CN112972328A - Phellodendron and liquorice acne-removing plant composition and application thereof - Google Patents
Phellodendron and liquorice acne-removing plant composition and application thereof Download PDFInfo
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- CN112972328A CN112972328A CN202110213010.3A CN202110213010A CN112972328A CN 112972328 A CN112972328 A CN 112972328A CN 202110213010 A CN202110213010 A CN 202110213010A CN 112972328 A CN112972328 A CN 112972328A
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- phellodendron
- acne
- liquorice
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Abstract
The invention discloses a phellodendron and liquorice acne-removing plant composition which comprises the following components in parts by weight: cortex Phellodendri extract10～90Preparing; glycyrrhiza extract10～90Parts by weight of100And (4) portions are obtained. According to the phellodendron and liquorice acne-removing plant composition, phellodendron and liquorice are directly compounded to form the plant composition, so that the proliferation of pathogenic bacteria related to acne can be effectively inhibited, and the treatment, prevention and treatment of acne are achieved. The invention also discloses application of the phellodendron and liquorice acne-removing plant composition.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to a phellodendron and liquorice acne-removing plant composition and application thereof.
Background
Acne is commonly called as whelk, is good for young men and women, and is a common chronic inflammatory dermatosis. The factors causing acne are various, mainly due to excessive sebum secretion, follicular sebaceous gland blockage, bacterial infection and the like. Among them, bacterial infection is the most important factor for inducing the occurrence of acne. Normally, many microorganisms including propionibacterium acnes are present in the surface layer of the skin and in the hair follicles of the sebaceous glands in a large amount, and after the microorganisms proliferate in a large amount, lipases are produced to decompose sebum to form free fatty acids, which in turn chemotactic inflammatory cells and mediators, and finally induce and aggravate inflammatory reactions.
Modern medicine mainly relies on some medicines such as antibiotics and hormones for treating acne, and although the medicines have effects, the medicines have some risks of drug resistance and adverse reactions. For example:
the Chinese Magnetorubin dermatosis journal 2019,35(9):515-519 published Zhang Liqi et al Meta analysis of antibiotic resistance of Propionibacterium acnes in the skin lesions of acne patients in China, and researches show that the Propionibacterium acnes separated from the skin lesions of acne patients in China have high drug resistance to macrolides and metronidazole and are sensitive to tetracyclines and quinolones antibiotics, wherein the sensitivity to minocycline is highest.
Northern pharmacy 2020,17(8):136-138 published 'observation of curative effect and safety evaluation of minocycline hydrochloride combined azelaic acid gel for treating acne rosacea' of xi shun zhen et al finds that minocycline hydrochloride combined azelaic acid gel has significant curative effect in treating acne rosacea, but still has 5.77% adverse drug reactions.
Many natural plants have good acne inhibition effect, are safer than chemical drugs, are milder and more friendly to skin, can well exert the effect, and can improve drug resistance and adverse reactions. For example:
the Heilongjiang medicine, 2019,43(8): 937-containing 937 published on gorgeous 'clinical efficacy observation of treating acne rosacea with compound cortex phellodendri liquid liniment', and researches show that the compound cortex phellodendri liquid liniment is safe and effective for treating the acne rosacea, and no serious adverse reaction occurs in the treatment process.
Asia Pacific traditional medicine, 2017,13(1):151-152 published the clinical efficacy observation of the formula of the land formula for treating mild and moderate acne by combining the licorzinc granules with the traditional Chinese medicine acne formula, and researches show that the treatment by combining the licorzinc granules with the traditional Chinese medicine acne formula can obtain obvious efficacy aiming at mild and moderate acne, and is worthy of clinical popularization.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides the phellodendron and liquorice acne-removing plant composition which can effectively inhibit the proliferation of pathogenic bacteria related to acne so as to treat and prevent the acne, has the excellent characteristics of high safety, obvious inhibition on the acne and the like, and has good market value and application prospect.
The invention also aims to provide application of the phellodendron and liquorice acne-removing plant composition.
