CN113398001B

CN113398001B - Composition containing hyaluronic acid and preparation method and application thereof

Info

Publication number: CN113398001B
Application number: CN202110706879.1A
Authority: CN
Inventors: 钱捷豪
Original assignee: Zhuhai Yasha Medical Devices Co ltd
Current assignee: Zhuhai Yasha Medical Devices Co ltd
Priority date: 2021-06-24
Filing date: 2021-06-24
Publication date: 2022-10-18
Anticipated expiration: 2041-06-24
Also published as: CN113398001A

Abstract

The invention belongs to the technical field of cosmetics, and discloses a composition containing hyaluronic acid, and a preparation method and application thereof. The composition containing hyaluronic acid comprises hyaluronic acid, eucalyptus oil, squalene and geranium oil. The composition containing hyaluronic acid provided by the invention takes hyaluronic acid as a basic raw material, is matched with squalene, eucalyptus oil and geranium oil, and has synergistic effect, so that the proliferation and differentiation of keratinocytes can be promoted, the skin micro-ecology can be improved, and the skin is in a weakly acidic healthy state; the repaired skin does not have dependence on the composition, and can keep good water retention for a long time.

Description

Composition containing hyaluronic acid and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a composition containing hyaluronic acid, and a preparation method and application thereof.

Background

The skin barrier is an important defense line for protecting the human body, and can be composed of a water lipid membrane, epidermal lipids, antibacterial peptides, microecological cells, keratinocytes and the like. Keratinocytes themselves, by regulating water flux and retention, combat environmental disadvantages such as dryness, and are among the most important protective structural components of the stratum corneum. The whole horny layer can resist the invasion of external microorganisms, and the water lipid membrane and the antibacterial peptide are physiological structures and components with antibacterial and bactericidal capabilities for healthy skin. However, the skin barrier is damaged more and more due to environmental pollution, improper use of cosmetics, etc., which results in skin with uncomfortable symptoms such as incomplete barrier, low tolerance, redness and itching, and dry skin.

At present, a lot of cosmetics are used for skin moisturizing or skin barrier repair, and the main raw materials comprise the following types: 1. the hygroscopic raw materials comprise glycerin, butanediol, polysaccharides, sodium lactate and the like, absorb water from the environment, and form a water concentration gradient in the skin cutin layer so as to supplement the water lost by the cutin layer; 2. the sealing raw materials comprise fatty acid, vaseline, shea butter, etc., and can form a hydrophobic thin oil film on the skin surface, so as to reinforce the skin barrier; 3. the bionic raw materials which are the same as or similar to the components of the epidermis and the dermis, such as natural moisturizing factors, ceramide and collagen, supplement the natural components of the skin, enhance the self moisturizing capability of the skin and have the function of repairing the skin barrier. Although these cosmetics and dressings for repairing skin barrier can repair skin to some extent when used, many of them have problems such as allergy and dryness immediately after they are stopped, and they cause serious skin dependency. This is true because the effect of repairing the skin barrier is not fundamentally achieved.

Thus, there is a need to provide a composition for repairing the skin barrier that is effective, has sustained effect, and does not cause skin dependency.

Disclosure of Invention

The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides a composition containing hyaluronic acid, which can effectively repair skin barriers and does not cause skin dependence.

The invention conception is as follows: the inventor finds that the skin barrier can be effectively repaired by researching the skin barrier tissue and maintaining good skin micro-ecology and promoting the proliferation and differentiation of keratinocytes. In experiments, the inventor finds that the hyaluronic acid is matched with squalene, eucalyptus oil and geranium oil, so that the proliferation and differentiation of keratinocytes can be promoted, the skin micro-ecology can be improved, and the skin is in a weakly acidic healthy state; and the repaired skin does not have dependence on the composition and can keep good water retention (the capability of the skin to keep self water) for a long time.

In a first aspect, the present invention provides a composition comprising hyaluronic acid.

