CN111281966B - Composition containing earthworm protein and preparation method and application thereof - Google Patents
Composition containing earthworm protein and preparation method and application thereof Download PDFInfo
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- CN111281966B CN111281966B CN202010100027.3A CN202010100027A CN111281966B CN 111281966 B CN111281966 B CN 111281966B CN 202010100027 A CN202010100027 A CN 202010100027A CN 111281966 B CN111281966 B CN 111281966B
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1767—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
- A61K31/355—Tocopherols, e.g. vitamin E
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/732—Chaenomeles, e.g. flowering quince
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
Abstract
The invention discloses a composition containing earthworm protein, a preparation method and application thereof, wherein the effective components in the formula comprise the following components in parts by weight: 10-15 parts of earthworm freeze-dried powder or earthworm dry powder, 5-8 parts of trollius chinensis bunge extract, 5-8 parts of pawpaw extract, 5-8 parts of centella asiatica extract, 3-5 parts of vitamin E and 5-8 parts of humectant. The invention adopts earthworm protein as a main active component, and extracts of trollius chinensis, pawpaw, centella asiatica and the like are matched to be used as the active components together according to corresponding proportion, so that the earthworm protein can be used for repairing erythroserin acne and has the effect of inhibiting the scar hyperplasia of cystic abscess or polymeric abscess. The earthworm protein composition can prevent pigmentation and scars of the skin of a patient suffering from the hormonal acne in the using process.
Description
Technical Field
The invention relates to the field of skin treatment and skin care, in particular to a composition containing earthworm protein and a preparation method and application thereof.
Background
"hormonal face" is a kind of acne medicamentosa, also called hormonal acne, and is a serious skin disease caused by toxic and side effects of hormones due to intermittent or long-term abuse of hormone ointment or cosmetic cosmetics containing hormones. The hormone face is different from common skin sensitivity or allergy, and not only seriously destroys the normal physiological structure and function of the skin, but also influences the whole body health of a patient, and brings great trouble to treatment, wherein nodular acne and cystic acne are often combined with infection, and local hair follicles can be infected. Pigmentation appears frequently during the treatment process; skin aging, tightness; thickening and lengthening fine hair; the symptoms of burning, itching, pain, tightness and the like are felt.
At present, most of external skin care products on the market have insufficient repair capability on skin injury caused by various hormones, cannot achieve the effects of specifically diminishing inflammation, resisting bacteria, promoting skin regeneration and reconstructing a natural barrier of the skin, and cannot solve the problems of pockmark formation and lifelong repair after hair follicle infection. Once the skin has abscess and pustule, the skin will be injured to the dermis layer to remove pus or the pustule will break, leaving scar. There is also a kind of hypertrophic scar, which is a red and swollen appearance left on the spot where acne grows, and more seriously, it will grow slowly due to scratching or external stimulation, and this symptom is often caused by some people with special physique, and this kind of people is too active due to fibroblast in the dermis layer of the skin, and it is over-reacted in the process of wound healing, so that the dermis is not concave but convex after being injured due to inflammation, and becomes hypertrophic skin tissue hyperplasia.
Therefore, it is an urgent technical problem to provide a product which has no toxic and side effects, anti-inflammatory and antibacterial effects, can promote wound healing and relieve excessive skin hyperplasia.
Disclosure of Invention
The invention aims to solve the technical problem of scar hyperplasia caused by medicinal acne and provides a composition containing earthworm protein, which can repair hormone face and prevent scar formation.
Another object of the present invention is to provide a method for preparing the composition containing earthworm protein.
The invention also aims to apply the composition containing the earthworm protein to the preparation of medicines or skin care products for treating the hormonal acne.
The purpose of the invention is realized as follows: a composition comprising earthworm protein, wherein the earthworm protein comprises: the active ingredients in the formula consist of the following components in parts by weight: 10-15 parts of earthworm freeze-dried powder or earthworm dry powder, 5-8 parts of trollius chinensis bunge extract, 5-8 parts of pawpaw extract, 5-8 parts of centella asiatica extract, 3-5 parts of vitamin E and 5-8 parts of humectant.
The humectant is glycerol or trehalose.
