CN105661038A - Antibacterial growth-promoting natural essential oil feed additive and preparation method thereof - Google Patents
Antibacterial growth-promoting natural essential oil feed additive and preparation method thereof Download PDFInfo
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- CN105661038A CN105661038A CN201610044848.3A CN201610044848A CN105661038A CN 105661038 A CN105661038 A CN 105661038A CN 201610044848 A CN201610044848 A CN 201610044848A CN 105661038 A CN105661038 A CN 105661038A
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Abstract
The invention provides an antibacterial growth-promoting natural essential oil feed additive and a preparation method thereof.The antibacterial growth-promoting natural essential oil feed additive comprises, by weight, 10-30% of oregano oil, 1-2% of sodium citrate, 1-2% of lysine, 1-2% of glycine and 15-45% of glucose.The antibacterial growth-promoting natural essential oil feed additive and the preparation method thereof have the advantages that by the aid of broad-spectrum antibacterial effect on escherichia coli and salmonella, livestock and poultry survival rate can be increased remarkably, feed-weight ratio is decreased, livestock and poultry growth is promoted and livestock and poultry health conditions are improved.
Description
Technical field
The present invention relates to the natural essential oil fodder additives of a kind of antibacterial growth-promoting length and its preparation method.
Background technology
Microbiotic, always as fodder additives, is widely used in global range, but a large amount of abuses of antibiotics has caused the problem such as animal derived food antibiotic remains and Resistant strain generation. At present, part country clearly forbids that application microbiotic is as fodder additives, and in 1998, country of Denmark just started to forbid to add in feed microbiotic; European Union has issued in 2006 and has forbidden adding growth-promoting class microbiotic regulation in feed; And FDA (Food and Drug Adminstration) (FDA) just has implemented to forbid the way to accelerate to add in animal rearing process for the purpose of growth of animal microbiotic in December, 2013.
Along with the arriving in the whole world empty microbiotic epoch, noresidue, the safe green fodder additives not producing resistance, by becoming the development trend of whole world feedstuff industry, are also meet people's needs that animal derived food quality is growing gradually. In recent years, under the prerequisite ensureing health and safety, China just progressively Application and Development nontoxic, harmless, pollution-free, have no drug resistance and the green natural fodder additives of noresidue, mainly comprise: bioactive peptide, zymin, probiotics and plants essential oil etc. Wherein producing with plants essential oil series products and practice is being studied at most.
Plants essential oil is the core of plant milk extract, is the mixture that a class has aromaticity volatilization compounds, and plant material is often distinguished according to its fragrance characters. Plants essential oil class fodder additives is the fodder additives of a kind of " safe, stable, efficient and controlled ", in the animal food production of safety, environmental protection and green and occupy an leading position in feed manufacturing process. In plants essential oil, the most representative is Herba Origani Vulgaris quintessence oil, and it extracts from natural phant wild marjoram, has a kind of essence oil of unique antibacterial mechanism and good antioxidant effect. The unique perfume of Herba Origani Vulgaris quintessence oil and activeconstituents can effectively stimulate the digestion organs susceptor of body, strengthen the activity of digestive ferment, promote digesting and assimilating of feed, it is to increase feed conversion rate. In addition, the fragrant phenol of wild marjoram can not make bacterium produce resistance, without any drug residue, is a kind of desirable anti-bacteria agent for feeding. But, common plants essential oil unstable properties, easily volatilizees, oxidizable, and these factors restrict its application in animal productiong.The fragrant phenol of efficient green additive product wild marjoram, by technological means, adopts microcapsule coating technique to carry out bag quilt in fragrant for wild marjoram phenol, reduce its volatility and easily oxidizable, increase the stability of composition, it is widely used in livestock and poultry cultivation at present, and obtains extraordinary result of use.
