CN115671167A - Traditional Chinese medicine composition with acne removing effect, traditional Chinese medicine preparation, and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition with acne removing effect, traditional Chinese medicine preparation, and preparation method and application thereof Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a traditional Chinese medicine composition with an acne removing effect, a traditional Chinese medicine preparation, a preparation method and application thereof, and relates to the field of daily cosmetics. The traditional Chinese medicine composition with the acne removing effect comprises the following raw materials in parts by weight: 5-50 parts of total paeony glycoside, 3-30 parts of liquorice extract, 2-25 parts of buckeye extract and 1-10 parts of red deer blood extract. The active ingredients in the traditional Chinese medicine composition with the acne removing effect are matched and used together, so that the traditional Chinese medicine composition has no toxic or side effect and adverse reaction, takes effect quickly, and achieves the effects of removing acne, diminishing inflammation, improving skin sensitivity and repairing damaged skin through multiple ways and multiple layers.
Description
Technical Field
The invention relates to the field of daily cosmetics, in particular to a traditional Chinese medicine composition with an acne removing effect, a traditional Chinese medicine preparation, and a preparation method and application thereof.
Background
Acne, also known as "whelk", is one of the typical skin problems that 80% of people between the ages of 11 and 30 suffer from. Acne is a chronic inflammation of the sebaceous glands of the follicles resulting from the blockage of the follicles and the sebaceous glands and the failure of the normal outflow of sebum, often occurring in areas with dense sebaceous glands and causing various lesions such as comedones, papules, cysts, nodules, etc. Many studies have shown that abnormal androgen secretion, abnormal follicular sebaceous gland duct keratosis, stimulation by pathogenic microorganisms, inflammatory lesions, immune responses, etc. are the major factors responsible for the formation of "pox". In order to eliminate acne, comedones, consumers generally purchase cosmetics that claim to have an anti-acne, acaricidal and anti-comedones effect.
At present, the types of acne-removing cosmetics seen on the market are various, and the supervision of the components and the safety of the acne-removing cosmetics is necessary. The anti-acne cosmetics are mainly prepared by using hormone antagonists, tretinoin medicaments, antibiotics and the like to treat the skin diseases, and although the medicaments have obvious curative effects, the toxic effects are not small, such as allergy, kidney function damage, even deformity and the like. Therefore, the plant extract component for safely and effectively treating the acne becomes a development hotspot of acne-removing cosmetics, and a cosmetic formula which can improve the natural tolerance threshold of skin, effectively treat the acne, has good acne-removing and inflammation-diminishing effects and does not generate other side effects is needed in the field.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide a traditional Chinese medicine composition with an acne removing effect, a traditional Chinese medicine preparation, and a preparation method and application thereof. The traditional Chinese medicine composition with the acne removing effect provided by the invention is quick in effect, and can achieve the effects of removing acne, diminishing inflammation, improving skin sensitivity and repairing damaged skin through multiple ways and multiple layers.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention provides a traditional Chinese medicine composition with an acne removing effect, which comprises the following raw materials in parts by weight: 5-50 parts of total paeony glycoside, 3-30 parts of licorice extract, 2-25 parts of buckeye extract and 1-10 parts of red deer blood extract.
Preferably, the traditional Chinese medicine composition with acne removing efficacy comprises the following raw materials in parts by weight: 10-30 parts of total paeony glycoside, 10-20 parts of licorice extract, 5-20 parts of buckeye extract and 3-8 parts of red deer blood extract.
Preferably, the total paeoniflorin is obtained by adsorbing a paeony alcohol extract by activated carbon.
Preferably, the licorice extract is an aqueous extract of licorice; the buckeye seed extract is an alcohol extract of buckeye seed.
Preferably, the red deer blood extract is a zymolyte of red deer blood after enzymolysis by papain.
The invention provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
drying and crushing the peony extract, the licorice extract, the buckeye extract and the red deer blood extract, and uniformly mixing to obtain the traditional Chinese medicine composition with the acne removing effect.
The invention also provides a traditional Chinese medicine preparation with the acne removing effect, which contains the traditional Chinese medicine composition with the acne removing effect.
Preferably, the formulation is a powder, a plaster, an ointment, a lotion, a mask or a gel.
The invention also provides application of the traditional Chinese medicine composition with the acne removing effect or the traditional Chinese medicine preparation with the acne removing effect in cosmetics or medicines for treating skin diseases.
