CN113181089B - Soothing and repairing composition and preparation method and application thereof - Google Patents
Soothing and repairing composition and preparation method and application thereof Download PDFInfo
- Publication number
- CN113181089B CN113181089B CN202110305740.6A CN202110305740A CN113181089B CN 113181089 B CN113181089 B CN 113181089B CN 202110305740 A CN202110305740 A CN 202110305740A CN 113181089 B CN113181089 B CN 113181089B
- Authority
- CN
- China
- Prior art keywords
- soothing
- skin
- parts
- preparation
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 71
- 238000002360 preparation method Methods 0.000 title claims abstract description 49
- 235000001855 Portulaca oleracea Nutrition 0.000 claims abstract description 46
- 244000234609 Portulaca oleracea Species 0.000 claims abstract description 44
- 230000000694 effects Effects 0.000 claims abstract description 32
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 23
- 235000006439 Lemna minor Nutrition 0.000 claims abstract description 22
- 238000000605 extraction Methods 0.000 claims abstract description 21
- 230000008591 skin barrier function Effects 0.000 claims abstract description 9
- 230000008439 repair process Effects 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 38
- 239000002994 raw material Substances 0.000 claims description 31
- 230000001954 sterilising effect Effects 0.000 claims description 29
- 239000004744 fabric Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 16
- 239000008367 deionised water Substances 0.000 claims description 15
- 229910021641 deionized water Inorganic materials 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 15
- 238000005303 weighing Methods 0.000 claims description 13
- 241000951473 Schizonepeta Species 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 8
- 230000003110 anti-inflammatory effect Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 241000219304 Portulacaceae Species 0.000 claims 2
- 230000003716 rejuvenation Effects 0.000 claims 2
- 230000007760 free radical scavenging Effects 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- 208000003251 Pruritus Diseases 0.000 abstract description 22
- 206010061218 Inflammation Diseases 0.000 abstract description 11
- 230000004054 inflammatory process Effects 0.000 abstract description 9
- 208000002193 Pain Diseases 0.000 abstract description 8
- 230000008859 change Effects 0.000 abstract description 7
- 230000005808 skin problem Effects 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 abstract description 5
- 239000002537 cosmetic Substances 0.000 abstract description 4
- 230000003993 interaction Effects 0.000 abstract description 3
- 238000011160 research Methods 0.000 abstract description 2
- 230000003064 anti-oxidating effect Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 61
- 210000003491 skin Anatomy 0.000 description 40
- 238000012360 testing method Methods 0.000 description 23
- 239000000243 solution Substances 0.000 description 21
- 150000003254 radicals Chemical class 0.000 description 19
- 239000000284 extract Substances 0.000 description 18
- ACCCMOQWYVYDOT-UHFFFAOYSA-N hexane-1,1-diol Chemical compound CCCCCC(O)O ACCCMOQWYVYDOT-UHFFFAOYSA-N 0.000 description 17
- UWJJYHHHVWZFEP-UHFFFAOYSA-N pentane-1,1-diol Chemical compound CCCCC(O)O UWJJYHHHVWZFEP-UHFFFAOYSA-N 0.000 description 17
- 206010015150 Erythema Diseases 0.000 description 14
- 230000003260 anti-sepsis Effects 0.000 description 13
- 230000007803 itching Effects 0.000 description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 12
- 238000004659 sterilization and disinfection Methods 0.000 description 12
- 239000002158 endotoxin Substances 0.000 description 10
- 229920006008 lipopolysaccharide Polymers 0.000 description 10
- 230000000638 stimulation Effects 0.000 description 10
- 230000008961 swelling Effects 0.000 description 10
- 102000003777 Interleukin-1 beta Human genes 0.000 description 8
- 108090000193 Interleukin-1 beta Proteins 0.000 description 8
- 206010040844 Skin exfoliation Diseases 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000035618 desquamation Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 230000002757 inflammatory effect Effects 0.000 description 7
- 230000002195 synergetic effect Effects 0.000 description 7
- YKPUWZUDDOIDPM-SOFGYWHQSA-N capsaicin Chemical compound COC1=CC(CNC(=O)CCCC\C=C\C(C)C)=CC=C1O YKPUWZUDDOIDPM-SOFGYWHQSA-N 0.000 description 6
- 239000006143 cell culture medium Substances 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 6
- -1 ABTS free radical Chemical class 0.000 description 5
- 206010013786 Dry skin Diseases 0.000 description 5
- 230000037336 dry skin Effects 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 230000003385 bacteriostatic effect Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 230000007794 irritation Effects 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 239000003755 preservative agent Substances 0.000 description 4
- 230000002335 preservative effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000002000 scavenging effect Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000110847 Kochia Species 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 229960002504 capsaicin Drugs 0.000 description 3
- 235000017663 capsaicin Nutrition 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 3
- 229960003957 dexamethasone Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 201000004624 Dermatitis Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108010002352 Interleukin-1 Proteins 0.000 description 2
- 102000000589 Interleukin-1 Human genes 0.000 description 2
- 244000042664 Matricaria chamomilla Species 0.000 description 2
- 235000007232 Matricaria chamomilla Nutrition 0.000 description 2
- 240000005546 Piper methysticum Species 0.000 description 2
- 235000016787 Piper methysticum Nutrition 0.000 description 2
- 241000304195 Salvia miltiorrhiza Species 0.000 description 2
- 235000011135 Salvia miltiorrhiza Nutrition 0.000 description 2
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000003042 antagnostic effect Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 150000002212 flavone derivatives Chemical class 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 210000000497 foam cell Anatomy 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 101710118538 Protease Proteins 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 244000007853 Sarothamnus scoparius Species 0.000 description 1
- 241000951376 Schizonepeta tenuifolia Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000191963 Staphylococcus epidermidis Species 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- 241001506047 Tremella Species 0.000 description 1
- 206010053648 Vascular occlusion Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000010523 cascade reaction Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000003837 chick embryo Anatomy 0.000 description 1
- 210000003711 chorioallantoic membrane Anatomy 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000013100 final test Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000003832 immune regulation Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000003960 inflammatory cascade Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008263 repair mechanism Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000009044 synergistic interaction Effects 0.000 description 1
- 208000021331 vascular occlusion disease Diseases 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/75—Anti-irritant
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Rheumatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Pain & Pain Management (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a soothing and repairing composition and a preparation method thereof, and belongs to the field of plant extraction research. The composition comprises purslane, fructus kochiae, duckweed, honeysuckle, dark plum and herba schizonepetae, and the skin problem induced by various factors such as environment, region, night, pressure, season change and the like is solved through the interaction of the components and multi-channel effect. The soothing and repairing composition has the effects of immediately relieving skin redness, pruritus and stabbing pain, removing free radicals, resisting inflammation, improving skin tolerance, repairing skin barriers and the like. It is applied in cosmetics and expected to have the effects of anti-irritation, skin soothing, antioxidation, anti-inflammation, skin barrier repair and the like.
