CN109275785A - A kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method - Google Patents

A kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method Download PDF

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Publication number
CN109275785A
CN109275785A CN201710588884.0A CN201710588884A CN109275785A CN 109275785 A CN109275785 A CN 109275785A CN 201710588884 A CN201710588884 A CN 201710588884A CN 109275785 A CN109275785 A CN 109275785A
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China
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nano montmorillonite
mycotoxin
feed addictive
bacillus subtilis
antibacterial
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CN201710588884.0A
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Inventor
赵迎庆
刘洋
孙标
邹芳平
万建美
唐寅芳
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Shandong Runmu Biological Technology Co Ltd
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Shandong Runmu Biological Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements

Abstract

The invention discloses a kind of antibacterials, absorbing mycotoxin nano montmorillonite feed addictive preparation method, belong to additive for farm animal feed field, product of the present invention is made of following raw material according to weight percent: nano montmorillonite 40-50%, zinc oxide 1-5%, yeast cell wall 4-6%, bacillus subtilis freeze-dried powder 5-8%, vitamin C 4-5%, propionic acid/propionate 10-15%, sodium Diacetate 7-11%.The present invention has stronger ion-exchange capacity and mycotoxin adsorption ability, the loss of nutriment will not be generated simultaneously, simultaneously the present invention can effectively preventing livestock and poultry diarrhea, inhibit mould proliferation, absorption degradation mycotoxin, mitigate mycotoxin growth of animals or poultry is influenced, improves animal and bird intestines colony balance, improve the use of growth of animals or poultry performance, effective reduction antibiotic during livestock and poultry cultivation.

Description

A kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method
Technical field
The present invention relates to feed additive fields, and in particular to a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed Addictive preparation method.
Background technique
During growth of animal, it all can pass through metabolic nutrition substance along with various harmful bacterias, some meetings, have Be then by generate toxin come influence animal body health and normal immunological, to reduce efficiency of feed utilization and growth Energy.
At present feed addictive in the market, generally use zinc oxide, copper sulphate, Peracetic acid, propionic acid, yeast cells Wall, probiotics etc. inhibit the proliferation of bacterium, fungi, and by adding copper in feed addictive, zinc has not only played its antibacterial Effect can also promote growth of animal, add yeast cell wall and probiotics mainly passes through yeast cell wall and probiotics metabolism Product blocks harmful bacteria in intestinal colonisation, while can also promote the proliferation of intestinal beneficial bacterium, improves intestinal flora balance.
Montmorillonite (montmorillonite) also known as montmorillonite or montmorillonite, are a kind of alumino-silicates, mainly at It is divided into octahedra hectorite microparticle, layered arrangement sial of the 2:1 being pumped by oxygen-octahedron and alumina octahedral containing the crystallization water Hydrochlorate mineral have good dispersibility and ion exchangeable, can be with using this feature of montmorillonite heterogeneity distribution of charges For adsorbing the mycotoxin of mould generation.The montmorillonite product usually to circulate in the market, is mostly to have absorption to aflatoxin Effect, it is unobvious to other mycotoxin adsorption effects, exist simultaneously the drawbacks such as absorption amino acid, vitamin.
Summary of the invention
The present invention provides a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method.Main purpose It is to solve the disadvantage that the prior art currently on the market, existing product is overcome to adsorb single mycotoxin, non-antibacterial, absorption nutrition The defects of element, can effectively inhibit harmful bacteria growth, absorbing mycotoxin, not adsorb nutrient.
The technical solution adopted by the invention is as follows:
A kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method, it is characterised in that with weight percent Meter, is made of following components: nano montmorillonite 40-50%, zinc oxide 1-5%, yeast cell wall 4-6%, bacillus subtilis freeze-drying Powder 5-8%, vitamin C 4-5%, propionic acid/propionate 10-15%, sodium Diacetate 7-11%.