The formulation of the composition containing the earthworm protein is emulsion, ointment, cream, gel or aqua, and the formulation consists of the effective components and auxiliary material base of the corresponding formulation.
The formulation of the composition containing the earthworm protein is emulsion, and the formulation comprises the following components in parts by weight: 10-15 parts of earthworm freeze-dried powder, 5-8 parts of trollius chinensis bunge extract, 5-8 parts of pawpaw extract, 5-8 parts of centella asiatica extract, 3-5 parts of vitamin E, 5-8 parts of humectant, 5-8 parts of emulsifier, 0.5-1 part of preservative, 40-68 parts of oil phase matrix and 80-100 parts of purified water.
The emulsifier is one or two of sorbitan and monostearate.
The preservative is one or a combination of two of nipagin and phenoxyethanol.
The oil phase matrix comprises the following components in parts by weight: 12-20 parts of stearic acid, 6-10 parts of glyceryl monostearate, 6-10 parts of No. 15 white oil, 2-5 parts of beeswax, 10-15 parts of liquid paraffin and 4-8 parts of vaseline.
The preparation method of the composition containing the earthworm protein is characterized by comprising the following steps:
(1) preparing an oil phase:
taking the oil phase matrix and vitamin E according to the formula, heating to 75-85 ℃, and uniformly mixing to obtain an oil phase;
(2) preparing an aqueous phase:
adding Lumbricus lyophilized powder or Lumbricus dry powder, flos Trollii extract, fructus Chaenomelis extract, herba Centellae extract, humectant, emulsifier and antiseptic into purified water, heating to 75-85 deg.C, and mixing to obtain water phase;
(3) adding the water phase into the oil phase at 75-85 deg.C, mixing, stirring, and cooling to room temperature to obtain composition emulsion containing Lumbricus protein.
The preparation method of the earthworm freeze-dried powder comprises the following steps: cutting fresh lumbricus into abdomen, removing viscera silt, cleaning with deionized water, cutting into pieces, storing in a refrigerator below-70 deg.C, freezing, crushing the frozen lumbricus, pulverizing with a low temperature pulverizer or under the protection of liquid nitrogen into fine powder with particle size of 70-120 meshes, and freeze drying with a low temperature refrigerator to obtain Lumbricus lyophilized powder.
The application of the composition containing the earthworm protein in preparing medicines or skin care products for treating the hormonal acne is provided.
The invention adopts earthworm protein as a main active component, and extracts of trollius chinensis, pawpaw, centella asiatica and the like are matched to be used as the active components together according to corresponding proportion, so that the earthworm protein can be used for repairing erythroserin acne and has the effect of inhibiting the scar hyperplasia of cystic abscess or polymeric abscess. The earthworm protein composition can prevent pigmentation and scars of the skin of a patient suffering from the hormonal acne in the using process.
Detailed Description
The invention relates to a composition containing earthworm protein, which comprises the following effective components in parts by weight: 10-15 parts of earthworm freeze-dried powder or earthworm dry powder, 5-8 parts of trollius chinensis bunge extract, 5-8 parts of pawpaw extract, 5-8 parts of centella asiatica extract, 3-5 parts of vitamin E and 5-8 parts of humectant.
Preferably, the humectant is glycerin or trehalose. Most preferably, the humectant is trehalose, and trehalose has an anti-inflammatory effect and can inhibit secretion and diffusion of inflammatory factors.
The formula comprises the following components:
lumbricus has effects of clearing heat, arresting convulsion, dredging collaterals, relieving asthma, lowering blood pressure, resisting tumor, dissolving thrombi, reducing blood lipid, and reducing blood viscosity. The Lumbricus has effects of clearing heat, arresting convulsion, dredging collaterals, smoothing extremities, benefiting, dissolving gun, resisting coagulation, and promoting wound healing. The Lumbricus mainly contains enzymes, proteins, polypeptides, lipids, and microelements, and has anticoagulant, antithrombotic, blood pressure lowering, antitumor, antipyretic, antiinflammatory, analgesic, and wound healing promoting effects. The invention adopts freeze-dried earthworm powder or earthworm dry powder. Since the earthworm dry powder has darker color and heavier taste and is difficult to be crushed into fine powder, the earthworm dry powder has granular feel when being used, and is difficult to be uniformly dispersed in a water phase or an oil phase, and the earthworm dry powder is most preferably adopted to optimize the product quality, so that the earthworm protein is not damaged, and the quality requirement of the product can be met. The preparation method of the earthworm freeze-dried powder comprises the following steps: cutting fresh lumbricus into abdomen, removing viscera silt, cleaning with deionized water, cutting into pieces, storing in a refrigerator below-70 deg.C, freezing, crushing the frozen lumbricus, pulverizing with a low temperature pulverizer or under the protection of liquid nitrogen into fine powder with particle size of 70-120 meshes, and freeze drying with a low temperature refrigerator to obtain Lumbricus lyophilized powder.
