CN111493322B - Microencapsulated probiotic bacteria and preparation method and application thereof - Google Patents

Microencapsulated probiotic bacteria and preparation method and application thereof Download PDF

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Publication number
CN111493322B
CN111493322B CN202010414336.8A CN202010414336A CN111493322B CN 111493322 B CN111493322 B CN 111493322B CN 202010414336 A CN202010414336 A CN 202010414336A CN 111493322 B CN111493322 B CN 111493322B
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microencapsulated probiotic
core material
probiotic bacteria
microencapsulated
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CN111493322A (en
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朱蒙蒙
朱永亮
王一伟
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Suzhou Preyson Biotechnology Co ltd
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Suzhou Preyson Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
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    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
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    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3526—Organic compounds containing nitrogen
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
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    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
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    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
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    • A23L3/3562—Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/30—Encapsulation of particles, e.g. foodstuff additives
    • A—HUMAN NECESSITIES
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    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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    • Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation

Abstract

The invention relates to a microencapsulated probiotic and a preparation method and application thereof, wherein the microencapsulated probiotic comprises a core material and a wall material; the core material comprises a composite microbial inoculum, prebiotics and skimmed milk powder; the wall material comprises polypeptide, antioxidant and modified cellulose. The core material of the microencapsulated probiotics is formed by compounding a composite microbial inoculum, prebiotics and skimmed milk powder, and the composite microbial inoculum, the prebiotics and the skimmed milk powder supplement each other, so that the probiotics have the effects of regulating intestinal flora, improving the immunity of the organism, and promoting intestinal digestion and nutrient absorption; the wall material of the microencapsulated probiotics is creatively composed of polypeptide, antioxidant and modified cellulose, can well obstruct external stress, ensure the tolerance stability of the product, can be planted in intestinal tracts efficiently, can promote the functions of regulating intestinal flora and improving the immunity of organisms, and can prolong the shelf life of the product.

Description

Microencapsulated probiotic bacteria and preparation method and application thereof
Technical Field
The invention belongs to the technical field of biology, relates to probiotics and a preparation method and application thereof, and particularly relates to microencapsulated probiotics and a preparation method and application thereof.
Background
Probiotics is a kind of active microorganisms beneficial to a host, and is a general term for active beneficial microorganisms which are planted in the intestinal tract and the reproductive system of a human body and can generate definite health efficacy so as to improve the micro-ecological balance of the host and play beneficial roles. It is often used as a beneficial live bacterial agent for oral administration, either alone or in combination with other food ingredients.
Prebiotics are carbohydrates that are not digested and absorbed by the human gut and are used as "food" for probiotics, which promote the growth and activity of probiotics, especially in the lower gut, such as the colon, and produce a synergistic effect that promotes intestinal health.
CN106306363A compound probiotic formula and production process thereof, which comprises the following raw materials by weight: 5-10% of enterococcus faecalis, 1-2% of bacillus subtilis, 1-2% of bacillus licheniformis, 2-4% of clostridium butyricum, 5-10% of saccharomyces cerevisiae, 5-20% of fructooligosaccharide and the balance of glucose. The invention has simple production process, can effectively improve the specificity and non-specificity immunity of animal organisms, enhance the disease resistance of the organisms, improve the breeding environment, reduce harmful gases such as ammonia gas and the like, remove mycotoxin, reduce the harm caused by mildew of feed, improve the quality of animal products and improve the breeding benefit.
CN105815640A fruit and vegetable probiotics with functions of promoting digestion and regulating intestines and stomach and a preparation method thereof, sugar powder in fruits and vegetables is fully converted into probiotics thallus and metabolites by adopting a two-stage fermentation method for the fruits and vegetables such as cumquats, hawthorns, apples, lemons, bananas, pineapples, grapes, pears, sea buckthorn fruits, kiwi fruits, pumpkins, white radishes, carrots and the like which have the functions of regulating intestines and stomach and promote digestion, and a large amount of vitamins, trace elements and metabolic organic acids in the fruits and vegetables are reserved; the concentration of organic acid is adjusted by calcium carbonate and converted into organic calcium, so that the organic calcium is beneficial to intestinal absorption and eliminates the feeling of acidity and astringency; the xylitol and the prebiotics are adopted to regulate sweet taste, so that calcium absorption can be enhanced, and the proliferation of probiotics in vivo and the colonization effect of exogenous probiotics can be enhanced.
