CN105994954A - Microencapsulated microecological preparation and preparation method thereof - Google Patents
Microencapsulated microecological preparation and preparation method thereof Download PDFInfo
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- CN105994954A CN105994954A CN201610317878.7A CN201610317878A CN105994954A CN 105994954 A CN105994954 A CN 105994954A CN 201610317878 A CN201610317878 A CN 201610317878A CN 105994954 A CN105994954 A CN 105994954A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 16
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 claims abstract description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 11
- JLFNLZLINWHATN-UHFFFAOYSA-N pentaethylene glycol Chemical compound OCCOCCOCCOCCOCCO JLFNLZLINWHATN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 9
- 239000008158 vegetable oil Substances 0.000 claims abstract description 9
- 239000004310 lactic acid Substances 0.000 claims abstract description 8
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 8
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims abstract description 7
- 229940016286 microcrystalline cellulose Drugs 0.000 claims abstract description 7
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims abstract description 7
- 239000008108 microcrystalline cellulose Substances 0.000 claims abstract description 7
- 229920002261 Corn starch Polymers 0.000 claims abstract description 5
- 239000008120 corn starch Substances 0.000 claims abstract description 5
- 239000003960 organic solvent Substances 0.000 claims abstract description 3
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract 4
- 239000003795 chemical substances by application Substances 0.000 claims description 45
- 230000000813 microbial effect Effects 0.000 claims description 43
- 239000002245 particle Substances 0.000 claims description 14
- 239000004005 microsphere Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 238000001291 vacuum drying Methods 0.000 claims description 6
- 235000019482 Palm oil Nutrition 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 5
- 238000000576 coating method Methods 0.000 claims description 5
- 239000002540 palm oil Substances 0.000 claims description 5
- 241000186660 Lactobacillus Species 0.000 claims description 4
- 238000000498 ball milling Methods 0.000 claims description 4
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 4
- 229940039696 lactobacillus Drugs 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 241000195474 Sargassum Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 abstract description 16
- 235000010413 sodium alginate Nutrition 0.000 abstract description 16
- 239000000661 sodium alginate Substances 0.000 abstract description 16
- 229940005550 sodium alginate Drugs 0.000 abstract description 16
- 230000009286 beneficial effect Effects 0.000 abstract description 15
- 241000894006 Bacteria Species 0.000 abstract description 14
- 244000005700 microbiome Species 0.000 abstract description 8
- 150000001875 compounds Chemical class 0.000 abstract description 4
- 210000002784 stomach Anatomy 0.000 abstract description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 2
- 239000001116 FEMA 4028 Substances 0.000 abstract 3
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 abstract 3
- 235000011175 beta-cyclodextrine Nutrition 0.000 abstract 3
- 229960004853 betadex Drugs 0.000 abstract 3
- 238000000034 method Methods 0.000 description 17
- 230000000968 intestinal effect Effects 0.000 description 12
- 230000000694 effects Effects 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 8
- 238000004090 dissolution Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 230000014759 maintenance of location Effects 0.000 description 6
- 239000006041 probiotic Substances 0.000 description 5
- 235000018291 probiotics Nutrition 0.000 description 5
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 210000004051 gastric juice Anatomy 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000003094 microcapsule Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000000529 probiotic effect Effects 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000007922 dissolution test Methods 0.000 description 2
- 229960004756 ethanol Drugs 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000186016 Bifidobacterium bifidum Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 229950005162 benexate Drugs 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- IAXUQWSLRKIRFR-SAABIXHNSA-N chembl2104696 Chemical compound C1C[C@@H](CNC(=N)N)CC[C@@H]1C(=O)OC1=CC=CC=C1C(=O)OCC1=CC=CC=C1 IAXUQWSLRKIRFR-SAABIXHNSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000000280 densification Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000011978 dissolution method Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 230000004719 natural immunity Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
Landscapes
- Medicinal Preparation (AREA)
Abstract
The invention discloses a microencapsulated microecological preparation and a preparation method thereof. The microencapsulated microecological preparation is prepared from, by weight, 10<7>-10<11> CFU/part of lactic acid bacteria fermented solution, 20-40 parts of beta-cyclodextrin, 1-5 parts of PEG400, 0.1-5 parts of trehalose, 5-20 parts of microcrystalline cellulose, 0.1-5 parts of sodium alginate, 20-40 parts of corn starch, 5-20 parts of vegetable oil and 15-30 parts of organic solvent. According to the microencapsulated microecological preparation and the preparation method thereof, PEG400, trehalose and beta-cyclodextrin are adopted to prepare an inclusion compound, and beneficial microorganisms are located in a protective film in a molecular state and then aggregated in the inclusion compound which has a compact structure and is formed by beta-cyclodextrin; sodium alginate and the vegetable oil are adopted to conduct double-layer microencapsulation treatment, and the microencapsulated microecological preparation is obtained; the preparation can be released after reaching the intestinal tract, the beneficial microorganisms are prevented from being destroyed by being released in the stomach, and the survival rate of the beneficial microorganisms is increased.