The globeflower, bitter in taste and slightly cold, enters lung and stomach meridians. The efficacy is as follows: clearing away heat and toxic material. Treating infection, tonsillitis, pharyngitis, acute otitis media, acute myringitis, acute conjunctivitis, acute lymphangitis, aphtha, and furuncle. The trollius chinensis bunge has obvious antibacterial and anti-inflammatory effects and strong antiviral and antioxidant activities. Has various pharmacological effects, including strong antibacterial activity and wide antibacterial spectrum, and especially has strong antibacterial effect on staphylococcus aureus and escherichia coli. Simultaneously has the functions of relieving fever and pain and regulating immunity. The trollius chinensis bunge extract is preferably an aqueous extract of trollius chinensis bunge, and can be extracted by adopting a conventional water extraction process, for example, the medicinal materials are generally decocted and extracted twice, the amount of water used for each time is 6-8 times, the extraction is carried out for 40-80min, and then the extracting solutions are combined and concentrated to 1g crude drug/mL.
Centella asiatica, bitter and pungent, cold, enters liver, spleen and kidney meridians. The efficacy is as follows: clear heat and promote diuresis, remove toxicity and relieve swelling. The centella asiatica extract is white needle crystal extracted from whole plant of centella asiatica of Umbelliferae. Herba Centellae can activate human epithelial cells, promote normal granulation tissue formation, clear heat, promote diuresis, relieve swelling, remove toxic substance, increase local leukocyte of skin, diminish inflammation of skin, and inhibit further deterioration of skin. The asiatic centella extract is preferably an alcohol extract of asiatic centella, and can be extracted by a conventional alcohol extraction process, for example, the medicinal materials are extracted by refluxing with 75% ethanol for 2 times, each time is 8-12 times of the amount of the ethanol, the ethanol is extracted for 40-80min, the filtrate is filtered and combined, the ethanol is recovered from the filtrate under reduced pressure, and the filtrate is concentrated until no alcohol smell exists, which is equivalent to 1g/mL of the crude drug.
Pawpaw, sour and warm, enters liver and spleen meridians. The functional indications are as follows: relaxing tendons and activating collaterals, regulating stomach function and eliminating dampness. The pawpaw has remarkable antibacterial effect and can cure the skin inflammation. The pawpaw extract is preferably an aqueous extract of pawpaw, and can be extracted by adopting the existing pawpaw water reflux extraction process, for example, the medicinal materials are soaked in distilled water and then are heated and refluxed for 2 times, 4-8 times of water is used for refluxing for 40-80min, the heating and refluxing temperature is 85 ℃, then the decoction is filtered, merged, concentrated and freeze-dried, and the concentration is 1g crude drug/mL.
The formulation of the composition containing the earthworm protein can be designed into external formulations such as emulsion, ointment, cream, gel or water agent according to requirements, and the formulation consists of the effective components and auxiliary material matrixes of corresponding formulations.
