CN105395593A - Itching relieving ointment containing folium artemisiae argyi extract and preparation method thereof - Google Patents
Itching relieving ointment containing folium artemisiae argyi extract and preparation method thereof Download PDFInfo
- Publication number
- CN105395593A CN105395593A CN201510898895.XA CN201510898895A CN105395593A CN 105395593 A CN105395593 A CN 105395593A CN 201510898895 A CN201510898895 A CN 201510898895A CN 105395593 A CN105395593 A CN 105395593A
- Authority
- CN
- China
- Prior art keywords
- folium artemisiae
- artemisiae argyi
- parts
- extract
- ointment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/44—Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
Belonging to the field of medicine, the invention in particular relates to an itching relieving ointment containing a folium artemisiae argyi extract and a preparation method thereof. The itching relieving ointment containing the folium artemisiae argyi extract comprises the following preparation raw materials by weight: 50-60 parts of the folium artemisiae argyi extract, 20-25 parts of horseradish tree leaves, 25-30 parts of Lonicera hypoglauca, 40-60 parts of glycerin, 130-140 parts of liquid paraffin, 110-120 parts of stearic acid, 8-10 parts of triethanolamine, and 500-650 parts of water. The raw materials are prepared by the method provided by the invention into the itching relieving ointment containing the folium artemisiae argyi extract. Being mild and hypoallergenic, the itching relieving ointment can has rapid itching relieving and anti-inflammatory effects, is effective in sterilization and bacteriostasis, and can be used for treatment of skin pruritic diseases of children.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to a kind of antipruritic ointment containing Folium Artemisiae Argyi extract and preparation method thereof.
Background technology
Children's's itching skin disease mainly contains miliaria, dermatitis aestivale, eczema, measles etc., and be usual diseases of childhood, frequently-occurring disease, the numerous children's of serious harm is healthy.At present the treatment of child's itching skin disease is normally adopted to antiallergic chemicals, takes the mode such as Chinese medicine or external washout with traditional Chinese herbs.The toxic and side effects of chemicals is large, and potential danger is more, and is not suitable for child and too much takes; The mode Problems existing taking Chinese medicine or external washout with traditional Chinese herbs is also a lot, and compliance difference taken by such as Chinese medicine, and the inaccurate curative effect that may cause of consumption is slow or untoward reaction is larger; External washout with traditional Chinese herbs complex operation, DeGrain etc.Meanwhile, the skin of child is more tender and lovely, is easily subject to infection and the stimulation of antibacterial, easy allergy, immunity is also lower etc., so be used for the treatment of children's's itching skin disease medicine must hypoallergenic gentle, not only to possess effect that is antipruritic, antiinflammatory, also should possess sterilization, antibacterial effect simultaneously.
Having ointment kind that is antipruritic, anti-inflammatory effect in the market has a lot, but be applicable to the little of child, Chinese patent CN101167811B discloses a kind of external preparation with antiinflammatory, antipruritic, bactericidal action, it is prepared from by Flos Lonicerae, the Fructus Kochiae, the Radix Astragali, Radix Arnebiae (Radix Lithospermi), Cortex Dictamni, Herba Menthae, Borneolum Syntheticum, Periostracum Cicadae, Talcum, Radix Glycyrrhizae and Mel, it can be used as the medicine for the treatment of children's itching skin disease, but these external preparation flavour of a drug are numerous, complicated component is unfavorable for applying.
Therefore, be necessary that research one is applicable to child, energy Quick itch stopping, antiinflammatory, bactericidal, and the antipruritic ointment of pure Chinese medicine had no side effect.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of antipruritic ointment containing Folium Artemisiae Argyi extract and preparation method thereof, should the gentle hypoallergenic of antipruritic ointment containing Folium Artemisiae Argyi extract, child is suitable for, can Quick itch stopping antiinflammatory, effective bactericidal.
To achieve these goals, technical scheme of the present invention is as follows:
An antipruritic ointment containing Folium Artemisiae Argyi extract, comprises the raw materials of following weight portion: Folium Artemisiae Argyi extract 50 ~ 60 parts, leaf of Moringa 20 ~ 25 parts, Flos Lonicerae 25 ~ 30 parts, glycerol 40 ~ 60 parts, liquid paraffin 130 ~ 140 parts, stearic acid 110 ~ 120 parts, triethanolamine 8 ~ 10 parts and 500 ~ 650 parts, water.
As the preferred embodiment of the present invention, the described antipruritic ointment containing Folium Artemisiae Argyi extract comprises the raw materials of following weight portion: Folium Artemisiae Argyi extract 58 parts, leaf of Moringa 22 parts, Flos Lonicerae 26 parts, glycerol 50 parts, liquid paraffin 135 parts, stearic acid 114 parts, triethanolamine 9 parts and 586 parts, water.