The traditional Chinese medicine composition with the acne removing effect comprises radix paeoniae total glycosides, liquorice extract, buckeye extract and red deer blood extract; wherein, the peony total glycosides show antioxidant activity and hormone-like action; simultaneously, the release of inflammatory mediators can be inhibited, and the anti-inflammatory effect and the broad-spectrum antibacterial effect are shown; glycyrrhizic acid in the licorice extract can obviously increase the generation of active oxygen and nitrogen, increase the expression of antibacterial genes, activate macrophages and enhance the killing capacity of phagocytes and salmonella; aescin in Chinese buckeye seed can reduce the release of proinflammatory factors, improve the tolerance threshold of skin, effectively repair skin flushing, local swelling, acne and the like, and effectively reduce skin inflammatory reaction; the red deer blood after enzymolysis can promote the synthesis and activation of superoxide dismutase, reduce the formation of free radicals, protect cell membranes, improve the functions of facial skin tissues, enhance metabolism and delay aging. The traditional Chinese medicine composition provided by the invention embodies the synergistic effect of traditional Chinese medicines, and has a more remarkable acne removing effect compared with any single extract.
The traditional Chinese medicine composition with the acne removing effect is a composition prepared by combining peony total glycosides, licorice extract, buckeye extract and red deer blood extract for the first time and is used for cosmetics. The formula contains paeoniflorin, flavone, hormone-like substances, vitamins and amino acid compounds, has an acne removing effect, is beneficial to repairing damaged cells and slowing down the metabolism of free radicals on cell tissues, thereby achieving the effects of smoothing facial wrinkles, fading dark circles, nourishing, repairing, regenerating, removing wrinkles, removing freckles, removing yellow and removing darkness. Meanwhile, the composition provided by the invention has the advantages of simple preparation process, low price, exact curative effect, no toxic or side effect, convenience in use, scientific dosage form and suitability for wide application.
Detailed Description
The invention provides a traditional Chinese medicine composition with an acne removing effect, which comprises the following raw materials in parts by weight: 5-50 parts of total paeony glycoside, 3-30 parts of licorice extract, 2-25 parts of buckeye extract and 1-10 parts of red deer blood extract.
In the invention, the traditional Chinese medicine composition with the acne removing effect comprises 5-50 parts by weight of total paeoniflorin, preferably 10-30 parts by weight, and more preferably 25 parts by weight.
In the invention, the total paeoniflorin is preferably extracted and purified from dried roots of Paeonia lactiflora PalL (Paeonia lactiflora PalL) of Ranunculaceae, and the total glycoside compound content in the extract is about 86 percent; the peony is bitter in taste and slightly cold, enters liver meridian, and has the effects of clearing heat and cooling blood, and activating blood and dissolving stasis. Modern researches show that the red paeony root contains various chemical components such as terpenes, glycosides and flavonoids thereof, and has various pharmacological actions and efficacies such as liver protection, nerve protection, thrombus resistance, oxidation resistance, endotoxin resistance and the like.
In the invention, the total paeoniflorin is preferably obtained by adsorbing red paeony root alcohol extract by activated carbon; the preparation of the total paeoniflorin preferably comprises the following steps: extracting radix Paeoniae Rubra with ethanol under reflux for 2-3 times, each for 2-3 hr, filtering, mixing filtrates, adding active carbon, stirring, filtering, recovering ethanol from filtrate, concentrating, filtering, drying filter cake, pulverizing into fine powder, and sieving with 60 mesh sieve. In the present invention, the mass fraction of ethanol in the reflux extraction is preferably 30 to 75%, more preferably 35 to 70%. In the invention, the activated carbon is preferably medicinal activated carbon; the addition amount of the medicinal active carbon is preferably 0.1-2.0% of the volume of the filtrate, and more preferably 0.5-1.8% of the volume of the filtrate.
In the invention, the traditional Chinese medicine composition with the acne removing effect comprises 3-30 parts by weight of liquorice extract, preferably 10-20 parts by weight, and more preferably 15 parts by weight. In the invention, the licorice extract is preferably an aqueous extract of licorice; the Glycyrrhrizae radix is preferably dried root and rhizome of Glycyrrhiza uralensis Fisch, glycyrrhiza inflata Bat or Glycyrrhiza glabra L of Leguminosae. In the invention, the liquorice has the effects of tonifying spleen and qi, clearing away heat and toxic materials and treating carbuncle and sore toxin, main chemical components mainly comprise triterpenoid saponins and flavonoids, and also comprise coumarin, alkaloid, amino acid, volatile components, polysaccharide and the like.
