CN113925923B - Anti-acne and whitening compound probiotic composition and preparation method thereof - Google Patents
Anti-acne and whitening compound probiotic composition and preparation method thereof Download PDFInfo
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
Abstract
The invention discloses an anti-acne and whitening composite probiotic composition and a preparation method thereof, wherein the anti-acne and whitening composite probiotic composition is prepared from the following components in parts by mass (20 to 60): (40 to 100): (30 to 70) consisting of probiotics, prebiotics and active compound; the probiotics is one or any combination of lactobacillus plantarum, lactobacillus paracasei, bifidobacterium animalis subsp lactis, bifidobacterium longum, lactobacillus rhamnosus and lactobacillus reuteri. The anti-acne and whitening composite probiotic composition provided by the invention is an oral product specially designed for complex pathogenesis of dark yellow skin and acne, can adjust the balance of probiotics in a human body, effectively treats the acne and the dark yellow skin from inside to outside and from the root through multiple ways, has a remarkable effect, is stable in curative effect, is not easy to relapse, and has no toxic or side effect and skin irritation.
Description
Technical Field
The invention relates to the technical field of probiotics, in particular to an anti-acne and whitening compound probiotic composition and a preparation method thereof.
Background
The skin surface has a large and complex microbial ecosystem to regulate skin health, and the human microbial ecosystem affects the system from the root. With the progress of scientific research, more and more researches show that the skin condition has close relation with the micro-ecology of human body, and we should not regard the various conditions of the skin as "problems" but send out signals from the body, which indicate the root-borne problems of our body. When the probiotic bacteria in the body are out of balance, a variety of skin problems may result, and some signs of aging may also be accelerated. Common skin problems such as pox are associated with imbalances in the commensal strains caused by diet or stress; the abnormal ageing of the skin is linked to the harmful oxidative metabolism of the intestinal microorganisms and is associated with the accumulation of toxins by the intestinal pathogens; dullness of skin color is an indication of skin aging and is associated with excessive accumulation and migration of pigments in the human intestinal tract.
For a long time, many studies have been devoted to the directions of skin dark yellow, acne removal, and the like. For example, the formation mechanism of dark yellow skin color is studied in the thesis "the formation mechanism of dark yellow skin color is symmetrical in skin aging species, gu Jianmei, etc., and in chinese cosmetic medicine 2011, 12 th volume 20, 12 th volume". The study on the pathogenesis of acne is carried out in the thesis of mechanism symptoms of acne and efficacy types of acne-removing skin care products, he Lingling and the like, daily chemical industry, volume 50, stage 5). There are also many products on the market for removing acne and acne on dark yellow skin, but the existing whitening acne-removing products have the following defects: most of the probiotics products with whitening and anti-acne effects on the market at present are external products, the strains are single, the effect is not obvious, after people use the external products, the probiotics in intestinal tracts of human bodies are still in an unbalanced state, the skin whitening effect is not good, and acne is not grown and does not relapse regularly.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide an anti-acne and whitening compound probiotic composition.
The invention also aims to provide a preparation method of the anti-acne and whitening compound probiotic composition.
One of the purposes of the invention is realized by adopting the following technical scheme:
an anti-acne and whitening compound probiotic composition comprises the following components in percentage by mass (20 to 60): (40 to 100): (30 to 70), wherein the active compound comprises an active component, or comprises the active component and an auxiliary material; the probiotics are one or any combination of Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus paracasei (Lactobacillus paracasei), bifidobacterium animalis subsp (Bifidobacterium animalis subsp. Lactis), bifidobacterium longum (Bifidobacterium longum), lactobacillus rhamnosus (Lactobacillus rhamnosus) and Lactobacillus reuteri (Lactobacillus reuteri); the prebiotics are one or any combination of fructo-oligosaccharide, inulin, xylo-oligosaccharide, galacto-oligosaccharide, malto-oligosaccharide, isomalto-oligosaccharide, resistant dextrin, soybean oligosaccharide, stachyose, chitosan and raffinose; the active component is one or any combination of lemon extract, raspberry powder, cherry powder, aloe extract, emblic leafflower fruit extract and tremella polysaccharide.