Description
Technical Field
The invention relates to the field of plant extraction research, and particularly relates to a soothing and repairing composition as well as a preparation method and application thereof.
Background
With the rapid development of social urbanization and industrialization, environmental pollution and cosmetic stimulation cause more and more problems such as sensitive muscles, mask faces and hormone faces. Due to epidemic situations, people need to wear the mask every day, so that various skin problems are aggravated, and great troubles are brought to consumers both physically and psychologically. These skin problems have many similar symptoms, with the conditions of impaired skin barrier function, skin intolerance, skin inflammation, etc., and the repair mechanism is complex and requires multi-pathway synergy. Simply speaking, the skin-care product has the effects of repairing barriers, inhibiting bacteria, diminishing inflammation, relieving swelling and itching, improving skin tolerance and the like and simultaneously acts on the skin, the problem cannot be fundamentally solved by single-channel mechanism repair, the symptoms can be relieved only once, and the skin-care product is easy to repeatedly do.
Patent (CN 112137932A) discloses an anti-allergy restoration composition, which comprises Kochia SCOPARIA (Kochia SCOPARIA) extract, matricaria CHAMOMILLA (Chamomilla RECUTITA) flower extract, salvia MILTIORRHIZA (Salvia Miltiorrhiza) root extract, portulaca OLERACEA (Portulaca OLERACEA) extract, and Piper METHYSTICUM (Piper METHYSTICUM) leaf/root/stem extract. However, the composition only relieves the symptoms of the problem skin from the perspective of relieving the skin from redness, dryness, itching, burning, stinging, and the like.
Therefore, in view of the current situation that the action path is single and the skin problem cannot be fundamentally solved, it is very necessary to develop a plant extract which can relieve the problem skin through the synergistic action of multiple paths.
Disclosure of Invention
The invention mainly aims to provide a soothing and repairing composition and a preparation method thereof.
The soothing and repairing composition has the effects of immediately relieving skin redness, pruritus and stabbing pain, removing free radicals, resisting inflammation, improving skin tolerance, repairing skin barriers and the like.
Therefore, the technical scheme provided by the application is as follows:
in a first aspect, the present invention provides a soothing and repairing composition.
According to the invention, the soothing and repairing composition comprises the following components in parts by weight: 5-40 parts of purslane, 1-30 parts of fructus kochiae, 5-20 parts of duckweed, 1-20 parts of honeysuckle, 3-15 parts of dark plum and 1-10 parts of schizonepeta.
According to the present invention, the soothing repair composition is prepared by a preparation method comprising the steps of:
(1) Pretreating raw materials;
(2) Extracting the total raw materials and deionized water according to a certain ratio to obtain an extracting solution;
(3) Filtering the extracting solution in the step (2) to obtain a crude filtrate;
(4) Fine filtering the coarse filtrate obtained in the step (3) to obtain filtrate;
(5) And (5) sterilizing.
According to the invention, in step (2) of the preparation method, the weight ratio of the total weight of the raw materials to water is 1.
According to the invention, the weight ratio of the total weight of the starting materials to water in step (2) of the preparation process can be 1.
According to some embodiments of the invention, in step (2) of the preparation method, the weight ratio of the total weight of the raw materials to water is 1.
According to the invention, the extraction temperature in step (2) of the preparation method is 100-130 ℃.
According to some embodiments of the invention, the extraction temperature in step (2) of the preparation method is 110 ℃.
According to the invention, the extraction pressure in step (2) of the preparation method is 0.1-0.3MPa.
According to some embodiments of the invention, the extraction pressure in step (2) of the preparation process is 0.15MPa.
According to the invention, the extraction time in step (2) of the preparation method is 1-3h.
According to some embodiments of the invention, the extraction time in step (2) of the preparation method is 1.5h.
According to the invention, in the step (3) of the preparation method, the filter cloth is filtered by 60-100 meshes.
According to the invention, in the preparation method, the medicine residue is filtered off by rough filtration in the step (3).
According to the invention, the fine filtration mode in the step (4) of the preparation method is plate-and-frame filtration.
According to the invention, in the step (4), the aperture of the fine filter plate frame filter plate is 0.3-0.5 μm.
According to the invention, in the step (4), the fine filter plate frame filter plate is SCP-1120 type.
According to the present invention, the sterilization mode in the step (5) of the production method is a conventional sterilization mode.
According to the invention, the sterilization mode in the step (5) of the preparation method is high-temperature sterilization.
According to some embodiments of the invention, the sterilization temperature in step (5) of the preparation method is 90-95 ℃.
According to some embodiments of the invention, the sterilization time in step (5) of the preparation method is 30-60min.
According to some embodiments of the invention, step (5) of the preparation process further comprises adding a preservative.
According to some embodiments of the invention, the preservative added in step (5) of the preparation method is pentanediol or hexanediol.
According to some embodiments of the invention, in step (5), the pentanediol is added in an amount of 1 to 5wt% and the hexanediol is added in an amount of 0.5 to 2wt%, based on the weight of the filtrate.
According to the invention, the raw material pretreatment in the step (1) of the preparation method comprises foreign matter picking and cleaning.
In a second aspect, the present invention provides a method of preparing a soothing and repairing composition according to the first aspect of the present invention.
