CN113171319B

CN113171319B - Instant and long-acting anti-aging wrinkle-removing compound plant extract and preparation method and application thereof

Info

Publication number: CN113171319B
Application number: CN202110496488.1A
Authority: CN
Inventors: 刘露; 刘逸华; 贺青
Original assignee: Baocui Biotechnology Co ltd
Current assignee: Baocui Biotechnology Co ltd
Priority date: 2021-05-07
Filing date: 2021-05-07
Publication date: 2022-03-08
Anticipated expiration: 2041-05-07
Also published as: CN113171319A

Abstract

The invention provides a compound plant extract for resisting aging and removing wrinkles in an instant and long-acting manner, and a preparation method and application thereof. The compound plant extract is prepared by combining three plants, namely roselle, monkey bread fruit and pomegranate fruit, the roselle, monkey bread fruit and pomegranate fruit are extracted by adopting a high-temperature and current combined technology under a specific proportion, and finally, multiple filtration is carried out to remove impurities, the preparation process is simple, the cost is low, the obtained compound extract has obvious anti-aging and wrinkle-removing effects and takes effect immediately, wrinkles can be obviously removed within 5min, and the compound extract shows excellent long-acting anti-aging and wrinkle-removing effects after long-term application, can be directly used for skin care without compounding other active substances, can also be prepared into different skin care product formulations, is natural and non-irritant, and provides a new choice for the development of a natural skin care product with the instant and long-acting anti-aging and wrinkle-removing effects.

Description

Instant and long-acting anti-aging wrinkle-removing compound plant extract and preparation method and application thereof

Technical Field

The invention relates to the technical field of skin care, in particular to a compound plant extract for resisting aging and removing wrinkles instantly and in a long-acting manner, and a preparation method and application thereof.

Background

The skin is the largest organ of the human body and is responsible for many functions such as protection, sensation, regulation of body temperature, secretion and excretion, absorption and metabolism, immunity, etc. The skin is located on the body surface and is the tissue which shows the aging of the organism at the earliest, the aging reaction is most obvious and easy to observe, and the anti-aging research on the skin has special significance. Which mainly includes natural aging and photoaging. Natural aging is an endogenous programmed process, and refers to the appearance of wrinkles and the laxity of the skin on the body surface caused by factors such as the change of body resistance factors, such as gravity, secretion and immune function, and heredity. Photoaging refers to extrinsic aging caused by environmental factors such as ultraviolet rays, smoking, and exposure to chemicals, in which repeated exposure of ultraviolet rays in sunlight for a long time is the most important factor affecting skin aging in the environment, and is also called photoaging. The long-term repeated irradiation of sunlight ultraviolet rays is the most important factor influencing skin aging in the environment, and is mainly manifested by rough exposed parts, wrinkle deepening and thickening, abnormal tissue structure, irregular pigmentation, vasodilatation, epidermal dyskeratosis and abnormal proliferation, dermal elastic fibrosis, accumulation of degradation products and the like.

At present, the anti-aging and anti-wrinkle action approaches of cosmetics mainly comprise instant wrinkle removal and long-term anti-aging wrinkle removal, wherein instant wrinkle removal is mainly realized by forming a film through macromolecular saccharides or copolymers and filling wrinkle areas, for example, patent 108670896a provides an instant anti-wrinkle polypeptide eye cream which comprises a VP/VA copolymer, a fine euglena polysaccharide and peptide products for cutting off nerve links, such as acetyl hexapeptide-8, snake venom peptide, conopeptide and the like; the long-acting anti-aging is mainly to resist wrinkles by promoting the regeneration of collagen in the dermis, removing free radicals and other modes, such as retinol and derivatives thereof, VC and derivatives thereof, and some plant flavone polyphenols. If the instant and long-acting anti-aging and wrinkle-removing effects are required to be achieved at the same time, one is to cut off neuropeptide substances; the other is to use the macromolecular substance and a plurality of different active ingredients together by compounding, for example, patent CN112043664A provides a composition for removing wrinkles instantly and resisting aging for a long time, which comprises snake-like venom peptide, bee venom peptide, scorpion venom peptide, conotoxin, jellyfish extract and anti-free radical components, but the screening and compounding difficulty of the product is high, the peptide is mainly a synthesis or fermentation source at present, the cost is high, the product is difficult to benefit the public, and the hidden trouble of synthesis solvent residue or endotoxin residue may exist.