One technical scheme for achieving the purpose is as follows: the phellodendron and liquorice acne-removing plant composition comprises the following components in parts by weight:
10-90 parts of a phellodendron extract;
10-90 parts of a licorice extract;
the sum of the weight parts of the phellodendron extract and the liquorice extract is 100 parts.
The phellodendron and liquorice acne-removing plant composition comprises the following preparation process of phellodendron extract: weighing dry medicinal materials of golden cypress, crushing, adding the medicinal materials into a pure water solution, performing reflux extraction for 1-4 times, mixing obtained extracting solutions, and performing reduced pressure concentration and water bath evaporation to dryness in sequence to obtain a golden cypress extract;
the ratio of the materials to the liquid of the dry medicinal materials of the golden cypress to the pure water is 1: 6-1: 20kg/L during each extraction.
The phellodendron and liquorice acne-removing plant composition is characterized in that the content of berberine in the phellodendron extract is not less than 9%, and the content of phellodendrine is not less than 3%.
The phellodendron and liquorice acne-removing plant composition comprises the following preparation process of liquorice extract: weighing dry licorice, crushing, adding the crushed dry licorice into an alcohol/water mixed solution, performing reflux extraction for 1-4 times, mixing obtained extracting solutions, and performing reduced pressure concentration and water bath evaporation to dryness in sequence to obtain a licorice extract;
in each extraction, the material-liquid ratio of the dry licorice and the alcohol/water mixed solution is 1: 6-1: 20 kg/L;
the alcohol in the alcohol/water mixed solution is one or more of methanol, ethanol, pentanediol and butanediol;
the volume fraction of the alcohol in the alcohol/water mixed solution is 30-95%.
The phellodendron and liquorice acne-removing plant composition is characterized in that glycyrrhizic acid in the liquorice extract is not less than 5% by weight.
The invention also provides an application of the phellodendron and liquorice acne-removing plant composition, wherein the phellodendron and liquorice acne-removing plant composition is added into cosmetics, and the mass of the phellodendron and liquorice acne-removing plant composition is 0.3-0.5% of the total mass of the cosmetics.
The phellodendron and liquorice acne-removing plant composition is applied, wherein the cosmetics are moisturizing cream or moisturizing lotion.
The phellodendron and liquorice acne-removing plant composition is applied, wherein the moisturizing cream comprises the following components in parts by weight: 0.01-0.05 part of acrylic acid (ester), 0.8-1.2 parts of polydimethylsiloxane, 0.01-0.03 part of xanthan gum and 0 part of sodium hyaluronate.01～0.03 portions and thoroughlyHyaluronic acid 0.01～0.03 parts, 1-3 parts of tridecyl alcohol trimellitate, 1-3 parts of shea butter, 0.05-0.15 part of prinsepia utilis royle oil, 0.5-0.15 part of glycerol stearate, 0.04-0.2 part of purslane extract, 0.05-0.15 part of aminomethyl propanol, 0.3-0.5 part of phellodendron and liquorice acne-removing plant composition and the balance of water;
the sum of the weight parts of the components of the moisturizing cream is 100 parts.
The application of the phellodendron and liquorice acne-removing plant composition is that the skin lotion comprises the following components in parts by weight: 1-5 parts of glycerol, 261-5 parts of glyceryl polyether, 0.03-0.08 part of hydroxyethyl cellulose, 0.01-0.05 part of sodium hyaluronate, 0.04-0.2 part of beta-glucan, 0.04-0.2 part of purslane extract, 0.3-0.5 part of phellodendron and liquorice acne-removing plant composition and the balance of water;
the sum of the parts by weight of the components of the emollient water is 100 parts.
The phellodendron and liquorice acne-removing plant composition disclosed by the invention is intended to effectively reduce the dosage proportion of antibiotics and hormones in cosmetics through the acne-removing efficacy of phellodendron and liquorice, so that potential safety risks are avoided as far as possible. In addition, the final phellodendron and liquorice acne-removing plant composition has good acne-removing effect and potential application value by compounding and controlling the content of active ingredients in the phellodendron extract and the liquorice extract.