Specifically, the composition containing hyaluronic acid comprises hyaluronic acid, eucalyptus oil, squalene and geranium oil.

The composition is prepared by compounding hyaluronic acid with squalene, eucalyptus oil and geranium oil, wherein the hyaluronic acid is taken as a basic raw material, permeates between epidermis and dermis, is combined with protein to promote cell adhesion, and has the functions of moisturizing. The eucalyptus oil can promote the proliferation and differentiation of keratinocytes, increase the ceramide content in the horny layer (ceramide is a main substance for preventing water loss), and improve the water retention of the skin by cooperating with hyaluronic acid; the eucalyptus oil can remove oxygen free radicals, reduce lipid peroxidation, and reduce or prevent apoptosis; eucalyptus oil also has antibacterial and antifungal effects, and can lower the skin microbial population at a stable level. Squalene, as one of lipid secreted by sebaceous gland, can increase skin lipid content, improve skin surface microenvironment, and maintain skin microbiota (such as Staphylococcus epidermidis) stable state under acidic condition; meanwhile, due to the special chemical structure of squalene, squalene has the effects of moisturizing and resisting oxidation. The geranium oil can reduce neurovascular reactivity, improve skin sensitivity and reduce skin dependence on hyaluronic acid, squalene and eucalyptus oil. The composition can effectively repair skin barriers under the combined action of the four components, does not cause skin dependence, and can keep the repaired skin with good water retention for a long time.

Preferably, the ratio of the mass of the hyaluronic acid to the total mass of the eucalyptus oil, squalene and geranium oil is (5-20): 1; further preferably, the ratio of the mass of the hyaluronic acid to the total mass of the eucalyptus oil, squalene and geranium oil is (8-20): 1; more preferably, the ratio of the mass of the hyaluronic acid to the total mass of the eucalyptus oil, squalene and geranium oil is (10-15): 1.

Preferably, the mass ratio of the eucalyptus oil to the squalene to the geranium oil is 1: (0.5-2): (0.5-2); further preferably, the mass ratio of the eucalyptus oil, the squalene and the geranium oil is 1: (0.5-1.5): (0.5-1.5); more preferably, the mass ratio of the eucalyptus oil, the squalene and the geranium oil is 1: (0.5-1): (0.5-1).

Preferably, the composition further comprises tremella polysaccharide. The tremella polysaccharide can absorb water which is dozens of times to hundreds of times of the weight of the tremella polysaccharide, after the tremella polysaccharide absorbs water, sugar chains are opened to form a space network structure, intermolecular hydrogen bonding effect and the network sugar chain structure have excellent water binding capacity, and water replenishing and moisture retention of hyaluronic acid are further improved; meanwhile, the tremella polysaccharide mainly takes xylose, fucose, glucuronic acid and glucose as branched chains and mannose as a main chain, and a three-dimensional network structure is formed, so that the tremella polysaccharide has good film forming property, and is beneficial to absorption of squalene, eucalyptus oil and geranium oil, and loss of the squalene, the eucalyptus oil and the geranium oil is prevented.

Preferably, the mass ratio of the tremella polysaccharides to the hyaluronic acid is 1: (8-30); further preferably, the mass ratio of the tremella polysaccharide to the hyaluronic acid is 1: (10-20). By controlling the proportion of the water replenishing and the water locking, the effect of water replenishing and locking can be optimal.

Preferably, the composition further comprises a plant essential oil selected from at least one of oat oil, sunflower seed oil, coconut oil or jojoba oil.

Further preferably, the plant essential oils are sunflower oil and oat oil, the sunflower oil can promote keratinocyte proliferation and lipid synthesis, and the oat oil can induce keratinocyte differentiation and ceramide synthesis to jointly improve skin barrier function.

Preferably, the composition comprises, in parts by weight: 60-180 parts of hyaluronic acid, 1-5 parts of eucalyptus oil, 1-5 parts of squalene, 1-5 parts of geranium oil, 5-10 parts of tremella polysaccharide, 1-5 parts of sunflower seed oil and 1-5 parts of oat oil.