Furthermore, the viable count of the probiotics is more than or equal to 1 multiplied by 10 10 CFU/g。
Further, the Lactobacillus plantarum is Lactobacillus plantarum fed 8 (Lactobacillus plantarum fed 8); the Lactobacillus paracasei is Lactobacillus paracasei K9 (Lactobacillus paracasei K9); the Bifidobacterium animalis subsp lactis Y6 is Bifidobacterium animalis subsp lactis Y6; the Bifidobacterium longum is Bifidobacterium longum W68 (Bifidobacterium longum W68); the Lactobacillus rhamnosus is Lactobacillus rhamnosus YGG; the Lactobacillus reuteri is Lactobacillus reuteri K07 (Lactobacillus reuteri K07).
Further, the probiotic bacteria comprise at least Lactobacillus plantarum (Lactobacillus plantarum) and Bifidobacterium longum (Bifidobacterium longum).
Further, the probiotic bacteria include at least Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus paracasei (Lactobacillus paracasei), bifidobacterium animalis subsp.
Further, the mass ratio of Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus paracasei (Lactobacillus paracasei), bifidobacterium animalis subsp. (1: -2): (1~4): (1~3).
Further, the prebiotics at least comprise the following components in mass ratio of (15 to 30): 10: (1~5): (3~5): (2~4) fructooligosaccharides, galactooligosaccharides, resistant dextrins, soy oligosaccharides, and stachyose.
Further, the active components at least comprise the following components in a mass ratio of (1~8): (5 to 10): (4 to 10) emblic leafflower fruit extract, tremella polysaccharide and raspberry powder.
Further, the auxiliary materials are one or any combination of maltodextrin, lactose, vitamin C, vitamin E, xylitol, erythritol, sorbitol, citric acid, malic acid, lactic acid and the like.
Further, the probiotic consists of Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus paracasei (Lactobacillus paracasei), bifidobacterium animalis subsp. The prebiotics consist of resistant dextrin, fructo-oligosaccharide, galacto-oligosaccharide, soybean oligosaccharide and stachyose with the mass ratio of 15; the active compound comprises erythritol, xylitol, emblic leafflower fruit extract, citric acid, tremella polysaccharide, raspberry powder, vitamin C and vitamin E according to a mass ratio of 30.
The second purpose of the invention is realized by adopting the following technical scheme:
the preparation method of the anti-acne and whitening compound probiotic composition comprises the following steps: weighing probiotics, prebiotics and active compound according to the formula amount, and mixing uniformly.
Compared with the prior art, the invention has the beneficial effects that:
(1) The anti-acne and whitening composite probiotic composition provided by the invention is different from a conventional external product, is an internal product specially designed for complex pathogenesis of acne skin and acne, can adjust the balance of probiotics in a human body, effectively treats the acne and the acne of the skin from inside to outside and from the root through multiple ways, and has the advantages of obvious effect, stable curative effect, relapse prevention, no toxic or side effect and no skin irritation.
(2) The preparation method of the anti-acne and whitening composite probiotic composition provided by the invention is simple in process, simple and convenient to operate and easy to realize industrial production.