Reports on how to better and more reasonably select probiotics for cooperation and synergy on improving intestinal flora and improving immunity are rarely reported in the prior art.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide probiotics and a preparation method and application thereof, and particularly provides microencapsulated probiotics and a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
in one aspect, the present invention provides a microencapsulated probiotic, comprising a core material and a wall material; the core material comprises a composite microbial inoculum, prebiotics and skimmed milk powder; the wall material comprises polypeptide, antioxidant and modified cellulose.
The core material of the microencapsulated probiotics is compounded by the composite microbial inoculum, the prebiotics and the skimmed milk powder, and the composite microbial inoculum, the prebiotics and the skimmed milk powder supplement each other, so that the probiotics have the effects of regulating intestinal flora, improving the immunity of the organism, and promoting intestinal digestion and nutrient absorption; it can significantly reduce the content of harmful bacteria such as Escherichia coli in intestinal tract, and significantly increase beneficial bacteria such as lactobacillus and Bacillus bifidus. The wall material of the microencapsulated probiotics is creatively composed of polypeptide, antioxidant and modified cellulose, can well obstruct external stress, ensure the tolerance stability of the product, can be planted in intestinal tracts efficiently, can promote the exertion of the effects and prolong the shelf life of the product.
Preferably, the complex microbial inoculum comprises bifidobacterium lactis, lactobacillus plantarum and lactobacillus rhamnosus.
The composite microbial inoculum is specifically compounded by bifidobacterium lactis, lactobacillus plantarum and lactobacillus rhamnosus, wherein the lactobacillus plantarum can be efficiently planted in intestinal tracts, inhibit the propagation of pathogenic bacteria, help to proliferate beneficial bacteria in the intestinal tracts, regulate the balance of intestinal flora and improve the immunity; the Bifidobacterium lactis has effects of improving gastrointestinal barrier function, regulating intestinal flora, relieving constipation, relieving diarrhea caused by antibiotics, enhancing immunity, improving resistance of organism to respiratory tract infection, and resisting allergy; the lactobacillus rhamnosus has the functions of balancing intestinal flora and enhancing the nonspecific cellular immunity of organisms through the phagocytosis of macrophages; the action sites and the action modes of the three materials supplement each other, and the synergistic effect is realized, so that the excellent effects of regulating intestinal flora, improving the immunity of the organism and promoting the digestion of intestinal tracts and the absorption of nutrients are jointly exerted.
Preferably, the bifidobacterium lactis comprises bifidobacterium lactis Bb-12.
Preferably, the lactobacillus plantarum comprises lactobacillus plantarum N13 and/or lactobacillus plantarum CW006.
Preferably, the lactobacillus rhamnosus comprises lactobacillus rhamnosus LRa05 and/or lactobacillus rhamnosus HN001.
As a preferred technical scheme of the invention, the bifidobacterium lactis, the lactobacillus plantarum and the lactobacillus rhamnosus are matched by selecting the specific types.
Preferably, the complex microbial inoculum comprises 50-70 parts of bifidobacterium lactis Bb-12, 30-50 parts of lactobacillus plantarum N13, 10-30 parts of lactobacillus plantarum CW006, 30-50 parts of lactobacillus rhamnosus LRa05 and 30-50 parts of lactobacillus rhamnosus HN001 in parts by weight.
When the bifidobacterium lactis, the lactobacillus plantarum and the lactobacillus rhamnosus related by the invention adopt the specific mass ratio, the beneficial effects of the product can be optimized.
The weight parts of the bifidobacterium lactis Bb-12 can be 50 parts, 55 parts, 60 parts, 65 parts or 70 parts, and other specific point values in the range can be selected, and are not repeated.
The lactobacillus plantarum N13 can be 30 parts, 35 parts, 40 parts, 45 parts, 50 parts or the like by weight, and other specific point values within the above range can be selected, so that the details are not repeated.
The lactobacillus plantarum CW006 can be 10 parts, 15 parts, 20 parts, 25 parts or 30 parts by weight, and other specific values in the above range can be selected, so that the details are not repeated.
