deteriorated due to limited processing in the high-yield mushroom season so as to lose commodity value and cause economic loss, and the predicament that raw materials are not processed appears in the low-yield mushroom season so as to cause the shortage of convenient flammulina velutipes foods in the market.
In order to achieve the purpose, the invention provides the following technical scheme: a needle mushroom cultivation method based on liquid strain cultivation comprises a central intelligent management center, wherein the output end of the central intelligent management center is electrically connected with the input ends of an automatic assembly line system and an environment intelligent control system respectively, and the automatic assembly line system specifically comprises the following operation steps:
s1, liquid strain production:
the method comprises the steps of selecting cottonseed hulls, corncobs and sawdust as cultivation materials, industrially producing a liquid strain formula by taking glucose, zinc sulfate, soybean meal, beef extract, monopotassium phosphate and water as main components, obtaining a culture material base material, and performing composting fermentation on the culture material base material.
S2, stirring materials and bottling:
uniformly mixing the compost base material obtained by composting fermentation in a large-scale stirrer, and then bottling by a full-automatic bottling machine.
S3, sterilization:
the sterilization time is determined by the number and season of the culture bottles in the sterilization cabinet and the condition of the culture medium, and is controlled according to a program input in advance.
S4, cooling:
after the sterilization time is up, the pressure is reduced to normal pressure, the door can be opened when the temperature is reduced to below 95 ℃ during normal pressure sterilization, then the frame is transferred to a cooling chamber, and an air conditioner is started to reduce the temperature of the material to l6-18 ℃ so as to facilitate inoculation.
S5, inoculation:
inoculating is carried out by an automatic inoculating machine, inoculating is carried out according to the principle that the inoculating amount of each bottle is about 10g, the bottle basically covers the surface of the whole culture material, generally, 45-50 bottles are inoculated in 850mL seed bottles, and meanwhile, the environment of a mushroom house is monitored by an environment intelligent control system.
S6, culturing:
the temperature of the culture room is 14-l6 ℃, the humidity is kept at 70-80%, the concentration of carbon dioxide is controlled to be less than 3000 multiplied by 10 < -6 >, and the culture room can be filled after 25-31 days.
S7, mycelium stimulation:
scratching the mycelia after the mycelia are full, and finishing by using a mycelium scratching machine, wherein the depth is generally the initial position of the bottle shoulder.
S8, fruiting:
the fruiting comprises the following 4 steps of bud forcing, buffering, inhibiting and breeding:
a. bud forcing: keeping the temperature at 15-16 deg.C and humidity at 90-95%, controlling carbon dioxide concentration, and scattering light for 1 hr every day for 8-10 days to obtain buds.
b. Buffering: in order to prevent the fruiting body with weak resistance from withering and enhance the resistance, when the mushroom buds grow to 13-15cm, the mushroom buds need to be transferred to a buffer chamber for buffer treatment, and the temperature and humidity conditions are between the bud forcing chamber and the inhibition chamber.
c. Inhibition: the temperature of the inhibition chamber is 3-5 ℃, the humidity is 70% -80%, the purpose is to inhibit the size and the growth of the fast sporocarp obviously, thereby achieving the purpose of aligning and inhibiting the sporocarp by 2 forms of light inhibition and air blowing inhibition.
d. And (3) breeding: the young mushroom can be transferred to a growing room after being inhibited, the temperature of the growing room is 7-9 ℃, the humidity is 75% -80%, and the concentration of carbon dioxide is controlled below 3000 multiplied by 10 < -6 >.
S9, harvesting and packaging:
when the mushrooms grow out of the bottle mouth by 13-14cm, the mushrooms can be harvested, the operation needs to be carried out in a clean and low-temperature room, the yield of each bottle adopting corncobs as raw materials can reach 160-180g, and the harvested needle mushrooms are packaged by combining nano modified atmosphere and then placed into a refrigeration house for storage.
