CN113197837A

CN113197837A - Folic acid-containing microecological composition and use thereof

Info

Publication number: CN113197837A
Application number: CN202110570106.5A
Authority: CN
Inventors: 郝姗姗; 闫东海; 韩雅慧; 易斌
Original assignee: Jianchangbang Pharmacy Co ltd
Current assignee: Jianchangbang Pharmacy Co ltd
Priority date: 2021-05-25
Filing date: 2021-05-25
Publication date: 2021-08-03

Abstract

The invention discloses a folic acid-containing microecological composition which comprises the following components in parts by weight: 0.01-0.3 part of folic acid and derivatives thereof; 0.09-2.7 parts of soybean fermentation product extract; 0.05-1.5 parts of alpha-glucan oligosaccharide; 0.03-0.9 part of yacon root juice; 0.01-0.3 part of inulin; 0.001-0.03 parts of lactobacillus; 0.001-0.03 part of bifidobacterium. The invention also discloses a cosmetic comprising the folic acid-containing micro-ecological composition.

Description

Folic acid-containing microecological composition and use thereof

Technical Field

The invention relates to the technical field of cosmetics, and particularly relates to a folic acid-containing microecological composition and application thereof.

Background

Acne, also known as acne, is a chronic inflammatory skin disease that manifests itself well in the hair follicles and sebaceous glands of the face, chest and back. The typical development process is as follows: affected by factors such as male hormone and the like, sebum secretion is disordered, and sebaceous gland grease is exuberant in secretion; secondly, the hair follicle is blocked, sebum cannot be normally discharged, a keratotic plug is formed, and acne is generated; bacteria proliferation, especially the increase of the number of propionibacterium acnes, decomposes sebum, releases irritant free fatty acid, initiates inflammatory reaction, and generates pimples and pustules; and fourthly, foreign matters such as inflammatory substances and the like are accumulated continuously, foreign matter sample reaction is generated, deep inflammation around hair follicles is caused, and nodules and cysts are generated. Wherein, skin damage in the fourth stage: papules, pustules, nodules and cysts are inflammatory skin lesions, can be treated by methods of bacteriostasis, anti-inflammation and the like, and have obvious effect; however, acne at the second stage is non-inflammatory skin lesions and is difficult to treat by methods such as bacteriostasis, anti-inflammation and the like. At present, the research and development of acne-removing cosmetics always focuses on the repair of inflammatory skin lesions, acne is often ignored, the acne becomes a state which is common and long-standing on the face of a consumer, and the acne-removing cosmetics are difficult to puzzle for most people.

Aiming at non-inflammatory skin lesions of acne, the current cosmetic nursing measures are mainly to adopt cosmetics containing salicylic acid, fruit acid, azelaic acid, vitamin A acid and the like or to use a guide-out film with strong tearing capacity to strip off cutin, reduce the blockage of hair follicles and normally discharge sebum. However, such cosmetics often cause dry, red, itchy and even allergic skin of consumers, and acne is easy to recur after the use is stopped, thereby treating symptoms and not root causes.

The microecological technology is a new technology for nursing pox at present, and the effects of repairing skin microecological barriers, inhibiting bacteria and resisting inflammation are achieved through external use of prebiotics, prebiotics and probiotics; meanwhile, the advantages of mildly regulating the skin state and enhancing the skin resistance are widely accepted by the market. But the traditional Chinese medicine composition has long generation effect time, has obvious effect on inflammatory skin lesions and has poor effect on non-inflammatory skin lesions and acnes, and is difficult to meet the requirements of most consumers.

Disclosure of Invention

Based on this, aiming at the fact that no product which is practical and effective for non-inflammatory skin lesions and acnes and inflammatory skin lesions, treats both symptoms and root causes, is safe and mild in the traditional technology, a folic acid-containing microecological composition and application thereof are provided.

A folic acid-containing microecological composition comprises the following components in parts by weight:

0.01-0.3 part of folic acid and derivatives thereof;

0.09-2.7 parts of soybean fermentation product extract;

0.05-1.5 parts of alpha-glucan oligosaccharide;

0.03-0.9 part of yacon root juice;

0.01-0.3 part of inulin;

0.001-0.03 parts of lactobacillus;

0.001-0.03 part of bifidobacterium.

In some of these embodiments, the folic acid and derivatives thereof are selected from one or more of folic acid, dihydrofolic acid, tetrahydrofolic acid, 5-formyltetrahydrofolic acid, 10-formyltetrahydrofolic acid, 5, 10-methenyltetrahydrofolic acid, 5, 10-methylenetetrahydrofolic acid, and 5-methyltetrahydrofolic acid.

In some of these embodiments, the soy fermentation product extract is selected from a soy fermentation product extract fermented with bacillus and/or lactobacillus.