The natural plant-derived components have the advantages of low cost and high safety, but the existing pure plant-derived instant and long-acting anti-aging and anti-wrinkle products are few, and the pure plant-derived anti-aging products are difficult to achieve the obvious instant and long-acting anti-aging effect at the same time, so that the development of a novel pure natural instant and long-acting anti-aging and anti-wrinkle product is urgently needed.

Disclosure of Invention

The invention aims to provide a composite plant extract with instant and long-acting anti-aging and wrinkle-removing effects, and a preparation method and application thereof. The composite plant extract disclosed by the invention is effective immediately after being used, can realize the facial wrinkle improvement rate of more than 15% after being used for 5min, and shows excellent long-acting anti-aging wrinkle-removing effect after being applied for a long time.

The invention aims to provide a preparation method of a compound plant extract for resisting aging and removing wrinkles instantly and in a long-acting manner.

The invention also aims to provide the composite plant extract prepared by the method.

The invention also aims to provide the application of the composite plant extract in preparing the skin care product with instant and long-acting anti-aging and wrinkle-removing functions.

Another object of the present invention is to provide an instant and long-lasting anti-aging and wrinkle-removing skin care product.

Still another object of the present invention is to provide an instant, long-lasting anti-aging wrinkle-removing lotion.

The above purpose of the invention is realized by the following technical scheme:

a preparation method of a compound plant extract for resisting aging and removing wrinkles instantly and in a long-acting manner comprises the following steps:

s1, drying roselle, monkey bread fruit and pomegranate fruit respectively, crushing and sieving to obtain roselle powder, monkey bread fruit powder and pomegranate fruit powder;

s2, mixing the following components in parts by weight of 1-5: 5-10: 3-9: 20-90 mixing roselle powder, monkey bread fruit powder, pomegranate fruit powder and water, extracting at 70-90 ℃ for 30-90 min, introducing 100-200V voltage into the extracting solution, and continuously extracting for 30-90 min to obtain a crude extract;

s3, filtering the crude extract obtained in the step S2 by using a plate-and-frame filter press, eluting the filtrate by adding water through a ceramic membrane, and concentrating the eluent to obtain the composite plant extract.

The invention selects three plants of roselle, monkey bread fruit and pomegranate fruit to jointly prepare the composite plant extract, and the roselle, monkey bread fruit and pomegranate fruit are extracted by adopting a high-temperature and current combined technology under the control of a specific proportion, wherein the high temperature can dissolve out the active ingredients of plant cell walls to the maximum extent, and under the dual action of current and high temperature, the active ingredients in the plants can be more rapidly exuded, so that the active ingredients are partially decomposed, and polysaccharides with different molecular weights and urolithin derived from pomegranate are obtained; finally, impurities in the extract are removed through multiple filtration, the obtained components have good stability, obvious anti-aging and wrinkle-removing effects, can take effect immediately, can remove wrinkles after 5min, has excellent long-acting anti-aging and wrinkle-removing effects after long-term application, can directly protect skin without compounding other active substances, and has the advantages of simple preparation method, low cost and natural nonirritant.

Preferably, the aperture of the filtering membrane of the plate-and-frame filter press in the step S3 is 0.5-2 um.

Preferably, the membrane aperture of the ceramic membrane in the step S3 is 0.1-0.5 um.

Preferably, in the step S3, the water is added to elute the water with 5-10 times of the volume of the filtrate.

Preferably, the concentration in step S3 is carried out at 50-60 ℃ under reduced pressure until the volume before elution is reached, namely the volume of the same filtrate.

Preferably, the drying in the step S1 is drying for 48-96 hours at 40-60 ℃ by blowing until the water content is 0.1-0.5%.

Preferably, the crushing and sieving in the step S1 is to crush the mixture by a crusher and sieve the mixture through a 70-90 mesh sieve.

The compound extract prepared by the method has proper molecular weight distribution of polysaccharide, higher contents of polyphenol, flavone and urolithin, can effectively promote the expression of collagen of human skin fibroblasts, has obvious instant long-acting anti-wrinkle effect, takes effect instantly, can remove wrinkles within 5min, and has excellent long-acting anti-aging and anti-wrinkle effects after long-term application.