Biological material preservation information:
the strain provided by the invention, lactobacillus plantarum FEED8, has a preservation number of CGMCC No.15029, and is classified and named as: lactobacillus plantarum, which has been deposited in the general microorganism center of China Committee for culture Collection of microorganisms (address: no. 3, west Lu No.1 of Beijing, kyoho, the institute of microbiology, national academy of sciences, postal code: 100101) in 2017, 12.07.12.7.M.;
the lactobacillus paracasei K9 has a preservation number of CGMCC No.15025 and is classified and named as: lactobacillus paracasei, which has been deposited in the China general microbiological culture Collection center (address: no. 3, institute of microbiology, china academy of sciences, and postal code: 100101) of China Committee for culture Collection of microorganisms in 12.01.12.2017.A., lactobacillus paracasei, which has been deposited in CGMCC (China general microbiological culture Collection center) (address: no. 3, ministry of sciences, china, ministry of Japan);
bifidobacterium animalis subspecies lactis Y6, the preservation number is CGMCC NO.15026, and the classification and the name are as follows: bifidobacterium animalis subsp. Lactis, which has been deposited in the China general microbiological culture Collection center (address: ministry No. 3, institute of microbiology of China academy of sciences, ministry of sciences, and postal code: 100101) at 12.7.2017.12.7.2017.D. in the China Committee for culture Collection of microorganisms, the abbreviation of the unit of deposition is CGMCC;
bifidobacterium longum W68 with preservation code of CGMCC No.15028, is classified and named as: bifidobacterium longum has been deposited in the China general microbiological culture Collection center (address: no. 3 of West Lu No.1 of Beijing, kyoho area, microbial research institute of Chinese academy of sciences, postal code: 100101) in 07/12.2017, and the abbreviation of the unit of deposition is CGMCC;
the Lactobacillus rhamnosus YGG, the preservation number is CGMCC No.15027, and the classification and the name are as follows: lactobacillus rhamnosus, which has been deposited in the China general microbiological culture Collection center (address: no. 3, institute of microbiology, china academy of sciences, microbiol., postal code: 100101) of China Committee for culture Collection of microorganisms at 12.07.12.2017.8.;
lactobacillus reuteri K07, with preservation number CGMCC No.15703, is classified and named as: lactobacillus reuteri, which has been deposited in 28.04.2018 in China general microbiological culture Collection center (address: west Lu No.1, ministry of microbiology, national academy of sciences, ministry of microbiology, ministry of Japan, beijing, and Chacesterling, no. 3, china), the abbreviation of the unit for preservation is CGMCC.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, various embodiments or technical features described below may be arbitrarily combined to form a new embodiment. The raw materials, equipments and the like used in the following examples are commercially available unless otherwise specified.
The invention designs an anti-acne and whitening compound probiotic composition specially aiming at the complex pathogenesis of dark yellow skin and acneThe acne and the dark yellow skin can be effectively treated from inside to outside and from the root through multiple ways, and the acne and the dark yellow skin are obvious in effect, stable in curative effect, difficult to relapse, and free of toxic and side effects and skin irritation. 9 formulas are formed according to the components and the dosage of the following table 1 for testing, the probiotics used in each formula are the probiotic freeze-dried powder, and the viable count of the probiotic freeze-dried powder in the formula is more than or equal to 1 multiplied by 10 10 CFU/g。
Table 1 verification of the formulation
And (3) weighing the components according to the formula amount for the 9 formulas respectively, and then uniformly mixing to obtain the product.
Evaluation of Effect
1. Human body test
A subject: 110 healthy young people (55 men; 55 women) aged 15-45 years, with skin characterized by oily skin, mild and moderate whelk skin, all asians and studied and worked in Guangdong province.
The test method comprises the following steps: the test population was divided into 11 groups of 10 persons each, with a test period of 3 months. Of these 10 groups used samples of formula 1~9 and a commercial product, respectively, while the other 1 group did not use any acne treatment product (blank group). The testers took 1 bag of the composition each half an hour after lunch and dinner. The testers need to self-evaluate the use feelings of skin whitening, acne eliminating effect, repeated occurrence of acne, anti-inflammatory and soothing effect and the like. The evaluation score is 0 to 10 points (see table 2 in detail), and the test results are shown in table 3.
The commercial product is ROHTO ROHTOMELANO-CC essence which is a Japanese popular product and contains unnatural antioxidant and anti-inflammatory components such as vitamin C, vitamin E, dipotassium glycyrrhizinate, isopropyl methyl phenol and the like, and whitening and acne removal are realized by an external application method.
TABLE 2 evaluation criteria for volunteer evaluation scores and feeling
Table 3 verification of the efficacy of the skin-whitening anti-acne probiotic composition for the population
The record in the table 3 shows that the whitening acne-removing effects of the formulas 1 to 9 are superior to those of products sold on the market, after the skin-whitening acne-removing powder is taken for 3 months, a subject has better experience in the aspects of whitening, removing acne, reducing acne recurrence, resisting inflammation and relieving, and the total effective rate of whitening and removing acne reaches over 90 percent. The score of reducing the acne recurrence of formula 7 is up to 9.5, which shows that the acne of the subject is not recurrent basically after being cured and the acne removing effect is not rebounded.
The anti-acne and whitening compound probiotic composition provided by the invention is prepared by combining compound multi-probiotic bacteria, reasonably planning the proportion of each probiotic bacteria, and adding prebiotics, other active components and auxiliary materials, so that the whitening and anti-acne effects of the composition can be enhanced. By orally taking the composite probiotic composition with the effects of whitening and resisting acne, the whitening and acne-removing active probiotics are efficiently colonized in the intestinal tract, the intestinal microenvironment is improved, the growth and reproduction of harmful bacteria in the intestinal tract are inhibited, the intestinal endotoxin level is reduced, the inflammation and stress reaction are effectively reduced, the sensitive muscle is repaired, and acne removal is realized from the root. In addition, the composition has excellent antioxidant capacity, can delay organism aging, reduce pigment transfer in vivo, solve the problems of color spots, dark yellow skin and the like caused by peroxidation, and promote the white and bright skin. Probiotics, prebiotics, active complexes were further analyzed below:
firstly, the anti-acne and whitening composite probiotic composition provided by the invention, lactobacillus plantarum FEED8, is a core strain of the composite probiotic freeze-dried powder with whitening and anti-acne effects, and has more remarkable effect with the increase of the dosage and proportion (obtained by formulas 1, 2, 4 and 8). The active lactobacillus plantarum FEED8 is colonized in intestinal tracts, can continuously generate active oxygen (ROS) in a living body, comprises DPPH free radicals, hydroxyl free radicals (.OH), superoxide anions (O2. -), and the like, and can consolidate an antioxidant defense system and a repair system of the active lactobacillus plantarum FEED8 to maintain the ROS in the body in a balanced state. Due to its excellent antioxidant ability, the problems of color spots, wrinkles and aging caused by peroxidation can be effectively solved.
The bifidobacterium animalis subspecies lactis Y6 and the lactobacillus paracasei K9 have the functions of coordination and complementation, and the combination of the two can be used as the dominant strain combination for whitening and resisting the acne. Wherein, the bifidobacterium animalis subsp lactis Y6 can be efficiently colonized in the intestinal tract, improve the microenvironment of the intestinal tract, reduce inflammation and stress reaction and repair sensitive muscles. The lactobacillus paracasei K9 can reduce the bacterial shift in the intestinal tract, improve the dysbacteriosis condition of the intestinal tract, repair the intestinal tract mucosa, recover the barrier function of the intestinal tract, relieve the anaphylactic reaction caused by BLG, has better relieving effect than other strains belonging to the same genus, simultaneously increases the TGF-beta level in the serum, corrects the unbalance of lymphocyte Th1/Th2 in an allergic organism and relieves the skin anaphylactic symptom. The two ingredients act synergistically to strengthen the skin immune barrier and lighten the skin.
The bifidobacterium longum W68 and the lactobacillus rhamnosus YGG can be used as auxiliary strains of the composite probiotic freeze-dried powder with the effects of whitening and resisting acne. And the bifidobacterium longum W68 has stronger auxiliary effect, so the bifidobacterium longum is selected as a dominant auxiliary strain. The bifidobacterium longum W68 is acid-resistant, cholate-resistant and oxygen-resistant, can inhibit pathogenic bacteria in intestinal colonization, can secrete cytokines to regulate intestinal immunity, can inhibit intestinal harmful bacteria, delays organism aging and promotes skin youth. The auxiliary effect of the Lactobacillus rhamnosus YGG is weaker, and the Lactobacillus rhamnosus YGG can not be selected as one of the strains of the probiotic freeze-dried powder.
The lactobacillus reuteri K07 has certain inhibition effect on the efficacy of the composite probiotic freeze-dried powder with the whitening and anti-acne efficacy, and is not suitable to be used as one of the strains of the probiotic freeze-dried powder.
The optimal composition of the composite probiotic freeze-dried powder with the whitening and anti-acne effects provided by the invention is' lactobacillus plantarum FEED8: lactobacillus paracasei K9: bifidobacterium animalis subsp lactis Y6: bifidobacterium longum W68= 16.