According to the present invention, the method of preparing the soothing and repairing composition comprises the steps of:
(1) Pretreating raw materials, namely weighing 5-40 parts of purslane, 1-30 parts of fructus kochiae, 5-20 parts of duckweed, 1-20 parts of honeysuckle, 3-15 parts of dark plum and 1-10 parts of schizonepeta;
(2) Extracting the total raw materials and deionized water according to a material-liquid ratio of 1;
(3) Filtering the extracting solution obtained in the step (2) by using 60-100 meshes of filter cloth to obtain a crude filtrate;
(4) Finely filtering the coarse filtrate obtained in the step (3) by using a plate-and-frame filtration mode, wherein the aperture of a filter plate is 0.3-0.5 mu m, and obtaining filtrate;
(5) And (5) sterilizing.
According to the invention, the weight ratio of the total weight of the raw material to water in step (2) of the preparation process may be 1.
According to some embodiments of the present invention, in step (2) of the preparation method, the weight ratio of the total weight of the raw materials to water is 1.
According to some embodiments of the invention, the extraction temperature in step (2) of the preparation method is 110 ℃.
According to some embodiments of the invention, the extraction pressure in step (2) of the preparation process is 0.15MPa.
According to some embodiments of the invention, the extraction time in step (2) of the preparation method is 1.5h.
According to the invention, in the preparation method, the medicine residue is filtered off by rough filtration in the step (3).
According to the invention, the fine filtration mode in the step (4) of the preparation method is plate-frame filtration.
According to the invention, in the step (4), the fine filter plate frame filter plate is SCP-1120 type.
According to the present invention, the sterilization mode in the step (5) of the production method is a conventional sterilization mode.
According to the invention, the sterilization mode in the step (5) of the preparation method is high-temperature sterilization.
According to some embodiments of the invention, the sterilization temperature in step (5) of the preparation method is 90-95 ℃.
According to some embodiments of the invention, the sterilization time in step (5) of the preparation method is 30-60min.
According to some embodiments of the invention, step (5) of the preparation process further comprises adding a preservative.
According to some embodiments of the invention, the preservative added in step (5) of the preparation method is pentanediol, hexanediol.
According to some embodiments of the invention, in step (5), the pentanediol is added in an amount of 1 to 5wt% and the hexanediol is added in an amount of 0.5 to 2wt%, based on the weight of the filtrate.
According to the invention, the raw material pretreatment in the step (1) of the preparation method comprises foreign matter picking and cleaning.
In a third aspect, the present invention provides the use of a soothing and repairing composition according to the first aspect of the present invention and/or a soothing and repairing composition prepared according to the method of the second aspect of the present invention in the cosmetic and/or dermatological field.
According to the invention, the skin care product is a facial mask, a cream, an emulsion, a skin lotion, a facial cleanser, a shampoo, a hair conditioner or a body wash and the like, and is not limited by dosage forms.
The invention has the advantages of
(1) The soothing and repairing composition comprises six functional components of purslane, fructus kochiae, duckweed, honeysuckle, dark plum and herba schizonepetae, has a multi-channel synergistic effect, and solves the skin problems such as sensitive muscles induced by various factors such as environment, regions, night, pressure and season change through the effects of 'antagonistic stimulation, soothing skin, removing free radicals, resisting inflammation, inhibiting bacteria, repairing skin barrier, improving skin tolerance' and the like.
(2) The invention adopts a high-temperature high-pressure extraction process, improves the content of functional components such as total sugar, total flavone and polyphenol in the soothing and repairing composition, promotes the expression of the functional components, accelerates the reaction process, improves the utilization rate of raw materials and saves the production cost.
Drawings
FIG. 1 is a graph showing the results of capsaicin challenge experiments;
FIG. 2 is a score chart of scratchiness after lactic acid stimulation in example 1, example 7, comparative example 3 to comparative example 11;
FIG. 3 is a graph of the assessment of the stinging sensation after lactic acid stimulation for examples 1, 7, 3-11;
FIG. 4 is a graph of the assessment of the sting scores after lactic acid stimulation for examples 1, 7, 19-20;
FIG. 5 is a graph of the assessment of scratchiness after lactic acid stimulation of example 1, example 7, comparative example 19 to comparative example 20;
FIG. 6 is a graph of ABTS free radical clearance.
Detailed Description
The present invention will be described in detail below with reference to the drawings and examples, but the present invention is not limited to the examples.
The sources of the raw materials used in the present invention are shown in Table 1, and the raw materials and the apparatuses used in the present invention are conventional raw materials or apparatuses unless otherwise specified.
TABLE 1 use of raw material sources
Example 1: weighing 30g of purslane, 20g of fructus kochiae, 15g of duckweed, 10g of honeysuckle, 10g of dark plum and 5g of herba schizonepetae; 4500g of deionized water is added according to the feed-liquid ratio of 1; extracting at 110 deg.C under 0.15MPa for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Example 2: weighing 5g of purslane, 1g of fructus kochiae, 5g of duckweed, 1g of honeysuckle, 3g of dark plum and 1g of herba schizonepetae; adding 1120g of deionized water according to the feed-liquid ratio of 1; extracting at 100 deg.C under 0.1MPa for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Example 3: weighing 40g of purslane, 30g of fructus kochiae, 20g of duckweed, 20g of honeysuckle, 15g of dark plum and 10g of schizonepeta; adding 2700g of deionized water according to the feed-liquid ratio of 1; extracting at 130 deg.C under 0.3MPa for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Example 4: weighing 30g of purslane, 20g of fructus kochiae, 15g of duckweed, 10g of honeysuckle, 10g of dark plum and 5g of herba schizonepetae; adding 1800g of deionized water according to the feed-liquid ratio of 1; extracting at 110 deg.C under 0.15MPa for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Example 5: weighing 30g of purslane, 20g of fructus kochiae, 15g of duckweed, 10g of honeysuckle, 10g of dark plum and 5g of schizonepeta; 4500g of deionized water is added according to the feed-liquid ratio of 1; extracting at 100 deg.C under 0.1MPa for 1.5h; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Example 6: weighing 30g of purslane, 20g of fructus kochiae, 15g of duckweed, 10g of honeysuckle, 10g of dark plum and 5g of herba schizonepetae; adding 1800g of deionized water according to the feed-liquid ratio of 1; extracting at 130 deg.C under 0.3MPa for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Example 7: weighing 20g of purslane, 20g of fructus kochiae, 15g of duckweed, 10g of honeysuckle, 5g of dark plum and 5g of schizonepeta; 3750g of deionized water is added according to the feed-liquid ratio of 1; extracting at 110 deg.C under 0.15MPa for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Comparative example 1
Weighing 30g of purslane, 20g of fructus kochiae, 15g of duckweed, 10g of honeysuckle, 10g of dark plum and 5g of herba schizonepetae, and adding 4500g of deionized water according to a material-liquid ratio of 1; extracting in water bath at 80 deg.C for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Comparative example 2
Weighing 30g of purslane, 20g of fructus kochiae, 15g of duckweed, 10g of honeysuckle, 10g of dark plum and 5g of herba schizonepetae, and adding 4500g of deionized water according to a material-liquid ratio of 1; extracting at 150 deg.C under 0.5MPa for 1.5h; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Comparative examples 3 to 11
Preparing extracting solutions of comparative examples 3-11 according to the raw materials and the processes of the table 2 respectively; then filtering the extract once by using 100-mesh filter cloth, and then filtering by using a 0.3-0.5 mu m filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
Table 2 comparative examples 3 to 11 raw material composition ratios and preparation processes
Comparative examples 12 to 17
Preparing extracting solutions of comparative examples 12 to 17 according to the raw materials and the processes of the table 3 respectively; then filtering the extract once by using 100-mesh filter cloth, and then filtering by using a filter plate with the diameter of 0.3-0.5 mu m; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain the composition.
