CN113558171A - Preparation method of antibacterial mycotoxin adsorbing nano montmorillonite feed additive - Google Patents
Preparation method of antibacterial mycotoxin adsorbing nano montmorillonite feed additive Download PDFInfo
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- CN113558171A CN113558171A CN202010347861.2A CN202010347861A CN113558171A CN 113558171 A CN113558171 A CN 113558171A CN 202010347861 A CN202010347861 A CN 202010347861A CN 113558171 A CN113558171 A CN 113558171A
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- mycotoxin
- feed additive
- livestock
- nano montmorillonite
- antibacterial
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- 229910052901 montmorillonite Inorganic materials 0.000 title claims abstract description 46
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 231100000678 Mycotoxin Toxicity 0.000 title claims abstract description 36
- 239000002636 mycotoxin Substances 0.000 title claims abstract description 36
- 239000003674 animal food additive Substances 0.000 title claims abstract description 35
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 36
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 36
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims abstract description 32
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims abstract description 30
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 28
- 239000000843 powder Substances 0.000 claims abstract description 26
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 23
- 235000019260 propionic acid Nutrition 0.000 claims abstract description 16
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims abstract description 16
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims abstract description 15
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 claims abstract description 15
- 239000001632 sodium acetate Substances 0.000 claims abstract description 15
- 235000017454 sodium diacetate Nutrition 0.000 claims abstract description 15
- 239000011787 zinc oxide Substances 0.000 claims abstract description 15
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 14
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 14
- 239000011718 vitamin C Substances 0.000 claims abstract description 14
- 239000002245 particle Substances 0.000 claims description 6
- 229920001503 Glucan Polymers 0.000 claims description 5
- 229920000057 Mannan Polymers 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 238000001179 sorption measurement Methods 0.000 abstract description 13
- 244000144972 livestock Species 0.000 abstract description 11
- 238000000034 method Methods 0.000 abstract description 11
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 244000144977 poultry Species 0.000 abstract description 7
- 230000035755 proliferation Effects 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 4
- 230000000968 intestinal effect Effects 0.000 abstract description 3
- 206010012735 Diarrhoea Diseases 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract description 2
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 229940088710 antibiotic agent Drugs 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000005342 ion exchange Methods 0.000 abstract description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 20
- 238000000855 fermentation Methods 0.000 description 20
- 230000004151 fermentation Effects 0.000 description 20
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 20
- 239000000047 product Substances 0.000 description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
- 239000007788 liquid Substances 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 230000000694 effects Effects 0.000 description 12
- 238000002156 mixing Methods 0.000 description 11
- 229920001661 Chitosan Polymers 0.000 description 10
- 239000001888 Peptone Substances 0.000 description 10
- 108010080698 Peptones Proteins 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 238000000889 atomisation Methods 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 10
- 238000012258 culturing Methods 0.000 description 10
- 238000001035 drying Methods 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 10
- 239000004310 lactic acid Substances 0.000 description 10
- 235000014655 lactic acid Nutrition 0.000 description 10
- 239000003094 microcapsule Substances 0.000 description 10
- 235000019319 peptone Nutrition 0.000 description 10
- 239000010802 sludge Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 229930195730 Aflatoxin Natural products 0.000 description 6
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 239000005409 aflatoxin Substances 0.000 description 6
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 5
- 239000007836 KH2PO4 Substances 0.000 description 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 5
- 108010009736 Protein Hydrolysates Proteins 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 229940072056 alginate Drugs 0.000 description 5
- 229920000615 alginic acid Polymers 0.000 description 5
- 235000010443 alginic acid Nutrition 0.000 description 5
- 239000001110 calcium chloride Substances 0.000 description 5
- 229910001628 calcium chloride Inorganic materials 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- LINOMUASTDIRTM-QGRHZQQGSA-N deoxynivalenol Chemical compound C([C@@]12[C@@]3(C[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 LINOMUASTDIRTM-QGRHZQQGSA-N 0.000 description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 235000013312 flour Nutrition 0.000 description 5