S10, digging the bottle:
after the needle mushrooms are harvested, the waste materials are removed by a bottle digging machine, and then the inside and the outside of the bottle are cleaned and dried, so that the next cycle can be carried out.
S11, testing and treating leftovers:
and (3) carrying out quality inspection on the obtained needle mushroom products, selling the needle mushroom products out of the factory after the quality is confirmed, and returning leftovers generated in the production process to the field after harmless treatment.
As a further scheme of the invention: note that in S2: the stirring time is not suitable to be overlong in summer, the materials are stirred and then sterilized in time, the water content is controlled to be about 63-65%, the bottling requirement is that the weight is consistent, the materials are tightened and loosened, and good ventilation and uniform spawn running are ensured.
As a further scheme of the invention: the mass ratio of the cotton seed shells, the corn cobs and the wood chips in the S1 is 3:6: 2.
As a further scheme of the invention: the environment intelligent control system comprises a mushroom house 1 and a mushroom house n, wherein the mushroom house 1 comprises a temperature sensor, a humidity sensor, an illumination sensor and a carbon dioxide sensor, and output ends of the temperature sensor, the humidity sensor, the illumination sensor and the carbon dioxide sensor are electrically connected with an input end of the same controller through A/D conversion.
As a further scheme of the invention: the output end of the controller is electrically connected with the output end of the RS485 transceiver 1, the RS485 transceiver 1 and a plurality of RS485 transceivers n are electrically connected with the same RS485/RS232 conversion module in a two-way mode, and the RS485/RS232 conversion module is electrically connected with the central control computer in a two-way mode.
As a further scheme of the invention: and the output end of the controller is respectively and electrically connected with the input ends of the crystal rolling screen, the air conditioner, the humidifier and the lighting.
As a further scheme of the invention: the mycelium in S7 has 2 functions: firstly, hypha is mechanically stimulated, which is beneficial to fruiting, and secondly, the surface of the compost is scratched to make the fruiting regular.
As a further scheme of the invention: the parameters of scattered light in the bud forcing process in the S8 are 50-100lx, and the concentration of carbon dioxide in the bud forcing process in the S2 is controlled below 3000 x 10 < -6 >.
The invention has the beneficial effects that:
1. the needle mushroom production process flow takes a central intelligent management center as a master control room for production, and comprises an automatic pipeline system and an environment intelligent control system, so that an intelligent needle mushroom production technology is completed, the technology not only realizes anti-season production and annual production of needle mushrooms, but also improves the product quality and yield, the produced needle mushrooms in unconventional production seasons have better quality and larger market demand than the needle mushrooms refrigerated in a cold storage, and through exploration and implementation of the needle mushroom intelligent production technology, the production of the needle mushrooms can overcome the limitation of climate environment, annual production is realized, the income of farmers is increased, the industrial advantages are improved, and meanwhile, standardized production and branded sale are realized, so that the needle mushrooms have more market benefits and have wider development prospects;
2. according to the invention, an automatic assembly line system is arranged, and the automatic assembly line system is an automatic production process flow consisting of a material mixing and bottling integrated machine, an autoclave boiler, an automatic inoculation machine, an automatic fungus scratching machine, an automatic conveying belt, an automatic packaging machine, an automatic bottle digging machine and the like, collected production information is transmitted to a central control room through video collection, and the production condition of a mushroom house is known through display after processing;
3. the invention discloses a method for controlling edible mushroom culture medium, which comprises the steps of setting an environment intelligent control system, wherein the environment intelligent control system comprises an automatic temperature control system, lighting equipment and the like, is responsible for environmental regulation and control and microbial control of the whole growth cycle of golden mushroom, predicting the threshold value of pollution outbreak through researching the quantity and population change of microorganisms of each node in the factory production of edible mushroom, forming a factory production pollution early warning system, analyzing the correlation among pollution sources, pollution quantity and yield and quality in the production process of golden mushroom strains, finding out the main pollution source which most easily influences the production of edible mushrooms, carrying out pollution control and regional treatment work in advance, developing high-efficiency sterilization processes and equipment by self, improving the anti-pollution equipment technology and performance of key processes such as inoculation, monitoring and the like, continuously monitoring the pollution degree of environment dead corners such as an air filtration system, a triangular flask, an inoculation chamber, a culture chamber and the like, the method comprises the steps of establishing replacement indexes and threshold parameters, establishing control measures which are identical with microbial propagation accumulation all the year round, combining an inoculation technology for shortening the vegetative growth period with pollution reduction, space utilization rate improvement and quality improvement, enabling the stability of the production process to be uniform with the stability of product yield and quality, establishing an intelligent control system and software, obtaining a modern factory low-pollution and high-stability production and control technology for edible fungi, and designing a comprehensive utilization scheme and a system for sterilizing waste heat and cooling water resources in the pollution control process to achieve the maximization of factory production resource utilization.