TABLE 3 raw material composition ratios and preparation processes for comparative examples 12 to 17
Comparative example 18
According to comparative example 12: comparative example 13: comparative example 14: comparative example 15: comparative example 16: comparative example 17 extract =6:4:4:2:2:1 taking 4500g of the extract, and mixing and stirring uniformly to obtain a mixed solution.
Comparative example 19
Weighing 1g of purslane, 1g of fructus kochiae, 1g of duckweed, 20g of honeysuckle, 20g of dark plum and 20g of herba schizonepetae, and adding 3150g of deionized water according to a material-liquid ratio of 1; extracting at 110 deg.C under 0.15MPa for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain extract.
Comparative example 20
Weighing 5g of purslane, 5g of fructus kochiae, 5g of duckweed, 5g of honeysuckle, 20g of dark plum and 20g of herba schizonepetae, and adding 3000g of deionized water according to a material-liquid ratio of 1; extracting at 110 deg.C under 0.15MPa for 1.5 hr; filtering the extractive solution with 100 mesh filter cloth, and filtering with 0.3-0.5 μm filter plate; adding 2% pentanediol and 1.5% hexanediol for antisepsis, and sterilizing in water bath at 90-95 deg.C for 30min to obtain extract.
Test example 1: basic index test of plant composition by different preparation processes
1.1 Determination of polysaccharide and total sugar content
The method is carried out by adopting a phenol-sulfuric acid method (specifically, refer to the content determination of polysaccharide in tremella [ J ]. Heilongjiang medical science 1999 (06): 32-32.).
The value: measured with a pH meter.
Solid content: measured by a solid content instrument.
In order to prevent the influence of concentration multiple error on the content of the effective components of the extract, all indexes are measured as filtrate before concentration.
TABLE 4 basic indices of the plant compositions for the different preparation processes
When the formula composition is prepared by a water extraction method, some light medicinal materials are found to float on the surface of the liquid, and the problem cannot be solved even if the medicinal materials are stirred, so that the medicinal materials are easy to react insufficiently, and the extraction efficiency is greatly reduced. The high temperature and high pressure method is a method for extracting active ingredients under a closed condition with higher pressure intensity, the extraction method can accelerate reaction during reaction, and meanwhile, the closed condition can ensure that water vapor can fully contact each medicinal material, so that the reaction efficiency is improved. As can be seen from Table 4, the high temperature and high pressure treatment improves the total sugar content, total flavone content and polyphenol content of the composition, avoids resource waste caused by insufficient utilization of raw materials, and greatly saves cost, compared with the common water extraction method. Meanwhile, by comparing examples 1, 5-6 and 2, it can be seen that when the temperature and pressure of the high-temperature high-pressure treatment exceed a certain range, the higher temperature and pressure basically do not improve the extraction efficiency of the plants, but the too high pressure not only brings about a potential safety hazard, but also increases energy consumption and causes energy waste. In summary, the high-temperature and high-pressure extraction conditions of the invention are that the extraction pressure is 0.1-0.3MPa and the extraction temperature is 100-130 ℃.
TABLE 5 basic indices of extracts of single and combined materials
| pH value | Solids content/% | Color of solution | |
| Example 1 (high temperature and high pressure method) | 4.98 | 2.8 | Dark brown color |
| COMPARATIVE EXAMPLE 12 (purslane) | 5.85 | 1.2 | Earthy yellow |
| COMPARATIVE EXAMPLE 13 (Kochia scoparia) | 5.81 | 1.0 | Brown colour |
| COMPARATIVE EXAMPLE 14 (Duckweed) | 6.10 | 1.0 | Dark brown color |
| COMPARATIVE EXAMPLE 15 (honeysuckle) | 5.29 | 1.6 | Yellow colour |
| COMPARATIVE EXAMPLE 16 (smoked plum) | 2.62 | 1.6 | Brown colour |
| COMPARATIVE EXAMPLE 17 (Schizonepeta tenuifolia) | 5.63 | 0.4 | Light yellow |
| Comparative example 18 (Mixed solution) | 5.21 | 1.3 | Black brown color |
The traditional Chinese medicine is decocted into a single decoction and a repeated decoction, wherein the repeated decoction is to boil all the medicinal materials in a pot at the same time, and the single decoction is to boil each medicinal material respectively and then mix the decoctions. To verify the effect of single and multiple simmering on the efficacy of the formula, the data of the relevant indices of example 1 and comparative examples 12-17 were tested and the results are shown in table 5. Compared with the comparative example 18, the solution in the example 1 has similar color and higher solid content, which indicates that certain weak interaction occurs between the medicinal materials during re-decoction, a supramolecular chemical phenomenon is generated, and functional components are promoted to be dissolved in the extracting solution. Because the pH value (2.62) of the dark plum extract is too low, the dark plum extract is easy to cause irritation to skin, and the dark plum extract is abandoned in later experiments.