Therefore, the composite plant extract prepared by the method is also within the protection scope of the invention.

The compound plant extract prepared by the method contains 1.2 to 1.5 percent of polyphenol, 0.5 to 0.9 percent of flavone, 0.06 to 0.12 percent of urolithin and 0.9 to 1.5 percent of total sugar; wherein, the saccharides with molecular weight less than 10kDa account for 9-12 percent of the total sugar mass, the saccharides with molecular weight less than 10kDa < 100kDa account for 18-22 percent of the total sugar mass, the saccharides with molecular weight less than 100kDa < 3000kDa account for 23-28 percent of the total sugar mass, and the saccharides with molecular weight more than 3000kDa account for 44-47 percent of the total sugar mass. The roselle, monkey bread fruit and pomegranate fruit compound extract meeting the content of the effective components has the effect of resisting aging and removing wrinkles instantly and slowly, so the invention also requests to protect the compound plant extract with the effect of resisting aging and removing wrinkles instantly and slowly, and the compound plant extract is obtained by extracting the roselle, monkey bread fruit and pomegranate fruit with water at high temperature and under voltage, and contains the components with the content.

The invention also claims application of the composite plant extract in preparing a skin care product with instant and long-acting anti-aging and wrinkle-removing functions.

In addition, an instant and long-acting anti-aging and wrinkle-removing skin care product containing the composite plant extract is also within the protection scope of the invention.

Preferably, the instant and long-acting anti-aging and wrinkle-removing skin care product is prepared by adding one or more of auxiliary materials acceptable in daily chemical products, such as solubilizer, preservative, antioxidant, pH regulator, penetration enhancer, liposome, humectant, thickener, chelating agent, skin feel regulator, surfactant, emulsifier, essence and pigment into the composite plant extract to prepare different skin care product dosage forms. For example, cream, emulsion, solution, film, aerosol or spray form, and the skin care product may be lotion, essence, mask, lotion, cream, eye cream, etc.

The invention provides an instant and long-acting anti-aging wrinkle-removing toning lotion, which comprises the following components in parts by weight:

3-8 parts of glycerol, 1-10 parts of butanediol, 0.05-2.0 parts of xanthan gum, 1-10 parts of the composite plant extract, 0.1-1.0 part of p-hydroxyacetophenone, 0.1-1.0 part of 1, 2-hexanediol and water for supplementing 100 parts.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a preparation method of a natural plant composite extract with instant and long-acting anti-aging and wrinkle-removing effects, by controlling a specific preparation process, the obtained compound extract has proper polysaccharide molecular weight distribution and higher contents of polyphenol, flavone and urolithin, can effectively promote the expression of collagen of human skin fibroblasts, has remarkable anti-aging and wrinkle-removing effects, the skin care product takes effect immediately, can realize that the improvement rate of facial wrinkles reaches more than 15 percent after being used for 5min, also has excellent long-acting anti-aging and wrinkle-removing effects after being used for a long time, can directly care skin without compounding other active functional components, can also be added with auxiliary materials acceptable in skin care products to be prepared into different skin care product formulations, has good anti-aging and wrinkle-removing effects, and the preparation process of the composite extract is simple, and a new choice is provided for the development of the natural skin care product for resisting aging and removing wrinkles in an instant and long-acting manner.

Drawings

FIG. 1 shows the molecular weight distribution of polysaccharides in a complex extract;

FIG. 2 shows the effect of complex extracts on the promotion of collagen expression in human skin fibroblasts;

FIG. 3 shows the improvement of facial wrinkles before and after the use of the complex extract;

FIG. 4 shows the immediate and long-acting anti-wrinkle effects of astringent 1-3.

Detailed Description

The invention will be further described with reference to the drawings and the detailed description, which are not intended to limit the invention in any way. The starting reagents employed in the examples of the present invention are, unless otherwise specified, those that are conventionally purchased.