Drawings
FIG. 1 is a schematic view of the process flow of the production of Flammulina velutipes of the present invention;
FIG. 2 is a schematic structural diagram of an environment intelligent control system according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A needle mushroom cultivation method based on liquid strain cultivation comprises a central intelligent management center, wherein the output end of the central intelligent management center is respectively and electrically connected with the input end of an automatic pipeline system and the input end of an environment intelligent control system, the needle mushroom cultivation method is characterized in that an automatic pipeline system is arranged, the automatic pipeline system is an automatic production process flow which is composed of a material mixing and bottling all-in-one machine, a high-pressure sterilization boiler, an automatic inoculation machine, an automatic fungus scratching machine, an automatic conveying belt, an automatic packing machine, an automatic bottle digging machine and the like, collected production information is transmitted to a central control room through video collection, the production condition of a mushroom house is known through display after processing, and the automatic pipeline system specifically comprises the following operation steps:
s1, liquid strain production:
the method comprises the steps of selecting cottonseed hulls, corncobs and sawdust as cultivation materials, industrially producing a liquid strain formula by taking glucose, zinc sulfate, soybean meal, beef extract, monopotassium phosphate and water as main components to obtain a culture material base material, stacking and fermenting the culture material base material, wherein the advantages of flammulina velutipes liquid strains are obvious compared with those of paper pulp strains, the strain production period is reduced by more than 1 month compared with the use of the paper pulp strains, during the flammulina velutipes strain liquid culture process, strains suitable for carrying out flammulina velutipes strain liquid culture can be selected according to various factors such as climate, environment, geography, soil and the like in Xuzhou regions, and the optimal culture medium is determined by taking the strain growth period, the edible fungus culture period, the growth rate and the pollution rate as indexes.
S2, stirring materials and bottling:
uniformly mixing the compost base material obtained by composting fermentation in a large-scale stirrer, and then bottling by a full-automatic bottling machine.
S3, sterilization:
the sterilization time is determined by the number and season of the culture bottles in the sterilization cabinet and the condition of the culture medium, and is controlled according to a program input in advance.
S4, cooling:
after the sterilization time is up, the pressure is reduced to normal pressure, the door can be opened when the temperature is reduced to below 95 ℃ during normal pressure sterilization, then the frame is transferred to a cooling chamber, and an air conditioner is started to reduce the temperature of the material to l6-18 ℃ so as to facilitate inoculation.
S5, inoculation:
inoculating is carried out by an automatic inoculating machine, inoculating is carried out according to the principle that the inoculating amount of each bottle is about 10g, the bottle basically covers the surface of the whole culture material, generally, 45-50 bottles are inoculated in 850mL seed bottles, and meanwhile, the environment of a mushroom house is monitored by an environment intelligent control system.
S6, culturing:
the temperature of the culture room is 14-l6 ℃, the humidity is kept at 70-80%, the concentration of carbon dioxide is controlled to be less than 3000 multiplied by 10 < -6 >, and the culture room can be filled after 25-31 days.