Further, described Folium Artemisiae Argyi extract is made up of Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract;
The preparation method of described Folium Artemisiae Argyi volatile oil comprises the steps: to get fresh Folium Artemisiae Argyi, rubbing is placed in supercritical carbon dioxide extraction apparatus, regulation and control carbon dioxide flow is 16 ~ 20L/h, extracting pressure is 15 ~ 20MPa, extraction temperature is 30 ~ 35 DEG C, extraction time is 1.5 ~ 2h, and decompression separation obtains Folium Artemisiae Argyi volatile oil, retains Folium Artemisiae Argyi residue;
The preparation method of described Folium Artemisiae Argyi ethanol extract comprises the steps: to get described Folium Artemisiae Argyi residue, the volume fraction adding residue weight 4 ~ 6 times is the alcoholic solution of 60 ~ 70%, extract 2 ~ 3 times at 60 ~ 70 DEG C, each extraction 2 ~ 3h, filter merge extractive liquid, being evaporated to relative density at 60 DEG C is the extractum of 1.25 ~ 1.30, obtained Folium Artemisiae Argyi ethanol extract.
Further, the weight ratio of described Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract is (0.2 ~ 0.6): 1; Be preferably 0.4:1.
The preparation method of the above-mentioned antipruritic ointment containing Folium Artemisiae Argyi extract, comprises the following steps:
S1, get fresh leaf of Moringa, rub, the volume fraction adding its weight 6 ~ 8 times is the alcoholic solution of 70 ~ 80%, microwave extraction 15 ~ 25min, microwave power is 600 ~ 800w, and Extracting temperature is 45 ~ 55 DEG C, filters, it is the extractum of 1.25 ~ 1.30 that extracting solution is evaporated to relative density at 60 DEG C, obtained moringa oleifera leaf extractive;
S2, get fresh Flos Lonicerae, rub, the volume fraction adding its weight 5 ~ 7 times is the alcoholic solution of 60 ~ 70%, microwave extraction 15 ~ 25min, microwave power is 600 ~ 800w, and Extracting temperature is 45 ~ 55 DEG C, filters, it is the extractum of 1.25 ~ 1.30 that extracting solution is evaporated to relative density at 60 DEG C, obtained Lonicera confusa extract;
S3, get glycerol, liquid paraffin, stearic acid, triethanolamine and water and be placed in container, heating for dissolving also stirs, and then natural condensation to 40 ~ 45 DEG C add Folium Artemisiae Argyi extract, moringa oleifera leaf extractive and Lonicera confusa extract, stir, to obtain final product.
The antipruritic ointment that the present invention contains Folium Artemisiae Argyi extract contains Folium Artemisiae Argyi extract, moringa oleifera leaf extractive and Lonicera confusa extract three kinds of natural plant active components.Wherein, Folium Artemisiae Argyi extract contains the Multiple components such as volatile oil, flavonoid, Folium eucalypti globueli (Eucalyptus globulus Labill.) alkanes, triterpenes, has antibacterial, the multiple efficacies such as antiviral, antiallergic.Leaf of Moringa is rich in several mineral materials, vitamin, aminoacid, antioxygen element and naturally anti-scorching body, has the effects such as blood sugar lowering, blood fat reducing, blood pressure lowering, antitumor, antioxidation, relieving constipation, diuresis, anthelmintic, improving water flood.Flos Lonicerae contains the Multiple components such as chlorogenic acid, volatile oil, flavonoid, triterpenoid saponin, has the effects such as antibacterial, antiinflammatory.Above component and glycerol, liquid paraffin, stearic acid, triethanolamine and water combine the gentle hypoallergenic of antipruritic ointment made the present invention and contain Folium Artemisiae Argyi extract, and child is suitable for, can Quick itch stopping antiinflammatory, effective bactericidal.Clinical trial proves, the antipruritic ointment that the present invention contains Folium Artemisiae Argyi extract can be used as the external used medicine for the treatment of children's itching skin disease.
Therefore, compared with prior art, advantage of the present invention is:
(1) the invention provides a kind of antipruritic ointment containing Folium Artemisiae Argyi extract newly, it contains the multiple natural plant active components such as Folium Artemisiae Argyi volatile oil, Folium Artemisiae Argyi ethanol extract, moringa oleifera leaf extractive and Lonicera confusa extract, is a kind of pure natural plant preparation, effective bactericidal, also there is the effects such as antipruritic, antiinflammatory, lasting medicine is stablized, gentle hypoallergenic, can be used for treatment children's itching skin disease, evident in efficacy, have no side effect, and formula of the present invention is simple, cost of material is low.
(2) preparation that the present invention contains the antipruritic ointment of Folium Artemisiae Argyi extract utilizes the volatile oil of supercritical carbon dioxide extraction Folium Artemisiae Argyi, and recycling Folium Artemisiae Argyi residue carries out alcohol extraction, obtains Folium Artemisiae Argyi ethanol extract; Utilize the active component of microwave extraction leaf of Moringa and Flos Lonicerae; Preparation technology of the present invention is fully extracted the effective ingredient in raw material, and maximum using raw material is worth, and stable preparation process, condition is controlled, can be applicable.
