CN113229055A - Wild-imitating cultivation method for pilose antler grass in mountainous region - Google Patents

Wild-imitating cultivation method for pilose antler grass in mountainous region Download PDF

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CN113229055A
CN113229055A CN202110640237.6A CN202110640237A CN113229055A CN 113229055 A CN113229055 A CN 113229055A CN 202110640237 A CN202110640237 A CN 202110640237A CN 113229055 A CN113229055 A CN 113229055A
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parts
wild
grass
seedlings
pilose antler
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CN113229055B (en
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魏建和
穆滨
王秋玲
翟斌
蒋洁梅
李伟权
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HARBIN KANGLONG PHARMACEUTICAL CO Ltd
Yichun Bingchen Agricultural Technology Development Co ltd
Institute of Medicinal Plant Development of CAMS and PUMC
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HARBIN KANGLONG PHARMACEUTICAL CO Ltd
Yichun Bingchen Agricultural Technology Development Co ltd
Institute of Medicinal Plant Development of CAMS and PUMC
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/40Fabaceae, e.g. beans or peas
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B15/00Organic phosphatic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

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Abstract

The invention provides a wild-simulated cultivation method for a Chinese wild hairy antler grass mountain region, which comprises the steps of firstly cultivating Chinese hairy antler grass cutting seedlings or seedling seedlings symbiotic with hosts as seedlings, selecting an acid soil region with a forest shade coverage degree of less than 60% and a soil layer thickness of more than 30cm in a thinning area of a subtropical forest land to conduct ditching and bedding, transplanting in 3-4 months or 9-10 months, periodically weeding and pruning and keeping moisture, and harvesting after 6-8 months. The method can promote the growth of the pilose antler grass in mountainous forests, reduce weeds, greatly reduce the death rate of the pilose antler grass after transplantation, realize high-quality and high-efficiency wild-simulated cultivation of the pilose antler grass, ensure that the survival rate of the pilose antler grass reaches more than 97.8 percent, ensure that the quality of medicinal materials is consistent with that of the wild, ensure that the content of total flavonoids reaches 5.28 +/-3.86 percent and the content of verbascoside reaches 0.07 +/-0.013 percent, and is suitable for large-scale popularization and application.

Description

Wild-imitating cultivation method for pilose antler grass in mountainous region
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a wild-simulated cultivation method for a pilose antler grass in mountainous regions.
Background
The pilose antler grass Monochama Herba is a common Chinese medicinal material and has the effects of clearing away heat and toxic materials, dispelling wind and relieving pain, and cooling blood and stopping bleeding. The shortage of wild savatier sauropus resource is becoming more and more obvious, which leads to the sharp increase of price, and pharmaceutical enterprises face the risk that the raw materials can not be stably supplied. The adoption of wild-imitating cultivation of traditional Chinese medicinal materials is a good mode for developing under-forest economy in China, but at present, the original plant of the velvet grass, the sansho grass Monochasma saveri Franchet ex Maximowicz has not been reported in documents and patent applications related to the wild-imitating cultivation technology.
The savatier salmoides is used as a hemiparasitic plant, is suitable for growing under shrubs, and a large amount of other shrub crops such as gardenia, tea, oil tea and the like are cultivated in mountainous regions in natural distribution areas of savatier salmoides such as Jiangxi, Fujian, Zhejiang and the like, is suitable for being used as a wild-simulated environment of savatier salmoides, is favorable for improving the economic income of original forest lands, and can also meet the market demand of savatier salmoides. However, the seeds of the sorrel are extremely fine, the early growth of seedlings is extremely slow, the seed consumption is large and the seedling rate is low by adopting a direct seeding mode in forest lands, so that the sorrel is suitable for seedling transplantation; however, in the seedling raising period, the eremophila shanumerous grows lack of hosts, the growth speed is slow after 3 months, the seedlings are required to be transplanted, the seedlings are small and only have 4-8 leaves, the length and width of each leaf is only 1-4 mm, the plant height is only 1-3 cm, the seedlings are transplanted in large-area mountainous regions which are extremely difficult to manage, weeds are difficult to compete, and the seedlings are easy to be buried by rain, so that the survival rate is extremely low.
Disclosure of Invention
In order to solve the technical problem, the invention provides a wild-simulated cultivation method for a Chinese savory mountain land.
