CN113490683A - 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 - Google Patents
灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 Download PDFInfo
- Publication number
- CN113490683A CN113490683A CN202080013982.1A CN202080013982A CN113490683A CN 113490683 A CN113490683 A CN 113490683A CN 202080013982 A CN202080013982 A CN 202080013982A CN 113490683 A CN113490683 A CN 113490683A
- Authority
- CN
- China
- Prior art keywords
- plant
- plants
- disease resistance
- transgenic
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 78
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 45
- 241000196324 Embryophyta Species 0.000 claims abstract description 380
- 230000009261 transgenic effect Effects 0.000 claims abstract description 167
- 240000008042 Zea mays Species 0.000 claims abstract description 78
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 78
- 208000035240 Disease Resistance Diseases 0.000 claims abstract description 73
- 201000010099 disease Diseases 0.000 claims abstract description 56
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 56
- 238000000034 method Methods 0.000 claims abstract description 44
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims abstract description 41
- 235000009973 maize Nutrition 0.000 claims abstract description 41
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 37
- 235000005822 corn Nutrition 0.000 claims abstract description 37
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 31
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 31
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 31
- 238000009395 breeding Methods 0.000 claims abstract description 8
- 230000001488 breeding effect Effects 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims abstract description 6
- 230000001965 increasing effect Effects 0.000 claims abstract description 5
- 238000003976 plant breeding Methods 0.000 claims abstract description 5
- 108020004414 DNA Proteins 0.000 claims description 29
- 102000053602 DNA Human genes 0.000 claims description 22
- 241001157813 Cercospora Species 0.000 claims description 19
- 239000013598 vector Substances 0.000 claims description 13
- 239000002773 nucleotide Substances 0.000 claims description 12
- 125000003729 nucleotide group Chemical group 0.000 claims description 12
- 241000209149 Zea Species 0.000 claims description 8
- 108091026890 Coding region Proteins 0.000 claims description 6
- 230000014509 gene expression Effects 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 108020001507 fusion proteins Proteins 0.000 claims description 3
- 102000037865 fusion proteins Human genes 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 125000000539 amino acid group Chemical group 0.000 claims description 2
- 235000013339 cereals Nutrition 0.000 description 30
- 239000000463 material Substances 0.000 description 30
- 238000012360 testing method Methods 0.000 description 18
- 238000012216 screening Methods 0.000 description 16
- 238000003306 harvesting Methods 0.000 description 15
- 239000013604 expression vector Substances 0.000 description 13
- 230000002068 genetic effect Effects 0.000 description 13
- 241000589158 Agrobacterium Species 0.000 description 12
- 230000003321 amplification Effects 0.000 description 12
- 238000003199 nucleic acid amplification method Methods 0.000 description 12
- 239000013612 plasmid Substances 0.000 description 12
- 238000009396 hybridization Methods 0.000 description 10
- 238000003259 recombinant expression Methods 0.000 description 10
- 230000009466 transformation Effects 0.000 description 8
- 238000012258 culturing Methods 0.000 description 7