Example 1 preparation of Complex plant extract 1

1. Taking fresh roselle, monkey bread fruits and pomegranate fruits, and drying the fresh roselle, monkey bread fruits and pomegranate fruits by blowing at 50 ℃ for 72 hours to respectively obtain dried roselle, monkey bread fruits and pomegranate fruits with the water content of 0.3%;

2. respectively crushing the obtained dried roselle, monkey bread fruits and pomegranate fruits in a crusher, and sieving the crushed products with a 80-mesh sieve to obtain dark red roselle powder, powder-like white monkey bread fruit powder and brown pomegranate powder;

3. mixing 2 parts of roselle powder, 8 parts of Adiantum montanum powder, 5 parts of pomegranate fruit powder and 75 parts of distilled water in proportion, extracting at 80 ℃ for 55min, introducing 175V voltage into the extracting solution, and continuously extracting for 45min to obtain a crude extract;

4. filtering the crude extract with plate-and-frame filter press, wherein the aperture of the used filtering membrane is 1.2um, eluting the filtrate with 0.2um ceramic membrane and 8 times of volume of distilled water, and concentrating the eluate at 50 deg.C under reduced pressure until the volume reaches the volume of the filtrate before elution to obtain the composite plant extract 1.

Example 2 preparation of Complex plant extract 2

1. Taking fresh roselle, monkey bread fruits and pomegranate fruits, and drying the fresh roselle, monkey bread fruits and pomegranate fruits by blowing at 55 ℃ for 68 hours to respectively obtain dried roselle, monkey bread fruits and pomegranate fruits with the water content of 0.25%;

3. mixing 3 parts of roselle powder, 7 parts of Adiantum montanum powder, 6 parts of pomegranate fruit powder and 80 parts of distilled water in proportion, extracting at 80 ℃ for 60min, introducing 170V voltage into the extracting solution, and continuously extracting for 40min to obtain a crude extract;

4. filtering the crude extract with plate-and-frame filter press, wherein the aperture of the used filtering membrane is 1.5um, eluting the filtrate with 0.3um ceramic membrane and 7 times of distilled water, and concentrating the eluate at 60 deg.C under reduced pressure until the volume reaches the volume of the filtrate before elution to obtain the compound plant extract 2.

Example 3 preparation of Complex plant extract 3

1. Taking fresh roselle, monkey bread fruits and pomegranate fruits, and drying the fresh roselle, monkey bread fruits and pomegranate fruits by blowing at 40 ℃ for 96 hours to respectively obtain dried roselle, monkey bread fruits and pomegranate fruits with the water content of 0.1%;

3. mixing 1 part of roselle powder, 10 parts of Adiantum montanum powder, 3 parts of pomegranate fruit powder and 90 parts of distilled water in proportion, extracting at 90 ℃ for 30min, introducing 100V voltage into the extracting solution, and continuously extracting for 30min to obtain a crude extract;

4. filtering the crude extract with plate-and-frame filter press, eluting the filtrate with 0.5um ceramic membrane and 10 times of distilled water, and concentrating the eluate at 50 deg.C under reduced pressure until the volume reaches the volume of the filtrate before elution to obtain composite plant extract 3.

Example 4 preparation of Complex plant extract 4

1. Taking fresh roselle, monkey bread fruits and pomegranate fruits, and drying the fresh roselle, monkey bread fruits and pomegranate fruits by blowing at the temperature of 60 ℃ for 48 hours to respectively obtain dried roselle, monkey bread fruits and pomegranate fruits with the water content of 0.5%;

3. mixing 5 parts of roselle powder, 5 parts of Adiantum commune powder, 9 parts of pomegranate fruit powder and 20 parts of distilled water in proportion, extracting at 70 ℃ for 90min, introducing 200V voltage into the extracting solution, and continuously extracting for 30min to obtain a crude extract;

4. filtering the crude extract with plate-and-frame filter press, wherein the aperture of the used filtering membrane is 0.5um, eluting the filtrate with 0.1um ceramic membrane and 5 times of distilled water, and concentrating the eluate at 50 deg.C under reduced pressure until the volume reaches the volume of the filtrate before elution to obtain compound plant extract 4.

Example 5 preparation of Complex plant extract 5

3. mixing 5 parts of roselle powder, 5 parts of Adiantum commune powder, 3 parts of pomegranate fruit powder and 90 parts of distilled water in proportion, extracting at 90 ℃ for 30min, introducing 100V voltage into the extracting solution, and continuously extracting for 30min to obtain a crude extract;

4. filtering the crude extract with plate-and-frame filter press, wherein the aperture of the used filtering membrane is 2um, eluting the filtrate with 0.5um ceramic membrane and 10 times of distilled water, and concentrating the eluate at 50 deg.C under reduced pressure until the volume reaches the volume of the filtrate before elution to obtain the composite plant extract 5.