In a second aspect, the invention provides a method for preparing a composition comprising hyaluronic acid.

Specifically, the preparation method of the composition containing hyaluronic acid comprises the following steps: the components are weighed and mixed to produce the composition.

In a third aspect, the invention provides the use of a composition comprising hyaluronic acid.

The use of the hyaluronic acid-containing composition for the preparation of a product for repairing a skin barrier.

A dressing comprising the hyaluronic acid-containing composition.

Preferably, the dressing further comprises water and an adjuvant.

Preferably, the auxiliary agent is selected from at least one of an emulsifier, a thickener, a preservative or a pH adjuster.

Preferably, the emulsifier is at least one selected from glycerol stearate (a 165), cetearyl glucoside, arachidyl glucoside and behenyl glucoside.

Preferably, the thickener is selected from at least one of carbomer, cellulose or xanthan gum.

Preferably, the preservative is selected from 1, 2-pentanediol and/or 1, 2-hexanediol. The 1, 2-pentanediol and the 1, 2-hexanediol have the bacteriostatic action, can prevent corrosion, have the moisturizing action and can enhance the moisturizing performance of the dressing.

Preferably, the pH adjusting agent is citric acid and/or arginine.

A cosmetic contains the hyaluronic acid-containing composition.

Preferably, the cosmetic comprises the hyaluronic acid-containing composition, water and an adjuvant.

The cosmetics comprise astringent, lotion, cream, facial mask and the like.

Preferably, the auxiliary agent is selected from at least one of an emulsifier, a defoamer, a thickener, a preservative or a pH adjuster. The content of the preservative in the auxiliary agent accounts for less than 1% of the total mass of the dressing. The addition amount of each component in the auxiliary agent meets the requirement of the cosmetic industry standard.

Preferably, the defoamer is selected from dimethicone or silicone oil.

Preferably, the preservative is selected from 1, 2-pentanediol and/or 1, 2-hexanediol.

Preferably, the pH adjusting agent is citric acid and/or arginine.

Compared with the prior art, the invention has the following beneficial effects:

the composition containing hyaluronic acid provided by the invention takes hyaluronic acid as a basic raw material, is matched with squalene, eucalyptus oil and geranium oil, and has synergistic effect, so that the proliferation and differentiation of keratinocytes can be promoted, the skin micro-ecology can be improved, and the skin is in a weakly acidic healthy state; the repaired skin does not have dependence on the composition, and can keep good water retention for a long time.

Detailed Description

In order to make the technical solutions of the present invention more apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are not intended to limit the scope of the claimed invention.

The starting materials, reagents or apparatuses used in the following examples are conventionally commercially available or can be obtained by conventionally known methods, unless otherwise specified.

Example 1

A hyaluronic acid-containing composition comprising:

a method for preparing a composition comprising hyaluronic acid, comprising the steps of:

accurately weighing the components, mixing hyaluronic acid and Tremella polysaccharide, adding eucalyptus oil, squalene, geranium oil, sunflower seed oil and oat oil, stirring at 50rpm below 25 deg.C for 8 hr, filtering, and removing residue to obtain the composition.

Example 2

A hyaluronic acid-containing composition comprising:

Example 3

A hyaluronic acid-containing composition comprising:

accurately weighing each component, mixing hyaluronic acid and Tremella polysaccharide, adding eucalyptus oil, squalene, geranium oil, sunflower seed oil and oat oil, stirring and mixing at 50rpm below 25 deg.C for 8 hr, filtering, and removing residue to obtain the composition.

Example 4

A hyaluronic acid-containing composition comprising:

Example 5

A hyaluronic acid-containing composition comprising:

accurately weighing the components, mixing hyaluronic acid and Tremella polysaccharide, adding eucalyptus oil, squalene and geranium oil, stirring at 50rpm below 25 deg.C for 8 hr, filtering, and removing residue to obtain the composition.