The lactobacillus rhamnosus LRa05 can be 30 parts, 35 parts, 40 parts, 45 parts or 50 parts by weight, and other specific values in the range can be selected, so that the details are not repeated.
The weight portion of the lactobacillus rhamnosus HN001 can be 30, 35, 40, 45 or 50, and other specific point values in the range can be selected, and are not repeated herein.
The activity range of the probiotics in the probiotics related to the invention is 1 multiplied by 10 10 cfu/g-1×10 12 cfu/g, e.g. 1X 10 10 cfu/g、5×10 10 cfu/g、1×10 11 cfu/g、5×10 11 cfu/g or 1X 10 12 cfu/g, and other specific point values within the above range can be selected, and are not described in detail herein.
Preferably, the prebiotic comprises a fructooligosaccharide and/or a galactooligosaccharide.
The invention adopts fructo-oligosaccharide and/or galacto-oligosaccharide as prebiotic components to be combined with the probiotics, which is beneficial to the survival and propagation of the probiotics.
Preferably, the core material further comprises a binder and/or polydextrose.
Preferably, the binder comprises any one or a combination of at least two of maltodextrin, soy protein, pea protein, cyclodextrin, gellan gum, acacia gum or corn syrup. The combination of at least two of the above-mentioned compounds, such as the combination of maltodextrin and soybean protein, the combination of maltodextrin and cyclodextrin, the combination of acacia gum and corn syrup, etc., can be selected in any combination manner, and thus, the details are not repeated herein.
In the present invention, the weight ratio of the core material to the wall material is 1:2-2:1, for example, 1:2, 2:3, 1:1, 3:2, or 2:1, etc., and other specific values in the above range can be selected, which are not described herein again.
Preferably, the polypeptide comprises a soy polypeptide and/or a fish collagen peptide, preferably a combination of a soy polypeptide and a fish collagen peptide.
Preferably, the weight ratio of the soybean polypeptide to the fish collagen peptide is (1-3): 1, for example, 1:1, 1.5.
According to the invention, the soybean polypeptide and the fish collagen peptide are used as the combination of the polypeptides and added into the wall material to be combined and matched with the modified cellulose, so that the tolerance stability and the intestinal tract planting capability of the product can be obviously ensured, the effect exertion of the product is promoted, and the fish collagen peptide has a certain antioxidant property and the shelf life of the product is prolonged. The scheme of which the weight ratio is (1-3) to 1 is the scheme which enables the effect to be better.
Preferably, the antioxidant comprises vitamin E and/or rosemary, preferably a combination of vitamin E and rosemary.
Preferably, the weight ratio of the vitamin E to the rosemary is (3-4): 1, for example, 3:1, 3.2.
According to the invention, vitamin E and rosemary are used as antioxidant compounds and added into the wall material, so that the product has no rancidity-like smell, and the quality guarantee period and the shelf life of the product are obviously prolonged. The scheme with the weight ratio of (3-4) to 1 is the scheme which enables the effect to be better.
Preferably, the modified cellulose comprises sodium carboxymethyl cellulose and/or hydroxypropyl methyl cellulose, preferably a combination of sodium carboxymethyl cellulose and hydroxypropyl methyl cellulose.
Preferably, the weight ratio of the sodium carboxymethyl cellulose to the hydroxypropyl methyl cellulose is 1 (4-6), for example, 1:4, 1.
In another aspect, the present invention provides a method for preparing microencapsulated probiotics, comprising:
mixing core material raw materials to prepare a core material, drying and sieving the core material, and embedding the core material by using a wall material solution to obtain the microencapsulated probiotic bacteria.
Preferably, the core material is prepared by a centrifugal pelleting method, an extrusion spheronization method or a swing granulation method.
In the centrifugal pelleting method, the temperature is 25-30 ℃ (for example, 25 ℃, 28 ℃ or 30 ℃ and the like), the rotation speed of the pelleting turntable does not exceed 400rpm (for example, 100rpm, 200rpm, 300rpm or 400rpm and the like), other specific points in the range can be selected, and the detailed description is omitted.