The specific technical scheme of the invention is as follows:
the invention provides a wild-imitating cultivation method for a Chinese savory mountain land, which comprises the following steps:
s1: cultivating a cutting seedling of the antlerpilose grass or a seedling symbiotic with the host as a transplanted seedling;
s2: in open forest areas of evergreen broad-leaved forests or needle-wide mixed forests in subtropical regions, selecting an acid soil area with a forest shade coverage degree of less than 60% and a soil layer thickness of more than 30cm, and ditching and making a furrow under the forest, wherein the furrow width is 0.8-1.2 m, the furrow height is 0.25-0.4 m, and the furrow width is 0.5 m;
s3: 3-4 months or 9-10 months per year, when the soil is not completely dried after rain, transplanting the seedlings according to the plant spacing of 20-30 cm and the row spacing of 30-40 cm, wherein the planting depth is 6-7 cm, and covering with fine soil after seeding;
s4: and (4) weeding and pruning regularly, keeping the water content of the furrow surface at 30-40%, and harvesting after 6-8 months.
The method can provide a wild-simulated environment by means of the area under the mountain land forest, effectively promote the growth of the pilose antler grass, obviously reduce the death rate of the transplanted pilose antler grass and obtain the pilose antler grass with the quality close to that of the wild plant.
Further, in step S1, the seedling has a stem height of 2-5 cm, more than 3 branches with stems, and the age of the cutting seedling is more than 2-6 months.
Further, in step S2, the specific point method for ditching and ridging the open space under the forest is as follows:
when the slope of the forest land is greater than 30 degrees, splitting, removing impurities, irrigating and weeds, digging out stones and bamboo roots, and when the slope of the forest land is not greater than 30 degrees, performing land preparation; and then, ditching and ridging along the contour lines, and flowing out of the drainage channel and the operation channel.
Further, the cultivation method of the cutting seedlings comprises the following steps:
(1) cutting the stem branches of the pilose antler grass into small sections, removing leaves on 1-3 stem nodes below each section, and soaking the lower parts of the small sections of stem branches for 30-60 min by using a nutrient solution I; the nutrient solution I comprises the following components in parts by weight:
50-70 parts of urea, 20-30 parts of urea phosphate, 20-30 parts of potassium chloride, 0.3-0.5 part of manganese sulfate, 0.3-0.5 part of ammonium molybdate, 0.3-0.5 part of borax, 10-40 parts of naphthylacetic acid, 10-30 parts of indoleacetic acid and 0.5-1 part of cassava powder;
(2) filling a first culture medium soaked by the nutrient solution I in advance into a culture container, and inserting the soaked small sections of stem branches and the gardenia stem sections with the length of 5-10 cm into the culture medium; the first culture medium is composed of at least one of grass carbon, vermiculite, yellow red loam and river sand;
(3) placing the small sections of the stems at the temperature of more than 20 ℃ for 2-3 weeks, spraying a nutrient solution II every 7 days, and continuing for 20 weeks to obtain finished cutting seedlings; the nutrient solution II comprises the following components in parts by weight:
50-70 parts of urea, 20-40 parts of dipotassium phosphate, 10-30 parts of kinetin, 5-20 parts of naphthylacetic acid, 10-50 parts of 6-benzyladenine, 1-3 parts of brassinolide and 40-70 parts of talcum powder.
The method can effectively improve the survival rate of the stem and branch of the antlerpilose grass in the seedling stage, so that the root system is developed, the overground branches are more, and the stem part is shorter, thereby obtaining stronger cuttage seedlings, and greatly improving the growth vigor and the survival rate after transplanting.
Further, the preparation method of the nutrient solution I comprises the following steps:
weighing all the components in proportion, mixing, dissolving with 300-600 times of sterile double distilled water, adjusting the pH to 5.5-8.0, and storing for later use.
The nutrient solution I can effectively promote the root growth of the small-segment stem branches of the pilose antler grass, promote the overall growth and development of plants, improve the growth condition of the plants and further improve the survival rate in the cutting period.
Further, the first culture medium is composed of the following components in parts by weight:
1-3 parts of turf, 1-7 parts of vermiculite, 1-7 parts of yellow red loam and 1-7 parts of river sand.
The culture medium has sufficient nutrients and proper texture, can provide a required growth environment for the small-segment stems of the antlerpilose grass, and promotes the root pruning of the stems and the growth and development of plants.