In the present invention, the preparation of the licorice extract preferably comprises the steps of: mixing Glycyrrhrizae radix with water, decocting, filtering to obtain filtrate, concentrating, drying, and pulverizing to obtain Glycyrrhrizae radix extract. In the present invention, the mass ratio of the licorice to water is preferably 1: (6-12), more preferably 1: (8-10). In the present invention, the number of times of decoction is preferably 1 to 3 times, and the time for each decoction is preferably 1 to 3 hours.
In the invention, the Chinese medicinal composition with the acne removing effect comprises 2-25 parts by weight of buckeye extract, preferably 5-20 parts by weight of buckeye extract, and more preferably 10 parts by weight of buckeye extract. In the invention, the buckeye extract is preferably an alcohol extract of buckeye; the buckeye mainly comprises aescin, flavonoid compounds, concentrated tannic acid, sterol and the like, can play a synergistic effect with phytosterol and phospholipid to promote the micro-vascular circulation of skin, smooth out the lassitude trace, relieve diffuse red blood filaments, and have the effects of effectively improving the expansion phenomenon of facial capillaries, maintaining the integrity of blood vessel walls, reducing the vascular permeability, preventing the water accumulation in tissues, quickly eliminating the heavy feeling and pressure caused by local edema and enabling skin tissues to be dense and elastic.
In the present invention, the preparation of the buckeye extract preferably comprises the following steps: extracting semen Aesculi with ethanol, filtering to obtain filtrate, concentrating, drying, and pulverizing into fine powder to obtain semen Aesculi extract. In the invention, the mass fraction of ethanol in the extraction is preferably 40-80%, and more preferably 45-75%; the extraction times are preferably 1-3 times; the time for each extraction is preferably 1 to 3 hours. In the present invention, the particle size of the fine powder is preferably not less than 60 mesh. In the invention, the main component of the salmons extract is preferably aescin.
In the invention, the traditional Chinese medicine composition with the acne removing effect comprises 1-10 parts by weight of red deer blood extract, preferably 3-8 parts by weight, and more preferably 5 parts by weight. In the invention, the red deer blood extract shows antioxidant activity and hormone-like action, and has anti-inflammatory and immunoregulatory effects. The research and application of the red deer blood are promoted by various functions of the red deer blood. Modern scientific research shows that human beings utilize protein by hydrolyzing the protein into small molecular polypeptide for absorption, the invention has special limitation on the specific preparation method of the red deer blood extract, and the red deer blood extract can be prepared by adopting conventional commercially available red deer blood in the field. In the inventionThe preparation of the red deer blood preferably comprises the following steps: the substrate concentration (red deer blood: distilled water) is 1:4 (m/m), and 0.1mol/L H is used 2 SO 4 Or adjusting pH with NaOH to a suitable value, adding papain at 50 deg.C for enzymolysis for 5h, inactivating enzyme at 95 deg.C for 10min, centrifuging at 3500r/min for 20min, collecting supernatant and precipitate, and drying at low temperature. The water content in the red deer blood is 83 percent and the protein content is 18 percent through calculation.
The invention provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
drying and crushing the peony total glycosides, the licorice extract, the buckeye extract and the red deer blood extract, and uniformly mixing to obtain the traditional Chinese medicine composition with the acne removing effect. The method of drying or pulverizing in the present invention is not particularly limited, and a method of drying or pulverizing which is conventional in the art may be employed.
The invention also provides a traditional Chinese medicine preparation with the acne removing effect, which contains the traditional Chinese medicine composition with the acne removing effect. In the invention, the traditional Chinese medicine preparation is preferably powder, plaster, ointment, lotion, emulsion, mask or gel.
The invention also provides application of the traditional Chinese medicine composition with the acne removing effect or the traditional Chinese medicine preparation with the acne removing effect in cosmetics or medicines for treating skin diseases. The method of the present invention is not particularly limited, and any known method may be used.
