CN112889756B - Method for extracting live earthworm mucus - Google Patents
Method for extracting live earthworm mucus Download PDFInfo
- Publication number
- CN112889756B CN112889756B CN201911225008.7A CN201911225008A CN112889756B CN 112889756 B CN112889756 B CN 112889756B CN 201911225008 A CN201911225008 A CN 201911225008A CN 112889756 B CN112889756 B CN 112889756B
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- stainless steel
- steel container
- earthworms
- solution
- salt
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- 210000003097 mucus Anatomy 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 31
- 241000361919 Metaphire sieboldi Species 0.000 title abstract description 37
- 241001233061 earthworms Species 0.000 claims abstract description 58
- 239000012266 salt solution Substances 0.000 claims abstract description 57
- 238000000926 separation method Methods 0.000 claims abstract description 28
- 150000003839 salts Chemical class 0.000 claims abstract description 25
- 239000000243 solution Substances 0.000 claims abstract description 24
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 20
- 238000002156 mixing Methods 0.000 claims abstract description 15
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims abstract description 12
- 239000011780 sodium chloride Substances 0.000 claims abstract description 7
- 239000007864 aqueous solution Substances 0.000 claims abstract description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 92
- 239000010935 stainless steel Substances 0.000 claims description 92
- 230000028327 secretion Effects 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 6
- 239000008399 tap water Substances 0.000 claims description 6
- 235000020679 tap water Nutrition 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 3
- 238000003672 processing method Methods 0.000 claims description 2
- 230000000638 stimulation Effects 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 2
- 239000012535 impurity Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 5
- 230000000857 drug effect Effects 0.000 description 3
- 230000003749 cleanliness Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000700108 Ctenophora <comb jellyfish phylum> Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0332—Earthworms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of earthworm mucus extraction, in particular to a method for extracting living earthworm mucus. The technical scheme adopted by the invention is as follows: the method comprises the following steps: the method comprises the processing steps of preparing three salt solutions with different concentrations, uniformly mixing sodium chloride and calcium sulfate, uniformly mixing the sodium chloride and the calcium sulfate with an aqueous solution to obtain a salt solution, mixing the three different salt solutions according to different proportions of salt content, preparing a solution with the salt content of 3% -4% into a first salt solution, preparing a solution with the salt content of 9% -12% into a second salt solution, preparing a solution with the salt content of 15% -18% into a third salt solution, and respectively carrying out matching stimulation on earthworms through the three different salt solutions. The invention has the advantages that: by adopting three salt solutions with different concentrations to stimulate the earthworms, the mucus separation efficiency in the whole process is higher, and the mucus separation of the earthworms is more thorough, so that the earthworm mucus is more conveniently utilized in the later period.
Description
Technical Field
The invention relates to the technical field of earthworm mucus extraction, in particular to a method for extracting living earthworm mucus.
Background
The earthworm mucus is a bioactive substance actively secreted by earthworms under the stimulation of external environment, contains various medicinal components such as earthworm extract, various amino acids, vitamins and the like, and has great utilization value. Earthworms are coelenterates, which contain large amounts of food and digestive debris. The earthworms can actively spit out substances in the body cavity after being stimulated by the outside, and the substances can pollute the local environment and influence the early quality of mucus.
Disclosure of Invention
The invention aims to provide a method for extracting living earthworm mucus, which adopts a brand new separation method, can completely separate impurities in earthworms by processing the collected earthworms step by step, then carries out secondary separation treatment on the earthworms after the impurities are separated, and finally realizes high-purity separation of the living earthworm mucus in a saline solution stimulation mode, so that not only can pure earthworm mucus be collected, but also the overall cleanliness of the earthworms can be higher, the whole process adopts three saline solutions with different concentrations to stimulate the earthworms, the mucus separation efficiency in the whole process is higher, the mucus separation of the earthworms is more thorough, the later-stage utilization of the earthworm mucus is more convenient, and the drug effect of the mucus in the later stage is more reliable. The technical scheme of the invention is as follows:
a method for extracting mucus of living earthworms is characterized by comprising the following steps: 1) The processing step comprises the steps of uniformly mixing sodium chloride and calcium sulfate, then uniformly mixing the sodium chloride and the calcium sulfate with an aqueous solution to obtain a salt solution, mixing the three different salt solutions according to different salt content proportions, preparing the solution with the salt content of 3% -4% into a first salt solution, preparing the solution with the salt content of 9% -12% into a second salt solution, and preparing the solution with the salt content of 15% -18% into a third salt solution; 2) Firstly, mixing the earthworms in the first stainless steel container with the first salt solution, standing for 15 minutes, then putting the whole first stainless steel container into a second stainless steel container, and adding tap water into the second stainless steel container; adding a third salt solution into the first stainless steel container, stirring in the adding process, controlling the adding amount of the third salt solution until the solution surface is flush with the upper end surface of the first stainless steel container, waiting for 3-5 minutes, and allowing the earthworms to completely climb out of the first stainless steel container to the second stainless steel container to finish the first separation operation; 4) Firstly, fishing out the earthworms obtained in the previous step through a screen, putting the fished earthworms into a third stainless steel container, wherein the distance from the upper end position of the earthworms in the third stainless steel container to the upper end opening of the third stainless steel container is 18-22cm, and then adding a second saline solution into the third stainless steel container to enable the saline solution to completely cover the earthworms in the third stainless steel container; 5) A step of performing secondary separation on earthworms, which is to firstly stand the third stainless steel container for 10 minutes, then put the third stainless steel container into a fourth stainless steel container, add tap water into the fourth stainless steel container, then add a third salt solution into the third stainless steel container, perform stirring operation in the adding process, control the adding amount of the third salt solution until the solution surface is flush with the upper end surface of the third stainless steel container, wait for 3-5 minutes, allow the earthworms to completely climb out of the third stainless steel container into the fourth stainless steel container, and complete secondary separation operation, 6), and collect the secretion solution in the third stainless steel container; 7) And filtering and collecting the earthworms in the fourth stainless steel container.
