CN111374896A

CN111374896A - Prebiotics and probiotic skin care product for treating acne and preparation method thereof

Info

Publication number: CN111374896A
Application number: CN201811625588.4A
Authority: CN
Inventors: 王英; 刘凤岩; 杨晓蕾; 李丹
Original assignee: Xi'an Zhenrui Biological Technology Co ltd
Current assignee: Xi'an Zhenrui Biological Technology Co ltd
Priority date: 2018-12-28
Filing date: 2018-12-28
Publication date: 2020-07-07

Abstract

The invention discloses a prebiotics and probiotic skin care product for treating acne and a preparation method thereof, wherein the skin care product comprises the following components in percentage by mass: 1-4% of polyoxyethylene 100 stearate, 1-10% of prebiotics, 1-10% of probiotics, 1-5% of caprylic/capric triglyceride, 1-4% of stearic acid, 2-6% of glycerol, 0.01-0.2% of sodium hyaluronate, 0.1-2% of phytosterol, 0.1-2% of cetearyl alcohol, 0.1-3% of isononyl isononanoate, 1-10% of plant extract, 0.5-3% of hydroxyethyl cellulose, 0.1-0.5% of phenoxyethanol and the balance of water. The invention has simple formula, convenient use, good effect and no toxic or side effect.

Description

Prebiotics and probiotic skin care product for treating acne and preparation method thereof

Technical Field

The invention relates to the technical field of skin care products, in particular to a prebiotics and probiotic skin care product for treating acne and a preparation method thereof.

Background

The skin is the organ with the largest surface area of the human body and also the first defense line of the body for defending the invasion of harmful substances in the external environment, besides the physical barrier formed by keratinocytes, the skin can also secrete a plurality of substances such as antibacterial peptide, active oxygen, free fatty acid and the like, plays a role in directly killing pathogenic microorganisms invading the epidermis of the skin, and is an important component of the innate immunity of the human body. In addition, there have been long-term related studies that indicate that skin serves as a bioactive interface between the internal and external environments of the human body, and the surface of the skin inhabits a large number of microorganisms, including bacteria, fungi, and the like. These microorganisms form different niches on the skin surface, forming a complex ecosystem, the skin micro-ecology. Most microorganisms on the skin surface are harmless to the human body, and even some microorganisms are beneficial. The normal symbiotic flora of the skin can regulate and control inflammatory reaction after the skin is damaged and participate in local immune defense, and can inhibit the propagation of pathogenic microorganisms by directly secreting or inducing the generation of the antibacterial peptide of the body. However, when the balance relationship between the symbiotic bacteria on the skin surface is broken, the function of the skin is disturbed and diseases occur. Many studies have also demonstrated that the onset of a variety of inflammatory skin diseases such as acne, atopic dermatitis, and the delayed healing of chronic wounds are associated with disturbances in the skin flora.

Acne is commonly known as "whelk", "comedo" or "pimple", and is a chronic inflammatory skin disease of hair follicle sebaceous glands, which is mainly developed in adolescents and can also be seen in children and adults. The affected parts are mainly distributed on the parts with vigorous sebaceous gland secretion, such as the face, the neck, the chest and the back. The clinical manifestations are acne, papule, pustule, nodule and cyst. The disease is strongly related to the primary dysbiosis of the skin caused by the massive proliferation of propionibacterium acnes. Propionibacterium acnes mainly induces immune reactions and local inflammatory reactions of the body during the attack of acne. Acne treatment methods are various, and at present, medicaments such as vitamin A acids, antibiotics, antiandrogens and glucocorticoids are mainly used through oral administration or local external application. The methods have the advantages of long treatment period, large skin irritation caused by incomplete treatment, easy generation of drug resistance, easy recurrence of acne, large side effect and skin injury.