When the formulation of the composition containing the earthworm protein is emulsion, the formula preferably comprises the following components in parts by weight: 10-15 parts of earthworm freeze-dried powder or earthworm dry powder, 5-8 parts of trollius chinensis bunge extract, 5-8 parts of pawpaw extract, 5-8 parts of centella asiatica extract, 3-5 parts of vitamin E, 5-8 parts of humectant, 5-8 parts of emulsifier, 0.5-1 part of preservative, 40-68 parts of oil phase matrix and 80-100 parts of purified water. Preferably, the emulsifier is one or a combination of sorbitan and monostearate. When the emulsifier is a composition, the components are in any ratio. Preferably, the preservative is one or a combination of two of nipagin and phenoxyethanol. When the preservative is a composition, the components are in any ratio. Preferably, the oil phase matrix comprises the following components in parts by weight: 12-20 parts of stearic acid, 6-10 parts of glyceryl monostearate, 6-10 parts of No. 15 white oil, 2-5 parts of beeswax, 10-15 parts of liquid paraffin and 4-8 parts of vaseline.
The preparation method of the composition containing the earthworm protein comprises the following steps:
(1) preparing an oil phase:
taking the oil phase matrix and vitamin E according to the formula, heating to 75-85 ℃, and mixing uniformly to obtain the oil phase.
(2) Preparing an aqueous phase:
adding Lumbricus lyophilized powder, flos Trollii extract, fructus Chaenomelis extract, herba Centellae extract, humectant, emulsifier and antiseptic into purified water, heating to 75-85 deg.C, and mixing to obtain water phase. The preparation method of the earthworm freeze-dried powder comprises the following steps: cutting fresh Lumbricus open abdomen, removing viscera silt, cleaning with deionized water, cutting, weighing, storing in refrigerator below-70 deg.C, freezing, crushing the frozen Lumbricus, pulverizing with low temperature pulverizer or under protection of liquid nitrogen into fine powder with particle size of 70-120 mesh, and freeze drying with low temperature refrigerator to obtain Lumbricus lyophilized powder.
(3) Adding the water phase into the oil phase at 75-85 deg.C, mixing, stirring, and cooling to room temperature to obtain composition emulsion containing Lumbricus protein.
The application of the composition containing the earthworm protein in preparing medicines or skin care products for treating the hormonal acne is provided. Can be made into lotion, ointment, cream, gel or aqua, etc., and can be used for repairing hormone face to prevent scar formation.
The technical solution of the present invention is further illustrated by the following specific examples, but the present invention is not limited to the specific examples listed below.
In the following examples, the extraction process of the trollius chinensis bunge extract is as follows: decocting and extracting 8 times of purified water for 1 time for 60 min; extracting with 6 times of purified water for 2 times for 60 min; extracting with 4 times of purified water for 30min in the 3 rd time; then combining the extracting solutions and concentrating to 1g crude drug/mL.
The extraction process of the pawpaw extract comprises the following steps: soaking fructus Chaenomelis in distilled water for 1 hr, heating and refluxing for 2 times with 6 times of water each time for 1 hr at 85 deg.C. Filtering, mixing decoctions, concentrating, standing, filtering with gauze, and freeze drying to obtain the final product with concentration of 1g crude drug/mL.
The extraction process of the centella extract comprises the following steps: reflux-extracting herba Centellae with 75% ethanol for 2 times (10 times of the amount of the extract for 1 hr each time), filtering, and mixing filtrates; recovering ethanol from the filtrate under reduced pressure, and concentrating the filtrate until no alcohol smell is obtained, which is equivalent to 1g/mL of crude drug. Purifying with HPD-100 macroporous resin column with maximum sample loading of 12 times volume of sample solution, 50% ethanol as eluent, and 6 times volume of resin column as eluent.
In comparative example 4, trollius chinensis bunge, pawpaw and centella asiatica are commercially available traditional Chinese medicinal materials.
Example 1
The preparation of the composition of this example was carried out according to the following steps:
firstly, preparing earthworm freeze-dried powder:
cutting fresh lumbricus into abdomen, removing viscera silt, cleaning with deionized water, cutting, weighing, storing in a refrigerator at-80 deg.C, freezing, crushing the frozen lumbricus, pulverizing with a low temperature pulverizer or under protection of liquid nitrogen into fine powder with particle size of 70-80 meshes, and freeze drying with a low temperature refrigerator to obtain Lumbricus lyophilized powder;
secondly, preparing an oil phase:
heating 16 parts by weight of stearic acid, 8 parts by weight of glyceryl monostearate, 8 parts by weight of No. 15 white oil, 4 parts by weight of beeswax, 12 parts by weight of liquid paraffin, 6 parts by weight of vaseline and 5 parts by weight of vitamin E to 80 ℃, and uniformly mixing to obtain an oil phase;
thirdly, preparing a water phase:
heating 15 parts by weight of earthworm freeze-dried powder, 8 parts by weight of trollius chinensis bunge extract, 8 parts by weight of pawpaw extract, 8 parts by weight of centella asiatica extract, 8 parts by weight of humectant, 8 parts by weight of emulsifier and 1 part by weight of preservative to 80 ℃, and uniformly mixing to obtain a water phase; the humectant is trehalose; the emulsifier is monostearate; the antiseptic is phenoxyethanol.
Fourthly, adding the water phase into the oil phase at the temperature of 80 ℃, continuously keeping the stirring state after the two phases are mixed, and cooling to the room temperature to obtain the earthworm protein composition emulsion.
Example 2
The preparation of the composition of this example was carried out according to the following steps:
firstly, preparing earthworm dry powder:
cutting fresh lumbricus into abdomen, removing viscera silt, cleaning, cutting into segments, sun-drying (refer to the first part of 2005 edition in Chinese pharmacopoeia), and pulverizing into fine powder with particle size of 90-100 meshes to obtain dry lumbricus powder;
secondly, preparing an oil phase:
heating 16 parts by weight of stearic acid, 8 parts by weight of glyceryl monostearate, 8 parts by weight of No. 15 white oil, 4 parts by weight of beeswax, 12 parts by weight of liquid paraffin, 6 parts by weight of vaseline and 3 parts by weight of vitamin E to 80 ℃, and uniformly mixing to obtain an oil phase;
thirdly, preparing a water phase:
heating 10 parts by weight of earthworm dry powder, 5 parts by weight of trollius chinensis bunge extract, 5 parts by weight of pawpaw extract, 5 parts by weight of centella asiatica extract, 5 parts by weight of humectant, 5 parts by weight of emulsifier and 0.5 part by weight of preservative to 80 ℃ and mixing uniformly to obtain a water phase; wherein the humectant is glycerol, the emulsifier is monostearate, and the antiseptic is phenoxyethanol.
Fourthly, adding the water phase into the oil phase at the temperature of 80 ℃, continuously keeping the stirring state after the two phases are mixed, and cooling to the room temperature to obtain the earthworm protein composition emulsion.
Example 3
The preparation of the composition of this example was carried out according to the following steps:
firstly, preparing earthworm freeze-dried powder:
cutting fresh lumbricus into abdomen, removing viscera silt, cleaning with deionized water, cutting, weighing, storing in a refrigerator at-80 deg.C, freezing, crushing the frozen lumbricus, pulverizing with a low temperature pulverizer or under protection of liquid nitrogen into fine powder with particle size of 70-80 meshes, and freeze drying with a low temperature refrigerator to obtain Lumbricus lyophilized powder;
secondly, preparing an oil phase:
heating 16 parts by weight of stearic acid, 8 parts by weight of glyceryl monostearate, 8 parts by weight of No. 15 white oil, 4 parts by weight of beeswax, 12 parts by weight of liquid paraffin, 6 parts by weight of vaseline and 5 parts by weight of vitamin E to 80 ℃, and uniformly mixing to obtain an oil phase;
thirdly, preparing a water phase:
heating 15 parts by weight of earthworm freeze-dried powder, 8 parts by weight of trollius chinensis bunge extract, 8 parts by weight of pawpaw extract, 8 parts by weight of centella asiatica extract, 8 parts by weight of humectant, 8 parts by weight of emulsifier and 1 part by weight of preservative to 80 ℃, and uniformly mixing to obtain a water phase; wherein the humectant is glycerol, the emulsifier is monostearate, and the antiseptic is phenoxyethanol.
Fourthly, adding the water phase into the oil phase at the temperature of 80 ℃, continuously keeping the stirring state after the two phases are mixed, and cooling to the room temperature to obtain the earthworm protein composition. The humectant is glycerin.
Comparative example 4
The preparation of the comparative example composition was carried out according to the following procedure:
(1) 1 part of carbomer is taken, and 200 parts of distilled water is added for full swelling.
(2) Weighing 12 parts by weight of earthworm freeze-dried powder, 6 parts by weight of trollius chinensis bunge, 6 parts by weight of pawpaw and 6 parts by weight of centella asiatica, adding 20 times of distilled water, and decocting. Then adding 4 parts by weight of vitamin E, 6 parts by weight of humectant, 6 parts by weight of emulsifier and 0.8 part by weight of preservative, uniformly stirring, concentrating into extract, and freeze-drying the extract to form freeze-dried powder. The humectant is glycerin. The emulsifier is monostearate. The preservative is phenoxyethanol.
(3) Adding 8 parts by weight of freeze-dried powder into carbomer swelling solution, adding triethanolamine into the mixed solution, and fixing the volume to obtain gel, thus obtaining the hormone face repairing cream.
100 patients with hormonal facial dermatitis were selected in dermatology department of the academy of traditional Chinese medicine of Heilongjiang province according to the standard of Table 1, wherein 60 patients were grade 3 patients, 20 patients were grade 2 patients, and 20 patients were grade 1 patients.
The earthworm protein composition emulsions prepared in examples 1, 2 and 3 and the hormone face repair cream prepared in comparative example 4 were uniformly applied to the affected parts of the face, respectively, after dividing 60 cases of 3-stage patients into four groups at random, and the grades and identification criteria of the hormone face diseases are shown in table 1, and the days and effects after application of the earthworm protein composition emulsions prepared in examples 1, 2 and 3 and the hormone face repair cream prepared in comparative example 4 are shown in table 2.
The earthworm protein composition emulsion prepared in example 1 was applied uniformly to the affected area of the face for 20 patients of grade 2 and 20 patients of grade 1, and the effect was evaluated after use.
TABLE 1
TABLE 2 (60 patients grade 3)
| 1-10 days | 10-30 days | 30-90 days | 90-180 days | |
| Example 1(15 examples) | Grade 3 | Grade 3 | Level 1 | Level 0 |
| Example 2(15 examples) | Grade 3 | Stage 2 | Stage 2 | Level 1 |
| Example 3(15 examples) | Grade 3 | Stage 2 | Stage 2 | Level 1 |
| COMPARATIVE EXAMPLE 4(15 examples) | Grade 3 | Stage 2 | Grade 3 | Stage 2 |
The earthworm protein composition prepared in example 1, which is a hormone for face dermatitis, had the following effects:
for grade 1, mild hormonal facial skin (20 patients total), used for 1-5 days, the papules became smaller and the skin surface turned red. The number of papules is reduced. There was a slight improvement in inflammation. On day 5-15, most hormone faces will be withdrawn with different degrees, manifested as red, swollen, itchy, and papular. On day 15-90, the skin in withdrawal period shows red, swelling, pimple and exudation symptoms gradually disappear, the appearance of the skin mainly shows dryness and skin fading, the skin gradually approaches to normal, the skin state is unstable, and withdrawal symptoms are easy to appear repeatedly. The appearance and the function of the skin are almost recovered to be normal after days 90-180, and the skin state is greatly improved.
For grade 2, moderate hormone face skin (20 patients in total), erythema, small pimple and red swelling part tend to be reduced after 1-10 days. On day 10-20, most hormone faces will be withdrawn with different degrees, manifested as red, swollen, itchy, and papular. On the 20 th to 90 th days, the skin in the withdrawal period shows red, swollen, pimple and exudation symptoms which are gradually faded, the appearance of the skin mainly shows dry and skin-fading and gradually approaches to normal, the skin state is unstable, and the withdrawal symptoms are easy to appear repeatedly. The appearance and the function of the skin are almost recovered to be normal after days 90-180, and the skin state is greatly improved.
When the earthworm protein composition prepared in example 1 is used for patients with grade 3 hormonal facial dermatitis, the pimple is seriously swollen, the large blister is small, the red swelling part basically disappears, the skin surface is faded, the inflammation gradually disappears, the skin hyperplasia and the bulge do not appear after 1-15 days, and most hormonal faces can be in a withdrawal phenomenon with different weights, blisters and exudation after 15-30 days. On 30-120 days, the skin in the withdrawal period shows red, swelling, pimple and exudation symptoms which gradually disappear, the appearance of the skin mainly shows dryness and skin fading, the skin is gradually close to normal, the skin state is unstable, and the withdrawal symptoms are easy to appear repeatedly. The appearance and the function of the skin are almost normal after 180 days at 120, and the skin state is greatly improved.