Preferably, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method, according to weight Percentages, including following components: nano montmorillonite 48%, zinc oxide 2.5%, yeast cell wall 5%, bacillus subtilis freeze-drying Powder 7%, vitamin C 4.5%, propionic acid/propionate 14%, sodium Diacetate 10%.
A kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method are as follows:
Respectively by nano montmorillonite, zinc oxide, yeast cell wall, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionic acid Salt, sodium Diacetate proportionally by feed addictive mixing unit be uniformly mixed to get.
The process of preparing of the bacillus subtilis freeze-dried powder, is realized by following process means:
1) bacillus subtilis is subjected to fermented and cultured, culture medium prescription (%): corn flour 3, soybean cake powder using fermentation medium 2 (hydrolyzate adds 121 DEG C of hydrolysis 30min of 0.5%NaOH, cooling, filtering), peptone 0.2, KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06, NaCl 0.2, glucose 0.5 is spat Temperature -80 0.03, pH8.0,37 DEG C of constant temperature incubation 48h.
2) after fermentation, fermentation liquid sterile working is fitted into centrifuge, under the conditions of 25 DEG C, 10000 rpm/min from 5 min of the heart discards supernatant liquid, and gained centrifugal sediment is bacterium mud, and bacterium mud is suspended again with 1% peptone water solution becomes Suspension, and bacillus subtilis bacteria concentration is made to be about 109 cfu/mL。
3) it will be atomized in bacillus subtilis bacterium solution prepared by step 2 injection atomizer, air pressure is when atomization 0.72 kgf/cm2, fluid pressure is 0.45 kgf/cm2。
4) liquid droplet after being atomized is used in the collecting tank equipped with appropriate calcium chloride solution (0.55 mol/L), and process is collected In by magnetic stirring apparatus be gently mixed calcium chloride solution harden 20 min, then use highly dense the screen to filtrate, and with distill washing It washs twice, is then transferred in packaging material (with chitosan with example) lactic acid solution (chitosan C 1.0%(w/v) and lactic acid 0.5%(v/ again V)), being gently mixed 10 min is coated with alginate microcapsules surface uniformly.
5) it filters and then the microcapsules for washing the step 4) preparation is done again in -72 DEG C of 6 h of freeze-drying in -75 DEG C Dry 18 h, pressure is set as 5.28 Pa to get bacillus subtilis freeze-dried powder when dry.
The invention patent enhances the exchanging orientation absorption of its interlayer electronics by being modified to high-purity hydrated alumino-silicate Ability, can selective absorption feed and the intracorporal mycotoxin of animal, and be fixed and lose pathogenicity, with large intestine Wriggling excretes, and avoids intestinal mucosa injury.And the nutriments such as vitamin, microelement are not adsorbed.Yeast is thin in invention Cell wall, bacillus subtilis freeze-drying meal component have mycotoxin, harmful bacteria removal, maintenance intestinal health, raising animal immune Good effect.Propionic acid/propionate, sodium Diacetate ingredient have obvious action to inhibition fungus growth proliferation in the present invention.
Remarkable result of the invention is, the present invention can effectively preventing livestock and poultry diarrhea, inhibit mould proliferation, absorption degradation Mycotoxin, mitigation mycotoxin influence growth of animals or poultry, improve animal and bird intestines colony balance, improve growth of animals or poultry performance, have Use of the reduction antibiotic of effect during livestock and poultry cultivation.
Specific embodiment
In order to which the purpose of the present invention, technical solution and advantage is more clearly understood, with reference to embodiments to this hair It is bright to be described further:
Embodiment one, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method, according to weight percent Meter, each component: nano montmorillonite 40%, zinc oxide 5%, yeast cell wall 6%, bacillus subtilis freeze-dried powder 7%, vitamin C 5%, Propionic acid/propionate 15%, sodium Diacetate 11%.