Probiotics are a class of active microorganisms that are beneficial in maintaining or enhancing a beneficial balance of microbial flora in the body. Typical probiotics are lactobacilli, including bifidobacteria, lactic acid bacteria, and the like. Prebiotics are non-digestible fermentable carbohydrates capable of selectively stimulating probiotics, and mainly comprise fructo-oligosaccharide, glucan, resistant starch and the like. Probiotics are commonly used as food and pharmaceuticals to maintain gastrointestinal microflora balance. In recent years, with intensive research on probiotics, it has been found that probiotics can be applied to the skin in addition to the skin, and thus have great potential in the aspects of protecting healthy skin, preventing and treating skin diseases, and the like. Studies have shown that an acidic skin environment can prevent bacterial colonization, while the probiotic microflora can provide a moist acidic barrier environment for the skin by fermenting metabolic acidic molecules (such as lactic acid), amino acids and other acidic substances. In addition, studies have shown that topical application to the skin, with probiotic, spore, and extracellular products as the major components, can prevent the growth of bacteria, fungi, viruses, and other compositions, effectively maintaining the normal function of healthy skin. Prebiotics can beneficially affect the host by selectively stimulating the growth and activity of one or a small number of probiotic bacteria to improve host health. Researches show that the prebiotics can help to maintain the natural protective barrier on the surface of the skin, increase the resistance of the skin to harmful bacteria, maintain the natural balance state of the skin and reduce the generation of skin problems.

With the rise of skin micro-ecology, a green ecological preparation is developed by utilizing the modern biotechnology to replace the traditional medicines such as antibiotics, hormones and the like, the steady state of healthy skin flora is recovered and maintained through probiotic intervention effectively, the aim of treating skin diseases is finally achieved, the micro-ecological balance of the skin is fundamentally adjusted and maintained, so that the skin problem is solved, and the preparation method becomes a new research direction in the field of skin care products/cosmetics.

In order to solve the defects of long treatment period, incomplete treatment, easy repetition, large side effect, easy formation of drug resistance, low total cure rate and the like of the existing facial acne treatment products, the invention provides a prebiotic and probiotic skin care product for treating facial acne. The skin care product containing the prebiotics and the probiotics provided by the invention takes the prebiotics and the probiotics as functional components, is supplemented with other effective components, has a remarkable effect of treating facial acne by the synergistic effect of the components, can improve the micro-ecology on the surface of the skin of a human body, and does not contain antibiotics and hormone components, and has mild effect, no skin irritation and high safety.

Disclosure of Invention

Aiming at the defects in the prior art, the prebiotics and probiotic bacteria skin care product provided by the invention takes the prebiotics and the probiotic bacteria as functional components, is supplemented with other effective components, has a synergistic effect among the components, can improve the micro-ecology on the surface of human skin, and has an obvious effect of treating facial acne.

In order to achieve the purpose, the invention provides the following technical scheme:

a prebiotic and probiotic skin care product for treating acne comprises the following components in percentage by mass: 1-4% of polyoxyethylene 100 stearate, 1-10% of prebiotics, 1-10% of probiotics, 1-5% of caprylic/capric triglyceride, 1-4% of stearic acid, 2-6% of glycerol, 0.01-0.2% of sodium hyaluronate, 0.1-2% of phytosterol, 0.1-2% of cetearyl alcohol, 0.1-3% of isononyl isononanoate, 1-10% of plant extract, 0.5-3% of hydroxyethyl cellulose, 0.1-0.5% of phenoxyethanol and the balance of water.

As a still further scheme of the invention: the prebiotics and probiotic skin care product for treating acne comprises the following components in percentage by mass: 2-3% of polyoxyethylene 100 stearate, 3-5% of prebiotics, 3-5% of probiotics, 2-4% of caprylic/capric triglyceride, 2-3% of stearic acid, 3-5% of glycerol, 0.01-0.1% of sodium hyaluronate, 0.5-1% of phytosterol, 0.5-1% of whale wax stearyl alcohol, 0.5-2% of isononyl isononanoate, 1-6% of plant extract, 0.5-3% of hydroxyethyl cellulose, 0.2-0.3% of phenoxyethanol and the balance of water.

The invention further adopts the scheme that the prebiotics are oat β glucan, fructo oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose, and the weight ratio of the prebiotics to the prebiotics is 2 (1-10).

As a still further scheme of the invention: the probiotics are bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri, and the mass ratio is 3: (1-10): (1-10): (1-10): (1-10): (1-10).