In the extrusion spheronization method, the temperature is 30-35 ℃ (for example, 30 ℃, 31 ℃, 32 ℃, 33 ℃ or 35 ℃, and the like), the extruded particle size is 0.8-1.2mm (for example, 0.8mm, 0.9mm, 1.0mm, 1.1mm or 1.2mm, and the like), and the revolution number of a spheronization turntable is not more than 1000rpm (for example, 700rpm, 800rpm, 900rpm, 1000rpm, and the like); other specific point values within the above range can be selected, and are not described in detail herein.
In the rocking granulation method, the temperature is 25-30 ℃ (e.g. 25 ℃, 28 ℃ or 30 ℃, etc.), the drum rotation number is 70-80rpm (e.g. 70rpm, 72rpm, 75rpm, 78rpm or 80rpm, etc.); other specific point values within the above range can be selected, and are not described in detail herein.
Preferably, the sieving refers to sieving with a 40-70 mesh sieve, such as 40 mesh, 45 mesh, 50 mesh, 55 mesh, 60 mesh, 65 mesh or 70 mesh, and other specific values within the above range can be selected, and are not repeated herein.
Preferably, the concentration of the wall material solution is 60-80%, such as 60%, 65%, 70%, 75%, or 80%, and other specific values within the above range can be selected, which is not described herein again.
Preferably, the embedding is carried out by using a coating machine, the inlet air temperature is 60-70 ℃ (for example, 60 ℃, 62 ℃, 65 ℃, 67 ℃ or 70 ℃ and the like), the outlet air temperature is 30-35 ℃ (for example, 30 ℃, 31 ℃, 32 ℃, 34 ℃ or 35 ℃ and the like), the temperature in the coating cavity is 48-50 ℃ (for example, 48 ℃, 48.5 ℃, 49 ℃, 49.5 ℃ or 50 ℃ and the like), and the spray liquid revolution is 5-10rpm (for example, 5rpm, 6rpm, 7rpm, 8rpm, 9rpm or 10rpm and the like).
In a further aspect, the present invention provides a use of the microencapsulated probiotic bacteria as described above for the preparation of a probiotic food or health product.
Compared with the prior art, the invention has the following beneficial effects:
the core material of the microencapsulated probiotics is compounded by the composite microbial inoculum, the prebiotics and the skim milk powder, and the composite microbial inoculum, the prebiotics and the skim milk powder supplement each other, so that the probiotics have the effects of regulating intestinal flora, improving the immunity of organisms and promoting intestinal digestion and nutrient absorption, and the product is added into daily ration of weaned pigs as an additive, so that the feed-meat ratio is not higher than 1.51 +/-0.10, and the diarrhea rate is not higher than 8.0 +/-0.11. Compared with common daily ration, the daily ration added with the product can reduce the number of escherichia coli in the intestinal tract from 6.47 +/-0.07 to below 4.06 +/-0.05, increase the number of lactobacillus from 7.06 +/-0.12 to above 8.22 +/-0.06 and increase the number of bifidobacterium from 7.20 +/-0.08 to above 8.04 +/-0.20. The wall material of the microencapsulated probiotics is creatively composed of polypeptide, antioxidant and modified cellulose, can well obstruct external stress, ensure the tolerance stability of the product, can be planted in intestinal tracts efficiently, can promote the exertion of the effects and prolong the shelf life of the product.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The fructooligosaccharides referred to in the following examples were obtained from Sirana Baichuan Biotech Co.Ltd; galacto-oligosaccharides were purchased from zhengzhou yu and food additives ltd; the skimmed milk powder is purchased from David Biotechnology Co., ltd; polydextrose was purchased from Zhengzhou Yu and food additives Co., ltd; soybean polypeptide was purchased from sienna baichuan biotechnology limited; fish collagen peptide was purchased from Zhengzhou Yu and food additives Co., ltd.
Example 1
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material is prepared from 60 parts of bifidobacterium lactis Bb-12, 40 parts of lactobacillus plantarum N13, 20 parts of lactobacillus plantarum CW006, 40 parts of lactobacillus rhamnosus LRa05, 40 parts of lactobacillus rhamnosus HN001, 20 parts of fructo-oligosaccharide, 20 parts of galacto-oligosaccharide, 10 parts of skimmed milk powder, 30 parts of maltodextrin and 5 parts of polydextrose;
the wall material comprises 50 parts of soybean polypeptide, 20 parts of fish collagen peptide, 40 parts of vitamin E, 10 parts of rosemary, 20 parts of sodium carboxymethylcellulose and 80 parts of hydroxypropyl methyl cellulose.
The preparation method comprises the following steps:
(1) Mixing the raw materials of the core material according to a ratio, and forming a pellet shape in a centrifugal pellet mill at the temperature of 30 ℃ and the rotation speed of a pellet-making turntable of 300rpm;
(2) Drying the pellets, and sieving the pellets by a 50-mesh sieve;
(3) Mixing the wall materials to prepare a 70% aqueous solution, and coating the pellets in a coating machine in a bottom spraying manner, wherein the air inlet temperature is 60 ℃, the air outlet temperature is 35 ℃, the temperature in a coating cavity is 48 ℃, and the spraying speed is 5rpm; obtaining the microencapsulated probiotic bacteria.
Example 2
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material is prepared from 50 parts of bifidobacterium lactis Bb-12, 50 parts of lactobacillus plantarum N13, 10 parts of lactobacillus plantarum CW006, 50 parts of lactobacillus rhamnosus LRa05, 30 parts of lactobacillus rhamnosus HN001, 30 parts of fructo-oligosaccharide, 10 parts of galacto-oligosaccharide, 15 parts of skimmed milk powder and 40 parts of maltodextrin;
the wall material comprises 120 parts of soybean polypeptide 40 parts, fish collagen peptide 40 parts, vitamin E30 parts, rosemary 10 parts, sodium carboxymethylcellulose 20 parts and hydroxypropyl methyl cellulose.
The preparation method comprises the following steps:
(1) Mixing the raw materials of the core material according to a ratio, and forming a pellet shape in a centrifugal pellet mill at the temperature of 25 ℃ with the rotation speed of a pellet-making turntable of 400rpm;
(2) Drying the pellets, and sieving the pellets with a 60-mesh sieve;
(3) Mixing the wall materials to prepare a 75% aqueous solution, coating the pellets in a coating machine in a bottom spraying manner, wherein the air inlet temperature is 70 ℃, the air outlet temperature is 30 ℃, the temperature in a coating cavity is 50 ℃, and the spraying speed is 8rpm; obtaining the microencapsulated probiotic bacteria.
Example 3
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material is prepared from 70 parts of bifidobacterium lactis Bb-12, 30 parts of lactobacillus plantarum N13, 30 parts of lactobacillus plantarum CW006, 30 parts of lactobacillus rhamnosus LRa05, 50 parts of lactobacillus rhamnosus HN001, 20 parts of fructo-oligosaccharide, 30 parts of galacto-oligosaccharide, 10 parts of skimmed milk powder and 50 parts of maltodextrin;
the wall material comprises 150 parts of soybean polypeptide 50 parts, fish collagen peptide 20 parts, vitamin E40 parts, rosemary 10 parts, sodium carboxymethylcellulose 30 parts and hydroxypropyl methyl cellulose.
The preparation method comprises the following steps:
(1) Mixing the raw materials of the core material according to a ratio, and forming a pellet shape in a centrifugal pellet mill at the temperature of 30 ℃ and the rotation speed of a pellet-making turntable of 300rpm;
(2) Drying the pellets, and sieving the pellets by a 70-mesh sieve;
(3) Mixing the wall materials to prepare a 70% aqueous solution, and coating the pellets in a coating machine in a bottom spraying manner, wherein the air inlet temperature is 60 ℃, the air outlet temperature is 35 ℃, the temperature in a coating cavity is 48 ℃, and the spraying speed is 5rpm; obtaining the microencapsulated probiotic bacteria.
Example 4
The embodiment provides a microencapsulated probiotic bacteria, which comprises a core material and a wall material;
the core material differs from example 1 only in that: does not contain lactobacillus plantarum N13, and lactobacillus plantarum CW006 part, the others remain unchanged;
the wall material and the preparation method remain the same as in example 1.
Example 5
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material differs from example 1 only in that: does not contain the plant lactobacillus CW006, and the plant lactobacillus N13 shares, and the others remain unchanged;
the wall material and the preparation method were in accordance with example 1.
Example 6
The embodiment provides a microencapsulated probiotic bacteria, which comprises a core material and a wall material;
the core material differs from example 1 only in that: the lactobacillus rhamnosus LRa05 is not contained, the lactobacillus rhamnosus HN001 parts, and the others are kept unchanged;
the wall material and the preparation method remain the same as in example 1.
Example 7
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material differs from example 1 only in that: the lactobacillus rhamnosus HN001 is not contained, 80 parts of lactobacillus rhamnosus LRa05 are contained, and the rest parts are kept unchanged;
the wall material and the preparation method remain the same as in example 1.
Example 8
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material differs from example 1 only in that: does not contain bifidobacterium lactis Bb-12, and contains 13 parts of lactobacillus plantarum N, 29 parts of lactobacillus plantarum CW006, 58 parts of lactobacillus rhamnosus LRa05 and 55 parts of lactobacillus rhamnosus HN, and the rest of the components are kept unchanged;
the wall material and the preparation method remain the same as in example 1.
Example 9
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material differs from example 1 only in that: does not contain lactobacillus plantarum, and bifidobacterium lactis Bb-12 parts, lactobacillus rhamnosus LRa05 parts, lactobacillus rhamnosus HN001 parts, and the others are kept unchanged;
the wall material and the preparation method remain the same as in example 1.
Example 10
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material differs from example 1 only in that: the lactobacillus rhamnosus is not contained, and the bifidobacterium lactis Bb-12 parts, the lactobacillus plantarum N13 parts and the lactobacillus plantarum CW006 parts are kept unchanged, wherein the lactobacillus plantarum Bb-12 parts, the lactobacillus plantarum N65 parts and the lactobacillus plantarum CW006 parts are added;
the wall material and the preparation method were in accordance with example 1.
Example 11
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the core material is different from the core material in the embodiment 1 only in the difference of the mixture ratio of the bacteria in the composite microbial inoculum: 40 parts of bifidobacterium lactis Bb-12, 60 parts of lactobacillus plantarum N, 40 parts of lactobacillus plantarum CW006, 30 parts of lactobacillus rhamnosus LRa05 and 30 parts of lactobacillus rhamnosus HN001, and the rest of the components are kept unchanged;
the wall material and the preparation method remain the same as in example 1.
Example 12
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the wall material differs from example 1 only in that: the fish collagen peptide does not contain soybean polypeptide, 70 parts of fish collagen peptide and the rest parts of fish collagen peptide are kept unchanged;
the core material and the preparation method remain the same as in example 1.
Example 13
The embodiment provides a microencapsulated probiotic, which comprises a core material and a wall material;
the wall material only differs from example 1 in that: the fish collagen peptide is not contained, 70 parts of soybean polypeptide are contained, and the rest parts are kept unchanged;
the core material and preparation method remained the same as in example 1.
Comparative example 1
The present comparative example provides a microencapsulated probiotic, comprising a core material and a wall material;
the core material only differs from example 1 in that it does not contain prebiotics, i.e. fructooligosaccharides and galactooligosaccharides, the others remaining unchanged;
the wall material and the preparation method remain the same as in example 1.
Comparative example 2
The present comparative example provides a microencapsulated probiotic, comprising a core material and a wall material;
the core material only differs from example 1 in that it does not contain skimmed milk powder, the rest remaining unchanged;
the wall material and the preparation method remain the same as in example 1.
Comparative example 3
The present comparative example provides a microencapsulated probiotic, comprising a core material and a wall material;
the wall material only differs from example 1 in that it does not contain polypeptide components, i.e. soy polypeptide and fish collagen peptide, and the others remain unchanged;
the core material and the preparation method remain the same as in example 1.
Evaluation test:
(1) The invention adopts weaned piglets as experimental animal models, and measures corresponding indexes by adding the product of the invention into the daily ration for feeding. The method specifically comprises the following steps: 68 weaned pigs (weaned at 28 days old) with similar body weights are selected and randomly divided into 17 groups, namely test groups 1-16 and a control group, wherein 4 weaned pigs are fed by different daily rations, the test groups 1-16 are respectively prepared by adding the products of examples 1-13 and comparative examples 1-3 into basic daily rations, the addition amount is 5%, and the control group is the basic daily ration. After 28 days, feed-meat ratio evaluation (ratio of average daily feed intake (g) to average daily gain (g)), diarrhea rate evaluation (sum of number of diarrhea piglets per day in experimental period/(number of experimental piglets x experimental days) × 100%) and intestinal flora evaluation were performed.
(1.1) piglets were weighed on empty stomach on trial day 28, and the feed-meat ratio (F/G) and diarrhea rate were calculated, with the results shown in table 1 (final data presented as mean ± standard deviation).
TABLE 1
Figure BDA0002494382070000121
Figure BDA0002494382070000131
As can be seen from the data in Table 1: the product of the invention ensures that the feed-meat ratio is not higher than 1.51 +/-0.10 and the diarrhea rate is not higher than 8.0 +/-0.11, which indicates that the product has the effects of promoting digestion and absorption of nutrient components and can enhance the immunity of the organism from side view.
(1.2) after 28 days, slaughtering and accurately weighing the cecal intestine content of the pig, diluting with sterile normal saline to obtain 10mg/mL bacterial suspension with final concentration of 10mg/mL 0 The mother liquor was diluted by fold. Sequentially diluting to obtain 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Diluted bacterial liquid. And respectively inoculating 10 mu L of the diluted solution to 3 culture media for carrying out isolated culture on escherichia coli, lactobacillus and bifidobacterium, and detecting each dilution for 3 times. At the same time, physiological saline was added to the sterile plate as a blank. After 48h incubation the dishes were removed and the number of colonies was calculated and the results are shown in table 2 (final data are presented as mean ± standard deviation).
TABLE 2
Group of Escherichia coli (log CFU/g) Lactobacillus (log CFU/g) Bifidobacterium (log CFU/g)
Test group 1 1.78±0.21 14.75±0.04 12.82±0.11
Test group 2 1.93±0.01 12.66±0.12 11.98±0.06
Test group 3 1.85±0.05 13.43±0.15 12.32±0.09
Test group 4 2.46±0.18 12.55±0.05 11.75±0.12
Test group 5 1.92±0.14 11.24±0.14 12.07±0.08
Test group 6 1.84±0.12 11.74±0.18 11.88±0.11
Test group 7 2.52±0.06 13.96±0.21 10.52±0.03
Test group 8 3.67±0.08 10.69±0.15 9.12±0.09
Test group 9 4.06±0.05 9.34±0.18 9.85±0.14
Test group 10 2.88±0.15 8.22±0.06 8.04±0.20
Test group 11 2.35±0.09 13.65±0.08 11.42±0.18
Test group 12 1.83±0.08 14.15±0.11 12.45±0.12
Test group 13 1.89±0.04 14.38±0.05 12.37±0.06
Test group 14 3.07±0.11 10.93±0.17 10.19±0.07
Test group 15 2.53±0.08 14.04±0.06 12.25±0.13
Test group 16 1.90±0.12 14.16±0.16 11.62±0.16
Control group 6.47±0.07 7.06±0.12 7.20±0.08
As can be seen from the data in Table 2: the microencapsulated probiotics can regulate intestinal flora, obviously promote the quantity of beneficial intestinal flora such as bifidobacteria and lactobacillus, and obviously inhibit the quantity of harmful intestinal flora such as escherichia coli. Compared with a control group, the test group reduces the number of the Escherichia coli from 6.47 +/-0.07 to below 4.06 +/-0.05; compared with the control group, the test group increases the number of the lactobacillus from 7.06 +/-0.12 to more than 8.22 +/-0.06; compared with the control group, the test group increases the number of the bifidobacteria from 7.20 +/-0.08 to more than 8.04 +/-0.20.
(2) And (5) observing intestinal colonization capability. The specific operation method comprises the following steps: respectively weighing 0.5g of the products of examples 1-13 and comparative examples 1-3, adding into simulated gastric fluid (adding 10g of pepsin into 800mL of deionized water, acidifying with dilute hydrochloric acid to pH 2.5, adding water to a constant volume of 1000 mL), shake culturing at 37 deg.C for 2h, and centrifuging; placing in simulated intestinal fluid with pH adjusted to 8.0 by sodium hydroxide (adding potassium dihydrogen phosphate 6.8g into 500mL deionized water, adding pancreatin 10g into the simulated intestinal fluid to dissolve, adjusting pH to 6.8 with 0.4% sodium hydroxide solution, adding water to a constant volume of 1000 mL), reacting for 12h, and centrifuging; inoculating to agar medium, culturing for 12 hr, and counting viable bacteria. The results are shown in Table 3.
TABLE 3
Figure BDA0002494382070000151
As can be seen from the data in Table 3: the microencapsulated probiotics can stably colonize the gastrointestinal tract with high viable count and better play physiological roles. Wherein, the components in the wall material can obviously influence the tolerance stability of the product, and the retention rate of viable count of the product without two polypeptide components or one of the two polypeptide components is reduced from 79-87% to 49-60% after passing through simulated gastric fluid and simulated intestinal fluid.
The applicant states that the invention is illustrated by the above examples, but the invention is not limited to the above examples, i.e. it is not meant to be dependent upon the above examples to practice the invention. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.

Claims (16)

1. A microencapsulated probiotic bacteria, characterized in that the microencapsulated probiotic bacteria comprise a core material and a wall material; the core material comprises a composite microbial inoculum, prebiotics and skimmed milk powder; the wall material comprises polypeptide, antioxidant and modified cellulose;
the composite microbial inoculum comprises 50-70 parts of bifidobacterium lactis Bb-12, 30-50 parts of lactobacillus plantarum N13, 10-30 parts of lactobacillus plantarum CW006, 30-50 parts of lactobacillus rhamnosus LRa05 and 30-50 parts of lactobacillus rhamnosus HN001 in parts by weight;
the prebiotics comprise fructo-oligosaccharides and galacto-oligosaccharides;
the polypeptide comprises soybean polypeptide and fish collagen peptide;
the weight ratio of the core material to the wall material is 1:2-2:1.
2. A microencapsulated probiotic bacteria as claimed in claim 1 wherein the core material further comprises a binder and/or polydextrose.
3. A microencapsulated probiotic bacteria as claimed in claim 2 wherein the binder comprises any one or a combination of at least two of maltodextrin, soy protein, pea protein, cyclodextrin, gellan gum, acacia gum or corn syrup.
4. A microencapsulated probiotic bacteria as claimed in claim 1 wherein the weight ratio of soy polypeptide to fish collagen peptide is (1-3): 1.
5. A microencapsulated probiotic bacteria as claimed in claim 1 wherein the antioxidant comprises vitamin E and/or rosemary.
6. A microencapsulated probiotic bacteria as claimed in claim 1 wherein the antioxidant comprises a combination of vitamin E and rosemary.
7. A microencapsulated probiotic bacteria as claimed in claim 6 wherein the weight ratio of vitamin E to rosemary is (3-4): 1.
8. A microencapsulated probiotic bacteria as claimed in claim 1 wherein the modified cellulose comprises sodium carboxymethyl cellulose and/or hydroxypropyl methyl cellulose.
9. A microencapsulated probiotic bacteria as claimed in claim 1 wherein the modified cellulose comprises a combination of sodium carboxymethyl cellulose and hydroxypropyl methyl cellulose.
10. A microencapsulated probiotic bacteria as claimed in claim 9 wherein the weight ratio of sodium carboxymethylcellulose to hydroxypropyl methylcellulose is from 1 (4 to 6).
11. A process for the preparation of a microencapsulated probiotic bacteria as claimed in any of claims 1 to 10, characterized in that it comprises:
mixing core material raw materials to prepare a core material, drying and sieving the core material, and embedding the core material by using a wall material solution to obtain the microencapsulated probiotic bacteria.
12. A process for the preparation of a microencapsulated probiotic as claimed in claim 11 wherein the core material is prepared by a centrifugal pelleting process, an extrusion spheronization process or a rocking granulation process.
13. A process for the preparation of microencapsulated probiotic bacteria as claimed in claim 11 wherein the sieving is by a 40-70 mesh sieve.
14. A process for the preparation of a microencapsulated probiotic bacteria as claimed in claim 11 wherein the concentration of the wall material solution is between 60 and 80%.
15. A process for the preparation of a microencapsulated probiotic as claimed in claim 11, wherein the embedding is carried out using a coating machine with an inlet air temperature of 60-70 ℃, an outlet air temperature of 30-35 ℃, a temperature in the coating chamber of 48-50 ℃ and a spray liquid rotation number of 5-10rpm.
16. Use of a microencapsulated probiotic bacteria according to any one of claims 1 to 10 in the preparation of a probiotic food or health product.
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