S7, mycelium stimulation:
scratching the mycelia after the mycelia are full, and finishing by using a mycelium scratching machine, wherein the depth is generally the initial position of the bottle shoulder.
S8, fruiting:
the fruiting comprises the steps of bud forcing, buffering, inhibiting and growing 4:
a. bud forcing: keeping the temperature at 15-16 deg.C and humidity at 90-95%, controlling carbon dioxide concentration, and scattering light for 1 hr every day for 8-10 days to obtain buds.
b. Buffering: in order to prevent the fruiting body with weak resistance from withering and enhance the resistance, when the mushroom buds grow to 13-15cm, the mushroom buds need to be transferred to a buffer chamber for buffer treatment, and the temperature and humidity conditions are between the bud forcing chamber and the inhibition chamber.
c. Inhibition: the temperature of the inhibition chamber is 3-5 ℃, the humidity is 70% -80%, the purpose is to inhibit the size and the growth of the fast sporocarp obviously, thereby achieving the purpose of aligning and inhibiting the sporocarp by 2 forms of light inhibition and air blowing inhibition.
d. And (3) breeding: the young mushroom can be transferred to a growing room after being inhibited, the temperature of the growing room is 7-9 ℃, the humidity is 75% -80%, and the concentration of carbon dioxide is controlled below 3000 multiplied by 10 < -6 >.
S9, harvesting and packaging:
when the mushrooms grow out of the bottle mouth by 13-14cm, the mushrooms can be harvested, the operation needs to be carried out in a clean and low-temperature room, the yield of each bottle adopting corncobs as raw materials can reach 160-180g, the harvested quality of the flammulina velutipes is packaged by combining nano modified atmosphere and then placed into a refrigeration house for storage, and the shelf life of the flammulina velutipes can be prolonged by the method.
S10, digging the bottle:
after the needle mushrooms are harvested, the waste materials are removed by a bottle digging machine, and then the inside and the outside of the bottle are cleaned and dried, so that the next cycle can be carried out.
S11, testing and treating leftovers:
and (3) carrying out quality inspection on the obtained needle mushroom products, selling the needle mushroom products out of the factory after the quality is confirmed, and returning leftovers generated in the production process to the field after harmless treatment.
Note that in S2: the stirring time is not suitable to be overlong in summer, the materials are stirred and then sterilized in time, the water content is controlled to be about 63-65%, the bottling requirement is that the weight is consistent, the materials are tightened and loosened, and good ventilation and uniform spawn running are ensured.
In the S1, the mass ratio of the cotton seed shells to the corn cobs to the wood chips is 3:6: 2.
The environment intelligent control system comprises a mushroom house 1-mushroom house n, the mushroom house 1 comprises a temperature sensor, a humidity sensor, an illumination sensor and a carbon dioxide sensor, and output ends of the temperature sensor, the humidity sensor, the illumination sensor and the carbon dioxide sensor are electrically connected with an input end of the same controller through A/D conversion.
The output end of the controller is electrically connected with the output end of the RS485 transceiver 1, the RS485 transceiver 1 and a plurality of RS485 transceivers n are electrically connected with the same RS485/RS232 conversion module in a bidirectional mode, and the RS485/RS232 conversion module is electrically connected with the central control computer in a bidirectional mode.
The output end of the controller is respectively and electrically connected with the input ends of the crystal rolling screen, the air conditioner, the humidifier and the lighting, the crystal rolling screen, the air conditioner, the humidifier and the lighting are arranged to be responsible for environment regulation and control and microorganism control of the whole growth period of the flammulina velutipes, the threshold value of pollution outbreak is predicted through the change research on the quantity and population of microorganisms of each node of the flammulina velutipes edible fungi in an industrial mode, an industrial production pollution early warning system is formed, the correlation among pollution sources, pollution quantity and yield and quality in the flammulina velutipes strain production process is analyzed, the main pollution sources which are most likely to influence the production of the edible fungi are found out, and pollution control and area processing work are carried out in advance.