Further, the preparation method of the nutrient solution II comprises the following steps:
weighing all the components in proportion, mixing, dissolving with 300-800 times of sterile double distilled water, adjusting the pH to 5.5-8.0, and storing for later use.
The nutrient solution II can effectively promote the growth of overground parts of plants, effectively perform strong seedling and seedling culture, and further improve the survival rate of the plants.
Further, the method for culturing the seedling symbiotic with the host comprises the following steps:
(1) sowing the velvet grass seeds into a second culture medium within 8 months after picking, and carrying out seedling culture in a greenhouse, wherein the second culture medium comprises the following components in parts by weight:
3-5 parts of turf, 0-5 parts of yellow red loam, 0-3 parts of river sand and 0-2 parts of water moss;
(2) transplanting the seedlings into a non-woven fabric seedling raising bag filled with a third culture medium within 1-4 months after emergence of the seedlings, and simultaneously sowing at least one transition host plant in a ratio of 1: 1-5 of host Pirophorum cernum to the host, wherein the transition host plant comprises at least one of gramineae, Labiatae, Compositae, Solanaceae, Brassicaceae, Umbelliferae and Leguminosae; the third culture medium is composed of the following components in parts by weight:
1-2 parts of turf and 3-5 parts of yellow-red soil;
(3) and co-culturing the seedlings and the transition host plants for 1-3 months to obtain strong seedlings suitable for being transplanted to a field or a mountain land.
The method comprises the steps of firstly, germinating and germinating the velvet grass seeds through plug culture in a first stage, then, adding transition host co-culture in a second stage, and reinforcing velvet grass plants by utilizing rapid growth and developed root systems of transition hosts so as to improve the capability of resisting heavy rain and damage after the velvet grass is transplanted outdoors, thereby improving the transplanting survival rate.
Further, the transition host plant is wheat or pepper.
The two hosts are the best hosts aiming at the antlerpilose grass obtained by screening, and can provide the best growth environment for the antlerpilose grass seedlings, so that the stress resistance of the antlerpilose grass is improved to the maximum extent, and the survival rate of transplantation is improved.
Further, the seedling raising conditions are 22-28 ℃, the humidity is 65%, and the illumination is 12h 0% -12h 80%.
The culture condition can provide the temperature and humidity environment most suitable for the emergence of the seeds of the pilose antler grass and sufficient and suitable illumination, thereby greatly improving the germination rate and the germination vigor of the pilose antler grass seeds.
The invention has the following beneficial effects: the invention provides a method for cultivating wild-simulated antlerpilose grass in mountainous regions, which comprises the steps of firstly cultivating antlerpilose grass cutting seedlings or seedlings symbiotic with hosts as seedlings, selecting an acid soil region with a forest shade degree of less than 60% and a soil layer thickness of more than 30cm in a thinning zone of a subtropical forest land to conduct ditching and bedding, transplanting in 3-4 months or 9-10 months, periodically weeding and pruning and keeping moisture, and harvesting after 6-8 months. The method can promote the growth of the pilose antler grass in mountainous forests, reduce weeds, greatly reduce the death rate of the pilose antler grass after transplantation, realize high-quality and high-efficiency wild-simulated cultivation of the pilose antler grass, ensure that the survival rate of the pilose antler grass reaches more than 97.8 percent, ensure that the quality of medicinal materials is consistent with that of the wild, ensure that the content of total flavonoids reaches 5.28 +/-3.86 percent and the content of verbascoside reaches 0.07 +/-0.013 percent, and is suitable for large-scale popularization and application.
Detailed Description
The present invention will be described in further detail with reference to the following examples. It should be noted that the plants or seeds of the velvet antler grass adopted in the following examples and comparative examples are all collected from Lishui city of Zhejiang province, and the experimental sites are all planting bases of the velvet antler grass in Conlong pharmaceutical industry in Yichun city of Jiangxi province.