In some of these embodiments, the lactobacillus is an inactivated strain selected from one or more of lactobacillus thermophilus, lactobacillus casei, and lactobacillus jensenii.

In some of these embodiments, the bifidobacterium is an inactivated strain selected from one or more of bifidobacterium longum, bifidobacterium breve, bifidobacterium thermophilum, and bifidobacterium ovorans.

In some embodiments, the folic acid-containing microecological composition comprises, by weight, 0.25-0.35 parts of tetrahydrofolic acid, 2.6-2.8 parts of lactobacillus fermented soybean fermentation product extract, 1.4-1.6 parts of alpha-glucan oligosaccharide, 0.8-1.0 part of jerusalem artichoke root juice, 0.25-0.35 part of inulin, 0.025-0.035 part of lactobacillus casei, and 0.025-0.035 part of bifidobacterium breve.

In some embodiments, the folic acid-containing microecological composition comprises, by weight, 0.08-0.12 parts of folic acid, 0.8-1.0 parts of a bacillus fermented soybean fermentation product extract, 0.4-0.6 parts of alpha-glucan oligosaccharide, 0.25-0.35 parts of yacon root juice, 0.08-0.12 parts of inulin, 0.008-0.012 parts of lactobacillus casei and 0.008-0.012 parts of bifidobacterium longum.

In some embodiments, the folic acid-containing micro-ecological composition comprises 0.18-0.22 part of 5, 10-methenyltetrahydrofolic acid, 1.3-1.7 parts of lactobacillus fermented soybean fermentation product extract, 0.8-1.2 parts of alpha-glucan oligosaccharide, 0.5-0.7 part of yacon root juice, 0.18-0.22 part of inulin, 0.018-0.022 part of lactobacillus thermophilus and 0.018-0.022 part of bifidobacterium ovorans.

A cosmetic is cream, emulsion, water, oil, bath product or washing and caring product prepared by adding the above folic acid-containing microecological composition.

In some embodiments, the cosmetic is essence water, and the essence water comprises, by weight, 0.15-0.25 parts of hydroxyethyl cellulose, 0.10-0.20 parts of xanthan gum, 2.5-3.5 parts of methyl propylene glycol, 0.35-0.45 parts of p-hydroxyacetophenone, 0.45-0.55 parts of 1, 2-hexanediol, the folic acid-containing microecological composition, and water.

The invention unexpectedly produces excellent effect of removing noninflammatory skin lesions and acnes by combining folic acid and derivatives thereof, soybean fermentation product extract, alpha-glucan oligosaccharide, yacon root juice, inulin, lactobacillus and bifidobacterium in a reasonable proportion. Can act on the pilosebaceous unit, and has synergistic effect, and can improve the environment of acne, maintain normal metabolism of the pilosebaceous unit, remove noninflammatory acne, and reduce subsequent inflammatory skin injury. Meanwhile, the repair time of inflammatory skin lesions can be greatly shortened, skin barriers can be repaired, and the stable state of skin can be maintained. The invention is safe and has no side effect, can be prepared into cosmetics in various dosage forms, is not limited to face cleaning, water, emulsion, cream, essence, gel, foundation make-up, sun protection and the like, and can be used for daily care of any skin, especially for the care of acne muscles.

Detailed Description

In order that the invention may be more fully understood, reference will now be made to the following description. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

The embodiment of the invention provides a folic acid-containing microecological composition which comprises the following components in parts by weight:

0.01-0.3 part of folic acid and derivatives thereof;

0.09-2.7 parts of soybean fermentation product extract;

0.05-1.5 parts of alpha-glucan oligosaccharide;

0.03-0.9 part of yacon root juice;

0.01-0.3 part of inulin;

0.001-0.03 parts of lactobacillus;

0.001-0.03 part of bifidobacterium.

Folic acid is a complex of vitamin B, and is found in green vegetables and fruits, citrus, yeast, liver, etc. Plays an important role in the one-carbon mechanism of DNA and RNA synthesis, and is involved in many important biochemical processes. Mainly comprises that firstly, the synthesis of purine and thymine is participated in, and DNA and RNA are further synthesized; ② the amino acid is involved in the mutual conversion, and is used as a carrier of a one-carbon unit, such as the interchange of serine and glycine, and the interchange of histidine decomposed into glutamic acid and homocysteine and methionine; ③ the synthesis of hemoglobin and important methyl compounds, such as epinephrine, choline and creatine. The skin, an important component of the whole body of the human body, participates in the metabolism of substances including sugar, fat, water, electrolytes, proteins, etc. The normal metabolism of human skin needs to be maintained by relying on continued epidermal cell proliferation and differentiation. Studies have shown that many skin diseases and even skin cancers are closely related to folate deficiency. It can prevent and repair DNA damage, and is the source of eliminating wrinkles and resisting skin aging.

In the invention, folic acid and derivatives thereof can promote the differentiation, maturation and shedding of keratinocytes of hair follicle parts, reduce the formation of keratotic plugs, improve the condition of hair follicle blockage and maintain the normal metabolism of hair follicle units in hair follicle sebaceous gland units; meanwhile, the skin care product can resist daily skin oxidative damage and maintain normal skin metabolism.

In some embodiments, the folic acid and derivatives thereof are selected from one or more of folic acid, dihydrofolic acid, tetrahydrofolic acid, 5-formyltetrahydrofolic acid, 10-formyltetrahydrofolic acid, 5, 10-methenyltetrahydrofolic acid, 5, 10-methylenetetrahydrofolic acid, and 5-methyltetrahydrofolic acid.

The soybean fermentation product extract belongs to post-production, and is a natural component extracted from metabolites generated by soybeans after being fermented by a specific strain. Is rich in bioactive factors such as plant collagen, small molecular soybean polypeptide, various amino acids, lactic acid, isoflavone, multivitamins, coenzyme Q10, superoxide dismutase (SOD), saponin, etc. Can be selected from soybean fermentation products fermented by bacillus or lactobacillus. The soybean fermentation product extract is a natural component extracted from a metabolite produced by fermenting soybeans with a specific strain. Can enhance skin moisture retention effect, promote collagen activation, and make skin cells full of vitality. The soybean fermentation product extract is commercially available and available from soyama corporation of korea.

In the invention, the soybean fermentation product extract can regulate and control the grease secretion of the sebaceous unit in the pilosebaceous unit and inhibit the release of inflammatory factors; meanwhile, the skin care product provides nutrient substances for the stratum corneum of the skin and moistens the skin.

In some embodiments, the soy fermentation product extract is selected from a soy fermentation product extract fermented with bacillus and/or lactobacillus.

The alpha-glucan oligosaccharide belongs to prebiotics, is available on the market and can be purchased from the company of Xianting in France. Alpha-glucan oligosaccharides are prebiotics obtained by enzymatic synthesis of natural sugars (sucrose and maltose). It is a bioselective substrate that protects and stimulates the skin flora of beneficial microorganisms, but not pathogens and undesirable flora. It can also stimulate keratinocyte to release antibacterial peptide, and has certain moisturizing effect.

In the invention, the alpha-glucan oligosaccharide can regulate and control the beneficial microbial activity of the sebaceous unit in the pilosebaceous unit, reduce the decomposition of pathogenic bacteria on sebum and further reduce the release of inflammatory factors.

The yacon root juice belongs to prebiotics. The yacon root juice is also called yacon or yacon and contains fructose, glucose, sucrose, fructo-oligosaccharide, starch, inulin, a small amount of cellulose, vitamins, mineral elements and the like, wherein the inulin and the fructo-oligosaccharide account for 67 percent of dry matter of the yacon juice, can resist oxidation, eliminate free radicals, have the effects of clearing liver, detoxifying, reducing pathogenic fire and lowering blood pressure, and are effective substances for preventing and treating acne and pimples.

According to the invention, the yacon root juice can selectively promote the colonization of beneficial bacteria such as lactobacillus, bifidobacterium, streptococcus and the like, inhibit the growth of harmful bacteria and improve the flora environment of the pilosebaceous unit; meanwhile, the folic acid can be promoted to be utilized, and the normal metabolism of the follicular unit and the sebaceous gland unit can be maintained.

Inulin belongs to prebiotics. Inulin can be directly used in various cosmetics, and can also be made into fatty acid esters. Inulin can stabilize emulsion, has good dispersibility, spreadability and thickening effect, can inhibit foam, is easy to clean, and has good skin feel; can be used in powder cosmetic for smoothing skin; inulin can be absorbed by skin, and has antioxidant and nutritional effects.

According to the invention, the inulin can protect the skin micro-ecological environment from being damaged by the stimulating components such as preservatives, essence, surfactants and the like in the formula, optimize the micro-ecological barrier and have excellent moisturizing effect; at the same time, oxidative damage to hair follicle keratinocytes can be reduced.

The lactobacillus is a probiotic. Lactobacillus is a generic name for various bacteria that can decompose sugars such as glucose into lactic acid. Lactobacillus is a bacillus-free bacterium, and belongs to gram-positive bacteria. Single, double or short chain arrangement, anaerobic respiration. The lactobacillus has stronger capability of metabolizing carbohydrate to produce acid, can synthesize glucan and heteropolysaccharide, and can ferment saccharide to produce lactic acid or other acid substances. The lactobacillus is widely distributed in the nature, and some strains are one of normal flora of oral cavity, intestinal tract and vagina of human and animals, rarely cause diseases, are basically harmless to human except for causing subacute bacterial endocarditis very occasionally.

In the invention, the lactobacillus is an inactivated strain, and can provide complete effective components of the strain and play the role of active lactobacillus while not influencing the number of microorganisms in cosmetics. The lactobacillus has the effects of promoting the proliferation of beneficial bacteria, keeping the weak acidity of the skin, inhibiting the proliferation of harmful bacteria and improving the balance of the overall skin microecology.

The inactivated probiotics can kill harmful flora, promote the growth of beneficial bacteria, improve the immunity of the skin, repair the skin, consolidate the barrier and achieve the effect of micro-ecological skin care in multiple directions. The target cells for the immune activation of peptidoglycan, polysaccharide and teichoic acid in the inactivated probiotic cell wall components and the components of metabolites (vitamins, free amino acids and the like) of the inactivated probiotic cell wall components are mainly macrophages and B lymphocytes. The inactivated probiotics can activate macrophages to enhance the phagocytic function of the macrophages, and secrete various effector molecules with the function of killing tumors, such as soluble alpha tumor necrosis factor, interleukin 6, interleukin 12, interferon-gamma and the like, wherein the tumor necrosis factor has the effect of killing cancer cells and causes the bleeding and necrosis of tumors. The interleukin is also an important immune active factor, and participates in inflammatory reaction to regulate the body to have immune function. Meanwhile, the inactivated probiotics can also induce B lymphocytes to secrete various antibodies and enhance the killing function of cytotoxic T lymphocytes and natural killer cells.

The lactobacillus is dead bacteria powder obtained by inactivating, centrifuging, extracting, coating and drying lactobacillus bacteria liquid. Wherein, the cell number of the lactobacillus can be: 10⁹～10¹⁴cfu/g。

In some embodiments, the lactobacillus is selected from one or more of lactobacillus thermophilus, lactobacillus casei and lactobacillus jensenii.

The bifidobacteria belong to the group of probiotics. The genus bifidobacterium is a gram-positive, immotile, rod-shaped, sometimes bifurcated, strictly anaerobic genus of bacteria with cells at one end, which is found in the human and animal habitat in the digestive tract, vagina and oral cavity. Bacteria of the genus bifidobacterium are one of the important members of the human and animal intestinal flora.

In the invention, the bifidobacterium is an inactivated strain, and can provide complete effective components of the strain and play the role of active bifidobacterium while not influencing the number of microorganisms in cosmetics. The bifidobacterium can inhibit the growth of harmful bacteria on the surface of the skin, reduce the occurrence of inflammatory reaction and enhance the resistance of the skin; meanwhile, the mild stripping of the hair follicle cutin suppository can be promoted, and the internal environment of the follicular unit is purified.

In some embodiments, the bifidobacterium is an inactivated strain selected from one or more of bifidobacterium longum, bifidobacterium breve, bifidobacterium thermophilum, and bifidobacterium ovorans.

The bifidobacterium is prepared by inactivating, centrifuging, extracting, coating,Drying to obtain dead bacteria powder. Wherein, the number of the somatic cells of the bifidobacterium can be: 10⁹～10¹⁴cfu/g。

In some preferred embodiments, the folic acid-containing microecological composition comprises, by weight, 0.25 to 0.35 parts of tetrahydrofolic acid, 2.6 to 2.8 parts of lactobacillus fermented soybean fermentation product extract, 1.4 to 1.6 parts of alpha-glucan oligosaccharide, 0.8 to 1.0 part of jerusalem artichoke root juice, 0.25 to 0.35 part of inulin, 0.025 to 0.035 parts of lactobacillus casei, and 0.025 to 0.035 parts of bifidobacterium breve.

In some preferred embodiments, the folic acid-containing microecological composition comprises, by weight, 0.08-0.12 parts of folic acid, 0.8-1.0 parts of a bacillus fermented soybean fermentation product extract, 0.4-0.6 parts of alpha-glucan oligosaccharide, 0.25-0.35 parts of yacon root juice, 0.08-0.12 parts of inulin, 0.008-0.012 parts of lactobacillus casei, and 0.008-0.012 parts of bifidobacterium longum.

In some preferred embodiments, the folic acid-containing micro-ecological composition comprises, by weight, 0.18-0.22 parts of 5, 10-methenyltetrahydrofolic acid, 1.3-1.7 parts of lactobacillus fermented soybean fermentation product extract, 0.8-1.2 parts of alpha-glucan oligosaccharide, 0.5-0.7 parts of yacon root juice, 0.18-0.22 parts of inulin, 0.018-0.022 parts of lactobacillus thermophilus, and 0.018-0.022 parts of bifidobacterium ovorans.

The embodiment of the invention also provides a cosmetic which is cream, emulsion, water, oil, bath product or washing product prepared in a general way, and contains the folic acid-containing microecological composition.

In some embodiments, the cosmetic is serum. The essence water comprises, by weight, 0.15-0.25 part of hydroxyethyl cellulose, 0.10-0.20 part of xanthan gum, 2.5-3.5 parts of methyl propylene glycol, 0.35-0.45 part of p-hydroxyacetophenone, 0.45-0.55 part of 1, 2-hexanediol, the folic acid-containing microecological composition and water.

The following are specific examples

Examples 1 to 9

A folic acid-containing microecological composition has the following formula:

example 1

0.01 weight part of folic acid, 0.09 weight part of bacillus fermented soybean fermentation product extract, 0.05 weight part of alpha-glucan oligosaccharide, 0.03 weight part of yacon root juice, 0.01 weight part of inulin, 0.001 weight part of lactobacillus thermophilus and 0.001 weight part of bifidobacterium longum are uniformly mixed at room temperature to prepare the microecological composition 1.

Example 2

Taking 0.3 weight part of tetrahydrofolic acid, 2.7 weight parts of lactobacillus fermented soybean fermentation product extract, 1.5 weight parts of alpha-glucan oligosaccharide, 0.9 weight part of yacon root juice, 0.3 weight part of inulin, 0.03 weight part of lactobacillus casei and 0.03 weight part of bifidobacterium breve, and uniformly mixing at room temperature to prepare the microecological composition 2.

Example 3

Taking 0.05 part by weight of 5-formyl tetrahydrofolic acid, 0.3 part by weight of bacillus fermented soybean fermentation product extract, 0.2 part by weight of alpha-glucan oligosaccharide, 0.1 part by weight of yacon root juice, 0.05 part by weight of inulin, 0.005 part by weight of lactobacillus jensenii and 0.005 part by weight of bifidobacterium thermophilum, and uniformly mixing at room temperature to prepare the microecological composition 3.

Example 4

0.2 part of 5, 10-methenyltetrahydrofolic acid, 1.5 parts of lactobacillus fermented soybean fermentation product extract, 1 part of alpha-glucan oligosaccharide, 0.6 part of yacon root juice, 0.2 part of inulin, 0.02 part of lactobacillus thermophilus and 0.02 part of bifidobacterium ovorans are uniformly mixed at room temperature to prepare the microecological composition 4.

Example 5

0.1 part by weight of folic acid, 0.9 part by weight of bacillus fermented soybean fermentation product extract, 0.5 part by weight of alpha-glucan oligosaccharide, 0.3 part by weight of yacon root juice, 0.1 part by weight of inulin, 0.01 part by weight of lactobacillus casei and 0.01 part by weight of bifidobacterium longum are uniformly mixed at room temperature to prepare the microecological composition 5.

Example 6

Taking 0.3 weight part of tetrahydrofolic acid, 0.09 weight part of lactobacillus fermented soybean fermentation product extract, 0.05 weight part of alpha-glucan oligosaccharide, 0.03 weight part of yacon root juice, 0.01 weight part of inulin, 0.001 weight part of lactobacillus jensenii and 0.001 weight part of bifidobacterium breve, and uniformly mixing at room temperature to prepare the microecological composition 6.

Example 7

0.01 part by weight of 5-formyl tetrahydrofolic acid, 2.7 parts by weight of bacillus fermented soybean fermentation product extract, 0.05 part by weight of alpha-glucan oligosaccharide, 0.03 part by weight of yacon root juice, 0.01 part by weight of inulin, 0.001 part by weight of lactobacillus thermophilus and 0.001 part by weight of bifidobacterium thermophilum are uniformly mixed at room temperature to prepare the microecological composition 7.

Example 8

0.01 part by weight of 5, 10-methenyltetrahydrofolic acid, 0.09 part by weight of lactobacillus fermented soybean fermentation product extract, 1.5 parts by weight of alpha-glucan oligosaccharide, 0.9 part by weight of yacon root juice, 0.3 part by weight of inulin, 0.001 part by weight of lactobacillus casei and 0.001 part by weight of bifidobacterium ovoid are uniformly mixed at room temperature to prepare the microecological composition 8.

Example 9

0.01 part by weight of folic acid, 0.09 part by weight of bacillus fermented soybean fermentation product extract, 0.05 part by weight of alpha-glucan oligosaccharide, 0.03 part by weight of yacon root juice, 0.01 part by weight of inulin, 0.03 part by weight of lactobacillus jensenii and 0.03 part by weight of bifidobacterium longum are uniformly mixed at room temperature to prepare the microecological composition 9.

Comparative examples 1 to 7

Comparative example 1

Comparative example 1 differs from example 5 in that folic acid and its derivatives are replaced with equal parts by weight of vitamin B5. And uniformly mixing at room temperature to prepare the microecological composition 10.

Comparative example 2

Comparative example 2 differs from example 5 in that the soybean fermentation product extract was replaced with an equal weight part of the yeast for secondary fission. And uniformly mixing at room temperature to prepare the microecological composition 11.

Comparative example 3

Comparative example 3 differs from example 5 in that the alpha-glucan oligosaccharides were replaced with equal parts by weight of xylose. And uniformly mixing at room temperature to prepare the microecological composition 12.

Comparative example 4

Comparative example 4 is different from example 5 in that the jerusalem artichoke root juice is replaced with raffinose in an equal weight part. And uniformly mixing at room temperature to obtain the microecological composition 13.

Comparative example 5

Comparative example 5 differs from example 5 in that inulin is replaced with equal parts by weight of dextran. And uniformly mixing at room temperature to prepare the microecological composition 14.

Comparative example 6

Comparative example 6 is different from example 5 in that lactobacillus was replaced with lactobacillus johnsonii in equal parts by weight. And uniformly mixing at room temperature to prepare the microecological composition 15.

Comparative example 7

Comparative example 7 differs from example 5 in that bifidobacteria were replaced with equal parts by weight of Vitreoscilla filiformis. And uniformly mixing at room temperature to prepare the microecological composition 16.

Application examples 1 to 9

The microecological compositions 1 to 9 of examples 1 to 9 were added to serum to obtain acne-removing serum. And testing the safety and the efficacy of the added acne removing essence.

The preparation method of the acne removing essence comprises the following steps:

1. heating water (the total formula is calculated by 100 weight parts, the water amount after removing other components), 0.2 weight part of hydroxyethyl cellulose, 0.15 weight part of xanthan gum, 3 weight parts of methyl propylene glycol, 0.4 weight part of p-hydroxyacetophenone and 0.5 weight part of 1, 2-hexanediol to 80 ℃, and stirring for dissolving;

2. cooling to 45 ℃, respectively adding 1-9 micro-ecological compositions, and stirring for dissolving;

3. and cooling to 35 ℃, defoaming and filtering to obtain application examples 1-9.

Application comparative examples 1 to 7

The microecological compositions 10 to 16 of comparative examples 1 to 7 were added to the essence water to obtain the acne-removing essence water. And testing the safety and the efficacy of the added acne removing essence.

The preparation method of the acne removing essence water comprises the following steps (when the microecological composition does not contain a certain component, the component is not added in the preparation process):

2. cooling to 45 ℃, respectively adding 10-16 micro-ecological compositions, and stirring for dissolving;

3. and cooling to 35 ℃, defoaming and filtering to obtain application comparative examples 1-7.

Application comparative example 8: the commercially available acne-removing essence water containing 2% of salicylic acid (containing no folic acid and its derivatives, soybean fermentation product extract, alpha-glucan oligosaccharide, yacon root juice, inulin, lactobacillus and bifidobacterium in the examples).

Application comparative example 9: the commercially available acne-removing essence containing 2% lactobacillus fermentation product (not containing folic acid and its derivatives, soybean fermentation product extract, alpha-glucan oligosaccharide, yacon root juice, inulin, lactobacillus and bifidobacterium in the examples) specially developed for inflammatory skin lesions.

Safety

Skin occlusion patch test:

510 volunteers meeting the enrollment criteria were selected for the trial and randomized into 17 groups of 30 individuals each.

Inclusion volunteer criteria were: 1) the age is between 15 and 60 years old, and both men and women can use the health care tea; 2) no serious diseases, no immunodeficiency or autoimmune diseases, and no skin treatment and beauty treatment on the tested part; 3) no active allergic disease; 4) the tested part is not applied with any anti-inflammatory drugs in the last two months; 5) one week later with antihistamine or one month later without immunosuppressant. 6) Those who did not perform the patch test within a month.

Exclusion criteria: 1) pregnant or lactating women; 2) patients with severe diseases of heart, lung, cerebral vessels, liver, kidney and hemopoietic system and mental disease; 3) patients suffering from asthma or other chronic respiratory diseases are being treated.

Abort and exit criteria: during the test, the test sample was not applied as required or the record was not recalled as required.

All volunteers need to sign their informed consent. The back of all spot testers is marked with sample numbers, and an equal amount of acne removing essence water is added, a control group is a blank group without any substances, 0.03g of sample is placed in a medicine chamber of a spot test adhesive tape, the spot test adhesive tape with the sample is immediately and longitudinally pasted on normal skin of a left forearm from the lower part, and simultaneously the medicine chamber is lightly pressed one by one to expel air, and the test substances are uniformly distributed. The test site is marked for easy observation. Three groups of parallel lines and a blank group are set, the patch is applied once every 24 hours, and the patch is uncovered for a second half hour, and then the examination is performed according to the grading standard. The patch test was performed 6 times in total, and on day 6 after completion of the skin application, additional examination was performed to observe delayed reactions.

The criteria for the skin irritation test are shown in Table 1. The results are shown in tables 2 and 3.

TABLE 1 grading Standard of adverse skin reactions in the closed Patch test

TABLE 2 skin occlusion Patch test example results

TABLE 3 comparative results of skin-occlusive patch test

The results show that no adverse reaction occurs in human bodies in the application examples 1-9, and the safety is high. As can be seen from comparison between application examples 1-9 and application comparative examples 1-8, the reasonable proportion of the specific components can improve the safety of the product.

(II) evaluation of efficacy of removing noninflammatory skin lesions and acnes

540 volunteers meeting the enrollment criteria were selected for the trial and all volunteers were randomly divided into 18 groups of 30 individuals each. The application examples 1-9, the application comparative examples 1-8 and the blank essence water without the micro-ecological composition are respectively tried on all groups of volunteers.

Inclusion volunteer criteria were: the age is 20-45 years old, and the nature is not limited; people with oily and mixed skin and face with obvious noninflammatory skin damage and acne; hormone drugs and immunosuppressants have not been used in the last month; the tested part does not participate in other clinical testers at present or in nearly 30 days; can be matched with testers well; the system can read and understand all contents of the informed consent and voluntarily sign the informed consent; during the test period, the consent was not to use any cosmetics, drugs and nutraceuticals that have an impact on the outcome.

Exclusion criteria: open wound or erosion face at the acne affected part; those with high allergic constitution; those with a history of cosmetic allergy; a female who is pregnant, lactating, or intended to be pregnant during the test; severe center of gravity, liver and kidney function damage and severe immunologic hypofunction; those with mental disorders, severe endocrine disorders, and oral contraceptives; the patients who participate in the drug clinical testers or other testers within 30 days, or the patients who have systemic drugs which have influence on the test results within about 1 week; those who had oral and topical cosmetic products within 2 weeks that may have an effect on the outcome of the test; the investigator considered not appropriate for attending the investigator.

Abort and exit criteria: smearing the tested sample or recording the revisit according to the requirement during the test period; has serious diseases or serious adverse reactions during the use period.

After the volunteers cleaned the face in the morning and evening every day, the acne removing effect was self-evaluated after continuous use for 4 weeks and 8 weeks using the corresponding acne removing essence water of each group. The evaluation scale was: the effect is obvious, the effect is general, no effect exists, four grades of deterioration appear, and the number of cured people is counted.

The results are shown in Table 4 below.

TABLE 4 therapeutic Effect of non-inflammatory skin lesions on acne

As can be seen from the data in Table 4, after the application examples 1-9 are used, noninflammatory skin lesions and acnes of the volunteers are obviously improved, the cure rate is high, and after the continuous application for 8 weeks, the cure rate reaches 100%. The number of cured patients in examples 2, 4 and 5 after 4 weeks of continuous use can reach more than 20.

As can be seen from comparison between application examples 1-9 and application comparative examples 1-7, any component in the formula of the acne removing cream cannot achieve an excellent acne removing effect, particularly, the acne removing cream is low in cure rate, can only achieve an improved effect, and is difficult to radically cure. It is exactly indicated that the components of the invention can be synergistic and treat both principal and secondary aspect of disease. As can be seen from comparison between application examples 1-9 and application comparative example 8 and blank essence water, the acne cream can reduce conversion from acne to inflammatory skin lesions and maintain normal metabolism of pilosebaceous units.

(III) evaluation of inflammatory skin lesion repair efficacy

540 volunteers meeting the enrollment criteria were selected for the trial and all volunteers were randomly divided into 18 groups of 30 individuals each. Application examples 1-9, application comparative examples 1-7, application comparative example 9 and blank essence water without the micro-ecological composition are respectively tried on all groups of volunteers.

Inclusion volunteer criteria were: the age is 20-45 years old, and the nature is not limited; oily, mixed skin, people with obvious inflammatory lesions, papules, pustules, nodules and cysts on the face; hormone drugs and immunosuppressants have not been used in the last month; the tested part does not participate in other clinical testers at present or in nearly 30 days; can be matched with testers well; the system can read and understand all contents of the informed consent and voluntarily sign the informed consent; during the test period, the consent was not to use any cosmetics, drugs and nutraceuticals that have an impact on the outcome.

After cleaning the face in the morning and evening every day, the volunteers continuously use the corresponding essence water for 28 days. The inflammatory skin lesion repairing efficacy is clinically evaluated by a dermatologist, so that the effective number and the cure number of inflammatory skin lesion repairing are obtained. Evaluation criteria: 1) and (3) curing: disappearance of clinical symptoms, resolution of inflammatory lesions; 2) the method has the following advantages: reduction of clinical symptoms, partial regression or disappearance, reduction or reduction of inflammatory lesions; 3) and (4) invalidation: the clinical symptoms and inflammatory skin lesions are not improved. The number of effective people is recorded on the 1 st day, the 3 rd day, the 7 th day, the 14 th day and the 28 th day after the sample is tried, and the number of cured people is recorded on the 28 th day. The results are shown in Table 5 below.