As a still further scheme of the invention: the plant extract is a horse chestnut extract, a wild soybean extract, a salvia miltiorrhiza root extract, a centella asiatica extract and a phellodendron amurense extract, and the weight ratio of the plant extract to the plant extract is 1: (1-10): (1-10): (1-10): (1-10).

As a still further scheme of the invention: the prebiotics and probiotic skin care product for treating acne comprises the following components in percentage by mass: 2% polyoxyethylene 100 stearate, 2.5% caprylic capric triglyceride, 3% stearic acid, 0.5% phytosterol, 1% cetearyl alcohol, 0.5% isononyl isononanoate, 5% prebiotics, 3% probiotics, 3% glycerol, 0.06% sodium hyaluronate, 1.5% hydroxyethyl cellulose, 5% plant extract, 0.25% phenoxyethanol, 72.69% water;

wherein the prebiotics comprise oat β glucan, fructo-oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose, and the mass ratio is 2: 3: 5: 1: 2: 1;

the probiotics comprise bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri in a mass ratio of 3: 2: 4: 2: 2: 1;

the plant extract comprises a aesculus hippocastanum extract, a wild soybean extract, a salvia miltiorrhiza root extract, a centella asiatica extract and a phellodendron amurense extract, and the mass ratio of the extracts is 1: 2: 4: 3: 1.

as a still further scheme of the invention: the preparation method of the prebiotics and probiotic skin care product for treating acne comprises the following steps:

(1) heating and stirring polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetostearyl alcohol and isononyl isononanoate according to a proportion until the materials are completely dissolved, and stirring for 25-30 min at a constant temperature of 75-80 ℃ to obtain a material A for later use;

(2) adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water according to a proportion, heating and stirring until the prebiotics, the probiotics, the glycerol, the sodium hyaluronate and the hydroxyethyl cellulose are completely dissolved, and stirring for 25-30 min at a constant temperature of 75-80 ℃ to obtain a material B for later use;

(3) mixing the plant extract and phenoxyethanol according to a proportion, and uniformly stirring to obtain a material C for later use;

(4) adding the material B into the material A, adding into an emulsifying pot, and homogenizing at 75-80 ℃ for 3-5 min;

(5) and (3) when the temperature of the materials in the emulsifying pot is reduced to below 45 ℃, adding the material C, uniformly stirring and then discharging.

The skin care product containing the prebiotics and the probiotics provided by the invention takes oat β glucan, fructo-oligosaccharide, α glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose as probiotics, takes bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri as probiotics, combines other effective components, can promote the proliferation of normal flora types of staphylococcus epidermidis, can inhibit the growth of potential pathogenic bacteria such as staphylococcus aureus, propionibacterium acnes and the like on the surface of the skin, and obviously regulates the flora balance in facial acne skin, wherein fatty acids such as lactic acid, acetic acid, butyric acid and the like generated by lactobacillus plantarum and lactobacillus reuteri can reduce the pH value of the skin, inhibit the growth and propagation of harmful bacteria, and have a protective effect on the skin.

The prebiotic and probiotic skin care product provided by the invention can contain plant extracts, including aesculus hippocastanum extract, wild soybean extract, salvia miltiorrhiza root extract, centella asiatica extract and phellodendron amurense extract, can inhibit the activity of 5 α reductase by inhibiting the conversion of testosterone into dihydrotestosterone, reduce the hyperfunction of sebaceous glands, balance sebum secretion, dredge pores, improve the keratinization of hair follicles, and improve the oily and acne-prone skin conditions.

In vitro experiments show that the composition can effectively promote the proliferation of normal flora types of staphylococcus epidermidis, and has obvious inhibiting effect on staphylococcus aureus and propionibacterium acnes which are acne pathogenic bacteria.

Clinical experiments show that the composition has a remarkable treatment effect on facial acne, the total effective rate is 96.2%, acne disappears after a subject continuously uses the composition, skin damage subsides, and damaged skin barriers are quickly and effectively repaired. And no side effects such as skin allergy, dryness, stabbing pain, inflammation and the like occur.

Drawings

FIG. 1 is a comparison graph of a patient with facial acne after one cycle of treatment with a composition of the present invention (comparison graph before and after treatment of a patient with facial acne).

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it should be apparent that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.

Example 1:

the formula is as follows: polyoxyethylene 100 stearate 2%, prebiotics 3%, probiotics 5%, caprylic capric triglyceride 2%, stearic acid 2%, glycerin 5%, sodium hyaluronate 0.05%, phytosterol 1%, cetearyl alcohol 0.5%, isononyl isononanoate 2%, plant extract 6%, hydroxyethyl cellulose 1%, phenoxyethanol 0.3%, and water 70.15%.

The prebiotics of the embodiment comprise oat β glucan, fructo-oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose in a mass ratio of 2: 4: 3: 2: 1: 1.

The probiotics of the embodiment comprise bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri in a mass ratio of 3: 3: 5: 2: 1: 2.

the plant extracts of the embodiment comprise aesculus hippocastanum extract, wild soybean extract, salvia miltiorrhiza root extract, centella asiatica extract and phellodendron amurense extract according to the mass ratio of 1: 3: 5: 2: 1.

the preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetostearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring for 25-30 min at constant temperature of 75-80 ℃ to obtain a material A for later use. And adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until the prebiotics, the probiotics, the glycerol, the sodium hyaluronate and the hydroxyethyl cellulose are completely dissolved, and stirring for 25-30 min at a constant temperature of 75-80 ℃ to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. And adding the material B into the material A at the temperature of 75-80 ℃, and adding into an emulsifying pot for homogenizing for 3-5 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 40 ℃, uniformly stirring and then discharging.

Example 2:

this example was formulated as in example 1, but the temperature and time of the preparation were varied. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring at constant temperature of 78 ℃ for 27min to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at constant temperature of 77 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 78 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 41 ℃, uniformly stirring and discharging.

Example 3:

the formula is as follows: polyoxyethylene 100 stearate 2%, prebiotics 5%, probiotics 3%, caprylic capric triglyceride 2.5%, stearic acid 3%, glycerin 3%, sodium hyaluronate 0.06%, phytosterol 0.5%, cetearyl alcohol 1%, isononyl isononanoate 0.5%, plant extract 5%, hydroxyethyl cellulose 1.5%, phenoxyethanol 0.25%, water 72.69%.

The prebiotics of the embodiment comprise oat β glucan, fructo-oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose in a mass ratio of 2: 3: 5: 1: 2: 1.

The probiotics of the embodiment comprise bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri in a mass ratio of 3: 2: 4: 2: 2: 1.

the plant extracts of the embodiment comprise aesculus hippocastanum extract, wild soybean extract, salvia miltiorrhiza root extract, centella asiatica extract and phellodendron amurense extract according to the mass ratio of 1: 2: 4: 3: 1.

Example 4:

this example was formulated as in example 3, but the temperature and time of the preparation were varied. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring at the constant temperature of 77 ℃ for 28min to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at the constant temperature of 78 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 78 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 43 ℃, uniformly stirring and then discharging.

Example 5:

the formula is as follows: polyoxyethylene 100 stearate 1.5%, prebiotics 4%, probiotics 4%, caprylic capric triglyceride 3%, stearic acid 2.5%, glycerin 4%, sodium hyaluronate 0.1%, phytosterol 1.5%, cetostearyl alcohol 0.8%, isononyl isononanoate 1%, plant extract 4%, hydroxyethyl cellulose 0.8%, phenoxyethanol 0.2%, water 72.6%.

The prebiotics of the embodiment comprise oat β glucan, fructo-oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose in a mass ratio of 2: 3: 3: 1: 2: 2.

The probiotics of the embodiment comprise bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri in a mass ratio of 3: 4: 3: 3: 1: 7.

the plant extracts of the embodiment comprise aesculus hippocastanum extract, wild soybean extract, salvia miltiorrhiza root extract, centella asiatica extract and phellodendron amurense extract according to the mass ratio of 1: 4: 3: 1: 3.