In S7, the mycelium has 2 effects: firstly, hypha is mechanically stimulated, which is beneficial to fruiting, and secondly, the surface of the compost is scratched to make the fruiting regular.
The parameters of scattered light in the bud forcing process in the S8 are 50-100lx, and the carbon dioxide concentration in the bud forcing process in the S2 is controlled below 3000 x 10 < -6 >.
Tests show that in the field of industrial production of edible fungi, the flammulina velutipes liquid strain has obvious advantages compared with a paper pulp strain, the strain production period is shortened by more than one month compared with the use of the paper pulp strain, the period for growing the strain and growing the mushroom in a culture bottle is shortened by about 4 days compared with the use of the paper pulp strain, the whole period is shortened by about 40 days, the pollution rate is reduced from about 0.45% to about 0.16% after the paper pulp strain is changed into the liquid strain, and the pollution rate is maintained at a stable level.
The points to be finally explained are: although the present invention has been described in detail with reference to the general description and the specific embodiments, on the basis of the present invention, the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims (8)

1. A needle mushroom cultivation method based on liquid strain cultivation comprises a central intelligent management center and is characterized in that an output end of the central intelligent management center is electrically connected with an input end of an automatic pipeline system and an input end of an environment intelligent control system respectively, and the automatic pipeline system specifically comprises the following operation steps:
s1, liquid strain production:
selecting cottonseed hull, corncob and sawdust as cultivation materials, and industrially producing a liquid strain formula by using glucose, zinc sulfate, soybean meal, beef extract, monopotassium phosphate and water as main components to obtain a culture material base material, and stacking and fermenting the culture material base material;
s2, stirring materials and bottling:
uniformly mixing the compost base material obtained by composting fermentation in a large-scale stirrer, and then bottling by a full-automatic bottling machine;
s3, sterilization:
the sterilization time is determined according to the number and season of the culture bottles in the sterilization cabinet and the condition of the culture medium, and is controlled according to a program input in advance;
s4, cooling:
after the sterilization time is up, reducing the pressure to normal pressure, opening the door when the temperature is reduced to below 95 ℃ during normal-pressure sterilization, then transferring the frame to a cooling chamber, and starting an air conditioner to reduce the temperature of the material to l6-18 ℃ so as to facilitate inoculation;
s5, inoculation:
inoculating by an automatic inoculating machine, wherein inoculating is carried out according to the principle that each bottle of inoculating amount is about 10g, and the bottle of inoculating amount basically covers the surface of the whole culture material, generally, 45-50 bottles of seeds are inoculated in 850mL seed bottles, and meanwhile, the environment of a mushroom house is monitored by an environment intelligent control system;
s6, culturing:
the temperature of the culture room is 14-l6 ℃, the humidity is kept at 70-80%, the concentration of carbon dioxide is controlled to be less than 3000 multiplied by 10 < -6 >, and the culture room can be filled after 25-31 days;
s7, mycelium stimulation:
scratching the mycelia after the mycelia are full, and finishing by using a mycelium scratching machine, wherein the depth is generally the initial position of the bottle shoulder;
s8, fruiting:
the fruiting comprises the following 4 steps of bud forcing, buffering, inhibiting and breeding:
a. bud forcing: keeping the temperature at 15-16 deg.C and humidity at 90-95%, controlling carbon dioxide concentration, and providing 1h of scattered light every day for 8-10 days to obtain buds;
b. buffering: in order to prevent the fruiting body with weak resistance from withering and enhance the resistance, when the mushroom buds grow to 13-15cm, the mushroom buds need to be transferred to a buffer chamber for buffer treatment, and the temperature and humidity conditions are between a bud forcing chamber and a suppression chamber;