TABLE 5 inflammatory skin lesion repair efficacy

As can be seen from the data in Table 5, the application examples 1 to 9 have significant efficacy of repairing inflammatory skin lesions, which is obviously superior to the application comparative examples. As can be seen from comparison between the application examples 1-9 and the application comparative examples 1-7 and the application comparative example 9, the components of the invention are combined according to a specific proportion and are matched for use, so that the synergistic effect can be achieved, the inflammatory skin injury repair time can be greatly shortened, and the cure rate is high. The number of cures using example 2, example 4 and example 5 on day 7 can be more than 20.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, so as to understand the technical solutions of the present invention specifically and in detail, but not to be understood as the limitation of the patent protection scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Claims

1. A folic acid-containing microecological composition is characterized by comprising the following components in parts by weight:

0.01-0.3 part of folic acid and derivatives thereof;

0.09-2.7 parts of soybean fermentation product extract;

0.05-1.5 parts of alpha-glucan oligosaccharide;

0.03-0.9 part of yacon root juice;

0.01-0.3 part of inulin;

0.001-0.03 parts of lactobacillus;

0.001-0.03 part of bifidobacterium.

2. The folic acid-containing probiotic composition according to claim 1, characterized in that the folic acid and derivatives thereof are selected from one or more of folic acid, dihydrofolic acid, tetrahydrofolic acid, 5-formyltetrahydrofolic acid, 10-formyltetrahydrofolic acid, 5, 10-methenyltetrahydrofolic acid, 5, 10-methylenetetrahydrofolic acid and 5-methyltetrahydrofolic acid.

3. The folate-containing probiotic composition according to claim 1, wherein said soy fermentation product extract is selected from the group consisting of a soy fermentation product extract fermented with bacillus and/or lactobacillus.

4. The folic acid-containing microecological composition according to claim 1, wherein the lactic acid bacteria is an inactivated strain selected from one or more of Lactobacillus thermophilus, Lactobacillus casei and Lactobacillus jensenii.

5. The folic acid-containing probiotic composition according to claim 1, characterized in that said bifidobacteria are inactivated strains selected from one or more of the group consisting of bifidobacterium longum, bifidobacterium breve, bifidobacterium thermophilum and bifidobacterium ovorans.

6. The folic acid-containing microecological composition according to claim 1, which comprises 0.25 to 0.35 parts by weight of tetrahydrofolic acid, 2.6 to 2.8 parts by weight of a lactobacillus fermented soybean fermentation product extract, 1.4 to 1.6 parts by weight of alpha-glucan oligosaccharide, 0.8 to 1.0 part by weight of yacon root juice, 0.25 to 0.35 part by weight of inulin, 0.025 to 0.035 part by weight of lactobacillus casei, and 0.025 to 0.035 part by weight of bifidobacterium breve.

7. The folic acid-containing microecological composition according to claim 1, which comprises, by weight, 0.08 to 0.12 parts of folic acid, 0.8 to 1.0 part of an extract of a fermented soybean product of bacillus, 0.4 to 0.6 part of an alpha-glucan oligosaccharide, 0.25 to 0.35 part of yacon root juice, 0.08 to 0.12 part of inulin, 0.008 to 0.012 parts of lactobacillus casei, and 0.008 to 0.012 parts of bifidobacterium longum.

8. The folic acid-containing microecological composition according to claim 1, which comprises 0.18 to 0.22 part by weight of 5, 10-methenyltetrahydrofolic acid, 1.3 to 1.7 parts by weight of a lactobacillus fermented soybean fermentation product extract, 0.8 to 1.2 parts by weight of alpha-glucan oligosaccharide, 0.5 to 0.7 part by weight of yacon root juice, 0.18 to 0.22 part by weight of inulin, 0.018 to 0.022 part by weight of lactobacillus thermophilus, and 0.018 to 0.022 part by weight of bifidobacterium ovorans.

9. Cosmetic products, characterized in that they are creams, emulsions, water, oils, bathing or washing products, prepared in a versatile manner, containing a folic acid containing microecological composition according to any one of claims 1 to 8.

10. The cosmetic according to claim 9, wherein the cosmetic is essence water, and the essence water comprises, by weight, 0.15-0.25 parts of hydroxyethyl cellulose, 0.10-0.20 parts of xanthan gum, 2.5-3.5 parts of methyl propylene glycol, 0.35-0.45 parts of p-hydroxyacetophenone, 0.45-0.55 parts of 1, 2-hexanediol, the folic acid-containing microecological composition, and water.