CN105994954B - A kind of microencapsulation probiotics and preparation method thereof - Google Patents
A kind of microencapsulation probiotics and preparation method thereof Download PDFInfo
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- CN105994954B CN105994954B CN201610317878.7A CN201610317878A CN105994954B CN 105994954 B CN105994954 B CN 105994954B CN 201610317878 A CN201610317878 A CN 201610317878A CN 105994954 B CN105994954 B CN 105994954B
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- 239000006041 probiotic Substances 0.000 title claims abstract description 50
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 150000001875 compounds Chemical class 0.000 claims abstract description 21
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 13
- 239000001116 FEMA 4028 Substances 0.000 claims abstract description 12
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims abstract description 12
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims abstract description 12
- 229960004853 betadex Drugs 0.000 claims abstract description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 11
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 claims abstract description 9
- JLFNLZLINWHATN-UHFFFAOYSA-N pentaethylene glycol Chemical compound OCCOCCOCCOCCOCCO JLFNLZLINWHATN-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 8
- 239000008158 vegetable oil Substances 0.000 claims abstract description 8
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- 239000008120 corn starch Substances 0.000 claims abstract description 6
- 229940099112 cornstarch Drugs 0.000 claims abstract description 6
- 241000186660 Lactobacillus Species 0.000 claims abstract description 4
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- 239000003960 organic solvent Substances 0.000 claims abstract description 3
- 241000894006 Bacteria Species 0.000 claims description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 12
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- 238000000576 coating method Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
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- 238000001291 vacuum drying Methods 0.000 claims description 7
- 239000004310 lactic acid Substances 0.000 claims description 6
- 235000014655 lactic acid Nutrition 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 235000019482 Palm oil Nutrition 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 238000005469 granulation Methods 0.000 claims description 5
- 230000003179 granulation Effects 0.000 claims description 5
- 239000002540 palm oil Substances 0.000 claims description 5
- 238000000498 ball milling Methods 0.000 claims description 4
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- 241001474374 Blennius Species 0.000 claims 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000002274 desiccant Substances 0.000 claims 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 18
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 abstract description 16
- 235000010413 sodium alginate Nutrition 0.000 abstract description 16
- 239000000661 sodium alginate Substances 0.000 abstract description 16
- 229940005550 sodium alginate Drugs 0.000 abstract description 16
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- 244000005700 microbiome Species 0.000 abstract description 6
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- 239000007788 liquid Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 230000014759 maintenance of location Effects 0.000 description 6
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- 238000001514 detection method Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
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- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
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- 238000005516 engineering process Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 229960004756 ethanol Drugs 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 229950005162 benexate Drugs 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- IAXUQWSLRKIRFR-SAABIXHNSA-N chembl2104696 Chemical compound C1C[C@@H](CNC(=N)N)CC[C@@H]1C(=O)OC1=CC=CC=C1C(=O)OCC1=CC=CC=C1 IAXUQWSLRKIRFR-SAABIXHNSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
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- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000000280 densification Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 208000021760 high fever Diseases 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
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- 230000015788 innate immune response Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
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- 230000010534 mechanism of action Effects 0.000 description 1
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- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
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- 239000008188 pellet Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
Abstract
The invention discloses a kind of microencapsulation probiotics and preparation method thereof, the microencapsulation probiotics based on parts by weight, including following components: lactobacillus suspension 107~1011CFU/ parts；20~40 parts of beta-cyclodextrin；1~5 part of PEG400；0.1~5 part of trehalose；5~20 parts of microcrystalline cellulose；0.1~5 part of sodium alginate；20~40 parts of cornstarch；5~20 parts of vegetable oil；15~30 parts of organic solvent.The present invention makes inclusion compound using PEG400, trehalose, beta-cyclodextrin, so that beneficial microbe is first in protective film with molecular state, reassociates in the inclusion compound for the compact structure that beta-cyclodextrin is formed；Double-layer microcapsule processing is carried out using sodium alginate and vegetable oil again, obtains microencapsulation probiotics, is discharged after preparation can be made to reach enteron aisle, is avoided beneficial microbe from discharging and destroyed under one's belt, improve beneficial microorganism survival rate.
Description
Technical field
The present invention relates to animal feed adding technique field more particularly to a kind of microencapsulation probiotics and its preparation sides
Method.