Detailed description of the invention
Further describe the present invention below by way of detailed description of the invention, but the present invention is not limited only to following examples.Within the scope of the invention or not departing from content of the present invention, spirit and scope, antipruritic ointment containing Folium Artemisiae Argyi extract of the present invention is suitably improved, replaces the identical component of effect, will become apparent to those skilled in the art that they are all deemed to be included within scope of the present invention.
embodiment 1, the present invention contain antipruritic ointment and the preparation thereof of Folium Artemisiae Argyi extract
The antipruritic ointment that the present embodiment contains Folium Artemisiae Argyi extract is prepared from by the raw material of following weight portion: Folium Artemisiae Argyi extract 58 parts, leaf of Moringa 22 parts, Flos Lonicerae 26 parts, glycerol 50 parts, liquid paraffin 135 parts, stearic acid 114 parts, triethanolamine 9 parts and 586 parts, water
Described Folium Artemisiae Argyi extract is made up of Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract, and the weight ratio of described Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract is 0.4:1;
The preparation method of described Folium Artemisiae Argyi volatile oil comprises the steps: to get fresh Folium Artemisiae Argyi, rubbing is placed in supercritical carbon dioxide extraction apparatus, regulation and control carbon dioxide flow is 18L/h, extracting pressure is 20MPa, extraction temperature is 32 DEG C, extraction time is 1.5h, and decompression separation obtains Folium Artemisiae Argyi volatile oil, retains Folium Artemisiae Argyi residue;
The preparation method of described Folium Artemisiae Argyi ethanol extract comprises the steps: to get described Folium Artemisiae Argyi residue, the volume fraction adding residue weight 5 times is the alcoholic solution of 65%, extract 3 times at 65 DEG C, each extraction 2h, filter merge extractive liquid, being evaporated to relative density at 60 DEG C is the extractum of 1.25, obtained Folium Artemisiae Argyi ethanol extract.
The present embodiment contains the preparation method of the antipruritic ointment of Folium Artemisiae Argyi extract:
S1, get fresh leaf of Moringa, rub, the volume fraction adding its weight 7 times is the alcoholic solution of 75%, microwave extraction 20min, microwave power is 800w, and Extracting temperature is 50 DEG C, filter, it is the extractum of 1.25 that extracting solution is evaporated to relative density at 60 DEG C, obtained moringa oleifera leaf extractive;
S2, get fresh Flos Lonicerae, rub, the volume fraction adding its weight 6 times is the alcoholic solution of 65%, microwave extraction 20min, microwave power is 800w, and Extracting temperature is 50 DEG C, filter, it is the extractum of 1.25 that extracting solution is evaporated to relative density at 60 DEG C, obtained Lonicera confusa extract;
S3, get glycerol, liquid paraffin, stearic acid, triethanolamine and water and be placed in container, heating for dissolving also stirs, and then natural condensation to 40 DEG C adds Folium Artemisiae Argyi extract, moringa oleifera leaf extractive and Lonicera confusa extract, stirs, to obtain final product.
embodiment 2, the present invention contain antipruritic ointment and the preparation thereof of Folium Artemisiae Argyi extract
The antipruritic ointment that the present embodiment contains Folium Artemisiae Argyi extract is prepared from by the raw material of following weight portion: Folium Artemisiae Argyi extract 50 parts, leaf of Moringa 25 parts, Flos Lonicerae 25 parts, glycerol 40 parts, liquid paraffin 140 parts, stearic acid 110 parts, triethanolamine 10 parts and 600 parts, water.
Described Folium Artemisiae Argyi extract is made up of Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract, and the weight ratio of described Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract is 0.2:1;
The preparation method of described Folium Artemisiae Argyi volatile oil comprises the steps: to get fresh Folium Artemisiae Argyi, rubbing is placed in supercritical carbon dioxide extraction apparatus, regulation and control carbon dioxide flow is 16L/h, extracting pressure is 20MPa, extraction temperature is 35 DEG C, extraction time is 2h, and decompression separation obtains Folium Artemisiae Argyi volatile oil, retains Folium Artemisiae Argyi residue;
The preparation method of described Folium Artemisiae Argyi ethanol extract comprises the steps: to get described Folium Artemisiae Argyi residue, the volume fraction adding residue weight 4 times is the alcoholic solution of 70%, extract 2 times at 60 DEG C, each extraction 3h, filter merge extractive liquid, being evaporated to relative density at 60 DEG C is the extractum of 1.30, obtained Folium Artemisiae Argyi ethanol extract.
The present embodiment contains the preparation method of the antipruritic ointment of Folium Artemisiae Argyi extract:
S1, get fresh leaf of Moringa, rub, the volume fraction adding its weight 6 times is the alcoholic solution of 80%, microwave extraction 15min, microwave power is 600w, and Extracting temperature is 45 DEG C, filter, it is the extractum of 1.30 that extracting solution is evaporated to relative density at 60 DEG C, obtained moringa oleifera leaf extractive;
S2, get fresh Flos Lonicerae, rub, the volume fraction adding its weight 5 times is the alcoholic solution of 70%, microwave extraction 15min, microwave power is 800w, and Extracting temperature is 55 DEG C, filter, it is the extractum of 1.30 that extracting solution is evaporated to relative density at 60 DEG C, obtained Lonicera confusa extract;
S3, get glycerol, liquid paraffin, stearic acid, triethanolamine and water and be placed in container, heating for dissolving also stirs, and then natural condensation to 45 DEG C adds Folium Artemisiae Argyi extract, moringa oleifera leaf extractive and Lonicera confusa extract, stirs, to obtain final product.
embodiment 3, the present invention contain antipruritic ointment and the preparation thereof of Folium Artemisiae Argyi extract
The antipruritic ointment that the present embodiment contains Folium Artemisiae Argyi extract is prepared from by the raw material of following weight portion: Folium Artemisiae Argyi extract 60 parts, leaf of Moringa 20 parts, Flos Lonicerae 30 parts, glycerol 60 parts, liquid paraffin 130 parts, stearic acid 120 parts, triethanolamine 8 parts and 572 parts, water.