Example 6 preparation of composite plant extract 6

3. mixing 1 part of roselle powder, 10 parts of Adiantum montanum powder, 3 parts of pomegranate fruit powder and 20 parts of distilled water in proportion, extracting at 70 ℃ for 90min, introducing 200V voltage into the extracting solution, and continuously extracting for 30min to obtain a crude extract;

4. filtering the crude extract with plate-and-frame filter press, wherein the aperture of the used filtering membrane is 0.5um, eluting the filtrate with 0.1um ceramic membrane and 5 times of distilled water, and concentrating the eluate at 50 deg.C under reduced pressure until the volume reaches the volume of the filtrate before elution to obtain the compound plant extract 6.

Comparative example 1

The preparation of the composite plant extract of example 1 was identical except that in step 3, 0.5 parts of roselle powder, 4.5 parts of Adansonia dubia powder, and 10 parts of pomegranate fruit powder were used.

Comparative example 2

The preparation of the composite plant extract of example 1 was identical except that, in step 3, 6 parts of roselle powder, 3 parts of monkey bushy powder and 6 parts of pomegranate fruit powder were used.

Comparative example 3

The preparation of the composite plant extract of example 1 was identical except that in step 3, 0 part of roselle powder, 10 parts of monkeytree powder and 5 parts of pomegranate fruit powder were used.

Comparative example 4

The preparation of the composite plant extract of example 1 was identical except that in step 3, roselle powder was 10 parts, monkey bushy powder was 0 part, and pomegranate fruit powder was 5 parts.

Comparative example 5

The preparation of the composite plant extract of example 1 was identical except that in step 3, roselle powder was 7 parts, monkey bushy powder was 8 parts, and pomegranate fruit powder was 0 part.

Comparative example 6

The preparation of the composite plant extract of example 1 was identical, except that, in the 3 rd step, the extraction temperature was 60 ℃.

Comparative example 7

The preparation of the composite plant extract of example 1 was identical, except that, in the 3 rd step, the extraction temperature was 95 ℃.

Comparative example 8

The preparation of the composite plant extract of example 1 was identical except that in step 3, the voltage was 85V.

Comparative example 9

The preparation of the composite plant extract of example 1 was identical except that in step 3, the voltage was 220V.

Comparative example 10

The preparation of the composite plant extract of example 1 was carried out with the difference that in step 3, the electric extraction was not carried out.

Comparative example 11

The preparation of the composite plant extract of example 1 was identical except that in step 4, a plate and frame filter press was used with a filter membrane having a pore size of 0.4um, followed by elution through a 0.08um ceramic membrane.

Comparative example 12

The preparation of the composite plant extract of example 1 was identical except that in step 4, a plate and frame filter press was used with a filter membrane having a pore size of 0.25um, followed by elution through a 0.8um ceramic membrane.

Comparative example 13

The preparation of the composite plant extract of example 1 was identical except that in step 4, elution was not carried out through a ceramic membrane.

Experimental example 1 measurement of content of plant extract component

The content of the components was measured with respect to the complex plant extracts prepared in examples 1 to 6 and comparative examples 1 to 13.

1. Experimental methods

Testing the total polyphenol content by Folin-Ciocalteu colorimetric method;

the total flavone content adopts NaNO2-Al (NO)₃)₃-NaOH test method;

the total sugar content is measured by adopting a phenol-sulfuric acid method;

the content of urolithin A is detected by HPLC method.