Summary of the invention
Based on this, the present invention provides the natural essential oil fodder additives of a kind of antibacterial growth-promoting length. By weight percentage, following component is comprised:
Preferably, described carrier is at least one in Macrogol 4000, polyethylene glycol 6000, PEG20000, glycerin fatty acid ester.
The present invention also provides the preparation method of the natural essential oil fodder additives of a kind of antibacterial growth-promoting length, comprises the following steps, adds carrier in material-compound tank, constantly stir, heating, until transparent clear state, adds the fragrant phenol of wild marjoram and mixes, it is atomized with centrifugal atomizer, the particle of gained is added in Methionin, glycine, glucose, mix in mixing machine, inspection, packing, the natural essential oil fodder additives of antibacterial growth-promoting length.
Preferably, Methionin, glycine, glucose powder are broken into 40 orders to 120 orders.
Preferably, the weight ratio added in feed is that every ton of feed adds 1.5 kilogram to 3.5 kilograms.
The present invention has following useful effect:
1, adding wild marjoram oil in weanling pig daily ration, test-results shows, the fragrant phenol of wild marjoram can effectively prevent and treat gastrointestinal tract infection, the quality that increases day by day of piglet can be improved, it is to increase efficiency of feed utilization simultaneously, reduce by thin microbial diarrhoea incidence, improve the speed of growth of piglet, reduce mortality ratio.
2, adding the fragrant phenol of wild marjoram in layer diets, it is possible to lactobacillus number and bifidus bacillus number in enteron aisle are increased, coliform count and salmonella number reduce, and difference is extremely remarkable, it is possible to promote laying hen enteron aisle balance.
What 3, broiler chicken was increased day by day quality and feed-weight ratio by the fragrant phenol of wild marjoram affects the activity that the fragrant phenol of wild marjoram can improve animal intestinal digestive ferment, thus improves feed conversion rate.
Embodiment
Below the preferably embodiment of the present invention is described in further detail:
Embodiment 1
The each composition weight proportioning of the present embodiment composite feed additive:
Carrier adopts Macrogol 4000.
Preparation method is as follows:
Carrier is added in material-compound tank, constantly stir, heating, until transparent clear state, adds the fragrant phenol of wild marjoram and mixes, it is atomized with centrifugal atomizer, the particle of gained is added in Methionin, glycine, glucose, mix in mixing machine, inspection, packing, the natural essential oil fodder additives of antibacterial growth-promoting length.
Embodiment 2
The each composition weight proportioning of the present embodiment composite feed additive:
Carrier adopts polyethylene glycol 6000.
Preparation method is with embodiment 1
Embodiment 3
The each composition weight proportioning of the present embodiment composite feed additive:
Carrier adopts PEG20000.
Preparation method is with embodiment 1
The quality evalution of embodiment 4, wild marjoram oil pre-mixture
The composite feed additive that Example 1-3 is obtained and commercially available wild marjoram oil pre-mixture, carry out the detection of wild marjoram oil stable content (to heat 24 hours under sample is put temperature 35 DEG C ± 1 DEG C condition, with the wild marjoram oil content in pre-mixture after gas chromatography determination heating, to indicate, percentage composition represents content, sign percentage composition before heating is all set as 100%, rate of loss=(the initial wild marjoram oil content-wild marjoram oil percentage composition) of the fragrant phenol of wild marjoram/initial wild marjoram oil content.Detected result is in table 1:
Table 1
Can obtaining by table 1, under asking condition when equal Heating temperature and heating, the heat loss amount of the micro-capsule of wild marjoram oil is far smaller than common wild marjoram oil pulvis.
Embodiment 4
It is added in daily ration to feed 0 healthy aa broiler chicken by embodiment 1, embodiment 2, the obtained fodder additives of embodiment 3, every ton of feed adds natural essential oil fodder additives consumption 2.5kg, and control group only gives daily ration, 20 aa broiler chicken 8000, being divided into 4 groups at random, test-results is as follows.