Described Folium Artemisiae Argyi extract is made up of Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract, and the weight ratio of described Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract is 0.6:1;
The preparation method of described Folium Artemisiae Argyi volatile oil comprises the steps: to get fresh Folium Artemisiae Argyi, rubbing is placed in supercritical carbon dioxide extraction apparatus, regulation and control carbon dioxide flow is 20L/h, extracting pressure is 15MPa, extraction temperature is 30 DEG C, extraction time is 1.5h, and decompression separation obtains Folium Artemisiae Argyi volatile oil, retains Folium Artemisiae Argyi residue;
The preparation method of described Folium Artemisiae Argyi ethanol extract comprises the steps: to get described Folium Artemisiae Argyi residue, the volume fraction adding residue weight 6 times is the alcoholic solution of 60%, extract 3 times at 70 DEG C, each extraction 2h, filter merge extractive liquid, being evaporated to relative density at 60 DEG C is the extractum of 1.25, obtained Folium Artemisiae Argyi ethanol extract.
The present embodiment contains the preparation method of the antipruritic ointment of Folium Artemisiae Argyi extract:
S1, get fresh leaf of Moringa, rub, the volume fraction adding its weight 7 times is the alcoholic solution of 70%, microwave extraction 25min, microwave power is 800w, and Extracting temperature is 55 DEG C, filter, it is the extractum of 1.25 that extracting solution is evaporated to relative density at 60 DEG C, obtained moringa oleifera leaf extractive;
S2, get fresh Flos Lonicerae, rub, the volume fraction adding its weight 7 times is the alcoholic solution of 60%, microwave extraction 25min, microwave power is 600w, and Extracting temperature is 45 DEG C, filter, it is the extractum of 1.25 that extracting solution is evaporated to relative density at 60 DEG C, obtained Lonicera confusa extract;
S3, get glycerol, liquid paraffin, stearic acid, triethanolamine and water and be placed in container, heating for dissolving also stirs, and then natural condensation to 40 DEG C adds Folium Artemisiae Argyi extract, moringa oleifera leaf extractive and Lonicera confusa extract, stirs, to obtain final product.
comparative example one
This comparative example is as different from Example 1: described Folium Artemisiae Argyi extract is Folium Artemisiae Argyi ethanol extract, its preparation method: get fresh Folium Artemisiae Argyi and rub, add Folium Artemisiae Argyi weight 5 times, volume fraction is the alcoholic solution of 65%, extract 3 times at 65 DEG C, each extraction 2h, filter merge extractive liquid, being evaporated to relative density at 60 DEG C is the extractum of 1.25, obtained Folium Artemisiae Argyi extract.
Other are identical with embodiment 1.
comparative example two
The antipruritic ointment that this comparative example contains Folium Artemisiae Argyi extract is prepared from by the raw material of following weight portion: Folium Artemisiae Argyi extract 58 parts, Flos Lonicerae 26 parts, glycerol 50 parts, liquid paraffin 135 parts, stearic acid 114 parts, triethanolamine 9 parts and 586 parts, water
Described Folium Artemisiae Argyi extract is made up of Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract, and the weight ratio of described Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract is 0.4:1;
The preparation method of described Folium Artemisiae Argyi volatile oil comprises the steps: to get fresh Folium Artemisiae Argyi, rubbing is placed in supercritical carbon dioxide extraction apparatus, regulation and control carbon dioxide flow is 18L/h, extracting pressure is 20MPa, extraction temperature is 32 DEG C, extraction time is 1.5h, and decompression separation obtains Folium Artemisiae Argyi volatile oil, retains Folium Artemisiae Argyi residue;
The preparation method of described Folium Artemisiae Argyi ethanol extract comprises the steps: to get described Folium Artemisiae Argyi residue, the volume fraction adding residue weight 5 times is the alcoholic solution of 65%, extract 3 times at 65 DEG C, each extraction 2h, filter merge extractive liquid, being evaporated to relative density at 60 DEG C is the extractum of 1.25, obtained Folium Artemisiae Argyi ethanol extract.
The present embodiment contains the preparation method of the antipruritic ointment of Folium Artemisiae Argyi extract:
S1, get fresh Flos Lonicerae, rub, the volume fraction adding its weight 6 times is the alcoholic solution of 65%, microwave extraction 20min, microwave power is 800w, and Extracting temperature is 50 DEG C, filter, it is the extractum of 1.25 that extracting solution is evaporated to relative density at 60 DEG C, obtained Lonicera confusa extract;
S2, get glycerol, liquid paraffin, stearic acid, triethanolamine and water and be placed in container, heating for dissolving also stirs, and then natural condensation to 40 DEG C adds Folium Artemisiae Argyi extract and Lonicera confusa extract, stirs, to obtain final product.
test example one, the present invention contain the antiinflammatory action of the antipruritic ointment of Folium Artemisiae Argyi extract
One, materials and methods
1, animal: one-level ICR mice 60, body weight 20 ~ 22g, male and female half and half.
2, medicine: make ointment according to the raw material of the embodiment of the present invention 1 ~ 3, comparative example one, two and method.
3, animal grouping: animal is divided into 6 groups at random, often organize 10:
Matched group: ointment base material 2.0gkg-1;
1 group, ointment: the ointment 2.0gkg that embodiment 1 is obtained
-1;
2 groups, ointment: the ointment 2.0gkg that embodiment 2 is obtained
-1;
3 groups, ointment: the ointment 2.0gkg that embodiment 3 is obtained
-1;
4 groups, ointment: the ointment 2.0gkg that comparative example one is obtained
-1;
5 groups, ointment: the ointment 2.0gkg that comparative example two is obtained
-1.
4, experimental technique: experimental day, by each group of dosage in animal auris dextra (the positive and negative both sides of auricle) coating, 60min after medication, medicine is cleaned with dry cotton ball, drip in auris dextra (the positive and negative both sides of auricle) and be coated with xylol, only, coating is once more simultaneously for 30ul/, after 15min, de-neck puts to death animal, cut two ears, with diameter 0.8cm card punch, two ears are broken into disk, weigh, it is swelling that auris dextra subtracts left ear weight difference, the difference between comparative drug group and matched group.The results are shown in Table 1.
Two, result
Table 1 xylol causes the impact (X ± SD) of Mice Auricle inflammation
Compared with matched group, * P < 0.05, * * P < 0.01.
From upper table 1, the antipruritic ointment that the present invention contains Folium Artemisiae Argyi extract has the effect suppressing xylol to cause Mice Auricle inflammation, has obvious antiinflammatory action.Compared with matched group, 1 group, ointment has pole significant difference (P < 0.01), and 2 groups, ointment and 3 groups, ointment have significant difference (P < 0.05).
test example two, the present invention contain the itching-relieving action of the antipruritic ointment of Folium Artemisiae Argyi extract
One, materials and methods
1, animal: Cavia porcellus 60, body weight 250 ~ 300g, male and female half and half.
2, medicine: make ointment according to the raw material of the embodiment of the present invention 1 ~ 3, comparative example one, two and method.
3, animal grouping: animal is divided into 6 groups at random, often organize 10:
Matched group: ointment base material 2.0gkg-1;
1 group, ointment: the ointment 2.0gkg that embodiment 1 is obtained
-1;
2 groups, ointment: the ointment 2.0gkg that embodiment 2 is obtained
-1;
3 groups, ointment: the ointment 2.0gkg that embodiment 3 is obtained
-1;
4 groups, ointment: the ointment 2.0gkg that comparative example one is obtained
-1;
5 groups, ointment: the ointment 2.0gkg that comparative example two is obtained
-1.
4, experimental technique: 24h before experiment, shaves hair to each group of right back instep of Cavia porcellus, shaves mao coating 1 time by this group dosage in the right back instep of animal.Test the same day, abrade right back foot with coarse sandpaper and shave hair place, area 1cm
2, local repastes medicine 1 time, starts at wound surface Chu Di 0.01% histamine phosphate 0.05ml/ only, after this to use 0.02%, 0.03%, 0.04% progressive concentration coating successively every 3min after 10min, is only 0.05ml/ at every turn.Until Cavia porcellus later add right back sufficient time histamine phosphate's total amount (μ g) of giving be itch-threshold, record the itch-threshold of relatively more each group.The results are shown in Table 2.
Two, result
Table 2 causes the sufficient impact (X ± SD) of itching of Cavia porcellus to histamine phosphate
Compared with matched group, * P < 0.05, * * P < 0.01.
From upper table 1, the antipruritic ointment that the present invention contains Folium Artemisiae Argyi extract has good itching-relieving action.Compared with matched group, 1 group, ointment has pole significant difference (P < 0.01), and 2 groups, ointment and 3 groups, ointment have significant difference (P < 0.05).
To sum up test example one and test example two, the antipruritic ointment that the present invention contains Folium Artemisiae Argyi extract has good antiinflammatory, itching-relieving action, and wherein with the best results of embodiment 1, therefore embodiment 1 is most preferred embodiment of the present invention.Comparative example one is not containing Folium Artemisiae Argyi volatile oil of the present invention, and comparative example two is not containing moringa oleifera leaf extractive, and its antiinflammatory, antipruritic effect significantly weaken, and illustrates between each plant extract of the present invention collaborative, brings excellent antiinflammatory, itching-relieving efficacies.
test example three, the present invention contain the vitro antibacterial activity of the antipruritic ointment of Folium Artemisiae Argyi extract
Test liquid: the ointment 40g of Example 1, adds 0.9% aseptic sodium chloride solution 100ml.Be diluted to 1:10 test liquid, for subsequent use.
Measure minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC): measure MIC with Double broth dilution method.In the serial agar plate surface dibbling antibacterial containing test liquid, bacterium liquid ultimate density is 105CFU/ml, 18h is cultivated in aerobe 37 DEG C, fungus 27 DEG C cultivates 24h, anaerobe is put anaerobism incubator 37 DEG C and is hatched 48h, the interior contained least concentration for reagent of plate having no bacterial growth is its minimal inhibitory concentration (MIC, mg/L).With staphylococcus aureus in experiment, colon bacillus makes Quality-control strains.MIC is surveyed with meat soup doubling dilution, get again and have no bacterial growth pipe culture fluid 0.1ml in without medicine agar plate surface, push away with sterile glass rod even, cultivate (condition measures with MIC), lowest concentration of drug still without bacterial growth is its minimum bactericidal concentration (MBC), the results are shown in Table 3.
Table 3 antibacterial experiment result
| Penicillium | Candida albicans | Staphylococcus aureus | Staphylococcus epidermidis | Escherichia coli | Bacillus subtilis | |
| MIC | 1000 | 500 | 125 | 125 | 1000 | 125 |
| MBC | 2000 | 1000 | 1000 | 500 | 1000 | 500 |
From upper table 3, the antipruritic ointment that the present invention contains Folium Artemisiae Argyi extract has significantly antibacterial or bactericidal action to 6 test strains such as testing staphylococcus aureus used.
test example four, the present invention contain the clinical trial of the antipruritic ointment of Folium Artemisiae Argyi extract
(1) study subject is selected
Western medicine diagnostic criteria:
Papular urticaria: be more common in child and teenager, summer and autumn of being everlasting occurs, can arthropod bite history; Being apt to occur in distal limbs and waist buttocks, is many with the lower part of the body especially; Skin lesion is welt sample spindle pimple, and can there be minor carbuncle in central authorities, conscious pruritus; Skin lesion disappeared at 7 ~ 10 days, but new skin lesion constantly occurs.
(2) clinical trial protocol
1, physical data
Choose in January, 2013 ~ 2014 year January in medical patient's totally 80 examples of Dermatology Outpatient Department.Wherein male 50 example, women 30 example, 1 ~ 6 years old age.This group MethodsThe cases enrolled standard:
2, test method
Use the antipruritic ointment containing Folium Artemisiae Argyi extract prepared by the embodiment of the present invention 1, bathing every day embrocates affected part 2 times.6 days courses for the treatment of.7th heaven-made Outcome measure.
(1) a pruritus point level Four scores (see new Chinese medicine guideline of clinical investigations standard)
0 without any degree pruritus;
1 is slight: slightly itch, and can not grab, and strokes and pat and can alleviate, without the secondary skin lesion of scratching
2 moderates: obviously pruritus, can't help grabbing, the slight secondary skin lesion caused of scratching as seen
3 severes: play is itched, emotion is uneasy, impact sleep and work, the obvious secondary skin lesion caused of locally scratching as seen.
(2) a skin lesion point level Four scores (see new Chinese medicine guideline of clinical investigations standard)
0 without skin lesion, or only has pigmentation;
1 is slight, and erythra number is within 5;
2 moderates, erythra number is between 5-15;
3 severes, erythra number is more than 16.
3, curative effect determinate standard
Recovery from illness: gargalesthesia disappears, and skin lesion disappears completely or only has pigmentation, and namely score value is 0, and therapeutic index is 100%.
Effective: gargalesthesia obviously alleviates, skin lesion disappears more than 60%, improves more than two grades, therapeutic index >=60% < 100% at once before proportion by subtraction treatment.
Effective: gargalesthesia alleviates to some extent, skin lesion disappears more than 30%, but is not 60%, obtains before proportion by subtraction is treated and improves more than a grade, below two grades, and therapeutic index >=30% < 60%.
Invalid: gargalesthesia bone is front or more very, skin lesion disappears not as good as 30% or increases and increases the weight of.
4, therapeutic outcome:
Children's's papular urticaria patient 80 example is treated in this clinical trial altogether, wherein obvious effective rate 87.50%, total effective rate 93.75%.The cure rate of antipruritic ointment to children's's papular urticaria that proof the present invention contains Folium Artemisiae Argyi extract is high, short treating period, and therapeutic effect is remarkable, expands and is applied to children's's itching skin disease such as miliaria, dermatitis aestivale, eczema, also obtain good curative effect in test.Therefore, can use as the external used medicine of clinical treatment children's itching skin disease by the present invention's antipruritic ointment of containing Folium Artemisiae Argyi extract, clinical generalization value is high.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred implementation should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. the antipruritic ointment containing Folium Artemisiae Argyi extract, it is characterized in that, comprise the raw materials of following weight portion: Folium Artemisiae Argyi extract 50 ~ 60 parts, leaf of Moringa 20 ~ 25 parts, Flos Lonicerae 25 ~ 30 parts, glycerol 40 ~ 60 parts, liquid paraffin 130 ~ 140 parts, stearic acid 110 ~ 120 parts, triethanolamine 8 ~ 10 parts and 500 ~ 650 parts, water.