- 238000004108 freeze drying Methods 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 5
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 5
- 229930182817 methionine Natural products 0.000 description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 5
- 239000002244 precipitate Substances 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- MBMQEIFVQACCCH-UHFFFAOYSA-N trans-Zearalenon Natural products O=C1OC(C)CCCC(=O)CCCC=CC2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-UHFFFAOYSA-N 0.000 description 5
- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 description 5
- 229930003231 vitamin Natural products 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 230000007547 defect Effects 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
- 241000228197 Aspergillus flavus Species 0.000 description 2
- 241000193155 Clostridium botulinum Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 2
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 description 2
- 229910000323 aluminium silicate Inorganic materials 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- CQBLUJRVOKGWCF-UHFFFAOYSA-N [O].[AlH3] Chemical compound [O].[AlH3] CQBLUJRVOKGWCF-UHFFFAOYSA-N 0.000 description 1
- OBNDGIHQAIXEAO-UHFFFAOYSA-N [O].[Si] Chemical compound [O].[Si] OBNDGIHQAIXEAO-UHFFFAOYSA-N 0.000 description 1
- 229910001579 aluminosilicate mineral Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 229910052622 kaolinite Inorganic materials 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- -1 yeast cell walls Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/273—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/358—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a preparation method of an antibacterial mycotoxin adsorbing nano montmorillonite feed additive, belonging to the field of livestock and poultry feed additives, and the product is prepared from the following raw materials in percentage by weight: 40-50% of nano montmorillonite, 1-5% of zinc oxide, 4-6% of yeast cell wall, 5-8% of bacillus subtilis freeze-dried powder, 4-5% of vitamin C, 10-15% of propionic acid/propionate and 7-11% of sodium diacetate. The feed additive has strong ion exchange capacity and mycotoxin adsorption capacity, does not generate nutrient loss, can effectively prevent and treat livestock and poultry diarrhea, inhibits the proliferation of fungi, adsorbs and degrades mycotoxin, reduces the influence of mycotoxin on the growth of livestock and poultry, improves the balance of intestinal flora of livestock and poultry, improves the growth performance of livestock and poultry, and effectively reduces the use of antibiotics in the livestock and poultry breeding process.
Description
Technical Field
The invention relates to the field of feed additives, in particular to a preparation method of an antibacterial mycotoxin adsorbing nano montmorillonite feed additive.
Background
In the process of animal growth, various harmful bacteria are accompanied, some can affect the health and normal immunity of animal bodies by metabolizing nutrient substances and some can affect the health and normal immunity of animal bodies by generating toxins, so that the utilization rate and the growth performance of feed are reduced.
At present, in the feed additive market, zinc oxide, copper sulfate, peracetic acid, propionic acid, yeast cell walls, probiotics and the like are usually adopted to inhibit the proliferation of bacteria and fungi, the antibacterial effect is exerted by adding copper and zinc into the feed additive, the growth of animals can be promoted, the harmful bacteria are blocked from being planted in intestinal tracts mainly through the yeast cell walls and the metabolites of the probiotics by adding the yeast cell walls and the probiotics, the proliferation of the beneficial bacteria in the intestinal tracts can be promoted, and the balance of intestinal flora is improved.
Montmorillonite (also known as microcrystalline kaolinite or montmorillonite) is an aluminosilicate, the main component of which is octahedral montmorillonite particles, and 2:1 layered aluminosilicate minerals containing crystal water, which are formed by drawing silicon-oxygen tetrahedron and aluminum-oxygen octahedron, have good dispersibility and ion exchange performance, and can be used for adsorbing mycotoxin generated by mold by utilizing the characteristic of non-uniform charge distribution of montmorillonite. Usually, montmorillonite products circulating in the market mostly have an adsorption effect on aflatoxin, an adsorption effect on other mycotoxins is not obvious, and the defects of amino acid adsorption, vitamin adsorption and the like exist.
Disclosure of Invention
The invention provides a preparation method of an antibacterial mycotoxin adsorbing nano montmorillonite feed additive. The main purpose is to solve the defects of the prior art in the current market, overcome the defects of single mycotoxin adsorption, no antibiosis, nutrient element adsorption and the like of the existing product, and effectively inhibit the growth of harmful bacteria, adsorb mycotoxin and do not adsorb nutrient elements.