- 210000001161 mammalian embryo Anatomy 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 4
- 108700026244 Open Reading Frames Proteins 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108020005350 Initiator Codon Proteins 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000010200 validation analysis Methods 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 101100119888 Arabidopsis thaliana FDM2 gene Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108091033409 CRISPR Proteins 0.000 description 1
- 101150067473 IDP2 gene Proteins 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 241000221576 Uromyces Species 0.000 description 1
- 241000981775 Urospora <green alga> Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 238000010362 genome editing Methods 0.000 description 1
- 101150046722 idh1 gene Proteins 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
Abstract
本发明公开了一种灰斑病抗性相关蛋白ZmWAK‑RLK及其编码基因和应用。本发明提供的蛋白质,获自玉米自交系Y32,命名为ZmWAK‑RLK蛋白,为序列表中序列1所示的蛋白质。编码ZmWAK‑RLK蛋白的核酸分子也属于本发明的保护范围。本发明还保护一种制备转基因植物的方法,包括如下步骤:在出发植物中导入所述核酸分子,得到灰斑病抗病性增强的转基因植物。本发明还保护一种植物育种方法,包括如下步骤:增加目的植物中ZmWAK‑RLK蛋白的含量和/或活性,从而提高目的植物的灰斑病抗病性。本发明对于玉米的抗灰斑病育种具有重大的应用价值。
Description
PCT国内申请,说明书已公开。
Claims (1)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2019101404791 | 2019-02-26 | ||
CN201910140479.1A CN109705200A (zh) | 2019-02-26 | 2019-02-26 | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 |
PCT/CN2020/076320 WO2020173403A1 (zh) | 2019-02-26 | 2020-02-24 | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113490683A true CN113490683A (zh) | 2021-10-08 |
CN113490683B CN113490683B (zh) | 2024-03-26 |
Family
ID=66263917
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910140479.1A Pending CN109705200A (zh) | 2019-02-26 | 2019-02-26 | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 |
CN202080013982.1A Active CN113490683B (zh) | 2019-02-26 | 2020-02-24 | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910140479.1A Pending CN109705200A (zh) | 2019-02-26 | 2019-02-26 | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 |
Country Status (6)
Country | Link |
---|---|
US (1) | US12077769B2 (zh) |
EP (1) | EP3932939A4 (zh) |
CN (2) | CN109705200A (zh) |
BR (1) | BR112021016750A2 (zh) |
MX (1) | MX2021010318A (zh) |
WO (1) | WO2020173403A1 (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109705200A (zh) * | 2019-02-26 | 2019-05-03 | 中国农业大学 | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 |
CN114262369B (zh) * | 2021-12-15 | 2023-05-16 | 中国农业大学 | ZmDi19基因及其靶标基因ZmPR10在培育抗灰斑病植物中的应用 |
CN114907461B (zh) * | 2022-03-30 | 2023-08-01 | 中国农业科学院作物科学研究所 | 灰斑病抗性相关蛋白ZmPMT1及其编码基因和应用 |
CN114854712B (zh) * | 2022-06-08 | 2023-08-11 | 华中农业大学 | 玉米ZmWAK02基因在提高玉米灰斑病抗性中的应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104341494A (zh) * | 2013-08-09 | 2015-02-11 | 中国农业大学 | 一种高抗丝黑穗病的蛋白ZmWAK及其编码基因和其应用 |
CN106701972A (zh) * | 2017-01-23 | 2017-05-24 | 华中农业大学 | 玉米抗灰斑病主效qtl的连锁分子标记和应用 |
CN109705200A (zh) * | 2019-02-26 | 2019-05-03 | 中国农业大学 | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103987848A (zh) * | 2011-10-21 | 2014-08-13 | 巴斯夫植物科学有限公司 | 具有增强的产量相关性状的植物及其制备方法 |
US20150315605A1 (en) * | 2014-02-21 | 2015-11-05 | E I Du Pont De Nemours And Company | Novel transcripts and uses thereof for improvement of agronomic characteristics in crop plants |
US20170114356A1 (en) * | 2015-02-20 | 2017-04-27 | E I Du Pont De Nemours And Company | Novel alternatively spliced transcripts and uses thereof for improvement of agronomic characteristics in crop plants |
CN105859860A (zh) * | 2016-05-18 | 2016-08-17 | 中国科学院遗传与发育生物学研究所 | 抗病相关蛋白在调控植物抗病性中的应用 |
BR112019000579A2 (pt) | 2016-07-11 | 2019-07-02 | Pioneer Hi Bred Int | métodos de identificação e/ou seleção de uma planta de milho que tem ou exibe resistência aumentada à mancha de folha cinza |