Example 1
A wild-imitating cultivation method for a Chinese wild deer antler grass mountain land comprises the following steps:
s1: culturing the pilose antler grass cutting seedling by the following method:
(1) collecting the velvet grass, cutting stems with the length of 10-15 cm, cutting into small sections with the length of 2-6 cm, removing leaves on 1-3 stem nodes below each section, and inserting the lower sections into the nutrient solution I to soak for 60 min; the preparation method of the nutrient solution I comprises the following steps:
weighing 5g of urea, 2g of urea phosphate, 2g of potassium chloride, 0.03g of manganese sulfate, 0.03g of ammonium molybdate, 0.03g of borax, 1g of naphthylacetic acid, 1g of indoleacetic acid and 0.05g of cassava powder, mixing, dissolving with 6684g of sterile double distilled water, and adjusting the pH value to 5.5-8.0;
(2) preparing a first culture medium by using turf, vermiculite, yellow-red soil and river sand in a ratio of 1:1:1:1, filling the first culture medium soaked with a nutrient solution I in advance into a non-woven fabric seedling raising bag with the aperture of 2.5cm and the height of 5cm serving as a culture container; inserting the soaked small sections of stems into a culture medium, wherein 1 plant is inserted into each hole, then inserting gardenia stem sections with the length of 5-10 cm, then keeping the gardenia stem sections moist by adopting spray watering, and keeping the environmental temperature above 20 ℃ for 3 weeks;
s3: intermittently spraying nutrient solution II to the small segments of stems and branches every 7 days, culturing for 5 months until the overground stems reach 3-6 strips and grow for 3-8 cm, and filling the containers with fibrous roots in the bags and growing out of the bags to obtain the finished products of the cutting seedlings of the antlerpilose grass; the preparation method of the nutrient solution II comprises the following steps:
5g of urea, 2g of dipotassium phosphate, 1g of kinetin, 0.5g of naphthylacetic acid, 1g of 6-benzyladenine, 0.1g of brassinolide and 4g of talcum powder, mixing, dissolving with 10880g of sterile double distilled water, and adjusting the pH to 5.5-8.0;
s2: in a planting base of corning pharmaceutical industry cornucervi pantotrichum in Yichun city in Jiangxi province, selecting an acid soil area with forest shade shielding degree less than 60% and soil layer thickness more than 30cm under a local secondary forest in spring of 2020, splitting to remove impurities, irrigating and weeds according to the condition of forest land when the gradient is more than 30 degrees, digging out stones and bamboo roots, and performing land preparation when the gradient of the forest land is not more than 30 degrees; then, ditching and making a furrow along the contour line, reserving a drainage channel and an operation channel, wherein the width of the furrow is 0.8-1.2 m, the height of the furrow is 0.25-0.4 m, the width of the furrow is 0.5m, the surface of the furrow is flat, and the surface soil is loose and fine;
s3: transplanting the seedlings according to the planting distance of 20-30 cm and the row distance of 30-40 cm when the soil is not completely dried after rain in 9-10 months, wherein the planting depth is 6-7 cm, and covering with fine soil after transplanting;
s4: intertilling and weeding twice in 2020, trimming dry branches and weak branches with high density in winter, draining water and preventing waterlogging in rainy season, and regularly digging out bamboo shoots which affect growth; and counting the survival rate in 2021 year and 4 months, and detecting the quality of the velvet grass after harvesting.
Example 2
The difference between the method for cultivating the pilose antler grass in the mountain region by imitating the wild cultivation and the embodiment 1 is that the preparation method of the nutrient solution I comprises the following steps:
weighing 7g of urea, 3g of urea phosphate, 3g of potassium chloride, 0.05g of manganese sulfate, 0.05g of ammonium molybdate, 0.05g of borax, 4g of naphthylacetic acid, 3g of indoleacetic acid and 0.1g of cassava powder, mixing, dissolving with 6075g of sterile double distilled water, and adjusting the pH value to 5.5-8.0.
Example 3
The difference between the method and the embodiment 1 is that the preparation method of the nutrient solution II comprises the following steps:
weighing 7g of urea, 4g of dipotassium phosphate, 3g of kinetin, 2g of naphthylacetic acid, 5g of 6-benzyladenine, 3g of brassinolide and 7g of talcum powder, mixing, dissolving by 8490g of sterile double distilled water, and adjusting the pH to 5.5-8.0.
Example 4
A wild-imitating cultivation method for a Chinese wild deer antler grass mountain land comprises the following steps:
s1: culturing the peltate grass seedlings by the following method:
(1) sowing seeds in a plug tray which is filled with a first culture medium and has the aperture of 2-2.5 cm and the height of 3.5-4.5 cm, and placing the plug tray in a greenhouse for seedling culture, wherein the proportion of a second culture medium is turf and yellow-red soil is 5:1, 2-5 seeds are sowed on the surface of the medium in each hole without covering soil, the seedling culture conditions are 22-28 ℃, the humidity is 65%, and the illumination is 12h, 0-12 h and 80%;
(2) transplanting the seedlings into non-woven fabric seedling raising bags which are filled with a second culture medium and have the caliber of 5cm and the height of 10cm 60 days after emergence, and simultaneously sowing two pepper seeds in each bag, wherein the ratio of the third culture medium is turf to yellow and red soil is 1: 3;
(3) culturing young seedlings of the pilose antler grass and the pepper together, watering according to soil moisture, and removing the grass when the grass exists; after the pepper is sowed for 60 days, the pepper is observed to form developed root systems which are fully distributed in a non-woven bag, meanwhile, the overground part of the pilose antler grass reaches 3-4 branches, the stem and branch length is 3-8 cm, and the requirement of transplanting the pilose antler grass into a field or a mountain land can be met;
s2: in a planting base of corning pharmaceutical industry cornucervi pantotrichum in Yichun city in Jiangxi province, selecting an acid soil area with forest shade shielding degree less than 60% and soil layer thickness more than 30cm under a local secondary forest in spring of 2020, splitting to remove impurities, irrigating and weeds according to the condition of forest land when the gradient is more than 30 degrees, digging out stones and bamboo roots, and performing land preparation when the gradient of the forest land is not more than 30 degrees; then, ditching and making a furrow along the contour line, reserving a drainage channel and an operation channel, wherein the width of the furrow is 0.8-1.2 m, the height of the furrow is 0.25-0.4 m, the width of the furrow is 0.5m, the surface of the furrow is flat, and the surface soil is loose and fine;
s3: transplanting the seedlings according to the planting distance of 20-30 cm and the row distance of 30-40 cm when the soil is not completely dried after rain in 9-10 months, wherein the planting depth is 6-7 cm, and covering with fine soil after transplanting;
s4: intertilling and weeding twice in 2020, trimming dry branches and weak branches with high density in winter, draining water and preventing waterlogging in rainy season, and regularly digging out bamboo shoots which affect growth; and counting the survival rate in 2021 year and 4 months, and detecting the quality of the velvet grass after harvesting.
Example 5
A wild-imitating cultivation method for a Chinese wild deer antler grass mountain land comprises the following steps:
s1: culturing the peltate grass seedlings by the following method:
(1) sowing seeds in a plug tray which is filled with a first culture medium and has the aperture of 2-2.5 cm and the height of 3.5-4.5 cm, and placing the plug tray in a greenhouse for seedling culture, wherein the first culture medium comprises turf, yellow and red soil, river sand and water moss which are 3:1:1:1, 2-5 seeds are sowed on the surface of the medium in each plug, no soil is covered above the seeds, the seedling culture conditions are 22-28 ℃, the humidity is 65%, and the illumination is 12h, 0-12 h and 80% switched; the first culture medium is soaked in water in advance, atomized spray watering is adopted after seeding, the soil surface is kept wet all the time before seedling emergence, and water is properly controlled after seedling emergence until the soil is dry and wet;
(2) transplanting the seedlings into a non-woven fabric seedling raising bag which is filled with a second culture medium and has the caliber of 5cm and the height of 10cm 60 days after emergence, wherein the proportion of the second culture medium is turf and yellow red soil is 2: 3; 1 wheat seed is sowed in each bag after 30 days of transplantation;
(3) culturing young seedlings of the pilose antler grass and the wheat together, watering according to soil moisture, and removing grass when the grass exists; after 30 days of wheat seeding, observing that developed root systems are fully distributed in a non-woven bag, and meanwhile, the overground part of the antlerpilose grass reaches 3-6 branches, and the stem and branch length is 4-10 cm, so that the requirement of transplanting the antlerpilose grass into a field or mountain land can be met;
s2: in a planting base of corning pharmaceutical industry cornucervi pantotrichum in Yichun city in Jiangxi province, selecting an acid soil area with forest shade shielding degree less than 60% and soil layer thickness more than 30cm under a local secondary forest in spring of 2020, splitting to remove impurities, irrigating and weeds according to the condition of forest land when the gradient is more than 30 degrees, digging out stones and bamboo roots, and performing land preparation when the gradient of the forest land is not more than 30 degrees; then, ditching and making a furrow along the contour line, reserving a drainage channel and an operation channel, wherein the width of the furrow is 0.8-1.2 m, the height of the furrow is 0.25-0.4 m, the width of the furrow is 0.5m, the surface of the furrow is flat, and the surface soil is loose and fine;
s3: transplanting the seedlings according to the planting distance of 20-30 cm and the row distance of 30-40 cm when the soil is not completely dried after rain in 9-10 months, wherein the planting depth is 6-7 cm, and covering with fine soil after transplanting;
s4: intertilling and weeding twice in 2020, trimming dry branches and weak branches with high density in winter, draining water and preventing waterlogging in rainy season, and regularly digging out bamboo shoots which affect growth; and counting the survival rate in 2021 year and 4 months, and detecting the quality of the velvet grass after harvesting.