Background technique
Animal microencapsulation probiotics are, microbial state treatment theory, Tiny ecosystem nutrition reason theoretical according to microecological balance
It is made by with microflora member in the theoretical selection animal body of Tiny ecosystem prevention and treatment and its promotion substance through special processing process；With
In adjusting animal body microecological balance, have directly by enhancing animal to the inhibiting effect of intestinal toxic microorganism or passing through
Enhancing non-specific immune function prevents disease, to promote growth of animal or a kind of drug for improving food conversion ratio or feeding
Feed additives.Since it is the biological products made of normal flora Institute of Micro-biology, also known as live bacteria agent or physiology bacterium
Seedling.
Microencapsulation probiotics are had no toxic side effect with it, are had no drug resistance, noresidue, at low cost, significant effect, do not polluted
The features such as environment, gradually obtains the approval of vast cultivation circle colleague.The mechanism of action of microencapsulation probiotics specifically includes that
(1) competitive adherency: by enteron aisle combination steric effect, formation biological protection film, protect humans and animals from
The infringement of pathogenic microorganisms.
(2) antimicrobial effect: the lactic acid that lactobacillus-fermented generates reduces gut pH, prevents growth of pathogenic bacteria, and it is thin to generate class
Rhzomorph can kill pathogenic bacteria.
(3) activate immune function: microencapsulation probiotics bacterial strain can influence host's innate immunity, humoral and cellular immune response
System improves enteron aisle antibody level and macrophage activity, enhances body's immunity.
(4) improve digestion power: microencapsulation probiotics bacterial strain can stimulate the growth of intestinal villi, increase nutrition and inhale
Area is received, food is improved or feed digestion is horizontal.
China's Ministry of Agriculture's approval in 1994 is had using the kind of microencapsulation probiotics: bacillus cereus, withered grass bud
Spore bacillus, streptococcus fecalis, Bifidobacterium, Bacillus acidi lactici, streptococcus lactis etc., wherein most also belongs to lactic acid bacteria class.
Although the effect of microencapsulation probiotics, is proved and is received extensively；But common viable bacteria body microencapsulation
Probiotics still have following problem:
(1) it is influenced by environmental condition (temperature, moisture, pH value etc.), effective ingredient easy in inactivation, to reduce use
Effect.
(2) pyroprocess of microencapsulation process, feed preparation and granulation keeps a large amount of viable bacteria lethal.
(3) being added in feed or in antibiotic and chemical synthesis antibacterial agent that therapeutic process uses, to the micro- life of microencapsulation
State preparation equally has killing or inhibiting effect.
(4) it is not highly resistant to the effect of the environment such as hydrochloric acid or the bile in human or animal's alimentary canal, in the field planting of enteron aisle
Ability is poor.
Microcapsule technology be improve probiotic active a kind of effective ways, by probiotics embedding with protective structures in, energy
Enough enhance their resistivities to extraneous poor environment, being conducive to, which makes it pass through stomach, reaches enteron aisle, plays it in enteron aisle
Beneficial effect.Technology mostly uses the double-deck coating at present, can solve beneficial bacterium to a certain extent to the sensibility of temperature, but effect is also
It is to be improved.
Summary of the invention
The present invention provides a kind of microencapsulation probiotics and preparation method thereof, which not only may be used
Improve the stability of beneficial microbe in process, moreover it is possible to ensure it in transport, storage and feed processing process from height
The destruction of the environment such as temperature, high fever improves the viable count of product before application, while beneficial microbe can be made to pass through stomach
Enteron aisle is reached, plays beneficial effect in intestinal colonisation.
The present invention provides a kind of microencapsulation probiotics, based on parts by weight, including following components:
Preferably, the microencapsulation probiotics, based on parts by weight, including following components:
It is further preferred that the microencapsulation probiotics, based on parts by weight, including following components:
Above-mentioned lactobacillus suspension is the resulting bacterium solution of lactic acid bacteria culture fermentation, without specially treated, is directly used in preparation packet
Object is closed, the death rate of thallus can be reduced.
Preferably, the vegetable oil is hydrogenated palm oil.
Preferably, the organic solvent is dehydrated alcohol.
The present invention also provides the preparation methods of the microencapsulation probiotics, comprising:
(1) after mixing lactobacillus suspension, PEG400 and trehalose, ball milling mixing is carried out with beta-cyclodextrin, obtains lactic acid bacteria
Benexate Hydrochloride；
(2) inclusion compound is mixed after low-temperature vacuum drying, crushing with microcrystalline cellulose, cornstarch, then is passed through
Sodium alginate aqueous solution spray carries out ball blast granulation, obtains microsphere particle；
(3) microsphere particle carries out bottom spray coating with vegetable oil, obtains microencapsulation Tiny ecosystem after low-temperature vacuum drying
Preparation.
Common inclusion compound only uses beta-cyclodextrin, and the method for the present invention is also added into PEG400 and trehalose, can be in molecularity
Protective film is formed around each probiotics under state, obtains the inclusion compound of high stability.
The production formula of inclusion compound plays a very important role to beneficial microorganism stability tool, using trehalose and
PEG400 can be such that beneficial microbe is protected under molecular state, and the proportion of inclusion compound directly determines the effect of inclusion,
Inclusion compound ingredient is too low, and not exclusively, inclusion compound ingredient is excessively high, and beneficial microbe content is relatively low, influences application effect for inclusion.
Using inclusion compound as core material, inclusion compound is carried out at double-layer microcapsule in its outer layer application sodium alginate and vegetable oil
Reason obtains microencapsulation probiotics product；The Combined Protection of sodium alginate and vegetable oil can make microencapsulation probiotics
It is discharged after reaching enteron aisle, avoids beneficial microbe from discharging and destroyed under one's belt, improve beneficial microorganism survival rate.
In conventional method, when using sodium alginate, directly mixes with other raw materials, be then granulated；And the method for the present invention makes
It is sprayed with sodium alginate, using sodium alginate film forming characteristics, makes sodium alginate dispersion more evenly, improve the densification of grain structure,
Improve destruction of the harsh environment to effective component.
Preferably, the revolving speed of ball milling is 150~200 revs/min in step (1).Revolving speed is too fast, easily causes logistics
It flies upward, revolving speed is too low, and not exclusively, loss of effective components is big in following process process and finished product application process for inclusion compound inclusion.
Preferably, the temperature of the low-temperature vacuum drying is 35~40 DEG C in step (2)；In crushing process, also 35
It is crushed under the conditions of~40 DEG C；Smashed powder crosses 60 meshes.
Preferably, in step (2), the ball blast is granulated the sodium alginate that the spray liquid used is 0.2% for mass fraction
Aqueous solution.
The condition that the ball blast is granulated are as follows: revolving speed is 10~20Hz, and atomizing pressure is 0.1~0.2MPa, wriggling flow rate pump
For 20~30r/min.
Preferably, the partial size of the microsphere particle is 30 mesh.
In step (3), the temperature of the low-temperature vacuum drying is 35~40 DEG C；The bottom spray coating is cyclone fluidized bed
Interior progress.Wherein, preferably, the condition of bottom spray coating are as follows: inlet air temperature is 30~40 DEG C, and air outlet is 30~40 DEG C, mist
Change pressure is 0.1~0.2MPa, and coating flow velocity is 15~25r/min.
Compared with prior art, the invention has the following advantages:
(1) present invention makes inclusion compound using PEG400, trehalose, beta-cyclodextrin, so that beneficial microbe is first with molecule
State is in protective film, is reassociated in the inclusion compound for the compact structure that beta-cyclodextrin is formed；Sodium alginate and plant are used again
Object oil carries out double-layer microcapsule processing, obtains microencapsulation probiotics, discharges, avoids beneficial after preparation can be made to reach enteron aisle
Microorganism discharges under one's belt and is destroyed, and improves beneficial microorganism survival rate.
(2) inclusion compound is applied to the processing of microencapsulation probiotics by the present invention for the first time, using special core material and packet
Material formula, by inclusion technique, pellet is granulated and bottom spray packaging technique obtains microencapsulation probiotics, and said preparation not only avoids
Loss of effective component during processing storage transport and feed processing, and can protect effective component, effectively avoid stomach
The destruction and decomposition of sour and various enzymes smoothly reach intestinal colonisation and play beneficial effect, efficiently solve beneficial bacterium processing and answer
The extremely low problem of the utilization rate caused by easily inactivating in the process.
(3) microencapsulation probiotics size distribution of the present invention is good, and mobility is easy to industrial application.
(4) microencapsulation probiotics of the present invention can be directly with feed mixing feeding, and feed processing process losses are less.
(5) present invention first makes microsphere particle with sodium alginate, then carries out the double-deck coating work of vegetable fat coating again
Skill, current microencapsulation probiotics are protected to be created in technique.
Specific embodiment
1, the measuring method of retention rate and dissolution rate:
The microencapsulation probiotics of acquisition are placed under rigor condition (60 DEG C of processing are for 24 hours) to save, before and after detection processing
The lactic acid bacterium number of probiotics, calculating retention rate, retention rate=(number of viable/initial number of viable after processing) ×
100%.
The probiotics of acquisition carry out the measurement of dissolution rate, detection method are as follows:
(1) temperature for adjusting dissolution test system is 37 DEG C, and revolving speed 100r/min pours into artificial preparation in stripping rotor
Simulate the gastric juice, open machine, etc. temperature stablize after, it is spare；
(2) 4g Tiny ecosystem sample (being accurate to 0.0002) is taken, the molten of the dissolution test system debugged in step (1) is put into
Out in cup, sample detection is carried out after 1h, 2h, 4h, 8h, calculates dissolution rate；
The calculation formula of dissolution rate are as follows: dissolution rate=(dissolution content/initial content) × 100%.
2, bacillus subtilis bacterium solution comes from Jiangsu Lv Ke Bioisystech Co., Ltd, lot number 20150811；Acidophilus cream bar
Bacterium solution comes from Jiangsu Lv Ke Bioisystech Co., Ltd, lot number 20150953.
Embodiment 1
A kind of microencapsulation probiotics, including following components and dosage:
Bacillus subtilis bacterium solution 108CFU/ parts, 40 parts of beta-cyclodextrin, 3 parts of PEG400,5 parts of trehalose, microcrystalline cellulose
10 parts, 1 part of sodium alginate, 30 parts of cornstarch, 10 parts of hydrogenated palm oil, 30 parts of dehydrated alcohol.
Above-mentioned microencapsulation probiotics the preparation method comprises the following steps:
(1) it takes bacillus subtilis bacterium solution, PEG400 and the trehalose of above-mentioned dosage to be mixed, carries out ball with beta-cyclodextrin
Mill mixing, revolving speed are 200 turns/min, obtain inclusion compound；
(2) inclusion compound is dried under conditions of 35 DEG C, crushes, cross 60 meshes, then with microcrystalline cellulose, corn
Starch is mixed, and the sodium alginate aqueous solution for being then 0.2% using mass fraction carries out ball blast granulation, control as spray liquid
Revolving speed 15Hz, atomizing pressure 0.15MPa, peristaltic pump flow control obtain the microsphere particle of 30 mesh in 30r/min；
(3) microsphere particle is dried under conditions of 35 DEG C, then with the ethanol solution of hydrogenated palm oil
Bottom spray coating is carried out for coating liquid, 35 DEG C of inlet air temperature, 38 DEG C of outlet air, atomizing pressure 0.15MPa, flow velocity 20r/min are obtained micro-
Encapsulated probiotics.
The retention rate for measuring above-mentioned microencapsulation probiotics is 85.18%, and gastric juice dissolution rate is 9.36%.
Embodiment 2
A kind of microencapsulation probiotics, including following components and dosage:
Lactobacillus acidophilus liquid 107CFU/ parts, 20 parts of beta-cyclodextrin, 5 parts of PEG400,5 parts of trehalose, microcrystalline cellulose 10
Part, 5 parts of sodium alginate, 30 parts of cornstarch, 20 parts of hydrogenated palm oil, 30 parts of dehydrated alcohol.
Above-mentioned microencapsulation probiotics the preparation method comprises the following steps:
(1) it takes lactobacillus acidophilus liquid, PEG400 and the trehalose of above-mentioned dosage to be mixed, carries out ball with beta-cyclodextrin
Mill mixing, 150 turns/min of revolving speed obtain inclusion compound；
(2) inclusion compound is dried under conditions of 35 DEG C, crushes, and crosses 20 meshes, then microcrystalline cellulose, corn form sediment
Powder is mixed, and the sodium alginate aqueous solution for being then 0.2% using mass fraction carries out ball blast granulation as spray liquid, and control turns
Fast 15Hz, atomizing pressure 0.15MPa, peristaltic pump flow control obtain the microsphere particle of 30 mesh in 30r/min；
(3) microsphere particle is dried under conditions of 35 DEG C, then carries out bottom spray and is coated, and 35 DEG C of inlet air temperature,
38 DEG C of outlet air, atomizing pressure 0.15MPa, using the ethanol solution of rilanit special as coating liquid, flow velocity 20r/min is obtained
Microencapsulation probiotics.
The retention rate for measuring above-mentioned microencapsulation probiotics is 86.25%, and gastric juice dissolution rate is 10.45%.