Capsaicin test
Test samples: example 1, comparative examples 12 to 18
Testing part: the inner sides of the left and right arms;
selecting the inner sides of the left and right arms of a subject as tested parts, and circularly marking the tested parts in a square area with the specification of 2cm multiplied by 2 cm; cleaning a tested area (the same area) by using clean water, standing the tested area for 10min at the temperature of 20.6-21.2 ℃ and the relative humidity of 43-52%, measuring a blank value of a skin red value by using a Mexamter MX18 (a pigment testing probe) of Germany CK company, and then entering a coating experiment stage; stimulating the tested area with 0.02% capsaicin aqueous solution (about 0.01 mL); continuously stimulating for 15min and 30min, and collecting numerical value; then smearing a test sample (5 wt% solution, about 0.05 mL) on a tested area for 15min, 30min and 45min, and then performing data acquisition; smearing 0.05mL of pure water on the blank control; coating the positive control group with 0.2wt% Symcalmin Dermax aqueous solution; counting the value measured at each time of the experimental part of the subject, and analyzing the change of the red value.
The change of the skin red value is reflected in the change rule of the skin redness of the experimental area along with the time in the testing period. The greater the value, the greater the degree of redness of the skin. Skin red value change rate (%) = (T-T blank)/T blank × 100%.
As can be seen from fig. 1, example 1 has a remarkable effect of repairing skin after irritation, and rapidly reduces the redness of skin caused by irritation, thereby repairing damaged skin. Comparative example 18 (mixed solution) and comparative example 15 (honeysuckle flower) have certain repairing effect, but the repairing effect is not as good as that of example 1. This is because during the re-decoction process, some weak interaction between the herbs can occur, which can generate supermolecule chemical phenomena to promote the efficacy of the active ingredients.
Test example 2: comparison of efficacy of extracts of formulas at different ratios
Test samples: example 1, example 7, comparative examples 3-11 and comparative examples 19-20, samples were diluted to 5% aqueous solutions.
Testing the population: 120 (18-55 years old, male and female unlimited) volunteers were selected and divided into 12 groups. 10 people per group.
The lactic acid stimulation method was carried out by applying filter paper (about 0.8cm in diameter) soaked with 50. Mu.L of 10% lactic acid solution to the nasolabial folds of both sides of the volunteers for 10min and removing the filter paper. A tester coats samples on test areas on two sides simultaneously, two samples in the same group are randomly distributed on two sides, the sample coating amount of each area is 0.1mL, before and after the samples are used, the samples are 0min, 0.5min, 2min, 5min, 10min and 15min, and a volunteer subjectively evaluates itching feeling and prickling feeling at nasolabial folds on two sides (the total score is 5 points, the more the itching feeling and the prickling feeling are higher, 0-1 points represent no feeling or a little feeling, 1-3 points represent mild stimulation, the itching feeling and the prickling feeling can be felt, 3-5 points represent severe stimulation, and the itching feeling and the prickling feeling are serious). The test samples were evaluated for immediate tingling and itching relief using sensory evaluation of volunteers, with experimental results as shown in fig. 2-5.
As can be seen from fig. 2 to 3, the examples and comparative examples each had the effect of instantly relieving itching and stinging after using the samples. The instant itching and stinging relieving effect of the soothing and repairing composition prepared by combining any five or three raw materials in the compositions in the comparative examples 3-11 is obviously better than the instant itching and stinging relieving effect of the soothing and repairing composition prepared by combining any five or three raw materials in the compositions in the comparative examples 1-11, which shows that the six components of the composition have a synergistic effect, and if one raw material or a plurality of raw materials are removed, the effect of the soothing and repairing composition is greatly reduced.
From FIGS. 4 to 5, however, example 1 had better immediate relief of itching and stinging than comparative examples 19 to 20, indicating that the ratios of the components of the formula may have varying degrees of effect. The embodiment of the invention is the optimal component proportion obtained by multiple experimental screening, because the intermediate experimental process is too much and cannot be put on one by one, and the lists are typical embodiments and comparative examples.
In conclusion, the composition formula, the proportion and the preparation process can influence the effect expression of the composition, and six effect components of purslane, fructus kochiae, honeysuckle, duckweed, dark plum fruit and schizonepeta in the composition have synergistic effect through multiple channels, so that unexpected technical effects are generated.
Test example 3: efficacy verification
3.1 scavenging of free radicals
Test samples: example 1, comparative example 8, comparative example 9, comparative example 20.
The experimental method comprises the following steps: scavenging free radicalsThe experimental method is as follows: preparation is 7 mmol.L -1 ABTS stock solution and 140 mmol. L -1 Potassium persulfate stock solution. A certain amount of 7 mmol. Multidot.L -1 ABTS and 140 mmol. L -1 Mixing potassium persulfate, and standing at room temperature in a dark place for a period of time to form ABTS free radical stock solution. A certain amount of ABTS free radical stock solution is taken and diluted by 50% ethanol, so that the light absorption value of the final test negative control is 0.70 +/-0.02.
Free radicals are a very active class of reactive molecular species (often called oxygen radicals because most of them contain free oxygen atoms, if not otherwise stated, free radicals are products of human metabolism, and environmental factors can also increase free radicals, such as pollution, ultraviolet light, and sunlight. Harmfulness of free radicals to the human body: 1. the electrons of the cell membrane are easily lost, so the cell membrane is extremely vulnerable to free radicals, and once the electrons are abstracted by the free radicals, the cell membrane loses elasticity and loses part or all functions, thereby causing cardiovascular diseases (the free radicals are generally considered to be the root cause of all diseases and aging). More seriously, the attack of free radicals to genes can damage the molecular structure of the genes in a cup, so that the genes are mutated, and the whole life is systematically disordered; 2. bad cholesterol in the human body is easily oxidized by free radicals, and after oxidation, foam cells are formed, and the foam cells can be attached to the blood vessel wall. After a certain amount of time has elapsed, the blood vessel is blocked or broken into fragments and the fragments are introduced into the blood, and the blood is coagulated to form thrombus when contacting the fragments. Thrombosis can cause vascular occlusion, a coronary disease if it occurs in the coronary arteries supplying the heart's blood, and a stroke if it occurs in the brain. That is, the chief culprit in atherosclerosis is the oxidation of these free radicals by bad cholesterol; 3. free radicals act on the human endoprotease system, resulting in the release of collagenase and scleroelastase, which act on collagen and scleroelastin in the skin and cause them to undergo excessive cross-linking and degradation, with consequent loss of elasticity and the appearance of wrinkles.