The fragrant phenol of table 2 wild marjoram is on the impact of broiler chicken surviving rate
| Sample | Age on the 20th | Age on the 25th | Age on the 30th | Age on the 35th | Age on the 40th |
| Embodiment 1 | 98.4% | 98.2% | 98.0% | 97.0% | 96.1% |
| Embodiment 2 | 98.3% | 98.1% | 97.9% | 96.9% | 96.0% |
| Embodiment 3 | 98.5% | 98.2% | 98.1% | 97.1% | 95.8% |
| Control group | 98.7% | 97.6% | 97.2% | 95.2% | 92.0% |
Known according to table 2, from age on the 20th, test group started to feed and adds the fragrant phenol daily ration of wild marjoram, and test group broiler chicken surviving rate is gradually higher than control group, from age on the 35th to 40, test group chick surviving rate was significantly higher than control group (P < 0.05)
Embodiment 5
Containing the fragrant phenol fodder additives of wild marjoram on the impact of growth of meat chicken performance, be added in daily ration to feed 1 healthy aa broiler chicken by embodiment 1, embodiment 2, the obtained fodder additives of embodiment 3, test grouping and process specific as follows shown in.
1st group: embodiment 1 sample, mixed feeding, the weight ratio added in feed is that every ton of feed adds 1.5 kilogram, is used in conjunction 7 days.
2nd group: embodiment 2 sample, mixed feeding, the weight ratio added in feed is that every ton of feed adds 2.5 kilogram, is used in conjunction 7 days.
3rd group: embodiment 3 sample, mixed feeding, the weight ratio added in feed is that every ton of feed adds 3.5 kilogram, is used in conjunction 7 days.
Control group: be negative control group, do not add any fodder additives.
Test-results sees the following form shown in 3:
Table 3
As seen from Table 3: the fragrant phenol of wild marjoram can improve broiler chicken and increase day by day quality and significant difference (P < 0.05); Improve broiler chicken and only deliver quality 143g/ for sale, and significant difference (P < 0.05) significantly improves surviving rate (P < 0.05); Reduce feed-weight ratio, and significant difference (P < 0.05)
Embodiment 6 Example 5 each groups carries out the short growth experiment of piglet
1. for examination animal: age on the 21st weanling pig 200, be divided into 4 treatment group at random, each process 5 repetition, and control group, only to feed, does not add fodder additives, the test group (interpolation 1.5kg/T feed) of the fodder additives prepared by embodiment 1. The test group (adding 2.5kg/T feed) of the fodder additives prepared by embodiment 2, the test group (adding 2.5kg/T feed) of the fodder additives prepared by embodiment 3.
2. trial period is 42 days.
3. testing index: day weight gain; Feedstuff-meat ratio; Diarrhea rate; Death rate.
4. test as shown in table 4
Table 4
Note: numeric representation is mean number ± standard deviation. Different lowercase alphabet shows significant difference (P<0.05), and identical lowercase alphabet differential is different not significantly (P>0.05)
As known from Table 4, it may also be useful to feeding weanling pig containing the fragrant phenol fodder additives of wild marjoram, gaining effect is obvious, and day weight gain improves 3.71%, feed-weight ratio decline 3.85%, diarrhea rate decline 38.02%, death rate decline 42.12%.
Embodiment 7 investigates the fodder additives of antibacterial growth-promoting length to the impact of broiler chicken enteric microorganism bacterium group
Each group of Example 5 is when 30d, and control group and test group, respectively randomly draw 10 chickens, and 3% lysol is slaughtered lethal in disconnected neck mode after sterilizing.Bechtop is got jejunum near x film position content, get 0.50g after fully mixed even and join in the test tube filling 4.5ml work diluent, be 10 at microoscillator vibration this liquid of lOmin-1, times diluent, centrifugal rear Aspirate supernatant 0.5ml adds in another test tube filling 4.5ml diluent and carries out 10-2Doubly dilution, is diluted to 10 successively-6Doubly. Result is as shown in table 3. Inoculation and cultivation lactobacillus: respectively by jejunum and cecal content 10-3-10-6Times diluent 10 μ l is inoculated on lactobacillus selective medium (LBS) ware, and each extent of dilution 3 repetition, to get its mean number. 37 DEG C of sky oxygen cultivate 48h bacterial number the method for plate culture count, represent (1gCFU) with the logarithm of bacterium number in 1g enteron aisle road content. Bifidus bacillus: bifidobacterium culture medium (BS), 37 DEG C of sky oxygen cultivate 48 ear intestinal bacteria: the U.S. blue substratum (} in Yihong } } 37 DEG C have oxygen to cultivate 24h, other working method is the same.