Example 6

A hyaluronic acid-containing composition comprising:

accurately weighing the components, mixing hyaluronic acid and Tremella polysaccharide, adding eucalyptus oil, squalene, geranium oil, coconut oil and jojoba oil, stirring at 50rpm below 25 deg.C for 8 hr, filtering, and removing residue to obtain the composition.

Example 7

A dressing for repairing skin barrier comprises 7 parts of hyaluronic acid-containing composition prepared in example 1, 1 part of arginine, 2 parts of arachidyl alcohol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum and 87.5 parts of water.

Example 8

A dressing for repairing skin barrier comprises 7 parts of hyaluronic acid-containing composition prepared in example 2, 1 part of arginine, 2 parts of arachidyl alcohol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum and 87.5 parts of water.

Example 9

A dressing for repairing skin barrier comprises 7 parts of hyaluronic acid-containing composition prepared in example 3, 1 part of arginine, 2 parts of arachidyl alcohol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum and 87.5 parts of water.

Example 10

A dressing for repairing skin barrier comprises 7 parts of hyaluronic acid-containing composition prepared in example 4, 1 part of arginine, 2 parts of arachidyl alcohol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum and 87.5 parts of water.

Example 11

A dressing for repairing skin barrier comprises 7 parts of hyaluronic acid-containing composition prepared in example 5, 1 part of arginine, 2 parts of arachidyl alcohol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum and 87.5 parts of water.

Example 12

A dressing for repairing skin barrier comprises 7 parts of hyaluronic acid-containing composition prepared in example 6, 1 part of arginine, 2 parts of arachidyl alcohol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum and 87.5 parts of water.

Comparative example 1

Comparative example 1 is different from example 1 in that 120 parts of glycerin is used instead of hyaluronic acid, and the remaining components and preparation method are the same as example 1.

Comparative example 2

Comparative example 2 differs from example 1 in that instead of eucalyptus oil, 2 parts of squalene and 2 parts of geranium oil are used, the remaining components and the preparation being identical to example 1.

Comparative example 3

Comparative example 3 differs from example 1 in that 1.5 parts of eucalyptus oil and 1.5 parts of geranium oil are used instead of squalene, the remaining components and the preparation method being the same as in example 1.

Comparative example 4

Comparative example 4 differs from example 1 in that 1.5 parts of eucalyptus oil and 1.5 parts of squalene are used instead of geranium oil, the remaining components and the preparation method being the same as in example 1.

Comparative example 5

A dressing comprising 7 parts of the hyaluronic acid-free composition prepared in comparative example 1, 1 part of arginine, 2 parts of arachidonol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum, and 87.5 parts of water.

Comparative example 6

A dressing comprising 7 parts of the hyaluronic acid-containing composition prepared in comparative example 2, 1 part of arginine, 2 parts of arachidonol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum, and 87.5 parts of water.

Comparative example 7

A dressing comprising 7 parts of the hyaluronic acid-containing composition prepared in comparative example 3, 1 part of arginine, 2 parts of arachidonol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum, and 87.5 parts of water.

Comparative example 8

A dressing comprising 7 parts of the hyaluronic acid-containing composition prepared in comparative example 4, 1 part of arginine, 2 parts of arachidonol glucoside, 0.5 part of 1, 2-pentanediol, 2 parts of xanthan gum, and 87.5 parts of water.

Product effectiveness testing

1. Haemolysis test of erythrocytes

In the erythrocyte hemolysis test, the damage of chemicals to the eye tissue cells is evaluated by measuring the amount of dissolved hemoglobin and the degree of denaturation. The eye irritation of cosmetics and raw materials thereof is evaluated internationally mainly by erythrocyte hemolysis experiments.

The compositions prepared in examples 1 to 6 and comparative examples 1 to 4 were subjected to an erythrocyte hemolysis test and the irritation of the compositions was evaluated. The irritation grading criteria are shown in Table 1.