The soothing and repairing composition of the invention has good effect of scavenging free radicals, and as shown in fig. 6, the scavenging rate of the extract of example 1 on free radicals can reach 100% when the concentration is 0.5% and 2.0%. The clearance rate of the soothing and repairing composition of example 1 of the invention on free radicals is higher than that of the soothing and repairing composition obtained in comparative example 8, comparative example 9 and comparative example 20, which shows that the combination of six effective components of purslane, fructus kochiae, honeysuckle, duckweed, dark plum and herba schizonepetae of the invention produces obvious synergistic interaction, and different components promote each other, thus having better effect of clearing free radicals.
Repairing barrier and relieving swelling
The experimental method comprises the following steps: 100 skin pruritus, red swelling and dry desquamation volunteers are recruited and divided into 4 groups of 25 people, and after normal cleaning and skin care every day, the test samples of example 1, comparative example 8, comparative example 9 and comparative example 20 (all 5% aqueous solution) are respectively smeared and used for 7 days.
Table 6 example 1 composition improves skin effects
| Improving skin itch | Improving skin redness and swelling | Improving dry skin desquamation | |
| Use for 15 minutes | 24/25 human, 96% | 2/25 human, 8% | 1/25 human, 4% |
| It can be used for 1-3 |
25/25 people, 100% | 20/25 human, 80% | 18/25 human, 72% |
| It is used for 7 |
25/25 human, 100% | 25/25 human, 100% | 25/25 human, 100% |
Table 7 skin improvement effect of comparative example 8 composition
| Improving skin itch | Improving skin redness and swelling | Improving dry skin desquamation | |
| Use for 15 |
5/25 human, 20% | 0/25 human, 0% | 2/25 human, 20% |
| It can be used for 1-3 days | 12/25 human, 48% | 3/25 human, 12% | 10/25 human, 40% |
| Use 7Sky | 21/25 human, 84% | 7/25 human, 28% | 20/25 human, 80% |
Table 8 skin improving effect of comparative example 9 composition
| Improving skin itch | Improving skin redness and swelling | Improving dry skin desquamation | |
| Use for 15 |
3/25 human, 12% | 0/25 human, 0% | 2/25 human, 8% |
| It can be used for 1-3 |
5/25 human, 20% | 2/25 human, 8% | 5/25 of human body, 20% |
| It can be used for 7 days | 12/25 human, 48% | 8/25 human, 32% | 13/25 human, 52% |
Table 9 the composition of comparative example 20 improved skin effects
| Improving skin itch | Improving skin redness and swelling | Improving dry skin desquamation | |
| Use for 15 minutes | 13/25 human, 52% | 0/25 human, 0% | 3/25 human, 20% |
| It can be used for 1-3 days | 18/25 human, 72% | 4/25 human, 16% | 9/25 human, 36% |
| It can be used for 7 |
20/25 human, 80% | 11/25 human, 44% | 14/25 human, 56% |
The volunteers can improve the skin pruritus feeling by applying the paint in example 1 for 15min, and the skin pruritus feeling is up to 96%, while the effect of the comparative example is obviously lower, and the highest improvement rate is only 52%. After 1-3 days of use, most of the volunteers of example 1 improved the redness and swelling of the skin up to 80%, while the improvement effect of the comparative example did not exceed 16%. The dry skin peeling phenomenon of 75% of volunteers was improved after 1 to 3 days using example 1, while the improvement effect of the comparative example was not more than 40%. The symptoms of itching, redness and swelling, and dry desquamation of the skin of 100% of volunteers 7 days after using example 1 can be improved, while the improvement effect of the comparative example is obviously inferior to that of example 1. And the volunteer feedback of example 1 was used, and then the skin allergy phenomenon was reduced and the feeling was not so stimulated when other products were used, which also indicates that the formulation has a good barrier repair effect. While the other groups used the volunteers of the comparative example, without feedback that the skin barrier function was improved. The invention is prepared from purslane, broom cypress fruit, honeysuckle flower, duckweed, dark plum fruit the combination of six effective components of the schizonepeta generates obvious synergistic and synergistic effect, can better relieve the symptoms of skin itch, red swelling, dryness and desquamation, and has the effect of repairing skin barrier.
In order to better verify other effects of the formula, the anti-inflammatory and antibacterial effect detection is further carried out on the soothing and repairing composition.
Anti-inflammatory assay
Lipopolysaccharide (LPS) is adopted for induction, an action object is RAW264.7, general body inflammation is simulated, and a tested inflammatory factor is IL-1 beta. IL-1 beta plays an important role in immune regulation and inflammatory reaction processes, is considered to be one of main endogenous mediators causing fever reaction in the inflammatory reaction process, and once the content of inflammatory factors in skin cells is increased and the skin temperature is higher than normal temperature, the skin inflammatory reaction is intensified to trigger the body inflammatory cascade reaction.
Test samples: example 1, comparative example 8, comparative example 20;
the experimental method comprises the following steps: anti-inflammatory assays were performed using LPS-induced RAW264.7 cell inflammation model:
(1) Inoculation: inoculating cells into 24-well plates, 37 ℃,5% 2 The incubator is incubated for 18-24h.
(2) Preparing liquid: the test substances and positive controls were formulated according to table 10.
(3) Sample feeding: grouping and concentration setting were tested according to table 10, and samples were given in groups of 3 replicate wells per treatment group after 18-24h of cell plating growth in 24-well plates. In group 1, the cell culture medium containing 0.1% dmso was added to both the blank control and the negative control, and the cell culture medium containing 0.001% dexamethasone was added to the positive control; in group 2, the blank control and the negative control were added with cell culture medium, and the sample group was added with cell culture medium containing the corresponding concentration of sample, at 37 ℃ and 5% CO 2 The incubator continues to culture for 18-24h.
Table 10 experimental groupings and concentration settings
(4) And (3) LPS induction: after culturing for 24h, sucking out the culture medium in the plate, adding PBS for light washing once, adding cell culture medium into blank control group, adding cell culture medium containing LPS into negative control group, sample group and positive control group, and culturing at 37 deg.C with 5% CO 2 The incubator continues to culture for 24h and then the supernatant is collected.
(5) Detecting the content of IL-1 beta: cell supernatants from each well were taken and assayed for the content of the cytokine IL-1. Beta. According to the ELISA kit protocol, the results are shown in Table 11.
TABLE 11 detection results of IL-1. Beta. Expression of inflammatory factor
LPS induction (LPS +) caused a significant increase in the expression of the inflammatory factor IL-1 β compared to the BC (LPS-) group: (P<0.01 Indicating that LPS induces molding successfully; dexamethasone in the PC (dexamethasone) group, at 0.001% dosing concentration, significantly down-regulated the amount of IL-1 β expression compared to SC (0.1% DMSO, LPS +), (P<0.01 And the result shows that the detection result is valid.
The effects of the test results of the expression of the inflammatory factor IL-1 beta of the soothing and repairing composition are shown in Table 11, and compared with NC (LPS +) group, the soothing and repairing composition has significant inhibition effects on the expression of the inflammatory factor IL-1 beta within the concentration ranges of 0.08%, 0.16% and 0.31%; at a concentration of 0.08%, the soothing and repairing composition of the invention has a significant inhibitory effect on the expression of the inflammatory factor IL-1 β, compared to comparative example 8 and comparative example 20.
Bacteriostatic effect
The antibacterial effect of the soothing and repairing composition is detected according to the evaluation method of the antibacterial and bacteriostatic effects of QB/T2738-2012 daily chemical products.
TABLE 12 bacteriostatic effect of soothing and repairing compositions of the present invention
As shown in Table 12, the soothing and repairing composition has a good bacteriostatic effect on Staphylococcus epidermidis and Staphylococcus aureus.
Safety feature
In order to ensure the safety of the formula, a chick embryo chorioallantoic membrane test and a rabbit corneal epithelial cell in-vitro short-time exposure test are carried out, and the test result shows that the composition has no irritation. The result of the erythrocyte hemolytic blood coagulation test shows that the blood coagulation phenomenon does not occur.
In conclusion, the soothing and repairing composition comprises six functional components of purslane, fructus kochiae, duckweed, honeysuckle, dark plum and herba schizonepetae, has a multi-channel synergistic effect, and solves the skin problems, such as sensitive muscles, induced by various factors such as environment, regions, night-out, pressure and season change through the effects of 'antagonistic stimulation, skin soothing, free radical removal, anti-inflammation, bacteriostasis, skin barrier repairing, skin tolerance improvement and the like'. In terms of safety, has very reliable safety, and can be safely applied to the field of cosmetics.
The above examples are given for the purpose of illustrating the invention clearly and not for the purpose of limiting the same, and it will be apparent to those skilled in the art that, in light of the foregoing description, numerous modifications and variations can be made in the form and details of the embodiments of the invention described herein, and it is not intended to be exhaustive or to limit the invention to the precise forms disclosed.
Claims (6)
1. The soothing and repairing composition is characterized by comprising the following raw materials in parts by weight: 5-40 parts of purslane, 1-30 parts of fructus kochiae, 5-20 parts of duckweed, 1-20 parts of honeysuckle, 3-15 parts of dark plum and 1-10 parts of schizonepeta;
the soothing repair composition is prepared by a preparation method comprising the following steps:
(1) Pretreating raw materials;
(2) Extracting the total raw materials and deionized water according to a certain material-liquid ratio to obtain an extracting solution;
(3) Filtering the extracting solution obtained in the step (2) to obtain a crude filtrate;
(4) Fine filtering the coarse filtrate obtained in the step (3) to obtain filtrate;
(5) Sterilizing;
the weight ratio of the total weight of the raw materials in the step (2) of the preparation method to the water is 1; in the preparation method, in the step (2), the extraction temperature is 100-130 ℃, the extraction pressure is 0.1-0.3MPa, and the extraction time is 1-3h.
2. A soothing servicing composition according to claim 1, wherein the filtration cloth in step (3) of the preparation method is 60-100 mesh.
3. A soothing and repairing composition as claimed in claim 2, wherein the fine filtration in step (4) of the preparation method is plate-and-frame filtration, and the pore size of the filter plate is 0.3-0.5 μm.
4. A method of preparing a soothing and repairing composition, comprising the steps of:
(1) Pretreating raw materials, weighing 5-40 parts of purslane, 1-30 parts of fructus kochiae, 5-20 parts of duckweed, 1-20 parts of honeysuckle, 3-15 parts of dark plum and 1-10 parts of schizonepeta as total raw materials;
(2) Extracting the total raw materials and deionized water according to a weight ratio of 1-1;
(3) Filtering the extracting solution obtained in the step (2) by using 60-100-mesh filter cloth to obtain a crude filtrate;
(4) Finely filtering the coarse filtrate obtained in the step (3) by using a plate-and-frame filtration mode, wherein the aperture of a filter plate is 0.2-10 mu m, and obtaining filtrate;
(5) And (5) sterilizing.
5. Use of a soothing rejuvenating composition according to any one of claims 1 to 3 or prepared according to the process for preparing a soothing rejuvenating composition according to claim 4 for the preparation of a skin care product.
6. Use according to claim 5, wherein the soothing and repairing composition is used for preparing a skin care product with skin soothing, free radical scavenging, anti-inflammatory, skin tolerance improving or skin barrier repairing effects.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110305740.6A CN113181089B (en) | 2021-03-23 | 2021-03-23 | Soothing and repairing composition and preparation method and application thereof |
| PCT/CN2022/081927 WO2022199510A1 (en) | 2021-03-23 | 2022-03-21 | Alleviating and repairing composition, and preparation method therefor and use thereof |
| KR1020237034944A KR20230149856A (en) | 2021-03-23 | 2022-03-21 | Composition for palliative recovery and method of manufacturing and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110305740.6A CN113181089B (en) | 2021-03-23 | 2021-03-23 | Soothing and repairing composition and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113181089A CN113181089A (en) | 2021-07-30 |
| CN113181089B true CN113181089B (en) | 2022-10-11 |
Family
ID=76973625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110305740.6A Active CN113181089B (en) | 2021-03-23 | 2021-03-23 | Soothing and repairing composition and preparation method and application thereof |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR20230149856A (en) |
| CN (1) | CN113181089B (en) |
| WO (1) | WO2022199510A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113181089B (en) * | 2021-03-23 | 2022-10-11 | 太和康美(北京)中医研究院有限公司 | Soothing and repairing composition and preparation method and application thereof |
| CN113244151B (en) * | 2021-05-31 | 2022-07-29 | 植然天成(北京)生物科技有限公司 | Soothing repair cream and preparation method thereof |
| CN113318166B (en) * | 2021-06-30 | 2022-10-28 | 植然天成(北京)生物科技有限公司 | Plant composition and preparation method and application thereof |
| CN114533612A (en) * | 2022-02-17 | 2022-05-27 | 广东芭薇生物科技股份有限公司 | Composition with effects of relieving, removing red and repairing and application thereof |
| CN116270350B (en) * | 2023-03-24 | 2024-03-29 | 北京工商大学 | Flower composition and preparation method and application thereof |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050176677A1 (en) * | 2002-04-30 | 2005-08-11 | Claude Dal Farra | Cosmetic composition comprising monosaccharides or polysaccharides, uses and treatment methods |
| KR20130035408A (en) * | 2011-09-30 | 2013-04-09 | (주)아모레퍼시픽 | Composition for reducing skin irritation comprising prunus mume extracts |
| WO2017051950A1 (en) * | 2015-09-24 | 2017-03-30 | 노병승 | Method for manufacturing oriental medicinal soap for improving atopic dermatitis |
| CN105168661A (en) * | 2015-10-12 | 2015-12-23 | 临沂草之美医药科技有限公司 | Traditional Chinese medicine liniment for treating skin itch and preparation method thereof |
| CN112137921A (en) * | 2019-06-27 | 2020-12-29 | 东莞圣幸健康科技有限公司 | Soothing and anti-allergy composition, application thereof and soothing and anti-allergy mask |
| CN111419751A (en) * | 2020-04-23 | 2020-07-17 | 广州市科能化妆品科研有限公司 | Soothing composition, preparation method thereof and skin care product containing soothing composition |
| CN113181089B (en) * | 2021-03-23 | 2022-10-11 | 太和康美(北京)中医研究院有限公司 | Soothing and repairing composition and preparation method and application thereof |
| CN113244151B (en) * | 2021-05-31 | 2022-07-29 | 植然天成(北京)生物科技有限公司 | Soothing repair cream and preparation method thereof |
| CN113318166B (en) * | 2021-06-30 | 2022-10-28 | 植然天成(北京)生物科技有限公司 | Plant composition and preparation method and application thereof |
-
2021
- 2021-03-23 CN CN202110305740.6A patent/CN113181089B/en active Active
-
2022
- 2022-03-21 KR KR1020237034944A patent/KR20230149856A/en not_active Application Discontinuation
- 2022-03-21 WO PCT/CN2022/081927 patent/WO2022199510A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| CN113181089A (en) | 2021-07-30 |
| KR20230149856A (en) | 2023-10-27 |
| WO2022199510A1 (en) | 2022-09-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113181089B (en) | Soothing and repairing composition and preparation method and application thereof | |
| CN108815072B (en) | Fresh plant composition fermented raw pulp with moisturizing and relieving effects and preparation method and application thereof | |
| CN105395440B (en) | A kind of peach gum facial mask and its preparation method and application | |
| KR101189989B1 (en) | Cosmetic composition containing as available ingredient the extracts of Equisetum arvense L. | |
| CN112754956A (en) | Formula and preparation method of cactus polysaccharide anti-inflammatory moisturizing mask | |
| CN104287996A (en) | Eucommia shampoo and preparing method thereof | |
| CN112336641A (en) | Hypochlorous acid hand-washing-free disinfectant and preparation method thereof | |
| CN110787119A (en) | Antibacterial skin-care traditional Chinese medicine composition and preparation method and application thereof | |
| CN106974858A (en) | A kind of acne-removing composition of Sage Extract and its application | |
| CN110613663A (en) | Skin repairing composition, skin care product and preparation method thereof | |
| CN110755340A (en) | A skin care bath lotion containing natural plant extract components and its preparation method | |
| CN112933018B (en) | Composition with barrier repairing and anti-photoaging effects and preparation method and application thereof | |
| CN112494408B (en) | Whitening and inflammation diminishing mask liquid | |
| CN110327266B (en) | Amino acid shampoo with functions of inhibiting bacteria, relieving itching and protecting hair and repairing | |
| CN110237017B (en) | Toning lotion containing ledum tenuifolia essential oil and preparation method thereof | |
| CN107550810B (en) | Traditional Chinese medicine composition and preparation method and application thereof | |
| CN113576970A (en) | Anti-aging plant extract composition and preparation and application thereof | |
| CN112807263A (en) | Anti-aging composition and preparation method and application thereof | |
| CN112206177A (en) | Plant essence and preparation method thereof | |
| KR20120043254A (en) | Cosmetic pack composition containing jeju scoria powder | |
| CN112300877A (en) | Formula and preparation method of traditional Chinese medicine soap | |
| CN115463065B (en) | Plant composition with anti-allergy relieving effect and preparation method and application thereof | |
| CN114869818B (en) | Composition with repairing effect and preparation method thereof | |
| CN115006328B (en) | Whitening and freckle-removing composition, and preparation method and application thereof | |
| CN110577864B (en) | Skin-moistening soap prepared by taking fish oil as animal fat |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230615 Address after: 102401 80701, 7th floor, education practice building, 11 Xueyuan North Street, Gongchen sub district office, Fangshan District, Beijing Patentee after: TAIHE KANGMEI (BEIJING) TRADITIONAL CHINESE MEDICINE INSTITUTE Co.,Ltd. Patentee after: NUTRI-WOODS BIO-TECH (BEIJING) Co.,Ltd. Address before: 102488 80701, 7th floor, education practice building, 11 Xueyuan North Street, Gongchen sub district office, Fangshan District, Beijing Patentee before: TAIHE KANGMEI (BEIJING) TRADITIONAL CHINESE MEDICINE INSTITUTE Co.,Ltd. |
|
| TR01 | Transfer of patent right |