Example 6
The mountain wild-imitating cultivation method of the pilose antler grass is different from the embodiment 4 in that the first culture medium comprises turf, yellow red loam and river sand in a ratio of 3:2: 1.
Example 7
The wild-simulated cultivation method of the pilose antler grass in the mountainous region is different from the embodiment 4 in that the ratio of the second culture medium is turf to yellow-red soil in a ratio of 2: 5.
Comparative example 1
A wild-simulated cultivation method for a Chinese alpine rush mountain is different from the embodiment 1 in that stem branches for cuttage are not soaked in a nutrient solution I, and a culture medium is not soaked in the nutrient solution I in advance.
Comparative example 2
The difference between the method for cultivating the pilose antler grass in the mountain region by imitating the wild cultivation and the embodiment 1 is that the preparation method of the nutrient solution I comprises the following steps:
weighing 6g of urea, 2.5g of urea phosphate, 2.5g of potassium chloride, 0.04g of manganese sulfate, 0.04g of ammonium molybdate, 0.04g of borax, 3g of naphthylacetic acid, 2g of indolebutyric acid and 0.08g of cassava powder, mixing, dissolving by 6480g of sterile double distilled water, and adjusting the pH value to 5.5-8.0.
Comparative example 3
The difference between the method for cultivating the pilose antler grass in the mountain region by imitating the wild cultivation and the embodiment 1 is that the preparation method of the nutrient solution I comprises the following steps:
weighing 6g of urea, 2.5g of urea phosphate, 2.5g of potassium chloride, 0.04g of manganese sulfate, 0.04g of ammonium molybdate, 0.04g of borax, 5g of naphthylacetic acid, 1g of indolebutyric acid and 0.08g of cassava powder, mixing, dissolving by 6480g of sterile double distilled water, and adjusting the pH value to 5.5-8.0.
Comparative example 4
A wild-simulated cultivation method of the pilose antler grass in mountainous regions is different from the method in example 1 in that the nutrient solution II is not sprayed.
Comparative example 5
The difference between the method and the embodiment 1 is that the preparation method of the nutrient solution II comprises the following steps:
5g of urea, 2g of dipotassium phosphate, 1g of kinetin, 0.5g of naphthylacetic acid, 1g of 6-benzyladenine, 0.1g of gibberellin and 4g of talcum powder, and after mixing, the mixture is dissolved by 10880g of sterile double distilled water, and the pH is adjusted to 5.5-8.0.
Comparative example 6
The difference between the method and the embodiment 1 is that the preparation method of the nutrient solution II comprises the following steps:
5g of urea, 2g of dipotassium phosphate, 0.5g of naphthylacetic acid, 1g of 6-benzyladenine, 0.1g of brassinolide and 4g of talcum powder, mixing, dissolving by 10880g of sterile double distilled water, and adjusting the pH to 5.5-8.0.
Comparative example 7
A wild-simulated cultivation method for a Chinese velvet antler grass mountain land is different from that of embodiment 4 in that the Chinese velvet antler grass is transplanted after being continuously cultivated for 4 months by a plug cultivation method, transition host co-cultivation is not carried out, intertillage weeding is not carried out after the Chinese velvet antler grass is transplanted to the mountain land, and only the part of the Chinese velvet antler grass, which is more than 30cm away from a big grassland around a transplanted seedling, is cut off.
Comparative example 8
The mountain wild-imitating cultivation method of the savatier monochasma herb is different from the embodiment 1 in that the adopted host is a stem section of the camellia oleifera.
Comparative example 9
A wild-simulated cultivation method of a Chinese wild deer antler grass mountain land is different from the embodiment 1 in that the adopted host is a littleleaf box stem section.
Comparative example 10
A wild-simulated cultivation method of pilose antler grass in mountainous regions is different from embodiment 1 in that ridging is carried out on a flat land field, the width is 1.2m, the height is 0.4m, the groove width is 0.4m, the pilose antler grass transplanting seedling is transplanted into the flat land field, field management is carried out for 3 times of intertillage weeding, and watering is carried out according to the soil moisture; as no miscellaneous trees are on the flat ground, the forest land is not finished and the soil moisture is not opened.
Experimental example 1 comparison of transplanting effect of Antlerpilose grass by different methods
The methods in the above examples and comparative examples are respectively adopted to perform wild-simulated cultivation on the same batch of the same-source eriosema pilosa mountain, and then the survival conditions and the quality of the eriosema pilosa in each group are compared, and the results are shown in table 1.
TABLE 1 comparison of the culture results of different methods
Figure BDA0003106927170000111
Figure BDA0003106927170000121
As can be seen from Table 1, the survival rate of the stem branches of the velvet antler grass in each group of the examples is obviously higher than that of each group of the control group, and the content of the total flavone and the content of the verbascose are equivalent to that of the wild velvet antler grass. The survival rate of the comparative examples 1-6 is obviously lower than that of the examples, and the total flavone content and the verbascose content are integrally obviously lower than that of the wild antlerpilose grass, which shows that when the cutting seedlings are used for transplanting, the use and the specific proportion of the nutrient solution I and the nutrient solution II can influence the growth condition of the cutting seedlings and further influence the survival rate of the transplanted antlerpilose grass. The survival rate, the total flavone content and the verbascose content of the comparison examples 7-9 are also obviously lower than those of the examples and the wild antlerpilose grass, which shows that when the seedlings are transplanted, the selection of hosts can influence the growth condition of the seedlings, and further influence the survival rate and the quality of the transplanted antlerpilose grass; in particular, in comparative example 7, although various weeds are filled in the mountain land and various root systems are filled in the soil and can be used for parasitizing the antlerpilose grass, the survival rate is very low because the antlerpilose grass grows slowly in the early stage and is difficult to resist severe natural environments such as drought, rain wash, weed coverage and the like. In comparative example 10, the environment is relatively superior by adopting a flat land transplantation method, but the indexes of the antleria pilosa are rather reduced compared with the mountain land transplantation, particularly the low temperature resistance is obviously lower, most plants are changed from green to red, and leaves are wilted. Therefore, the survival rate of transplanting the pilose antler grass can be effectively improved by adopting the method provided by the experimental example for cultivation, and the plant with the quality equivalent to that of the wild pilose antler grass can be obtained.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A wild-imitating cultivation method for a Chinese wild deer antler grass mountain land is characterized by comprising the following steps:
s1: cultivating a cutting seedling or a seedling of the antlerpilose grass which is symbiotic with the host as a transplanted seedling;
s2: in open forest areas of evergreen broad-leaved forests or needle-wide mixed forests in subtropical regions, selecting an acid soil area with a forest shade coverage degree of less than 60% and a soil layer thickness of more than 30cm, and ditching and making a furrow under the forest, wherein the furrow width is 0.8-1.2 m, the furrow height is 0.25-0.4 m, and the furrow width is 0.5 m;
s3: 3-4 months or 9-10 months per year, when the soil is not completely dried after rain, transplanting the seedlings according to the plant spacing of 20-30 cm and the row spacing of 30-40 cm, wherein the planting depth is 6-7 cm, and covering with fine soil after transplanting;
s4: and (4) weeding and pruning regularly, keeping the water content of the furrow surface at 30-40%, and harvesting after 6-8 months.
2. The wild-imitating cultivation method for the alpine rush mountain as claimed in claim 1, wherein in step S1, the seedling stem is 2-5 cm high and has more than 3 branches with stem, and the seedling age of the cutting seedling is more than 2-6 months.