Compared with research reports of other researchers, the phellodendron-liquorice acne-removing plant composition and the application thereof disclosed by the invention have the following advantages:
(1) the phellodendron and the liquorice are directly compounded and combined into the plant composition for the first time, and the plant composition is tested for the acne related pathogenic bacteria for the first time: the inhibition effect of propionibacterium acnes, staphylococcus epidermidis and staphylococcus aureus shows that the plant composition can well inhibit pathogenic bacteria related to acne;
(2) in order to ensure that the phellodendron and liquorice acne-removing plant composition has good effect of inhibiting acne-related pathogenic bacteria, in the phellodendron and liquorice acne-removing plant composition, the active ingredients of a phellodendron extract are as follows: the berberine content is not less than 9%, and the phellodendrine content is not less than 3%; the effective components of the licorice extract are as follows: the glycyrrhizic acid content is not lower than 5%;
(3) the phellodendron-liquorice acne-removing plant composition is added into an actual formula, and the antibacterial performance is determined according to GB15979-2002 appendix C4' test method for antibacterial performance of dissoluble antibacterial (bacteriostatic) products in sanitation Standard of Disposable sanitary products, and is obvious and effective;
(4) in order to ensure that the phellodendron and liquorice acne-removing plant composition is added into an actual formula to be effective obviously, the mass of the plant composition added into the cosmetics is 0.3-0.5% of the total mass of the cosmetics;
(5) in addition, the phellodendron bark has the effects of clearing heat and drying dampness, purging fire and removing steam, detoxifying and treating sore, and the liquorice has the effects of clearing heat and detoxifying and preventing and treating tumor, and has a very good potential application value.
Drawings
FIG. 1 is an HPLC chart showing the calibration of berberine and phellodendrine contents in the phellodendron extract;
FIG. 2 is an HPLC chart showing the calibration of glycyrrhizic acid content in the licorice extract.
Detailed Description
In order that those skilled in the art will better understand the technical solution of the present invention, the following detailed description is given with reference to the accompanying drawings:
referring to fig. 1, the method for calibrating the content of berberine and phellodendrine in the phellodendron extract is as follows:
HPLC conditions, liquid phase column: agilent Zorbax C18(4.6 x 250mm, 5 μm), wavelength: 284nm, column temperature: 35 ℃, mobile phase: 0.1% phosphoric acid (mobile phase a), acetonitrile (mobile phase B), gradient elution procedure: 0-15min, mobile phase A: 95% → 65%; 15-20min, mobile phase A: 65% → 95%; 20-25min, mobile phase A: 95 percent.
According to the HPLC conditions, taking the berberine hydrochloride concentration (C) as a horizontal coordinate and the peak area (S) as a vertical coordinate to obtain a berberine hydrochloride linear equation: 26702C +31.758, R20.9999, in the concentration range(0.0031～0.1550mg·mL-1) The interior of the filter has good linear relation; taking the concentration (C) of the phellodendrine as the abscissa and the peak area (S) as the ordinate, obtaining a phellodendrine linear equation: 11963C +6.0293, R20.9999, in the concentration range (0.0014-0.0680 mg/mL)-1) The interior of the filter has good linear relation; measuring the content of berberine and phellodendrine in cortex Phellodendri extract, and determining the concentration in the above range for three times and substituting into corresponding berberine hydrochloride and phellodendrine linear equation to obtain the final product. Repeated experiments show that the content of berberine in the phellodendron extract is not less than 9 percent, and the content of phellodendrine in the phellodendron extract is not less than 3 percent.
Referring to fig. 2, the method for calibrating the glycyrrhizic acid content in the licorice extract is as follows:
HPLC conditions, liquid phase column: agilent Zorbax C18(4.6 x 250mm, 5 μm), wavelength: 237nm, column temperature: 35 ℃, mobile phase: 0.05% phosphoric acid (mobile phase a), acetonitrile (mobile phase B), gradient elution procedure: 0-30min, mobile phase A: 80% → 60%; 30-40min, mobile phase A: 60% → 80%; 40-45min, mobile phase A: 80 percent.