2. Test results

TABLE 1

	Total Polyphenol content (%)	Total flavone content (%)	Total sugar content (%)	Urolithin content (%)	Appearance of the composition
						Example 1	1.45	0.82	1.43	0.11	Light yellow clear slightly viscous liquid
Example 2	1.4	0.74	1.12	0.08	Light yellow clear slightly viscous liquid
						Example 3	1.3	0.68	1.11	0.07	Yellow viscous liquid
Example 4	1.25	0.55	1.01	0.06	Light yellow clear slightly viscous liquid
						Example 5	1.3	0.6	1.1	0.07	Yellow clear viscous liquid
Example 6	1.2	0.54	0.98	0.06	Light yellow clear slightly viscous liquid
						Comparative example 1	0.74	0.52	0.68	0.048	Light yellow clear liquid
Comparative example 2	0.75	0.42	0.45	0.05	Light yellow clear liquid
						Comparative example 3	0.02	0.01	1.35	0.085	Colorless, clear and slightly viscous liquid
Comparative example 4	1.24	0.74	0.01	0.087	Light yellow clear liquid
						Comparative example 5	1.3	0.68	1.32	0	Light yellow clear slightly viscous liquid
Comparative example 6	0.74	0.62	0.71	0.052	Light yellow clear liquid
						Comparative example 7	0.75	0.71	0.85	0.15	Light yellow clear liquid
Comparative example 8	0.071	0.65	0.52	0.038	Light yellow clear liquid
						Comparative example 9	0.11	0.32	1.61	0.04	Light yellow clear slightly viscous liquid
Comparative example 10	0.68	0.48	0.68	0.059	Colorless clear liquid
						Comparative example 11	1.51	0.92	1.56	0.11	Yellow brown clear viscous liquid
Comparative example 12	1.29	0.69	1.41	0	Light yellow clear slightly viscous liquid
						Comparative example 13	1.75	1.21	2.3	0.14	Brown foggy viscous liquid

As can be seen from the results in Table 1, the content of the four active substances in the composite plant extracts obtained in comparative examples 1 to 5 is lower than the level of the composite plant extracts obtained in examples 1 to 6, which indicates that the invention can simultaneously achieve higher contents of polyphenol, flavone, total sugar and urolithin by controlling the dosage ratio of the three specific plants, and the content of the four active substances in the composite extracts obtained by reducing the dosage of any one plant material or lacking any one plant material can not simultaneously reach the level of the composite plant extracts obtained in examples 1 to 6.

In addition, the preparation processes of comparative examples 6-9 and 11-12 are different from the preparation process of the present invention, the content of four active substances in the obtained plant extract can not reach the content range of 4 active substances in the extracts of examples 1-6 at the same time, and although the content of 4 active substances in the extract obtained in comparative example 11 is higher, the color of the extract is dark, which is not favorable for the application. In contrast, in comparative examples 10 and 13, the effect of the present invention was not obtained in the content of active substances or in the appearance of the extract, as shown in example 1, without performing the electrical extraction operation or the ceramic membrane elution operation.

Experimental example 2 measurement of polysaccharide molecular weight distribution of plant extract and experiment of facial filming of volunteers

1. Experimental methods

Because the extract obtained by the invention mainly contains polysaccharide which has the effect of instant film forming and instant wrinkle resistance, the polysaccharide content is low, the film forming effect is poor, only a sample with the polysaccharide content of 1% is tested for the molecular weight distribution, and the molecular weight distribution of the polysaccharide in the compound extract obtained by the High Performance Gel Permeation Chromatography (HPGPC) is detected in examples 1-5 and comparative examples 3, 5, 9, 11, 12 and 13.

Volunteer facial filming experiment: 11 subjects were selected, the complex extracts obtained in examples 1 to 5 and comparative examples 3, 5, 9, 11, 12 and 13 were mixed with ultrapure water to prepare 5% aqueous solutions, 0.5mL of the aqueous solutions was applied to the eye periphery, and after 5min, the film formation was observed and the transdermal water loss and water content of the skin were measured.

2. Test results

TABLE 2 results of periocular smear of plant extracts for film formation, transdermal water loss and epidermal water content

	Film forming effect after 5min	Rate of water loss through skin	Moisture content of epidermis
				Example 1	Uniform, flat and without rubbing mud	15%	20%
Example 2	Uniform, flat and without rubbing mud	16%	18%
				Example 3	Uniform, flat and without rubbing mud	17%	19%
Example 4	Uniform, flat and without rubbing mud	18%	18%
				Example 5	Uniform, flat and without rubbing mud	16%	17%
Comparative example 3	Even, smooth and slightly rub mud	25%	8%
				Comparative example 5	Even, smooth and slightly rub mud	30%	7%
Comparative example 9	Even, smooth and slightly rub mud	28%	6%
				Comparative example 11	Uniform, smooth and rub mud	30%	7%
Comparative example 12	Uniform, smooth and rub mud	25%	5%
				Comparative example 13	Even, flat and seriously rubbed mud	24%	6%