Example 6:

this example was formulated as in example 5, but the temperature and time of the preparation were varied. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring for 28min at constant temperature of 78 ℃ to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at the constant temperature of 78 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 77 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 42 ℃, uniformly stirring and discharging.

Example 7:

the formula is as follows: 1% of polyoxyethylene 100 stearate, 1% of prebiotics, 1% of probiotics, 1% of caprylic/capric triglyceride, 1% of stearic acid, 2% of glycerol, 0.01% of sodium hyaluronate, 0.1% of phytosterol, 0.1% of cetearyl alcohol, 0.1% of isononyl isononanoate, 1% of plant extract, 0.5% of hydroxyethyl cellulose, 0.1% of phenoxyethanol and 91.09% of water.

The prebiotics of the embodiment comprise oat β glucan, fructo-oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose in a mass ratio of 2: 1: 1: 1: 1: 1.

The probiotics of the embodiment comprise bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri in a mass ratio of 3: 1: 1: 1: 1: 1.

the plant extracts of the embodiment comprise aesculus hippocastanum extract, wild soybean extract, salvia miltiorrhiza root extract, centella asiatica extract and phellodendron amurense extract according to the mass ratio of 1: 1: 1: 1: 1.

Example 8:

this example was formulated identically to example 7, but with different temperatures and times in the preparation process. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring for 28min at constant temperature of 78 ℃ to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at the constant temperature of 78 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 77 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 42 ℃, uniformly stirring and discharging.

Example 9:

the formula is as follows: 4% of polyoxyethylene 100 stearate, 10% of prebiotics, 10% of probiotics, 5% of caprylic/capric triglyceride, 4% of stearic acid, 6% of glycerol, 0.2% of sodium hyaluronate, 2% of phytosterol, 2% of cetearyl alcohol, 3% of isononyl isononanoate, 10% of plant extract, 3% of hydroxyethyl cellulose, 0.5% of phenoxyethanol and 40.3% of water.

The prebiotics of the embodiment comprise β oat glucan, fructo-oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose in a mass ratio of 2: 10: 10: 10: 10: 10.

The probiotics of the embodiment comprise bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri in a mass ratio of 3: 10: 10: 10: 10: 10.

the plant extracts of the embodiment comprise aesculus hippocastanum extract, wild soybean extract, salvia miltiorrhiza root extract, centella asiatica extract and phellodendron amurense extract according to the mass ratio of 1: 10: 10: 10: 10.

Example 10:

this example was formulated as in example 9, but the temperature and time were varied during the preparation. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring for 28min at constant temperature of 78 ℃ to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at the constant temperature of 78 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 77 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 42 ℃, uniformly stirring and discharging.

Example 11:

the formula is as follows: polyoxyethylene 100 stearate 2%, prebiotics 5%, probiotics 5%, caprylic capric triglyceride 3%, stearic acid 2%, glycerin 4%, sodium hyaluronate 0.1%, phytosterol 1%, cetearyl alcohol 1%, isononyl isononanoate 1.5%, plant extract 6%, hydroxyethyl cellulose 1.7%, phenoxyethanol 0.3%, and water 67.4%.

The prebiotics of the embodiment comprise oat β glucan, fructo-oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose in a mass ratio of 2: 5: 5: 5: 5: 5.

The probiotics of the embodiment comprise bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri in a mass ratio of 3: 5: 5: 5: 5: 5.

the plant extracts of the embodiment comprise aesculus hippocastanum extract, wild soybean extract, salvia miltiorrhiza root extract, centella asiatica extract and phellodendron amurense extract according to the mass ratio of 1: 5: 5: 5: 5.

Example 12:

this example was formulated identically to example 11, but with a different temperature and time of preparation. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring at the constant temperature of 78 ℃ for 28min to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at the constant temperature of 78 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 77 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 42 ℃, uniformly stirring and discharging.

Example 13:

the formula is as follows: polyoxyethylene 100 stearate 2%, prebiotics 3%, probiotics 3%, caprylic capric triglyceride 2%, stearic acid 2%, glycerin 3%, sodium hyaluronate 0.01%, phytosterol 0.5%, cetostearyl alcohol 0.5%, isononyl isononanoate 0.5%, plant extract 1%, hydroxyethyl cellulose 0.5%, phenoxyethanol 0.2%, water 81.79%.

Example 14:

this example was formulated identically to example 13, but with a different temperature and time of preparation. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring at the constant temperature of 78 ℃ for 28min to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at the constant temperature of 78 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 77 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 42 ℃, uniformly stirring and discharging.

Example 15:

the formula is as follows: 3% of polyoxyethylene 100 stearate, 5% of prebiotics, 5% of probiotics, 4% of caprylic/capric triglyceride, 3% of stearic acid, 5% of glycerol, 0.1% of sodium hyaluronate, 1% of phytosterol, 1% of cetearyl alcohol, 2% of isononyl isononanoate, 6% of plant extract, 3% of hydroxyethyl cellulose, 0.3% of phenoxyethanol and 61.6% of water.

Example 16:

this example was formulated identically to example 15, but with different temperatures and times in the preparation process. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring at the constant temperature of 78 ℃ for 28min to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at the constant temperature of 78 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 77 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 42 ℃, uniformly stirring and discharging.

Example 17:

the formula is as follows: polyoxyethylene 100 stearate 2.5%, prebiotics 3.5%, probiotics 3.5%, caprylic capric triglyceride 3%, stearic acid 2.5%, glycerin 3.5%, sodium hyaluronate 0.05%, phytosterol 0.7%, cetearyl alcohol 0.8%, isononyl isononanoate 1.2%, plant extract 3.5%, hydroxyethyl cellulose 1.8%, phenoxyethanol 0.25%, and water 73.2%.

Example 18:

this example was formulated identically to example 17, but with different temperatures and times. The preparation method comprises the following steps: weighing all the raw materials according to mass percentage, taking polyoxyethylene 100 stearate, caprylic capric triglyceride, stearic acid, phytosterol, cetearyl alcohol and isononyl isononanoate, heating and stirring until the raw materials are completely dissolved, and stirring at the constant temperature of 78 ℃ for 28min to obtain a material A for later use. Adding prebiotics, probiotics, glycerol, sodium hyaluronate and hydroxyethyl cellulose into water, heating and stirring until completely dissolved, and stirring at the constant temperature of 78 ℃ for 27min to obtain a material B for later use. Mixing the plant extract and phenoxyethanol, and stirring to obtain material C. Adding the material B into the material A at 77 ℃, and adding into an emulsifying pot for homogenizing for 4 min. And (3) adding the material C when the temperature of the material in the emulsifying pot is reduced to 42 ℃, uniformly stirring and discharging.

In vitro experiments

The compositions of examples 1-18 were applied to skin microorganisms cultured in vitro and their regulatory effects on skin microorganisms were verified.

The test method is as follows:

(1) respectively inoculating Staphylococcus epidermidis (ATCC49134 standard strain) and Staphylococcus aureus (ATCC6538 standard strain) to YPDA liquid culture medium, and inoculating Propionibacterium acnes (ATCC6919 standard strain) to GAM broth culture medium.

(2) 20mL of each culture broth, 19 aliquots of each culture broth, were added with 0.2mL of the composition of examples 1-18 and 0.2mL of deionized water (control). Then putting staphylococcus epidermidis and staphylococcus aureus in a 37 ℃ incubator, culturing in an aerobic environment for 24 hours, putting propionibacterium acnes in the 37 ℃ incubator, and culturing in an anaerobic environment for 48 hours.

(3) The number of each bacterium in the culture solution before and after the culture was measured by the dilution plate method. The measurement results are shown in table 1.

TABLE 1 comparison of skin microbial counts before and after incubation (cfu/ml)

The experimental results prove that the composition can effectively promote the increment of the normal flora type of the staphylococcus epidermidis, and has obvious inhibiting effect on acne pathogenic bacteria staphylococcus aureus and propionibacterium acnes.

Clinical trial

The facial acne treatment comprises 80 facial acne patients, wherein 23 men and 57 women are in the group, the person with the largest age is 38 years old, the person with the smallest age is 16 years old, the average age is 21.8 years old, and all cases meet the acne diagnosis standard in the traditional Chinese medicine disease diagnosis curative effect standard (2012). During the test period, the patient cleans the face every morning and evening and then applies the composition, stops using other cosmetics and medicines, and keeps the light diet and regular work and rest, and the test period is 4 weeks. Recording the number, skin lesion degree and red and swollen conditions of facial acnes of the testee before and after the test; and the skin condition of each group of subjects was observed and the post-use experience of the subjects was revisited.

After 4 weeks of trial, most of 80 patients (56 patients) had acne disappeared, skin lesions resolved, and subjective symptoms disappeared; acne is reduced for 21 people, subjective symptoms are obviously relieved, and skin lesions are faded by more than 30 percent; 3 the symptoms of the people are not improved; the total cure rate is 70 percent, and the effective rate is 96.2 percent. In all cases, other adverse reactions such as skin allergy, dryness, pricking pain, inflammation and the like do not occur in the treatment process.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention should be covered by the scope of the present invention.

Claims

1. A prebiotics and probiotic skin care product for treating acne is characterized by comprising the following components in percentage by mass: 1-4% of polyoxyethylene 100 stearate, 1-10% of prebiotics, 1-10% of probiotics, 1-5% of caprylic/capric triglyceride, 1-4% of stearic acid, 2-6% of glycerol, 0.01-0.2% of sodium hyaluronate, 0.1-2% of phytosterol, 0.1-2% of cetearyl alcohol, 0.1-3% of isononyl isononanoate, 1-10% of plant extract, 0.5-3% of hydroxyethyl cellulose, 0.1-0.5% of phenoxyethanol and the balance of water.

2. The prebiotic and probiotic skin care product for the treatment of acne according to claim 1 comprising the following components in mass percent: 2-3% of polyoxyethylene 100 stearate, 3-5% of prebiotics, 3-5% of probiotics, 2-4% of caprylic/capric triglyceride, 2-3% of stearic acid, 3-5% of glycerol, 0.01-0.1% of sodium hyaluronate, 0.5-1% of phytosterol, 0.5-1% of cetearyl alcohol, 0.5-2% of isononyl isononanoate, 1-6% of plant extract, 0.5-3% of hydroxyethyl cellulose, 0.2-0.3% of phenoxyethanol and the balance of water.

3. The skin care product of prebiotics and probiotics for treating acne according to any one of claims 1-2, wherein the prebiotics are oat β glucan, fructo-oligosaccharide, α -glucan oligosaccharide, inulin, galacto-oligosaccharide and trehalose, and the weight ratio is 2 (1-10).

4. The prebiotic and probiotic skin care product for the treatment of acne according to claims 1-2 wherein the probiotic is bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae, lactobacillus reuteri in a mass ratio of 3: (1-10): (1-10): (1-10): (1-10): (1-10).

5. The prebiotic and probiotic skin care product for the treatment of acne according to any of claims 1 to 2, characterized in that said plant extract is a horse chestnut extract, a wild soybean extract, a red sage root extract, a centella asiatica extract, a phellodendron amurense extract, in a weight ratio of 1: (1-10): (1-10): (1-10): (1-10).

6. The prebiotic and probiotic skin care product for the treatment of acne as defined in any of claims 1 to 2, comprising the following components in mass percent: 2% polyoxyethylene 100 stearate, 2.5% caprylic capric triglyceride, 3% stearic acid, 0.5% phytosterol, 1% cetearyl alcohol, 0.5% isononyl isononanoate, 5% prebiotics, 3% probiotics, 3% glycerol, 0.06% sodium hyaluronate, 1.5% hydroxyethyl cellulose, 5% plant extract, 0.25% phenoxyethanol, 72.69% water;

the probiotics comprise bifidobacterium longum, lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus plantarum, saccharomyces cerevisiae and lactobacillus reuteri, and the mass ratio is 3: 2: 4: 2: 2: 1;

7. the method for preparing prebiotic and probiotic skin care product for the treatment of acne as defined in claim 1, comprising the steps of: