CN113677808A - 用于使用cfDNA诊断癌症的方法 - Google Patents

用于使用cfDNA诊断癌症的方法 Download PDF

Info

Publication number
CN113677808A
CN113677808A CN202080027434.4A CN202080027434A CN113677808A CN 113677808 A CN113677808 A CN 113677808A CN 202080027434 A CN202080027434 A CN 202080027434A CN 113677808 A CN113677808 A CN 113677808A
Authority
CN
China
Prior art keywords
cfdna
cancer
diagnosing
probe
conditions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202080027434.4A
Other languages
English (en)
Chinese (zh)
Inventor
曺荣南
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiannuo Psychology Co ltd
Original Assignee
Jiannuo Psychology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiannuo Psychology Co ltd filed Critical Jiannuo Psychology Co ltd
Publication of CN113677808A publication Critical patent/CN113677808A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/101Temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/125Nucleic acid detection characterized by the use of physical, structural and functional properties the label being enzymatic, i.e. proteins, and non proteins, such as nucleic acid with enzymatic activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/155Particles of a defined size, e.g. nanoparticles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/118Prognosis of disease development
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN202080027434.4A 2019-04-05 2020-04-03 用于使用cfDNA诊断癌症的方法 Pending CN113677808A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR20190039974 2019-04-05
KR10-2019-0039974 2019-04-05
KR20190157368 2019-11-29
KR10-2019-0157368 2019-11-29
PCT/KR2020/004602 WO2020204674A2 (ko) 2019-04-05 2020-04-03 Cfdna를 이용한 암 진단방법

Publications (1)

Publication Number Publication Date
CN113677808A true CN113677808A (zh) 2021-11-19

Family

ID=72666861

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080027434.4A Pending CN113677808A (zh) 2019-04-05 2020-04-03 用于使用cfDNA诊断癌症的方法

Country Status (7)

Country Link
US (1) US20220170108A1 (enExample)
EP (1) EP3950962A4 (enExample)
JP (2) JP7782835B2 (enExample)
KR (10) KR102526906B1 (enExample)
CN (1) CN113677808A (enExample)
TW (1) TW202104598A (enExample)
WO (1) WO2020204674A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116219012A (zh) * 2022-12-15 2023-06-06 华中科技大学同济医学院附属同济医院 基于血浆cfDNA片段分布特征预测宫颈癌新辅助化疗效果或复发高危分类的系统及方法
CN118497344A (zh) * 2024-05-11 2024-08-16 青岛可立生物医药有限公司 用于结直肠癌诊断及分型的生物标志物及筛选方法

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2627085B (en) 2019-11-06 2024-11-13 Univ Leland Stanford Junior Methods and systems for analysing nucleic acid molecules
WO2022203093A1 (ko) * 2021-03-22 2022-09-29 이원다이애그노믹스(주) 암 발생여부를 진단 또는 예측하는 방법
US11783912B2 (en) 2021-05-05 2023-10-10 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for analyzing nucleic acid molecules
CN113718031B (zh) * 2021-08-17 2022-05-20 浙江大学医学院附属妇产科医院 一种卵巢癌早期诊断组合物的建立
KR20250030880A (ko) 2023-08-25 2025-03-05 경상국립대학교병원 폐암 치료 예후 예측을 위한 정보제공방법 및 폐암 치료 예후 예측용 조성물
KR20250074714A (ko) * 2023-11-15 2025-05-28 주식회사 진캐스트 Rna를 이용한 폐암 변이 유전자의 대규모 다중 검출 방법 및 이를 이용한 폐암 변이 유전자의 대규모 다중 검출 키트
WO2025127193A1 (ko) * 2023-12-13 2025-06-19 연세대학교 산학협력단 높은 sfrp4의 발현을 보이는 암의 치료방법
WO2025213150A1 (en) * 2024-04-06 2025-10-09 Precede Biosciences, Inc. Methods, kits and systems for measuring psa and psma expression and methods for treating cancer based on same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107709578A (zh) * 2015-06-25 2018-02-16 拉梅什·瓦拉巴恩尼 使用靶向特异性dna探针在生物样品中进行染色体多重分析的方法和系统
CN107980061A (zh) * 2015-06-23 2018-05-01 国立癌症中心 使用导电聚合物的游离dna捕获平台以及该平台的应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070044048A (ko) * 2004-08-11 2007-04-26 더 클리브랜드 클리닉 파운데이션 갑상선암의 검출방법
US9315802B2 (en) * 2009-12-30 2016-04-19 Quest Diagnostics Investments Incorporated RNA isolation from soluble urine fractions
EP3218523B1 (en) * 2014-11-14 2020-02-12 Liquid Genomics Inc. Use of circulating cell-free rna for diagnosis and/or monitoring cancer
WO2016145323A1 (en) * 2015-03-11 2016-09-15 The Governing Council Of The University Of Toronto An electrochemical clamp assay
MA45480A (fr) * 2015-03-12 2018-01-17 Janssen Pharmaceutica Nv Marqueurs d'arnm à base de sang total pour prédire un cancer de la prostate, et leurs procédés de détection
KR101751962B1 (ko) 2015-04-30 2017-06-30 주식회사 넥스바이오 세포-유리형 dna의 위암 진단 용도
KR20170124683A (ko) * 2016-05-02 2017-11-13 연세대학교 산학협력단 담도암 진단용 신규 바이오마커로서의 융합 유전자 및 융합 단백질
KR101925125B1 (ko) 2016-06-16 2018-12-04 울산대학교 산학협력단 Nckap1을 유효성분으로 포함하는 대장암 진단 또는 대장암 전이 예후 예측용 바이오마커 조성물
KR101818352B1 (ko) * 2016-06-30 2018-01-15 충북대학교 산학협력단 방광암의 진단 및 예후 예측용 바이오 마커 및 그의 용도
KR101980819B1 (ko) * 2016-12-23 2019-05-21 주식회사 제놉시 자성 나노 입자가 탑재된 전도성 고분자 및 양이온성 고분자를 포함하는 세포-유리(cell-free) DNA 검출 및 회수용 자성 나노 구조체
US10870891B2 (en) * 2017-01-05 2020-12-22 Biodesix, Inc. Diagnostic test system for specific, sensitive and reproducible detection of circulating nucleic acids in whole blood
KR20200003917A (ko) * 2017-05-10 2020-01-10 난토믹스, 엘엘씨 암의 검출, 예측, 및 모니터링을 위한 순환 rna(circulating rna for detection, prediction, and monitoring of cancer)
CN107164543A (zh) 2017-06-16 2017-09-15 上海长海医院 Kras 作为胰腺癌中生物标志物的用途
JP2021526029A (ja) * 2018-06-01 2021-09-30 ジェノプシー・カンパニー・リミテッドGenopsy Co., Ltd. 不安定セルフリーdnaを検出する方法およびそれを使用するデバイス