Description
Technical field
The present invention relates to animal feed adding technique field, particularly relate to a kind of microencapsulation microbial ecological agent and preparation side thereof
Method.
Background technology
Animal microencapsulation microbial ecological agent is, microbial state treatment theory, Tiny ecosystem nutrition reason theoretical according to microecological balance
Discuss and in Tiny ecosystem preventing and treating theory selection animal body, microflora member and promotion material thereof are made through special processing process；With
In regulation animal body microecological balance, have and directly to the inhibitory action of intestinal toxic microorganism or passed through by enhancing animal
Enhancing non-specific immunity function prevents disease, thus promotes growth of animal or the class medicine improved food conversion ratio or raise
Feed additives.The biological product being made up of normal flora Institute of Micro-biology due to it, therefore, are also called live bacteria agent or physiology bacterium
Seedling.
Microencapsulation microbial ecological agent has no side effect with it, has no drug resistance, noresidue, low cost, effect significantly, do not pollute
The features such as environment, gradually obtain the approval of vast cultivation circle colleague.The mechanism of action of microencapsulation microbial ecological agent specifically includes that
(1) competitive adhere to: by intestinal bound site inhibition effect, form biological protection film, protection humans and animals from
The infringement of pathogenic microorganism.
(2) antimicrobial effect: the lactic acid that lactic acid bacteria fermentation produces reduces gut pH, stops growth of pathogenic bacteria, produces class thin
Rhzomorph can kill pathogenic bacterium.
(3) immunologic function is activated: microencapsulation microbial ecological agent bacterial strain can affect host's natural immunity, humoral and cellular immune response
System, improves intestinal antibody horizontal and macrophage activity, enhancing human body immunity function.
(4) improve digestion power: microencapsulation microbial ecological agent bacterial strain can stimulate the growth of intestinal villi, increase nutrition and inhale
Receive area, improve food or feed digestion level.
China's Ministry of Agriculture's approval in 1994 uses the kind of microencapsulation microbial ecological agent to have: bacillus cereus, hay bud
Spore bacillus, streptococcus faecalis, bacillus bifidus, lactobacillus, Streptococcus lactis (Lister) Lohnis 1909.554. etc., wherein most falls within lactic acid bacteria class.
Although effect of microencapsulation microbial ecological agent has extensively been proved and has been accepted；But, common viable bacteria body microencapsulation
Microbial ecological agent yet suffers from following problem:
(1) affected, its active ingredient easy in inactivation by environmental condition (temperature, moisture, acid-base value etc.), thus reduced use
Effect.
(2) pyroprocess of the microencapsulation course of processing, feed preparation and pelletize makes substantial amounts of viable bacteria lethal.
(3) in feedstuff add or therapeutic process use antibiotic and chemosynthesis antibacterial, life micro-to microencapsulation
State preparation has killing or inhibiting effect equally.
(4) effect of the environment such as the hydrochloric acid not being highly resistant in human or animal's digestive tract or bile, in the field planting of intestinal
Ability.
Microcapsule technology is a kind of effective ways improving probiotic active, is embedded and in protective structures by probiotic bacteria, energy
Enough strengthen the resistivity of they poor environments to external world, be conducive to making it pass through stomach and arrive intestinal, play it at intestinal
Beneficial effect.Technology at present uses double-deck peplos, can solve the probiotics sensitivity to temperature to a certain extent, but effect is also
Have much room for improvement.