TABLE 1 irritation grading Standard

	L/D	Grading
			1	L/D＞100	Has no irritation
2	10＜L/D≤100	Micro-stimulation property
			3	1＜L/D≤10	Mild irritation
4	0.1＜L/D≤1	Moderate irritation
			5	L/D≤0.1	Severe irritation

The results of the erythrocyte hemolysis test of the compositions prepared in examples 1 to 6 of the present invention and comparative examples 1 to 4 are shown in Table 2.

TABLE 2 results of erythrocyte hemolysis experiment

	HC50(mg/L)	DI(％)	L/D	Evaluation of
					Example 1	21319	99.38	＞100	Has no irritation
Example 2	21915	99.45	＞100	Has no irritation
					Example 3	23503	99.97	＞100	Has no irritation
Example 4	20548	98.65	＞100	Has no irritation
					Example 5	23547	99.99	＞100	Has no irritation
Example 6	21412	99.12	＞100	Has no irritation
					Comparative example 1	21658	99.56	＞100	Has no irritation
Comparative example 2	21549	99.42	＞100	Has no irritation
					Comparative example 3	19673	98.12	＞100	Has no irritation
Comparative example 4	20045	98.33	＞100	Has no irritation

In table 2, HC50 (mg/L) is the concentration of the test substance that causes hemolysis of 50% in erythrocytes, DI (%) hemoglobin denaturation index, L/D = HC50/DI. As can be seen from Table 2, the compositions prepared in the examples of the present invention are non-irritating, safe and reliable.

2. Skin repair efficacy test

The dressings provided in examples 7 to 12 and comparative examples 5 to 8 were subjected to a moisturizing performance test to test the rate of change of transdermal water loss: 100 volunteers of 20-40 years old with damaged skin (manifested by dry skin, peeling or red blood streaks due to environmental influences or improper use of cosmetics), male and female ratio 4, 6 were selected and randomly divided into 10 groups of 4 men and 6 women, using the dressings provided in examples 7-12 and comparative examples 5-8, respectively. The specific application method of the dressing comprises the following steps: the subjects were rested for 10 minutes before testing, then 10g of dressing was applied evenly to the subjects' faces, and after 20 minutes the unabsorbed dressing was rinsed off with clear water and wiped dry. Each group was tested for the rate of change in transepidermal water loss (100% based on the amount of water lost before the dressing was not used) in each subject using a German CK skin Water loss tester TM300 (TEWL) before the dressing was not used, and after 15 and 30 consecutive days. And (3) testing environment: room temperature 25 ℃ and humidity 60%. Each data is tested three times, after the average value is obtained, the average value of each group is calculated, and one digit behind the decimal point is reserved. The test results are shown in Table 3.

TABLE 3 Perdermally water loss Rate of Change for each group

The transdermal water loss rate (TEWL) is one of the main methods for judging the skin barrier function, and it can be seen from table 3 that the transdermal water loss rate (TEWL) of the subject was significantly decreased compared to that before the use, the skin barrier was effectively repaired, and the effect was significantly better than that of the comparative example, after the dressings prepared in examples 7 to 12 were used.

3. Skin repair and stability testing

Testing the water content, pH value and oil content of the skin: 100 volunteers of 20-40 years old with damaged skin (manifested by dry skin, peeling or red blood streaks due to environmental influences or improper use of cosmetics), male and female ratio 4, 6 were selected and randomly divided into 10 groups of 4 men and 6 women, using the dressings provided in examples 7-12 and comparative examples 5-8, respectively. The dressing was stopped after 30 consecutive days for each group of dressings, and the skin was observed and tested 1, 7, and 15 days after the stoppage.

The specific using method comprises the following steps: before the test, the testees sit still for 10 minutes, then 10g of dressing is taken and evenly coated on the faces of the testees, after 20 minutes, the unabsorbed dressing is washed away by clean water and wiped dry, and after sitting still for one hour, a skin oil, moisture and pH tester is adopted to test the water content, the oil content and the pH value of the skin of each testee.