The technical scheme adopted by the invention is as follows:
a preparation method of an antibacterial mycotoxin adsorbing nano montmorillonite feed additive is characterized by comprising the following components in percentage by weight: 40-50% of nano montmorillonite, 1-5% of zinc oxide, 4-6% of yeast cell wall, 5-8% of bacillus subtilis freeze-dried powder, 4-5% of vitamin C, 10-15% of propionic acid/propionate and 7-11% of sodium diacetate.
Preferably, the preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive comprises the following components in percentage by weight: 48% of nano montmorillonite, 2.5% of zinc oxide, 5% of yeast cell wall, 7% of bacillus subtilis freeze-dried powder, 4.5% of vitamin C, 14% of propionic acid/propionate and 10% of sodium diacetate.
The preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive comprises the following steps:
respectively and uniformly mixing the nano-montmorillonite, the zinc oxide, the yeast cell wall, the bacillus subtilis freeze-dried powder, the vitamin C, the propionic acid/propionate and the sodium diacetate in proportion by a feed additive mixing unit to obtain the feed additive.
The preparation process method of the bacillus subtilis freeze-dried powder is realized by the following process means:
(1) fermenting and culturing the bacillus subtilis by using a fermentation culture medium, wherein the formula of the culture medium is as follows (%): corn flour 3, soybean cake powder 2 (hydrolysate, hydrolyzed with 0.5% NaOH at 121 deg.C for 30min, cooled, and filtered), peptone 0.2, and KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06 percent, NaCl 0.2 percent, glucose 0.5 percent, Tween-800.03, pH8.0, and culturing for 48 hours at the constant temperature of 37 ℃.
(2) After fermentation, the fermentation liquor is aseptically loaded into a centrifuge, the fermentation liquor is centrifuged for 5 min at 10000 rpm/min at 25 ℃, the supernatant is discarded, the obtained centrifugal precipitate is bacterial sludge, the bacterial sludge is resuspended into suspension by 1% peptone water solution, and the concentration of the bacillus subtilis is about 109 cfu/mL。
(3) Injecting the bacillus subtilis liquid prepared in the step (2) into an atomizer for atomization, wherein the air pressure during atomization is 0.72 kgf/cm2The liquid pressure was 0.45 kgf/cm2。
(4) The atomized liquid droplets are put into a collecting tank filled with a proper amount of calcium chloride solution (0.55 mol/L), the calcium chloride solution is gently stirred by a magnetic stirrer to harden for 20 min during the collecting process, then the calcium chloride solution is filtered by a high-density screen, and is washed twice by distilled water, and then the calcium chloride solution is transferred into a lactic acid solution (chitosan, for example) (chitosan C1.0% (w/v) and lactic acid 0.5% (v/v)), and the surface of the alginate microcapsules is evenly coated by gentle stirring for 10 min.
(5) And (3) filtering and washing the microcapsule prepared in the step (4), freeze-drying at-72 ℃ for 6 h, and then drying at-75 ℃ for 18 h, wherein the pressure during drying is set to be 5.28 Pa, so that the bacillus subtilis freeze-dried powder is obtained.
The invention modifies high-purity hydrated aluminosilicate to enhance the exchange positioning adsorption capacity of electrons between layers, can selectively adsorb mycotoxin in feed and animal bodies, fixes the mycotoxin to lose pathogenicity, and discharges the mycotoxin out of the bodies along with the peristalsis of large intestines, thereby avoiding intestinal mucosa injury. And does not adsorb nutrient substances such as vitamins and trace elements. The yeast cell wall and bacillus subtilis freeze-dried powder components in the invention have good effects of removing mycotoxin and harmful bacteria, maintaining intestinal health and improving animal immunity. The components of the propionic acid/propionate and the sodium diacetate have obvious effect on inhibiting the growth and proliferation of the mould.
The invention has the obvious effects of effectively preventing and treating livestock diarrhea, inhibiting mold proliferation, adsorbing and degrading mycotoxin, reducing the influence of the mycotoxin on the growth of livestock, improving the balance of intestinal flora of the livestock, improving the growth performance of the livestock and effectively reducing the use of antibiotics in the livestock and poultry breeding process.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described with reference to the following embodiments:
the embodiment I provides a preparation method of an antibacterial mycotoxin adsorbing nano montmorillonite feed additive, which comprises the following components in percentage by weight: 40% of nano montmorillonite, 5% of zinc oxide, 6% of yeast cell wall, 7% of bacillus subtilis freeze-dried powder, 5% of vitamin C, 15% of propionic acid/propionate and 11% of sodium diacetate.
The nanometer montmorillonite has a particle size of less than or equal to 100nm, can completely pass through a 325-mesh sieve, and has a specific surface area of more than or equal to 240m2/g。
The content of mannan in the yeast cell wall is 28%, the content of glucan is 25%, and the content of water-soluble protein is more than or equal to 30%.
1. The preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive comprises the following steps:
(1) fermenting and culturing the bacillus subtilis by using a fermentation culture medium, wherein the formula of the culture medium is as follows (%): corn flour 3, soybean cake powder 2 (hydrolysate, hydrolyzed with 0.5% NaOH at 121 deg.C for 30min, cooled, and filtered), peptone 0.2, and KH2PO4 0.15, K2HPO40.15、NaSO40.15、CaCl20.03, MnSO4 0.01、MgSO40.06 percent, NaCl 0.2 percent, glucose 0.5 percent, Tween-800.03, pH8.0, and culturing for 48 hours at the constant temperature of 37 ℃.
(2) After fermentation, the fermentation liquor is aseptically loaded into a centrifuge, the fermentation liquor is centrifuged for 5 min at 10000 rpm/min at 25 ℃, the supernatant is discarded, the obtained centrifugal precipitate is bacterial sludge, the bacterial sludge is resuspended into suspension by 1% peptone water solution, and the concentration of the bacillus subtilis is about 109 cfu/mL。
(3) Injecting the bacillus subtilis liquid prepared in the step 2) into an atomizer for atomization, wherein the air pressure during atomization is 0.72 kgf/cm2The liquid pressure was 0.45 kgf/cm2。
(4) The atomized liquid droplets are put into a collecting tank filled with a proper amount of calcium chloride solution (0.55 mol/L), the calcium chloride solution is gently stirred by a magnetic stirrer to harden for 20 min during the collecting process, then the calcium chloride solution is filtered by a high-density screen, and is washed twice by distilled water, and then the calcium chloride solution is transferred into a lactic acid solution (chitosan, for example) (chitosan C1.0% (w/v) and lactic acid 0.5% (v/v)), and the surface of the alginate microcapsules is evenly coated by gentle stirring for 10 min.
(5) And (3) filtering and washing the microcapsule prepared in the step (4), freeze-drying at-72 ℃ for 6 h, and then drying at-75 ℃ for 18 h, wherein the pressure during drying is set to be 5.28 Pa, so that the bacillus subtilis freeze-dried powder is obtained.
2. Uniformly mixing the nano montmorillonite, zinc oxide, yeast cell walls, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionate and sodium diacetate in proportion by a feed additive mixing unit to obtain the feed additive.
Embodiment two, a preparation method of an antibacterial mycotoxin adsorbing nano montmorillonite feed additive, which comprises the following components in percentage by weight: 44% of nano-montmorillonite, 2.5% of zinc oxide, 5% of yeast cell wall, 5% of bacillus subtilis freeze-dried powder, 4.5% of vitamin C, 13% of propionic acid/propionate and 9% of sodium diacetate.
The nanometer montmorillonite has a particle size of less than or equal to 100nm, can completely pass through a 325-mesh sieve, and has a specific surface area of more than or equal to 240m2/g。
The content of mannan in the yeast cell wall is 28%, the content of glucan is 25%, and the content of water-soluble protein is more than or equal to 30%.
1. The preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive comprises the following steps:
(1) fermenting and culturing the bacillus subtilis by using a fermentation culture medium, wherein the formula of the culture medium is as follows (%): corn flour 3, soybean cake powder 2 (hydrolysate, hydrolyzed with 0.5% NaOH at 121 deg.C for 30min, cooled, and filtered), peptone 0.2, and KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06 percent, NaCl 0.2 percent, glucose 0.5 percent, Tween-800.03, pH8.0, and culturing for 48 hours at the constant temperature of 37 ℃.