According to the related regulation of the 2020 edition pharmacopeia on licorice, namely, the weight of glycyrrhizic acid is equal to the weight of ammonium glycyrrhizinate/1.0207, and the content of glycyrrhizic acid in the final licorice extract is obtained through conversion. According to the HPLC conditions, taking the concentration (C) of the ammonium glycyrrhetate as a horizontal coordinate and the peak area (S) as a vertical coordinate to obtain an ammonium glycyrrhetate linear equation: 3477.9C-0.4547, R20.9993, in the concentration range (0.0082-0.2608 mg/mL)-1) The interior of the filter has good linear relation; and (3) measuring the glycyrrhizic acid content in the licorice extract, ensuring that the converted concentration is measured in parallel for three times in the range and is substituted into a corresponding ammonium glycyrrhizinate linear equation to obtain the ammonium glycyrrhizinate content, and repeating the experiment and converting to obtain the licorice extract in which the glycyrrhizic acid content is not lower than 5%.
Bacteriostatic MIC test
In order to reasonably compound the phellodendron extract and the licorice extract subsequently, the phellodendron extract, the licorice extract and main functional monomers thereof need to be prepared by the following steps: berberine, phellodendrine and glycyrrhizic acid are used for determining the bacteriostatic MIC, and the specific experimental method is as follows:
selecting resuscitation and acne related pathogenic bacteria: propionibacterium acnes (ATCC 11827), Staphylococcus aureus (ATCC 6538), and Staphylococcus epidermidis (ATCC 11228), and bacterial suspensions (OD 600: about 0.25) with a specific concentration were prepared by counting, diluting, and the prepared bacterial suspensions were stored at 4 ℃ for use.
And (3) determining the bacteriostatic MIC of the Propionibacterium acnes: under anaerobic condition, preparing cortex Phellodendri extract into cortex Phellodendri extractive solution with specific concentration by dilution method, adding 3mL cortex Phellodendri extractive solution and 3mL brain heart infusion culture medium (BHI) into each test tube, and adding 0.3mL bacterial suspension to ensure that the concentration of Propionibacterium acnes in each tube is 1-6 × 108And (3) setting a positive control group and a negative control group for CFU/mL, culturing for 96h at 37 ℃, and obtaining the bacteriostatic MIC of the phellodendron extract by comparing turbidity and taking the lowest concentration of the phellodendron extract as the clarification condition by adding the phellodendron extract. The method is also suitable for replacing cortex Phellodendri extract with Glycyrrhrizae radix extract, berberine, phellodendrine and glycyrrhizic acid.
And (3) determining the bacteriostatic MIC of staphylococcus aureus and staphylococcus epidermidis: preparing cortex Phellodendri extract into cortex Phellodendri extractive solution with specific concentration by dilution method at room temperature, adding 3mL cortex Phellodendri extractive solution and 3mL Trypticase Soy Broth (TSB) into each test tube, and adding 0.3mL bacterial suspension to ensure that the concentration of Staphylococcus aureus and Staphylococcus epidermidis in each tube is 1-6 × 108And (3) setting a positive control group and a negative control group for CFU/mL, culturing for 24h at the temperature of 30 ℃, and obtaining the bacteriostatic MIC of the phellodendron extract by comparing turbidity and taking the lowest concentration of the phellodendron extract as the clarification condition by adding the phellodendron extract. The method is also suitable for replacing cortex Phellodendri extract with Glycyrrhrizae radix extract, berberine, phellodendrine and glycyrrhizic acid.