As shown in table 2, the patients with grade 3 hormonal facial dermatitis using the earthworm protein composition prepared in example 1 can recover to grade 0 after 90-180 days of use, the skin condition is greatly improved, and the face of the patients is smooth and glossy and is close to the color of normal skin, because the earthworm freeze-dried powder in example 1 mainly contains enzymes, proteins, polypeptides, lipids and trace elements, and has the effects of anticoagulation, antithrombotic, blood pressure reduction, antitumor, antipyretic, anti-inflammatory, analgesic and wound healing acceleration. The trehalose has the functions of diminishing inflammation and inhibiting the secretion and diffusion of inflammatory factors, so that the earthworm protein composition can prevent the skin of a patient suffering from the hormonal acne from generating pigmentation and scars in the process of combining the earthworms and the trehalose.
With the earthworm protein compositions of examples 2, 3, the best condition recovered in patients with hormonal face dermatitis grade 3 can reach grade 1, and after 180 days, the patients' faces have hyperpigmentation or scarring.
With the earthworm protein composition of comparative example 4, the patients with hormonal facial dermatitis grade 3 improved, but the best recovery was achieved only in grade 2, and after 180 days, the patients had hyperpigmentation or scarring in their faces.
Claims (7)
1. A composition comprising earthworm protein, wherein the earthworm protein comprises: the formulation of the composition containing the earthworm protein is emulsion, and the formulation comprises the following components in parts by weight: 10-15 parts of earthworm freeze-dried powder, 5-8 parts of trollius chinensis bunge extract, 5-8 parts of pawpaw extract, 5-8 parts of centella asiatica extract, 3-5 parts of vitamin E, 5-8 parts of humectant, 5-8 parts of emulsifier, 0.5-1 part of preservative, 40-68 parts of oil phase matrix and 80-100 parts of purified water; the humectant is trehalose.
2. The earthworm protein-containing composition of claim 1, wherein: the emulsifier is one or two of sorbitan and monostearate.
3. The earthworm protein-containing composition of claim 1, wherein: the preservative is one or a combination of two of nipagin and phenoxyethanol.
4. The earthworm protein-containing composition of claim 1, wherein: the oil phase matrix comprises the following components in parts by weight: 12-20 parts of stearic acid, 6-10 parts of glyceryl monostearate, 6-10 parts of No. 15 white oil, 2-5 parts of beeswax, 10-15 parts of liquid paraffin and 4-8 parts of vaseline.
5. The process for the preparation of a composition containing earthworm proteins according to any one of claims 1-4, characterised in that it is carried out according to the following steps:
(1) preparing an oil phase:
taking the oil phase matrix and vitamin E according to the formula, heating to 75-85 ℃, and uniformly mixing to obtain an oil phase;
(2) preparing an aqueous phase:
adding Lumbricus lyophilized powder or Lumbricus dry powder, flos Trollii extract, fructus Chaenomelis extract, herba Centellae extract, humectant, emulsifier and antiseptic into purified water, heating to 75-85 deg.C, and mixing to obtain water phase;
(3) adding the water phase into the oil phase at 75-85 deg.C, mixing, stirring, and cooling to room temperature to obtain composition emulsion containing Lumbricus protein.
6. The method of preparing a composition comprising earthworm proteins according to claim 5, wherein: the preparation method of the earthworm freeze-dried powder comprises the following steps: cutting fresh lumbricus into abdomen, removing viscera silt, cleaning with deionized water, cutting into pieces, storing in a refrigerator below-70 deg.C, freezing, crushing the frozen lumbricus, pulverizing with a low temperature pulverizer or under the protection of liquid nitrogen into fine powder with particle size of 70-120 meshes, and freeze drying with a low temperature refrigerator to obtain Lumbricus lyophilized powder.
7. Use of a composition comprising earthworm proteins according to any one of claims 1-4 for the preparation of a medicament or a skin care product for the treatment of hormonal acne.
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