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107980061A (zh) * 2015-06-23 2018-05-01 国立癌症中心 使用导电聚合物的游离dna捕获平台以及该平台的应用
CN107709578A (zh) * 2015-06-25 2018-02-16 拉梅什·瓦拉巴恩尼 使用靶向特异性dna探针在生物样品中进行染色体多重分析的方法和系统

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JAGOTAMOY DAS等: ""An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum"", 《NATURE CHEMISTRY》, vol. 7, 1 June 2015 (2015-06-01), pages 573 - 574 *
陈晓光: "《药理学研究的新技术与新方法》", vol. 1, 31 March 2014, 中国协和医科大学出版社, pages: 188 *
陈英: "《精神疾病的分子生物学与遗传研究》", vol. 1, 31 March 2015, 科学技术文献出版社, pages: 171 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116219012A (zh) * 2022-12-15 2023-06-06 华中科技大学同济医学院附属同济医院 基于血浆cfDNA片段分布特征预测宫颈癌新辅助化疗效果或复发高危分类的系统及方法
CN118497344A (zh) * 2024-05-11 2024-08-16 青岛可立生物医药有限公司 用于结直肠癌诊断及分型的生物标志物及筛选方法

Also Published As

Publication number Publication date
KR102745889B1 (ko) 2024-12-23
KR20200117914A (ko) 2020-10-14
KR20200117907A (ko) 2020-10-14
KR20200117912A (ko) 2020-10-14
KR102745852B1 (ko) 2024-12-23
WO2020204674A2 (ko) 2020-10-08
US20220170108A1 (en) 2022-06-02
KR102372425B1 (ko) 2022-03-10
KR102745908B1 (ko) 2024-12-23
KR102526913B1 (ko) 2023-05-02
WO2020204674A3 (ko) 2020-12-17
JP2026021394A (ja) 2026-02-10
KR102526906B1 (ko) 2023-05-02
EP3950962A2 (en) 2022-02-09
KR20200117915A (ko) 2020-10-14
KR20200117909A (ko) 2020-10-14
KR102745906B1 (ko) 2024-12-23
TW202104598A (zh) 2021-02-01
KR20200117911A (ko) 2020-10-14
KR102745894B1 (ko) 2024-12-23
KR102745900B1 (ko) 2024-12-23
KR20200117913A (ko) 2020-10-14
JP2022528278A (ja) 2022-06-09
KR102526916B1 (ko) 2023-05-02
JP7782835B2 (ja) 2025-12-09
EP3950962A4 (en) 2023-01-11
KR20200117917A (ko) 2020-10-14
KR20200117916A (ko) 2020-10-14
KR20200117910A (ko) 2020-10-14

Similar Documents

Publication Publication Date Title
KR102526906B1 (ko) Cfdna를 이용한 유방암 진단방법
JP2022528278A5 (enExample)
CN115917009A (zh) 结肠直肠肿物的检测
CN107980061A (zh) 使用导电聚合物的游离dna捕获平台以及该平台的应用
JP7569110B2 (ja) 不安定セルフリーdnaを検出する方法およびそれを使用するデバイス
Oguzhan Caglayan Electrochemical aptasensors for early cancer diagnosis: a review
Mirzaeinia Colorectal cancer: Biomarker landscape
RU2779746C2 (ru) Способ детекции нестабильной внеклеточной днк и устройство с его использованием
Liu et al. Fluorescence method-based nonlinear hybridization chain reaction for highly sensitive and specific detection of HER2

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination