CN113197814A - 一种玻尿酸微针贴片制备方法 - Google Patents
一种玻尿酸微针贴片制备方法 Download PDFInfo
- Publication number
- CN113197814A CN113197814A CN202110402936.7A CN202110402936A CN113197814A CN 113197814 A CN113197814 A CN 113197814A CN 202110402936 A CN202110402936 A CN 202110402936A CN 113197814 A CN113197814 A CN 113197814A
- Authority
- CN
- China
- Prior art keywords
- microneedle
- solution
- hyaluronic acid
- extract
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims abstract description 46
- 229920002674 hyaluronan Polymers 0.000 title claims abstract description 46
- 229960003160 hyaluronic acid Drugs 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 239000000243 solution Substances 0.000 claims abstract description 44
- 239000000284 extract Substances 0.000 claims abstract description 32
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
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- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 claims abstract description 13
- 102000008186 Collagen Human genes 0.000 claims abstract description 13
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- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 claims abstract description 13
- 229940106189 ceramide Drugs 0.000 claims abstract description 13
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229920001436 collagen Polymers 0.000 claims abstract description 13
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 claims abstract description 13
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- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims description 8
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- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
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- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
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Classifications
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Landscapes
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- Cosmetics (AREA)
Abstract
本发明涉及一种玻尿酸微针贴片制备方法，包括以下原材料：微针溶液：透明质酸10‑15％、活性提取物5‑10％、神经酰胺3‑6％、水解胶原蛋白4‑8％、积雪草6‑12％、防腐剂0.5‑2％和去离子水60‑72％，基层原液：基质材料聚合物和去离子水，固液比为1:3，所述制备方法包括以下步骤：(1)微针溶液的制备：称取去离子水60‑72％，缓慢加入称量好的透明质酸10‑15％，待透明质酸完全溶胀后，加入活性提取物5‑10％、神经酰胺3‑6％、水解胶原蛋白4‑8％、积雪草6‑12％、防腐剂0.5‑2％，加热并提高搅拌速度直至溶液沸腾；(2)基层原液的制备：称取基质材料按照固液比为1:3加入去离子水，搅拌至溶解。本发明采用微针将天然活性成分送达皮肤内部，具有保湿、美白祛斑、延缓衰老、抗过敏、抗菌抗炎等功效。
Description
技术领域
本发明涉及护肤品技术领域，具体是指一种玻尿酸微针贴片制备方法。
背景技术
玻尿酸，又名透明质酸，是由葡糖醛酸和N-乙酰氨基葡糖为重复单位组成直链高分子多糖，是一种广泛存在于脊椎动物组织细胞外基质的生物大分子物质，在医药领域透明质酸又被称作玻璃酸，目前透明质酸的商品形式均为其钠盐。当玻尿酸涂于皮肤表面时，会形成一层具有黏弹性的透明水化膜，起到优良的保水功效，而且具有良好的透气性，无油腻感，令皮肤感觉自然，光滑透软。玻尿酸以其特有的保湿功效和理化兴性质，已成为国家上公认的优良天然保湿因子。
微针技术是一种新型的无痛微创给药系统，结合了透皮贴剂和注射剂型的优点，能够穿透皮肤角质层屏障，为大分子药物如蛋白质、疫苗等药物提供了新型的给药途径，克服了大分子药物的口服、透皮吸收问题。微针技术已逐渐成为医药领域、美容领域、微生物领域、精密制造领域的研究热点。
目前市面上的玻尿酸护肤产品种类繁多，但是大多数的产品作用仅停留在皮肤表层，护肤效果不够显著。金属微针皮肤穿透性高，但是对皮肤的创伤较大；硅微针有良好的传感性能和机电性能，但是硅微针容易断裂。因此，很有必要研发一种既能促进皮肤对玻尿酸的有效吸收，性能又十分良好的玻尿酸微针贴片。
发明内容
本发明的目的是提供一种玻尿酸微针贴片制备方法，以玻尿酸、神经酰胺、水解胶原蛋白等为主要成分，该产品具有保湿、抗皱、美白、抗炎等作用。
为解决上述技术问题，本发明提供的技术方案为：
一种玻尿酸微针贴片制备方法，包括以下原材料：微针溶液：透明质酸10-15％、活性提取物5-10％、神经酰胺3-6％、水解胶原蛋白4-8％、积雪草6-12％、防腐剂0.5-2％和去离子水60-72％，基层原液：基质材料聚合物和去离子水，固液比为1:3，所述制备方法包括以下步骤：
(1)微针溶液的制备：称取去离子水60-72％，在低速搅拌的条件下，缓慢加入称量好的透明质酸10-15％，待透明质酸完全溶胀后，加入活性提取物5-10％、神经酰胺3-6％、水解胶原蛋白4-8％、积雪草6-12％、防腐剂0.5-2％，加热并提高搅拌速度直至溶液沸腾，超声去除溶液中的气泡；
(2)基层原液的制备：称取基质材料按照固液比为1:3加入去离子水，搅拌至溶解，超声去除溶液中的气泡；
(3)模具的填充：取适量的微针溶液，倾倒于模具表面，均匀涂布后，使微针溶液进入并填满模具针状凹槽，将模具水平放置于真空干燥器内，打开真空泵，真空度为0.06-0.09MPa，连续抽真空1-1.5h，再加入基层原液，相同条件真空干燥；
(4)微针贴片的干燥：模具填充后，-80℃预冻1-2h，转入真空冷冻干燥机冻干24-36h；
(5)脱模：微针干燥至恒重后，缓慢剥离模具，即可得到微针贴片。
进一步的，所述防腐剂包括聚赖氨酸、苯甲酸、水杨酸或苯甲醇中的至少一种。
进一步的，所述活性提取物包括灵芝提取物、银耳提取物、芦荟提取物、红花提取物、丹参提取物、紫草根提取物、红景天提取物或乳香提取物中的至少一种。