CN109609676B (zh) | 2018-12-25 | 2021-12-14 | 袁隆平农业高科技股份有限公司 | 与玉米抗灰斑病主效QTL-qRgls1共分离的SNP标记及应用 |
-
2019
- 2019-02-26 CN CN201910140479.1A patent/CN109705200A/zh active Pending
-
2020
- 2020-02-24 CN CN202080013982.1A patent/CN113490683B/zh active Active
- 2020-02-24 WO PCT/CN2020/076320 patent/WO2020173403A1/zh unknown
- 2020-02-24 EP EP20762082.4A patent/EP3932939A4/en active Pending
- 2020-02-24 BR BR112021016750A patent/BR112021016750A2/pt unknown
- 2020-02-24 US US17/433,159 patent/US12077769B2/en active Active
- 2020-02-24 MX MX2021010318A patent/MX2021010318A/es unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104341494A (zh) * | 2013-08-09 | 2015-02-11 | 中国农业大学 | 一种高抗丝黑穗病的蛋白ZmWAK及其编码基因和其应用 |
CN106701972A (zh) * | 2017-01-23 | 2017-05-24 | 华中农业大学 | 玉米抗灰斑病主效qtl的连锁分子标记和应用 |
CN109705200A (zh) * | 2019-02-26 | 2019-05-03 | 中国农业大学 | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 |
Non-Patent Citations (5)
Title |
---|
GENBANK: "PWZ09400.1,Rust resistance kinase Lr10 [Zea mays]", 《GENBANK》 * |
GENBANK: "PWZ09400.1,Rust resistance kinase Lr10 [Zea mays]", 《GENBANK》, 4 June 2018 (2018-06-04) * |
崔凤超: "基于混池测序的抗玉米灰斑病QTL定位", 《生物技术进展》 * |
崔凤超: "基于混池测序的抗玉米灰斑病QTL定位", 《生物技术进展》, 25 July 2018 (2018-07-25) * |
钟涛: "玉米灰斑病和茎腐病抗病基因克隆及抗病机理研究", 中国博士学位论文全文数据库农业科技辑, pages 2 * |
Also Published As
Publication number | Publication date |
---|---|
CN113490683B (zh) | 2024-03-26 |
CN109705200A (zh) | 2019-05-03 |
EP3932939A1 (en) | 2022-01-05 |
US20220177907A1 (en) | 2022-06-09 |
EP3932939A4 (en) | 2022-12-14 |
BR112021016750A2 (pt) | 2021-11-03 |
MX2021010318A (es) | 2021-09-23 |
US12077769B2 (en) | 2024-09-03 |
WO2020173403A1 (zh) | 2020-09-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Wu et al. | Development of enhancer trap lines for functional analysis of the rice genome | |
EP3186381B1 (en) | Generation of haploid plants | |
US9663794B2 (en) | Heat-resistance rice gene OsZFP, screening marker and separation method thereof | |
US8940961B2 (en) | Plant transformed with hardy (HRD) gene having enhanced drought tolerance | |
CN113490683A (zh) | 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用 | |
CN109705202B (zh) | 一种培育抗灰斑病植物的方法 | |
CN104769114A (zh) | Rhg1介导的对大豆胞囊线虫的抗性 | |
CN109112146B (zh) | 控制甘蓝型油菜角果长和粒重性状的基因qSLWA9的克隆与育种应用 | |
US20220106607A1 (en) | Gene for parthenogenesis | |
US20090031444A1 (en) | Homologous recombination in plants | |
Biswas et al. | Genetic characterization of mutants resistant to the antiauxin p-chlorophenoxyisobutyric acid reveals that AAR3, a gene encoding a DCN1-like protein, regulates responses to the synthetic auxin 2, 4-dichlorophenoxyacetic acid in Arabidopsis roots | |
CN109295246B (zh) | 与玉米雄性生育力相关的dna分子标记及其应用 | |
CN108864266B (zh) | 一种与水稻落粒性及粒型相关的蛋白ssh1及其编码基因与应用 | |
CN112725374A (zh) | 创制植物单倍体诱导系的方法及其应用 | |
Li et al. | Generation of marker-free transgenic rice resistant to rice blast disease using Ac/Ds transposon-mediated transgene reintegration system | |
CN118185987A (zh) | 水稻基因OsCWIN5及其应用 | |
AU2004210748A1 (en) | Gene resistant to Aphis gossypii | |
CN110407921B (zh) | 来源于谷子的植物籽粒发育相关蛋白sgdw1及其编码基因和应用 | |
CN114946639A (zh) | 两种小麦诱导系在小麦单倍体幼胚及籽粒鉴别中的应用 | |
CN111763249B (zh) | 植物白粉病抗性相关蛋白Pm5e及其编码基因和应用 | |
Gao et al. | ZmXa21-L gene encodes a plant receptor-like kinases (RLKs) protein that enhances resistance to bacterial blight in rice | |
CN111909250A (zh) | 蛋白质invan6、其编码基因以及它们在选育玉米雄性不育系中的应用 | |
WO2024174954A1 (zh) | 小麦广谱抗白粉病基因wtk7-tm克隆、功能标记及其应用 | |
CN108866095B (zh) | 植物抗逆相关蛋白ZmHSP3及其编码基因与应用 | |
CN114672493B (zh) | 一种用ZmPHT1;7蛋白或其编码基因培育抗旱植物的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |