CN104622751A - Traditional Chinese medicine biogel for treating pimples - Google Patents
Traditional Chinese medicine biogel for treating pimples Download PDFInfo
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- CN104622751A CN104622751A CN201510067276.6A CN201510067276A CN104622751A CN 104622751 A CN104622751 A CN 104622751A CN 201510067276 A CN201510067276 A CN 201510067276A CN 104622751 A CN104622751 A CN 104622751A
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Abstract
The invention discloses a traditional Chinese medicine biogel for treating pimples. A preparation method of the traditional Chinese medicine biogel is characterized in that a traditional Chinese medicine extract solution is fermented with bifidobacterium, lactobacillus acidophilus and lactobacillus plantarum to prepare the gel, wherein the traditional Chinese medicine extracts are clove oil, magnolia fragrance flower oil, a radix salviae milliorrhizae extract, a cortex phellodendri extract, a licorice extract and a puerarin extract. The traditional Chinese medicine biogel is significant in curative effect on pimples, free of a side effect, convenient to use, and low in treatment cost, and is directly applied to the skin parts with pimples; and the dosage is obviously lower than that of oral medication.
Description
Technical field
The present invention relates to cosmetics, be specifically related to a kind of Chinese traditional medicine biology gel for the treatment of small pox, belong to cosmetic technical field.
Background technology
Small pox, popular name comedo, acne are a kind of inflammatory disorderses occurring in pilosebaceous unit and surrounding tissue thereof, all kinds of crowd, especially occur comparatively general in youngster, to be apt to occur in the pleomorphism skin lesion such as acne, pimple, pustule, tuberosity of face for feature.To teen-age psychology and social influence very large.
The factors such as the generation of small pox is main and hypersteatosis, pilosebaceous duct block, bacteriological infection and inflammatory reaction are closely related.After adolescing, in human body, the level of androgen particularly testosterone raises rapidly, promotes that sebaceous gland is grown and produces a large amount of sebum.The dyskeratosis of pilosebaceous duct causes catheter blockage, dyssebacia simultaneously, forms horn plug and acne.Multiple-microorganism especially propionibacterium acnes amount reproduction in hair follicle, bacteriogenic lipase decomposes sebum and generates free fatty, simultaneously chemotactic inflammatory cell and medium, finally induces and exacerbate inflammation reaction.
Many Management of Cosmetics Enterprises are proposed product for resolving poxes.In product for resolving poxes, the kind of effective ingredient and content not only determine the quality of anti-acne effect, and also have certain influence to the health of consumer.Antibiotics and glucocorticoids have certain therapeutic effect to comedo and are usually used in medicine for external use.Salicylic acid has medium anti-pox effect and anti-microbial property, and less to the zest of skin, is therefore also commonly used for small pox curative.Compared with medicine, the security requirement of cosmetics is higher, not only requires use safety, and can not produce obvious stimulation and damage to site of administration, more can not produce any Drug resistance.At present, market has a lot of product for resolving poxes abuse of antibiotics, glucocorticoid, chemical drugs phenomenon very serious, result is troubling.
Treatment by Chinese herbs acne, without antibiotic, glucocorticoid, chemical drugs, without security concern, has unique curative effect.Oleum Caryophylli, Magnolia denudata fragrant flower oil, Radix Salviae Miltiorrhizae extract, Cortex Phellodendri extract, Radix Glycyrrhizae extract, Radix Puerariae extract etc. are usually used in Acne treatment, and through repetition test, we find through biofermentation, and its curative effect can improve further.
Summary of the invention
The object of this invention is to provide a kind of not containing the eutherapeutic cosmetics of Acne treatment of antibiotic, glucocorticoid, chemical drugs.
Object of the present invention is achieved through the following technical solutions.
Treat a Chinese traditional medicine biology gel for small pox, it is characterized in that preparation method is: make gel with bacillus bifidus, bacillus acidophilus, Lactobacillus plantarum fermented tcm extract solution; Its Chinese medicine extract is Oleum Caryophylli, Magnolia denudata fragrant flower oil, Radix Salviae Miltiorrhizae extract, Cortex Phellodendri extract, Radix Glycyrrhizae extract, Radix Puerariae extract.
A kind of Chinese traditional medicine biology gel for the treatment of small pox of the present invention, it is characterized in that: Chinese medicine extract solution is Oleum Caryophylli 0.2%, Radix Salviae Miltiorrhizae extract 0.12%, Cortex Phellodendri extract 0.06%, Radix Glycyrrhizae extract 0.06%, Radix Puerariae extract 0.06%, Magnolia denudata fragrant flower oil 0.05% by weight percentage, and all the other are water.
A kind of Chinese traditional medicine biology gel for the treatment of small pox of the present invention, it is characterized in that: culture medium is glucose 0.05%, peptone 0.2%, zinc sulfate 0.0044%, iron sulfate 0.001% by weight percentage, sodium sulfate 0.0002%, magnesium sulfate 0.07%, manganese sulfate 0.0005%, potassium dihydrogen phosphate 0.0001%, dipotassium hydrogen phosphate 0.0001%, all the other are Chinese medicine extract solution.
A kind of Chinese traditional medicine biology gel for the treatment of small pox of the present invention, it is characterized in that preparation method is: culture medium, heat sterilization, when temperature is down to about 39 DEG C, bacillus bifidus, bacillus acidophilus, Lactobacillus plantarum seed liquor are linked in fermentation medium, every strain bacterium seed liquor inoculum concentration is 1.0%, cultivate 48-100h for 38 DEG C, filter, add carbomer 1%, carbamide 3%, allantoin 0.3%, sodium hydroxide 0.2%, ethyl hydroxybenzoate 0.1% after sterilizing by weight percentage, placement is spent the night, and adds water to and makes total amount, stir, obtain gel.
The Chinese traditional medicine biology gel that the present invention treats small pox is external, is directly applied to the skin part producing small pox.
The present invention treats the Chinese traditional medicine biology gel of small pox, and not containing antibiotic, glucocorticoid, chemical drugs, treatment small pox is evident in efficacy, has no side effect.Directly be applied to the skin part of long small pox, easy to use, good medicine is not bitter to the taste, and the compliance of patient is good.Dosage is starkly lower than oral medication, and medical expense is low.
The beneficial effect that the present invention treats small pox is set forth further below by way of pharmacodynamics test and clinical research.Test is below intended to further illustrate effect of the present invention, but not restriction of the present invention.
One, the gel fungistatic effect that prepared by different method for making compares
(1) sample is prepared
1, abiotic fermentation method gel (preservative free ethyl hydroxybenzoate)
Get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g, be heated to sterilizing in 1 hour of boiling, when temperature is down to room temperature, filter, add carbopol (940) 10g, carbamide 30g, allantoin 3g, sodium hydroxide 2g, place and spend the night, add water to 1000g, stir, obtain gel.
2, biological fermentation process gel (preservative free ethyl hydroxybenzoate)
1) get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g; Add glucose 0.5g, peptone 2g, zinc sulfate 0.044g, iron sulfate 0.01g, sodium sulfate 0.002g, magnesium sulfate 0.7g, manganese sulfate 0.005g, potassium dihydrogen phosphate 0.001g, dipotassium hydrogen phosphate 0.001g again.
2) be heated to 1h sterilizing of boiling, when temperature is down to 38 DEG C, be linked in fermentation medium by bacillus bifidus, bacillus acidophilus, Lactobacillus plantarum seed liquor, every strain bacterium seed liquor inoculum concentration is 1g, cultivates 4 days for 38 DEG C;
3) filter, filtrate being heated to is boiled 1h sterilizing, when temperature is down to room temperature, filters, and adds carbopol (940) 10g, carbamide 30g, allantoin 3g, sodium hydroxide 2g, places and spend the night, add water to 1000g, stir, obtain gel.
(2) In Vitro Bacteriostatic of acne pathogenic bacterium is tested
Institute's microbe research room, Shandong Academy of Medical Sciences basis is entrusted to carry out antibacterial experiment in vitro and minimal inhibitory concentration mensuration to staphylococcus aureus and propionibacterium acnes respectively, result shows, gel prepared by biological fermentation process is 26.63mm to the bacteriostatic diameter of staphylococcus aureus, is 25.18 mm to the bacteriostatic diameter of propionibacterium acnes; Gel prepared by abiotic fermentation method is 17.25mm to the bacteriostatic diameter of staphylococcus aureus, is 16.97mm to the bacteriostatic diameter of propionibacterium acnes.The gel prepared of biological fermentation process to the minimal inhibitory concentration of staphylococcus aureus be 3.12mg/mL, the gel prepared of abiotic fermentation method is 9.51mg/mL.More known, the bacteriostatic activity of the standby gel of legal system of the present invention to golden yellow Fructus Vitis viniferae and propionibacterium acnes is obviously better than gel prepared by abiotic fermentation method.
Two, the gel antiinflammatory action that prepared by different method for making compares
The impact of mice caused by dimethylbenzene xylene ear swelling " is got to body weight 24.5 ± 1.8g male mice 30, divided 3 groups often to organize 10 at random by body weight.Be respectively inventive gel group, prepare gel group, blank group by prior art.Test adopts dimethylbenzene swelling method, the each group of even coating in mouse right ear two sides once, wash away ointment with distilled water after 1.5h, dry cotton ball is wiped and is earned, and auris dextra two sides is only evenly coated with dimethylbenzene 0.03ml/, left ear compares, put to death mice after 15min, cut with diameter 8mm card punch ears homalographic, weigh respectively with analytical balance. with the difference of left and right auricle weight for swelling, the average getting each administration group compares with blank group and carries out statistical test, in table 1.
Table 1 affects comparative result (n=10, X ± SD) to mouse dimethylbenzene ear expanding
Group | Swelling (mmg) | Inhibitory rate of intumesce (%) |
Blank group | 21.48±2.15 | |
Biological fermentation process gel | 14.62±2.11* | 31.94 |
Abiotic fermentation method gel | 17.35±2.67* | 19.23 |
Result shows to compare with blank group, and biological fermentation process gel group suppresses dimethylbenzene ear expanding degree to be obviously better than abiotic fermentation method gel group.
Three, different method for making comparitive study
(1) sample is prepared
1, abiotic fermentation method prepares gel
Get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g, be heated to sterilizing in 1 hour of boiling, when temperature is down to room temperature, filters, add carbopol (940) 10g, carbamide 30g, allantoin 3g, sodium hydroxide 2g, ethyl hydroxybenzoate 1g, placement is spent the night, add water to 1000g, stir, obtain gel.
2, biological fermentation process prepares gel
1) get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g; Add glucose 0.5g, peptone 2g, zinc sulfate 0.044g, iron sulfate 0.01g, sodium sulfate 0.002g, magnesium sulfate 0.7g, manganese sulfate 0.005g, potassium dihydrogen phosphate 0.001g, dipotassium hydrogen phosphate 0.001g again.
2) be heated to 1h sterilizing of boiling, when temperature is down to 38 DEG C, be linked in fermentation medium by bacillus bifidus, bacillus acidophilus, Lactobacillus plantarum seed liquor, every strain bacterium seed liquor inoculum concentration is 1g, cultivates 4 days for 38 DEG C;
3) filter, filtrate being heated to is boiled 1h sterilizing, and when temperature is down to room temperature, filter, add carbopol (940) 10g, carbamide 30g, allantoin 3g, sodium hydroxide 2g, ethyl hydroxybenzoate 1g, placement is spent the night, and adds water to 1000g, stirs, and obtains gel.。
(2) different method for making comparitive study
1, data and method: physical data selects patient 61 example of typical small pox skin lesion, wherein man 35 example, female 26 example; Age 15-27 year; Medical history 3 months to 4 years.All patients do not take the medicine for external use of oral medicine and the treatment small pox that may affect small pox treatment in 2 weeks.
2, GP TH: patient is divided into two groups at random, biological fermentation process gel group 31 example, abiotic fermentation method gel group 30 example, give inventive gel, prior art gel respectively, be coated with affected part, every day 2 times one month is the course for the treatment of.All patients at period in a medicine without other medicines.
3, efficacy determination recovery from illness: small pox all disappears; Effective: small pox disappears more than 75%, occasionally there is the pox pox newly gone out individually; Effective: small pox disappears 50%-70%, and the small pox newly gone out is less than 10; Invalid: small pox disappears and is less than 50% or without being clearly better.
4, result: in table 2.
A table 2 liang group therapeutic outcome compares (example, %)
Group | Cure | Effective | Effectively | Invalid | Effectively total |
Fermentation group | 14(45.2) | 12(38.7) | 4(12.9) | 1(3.2) | 30(96.8) |
Non-fermented group | 10(33.3) | 7(23.3) | 6(0.20) | 7(23.3) | 23(76.7) |
Biofermentation gel group total effective rate 100%, abiotic fermentation gel group total effective rate 76.7%, two groups of therapeutic outcomes compare, difference has significance (P<0.05), inventive gel group is obviously better than prior art gel group, and all patients of whole therapeutic process are showed no untoward reaction.
Detailed description of the invention
embodiment 1:
Get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, glucose 0.5g, peptone 2g, zinc sulfate 0.044g, iron sulfate 0.01g, sodium sulfate 0.002g, magnesium sulfate 0.7g, manganese sulfate 0.005g, potassium dihydrogen phosphate 0.001g, dipotassium hydrogen phosphate 0.001g, add water to 1000g, be heated to sterilizing in 1 hour of boiling, when temperature is down to 38 DEG C, bacillus bifidus seed liquid is linked in fermentation medium, inoculum concentration is 10g, cultivate 48 hours for 38 DEG C, filter, be heated to 1h sterilizing of boiling.Add carbopol (940) 10g, Polysorbate (80) 2g, sodium hydroxide 4g, adding distil water, to 1000ml, stirs, and sterilizing after subpackage, obtains gel.
embodiment 2:
Get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g, add glucose 0.5g again, peptone 2g, zinc sulfate 0.044g, iron sulfate 0.01g, sodium sulfate 0.002g, magnesium sulfate 0.7g, manganese sulfate 0.005g, potassium dihydrogen phosphate 0.001g, dipotassium hydrogen phosphate 0.001g, add water to 1000g, heat sterilization, when temperature is down to 37 DEG C, by bacillus bifidus, bacillus acidophilus's seed liquor is linked in fermentation medium, every strain bacterium seed liquor inoculum concentration is 10g, cultivate 72h for 38 DEG C, filter, add PEG(4000) 80g, glycerol 100g, benzalkonium bromide 8g, cross linked sodium polyacrylate 10g, adding distil water is to 1000ml, water milling treatment of colloid is even, with Co 60 radiation sterilization after subpackage, obtain gel.
embodiment 3:
Get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g, add glucose 0.5g again, peptone 2g, zinc sulfate 0.044g, iron sulfate 0.01g, sodium sulfate 0.002g, magnesium sulfate 0.7g, manganese sulfate 0.005g, potassium dihydrogen phosphate 0.001g, dipotassium hydrogen phosphate 0.001g, add water to 1000g, heat sterilization, when temperature is down to 37 DEG C, by bacillus bifidus, bacillus acidophilus, Lactobacillus plantarum seed liquor is linked in fermentation medium, every strain bacterium seed liquor inoculum concentration is 10g, cultivate 72h for 38 DEG C, filter, add PEG(4000) 80g, glycerol 100g, benzalkonium bromide 8g, polyvinylpolypyrrolidone 10g, adding distil water is to 1000ml, water milling treatment of colloid is even, with Co 60 radiation sterilization after subpackage, obtain gel.
embodiment 4:
Get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g, add glucose 0.5g again, peptone 2g, zinc sulfate 0.044g, iron sulfate 0.01g, sodium sulfate 0.002g, magnesium sulfate 0.7g, manganese sulfate 0.005g, potassium dihydrogen phosphate 0.001g, dipotassium hydrogen phosphate 0.001g, add water to 1000g, heat sterilization, when temperature is down to 37 DEG C, by bacillus acidophilus, Lactobacillus plantarum seed liquor is linked in fermentation medium, every strain bacterium seed liquor inoculum concentration is 10g, cultivate 72h for 38 DEG C, filter, add PEG(4000) 80g, glycerol 100g, benzalkonium bromide 8g, cross linked sodium polyacrylate 10g, adding distil water is to 1000ml, water milling treatment of colloid is even, with Co 60 radiation sterilization after subpackage, obtain gel.
embodiment 5:
Get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g, add glucose 0.5g again, peptone 2g, zinc sulfate 0.044g, iron sulfate 0.01g, sodium sulfate 0.002g, magnesium sulfate 0.7g, manganese sulfate 0.005g, potassium dihydrogen phosphate 0.001g, dipotassium hydrogen phosphate 0.001g, add water to 1000g, heat sterilization, when temperature is down to 37 DEG C, 10g bacillus acidophilus seed liquor is linked in fermentation medium, cultivate 72h for 38 DEG C, filter, add PEG(4000) 80g, glycerol 100g, benzalkonium bromide 8g, cross linked sodium polyacrylate 10g, adding distil water is to 1000ml, water milling treatment of colloid is even, with Co 60 radiation sterilization after subpackage, obtain gel.
embodiment 6:
Get Oleum Caryophylli 2g, Radix Salviae Miltiorrhizae extract 1.2g, Cortex Phellodendri extract 0.6g, Radix Glycyrrhizae extract 0.6g, Radix Puerariae extract 0.6g, Magnolia denudata fragrant flower oil 0.5g, add water to 1000g, add glucose 0.5g again, peptone 2g, zinc sulfate 0.044g, iron sulfate 0.01g, sodium sulfate 0.002g, magnesium sulfate 0.7g, manganese sulfate 0.005g, potassium dihydrogen phosphate 0.001g, dipotassium hydrogen phosphate 0.001g, add water to 1000g, heat sterilization, when temperature is down to 37 DEG C, 10g Lactobacillus plantarum seed liquor is linked in fermentation medium, cultivate 72h for 38 DEG C, filter, add PEG(4000) 80g, glycerol 100g, benzalkonium bromide 8g, cross linked sodium polyacrylate 10g, adding distil water is to 1000ml, water milling treatment of colloid is even, with Co 60 radiation sterilization after subpackage, obtain gel.
Claims (4)
1. treat a Chinese traditional medicine biology gel for small pox, it is characterized in that preparation method is: make gel with bacillus bifidus, bacillus acidophilus, Lactobacillus plantarum fermented tcm extract solution; Its Chinese medicine extract is Oleum Caryophylli, Magnolia denudata fragrant flower oil, Radix Salviae Miltiorrhizae extract, Cortex Phellodendri extract, Radix Glycyrrhizae extract, Radix Puerariae extract.
2. a kind of Chinese traditional medicine biology gel for the treatment of small pox according to claim 1, it is characterized in that: Chinese medicine extract solution is Oleum Caryophylli 0.2%, Radix Salviae Miltiorrhizae extract 0.12%, Cortex Phellodendri extract 0.06%, Radix Glycyrrhizae extract 0.06%, Radix Puerariae extract 0.06%, Magnolia denudata fragrant flower oil 0.05% by weight percentage, and all the other are water.
3. a kind of Chinese traditional medicine biology gel for the treatment of small pox according to claim 1, it is characterized in that: culture medium is glucose 0.05%, peptone 0.2%, zinc sulfate 0.0044%, iron sulfate 0.001% by weight percentage, sodium sulfate 0.0002%, magnesium sulfate 0.07%, manganese sulfate 0.0005%, potassium dihydrogen phosphate 0.0001%, dipotassium hydrogen phosphate 0.0001%, all the other are Chinese medicine extract solution.
4. a kind of Chinese traditional medicine biology gel for the treatment of small pox according to claim 1, it is characterized in that preparation method is: culture medium, heat sterilization, when temperature is down to 38 DEG C, bacillus bifidus, bacillus acidophilus, Lactobacillus plantarum seed liquor are linked in fermentation medium, every strain bacterium seed liquor inoculum concentration is 1.0%, cultivate 48-100h for 38 DEG C, filter, add carbomer 1%, carbamide 3%, allantoin 0.3%, sodium hydroxide 0.2%, ethyl hydroxybenzoate 0.1% after sterilizing by weight percentage, placement is spent the night, and adds water to and makes total amount, stir, obtain gel.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106860364A (en) * | 2017-02-24 | 2017-06-20 | 长沙协浩吉生物工程有限公司 | A kind of compound method of ferment anti-acne late frost |
CN108403582A (en) * | 2018-04-08 | 2018-08-17 | 北京皓尔生物科技有限公司 | A kind of anti-acne, acne-removing composition and its application containing antibacterial peptide |
CN111904923A (en) * | 2020-08-11 | 2020-11-10 | 江苏华能药业有限公司 | Probiotic and metazoan composition set |
CN112891294A (en) * | 2021-01-29 | 2021-06-04 | 广州辰美化妆品有限公司 | Acne-removing scar-eliminating repairing skin-care composition and acne-removing cream and emulsion containing same |
CN112972328A (en) * | 2021-02-25 | 2021-06-18 | 云南贝泰妮生物科技集团股份有限公司 | Phellodendron and liquorice acne-removing plant composition and application thereof |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106860364A (en) * | 2017-02-24 | 2017-06-20 | 长沙协浩吉生物工程有限公司 | A kind of compound method of ferment anti-acne late frost |
CN108403582A (en) * | 2018-04-08 | 2018-08-17 | 北京皓尔生物科技有限公司 | A kind of anti-acne, acne-removing composition and its application containing antibacterial peptide |
CN111904923A (en) * | 2020-08-11 | 2020-11-10 | 江苏华能药业有限公司 | Probiotic and metazoan composition set |
CN112891294A (en) * | 2021-01-29 | 2021-06-04 | 广州辰美化妆品有限公司 | Acne-removing scar-eliminating repairing skin-care composition and acne-removing cream and emulsion containing same |
CN112972328A (en) * | 2021-02-25 | 2021-06-18 | 云南贝泰妮生物科技集团股份有限公司 | Phellodendron and liquorice acne-removing plant composition and application thereof |
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