Summary of the invention
The invention provides a kind of microencapsulation microbial ecological agent and preparation method thereof, this microencapsulation microbial ecological agent not only may be used
Improve beneficial microbe stability in the course of processing, moreover it is possible to guarantee its transport, store and feed manufacturing process from height
The destruction of the environment such as temperature, high heat, improves product viable count before application, beneficial microbe can be made to pass through stomach simultaneously
Arrive intestinal, play beneficial effect at intestinal colonisation.
The invention provides a kind of microencapsulation microbial ecological agent, in terms of parts by weight, including following components:
As preferably, described microencapsulation microbial ecological agent, in terms of parts by weight, including following components:
It is further preferred that described microencapsulation microbial ecological agent, in terms of parts by weight, including following components:
Above-mentioned lactobacillus suspension is the bacterium solution of lactic acid bacteria cultivation and fermentation gained, without special handling, is directly used in preparation bag
Compound, can reduce the mortality rate of thalline.
As preferably, described vegetable oil is hydrogenated palm oil.
Preferably, described organic solvent is dehydrated alcohol.
Present invention also offers the preparation method of described microencapsulation microbial ecological agent, including:
(1), after lactobacillus suspension, PEG400 and trehalose being mixed, carry out ball milling with beta-schardinger dextrin-and mix, it is thus achieved that lactic acid bacteria
Benexate Hydrochloride；
(2) described clathrate is after low-temperature vacuum drying, pulverizing, mixes with microcrystalline Cellulose, corn starch, then warp
Sodium alginate aqueous solution spray carries out ball blast pelletize, it is thus achieved that microsphere particle；
(3) described microsphere particle is after low-temperature vacuum drying, carries out end spray coating with vegetable oil, obtains microencapsulation Tiny ecosystem
Preparation.
Normal packet compound only uses beta-schardinger dextrin-, and the inventive method is also added into PEG400 and trehalose, can be at molecularity
Around each probiotic bacteria, protecting film is formed, it is thus achieved that the clathrate of high stability under state.
The making formula of clathrate to beneficial microorganism stability tool play a very important role, use trehalose and
PEG400, can make beneficial microbe be protected under molecularity, and the proportioning of clathrate directly determines the effect of inclusion,
Clathrate composition is too low, and inclusion is incomplete, and clathrate composition is too high, and beneficial microbe content is on the low side, impact application effect.
With clathrate as core, at its outer layer application sodium alginate and vegetable oil, clathrate is carried out at double-layer microcapsule
Reason, it is thus achieved that microencapsulation microbial ecological agent product；Sodium alginate and the Combined Protection of vegetable oil, it is possible to make microencapsulation microbial ecological agent
Discharge after arriving intestinal, it is to avoid beneficial microbe discharges under one's belt and destroyed, and improves beneficial microorganism survival rate.
In conventional method, when using sodium alginate, directly mix with other raw materials, then pelletize；And the inventive method makes
Spray with sodium alginate, utilize sodium alginate film forming characteristics, make sodium alginate disperse evenly, improve the densification of grain structure,
Improve the harsh and unforgiving environments destruction to effective ingredient.
As preferably, in step (1), the rotating speed of ball milling is 150～200 revs/min.Rotating speed is too fast, easily causes logistics
Flying upward, rotating speed is too low, and clathrate inclusion is incomplete, and in following process process and finished product application process, loss of effective components is big.
As preferably, in step (2), the temperature of described low-temperature vacuum drying is 35～40 DEG C；In crushing process, also 35
～pulverize under the conditions of 40 DEG C；Powder after pulverizing crosses 60 mesh sieves.
Preferably, in step (2), described ball blast pelletize use spray liquid be mass fraction be the sodium alginate of 0.2%
Aqueous solution.
The condition of described ball blast pelletize is: rotating speed is 10～20Hz, and atomizing pressure is 0.1～0.2MPa, peristaltic pump flow velocity
It is 20～30r/min.
Preferably, the particle diameter of described microsphere particle is 30 mesh.
In step (3), the temperature of described low-temperature vacuum drying is 35～40 DEG C；Described end spray coating is cyclone fluidized bed
Inside carry out.Wherein, as preferably, the condition of end spray coating is: inlet temperature is 30～40 DEG C, and air outlet is 30～40 DEG C, mist
Changing pressure is 0.1～0.2MPa, and coating liquid flow velocity is 15～25r/min.