FIG. 1 shows the molecular weight distribution of polysaccharides in the complex extract, and in examples 1 to 6, saccharides having a molecular weight of < 10kDa account for 9 to 12% of the total mass of saccharides, saccharides having a molecular weight of < 10kDa < 100kDa account for 18 to 22% of the total mass of saccharides, saccharides having a molecular weight of < 100kDa account for 23 to 28% of the total mass of saccharides, and saccharides having a molecular weight of > 3000kDa account for 44 to 47% of the total mass of saccharides. Table 2 shows the results of the plant extract including the periocular smear film formation, the transdermal water loss rate and the epidermal water content, and it can be seen from the molecular weight distribution of polysaccharides, the periocular smear film formation, the transdermal water loss of skin and the stratum corneum water content in fig. 1 and 2 that the plant extracts prepared in examples 1 to 5 have the distribution of large molecular polysaccharides and small molecular polysaccharides favorable for film formation and low transdermal water loss and high stratum corneum water content, while the composite extracts obtained in comparative examples 3, 5, 9, 11, 12 and 13 have the tendency of the polysaccharides to be distributed mostly in the large molecular weight region, have good film formation property, but are easily slubbed, and have short water absorption duration due to their very small molecular polysaccharides.

Experimental example 3 in vitro promotion of collagen type 1 production

1. Experimental methods

2 ml of human skin fibroblasts cultured to log phase at 3X 10⁵Each/ml concentration was plated on a plate with a cover glass, the cover glass was treated with polylysine before the incubation, and after 24 hours of incubation with DMEM medium containing 10% FBS, 3 replicates of examples 1-6 and comparative examples 1-13 were added at concentrations of 1, 10, 50, 100. mu.g/ml, respectively, and an equal volume of serum was added to the control group, and after 7 days of incubation, collagen production was detected.

2. Test results

Fig. 2 shows the effect of the composite extract on the promotion of collagen expression in human skin fibroblasts, and it can be seen from fig. 2 that the promotion effects of examples 1-6 on collagen expression in type 1 are significantly stronger than those of the comparative examples, and the increase rate of the extract prepared by the invention on collagen in type 1 is about 56% higher than that of the comparative examples. The compound extract prepared by the method has better in-vitro anti-aging effect.

Experimental example 4 immediate and long-acting anti-wrinkle efficacy test

1. Experimental methods

1) The 19 composite extracts obtained in examples 1-6 and comparative examples 1-13 were mixed with ultrapure water to prepare 5% skin lotion, and the 19 skin lotions were subjected to anti-wrinkle tests, respectively, to obtain 190 skin lotions for each group of 10 skin lotions, wherein each group of skin lotions was prepared by washing the face of the test person, photographing the face with VISA-CR in a constant temperature and humidity environment (temperature 21-23 deg.C, humidity 55-65%) for 30min, spraying 3 pumps of anti-aging lotion 25CM away from the face, patting gently to absorb the lotion, and photographing the face with VISA-CR after standing for 5 min. And then calculating the improvement rate of the facial wrinkles before and after use by instrument software to reflect the instant improvement condition of the facial wrinkles by each skin lotion, namely the instant anti-wrinkle effect.

2) And (5) long-acting anti-wrinkle test. The test person adhered to the use of the complex extract for 56 days, and photographs were taken after washing the face on days 28 and 56.

2. Test results

Fig. 3 shows the facial wrinkle improvement before and after the use of the composite extract, and it can be seen from the results of fig. 3 that the anti-wrinkle effect and the long-lasting anti-wrinkle effect of the skin lotion prepared by using the extracts obtained in examples 1 to 6 are significantly better than those of comparative examples 1 to 13, the instant wrinkle removal rate of the extracts of examples 1 to 6 at 5min is higher than 15%, the instant anti-wrinkle effect of the extracts of examples is higher than that of comparative example 162 on average, and the long-lasting anti-wrinkle effect of the extracts of examples is higher than that of comparative example 50 on average. The extract prepared according to the invention is shown to have good immediate anti-wrinkle and long-acting wrinkle-removing effects.

Example 7 toner 1

The embodiment provides a toning lotion which comprises the following components in parts by weight:

5 parts of glycerol, 3 parts of butanediol, 0.1 part of xanthan gum, 5 parts of the composite plant extract prepared in example 1, 0.5 part of p-hydroxyacetophenone, 0.5 part of 1, 2-hexanediol and the balance of water to 100 parts.

Example 8 toner 2

8 parts of glycerol, 1 part of butanediol, 2.0 parts of xanthan gum, 1 part of compound plant extract prepared in example 1, 1.0 part of p-hydroxyacetophenone, 0.1 part of 1, 2-hexanediol and the balance of water to 100 parts

Example 9 toner 3

8 parts of glycerol, 1 part of butanediol, 0.05 part of xanthan gum, 10 parts of the composite plant extract prepared in example 1, 0.1 part of p-hydroxyacetophenone, 1.0 part of 1, 2-hexanediol and the balance of water to 100 parts.

Experimental example 5 measurement of cosmetic Water Properties

The immediate and long-acting anti-wrinkle effects of lotions 1 to 3 were tested in the same manner as in experimental example 4, and the test results are shown in fig. 4. As can be seen from the results of fig. 4, the lotion prepared by using the extract of the present invention also exhibited excellent immediate, long-lasting anti-wrinkle effects, with an average improvement rate of immediate wrinkles of up to 20% and an average improvement rate of long-lasting wrinkles of up to 22%.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. An instant and long-acting anti-aging and wrinkle-removing compound plant extract is characterized in that roselle, monkey bread fruits and pomegranate fruits are extracted by water under high temperature and voltage, wherein the compound plant extract contains 1.2-1.5% of polyphenol, 0.5-0.9% of flavone, 0.06-0.12% of urolithin and 0.9-1.5% of total sugar;

wherein, in the total sugar, the saccharides with molecular weight less than 10kDa account for 9-12 percent of the total sugar mass, the saccharides with molecular weight less than 10kDa < 100kDa account for 18-22 percent of the total sugar mass, the saccharides with molecular weight less than 100kDa < 3000kDa account for 23-28 percent of the total sugar mass, and the saccharides with molecular weight more than 3000kDa account for 44-47 percent of the total sugar mass;

the preparation method of the instant and long-acting anti-aging and wrinkle-removing compound plant extract comprises the following steps:

s2, mixing the components in a weight ratio of 1-5: 5-10: 3-9: 20-90 mixing roselle powder, monkey bread fruit powder, pomegranate fruit powder and water, extracting at 70-90 ℃ for 30-90 min, introducing 100-200V voltage into the extracting solution, and continuously extracting for 30-90 min to obtain a crude extract;

s3, filtering the crude extract obtained in the step S2 by using a plate-and-frame filter press, eluting the filtrate by adding water through a ceramic membrane, and concentrating the eluent to obtain the composite plant extract;

the aperture of the filtering membrane of the plate-and-frame filter press in the step S3 is 0.5-2 um; and S3, the membrane aperture of the ceramic membrane is 0.1-0.5 um.

2. The composite plant extract as claimed in claim 1, wherein the step S3 of the preparation method comprises adding water to elute with 5-10 times of the volume of the filtrate.

3. The composite plant extract as claimed in claim 1, wherein the concentration in step S3 is carried out at 50-60 deg.C under reduced pressure to a volume before elution.

4. The composite plant extract as claimed in claim 1, wherein the drying in step S1 is performed by air-blast drying at 40-60 deg.C for 48-96 h until the water content is 0.1-0.5%; and step S1, the crushing is carried out by adopting a crusher and sieving by a sieve of 70-90 meshes.

5. Use of the composite plant extract of any one of claims 1 to 4 in the preparation of a skin care product with immediate and long-lasting anti-aging and wrinkle-removing effects.

6. An instant and long-acting anti-aging and wrinkle-removing skin care product, which is characterized by comprising the compound plant extract as claimed in any one of claims 1 to 4.

7. The skin care product of claim 6, wherein the skin care product comprises a lotion, a serum, a mask, an emulsion, a body lotion, a cream, and an eye cream.

8. An instant and long-acting anti-aging wrinkle-removing toning lotion is characterized by comprising the following components in parts by weight:

3-8 parts of glycerin, 1-10 parts of butanediol, 0.05-2.0 parts of xanthan gum, 1-10 parts of the composite plant extract of any one of claims 1-4, 0.1-1.0 part of p-hydroxyacetophenone, 0.1-1.0 part of 1, 2-hexanediol and the balance of water to 100 parts.