The antibacterial MIC of the phellodendron extract, the licorice extract, the berberine, the phellodendrine and the glycyrrhizic acid against acne related pathogenic bacteria is measured, and the specific experimental results are shown in Table 1:
| MIC(mg·mL-1) | propionibacterium acnes | Staphylococcus aureus | Staphylococcus epidermidis |
| Cortex Phellodendri extract | 0.25 | 1 | 1 |
| Berberine | 0.125 | 1 | 1 |
| Phellodendrine | 0.5 | 1 | 1 |
| |
1 | 2 | 2 |
| |
1 | 1 | 1 |
TABLE 1
According to the bacteriostatic MIC data, the phellodendron extract and the monomer thereof, and the licorice extract and the monomer thereof have excellent bacteriostatic ability against acne related pathogenic bacteria, and the bacteriostatic ability of the phellodendron extract and the monomer thereof can be improved after the phellodendron extract and the monomer thereof are compounded, so as to better illustrate the compounding result thereof, the following description is further provided by combining with specific examples.
Example 1:
the phellodendron and liquorice acne-removing plant composition comprises the following components in parts by weight: 10 parts of phellodendron extract; 90 parts of licorice extract; the sum of the parts by weight of the plant extracts is 100 parts.
The preparation process of the phellodendron extract comprises the following steps: weighing a proper amount of dried phellodendron amurense medicinal materials, crushing, adding into a pure water solution, wherein the material-to-liquid ratio of the dried phellodendron amurense medicinal materials to the pure water is 1:10kg/L, carrying out reflux extraction for 2 times at the temperature of 100 ℃, combining the two extracting solutions, and sequentially carrying out reduced pressure concentration and water bath evaporation to dryness to obtain the phellodendron amurense extract.
The preparation process of the licorice extract comprises the following steps: weighing a proper amount of dried licorice, crushing, adding into 50% ethanol/water mixed liquor by volume fraction, carrying out reflux extraction for 3 times at 75 ℃ with the material-liquid ratio of the dried licorice and the ethanol/water mixed liquor being 1:20kg/L, combining the obtained extract, and sequentially carrying out reduced pressure concentration and water bath evaporation to obtain the licorice extract.
Example 2:
the phellodendron and liquorice acne-removing plant composition comprises the following components in parts by weight: 40 parts of phellodendron extract; 60 parts of licorice extract; the sum of the parts by weight of the plant extracts is 100 parts.
The preparation process of the phellodendron extract comprises the following steps: weighing a proper amount of dried phellodendron amurense medicinal materials, crushing, adding into a pure water solution, wherein the material-to-liquid ratio of the dried phellodendron amurense medicinal materials to the pure water is 1:20kg/L, carrying out reflux extraction for 3 times at the temperature of 100 ℃, combining the two extracting solutions, and sequentially carrying out reduced pressure concentration and water bath evaporation to dryness to obtain the phellodendron amurense extract.
The preparation process of the licorice extract comprises the following steps: weighing a proper amount of dried licorice, crushing, adding into an ethanol/water mixed solution with the volume fraction of 70%, wherein the material-liquid ratio of the dried licorice to the ethanol/water mixed solution is 1:10kg/L, carrying out reflux extraction for 2 times at the temperature of 75 ℃, combining the obtained extracting solutions, and sequentially carrying out reduced pressure concentration and water bath evaporation to dryness to obtain the licorice extract.
Example 3:
the phellodendron and liquorice acne-removing plant composition comprises the following components in parts by weight: 90 parts of phellodendron extract; 10 parts of licorice extract; the sum of the parts by weight of the plant extracts is 100 parts.
The preparation process of the phellodendron extract comprises the following steps: weighing a proper amount of dried phellodendron amurense medicinal materials, crushing, adding into a pure water solution, wherein the material-to-liquid ratio of the dried phellodendron amurense medicinal materials to the pure water is 1:10kg/L, carrying out reflux extraction for 2 times at the temperature of 100 ℃, combining the two extracting solutions, and sequentially carrying out reduced pressure concentration and water bath evaporation to dryness to obtain the phellodendron amurense extract.
The preparation process of the licorice extract comprises the following steps: weighing a proper amount of dried licorice, crushing, adding into 40% ethanol/water mixed liquor by volume fraction, carrying out reflux extraction for 3 times at 75 ℃ with the material-liquid ratio of the dried licorice and the ethanol/water mixed liquor being 1:20kg/L, combining the obtained extract, and sequentially carrying out reduced pressure concentration and water bath evaporation to obtain the licorice extract.