Compared with prior art, the method have the advantages that
(1) present invention uses PEG400, trehalose, beta-schardinger dextrin-to make clathrate so that beneficial microbe is first with molecule
State is in protecting film, reassociates in the clathrate of the compact structure of beta-schardinger dextrin-formation；Use sodium alginate again and plant
Thing oil carries out double-layer microcapsule process, it is thus achieved that microencapsulation microbial ecological agent, it is possible to make preparation discharge after arriving intestinal, it is to avoid useful
Microorganism discharges under one's belt and is destroyed, and improves beneficial microorganism survival rate.
(2) clathrate is applied to the processing of microencapsulation microbial ecological agent by the present invention first, uses special core and bag
Material formula, obtains microencapsulation microbial ecological agent by inclusion technique, micropill pelletize and end spray packaging technique, and said preparation is not only avoided
Effective ingredient loss during processing stores transport and feed manufacturing, and can protect effective ingredient, it is prevented effectively from stomach
The destruction of sour and various enzyme and decomposition, arrive intestinal colonisation smoothly and play beneficial effect, efficiently solve probiotics processing and answer
The difficult problem that the utilization rate caused is extremely low is easily inactivated during with.
(3) microencapsulation microbial ecological agent particle size distribution of the present invention is good, mobility, it is easy to commercial application.
(4) microencapsulation microbial ecological agent of the present invention can directly with feedstuff mixing feeding, feed manufacturing process losses is less.
(5) present invention first makes microsphere particle with sodium alginate, carries out the double-deck peplos work of Vegetable oil lipoprotein coating the most again
Skill, is to create in current microencapsulation microbial ecological agent protection technique.
Detailed description of the invention
1, retention rate and the assay method of dissolution rate:
The microencapsulation microbial ecological agent of acquisition is placed under rigor condition (60 DEG C process 24h) preserve, before and after detection processes
The lactic acid bacterium number of microbial ecological agent, calculating retention rate, retention rate=(number of viable/initial number of viable after process) ×
100%.
The microbial ecological agent obtained carries out the mensuration of dissolution, and detection method is:
(1) temperature of regulation dissolution test system is 37 DEG C, and rotating speed is 100r/min, pours artificial preparation in stripping rotor into
Simulated gastric fluid, open machine, after equitemperature is stable, standby；
(2) take 4g Tiny ecosystem sample (being accurate to 0.0002), put into the molten of the middle dissolution test system debugged of step (1)
Go out in cup, after 1h, 2h, 4h, 8h, be sampled detection, calculate dissolution rate；
The computing formula of dissolution rate is: dissolution rate=(dissolution content/initial content) × 100%.
2, bacillus subtilis bacterium solution is from Jiangsu Lv Ke Bioisystech Co., Ltd, and lot number is 20150811；Addicted to yogurt bar
Bacterium solution is from Jiangsu Lv Ke Bioisystech Co., Ltd, and lot number is 20150953.
Embodiment 1
A kind of microencapsulation microbial ecological agent, including following components and consumption:
Bacillus subtilis bacterium solution 108CFU/ part, beta-schardinger dextrin-40 parts, PEG400 3 parts, trehalose 5 parts, microcrystalline Cellulose
10 parts, sodium alginate 1 part, corn starch 30 parts, hydrogenated palm oil 10 parts, dehydrated alcohol 30 parts.
The preparation method of above-mentioned microencapsulation microbial ecological agent is:
(1) the bacillus subtilis bacterium solution, PEG400 and the trehalose that take above-mentioned consumption mix, and carry out ball with beta-schardinger dextrin-
Mill mixing, rotating speed is 200 turns/min, it is thus achieved that clathrate；
(2) described clathrate is dried under conditions of 35 DEG C, pulverizes, cross 60 mesh sieves, then with microcrystalline Cellulose, Semen Maydis
Starch mixes, and then with sodium alginate aqueous solution that mass fraction is 0.2% as spray liquid, carries out ball blast pelletize, controls
Rotating speed 15Hz, atomizing pressure 0.15MPa, peristaltic pump flow speed control, at 30r/min, obtains the microsphere particle of 30 mesh；
(3) described microsphere particle is dried under conditions of 35 DEG C, then with the ethanol solution of hydrogenated palm oil
Carry out end spray coating, inlet temperature 35 DEG C, air-out 38 DEG C, atomizing pressure 0.15MPa, flow velocity 20r/min for coating liquid, obtain micro-
Encapsulated microbial ecological agent.