Nano montmorillonite granularity≤the 100nm, can pass fully through 325 meshes, specific surface area >=240m2/g。
The yeast cell wall its mannan content 28%, beta-dextran content 25%, water-soluble protein content >=30%.
1, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method include the next steps:
1) bacillus subtilis is subjected to fermented and cultured, culture medium prescription (%): corn flour 3, soybean cake powder using fermentation medium 2 (hydrolyzate adds 121 DEG C of hydrolysis 30min of 0.5%NaOH, cooling, filtering), peptone 0.2, KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06, NaCl 0.2, glucose 0.5 is spat Temperature -80 0.03, pH8.0,37 DEG C of constant temperature incubation 48h.
2) after fermentation, fermentation liquid sterile working is fitted into centrifuge, under the conditions of 25 DEG C, 10000 rpm/min from 5 min of the heart discards supernatant liquid, and gained centrifugal sediment is bacterium mud, and bacterium mud is suspended again with 1% peptone water solution becomes Suspension, and bacillus subtilis bacteria concentration is made to be about 109 cfu/mL。
3) it will be atomized in bacillus subtilis bacterium solution prepared by step 2 injection atomizer, air pressure is when atomization 0.72 kgf/cm2, fluid pressure is 0.45 kgf/cm2。
4) liquid droplet after being atomized is used in the collecting tank equipped with appropriate calcium chloride solution (0.55 mol/L), and process is collected In by magnetic stirring apparatus be gently mixed calcium chloride solution harden 20 min, then use highly dense the screen to filtrate, and with distill washing It washs twice, is then transferred in packaging material (with chitosan with example) lactic acid solution (chitosan C 1.0%(w/v) and lactic acid 0.5%(v/ again V)), being gently mixed 10 min is coated with alginate microcapsules surface uniformly.
5) it filters and then the microcapsules for washing the step 4) preparation is done again in -72 DEG C of 6 h of freeze-drying in -75 DEG C Dry 18 h, pressure is set as 5.28 Pa to get bacillus subtilis freeze-dried powder when dry.
2, by nano montmorillonite, zinc oxide, yeast cell wall, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionic acid Salt, sodium Diacetate are proportionally uniformly mixed by feed addictive mixing unit to get feed addictive of the present invention.
Embodiment two, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method, according to weight hundred Divide than meter, each component: nano montmorillonite 44%, zinc oxide 2.5%, yeast cell wall 5%, bacillus subtilis freeze-dried powder 5%, dimension life Plain C4.5%, propionic acid/propionate 13%, sodium Diacetate 9%.
Nano montmorillonite granularity≤the 100nm, can pass fully through 325 meshes, specific surface area >=240m2/g。
The yeast cell wall its mannan content 28%, beta-dextran content 25%, water-soluble protein content >=30%.
1, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method the following steps are included:
1) bacillus subtilis is subjected to fermented and cultured, culture medium prescription (%): corn flour 3, soybean cake powder using fermentation medium 2 (hydrolyzate adds 121 DEG C of hydrolysis 30min of 0.5%NaOH, cooling, filtering), peptone 0.2, KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06, NaCl 0.2, glucose 0.5 is spat Temperature -80 0.03, pH8.0,37 DEG C of constant temperature incubation 48h.
2) after fermentation, fermentation liquid sterile working is fitted into centrifuge, under the conditions of 25 DEG C, 10000 rpm/min from 5 min of the heart discards supernatant liquid, and gained centrifugal sediment is bacterium mud, and bacterium mud is suspended again with 1% peptone water solution becomes Suspension, and bacillus subtilis bacteria concentration is made to be about 109 cfu/mL。
3) it will be atomized in bacillus subtilis bacterium solution prepared by step 2 injection atomizer, air pressure is when atomization 0.72 kgf/cm2, fluid pressure is 0.45 kgf/cm2。
4) liquid droplet after being atomized is used in the collecting tank equipped with appropriate calcium chloride solution (0.55 mol/L), and process is collected In by magnetic stirring apparatus be gently mixed calcium chloride solution harden 20 min, then use highly dense the screen to filtrate, and with distill washing It washs twice, is then transferred in packaging material (with chitosan with example) lactic acid solution (chitosan C 1.0%(w/v) and lactic acid 0.5%(v/ again V)), being gently mixed 10 min is coated with alginate microcapsules surface uniformly.