And secondly, the fructo-oligosaccharide is the core prebiotics of the anti-acne and whitening composite probiotic composition, and the ratio of the fructo-oligosaccharide to the prebiotics is 40-45 percent in the best way. Fructooligosaccharides, as a prebiotic, have the effect of binding metal ions, which in the gastrointestinal tract form FOS-mineral complexes, which are released after reaching the large intestine. Fructo-oligosaccharide is fermented by intestinal probiotics to produce short-chain fatty acids such as acetic acid, propionic acid and butyric acid, lactic acid and the like, reduce the pH of the intestinal environment, inhibit the growth and reproduction of harmful bacteria and pathogenic bacteria, reduce the generation and accumulation of enterotoxin and putrefactive substances, keep the skin healthy and reduce the risk of pox and pox recurrence. The reduction of the pH value of the intestinal environment is also beneficial to the dissolution of mineral salts such as calcium, magnesium and the like, thereby promoting the absorption of the mineral elements such as calcium, magnesium and the like by the body. The fructo-oligosaccharide can proliferate probiotics and enhance the probiotic effect of the probiotics. The fructo-oligosaccharide can also promote the synthesis of vitamins B1, B2, B3, B6 and B12 and folic acid, thereby improving the metabolism level of human body and keeping the skin young.
The optimal composition of the prebiotics of the composite probiotic composition with the effects of whitening and resisting acne provided by the invention is' resistant dextrin: fructo-oligosaccharide: galacto-oligosaccharide: soybean oligosaccharide: stachyose = 15.
Finally, the emblic extract, the tremella polysaccharide and the raspberry powder are core effect auxiliary materials of the compound probiotic composition with the whitening and anti-acne effects, and the proportion of the emblic extract, the tremella polysaccharide and the raspberry powder is 4:4:5 has obvious strengthening effect on the whitening and acne removing efficacy of the composite probiotic composition.
Wherein, the emblic leafflower fruit contains 12 vitamins, 16 microelements, 18 amino acids, organic acid, protein, sugar and the like. The content of vitamin C in the emblic leafflower fruits is high and stable, and the average content of the vitamin C is 100 times that of citrus, 160 times that of apples and 5 times that of kiwi fruits. In addition, the emblic extract can inhibit the interaction between the DNA of pathogenic microorganisms and transcription factors (NF-KB/DNA), so that the transcription of the pathogenic microorganisms is inhibited, and the emblic extract contains a large amount of oleanolic acid, has obvious inhibition effect on staphylococcus, typhoid bacillus, escherichia coli and dysentery bacillus, and is beneficial to reducing the endotoxin level of intestinal tracts and keeping the skin healthy.
Tremella, also known as Tremella fuciformis and Tremella fuciformis, belongs to Tremellaceae of Basidiomycetes, is a traditional medicinal edible fungus in China, and is a tonic for treating asthenia and aging. The tremella contains various components such as polysaccharide, flavone and amino acid, and has the effects of whitening and refreshing skin, wherein the tremella polysaccharide is an important active substance of the tremella polysaccharide. Research shows that the tremella polysaccharide plays a role in resisting aging by regulating transcription and expression of a negative regulation factor P21 of a cell cycle, resisting oxidation and enhancing an immune function, and can effectively keep young skin, so the tremella polysaccharide can be used as a potential therapeutic agent for treating skin diseases and aging related to oxidative stress, and can prevent and treat pox recurrence.
Raspberry, also called raspberry, sanyue bubble, april bubble, mountain throw, thorn gourd, raspberry, steamed bun spinach, high-foot spinach, dragon boat bubble, milk bubble, and spine, is perennial deciduous shrub of rubus of Rosaceae, and is called raspberry because its fruit shape, color, and taste are similar to strawberry but growing on the tree, and its fruit shape is more than 750. The vitamin E content is at the head of various fruits, and is rich in superoxide dismutase and vitamin C. The natural superoxide dismutase and the vitamin E are excellent human body scavenger, can eliminate a large amount of harmful metabolites generated by a human body, improve the immunity of the human body, fundamentally improve the internal environment of the human body, and achieve the purposes of maintaining beauty, keeping young and prolonging life.
In summary, the formula 7 is the best embodiment of the present invention, and the best combination of the composite probiotic composition with skin whitening and anti-acne effects provided by the present invention is "composite probiotic freeze-dried powder: prebiotics: active complex =3:4:3 ', wherein the optimal combination of the composite probiotic freeze-dried powder is' Lactobacillus plantarum FEED8: lactobacillus paracasei K9: bifidobacterium animalis subsp lactis Y6: bifidobacterium longum W68=16, 1", the best prebiotic combination being" resistant dextrin: fructo-oligosaccharide: galacto-oligosaccharide: soybean oligosaccharide: stachyose =15, 1: white fungus polysaccharide: raspberry powder =4:4:5".
2. Toxicology test of animals
A subject: 40 adult healthy guinea pigs of the Netherlands species, animal grade I, body weight 200-300g, and sex half. Grouping by a random number arrangement table method during experiments, and feeding in cages.
The test method comprises the following steps: each batch was divided into two groups, placebo (vehicle), sample (formula 7), 10 guinea pigs each, by a random number permutation table method. Using a random double blind control experiment, both compositions were packaged in a unified package, with only the outer package being labeled with the A, B script. The hair on two sides of spinal column of animal is shaved with razor 24h before administration, and the hair removal range on each side is 4 × 4cm 2 . The right depilated skin of each guinea pig was set as the intact skin test area and the left depilated skin was set as the broken skin test area (stratum corneum was lacerated with a sterile syringe needle without damaging the dermis, allowing abrasion with a similar toenail scratch but without bleeding). Observation indexes are as follows: after administration, the animals were observed daily for changes in their general condition including changes in the skin, hair, eyes and mucous membranes, changes in respiration, limb movements, etc. And if dead animals exist, necropsy and pathological examination are required, and histopathological examination is carried out on the skin of the application part of all the animals after the experiment is finished.
Acute toxicity test: animals were shaved 24h after which A, B were fed vehicle, formula 7 composition, 0.1g each time, 2 times daily for 1 week continuously.
Long-term toxicity test: animals were shaved 24h after which A, B were fed vehicle, formula 7 composition, 0.1g each time, 2 times daily for 4 weeks. And observed for 2 weeks after withdrawal to determine whether the test substance has a toxic response and the extent of reversibility of the toxic response.
The experimental results are as follows:
acute toxicity test: within 7d after administration of the blank control group (vehicle) and the sample group (formula 7), the animals had intact skin and damaged skin without erythema and edema, and the behavioral activities, diet, coat luster, body weight, mental state, feces, skin color, respiration, secretion, eyes and mucous membranes of the guinea pigs were normal, so all animals survived healthily. It is fully shown that formula 7 has no toxic side effects and no skin irritation.
Long-term toxicity test: all guinea pigs were normal for behavioral activity, diet, body weight, mental status, feces, respiration and secretions, eyes and mucous membranes, and all animals were healthy for survival 4 weeks after administration of the placebo group (vehicle), sample group (formula 7). It is fully shown that formula 7 has no toxic side effects and no skin irritation.
In conclusion, the invention is prepared by taking the pathogenesis and clinical manifestation of skin aging and acne as the research basis, the aim of whitening and removing acne as the aim, the scientific compounded active concatenated probiotic freeze-dried powder as a main effect additive and the auxiliary preparation of natural medicinal and edible plant components. The product is completely different from the commercially available whitening and acne-removing products, because the skin problem is revealed in the form, the conventional treatment mode is mostly direct treatment at the affected part, so the commercially available products are mostly externally applied medicines, and because the treatment is not carried out from the root, the problems of unobvious curative effect, acne recurrence and the like often exist.
The invention creatively performs whitening and acne removal from the root from inside to outside, and is the first innovative product with obvious effect on the market. The composite probiotic composition with the effects of whitening and resisting acne provided by the invention has the effects of resisting inflammation and relieving, reducing acne recurrence, resisting aging, resisting oxidation, reducing pigment transfer in vivo and the like, realizes effective treatment on skin aging dark yellow and acne through multiple ways, has no toxic or side effect and skin irritation, contains the multi-probiotic composition with the whitening and acne removing activities in the formula, is specially designed aiming at the complex pathogenesis of skin aging dark yellow and acne, regulates the microecology of a human body from inside to outside, realizes whitening and skin-reviving and acne removal from the root, and is safe, effective and free of recurrence. Meanwhile, the composition is convenient to eat, so that the composition can be popularized and eaten in people.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (8)
1. An anti-acne and whitening compound probiotic composition is characterized by comprising the following components in parts by mass (20 to 60): (40 to 100): (30 to 70), wherein the active compound comprises an active component, or comprises the active component and an auxiliary material;
the probiotic is Lactobacillus plantarum (Lactobacillus plantarum); or the probiotics are Lactobacillus plantarum (Lactobacillus plantarum), and composite probiotics formed by combining with one or any of the following probiotics: lactobacillus paracasei (Lactobacillus paracasei), bifidobacterium animalis subsp (lactis), bifidobacterium longum (Bifidobacterium longum), and Lactobacillus rhamnosus (Lactobacillus rhamnosus);
the prebiotics comprise the following components in percentage by mass (15 to 30): 10: (1~5): (3~5): (2~4) fructooligosaccharides, galactooligosaccharides, resistant dextrins, soy oligosaccharides, and stachyose;
the active components comprise (1~8): (5 to 10): (4 to 10) emblic leafflower fruit extract, tremella polysaccharide and raspberry powder.
2. The anti-acne and whitening composite probiotic composition according to claim 1, characterized in that the Lactobacillus plantarum is Lactobacillus plantarum ed8 (Lactobacillus plantarum ed 8) having accession number CGMCC No. 15029; the Lactobacillus paracasei is Lactobacillus paracasei K9 (Lactobacillus paracasei K9) with the preservation number of CGMCC No. 15025; the Bifidobacterium animalis subsp lactis Y6 with the preservation number of CGMCC No. 15026; the Bifidobacterium longum is Bifidobacterium longum W68 (Bifidobacterium longum W68) with the preservation number of CGMCC No. 15028; the Lactobacillus rhamnosus is Lactobacillus rhamnosus YGG (Lactobacillus rhamnous YGG) with the preservation number of CGMCC No. 15027.
3. The anti-acne and whitening composite probiotic composition according to claim 1, characterized in that the probiotics comprise at least Lactobacillus plantarum (Lactobacillus plantarum) and Bifidobacterium longum (Bifidobacterium longum).
4. The anti-acne and whitening composite probiotic composition according to claim 1, characterized in that the probiotics comprise at least Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus paracasei (Lactobacillus paracasei), bifidobacterium animalis subsp.
5. The anti-acne and whitening composite probiotic composition according to claim 4, characterized in that the mass ratio of Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus paracasei (Lactobacillus paracasei), bifidobacterium animalis subsp. (1~2): (1~4): (1~3).
6. The anti-acne and whitening composite probiotic composition according to claim 1, characterized in that the auxiliary material is one or any combination of maltodextrin, lactose, vitamin C, vitamin E, xylitol, erythritol, sorbitol, citric acid, malic acid and lactic acid.
7. The anti-acne and whitening composite probiotic composition according to claim 1, characterized in that the probiotics consist of Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus paracasei (Lactobacillus paracasei), bifidobacterium animalis subsp. The prebiotics consist of resistant dextrin, fructo-oligosaccharide, galacto-oligosaccharide, soybean oligosaccharide and stachyose with the mass ratio of 15; the active compound comprises erythritol, xylitol, emblic leafflower fruit extract, citric acid, tremella polysaccharide, raspberry powder, vitamin C and vitamin E according to a mass ratio of 30.
8. A method for preparing the anti-acne and whitening composite probiotic composition according to any one of claims 1 to 7, characterized by comprising the following steps:
weighing: weighing probiotics, prebiotics and active compound according to the formula amount; if the formula also comprises auxiliary materials, weighing the auxiliary materials according to the formula amount;
mixing: and (3) uniformly mixing all the components weighed in the weighing step to obtain the composition.
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| CN112980892A (en) * | 2021-03-02 | 2021-06-18 | 山东艾益典生物技术有限公司 | Composite probiotic fermented product with skin care effect and preparation and application thereof |
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| CN112980892A (en) * | 2021-03-02 | 2021-06-18 | 山东艾益典生物技术有限公司 | Composite probiotic fermented product with skin care effect and preparation and application thereof |
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