Embodiment 3~7
In addition to the parameter that below table is presented, other content is same as Example 1, prepares microencapsulation Tiny ecosystem system
Agent；Particular content and as a result, as shown in table 1.
Retention rate and gastric juice dissolution rate of the microencapsulation probiotics that the different preparation methods of table 1 obtain under rigor condition
Claims (7)
1. a kind of preparation method of microencapsulation probiotics, which is characterized in that the microencapsulation probiotics, with parts by weight
Number meter, including following components:
The preparation method, comprising:
(1) after mixing lactobacillus suspension, PEG400 and trehalose, ball milling mixing is carried out with beta-cyclodextrin, obtains the β-of lactic acid bacteria
Cyclodextrin inclusion compound；The revolving speed of ball milling is 150~200 revs/min；
(2) inclusion compound is mixed after low-temperature vacuum drying, crushing with microcrystalline cellulose, cornstarch, then through seaweed
Acid sodium aqueous solution spray carries out ball blast granulation, obtains microsphere particle；
(3) microsphere particle carries out bottom spray coating with vegetable oil, obtains microencapsulation Tiny ecosystem system after low-temperature vacuum drying
Agent；The temperature of the low-temperature vacuum drying is 35~40 DEG C；
The condition of bottom spray coating are as follows: inlet air temperature is 30~40 DEG C, and air outlet is 30~40 DEG C, and atomizing pressure is 0.1~0.2
MPa, coating flow velocity are 15~25r/min.
2. the preparation method of microencapsulation probiotics as described in claim 1, which is characterized in that the microencapsulation Tiny ecosystem
Preparation, based on parts by weight, including following components:
3. the preparation method of microencapsulation probiotics as described in claim 1, which is characterized in that the microencapsulation Tiny ecosystem
Preparation, based on parts by weight, including following components:
4. the preparation method of microencapsulation probiotics as described in claim 1, which is characterized in that the vegetable oil is hydrogen
Change palm oil.
5. the preparation method of microencapsulation probiotics as described in claim 1, which is characterized in that the organic solvent is nothing
Water-ethanol.
6. the preparation method of microencapsulation probiotics as described in claim 1, which is characterized in that the item that the ball blast is granulated
Part are as follows: revolving speed is 10~20Hz, and atomizing pressure is 0.1~0.2MPa, and wriggling flow rate pump is 20~30r/min.
7. the preparation method of microencapsulation probiotics as described in claim 1, which is characterized in that the grain of the microsphere particle
Diameter is 30 mesh.
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| CN107373029B (en) * | 2017-08-11 | 2019-10-01 | 中国农业科学院饲料研究所 | A kind of viable bacteria probiotics and its preparation method and application |
| CN111387343A (en) * | 2019-04-09 | 2020-07-10 | 湖北渴望牧业有限责任公司 | High-biological-activity feed additive and preparation method thereof |
| CN115251392A (en) * | 2021-04-29 | 2022-11-01 | 内蒙古伊利实业集团股份有限公司 | Active probiotics, embedding method thereof and application of active probiotics in normal-temperature liquid product |
| CN113142474B (en) * | 2021-05-20 | 2022-08-12 | 四川省食品发酵工业研究设计院有限公司 | Enzyme preparation, method for preparing glutinous rice flour by using enzyme preparation and product thereof |
| CN113678957A (en) * | 2021-08-03 | 2021-11-23 | 大连海洋大学 | Artificial kelp for feeding sea urchin ball and preparation method thereof |
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| CN102178238A (en) * | 2011-03-02 | 2011-09-14 | 润盈生物工程（上海）有限公司 | Heat-resistance microencapsulated probiotics and preparation method and use thereof |
| CN104004670A (en) * | 2014-05-29 | 2014-08-27 | 广东省农业科学院动物科学研究所 | Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof |
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| KR100777210B1 (en) * | 2006-05-29 | 2007-11-28 | (주)케비젠 | The method of preparing lactic acid bacteria-unsaturated fatty acid microcapsule-krill powder, the powder prepared by the method, and the spices comprising the powder |
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| CN1569043A (en) * | 2003-07-17 | 2005-01-26 | 中国农业大学 | Coated micro capsule of lactic acid bacteria and its preparation |
| CN102178238A (en) * | 2011-03-02 | 2011-09-14 | 润盈生物工程（上海）有限公司 | Heat-resistance microencapsulated probiotics and preparation method and use thereof |
| CN104004670A (en) * | 2014-05-29 | 2014-08-27 | 广东省农业科学院动物科学研究所 | Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof |
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