5) it filters and then the microcapsules for washing the step 4) preparation is done again in -72 DEG C of 6 h of freeze-drying in -75 DEG C Dry 18 h, pressure is set as 5.28 Pa to get bacillus subtilis freeze-dried powder when dry.
2, by nano montmorillonite, zinc oxide, yeast cell wall, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionic acid Salt, sodium Diacetate are proportionally uniformly mixed by feed addictive mixing unit to get feed addictive of the present invention.
Embodiment three, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method, according to weight hundred Divide than meter, each component: nano montmorillonite 48%, zinc oxide 2.5%, yeast cell wall 5%, bacillus subtilis freeze-dried powder 7%, dimension life Plain C4.5%, propionic acid/propionate 14%, sodium Diacetate 10%.
Nano montmorillonite granularity≤the 100nm, can pass fully through 325 meshes, specific surface area >=240m2/g。
The yeast cell wall its mannan content 28%, beta-dextran content 25%, water-soluble protein content >=30%.
1, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method the following steps are included:
1) bacillus subtilis is subjected to fermented and cultured, culture medium prescription (%): corn flour 3, soybean cake powder using fermentation medium 2 (hydrolyzate adds 121 DEG C of hydrolysis 30min of 0.5%NaOH, cooling, filtering), peptone 0.2, KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06, NaCl 0.2, glucose 0.5 is spat Temperature -80 0.03, pH8.0,37 DEG C of constant temperature incubation 48h.
2) after fermentation, fermentation liquid sterile working is fitted into centrifuge, under the conditions of 25 DEG C, 10000 rpm/min from 5 min of the heart discards supernatant liquid, and gained centrifugal sediment is bacterium mud, and bacterium mud is suspended again with 1% peptone water solution becomes Suspension, and bacillus subtilis bacteria concentration is made to be about 109 cfu/mL。
3) it will be atomized in bacillus subtilis bacterium solution prepared by step 2 injection atomizer, air pressure is when atomization 0.72 kgf/cm2, fluid pressure is 0.45 kgf/cm2。
4) liquid droplet after being atomized is used in the collecting tank equipped with appropriate calcium chloride solution (0.55 mol/L), and process is collected In by magnetic stirring apparatus be gently mixed calcium chloride solution harden 20 min, then use highly dense the screen to filtrate, and with distill washing It washs twice, is then transferred in packaging material (with chitosan with example) lactic acid solution (chitosan C 1.0%(w/v) and lactic acid 0.5%(v/ again V)), being gently mixed 10 min is coated with alginate microcapsules surface uniformly.
5) it filters and then the microcapsules for washing the step 4) preparation is done again in -72 DEG C of 6 h of freeze-drying in -75 DEG C Dry 18 h, pressure is set as 5.28 Pa to get bacillus subtilis freeze-dried powder when dry.
2, by nano montmorillonite, zinc oxide, yeast cell wall, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionic acid Salt, sodium Diacetate are proportionally uniformly mixed by feed addictive mixing unit to get feed addictive of the present invention.
Example IV, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method, according to weight hundred Divide than meter, each component: nano montmorillonite 50%, zinc oxide 1%, yeast cell wall 4%, bacillus subtilis freeze-dried powder 8%, vitamin C4%, propionic acid/propionate 10%, sodium Diacetate 7%.
Nano montmorillonite granularity≤the 100nm, can pass fully through 325 meshes, specific surface area >=240m2/g。
The yeast cell wall its mannan content 28%, beta-dextran content 25%, water-soluble protein content >=30%.