The fodder additives of the antibacterial growth-promoting length of table 5 is on the impact (IgCFU/g) of intestinal microflora
| Sample | Intestinal bacteria | Milk-acid bacteria | Bifidus bacillus |
| Embodiment 1 | 6.34±0.12 | 7.31±0.10 | 7.39±0.15 |
| Embodiment 2 | 6.44±0.12 | 7.78±0.12 | 7.51±0.12 |
| Embodiment 3 | 6.44±0.12 | 7.83±0.51 | 7.63±0.18 |
| Control drug | 6.71±0.81 | 6.25±0.19 | 7.05±0.21 |
Results and analysis: as known from Table 5, test group compares with control group, the fodder additives of antibacterial growth-promoting length feed group jejunum in lactobacillus test group improve than control group: intestinal bacteria and bifidus bacillus number change significant difference: bifidobacterium assays group is higher than control group.
Claims (5)
1. the natural essential oil fodder additives of an antibacterial growth-promoting length, it is characterised in that, by weight percentage, comprise following component:
The fragrant phenol 10-30% of wild marjoram,
Carrier 60%-80%,
Methionin 1%-3%,
Glycine 1%-3%,
Glucose 5%-10%.
2. the natural essential oil fodder additives of an antibacterial growth-promoting length, it is characterised in that, described carrier adopts at least one in Macrogol 4000, polyethylene glycol 6000, PEG20000, glycerin fatty acid ester.
3. prepare the method for the natural essential oil fodder additives of antibacterial growth-promoting length as claimed in claim 1 for one kind, it is characterised in that, comprise following step: in stirred pot, add carrier, constantly stir, heating, until transparent clear state, adds the fragrant phenol of wild marjoram and mixes, it is atomized with centrifugal atomizer, the particle of gained is added in Methionin, glycine, glucose, mix in mixing machine, inspection, packing, namely generates the natural essential oil fodder additives of antibacterial growth-promoting length.
4. prepare the natural essential oil feed addictive preparation method of antibacterial growth-promoting length as claimed in claim 3 for one kind, it is characterised in that, Methionin, glycine, glucose powder are broken into 40 orders to 120 orders.
5. the application of the natural essential oil fodder additives of an antibacterial growth-promoting length as claimed in claim 1, it is characterised in that, the weight ratio added in feed is that every ton of feed adds 1.5 kilogram to 3.5 kilograms.
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| CN106578388A (en) * | 2016-12-23 | 2017-04-26 | 阳江双胞胎饲料有限公司 | Feed additive capable of enhancing piglet immunity |
| CN107156489A (en) * | 2017-06-21 | 2017-09-15 | 福建中农牧生物药业有限公司 | A kind of plants essential oil preparation for improving animal growth rate and preparation method thereof |
| CN111406835A (en) * | 2020-03-13 | 2020-07-14 | 佛山科学技术学院 | Feed for improving growth performance and diarrhea of weaned piglets |
| CN114886034A (en) * | 2022-05-30 | 2022-08-12 | 华兴宠物食品有限公司 | Dog food for improving intestinal flora balance and preparation method thereof |
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| CN114886034A (en) * | 2022-05-30 | 2022-08-12 | 华兴宠物食品有限公司 | Dog food for improving intestinal flora balance and preparation method thereof |
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