CN113528614B - 一种植物叶际表面微生物宏基因组检测方法 - Google Patents
一种植物叶际表面微生物宏基因组检测方法 Download PDFInfo
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- CN113528614B CN113528614B CN202110808262.0A CN202110808262A CN113528614B CN 113528614 B CN113528614 B CN 113528614B CN 202110808262 A CN202110808262 A CN 202110808262A CN 113528614 B CN113528614 B CN 113528614B
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Abstract
Description
温度 | 时间 | |
预变性 | 94℃ | 3min |
变性 | 94℃ | 40s |
退火 | 42℃ | 45s |
延伸 | 72℃ | 50s(36个循环) |
延伸 | 72℃ | 6min |
温度 | 时间 | |
预变性 | 94℃ | 3min |
变性 | 94℃ | 40s |
退火 | 42℃ | 45s |
延伸 | 72℃ | 50s(36个循环) |
延伸 | 72℃ | 6min |
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CN113921089B (zh) * | 2021-11-22 | 2022-04-08 | 北京安智因生物技术有限公司 | 一种用于确认ivd基因注释数据库更新频率的方法及系统 |
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CN104389026A (zh) * | 2014-10-30 | 2015-03-04 | 北京诺禾致源生物信息科技有限公司 | 单细胞转录组测序文库的构建方法及其应用 |
CN106480213A (zh) * | 2016-11-30 | 2017-03-08 | 江西中烟工业有限责任公司 | 陈化烟叶表面微生物的宏基因组测序与鉴定方法 |
CN111269909A (zh) * | 2018-12-04 | 2020-06-12 | 深圳华大基因科技服务有限公司 | 一种转录组建库的方法、试剂和应用 |
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