3. The method for cultivating the antlerpilose grass in the mountain land imitating wild condition as claimed in claim 1, wherein in step S2, the specific point method for ditching and ridging in the open space under the forest is as follows:
when the slope of the forest land is greater than 30 degrees, splitting, removing impurities, irrigating and weeds, digging out stones and bamboo roots, and when the slope of the forest land is not greater than 30 degrees, performing land preparation; and then, ditching and ridging along the contour lines, and reserving a drainage channel and an operation channel.
4. The wild-simulated cultivation method of the pilose antler grass in the mountainous region as claimed in claim 2, wherein the cultivation method of the cutting seedling is as follows:
(1) cutting the stem branches of the pilose antler grass into small sections, removing leaves on 1-3 stem nodes below each section, and soaking the lower parts of the small sections of stem branches for 30-60 min by using a nutrient solution I; the nutrient solution I comprises the following components in parts by weight:
50-70 parts of urea, 20-30 parts of urea phosphate, 20-30 parts of potassium chloride, 0.3-0.5 part of manganese sulfate, 0.3-0.5 part of ammonium molybdate, 0.3-0.5 part of borax, 10-40 parts of naphthylacetic acid, 10-30 parts of indoleacetic acid and 0.5-1 part of cassava powder;
(2) filling a first culture medium soaked by the nutrient solution I in advance into a culture container, and inserting the soaked small sections of stem branches and the gardenia stem sections with the length of 5-10 cm into the first culture medium; the first culture medium is composed of at least one of grass carbon, vermiculite, yellow red loam and river sand;
(3) placing the small sections of the stems at the temperature of more than 20 ℃ for 2-3 weeks, spraying a nutrient solution II every 7 days, and continuing for 20 weeks to obtain finished cutting seedlings; the nutrient solution II comprises the following components in parts by weight:
50-70 parts of urea, 20-40 parts of dipotassium phosphate, 10-30 parts of kinetin, 5-20 parts of naphthylacetic acid, 10-50 parts of 6-benzyladenine, 1-3 parts of brassinolide and 40-70 parts of talcum powder.
5. The wild-simulated cultivation method of the pilose antler grass in the mountainous region as claimed in claim 4, wherein the preparation method of the nutrient solution I is as follows:
weighing all the components in proportion, mixing, dissolving with 300-600 times of sterile double distilled water, adjusting the pH to 5.5-8.0, and storing for later use.
6. The wild-simulated cultivation method of the antlerpilose grass in the mountainous region as claimed in claim 4, wherein the first culture medium comprises the following components in parts by weight:
1-3 parts of turf, 1-7 parts of vermiculite, 1-7 parts of yellow red loam and 1-7 parts of river sand.
7. The wild-simulated cultivation method of the pilose antler grass in the mountainous region as claimed in claim 4, wherein the preparation method of the nutrient solution II is as follows:
weighing all the components in proportion, mixing, dissolving with 300-800 times of sterile double distilled water, adjusting the pH to 5.5-8.0, and storing for later use.
8. The wild-simulated cultivation method of the antlerpilose grass in the mountainous region as claimed in claim 1, wherein the cultivation method of the seedlings symbiotic with the host is as follows:
(1) sowing the velvet grass seeds into a second culture medium within 8 months after picking, and carrying out seedling culture in a greenhouse, wherein the second culture medium comprises the following components in parts by weight:
3-5 parts of turf, 0-5 parts of yellow red loam, 0-3 parts of river sand and 0-2 parts of water moss;
(2) transplanting the seedlings into a non-woven fabric seedling raising bag filled with a third culture medium within 1-4 months after emergence of the seedlings, and simultaneously sowing at least one transition host plant in a ratio of 1: 1-5 of host Pirophorum cernum to the host, wherein the transition host plant comprises at least one of gramineae, Labiatae, Compositae, Solanaceae, Brassicaceae, Umbelliferae and Leguminosae; the third culture medium is composed of the following components in parts by weight:
1-2 parts of turf and 3-5 parts of yellow-red soil;
(3) and co-culturing the seedlings and the transition host plants for 1-3 months to obtain strong seedlings suitable for being transplanted to a field or a mountain land.
9. The wild-simulated cultivation method of the pilose antler grass in the mountain land of claim 8, wherein the transition host plant is wheat or pepper.
10. The wild-simulated cultivation method for the pilose antler grass in the mountainous region as claimed in claim 9, wherein the seedling raising conditions are 22-28 ℃, the humidity is 65%, and the illumination is switched from 12h to 12h and 80%.
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