(2) After fermentation, the fermentation liquor is aseptically loaded into a centrifuge, the fermentation liquor is centrifuged for 5 min at 10000 rpm/min at 25 ℃, the supernatant is discarded, the obtained centrifugal precipitate is bacterial sludge, the bacterial sludge is resuspended into suspension by 1% peptone water solution, and the concentration of the bacillus subtilis is about 109 cfu/mL。
(3) Injecting the bacillus subtilis liquid prepared in the step (2) into an atomizer for atomization, wherein the air pressure during atomization is 0.72 kgf/cm2Pressure of liquidIs 0.45 kgf/cm2。
(4) The atomized liquid droplets are put into a collecting tank filled with a proper amount of calcium chloride solution (0.55 mol/L), the calcium chloride solution is gently stirred by a magnetic stirrer to harden for 20 min during the collecting process, then the calcium chloride solution is filtered by a high-density screen, and is washed twice by distilled water, and then the calcium chloride solution is transferred into a lactic acid solution (chitosan, for example) (chitosan C1.0% (w/v) and lactic acid 0.5% (v/v)), and the surface of the alginate microcapsules is evenly coated by gentle stirring for 10 min.
(5) And (3) filtering and washing the microcapsule prepared in the step (4), freeze-drying at-72 ℃ for 6 h, and then drying at-75 ℃ for 18 h, wherein the pressure during drying is set to be 5.28 Pa, so that the bacillus subtilis freeze-dried powder is obtained.
2. Uniformly mixing the nano montmorillonite, zinc oxide, yeast cell walls, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionate and sodium diacetate in proportion by a feed additive mixing unit to obtain the feed additive.
The third embodiment is a preparation method of an antibacterial mycotoxin adsorbing nano montmorillonite feed additive, which comprises the following components in percentage by weight: 48% of nano montmorillonite, 2.5% of zinc oxide, 5% of yeast cell wall, 7% of bacillus subtilis freeze-dried powder, 4.5% of vitamin C, 14% of propionic acid/propionate and 10% of sodium diacetate.
The nanometer montmorillonite has a particle size of less than or equal to 100nm, can completely pass through a 325-mesh sieve, and has a specific surface area of more than or equal to 240m2/g。
The content of mannan in the yeast cell wall is 28%, the content of glucan is 25%, and the content of water-soluble protein is more than or equal to 30%.
1. The preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive comprises the following steps:
(1) fermenting and culturing the bacillus subtilis by using a fermentation culture medium, wherein the formula of the culture medium is as follows (%): corn flour 3, soybean cake powder 2 (hydrolysate, hydrolyzed with 0.5% NaOH at 121 deg.C for 30min, cooled, and filtered), peptone 0.2, and KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06 percent, NaCl 0.2 percent, glucose 0.5 percent, Tween-800.03, pH8.0, and culturing for 48 hours at the constant temperature of 37 ℃.
(2) After fermentation, the fermentation liquor is aseptically loaded into a centrifuge, the fermentation liquor is centrifuged for 5 min at 10000 rpm/min at 25 ℃, the supernatant is discarded, the obtained centrifugal precipitate is bacterial sludge, the bacterial sludge is resuspended into suspension by 1% peptone water solution, and the concentration of the bacillus subtilis is about 109 cfu/mL。
(3) Injecting the bacillus subtilis liquid prepared in the step (2) into an atomizer for atomization, wherein the air pressure during atomization is 0.72 kgf/cm2The liquid pressure was 0.45 kgf/cm2。
(4) The atomized liquid droplets are put into a collecting tank filled with a proper amount of calcium chloride solution (0.55 mol/L), the calcium chloride solution is gently stirred by a magnetic stirrer to harden for 20 min during the collecting process, then the calcium chloride solution is filtered by a high-density screen, and is washed twice by distilled water, and then the calcium chloride solution is transferred into a lactic acid solution (chitosan, for example) (chitosan C1.0% (w/v) and lactic acid 0.5% (v/v)), and the surface of the alginate microcapsules is evenly coated by gentle stirring for 10 min.
(5) And (3) filtering and washing the microcapsule prepared in the step (4), freeze-drying at-72 ℃ for 6 h, and then drying at-75 ℃ for 18 h, wherein the pressure during drying is set to be 5.28 Pa, so that the bacillus subtilis freeze-dried powder is obtained.
2. Uniformly mixing the nano montmorillonite, zinc oxide, yeast cell walls, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionate and sodium diacetate in proportion by a feed additive mixing unit to obtain the feed additive.
The fourth embodiment is a preparation method of an antibacterial mycotoxin adsorbing nano montmorillonite feed additive, which comprises the following components in percentage by weight: 50% of nano montmorillonite, 1% of zinc oxide, 4% of yeast cell wall, 8% of bacillus subtilis freeze-dried powder, 4% of vitamin C, 10% of propionic acid/propionate and 7% of sodium diacetate.
The nanometer montmorillonite has a particle size of less than or equal to 100nm, can completely pass through a 325-mesh sieve, and has a specific surface area of more than or equal to 240m2/g。
The content of mannan in the yeast cell wall is 28%, the content of glucan is 25%, and the content of water-soluble protein is more than or equal to 30%.
1. The preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive comprises the following steps:
(1) fermenting and culturing the bacillus subtilis by using a fermentation culture medium, wherein the formula of the culture medium is as follows (%): corn flour 3, soybean cake powder 2 (hydrolysate, hydrolyzed with 0.5% NaOH at 121 deg.C for 30min, cooled, and filtered), peptone 0.2, and KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06 percent, NaCl 0.2 percent, glucose 0.5 percent, Tween-800.03, pH8.0, and culturing for 48 hours at the constant temperature of 37 ℃.
(2) After fermentation, the fermentation liquor is aseptically loaded into a centrifuge, the fermentation liquor is centrifuged for 5 min at 10000 rpm/min at 25 ℃, the supernatant is discarded, the obtained centrifugal precipitate is bacterial sludge, the bacterial sludge is resuspended into suspension by 1% peptone water solution, and the concentration of the bacillus subtilis is about 109 cfu/mL。
(3) Injecting the bacillus subtilis liquid prepared in the step (2) into an atomizer for atomization, wherein the air pressure during atomization is 0.72 kgf/cm2The liquid pressure was 0.45 kgf/cm2。
(4) The atomized liquid droplets are put into a collecting tank filled with a proper amount of calcium chloride solution (0.55 mol/L), the calcium chloride solution is gently stirred by a magnetic stirrer to harden for 20 min during the collecting process, then the calcium chloride solution is filtered by a high-density screen, and is washed twice by distilled water, and then the calcium chloride solution is transferred into a lactic acid solution (chitosan, for example) (chitosan C1.0% (w/v) and lactic acid 0.5% (v/v)), and the surface of the alginate microcapsules is evenly coated by gentle stirring for 10 min.
(5) And (3) filtering and washing the microcapsule prepared in the step (4), freeze-drying at-72 ℃ for 6 h, and then drying at-75 ℃ for 18 h, wherein the pressure during drying is set to be 5.28 Pa, so that the bacillus subtilis freeze-dried powder is obtained.
2. Uniformly mixing the nano montmorillonite, zinc oxide, yeast cell walls, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionate and sodium diacetate in proportion by a feed additive mixing unit to obtain the feed additive.
The specific experimental verification is as follows:
experimental example 1 nutrient adsorption experiment
Taking a formula product of the invention in the embodiment 3 for experiment, taking 10 triangular bottles with the number of 1-10, accurately weighing 1g of each experimental sample, adding the experimental sample into the numbered triangular bottles, respectively dissolving the experimental sample with 10mL of pure water of Waohaha, respectively adding 0.01g of VB1, VB2, VB6, VB12 and methionine into the numbered triangular bottles with the number of 1-5, respectively adding the common montmorillonite sample equivalent to the invention into the numbered triangular bottles with the number of 6-10 and dissolving the 10mL of Waohaha in pure water, sequentially adding 0.01g of VB1, VB2, VB6, VB12 and methionine, treating the mixture for 3 hours at constant temperature of 25 ℃ and 100r/min, and respectively measuring the content changes of VB1, VB2, VB6, VB12 and methionine, wherein the experimental results are shown in Table 1.
TABLE 1 adsorption of the invention to various vitamins and methionine
| Nutrient species | Products of the invention (g) | Common montmorillonite (g) |
| VB1 | 0.0098 | 0.0045 |
| VB2 | 0.0099 | 0.0058 |
| VB6 | 0.0095 | 0.0061 |
| VB12 | 0.0097 | 0.0032 |
| Methionine | 0.009 | 0.0024 |
As can be seen from the results in Table 1, the invention has little adsorption to vitamins and amino acids, basically no adsorption, and the application of the product of the invention does not cause the problem of adsorption to nutrient elements.
Experimental example 2, experiment of mold prevention and mold removal effects
The product prepared according to the formula of the invention in the embodiment 3 is used for experiments, the product is uniformly mixed with 50 kg of feed raw materials according to the addition amount of 500g/T, the product is not added in a control group, the warehouse temperature is kept at 25 ℃ and the humidity is kept at 60 percent for 1 month, the peculiar smell, the temperature and the microorganism conditions of each experimental product are detected, and the test results are shown in table 2.
TABLE 2 mildew-proof effect test of the product of the invention on feed materials
| Test item | Experimental group | Control group |
| Peculiar smell | Is free of | Has a musty taste |
| Temperature of | 25℃-30℃ | 30℃-45℃ |
| Aspergillus flavus | Not detected out | 0.5×104cfu/g |
| Fusarium | Not detected out | 1×106cfu/g |
| Escherichia coli | 1×103cfu/g | 2.01×106cfu/g |
| Clostridium botulinum | Not detected out | 1×104cfu/g |
As can be seen from the test results in Table 2, the product of the invention can effectively prevent feed raw materials from mildewing, has obvious inhibition effect on the proliferation of aspergillus flavus, fusarium, clostridium botulinum and escherichia coli, and has good effect on feed raw material mildew prevention.
Taking a formula product of the embodiment 3 of the invention for experiment, adding the formula product into 100g of feed containing 100ppb aflatoxin, 500ppb zearalenone and 1000ppb vomitoxin, mixing uniformly, putting the mixture into a 250mL triangular flask, adding 150mL sterile water for dissolving, treating for 2.5h at constant temperature of 25 ℃ and 100r/min, and respectively determining the contents of aflatoxin, zearalenone and vomitoxin, wherein the test result is shown in Table 3:
TABLE 3 Effect of the products of the invention on the removal of the major toxins from the feed
| Test item | Before the experiment | After the test | Mycotoxin removal rate |
| Aflatoxin | 100ppb | 1ppb | 99% |
| Zearalenone | 500ppb | 101.96ppb | 79.6% |
| Vomitoxin | 1000ppb | 245.69ppb | 75.43% |
The test results in table 3 show that the product of the invention has detoxification effects on aflatoxin, zearalenone and vomitoxin, and the removal rate of the product on the mycotoxin is 99% of aflatoxin, 79.6% of zearalenone and 75.43% of vomitoxin, so that the product of the invention has good effect on removing the mycotoxin.
The above experiments describe the specific embodiments of the present invention in detail, but the present invention is not limited to the embodiments, so that technical solutions and technical process modifications obtained by developers skilled in the art based on the technical idea of the present invention belong to the protection scope of the present invention.
Claims (4)
1. The preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive is characterized by comprising the following components in percentage by weight: 40-50% of nano montmorillonite, 1-5% of zinc oxide, 4-6% of yeast cell wall, 5-8% of bacillus subtilis freeze-dried powder, 4-5% of vitamin C, 10-15% of propionic acid/propionate and 7-11% of sodium diacetate.
2. The preparation method of the antibacterial mycotoxin adsorbing nano-montmorillonite feed additive according to claim 1, characterized by comprising the following components in percentage by weight: 48% of nano montmorillonite, 2.5% of zinc oxide, 5% of yeast cell wall, 7% of bacillus subtilis freeze-dried powder, 4.5% of vitamin C, 14% of propionic acid/propionate and 10% of sodium diacetate.
3. The preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive as claimed in claims 1-2, wherein the nano montmorillonite has a particle size of less than or equal to 100nm, completely passes through a 325-mesh sieve, and has a specific surface area of more than or equal to 240m2/g。
4. The preparation method of the antibacterial mycotoxin adsorbing nano montmorillonite feed additive as claimed in claims 1-2, wherein the content of mannan and glucan in the yeast cell wall are 28%, 25% and the content of water-soluble protein is more than or equal to 30%.
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