Calculating the change rate of the water content of the skin and the change rate of the oil content of the skin: and calculating the change rate of the water content of the skin and the change rate of the oil content of the skin by taking the water content and the oil content of the skin before dressing is not used as initial values of the water content and the oil content of the skin. Wherein, the skin moisture content change rate = skin moisture content/skin moisture content initial value 100% after n days of use; skin oil content change rate = skin oil content/initial value of skin oil content 100% after n days of use (use n days includes use for 30 days, 1, 7, 15 days after use has ceased). Each data is tested three times, after the average value is obtained, the average value of each group is calculated, and one digit behind the decimal point is reserved. The results of the skin moisture content change rate are shown in table 4, the results of the skin pH value test are shown in table 5, and the results of the skin oil content change rate test are shown in table 6.

TABLE 4 rate of change of skin moisture content

As can be seen from Table 4, the moisture content of the skin increases by 60-80% after the dressing provided by the embodiment of the invention is continuously used for 30 days, the skin is well repaired, and the moisture retention is good. When the use is stopped, the moisture content of the skin can be basically kept unchanged, and the dependence on the dressing after the skin is repaired is small. After the dressing provided by the comparative example of the invention is used, the change rate of the moisture content of the skin is smaller than that of the dressing in the example, and after the dressing is stopped for 15 days, the moisture content of the skin is obviously reduced, the skin repairing effect is poor, and the dressing has certain dependence.

TABLE 5 skin pH

As can be seen from table 5, the pH of the skin of the dressing provided by the embodiment of the present invention decreases to 6.1-6.3 after 30 days of continuous use, and the pH of the skin is maintained at a more stable level after the dressing is stopped, while the pH of the dressing in the comparative example fluctuates greatly.

TABLE 6 rate of change of skin oil content

As can be seen from Table 6, the change rate of the oil content of the skin is increased by 35-50% after the dressing provided by the embodiment of the invention is continuously used for 30 days, and the skin is well repaired. And after the use is stopped, the oil content of the skin can be basically kept unchanged, and the dependence on the dressing after the skin is repaired is small. After the dressing provided by the comparative example of the invention is used, the change rate of the skin oil content is smaller than that of the dressing in the example, and after the dressing is stopped for 15 days, the skin oil content is obviously reduced, the skin repairing effect is poor, and the dressing has certain dependence.

Similar effects can be obtained by preparing a cosmetic water, lotion, cream, pack using the hyaluronic acid-containing compositions prepared in examples 1 to 6.

Claims

1. A composition containing hyaluronic acid for repairing skin barrier is characterized by being prepared from the following raw material components: hyaluronic acid, eucalyptus oil, squalene, geranium oil and tremella polysaccharide;

the ratio of the mass of the hyaluronic acid to the total mass of the eucalyptus oil, the squalene and the geranium oil is (10-15): 1;

the mass ratio of the eucalyptus oil to the squalene to the geranium oil is 1: (0.5-2): (0.5-2);

the mass ratio of the tremella polysaccharide to the hyaluronic acid is 1: (10-20).

2. A composition containing hyaluronic acid for repairing skin barrier is characterized by being prepared from the following raw material components: hyaluronic acid, eucalyptus oil, squalene, geranium oil, tremella polysaccharide and plant essential oil; the plant essential oil is at least one of oat oil, sunflower seed oil, coconut oil or jojoba oil;

the mass ratio of the eucalyptus oil, the squalene and the geranium oil is 1: (0.5-2): (0.5-2);

3. A process for the preparation of a composition according to any one of claims 1 to 2, characterized in that it comprises the following steps:

the components are weighed and mixed to produce the composition.

4. Use of a composition according to any one of claims 1-2 for the preparation of a product for repairing skin barriers.

5. A dressing for repairing a skin barrier comprising the composition of any one of claims 1-2.

6. A cosmetic product for repairing the skin barrier, characterized in that it comprises a composition according to any one of claims 1 to 2.