1, a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method include the next steps:
1) bacillus subtilis is subjected to fermented and cultured, culture medium prescription (%): corn flour 3, soybean cake powder using fermentation medium 2 (hydrolyzate adds 121 DEG C of hydrolysis 30min of 0.5%NaOH, cooling, filtering), peptone 0.2, KH2PO4 0.15, K2HPO40.15、NaSO4 0.15、CaCl20.03, MnSO4 0.01、MgSO40.06, NaCl 0.2, glucose 0.5 is spat Temperature -80 0.03, pH8.0,37 DEG C of constant temperature incubation 48h.
2) after fermentation, fermentation liquid sterile working is fitted into centrifuge, under the conditions of 25 DEG C, 10000 rpm/min from 5 min of the heart discards supernatant liquid, and gained centrifugal sediment is bacterium mud, and bacterium mud is suspended again with 1% peptone water solution becomes Suspension, and bacillus subtilis bacteria concentration is made to be about 109 cfu/mL。
3) it will be atomized in bacillus subtilis bacterium solution prepared by step 2 injection atomizer, air pressure is when atomization 0.72 kgf/cm2, fluid pressure is 0.45 kgf/cm2。
4) liquid droplet after being atomized is used in the collecting tank equipped with appropriate calcium chloride solution (0.55 mol/L), and process is collected In by magnetic stirring apparatus be gently mixed calcium chloride solution harden 20 min, then use highly dense the screen to filtrate, and with distill washing It washs twice, is then transferred in packaging material (with chitosan with example) lactic acid solution (chitosan C 1.0%(w/v) and lactic acid 0.5%(v/ again V)), being gently mixed 10 min is coated with alginate microcapsules surface uniformly.
5) it filters and then the microcapsules for washing the step 4) preparation is done again in -72 DEG C of 6 h of freeze-drying in -75 DEG C Dry 18 h, pressure is set as 5.28 Pa to get bacillus subtilis freeze-dried powder when dry.
2, by nano montmorillonite, zinc oxide, yeast cell wall, bacillus subtilis freeze-dried powder, vitamin C, propionic acid/propionic acid Salt, sodium Diacetate are proportionally uniformly mixed by feed addictive mixing unit to get feed addictive of the present invention.
Specific experiment verifying is as follows:
Experimental example 1, nutrition adsorption experiment
It takes 3 formulation product of the embodiment of the present invention to be tested, takes 10 100mL triangular flasks, number 1-10 accurately weighs experiment sample Each 1g of product, is added in the triangular flask of number, is dissolved respectively with 10mL Wahaha Pure Water, respectively into the triangular flask of number 1-5 0.01g VB1, VB2, VB6, VB12, methionine is added, is respectively added in 6-10 control group triangular flask general with equivalent of the present invention Logical montmorillonite sample, 10mL Wahaha Pure Water dissolution, sequentially adds 0.01g VB1, VB2, VB6, VB12, methionine, constant temperature 25 DEG C, 100r/min processing 3h, measure VB1, VB2, VB6, VB12, methionine content variation respectively, and test result is shown in Table 1.
1 present invention of table adsorbs situation to each vitamin and methionine
Nutrition type Product of the present invention (g) Common montmorillonite (g)
VB1 0.0098 0.0045
VB2 0.0099 0.0058
VB6 0.0095 0.0061
VB12 0.0097 0.0032
Methionine 0.009 0.0024
The present invention is seldom to vitamin, amino acid adsorbed it can be seen from 1 result of table, does not adsorb substantially, produces using the present invention Product, which will not be led to the problem of, adsorbs nutrient.
It is experimental example 2, mould proof and take off mould effect experiment
3 formulation product of the embodiment of the present invention is taken to be tested, mould proof experiment product of the present invention is public according to 500g/T additive amount and 50 Jin feedstuff mixes, and control group does not add this project product then, keeps 25 DEG C of MW temperature, humidity 60% to store 1 month, inspection Each experimental products peculiar smell, temperature, microorganism situation are surveyed, test result is shown in Table 2.