The invention uses the peony total glycosides, the licorice extract, the buckeye extract and the red deer blood extract to prepare the composition, and the composition can make the subcutaneous tissue thicker, make the skin no longer brittle and sensitive and increase the skin elasticity and toughness by resisting bacteria, resisting skin exudation and inflammation, repairing damaged cells, promoting the regeneration of the skin epithelial tissue, reducing the accumulation of stratum corneum and blocking pores, and enabling the keratinocyte and fibroblast to proliferate.
In order to further illustrate the present invention, the following embodiments are described in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
1. Preparation of total paeoniflorin
Extracting radix Paeoniae Rubra dried root with 40% ethanol under reflux for 2 times, each for 2 hr, filtering, mixing filtrates, adding medicinal activated carbon 1.0% of the medicinal liquid volume, stirring, filtering, recovering ethanol from the filtrate, concentrating, filtering, drying the filter cake, and pulverizing into 60 mesh fine powder.
2. Preparation of licorice extract
Slicing Glycyrrhrizae radix, decocting with 6 times of water for 3 times (each for 2 hr), filtering, mixing filtrates, concentrating, drying, and pulverizing to obtain Glycyrrhrizae radix extract.
3. Preparation of semen Aesculi extract
Crushing semen Aesculi, extracting with 50% ethanol for 3 times (2 hr each time), filtering, mixing filtrates, concentrating, drying, and pulverizing into 60 mesh fine powder to obtain semen Aesculi extract.
4. Preparation method of red deer blood extract
The substrate concentration (deer blood: distilled water) is 1:2 (m/m), and 0.1mol/L H is used 2 Adjusting pH of SO4 or NaOH to appropriate pH value, adjusting protease concentration to 8%, adding papain at 50 deg.C for enzymolysis for 5h, inactivating enzyme at 95 deg.C for 10min, centrifuging at 3500r/min for 20min, collecting supernatant and precipitate, and drying at low temperature.
5. Preparation of traditional Chinese medicine composition with acne removing effect
5 parts of paeony total glycoside, 3 parts of licorice extract, 2 parts of buckeye extract and 1 part of red deer blood extract are uniformly mixed according to a proportion to obtain the traditional Chinese medicine composition with the acne removing effect.
Example 2
1. Preparation of total paeoniflorin
Extracting radix Paeoniae Rubra dried root with 50% ethanol under reflux for 3 times, each for 2 hr, filtering, mixing filtrates, adding medicinal activated carbon 1.0% of the medicinal liquid volume, stirring, filtering, recovering ethanol from the filtrate, concentrating, filtering, drying the filter cake, and pulverizing into fine powder.
2. Preparation of licorice extract
Slicing Glycyrrhrizae radix, decocting with 8 times of water for 3 times (each for 2 hr), filtering, mixing filtrates, concentrating, drying, and pulverizing to obtain Glycyrrhrizae radix extract.
3. Preparation of semen Aesculi extract
Crushing semen Aesculi, extracting with 70% ethanol for 3 times, each for 2 hr, filtering, mixing filtrates, concentrating, drying, and pulverizing into fine powder to obtain semen Aesculi extract.
4. Preparation method of red deer blood extract
The substrate concentration (deer blood: distilled water) is 1:3 (m/m), and 0.1mol/L H is used 2 SO 4 Or adjusting pH with NaOH to a suitable value, adding papain at 50 deg.C for enzymolysis for 5h, inactivating enzyme at 95 deg.C for 10min, centrifuging at 3500r/min for 20min, collecting supernatant and precipitate, and drying at low temperature.
5. Preparation of traditional Chinese medicine composition with acne removing effect
Mixing 50 parts of total paeony glycoside, 30 parts of licorice extract, 20 parts of buckeye extract and 10 parts of red deer blood extract uniformly according to a proportion to obtain the traditional Chinese medicine composition with the acne removing effect.
Example 3
1. Preparation of total paeoniflorin
Extracting radix Paeoniae Rubra dried root with 70% ethanol under reflux for 3 times, each for 2 hr, filtering, mixing filtrates, adding medicinal activated carbon 2.0% of the medicinal liquid volume, stirring, filtering, recovering ethanol from filtrate, concentrating, filtering, drying filter cake, and pulverizing into fine powder.
2. Preparation of licorice extract
Slicing Glycyrrhrizae radix, decocting with 10 times of water for 3 times (each for 2 hr), filtering, mixing filtrates, concentrating, drying, and pulverizing to obtain Glycyrrhrizae radix extract.
3. Preparation of semen Aesculi extract
Crushing semen Aesculi, extracting with 70% ethanol for 3 times (2 hr each time), filtering, mixing filtrates, concentrating, drying, and pulverizing into fine powder to obtain semen Aesculi extract.
4. Preparation method of red deer blood extract
The substrate concentration (deer blood: distilled water) is 1:4 (m/m), and 0.1mol/L H is used 2 SO 4 Or adjusting pH to a proper value with NaOH, adding papain to carry out enzymolysis for 5h at 50 ℃, inactivating enzyme for 10min at 95 ℃, centrifuging for 20min at 3500r/min, collecting supernatant and precipitate, and drying at low temperature, wherein the concentration of the protease is 8%.
5. Preparation of traditional Chinese medicine composition with acne removing effect
Mixing 50 parts of total paeony glycoside, 30 parts of licorice extract, 25 parts of buckeye extract and 10 parts of red deer blood extract uniformly according to a proportion to obtain the traditional Chinese medicine composition with the acne removing effect.
Example 4 bacteriostatic test
The total paeoniflorin mentioned in the pharmacodynamic test is a sample extracted in the step 1 of the example 2; the licorice extract is the sample extracted in step 2 of example 2; the buckeye seed extract is a sample extracted in step 3 of example 2; the red deer blood extract is a sample obtained by enzymolysis in the step 4 of the embodiment 2; the traditional Chinese medicine composition with the acne removing effect is prepared in example 2.
1. Preparation of the bacterial suspension
Propionibacterium acnes and Staphylococcus aureus were selected as test strains.
And taking out the staphylococcus aureus ATCC6538 and the propionibacterium acnes ATCC11827 freezing tubes from a refrigerator at the temperature of-80 ℃, and placing the freezing tubes in a biological safety cabinet until the temperature is restored to the normal temperature. Then inoculating 1 loop, i.e. 10. Mu.L of Staphylococcus aureus solution into 10mLBHI liquid medium, and culturing at 37 ℃ for 48h. Diluting 1-9 mL of 0.85% physiological saline added with 1 ‰ peptone twice, diluting 100 times, and making into 10 times 7 CFU/mL bacterial suspension for use. The Propionibacterium acnes is cultured in an anaerobic culture box at 37 ℃ for 48h by using an improved GAM liquid culture medium added with 5% defibrinated sheep blood, and the other steps are the same as the operation of staphylococcus aureus.
2. Procedure of the test
BHI solid medium was dissolved and returned to 46 ℃ and poured 15-20mL into plates to allow it to solidify. Suck 100. Mu.L of bacterial suspension and spread evenly on the plate with L-shaped spreading rod, wait about 10min to dry. Two oxford cups were held with sterile forceps and placed on the plate and slightly stressed to insert and fix on the medium. Respectively sucking 200 mu L of samples to be detected, adding the samples into two oxford cups, parallelly comparing two flat plates of the same sample, and simultaneously arranging a Blank control flat plate Blank. The plates were incubated at 37 ℃ for 48h and the results were read. Culturing Propionibacterium acnes in anaerobic culture box at 37 deg.C for 48 hr with modified GAM solid culture medium containing 5% defibrinated sheep blood, and performing the same steps.
3. Reading the result
The diameter of the inhibition zone is measured by a vernier caliper, two effective numbers are reserved by taking millimeter (mm) as a unit, and the result is recorded.
The antibacterial performance was evaluated as: the bacteriostatic zone less than 10mm has no obvious bacteriostatic action, the zone more than 10mm and less than 15mm has moderate bacteriostatic action, and the zone more than 15mm has high bacteriostatic action.
4. Sample preparation
The total paeony glycoside, the licorice extract, the buckeye extract, the red deer blood extract and the traditional Chinese medicine composition are prepared into a sample containing 5g of Chinese herbal medicine per mL, and the results are shown in Table 1.
TABLE 1 antibacterial test results of the Chinese medicinal composition of the present invention
The Chinese medicinal composition has obvious bacteriostatic activity, and compared with single components, the Chinese medicinal composition has stronger bacteriostatic activity.
Example 5 Experimental study on acne in rats
90 healthy Wistar rats with the weight of 180g +/-20 g are taken and divided into 9 groups of 10 rats, and the specific arrangement is shown in Table 2.
TABLE 2 animal groups
| Group of | Animal number (only) | Modeling of artificial infection | Therapeutic agents |
| Normal control group | 10 | Whether or not | No treatment |
| Model control group | 10 | Is that | No treatment |
| Positive control group | 10 | Is that | Clindamycin metronidazole |
| Radix Paeoniae Total glycoside | 10 | Is that | Treatment of |
| Glycyrrhiza extract | 10 | Is that | Treatment of |
| Semen Aesculi extract | 10 | Is that | Treatment of |
| Cervus Elaphus blood extract | 10 | Is that | Treatment of |
| The Chinese medicinal composition of the invention | 10 | Is that | Treatment of |
1. Preparation of rat skin acne model
Except for the blank control group, the rats of the other groups were anesthetized with ether, and 50. Mu.L of Propionibacterium acnes suspension (6X 10) was injected into the rats using a syringe 8 CFU·mL -1 ) The injections were made into the auricle skin of the right ear, and the thickness of the auricle of the rat was measured every 24 hours for 3 days continuously after the injections. The skin of the normal control group rats was not treated.
2. Method of administration
The skin of the rats in the normal control group and the model control group is not administrated, and other adjuvant treatment is not carried out; the positive control is smeared with clindamycin and metronidazole liniment, and other components are respectively smeared with 50mg/mL of total paeony glycoside, licorice extract, buckeye extract, red deer blood extract and the traditional Chinese medicine composition. On the 14 th day of continuous administration, rats were sacrificed 24 hours later, and serum and rat auricles were collected.
3. General lifestyle status observations in rats
During the administration period, the physiological and behavioral characteristics, wound morphology and animal death of each group of animals were observed and recorded before daily administration.
In the whole experimental process, the body hair of the rats in the normal control group is smooth, the diet is normal, the reaction is quick, and the activity is good. Except for the normal control group, after the rats in other groups are infected for 4 days continuously, auricle swelling parts are picked by using a sterile needle head and are plated on a flat plate for culture, and the strain growth proves that propionibacterium acnes, the area of hair follicles is enlarged, keratinized substances are in infundibulum parts, and sebaceous glands are proliferated, thereby indicating that the molding is successful.
In the administration process, the model control group can catch ears, reduce food intake, and have aggregation and overlapping phenomena. The positive control group and the experimental group of rats begin to recover food intake and increase activity along with the prolonging of treatment time, only a few rats have aggregation and overlapping phenomena, the quantity of keratinized substances is reduced from large to small, and the keratinocyte hyperplasia is not increased any more.
The results show that the positive control medicament and the experimental medicament can improve the living behavior of the model rat and can promote the acne-removing efficacy.
4. Observation of degree of swelling (%) of auricle of rat auricle acne model
Auricle thickness was measured before injection of propionibacterium acnes and after the 3 rd and 14 th days of administration using a micrometer thickness gauge. The auricle swelling rate was calculated as the auricle swelling rate (%) = (auricle thickness after injection-auricle thickness before injection)/auricle thickness before injection × 100%, and the experimental results are shown in table 3.
Table 3 swelling degree (%) of rat auricle acne model auricle
Note: p < 0.05, P < 0.01, P <0.001
It can be seen that on day 3 of inoculation, there was a very significant difference in the degree of swelling of the auricle in rats compared to the model group (P < 0.001), and there was no difference in the degree of swelling of the auricle in rats compared to the other treatment groups and the model group, indicating that the acne model was successful and maintained for a long time. After 14 days of administration, compared with the model group, each treatment group obviously inhibits the auricle swelling degree of an acne model rat, and the traditional Chinese medicine composition has the best effect and better effect than a single extract.
Example 6
Clinical trial
Evaluation target: 70 young people with the age of 15-30 years old, wherein 35 male and 35 female are randomly and averagely divided into 7 groups of 10 people;
evaluating the basic condition of the skin of a subject: acne rosacea, acne vulgaris;
the evaluation method comprises the following steps: the above-mentioned Chinese medicinal compositions for removing acne effects prepared in examples 1 to 3 and comparative examples 1 to 4 were used for each group, and the experiment was carried out for 28 days in total, and the effect of 28 days was examined. Wherein, the comparative example 1 is composed of single component peony total glycoside, the comparative example 2 is composed of single component licorice extract, the comparative example 3 is composed of single component buckeye extract, and the comparative example 4 is composed of single component red deer blood extract.
The test method comprises the following steps: after the basic skin care step, a proper amount of the acne-removing traditional Chinese medicine composition is smeared, the smearing amount is increased at the position of acne, the smearing amount is increased once every morning and evening, each group only uses one acne-removing traditional Chinese medicine composition, and comprehensive evaluation is carried out by referring to Chinese acne treatment guidelines.
And (3) healing: the lesions all subsided, with only minor pigmentation and mild scars;
the effect is shown: the skin damage relieving degree is more than or equal to 70 percent, and various clinical symptoms are obviously improved:
the method has the following advantages: the skin damage relieving degree is more than or equal to 30 percent, and various clinical symptoms have obvious improvement trend but do not disappear;
and (4) invalidation: the skin damage relieving degree is less than 30 percent, and various clinical symptoms are not improved or have obvious aggravation trend.
Statistical test results after 28 days table 4 below:
TABLE 4 statistical table of acne removing abilities of different traditional Chinese medicine compositions
As can be seen from the table, examples 1 to 3 exhibited better acne removing effects than comparative example 1. Acne heals more, with the acne removal effect of example 3 being most prominent. The results prove that the acne-removing traditional Chinese medicine composition prepared by the invention has a good inhibition effect on acne and pox, contains natural components, and is safe and non-irritating.
The embodiment shows that the Chinese medicinal composition with the acne removing effect provided by the invention has the synergistic effect of the total paeoniflorin, the licorice extract, the buckeye extract and the deer blood extract, has more obvious activity than a single extract, and has the effects of repairing skin and treating skin flushing, local swelling and acne.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (9)
1. The traditional Chinese medicine composition with the acne removing effect is characterized by comprising the following raw materials in parts by weight: 5-50 parts of total paeony glycoside, 3-30 parts of licorice extract, 2-25 parts of buckeye extract and 1-10 parts of red deer blood extract.
2. The traditional Chinese medicine composition according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 10-30 parts of total paeony glycoside, 10-20 parts of liquorice extract, 5-20 parts of buckeye extract and 3-8 parts of red deer blood extract.
3. The composition of claim 1 or 2, wherein the total paeoniflorin is an extract of paeonol obtained by adsorption with activated carbon.
4. The traditional Chinese medicine composition according to claim 1 or 2, wherein the licorice extract is an aqueous extract of licorice; the buckeye seed extract is an alcohol extract of buckeye seed.
5. The traditional Chinese medicine composition as claimed in claim 1 or 2, wherein the red deer blood extract is a zymolyte of red deer blood after enzymolysis by papain.
6. The method for preparing the traditional Chinese medicine composition of any one of claims 1 to 5, which is characterized by comprising the following steps:
drying and crushing the peony total glycosides, the licorice extract, the buckeye extract and the red deer blood extract, and uniformly mixing to obtain the traditional Chinese medicine composition with the acne removing effect.
7. A Chinese medicinal preparation with acne removing effect, which is characterized by comprising the Chinese medicinal composition with acne removing effect of any one of claims 1 to 6.
8. The traditional Chinese medicine preparation with the acne removing effect according to claim 7, wherein the preparation is powder, plaster, ointment, lotion, emulsion, mask or gel.
9. Use of the traditional Chinese medicine composition with acne removing efficacy according to any one of claims 1 to 5 or the traditional Chinese medicine preparation with acne removing efficacy according to any one of claims 8 to 9 in cosmetics or medicines for treating skin diseases.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112972328A (en) * | 2021-02-25 | 2021-06-18 | 云南贝泰妮生物科技集团股份有限公司 | Phellodendron and liquorice acne-removing plant composition and application thereof |
| CN114272182A (en) * | 2022-02-17 | 2022-04-05 | 长春仁人药业有限公司 | Preparation method and application of traditional Chinese medicine composition with skin repairing effect |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112972328A (en) * | 2021-02-25 | 2021-06-18 | 云南贝泰妮生物科技集团股份有限公司 | Phellodendron and liquorice acne-removing plant composition and application thereof |
| CN114272182A (en) * | 2022-02-17 | 2022-04-05 | 长春仁人药业有限公司 | Preparation method and application of traditional Chinese medicine composition with skin repairing effect |
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| Title |
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| 宋胜利等: "商业性活鹿无菌采血技术与鹿血资源开发研究进展", 《时珍国医国药》, vol. 12, no. 3, pages 259 - 261 * |
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