c. inhibition: the temperature of the inhibition chamber is 3-5 ℃, the humidity is 70% -80%, the purpose is to inhibit the size and the growth of the fast sporocarp obviously, so that the method for achieving alignment and inhibition mainly comprises 2 forms of illumination inhibition and blowing inhibition;
d. and (3) breeding: the young mushroom can be transferred to a growing room after being inhibited, the temperature of the growing room is 7-9 ℃, the humidity is 75% -80%, and the concentration of carbon dioxide is controlled below 3000 multiplied by 10 < -6 >;
s9, harvesting and packaging:
when the mushrooms grow out of the bottle mouth by 13-14cm, the mushrooms can be harvested, the operation needs to be carried out in a clean and low-temperature room, the yield of each bottle adopting corncobs as raw materials can reach 160-180g, and the harvested needle mushrooms are packaged by combining nano modified atmosphere and then placed into a refrigeration house for storage;
s10, digging the bottle:
after the needle mushrooms are harvested, the waste materials are removed by a bottle digging machine, and then the inside and the outside of the bottle are cleaned and dried, so that the next round of circulation can be carried out;
s11, testing and treating leftovers:
and (3) carrying out quality inspection on the obtained needle mushroom products, selling the needle mushroom products out of the factory after the quality is confirmed, and returning leftovers generated in the production process to the field after harmless treatment.
2. The needle mushroom cultivation method based on liquid spawn cultivation according to claim 1, characterized in that: note that in S2: the stirring time is not suitable to be overlong in summer, the materials are stirred and then sterilized in time, the water content is controlled to be about 63-65%, the bottling requirement is that the weight is consistent, the materials are tightened and loosened, and good ventilation and uniform spawn running are ensured.
3. The needle mushroom cultivation method based on liquid spawn cultivation according to claim 1, characterized in that: the mass ratio of the cotton seed shells, the corn cobs and the wood chips in the S1 is 3:6: 2.
4. The needle mushroom cultivation method based on liquid spawn cultivation according to claim 1, characterized in that: the environment intelligent control system comprises a mushroom house 1 and a mushroom house n, wherein the mushroom house 1 comprises a temperature sensor, a humidity sensor, an illumination sensor and a carbon dioxide sensor, and output ends of the temperature sensor, the humidity sensor, the illumination sensor and the carbon dioxide sensor are electrically connected with an input end of the same controller through A/D conversion.
5. The method for cultivating flammulina velutipes based on liquid spawn cultivation according to claim 4, wherein the method comprises the following steps: the output end of the controller is electrically connected with the output end of the RS485 transceiver 1, the RS485 transceiver 1 and a plurality of RS485 transceivers n are electrically connected with the same RS485/RS232 conversion module in a two-way mode, and the RS485/RS232 conversion module is electrically connected with the central control computer in a two-way mode.
6. The method for cultivating flammulina velutipes based on liquid spawn cultivation according to claim 4, wherein the method comprises the following steps: and the output end of the controller is respectively and electrically connected with the input ends of the crystal rolling screen, the air conditioner, the humidifier and the lighting.
7. The needle mushroom cultivation method based on liquid spawn cultivation according to claim 1, characterized in that: the mycelium in S7 has 2 functions: firstly, hypha is mechanically stimulated, which is beneficial to fruiting, and secondly, the surface of the compost is scratched to make the fruiting regular.
8. The needle mushroom cultivation method based on liquid spawn cultivation according to claim 1, characterized in that: the parameters of scattered light in the bud forcing process in the S8 are 50-100lx, and the concentration of carbon dioxide in the bud forcing process in the S2 is controlled below 3000 x 10 < -6 >.
CN202110885218.XA 2021-08-03 2021-08-03 Flammulina velutipes culture method based on liquid strain culture Pending CN113678688A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115250827A (en) * 2022-08-24 2022-11-01 江苏菇本堂生物科技股份有限公司 Method for comprehensively preventing and treating velvet antler mushroom industrial production pollution

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