The retention rate measuring above-mentioned microencapsulation microbial ecological agent is 85.18%, and gastric juice dissolution rate is 9.36%.
Embodiment 2
A kind of microencapsulation microbial ecological agent, including following components and consumption:
Bacillus acidophilus's liquid 107CFU/ part, beta-schardinger dextrin-20 parts, PEG400 5 parts, trehalose 5 parts, microcrystalline Cellulose 10
Part, sodium alginate 5 parts, corn starch 30 parts, hydrogenated palm oil 20 parts, dehydrated alcohol 30 parts.
The preparation method of above-mentioned microencapsulation microbial ecological agent is:
(1) the bacillus acidophilus's liquid, PEG400 and the trehalose that take above-mentioned consumption mix, and carry out ball with beta-schardinger dextrin-
Mill mixing, 150 turns/min of rotating speed, it is thus achieved that clathrate；
(2) described clathrate is dried under conditions of 35 DEG C, pulverizes, and crosses 20 mesh sieves, then microcrystalline Cellulose, Semen Maydis form sediment
Powder mixes, and then with sodium alginate aqueous solution that mass fraction is 0.2% as spray liquid, carries out ball blast pelletize, controls to turn
Speed 15Hz, atomizing pressure 0.15MPa, peristaltic pump flow speed control, at 30r/min, obtains the microsphere particle of 30 mesh；
(3) described microsphere particle is dried under conditions of 35 DEG C, then carry out the end spray coating, inlet temperature 35 DEG C,
Air-out 38 DEG C, atomizing pressure 0.15MPa, with the ethanol solution of castor oil hydrogenated as coating liquid, flow velocity 20r/min, obtain
Microencapsulation microbial ecological agent.
The retention rate measuring above-mentioned microencapsulation microbial ecological agent is 86.25%, and gastric juice dissolution rate is 10.45%.
Embodiment 3～7
In addition to the parameter that below table presents, other guide is same as in Example 1, prepares microencapsulation Tiny ecosystem system
Agent；Particular content and result, as shown in table 1.
The microencapsulation microbial ecological agent that the different preparation method of table 1 obtains retention rate under rigor condition and gastric juice dissolution rate
Claims (10)
1. a microencapsulation microbial ecological agent, it is characterised in that in terms of parts by weight, including following components:
2. microencapsulation microbial ecological agent as claimed in claim 1, it is characterised in that in terms of parts by weight, including following components:
3. microencapsulation microbial ecological agent as claimed in claim 1, it is characterised in that in terms of parts by weight, including following components:
4. microencapsulation microbial ecological agent as claimed in claim 1, it is characterised in that described vegetable oil is hydrogenated palm oil.
5. microencapsulation microbial ecological agent as claimed in claim 1, it is characterised in that described organic solvent is dehydrated alcohol.
6. the preparation method of the microencapsulation microbial ecological agent as described in any one of Claims 1 to 5, it is characterised in that including:
(1), after lactobacillus suspension, PEG400 and trehalose being mixed, carry out ball milling with beta-schardinger dextrin-and mix, it is thus achieved that the β of lactic acid bacteria-
Cyclodextrin clathrate；
(2) described clathrate is after low-temperature vacuum drying, pulverizing, mixes with microcrystalline Cellulose, corn starch, then through Sargassum
Acid sodium aqueous solution spray carries out ball blast pelletize, it is thus achieved that microsphere particle；
(3) described microsphere particle is after low-temperature vacuum drying, carries out end spray coating with vegetable oil, obtains microencapsulation Tiny ecosystem system
Agent.
7. preparation method as claimed in claim 6, it is characterised in that in step (1), the rotating speed of described ball milling is 150～200
Rev/min.
8. preparation method as claimed in claim 6, it is characterised in that the condition of described ball blast pelletize is: rotating speed be 10～
20Hz, atomizing pressure is 0.1～0.2MPa, and peristaltic pump flow velocity is 20～30r/min.
9. preparation method as claimed in claim 6, it is characterised in that the particle diameter of described microsphere particle is 30 mesh.
10. preparation method as claimed in claim 6, it is characterised in that in step (3), the condition of end spray coating is: air intake temperature
Degree is 30～40 DEG C, and air outlet is 30～40 DEG C, and atomizing pressure is 0.1～0.2MPa, and coating liquid flow velocity is 15～25r/min.
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