2. the antipruritic ointment as claimed in claim 1 containing Folium Artemisiae Argyi extract, it is characterized in that, the described antipruritic ointment containing Folium Artemisiae Argyi extract comprises the raw materials of following weight portion: Folium Artemisiae Argyi extract 58 parts, leaf of Moringa 22 parts, Flos Lonicerae 26 parts, glycerol 50 parts, liquid paraffin 135 parts, stearic acid 114 parts, triethanolamine 9 parts and 586 parts, water.
3. the antipruritic ointment as claimed in claim 1 or 2 containing Folium Artemisiae Argyi extract, it is characterized in that, described Folium Artemisiae Argyi extract is made up of Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract;
The preparation method of described Folium Artemisiae Argyi volatile oil comprises the steps: to get fresh Folium Artemisiae Argyi, rubbing is placed in supercritical carbon dioxide extraction apparatus, regulation and control carbon dioxide flow is 16 ~ 20L/h, extracting pressure is 15 ~ 20MPa, extraction temperature is 30 ~ 35 DEG C, extraction time is 1.5 ~ 2h, and decompression separation obtains Folium Artemisiae Argyi volatile oil, retains Folium Artemisiae Argyi residue;
The preparation method of described Folium Artemisiae Argyi ethanol extract comprises the steps: to get described Folium Artemisiae Argyi residue, the volume fraction adding residue weight 4 ~ 6 times is the alcoholic solution of 60 ~ 70%, extract 2 ~ 3 times at 60 ~ 70 DEG C, each extraction 2 ~ 3h, filter merge extractive liquid, being evaporated to relative density at 60 DEG C is the extractum of 1.25 ~ 1.30, obtained Folium Artemisiae Argyi ethanol extract.
4. the antipruritic ointment as claimed in claim 3 containing Folium Artemisiae Argyi extract, it is characterized in that, the weight ratio of described Folium Artemisiae Argyi volatile oil and Folium Artemisiae Argyi ethanol extract is (0.2 ~ 0.6): 1.
5. prepare a method for the antipruritic ointment as claimed in claim 1 or 2 containing Folium Artemisiae Argyi extract, it is characterized in that, comprise the following steps:
S1, get fresh leaf of Moringa, rub, the volume fraction adding its weight 6 ~ 8 times is the alcoholic solution of 70 ~ 80%, microwave extraction 15 ~ 25min, microwave power is 600 ~ 800w, and Extracting temperature is 45 ~ 55 DEG C, filters, it is the extractum of 1.25 ~ 1.30 that extracting solution is evaporated to relative density at 60 DEG C, obtained moringa oleifera leaf extractive;
S2, get fresh Flos Lonicerae, rub, the volume fraction adding its weight 5 ~ 7 times is the alcoholic solution of 60 ~ 70%, microwave extraction 15 ~ 25min, microwave power is 600 ~ 800w, and Extracting temperature is 45 ~ 55 DEG C, filters, it is the extractum of 1.25 ~ 1.30 that extracting solution is evaporated to relative density at 60 DEG C, obtained Lonicera confusa extract;
S3, get glycerol, liquid paraffin, stearic acid, triethanolamine and water and be placed in container, heating for dissolving also stirs, and then natural condensation to 40 ~ 45 DEG C add Folium Artemisiae Argyi extract, moringa oleifera leaf extractive and Lonicera confusa extract, stir, to obtain final product.
6. the purposes of the antipruritic ointment as claimed in claim 1 or 2 containing Folium Artemisiae Argyi extract in the medicine of preparation treatment children's itching skin disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510898895.XA CN105395593A (en) | 2015-12-09 | 2015-12-09 | Itching relieving ointment containing folium artemisiae argyi extract and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510898895.XA CN105395593A (en) | 2015-12-09 | 2015-12-09 | Itching relieving ointment containing folium artemisiae argyi extract and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105395593A true CN105395593A (en) | 2016-03-16 |
Family
ID=55461590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510898895.XA Pending CN105395593A (en) | 2015-12-09 | 2015-12-09 | Itching relieving ointment containing folium artemisiae argyi extract and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105395593A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107536756A (en) * | 2017-09-07 | 2018-01-05 | 平舆玛雅生物工程有限公司 | One kind is dispelled miliaria cold cream and preparation method thereof |
| CN109363969A (en) * | 2018-10-08 | 2019-02-22 | 广东暨晴生物医药科技有限公司 | A kind of method of comprehensive utilization of wormwood effective component |
| CN111956558A (en) * | 2020-09-17 | 2020-11-20 | 两面针（江苏）实业有限公司 | Artemisia argyi extract and preparation method and application thereof |
| CN115944662A (en) * | 2022-12-23 | 2023-04-11 | 中南民族大学 | Application of folium artemisiae argyi volatile oil serving as TRPA1 pathway inhibitor in anti-inflammatory and antipruritic medicines |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485622A (en) * | 2009-03-03 | 2009-07-22 | 张嘉恒 | Shampoo containing extract of Moringa oleifera and preparation method thereof |
| CN102813660A (en) * | 2012-09-13 | 2012-12-12 | 前郭尔罗斯蒙古族自治县医院 | External medicine for treating skin eczema |
| EP2727473A2 (en) * | 2011-06-28 | 2014-05-07 | Vivozon, Inc. | Combination of effective substances causing synergistic effects of multiple targeting and use thereof |
| CN104474008A (en) * | 2014-12-10 | 2015-04-01 | 遵义医学院 | Honeysuckle prickly heat water and preparation method thereof |
-
2015
- 2015-12-09 CN CN201510898895.XA patent/CN105395593A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485622A (en) * | 2009-03-03 | 2009-07-22 | 张嘉恒 | Shampoo containing extract of Moringa oleifera and preparation method thereof |
| EP2727473A2 (en) * | 2011-06-28 | 2014-05-07 | Vivozon, Inc. | Combination of effective substances causing synergistic effects of multiple targeting and use thereof |
| CN102813660A (en) * | 2012-09-13 | 2012-12-12 | 前郭尔罗斯蒙古族自治县医院 | External medicine for treating skin eczema |
| CN104474008A (en) * | 2014-12-10 | 2015-04-01 | 遵义医学院 | Honeysuckle prickly heat water and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 李璞: "《原来育儿可以这样高效（0-3岁）》", 31 January 2013, 中国妇女出版社 * |
| 王绪前等: "《中药材彩色图谱》", 31 May 2015, 中国医药科技出版社 * |
| 胡本祥等: "《生药学》", 30 September 2013, 西安交通大学出版社 * |
| 郑惠文: "《美容养颜该怎么吃》", 30 June 2014, 中国纺织出版社 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107536756A (en) * | 2017-09-07 | 2018-01-05 | 平舆玛雅生物工程有限公司 | One kind is dispelled miliaria cold cream and preparation method thereof |
| CN107536756B (en) * | 2017-09-07 | 2020-06-12 | 平舆玛雅生物工程有限公司 | Prickly heat removing skin care cream and preparation method thereof |
| CN109363969A (en) * | 2018-10-08 | 2019-02-22 | 广东暨晴生物医药科技有限公司 | A kind of method of comprehensive utilization of wormwood effective component |
| CN109363969B (en) * | 2018-10-08 | 2021-09-17 | 广东暨晴生物医药科技有限公司 | Comprehensive utilization method of effective components of wormwood |
| CN111956558A (en) * | 2020-09-17 | 2020-11-20 | 两面针（江苏）实业有限公司 | Artemisia argyi extract and preparation method and application thereof |
| CN111956558B (en) * | 2020-09-17 | 2022-09-09 | 两面针（江苏）实业有限公司 | Artemisia argyi extract and preparation method and application thereof |
| CN115944662A (en) * | 2022-12-23 | 2023-04-11 | 中南民族大学 | Application of folium artemisiae argyi volatile oil serving as TRPA1 pathway inhibitor in anti-inflammatory and antipruritic medicines |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102949647B (en) | Medical composition for killing bacteria and relieving itching and preparation method of same | |
| CN105395593A (en) | Itching relieving ointment containing folium artemisiae argyi extract and preparation method thereof | |
| CN103182045A (en) | Medicine for treating tinea of feet and hands and preparation method thereof | |
| CN105168385B (en) | A kind of pharmaceutical composition of anti-bacterial, anti-itching and preparation method thereof | |
| CN104922248A (en) | Antibacterial and anti-inflammatory Chinese herbal compound gel and preparation method thereof | |
| CN102283899B (en) | Traditional Chinese medicine external preparation and application thereof | |
| CN106491415A (en) | A kind of pox-eliminating whitening concealer and preparation method thereof | |
| CN115518127B (en) | Antibacterial, anti-inflammatory, detumescence and antipruritic cream and preparation method thereof | |
| CN104666594B (en) | It is a kind of to treat tinea pedis moxibustion item and preparation method thereof | |
| CN102988844A (en) | Gynaecological gel and extraction method thereof | |
| KR20030055154A (en) | A composition for treating a dermatitis and a method of preparing the same | |
| CN114306526B (en) | Compound broadleaf holly leaf traditional Chinese medicine extract and application thereof in preparing shower gel or hand sanitizer | |
| CN108785363A (en) | Application of the yellow wingceltis platymiscium perfume (or spice) material in the drug for preparing treatment microorganism infection | |
| CN108403570A (en) | There are the body condensation and preparation method of improvement to pruitus caused by eczema | |
| CN113797250B (en) | External traditional Chinese medicine composition and preparation method and application thereof | |
| CN101332286B (en) | Traditional Chinese medicine composition for treating skin disease and preparation method thereof | |
| CN107412455A (en) | A kind of natural externally applied pharmaceutical composition for treating fash and preparation method thereof | |
| CN102145072A (en) | Traditional Chinese medicine composition for treating infantile eczema and dermatitis skin diseases | |
| CN106728166A (en) | Treat the Chinese medicine composition of eczema | |
| CN106728069B (en) | Slough-removing and tissue regeneration-promoting paste for treating scalds and burns | |
| CN1970020B (en) | A pharmaceutical composition, preparation process and application thereof | |
| CN101371889A (en) | A set of medicament prescription for treating skin tinea and preparation method thereof | |
| CN100387254C (en) | Suppository of Chinese traditional medicine and preparing method | |
| CN102764330B (en) | Medicine composition for treating cutaneous pruritus and preparation method thereof | |
| CN102552472B (en) | Chinese medicinal preparation for treating vaginitis and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160316 |