进一步的，所述基质材料聚合物包括聚乳酸、聚乙醇酸、聚乳酸-乙醇酸、羟甲基纤维素、聚乙烯吡咯烷酮、硫酸软骨素、海藻糖、麦芽糖、半乳糖或蔗糖中的至少一种。
进一步的，所述模具使用聚四氟乙烯作为模具材料，模具凹槽为圆锥状，深度为100-500μm，圆锥底部直径为200-500μm。
采用以上制备方法，本发明具有如下优点：
微针属于中胚层疗法，微针作用于皮肤，刺入表皮内或表皮层下，形成无数微小通道，彻底破坏表皮牢固的屏障，使有效护肤成分经通道渗透，在皮下部位蓄积，足量直接作用，增强保湿、抗皱、抗氧化等护肤效果；玻尿酸具有高度的粘弹性、可塑性、渗透性以及独特的流变性能，是一种非常理想、安全可靠的生物材料和药物载体；本发明采用真空冷冻干燥机进行冻干，有效防止升温对微针内的活性成分的破坏；采用玻尿酸制备可溶性微针，可在皮肤内完全溶解，同时将内部包封的生物活性物质释放到人体皮肤内发挥作用，避免了金属微针对人体皮肤来的伤害和硅微针断裂的发生；添加多种天然活性提取物成分，减少了化学成分的使用，产品更加安全，赋予产品美白祛斑、延缓衰老、抗氧化、抗过敏、抗菌抗炎等功效。
具体实施方式
下面结合实施例对本发明做进一步的详细说明。
实施例一
一种玻尿酸微针贴片制备方法，包括以下原材料：微针溶液：透明质酸15％、活性提取物6％、神经酰胺3％、水解胶原蛋白4％、积雪草7％、聚赖氨酸1％和去离子水64％，基层原液：基质材料聚合物和去离子水，固液比为1:3，所述制备方法包括以下步骤：
(1)微针溶液的制备：称取去离子水64％，在低速搅拌的条件下，缓慢加入称量好的透明质酸15％，待透明质酸完全溶胀后，加入活性提取物6％、神经酰胺3％、水解胶原蛋白4％、积雪草7％、聚赖氨酸1％，加热并提高搅拌速度直至溶液沸腾，超声去除溶液中的气泡；
(2)基层原液的制备：称取基质材料按照固液比为1:3加入去离子水，搅拌至溶解，超声去除溶液中的气泡；
(3)模具的填充：取适量的微针溶液，倾倒于模具表面，均匀涂布后，使微针溶液进入并填满模具针状凹槽，将模具水平放置于真空干燥器内，打开真空泵，真空度为0.06MPa，连续抽真空1.5h，再加入基层原液，相同条件真空干燥；
(4)微针贴片的干燥：模具填充后，-80℃预冻1h，转入真空冷冻干燥机冻干24h；
(5)脱模：微针干燥至恒重后，缓慢剥离模具，即可得到微针贴片。
所述活性提取物为灵芝提取物和紫草根提取物。
所述基质材料聚合物为羟甲基纤维素。
所述模具使用聚四氟乙烯作为模具材料，模具凹槽为圆锥状，深度为100μm，圆锥底部直径为200μm。
实施例二
一种玻尿酸微针贴片制备方法，包括以下原材料：微针溶液：透明质酸13％、活性提取物5％、神经酰胺5％、水解胶原蛋白8％、积雪草8％、水杨酸1％和去离子水60％，基层原液：基质材料聚合物和去离子水，固液比为1:3，所述制备方法包括以下步骤：
(1)微针溶液的制备：称取去离子水60％，在低速搅拌的条件下，缓慢加入称量好的透明质酸13％，待透明质酸完全溶胀后，加入活性提取物5％、神经酰胺5％、水解胶原蛋白8％、积雪草8％、水杨酸1％，加热并提高搅拌速度直至溶液沸腾，超声去除溶液中的气泡；
(2)基层原液的制备：称取基质材料按照固液比为1:3加入去离子水，搅拌至溶解，超声去除溶液中的气泡；
(3)模具的填充：取适量的微针溶液，倾倒于模具表面，均匀涂布后，使微针溶液进入并填满模具针状凹槽，将模具水平放置于真空干燥器内，打开真空泵，真空度为0.09MPa，连续抽真空1h，再加入基层原液，相同条件真空干燥；
(4)微针贴片的干燥：模具填充后，-80℃预冻2h，转入真空冷冻干燥机冻干24h；
(5)脱模：微针干燥至恒重后，缓慢剥离模具，即可得到微针贴片。
所述活性提取物为银耳提取物、芦荟提取物、丹参提取物和紫草根提取物。
所述基质材料聚合物为硫酸软骨素。
所述模具使用聚四氟乙烯作为模具材料，模具凹槽为圆锥状，深度为500μm，圆锥底部直径为300μm。
实施例三
一种玻尿酸微针贴片制备方法，包括以下原材料：微针溶液：透明质酸14％、活性提取物6％、神经酰胺3％、水解胶原蛋白4％、积雪草6％、苯甲醇0.5％和去离子水66.5％，基层原液：基质材料聚合物和去离子水，固液比为1:3，所述制备方法包括以下步骤：
(1)微针溶液的制备：称取去离子水66.5％，在低速搅拌的条件下，缓慢加入称量好的透明质酸14％，待透明质酸完全溶胀后，加入活性提取物6％、神经酰胺3％、水解胶原蛋白4％、积雪草6％、苯甲醇0.5％，加热并提高搅拌速度直至溶液沸腾，超声去除溶液中的气泡；
(2)基层原液的制备：称取基质材料按照固液比为1:3加入去离子水，搅拌至溶解，超声去除溶液中的气泡；
(3)模具的填充：取适量的微针溶液，倾倒于模具表面，均匀涂布后，使微针溶液进入并填满模具针状凹槽，将模具水平放置于真空干燥器内，打开真空泵，真空度为0.08MPa，连续抽真空1.5h，再加入基层原液，相同条件真空干燥；
(4)微针贴片的干燥：模具填充后，-80℃预冻1h，转入真空冷冻干燥机冻干36h；
(5)脱模：微针干燥至恒重后，缓慢剥离模具，即可得到微针贴片。
所述活性提取物为芦荟提取物、红花提取物、红景天提取物和乳香提取物。
所述基质材料聚合物为聚乳酸和半乳糖。
所述模具使用聚四氟乙烯作为模具材料，模具凹槽为圆锥状，深度为300μm，圆锥底部直径为400μm。
以上所述，仅为本发明较佳的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，根据本发明的技术方案及其发明构思加以等同替换或改变，都应涵盖在本发明的保护范围之内。
Claims (5)
1.一种玻尿酸微针贴片制备方法，其特征在于，包括以下原材料：微针溶液：透明质酸10-15％、活性提取物5-10％、神经酰胺3-6％、水解胶原蛋白4-8％、积雪草6-12％、防腐剂0.5-2％和去离子水60-72％，基层原液：基质材料聚合物和去离子水，固液比为1:3，所述制备方法包括以下步骤：
(1)微针溶液的制备：称取去离子水60-72％，在低速搅拌的条件下，缓慢加入称量好的透明质酸10-15％，待透明质酸完全溶胀后，加入活性提取物5-10％、神经酰胺3-6％、水解胶原蛋白4-8％、积雪草6-12％、防腐剂0.5-2％，加热并提高搅拌速度直至溶液沸腾，超声去除溶液中的气泡；
(2)基层原液的制备：称取基质材料按照固液比为1:3加入去离子水，搅拌至溶解，超声去除溶液中的气泡；
(3)模具的填充：取适量的微针溶液，倾倒于模具表面，均匀涂布后，使微针溶液进入并填满模具针状凹槽，将模具水平放置于真空干燥器内，打开真空泵，真空度为0.06-0.09MPa，连续抽真空1-1.5h，再加入基层原液，相同条件真空干燥；
(4)微针贴片的干燥：模具填充后，-80℃预冻1-2h，转入真空冷冻干燥机冻干24-36h；
(5)脱模：微针干燥至恒重后，缓慢剥离模具，即可得到微针贴片。
2.根据权利要求1所述的一种玻尿酸微针贴片制备方法，其特征在于，所述防腐剂包括聚赖氨酸、苯甲酸、水杨酸或苯甲醇中的至少一种。
3.根据权利要求1所述的一种玻尿酸微针贴片制备方法，其特征在于，所述活性提取物包括灵芝提取物、银耳提取物、芦荟提取物、红花提取物、丹参提取物、紫草根提取物、红景天提取物或乳香提取物中的至少一种。
4.根据权利要求1所述的一种玻尿酸微针贴片制备方法，其特征在于，所述基质材料聚合物包括聚乳酸、聚乙醇酸、聚乳酸-乙醇酸、羟甲基纤维素、聚乙烯吡咯烷酮、硫酸软骨素、海藻糖、麦芽糖、半乳糖或蔗糖中的至少一种。
5.根据权利要求1所述的一种玻尿酸微针贴片制备方法，其特征在于，所述模具使用聚四氟乙烯作为模具材料，模具凹槽为圆锥状，深度为100-500μm，圆锥底部直径为200-500μm。
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| CN114010579A (zh) * | 2021-11-19 | 2022-02-08 | 烟台魔技纳米科技有限公司 | 一种可溶性抗炎修护微针及其制备方法 |
| CN114288544A (zh) * | 2021-12-14 | 2022-04-08 | 中化学科学技术研究有限公司 | 一种可溶性微针、微针贴片及二者的制备方法 |
| CN115737606A (zh) * | 2022-12-01 | 2023-03-07 | 浙江工业大学 | 一种基于冷冻干燥技术的多孔结构可溶性微针及其制备方法和应用 |
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