2 product of the present invention of table tests feedstuff anti-mold effect
Detection Experimental group Control group
Peculiar smell Nothing There is musty
Temperature 25℃-30℃ 30℃-45℃
Aspergillus flavus It is not detected 0.5×104cfu/g
Sickle-like bacteria It is not detected 1×106cfu/g
Escherichia coli 1×103cfu/g 2.01×106cfu/g
Clostridium botulinum It is not detected 1×104cfu/g
Product of the present invention can effectively prevent feedstuff and go mouldy it can be seen from 2 test result of table, to aspergillus flavus, Sickle-like bacteria, clostridium botulinum, Escherichia coli proliferation have apparent inhibitory effect, it is seen that the present invention it is mould proof to feedstuff have very Good function and effect.
It takes 3 formulation product of the embodiment of the present invention to be tested, is added and contains aflatoxin 100ppb, corn equipped with 100g Zeranol 500ppb, vomitoxin 1000ppb feed in, after mixing be packed into 250mL triangular flask, be added 150mL sterile water Dissolution, 25 DEG C of constant temperature, 100r/min handle 2.5h, measure aflatoxin, zearalenone, vomitoxin content respectively, Test result is shown in Table 3:
3 product of the present invention of table tests feed primary toxins removal effect
Detection Before experiment After test Mycotoxin removal rate
Aflatoxin 100ppb 1ppb 99%
Zearalenone 500ppb 101.96ppb 79.6%
Vomitoxin 1000ppb 245.69ppb 75.43%
Product of the present invention has aflatoxin, zearalenone, vomitoxin de- it can be seen from 3 test result of table Toxic effect fruit is aflatoxin 99%, zearalenone 79.6%, vomitoxin 75.43% to the removal rate of mycotoxin, It can be seen that product of the present invention has good function and effect to mycotoxin removal.
The specific embodiment of the invention is described in detail in the above experiment, but the invention is not limited to embodiment, therefore all Technological development personnel are modified and are belonged to the technical solution, the technical matters that obtain in present inventive concept technical foundation in technical field This technology invention protection scope.

Claims (4)

1. a kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method, which is characterized in that with weight percent Than meter, be made of following components: nano montmorillonite 40-50%, zinc oxide 1-5%, yeast cell wall 4-6%, bacillus subtilis are frozen Dry powder 5-8%, vitamin C 4-5%, propionic acid/propionate 10-15%, sodium Diacetate 7-11%.
2. a kind of antibacterial according to claim 1, absorbing mycotoxin nano montmorillonite feed addictive preparation method, Be characterized in that, in percentage by weight, including following components: nano montmorillonite 48%, zinc oxide 2.5%, yeast cell wall 5%, Bacillus subtilis freeze-dried powder 7%, vitamin C 4.5%, propionic acid/propionate 14%, sodium Diacetate 10%.
3. a kind of antibacterial according to claim 1 to 2, absorbing mycotoxin nano montmorillonite feed addictive preparation method, It is characterized in that, the nano montmorillonite granularity≤100nm, passes fully through 325 meshes, specific surface area >=240m2/g。
4. a kind of antibacterial according to claim 1 to 2, absorbing mycotoxin nano montmorillonite feed addictive preparation method, It is characterized in that, the yeast cell wall its mannan content 28%, beta-dextran content 25%, water-soluble protein content >=30%.
CN201710588884.0A 2017-07-19 2017-07-19 A kind of antibacterial, absorbing mycotoxin nano montmorillonite feed addictive preparation method Pending CN109275785A (en)

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CN113558171A (en) * 2020-04-28 2021-10-29 浙江启润生物科技有限公司 Preparation method of antibacterial mycotoxin adsorbing nano montmorillonite feed additive

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