The plant compositions obtained in the examples were tested for inhibitory MIC:
in order to verify the bacteriostatic effect of the phellodendron extract and the licorice extract on acne-related pathogenic bacteria after the phellodendron extract and the licorice extract are compounded, bacteriostatic MIC determination of the acne-related pathogenic bacteria is performed on the phellodendron licorice acne-removing plant composition obtained in the above examples 1-3, and specific experimental results are shown in table 2:
TABLE 2
According to the test data in table 2, it can be known that the bacteriostatic efficacy against acne-related pathogenic bacteria is improved to different degrees by compounding the phellodendron extract and the licorice extract.
Application example:
in order to better verify the antibacterial effect of the phellodendron and liquorice acne-removing plant composition with the function of inhibiting acne-related pathogenic bacteria, which is obtained in examples 1-3, the phellodendron and liquorice acne-removing plant composition is applied to actual moisturizing cream and moisturizing lotion, the mass of the phellodendron and liquorice acne-removing plant composition is 0.3-0.5% of the total mass of cosmetics, and all the raw materials are in parts by mass.
The phellodendron and liquorice acne-removing plant composition obtained in the embodiment 1-3 is applied to moisturizing cream, and the formula of each component of the moisturizing cream is shown in table 3:
| name of raw materials | Application example 1 | Application example 2 | Application example 3 |
| Acrylic acid (esters) | 0.03 | 0.03 | 0.03 |
| Polydimethylsiloxane | 1.00 | 1.00 | 1.00 |
| Xanthan acid | 0.02 | 0.02 | 0.02 |
| Hyaluronic acid sodium salt | 0.02 | 0.02 | 0.02 |
| Hyaluronic acid | 0.02 | 0.02 | 0.02 |
| Tridecyl alcohol trimellitate | 2.00 | 2.00 | 2.00 |
| Butyrospermum parkii fruit resin | 2.00 | 2.00 | 2.00 |
| Prinsepia utilis Royle oil | 0.10 | 0.10 | 0.10 |
| Glycerol stearate | 0.10 | 0.10 | 0.10 |
| Purslane extract | 0.12 | 0.12 | 0.12 |
| Aminomethyl propanol | 0.10 | 0.10 | 0.10 |
| Example 1A composition was prepared | 0.30 | - | - |
| Example 2 preparation of a composition | - | 4.00 | - |
| Example 3A composition was prepared | - | - | 5.00 |
| Water (W) | 94.19 | 94.09 | 93.99 |
TABLE 3
Example 1～3, the phellodendron bark and liquorice acne-removing plant composition is applied to the skin lotion, and the formula of each component of the skin lotion is shown in table 4:
| name of raw materials | Application example 4 | Application example 5 | Application example6 |
| Glycerol | 3.00 | 3.00 | 3.00 |
| Glycerol polyether-26 | 2.00 | 2.00 | 2.00 |
| Hydroxyethyl cellulose | 0.10 | 0.10 | 0.10 |
| Hyaluronic acid sodium salt | 0.10 | 0.10 | 0.10 |
| Beta-glucan | 0.12 | 0.12 | 0.12 |
| Purslane extract | 0.10 | 0.10 | 0.10 |
| Example 1A composition was prepared | 0.30 | - | - |
| Example 2 preparation of a composition | - | 0.40 | - |
| Example 3A composition was prepared | - | - | 0.50 |
| Water (W) | 94.28 | 94.18 | 94.08 |
TABLE 4
Application examples 1-3 of the phellodendron-liquorice acne-removing plant composition applied to moisturizing cream are prepared according to the formula in table 3.
Application examples 4-6 of the phellodendron-liquorice acne-removing plant composition applied to skin moistening water are prepared according to the formula in the table 4.
The sources of the above raw materials are shown in table 5:
TABLE 5
Antibacterial testing experiment:
in order to verify the efficacy of the phellodendron and liquorice acne-removing plant composition in inhibiting acne-related pathogenic bacteria, the antibacterial performance of application examples 1-6 of the invention is determined according to GB15979-2002 'appendix C4 of hygienic Standard for Disposable sanitary articles' appendix C4 'test method for antibacterial performance of dissoluble antibacterial (bacteriostatic) products', and the specific experimental method is as follows:
preparing a bacterial suspension: and (3) eluting the 72h culture of the propionibacterium acnes by using 0.03mol/L phosphate buffer solution to prepare a bacterial suspension with OD600 equal to 0.25 (the bacterial suspension preparation method is still applicable after the propionibacterium acnes is replaced by staphylococcus epidermidis and staphylococcus aureus).
1g of the samples obtained in application examples 1-6 are respectively taken, 0.1mL of the bacterial suspension is added for uniform coating and mixing, 0.03mol/L of phosphate buffer solution is accurately added for uniform mixing after timing for 20min, 3 dilutions are taken, 1mL of the dilutions are respectively taken and placed in 2 parallel plates, and a corresponding culture medium is added for 24h under the anaerobic condition at 37 ℃ (in the method, the culture condition is changed into the aerobic culture condition at 25 ℃, namely, the method is suitable for staphylococcus epidermidis and staphylococcus aureus), and the bacterial colonies are counted. The above experiment was repeated 3 times, and the average value was taken to set a blank control group.
The method for calculating the bacteriostasis rate comprises the following steps: and X is (A-B)/A multiplied by 100 percent, wherein X is the bacteriostasis rate, A is the average recovered colony number of the control sample, and B is the average recovered colony number of the test sample.
Evaluation criteria for efficacy: (1) the bacteriostasis rate is more than or equal to 90 percent, and is obvious and effective; (2) the bacteriostasis rate is 50 to 90 percent effective; (3) the bacteriostasis rate is less than 50 percent, which is ineffective.
Through the above-mentioned experimental antibacterial test, test application example 1 ~ 6 gained the antibacterial effect of sample, and specific antibacterial test experimental result is shown in table 6:
| test sample | Application example 1 | Application example 2 | Application example 3 | Application example 4 | Application example 5 | Application example 6 |
| Propionibacterium acnes inhibition rate (%) | 98 | 94 | 91 | 90 | 92 | 96 |
| Evaluation of efficacy | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective |
| Staphylococcus aureus inhibitory rate (%) | 92 | 97 | 94 | 95 | 93 | 91 |
| Evaluation of efficacy | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective |
| Staphylococcus epidermidis bacteriostasis rate (%) | 97 | 95 | 93 | 95 | 96 | 93 |
| Evaluation of efficacy | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective | Is remarkably effective |
TABLE 6
As can be seen from the antibacterial test results obtained in table 6, the cosmetics containing the phellodendron-glycyrrhiza acne-removing plant composition obtained in application examples 1 to 6 all have significant and effective antibacterial ability, which indicates that the phellodendron-glycyrrhiza acne-removing plant composition can significantly inhibit the proliferation of pathogenic bacteria related to acne, thereby achieving the treatment and prevention of acne, and having good market value and application prospect.
In conclusion, the phellodendron and liquorice acne-removing plant composition is prepared by reasonably compounding the phellodendron extract and the liquorice extract, and has remarkable and effective acne-removing capability; the adopted plant medicinal materials are easy to obtain, the golden cypress has the effects of clearing heat and drying dampness, purging fire and removing steam, detoxifying and treating sore, and the liquorice has the effects of clearing heat and detoxifying and preventing and treating tumor, and has very good potential application value; the prepared phellodendron and liquorice acne-removing plant composition has the excellent characteristics of high safety, obvious inhibition on acne and the like, and can effectively inhibit the proliferation of acne-related pathogenic bacteria, so that the treatment, prevention and treatment of the acne are achieved; the antibacterial performance is obviously effective when the antibacterial agent is added into the actual cosmetic formula and the antibacterial performance is measured according to GB15979-2002 hygienic Standard for Disposable sanitary articles appendix C4 'test method for antibacterial performance of dissolution antibacterial (inhibiting) products'.
It should be noted that the above-mentioned embodiments are only used for illustrating the present invention and are not used as limitations for those skilled in the art and related arts, and changes and modifications to the above-mentioned embodiments are within the scope of the claims of the present invention as long as they are within the spirit and scope of the present invention.
Claims (9)
1. The phellodendron and liquorice acne-removing plant composition is characterized by comprising the following components in parts by weight:
10-90 parts of a phellodendron extract;
10-90 parts of a licorice extract;
the sum of the weight parts of the phellodendron extract and the liquorice extract is 100 parts.
2. The phellodendron-liquorice acne-removing plant composition as claimed in claim 1, wherein the preparation process of the phellodendron extract comprises the following steps: weighing dry medicinal materials of golden cypress, crushing, adding the medicinal materials into a pure water solution, performing reflux extraction for 1-4 times, mixing obtained extracting solutions, and performing reduced pressure concentration and water bath evaporation to dryness in sequence to obtain a golden cypress extract;
the ratio of the materials to the liquid of the dry medicinal materials of the golden cypress to the pure water is 1: 6-1: 20kg/L during each extraction.
3. The phellodendron-liquorice acne-removing plant composition as claimed in claim 1 or 2, wherein the content of berberine in the phellodendron extract is not less than 9%, and the content of phellodendrine is not less than 3%.
4. The phellodendron-liquorice acne-removing plant composition as claimed in claim 1, wherein the preparation process of the liquorice extract comprises the following steps: weighing dry licorice, crushing, adding the crushed dry licorice into an alcohol/water mixed solution, performing reflux extraction for 1-4 times, mixing obtained extracting solutions, and performing reduced pressure concentration and water bath evaporation to dryness in sequence to obtain a licorice extract;
in each extraction, the material-liquid ratio of the dry licorice and the alcohol/water mixed solution is 1: 6-1: 20 kg/L;
the alcohol in the alcohol/water mixed solution is one or more of methanol, ethanol, pentanediol and butanediol;
the volume fraction of the alcohol in the alcohol/water mixed solution is 30-95%.
5. The phellodendron-liquorice acne-removing plant composition as claimed in claim 1 or 4, wherein the glycyrrhizic acid content in the liquorice extract is not less than 5% by mass.
6. The application of the phellodendron-liquorice acne-removing plant composition as claimed in claim 1, wherein the phellodendron-liquorice acne-removing plant composition is added into cosmetics, and the mass of the phellodendron-liquorice acne-removing plant composition is 0.3-0.5% of the total mass of the cosmetics.
7. The application of the phellodendron-liquorice acne-removing plant composition as claimed in claim 6, wherein the cosmetic is moisturizing cream or moisturizing lotion.
8. The application of the phellodendron-liquorice acne-removing plant composition as claimed in claim 7, wherein the moisturizing cream comprises the following components in parts by weight: 0.01-0.05 part of acrylic acid (ester), 0.8-1.2 parts of polydimethylsiloxane, 0.01-0.03 part of xanthan gum, 0.01-0.03 part of sodium hyaluronate, 0.01-0.03 part of hyaluronic acid, 1-3 parts of tridecanol trimellitate, 1-3 parts of shea butter, 0.05-0.15 part of prinsepia utilis royle oil, 0.5-0.15 part of glycerol stearate, 0.04-0.2 part of purslane extract, 0.05-0.15 part of aminomethyl propanol, 0.3-0.5 part of phellodendron and liquorice acne-removing plant composition and the balance of water;
the sum of the weight parts of the components of the moisturizing cream is 100 parts.
9. The application of the phellodendron-liquorice acne-removing plant composition as claimed in claim 7, wherein the emollient water comprises the following components in parts by weight: 1-5 parts of glycerol, 261-5 parts of glyceryl polyether, 0.03-0.08 part of hydroxyethyl cellulose, 0.01-0.05 part of sodium hyaluronate, 0.04-0.2 part of beta-glucan, 0.04-0.2 part of purslane extract, 0.3-0.5 part of phellodendron and liquorice acne-removing plant composition and the balance of water;
the sum of the parts by weight of the components of the emollient water is 100 parts.
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