Further, the salt content of the first salt solution is 3%, 3.2%, 3.5%, 3.6%, 3.8%, 4%.
Further, the salt content of the second salt solution is 9.5%, 10%, 10.3%, 11.5%, 12%.
Further, the salt content of the third salt solution is 15%, 15.3%, 15.9%, 16.5%, 17.2%, 18%.
Further, the second stainless steel container and the fourth stainless steel container are both circular stainless steel basins.
Further, the second stainless steel container and the fourth stainless steel container are different from the first stainless steel container and the third stainless steel container.
The invention has the beneficial effects that:
according to the invention, a brand new separation method is adopted, the collected earthworms are subjected to step-by-step treatment, impurities in the earthworms can be completely separated, then the earthworms after the impurities are separated are subjected to secondary separation treatment, and finally the living earthworm mucus is separated in a high-purity mode through salt solution stimulation, so that not only can the pure earthworm mucus be collected, but also the whole cleanness of the earthworms can be higher, the earthworms are stimulated through three salt solutions with different concentrations in the whole process, the mucus separation efficiency in the whole process is higher, the earthworm mucus separation is more thorough, the earthworm mucus is more conveniently utilized in the later stage, and the drug effect of the mucus is more reliable when the earthworm is used in the later stage.
Detailed Description
The utility model provides a method for draw live body earthworm mucus, it adopts brand-new separation method, through carrying out substep processing with the earthworm of collecting, can carry out complete separation with the internal impurity of earthworm, then carry out secondary separation processing through the earthworm after will separating impurity, finally realize the high purity separation to live body earthworm mucus through the amazing mode of salt solution, not only can collect pure earthworm mucus, and can also make earthworm's whole cleanliness higher, whole process is amazing the earthworm through adopting three kinds of salt solution of different concentration, whole process's mucus separation efficiency is higher, the mucus separation of earthworm is more thorough, thereby make later stage to earthworm mucus utilization more convenient, it is more reliable to ensure the mucus drug effect when later stage uses. The invention comprises the following steps: 1) The processing method comprises the steps of preparing three salt solutions with different concentrations, uniformly mixing sodium chloride and calcium sulfate, then uniformly mixing the three salt solutions with an aqueous solution to obtain a salt solution, mixing the three different salt solutions according to different salt content proportions, preparing the solution with the salt content of 3% -4% into a first salt solution, preparing the solution with the salt content of 9% -12% into a second salt solution, and preparing the solution with the salt content of 15% -18% into a third salt solution; the three salt solutions are added through different steps when in use, so that the earthworms can better secrete different substances in the body, the integral purity during secretion can be better guaranteed, and the secretion efficiency can be integrally improved. 2) The earthworms are placed in a first stainless steel container in the step, and the distance between the upper end position of the earthworms in the first stainless steel container and the upper end opening of the first stainless steel container is 18-22cm, so that the earthworms can have a larger shunting space in the container, the earthworms can be thoroughly secreted, and the overall efficiency of secretion is guaranteed. Adding the first salt solution into the first stainless steel container, and 3) carrying out primary separation treatment on earthworms, firstly, mixing the earthworms in the first stainless steel container with the first salt solution, standing for 15 minutes, then putting the whole first stainless steel container into a second stainless steel container, and adding tap water into the second stainless steel container; and then adding a third salt solution into the first stainless steel container, stirring in the adding process, controlling the adding amount of the third salt solution until the solution surface is flush with the upper end surface of the first stainless steel container, waiting for 3-5 minutes, and enabling the earthworms to completely climb out of the first stainless steel container into the second stainless steel container to complete the first separation operation, so that the earthworms can completely separate out the impurities in the body, and the secretion of the important secretion substances in the later stage is facilitated. 4) And step (3) earthworm secretion, namely firstly fishing out the earthworm obtained in the previous step through a screen, putting the fished earthworm into a third stainless steel container, wherein the distance from the upper end position of the earthworm in the third stainless steel container to the upper end opening of the third stainless steel container is 18-22cm, and then adding a second saline solution into the third stainless steel container to enable the saline solution to completely cover the earthworm in the third stainless steel container, so that the earthworm can start to secrete internal mucus, and the secreted viscosity is dissolved into the solution. 5) And (3) carrying out secondary separation on the earthworms, namely firstly standing the third stainless steel container for 10 minutes, then putting the third stainless steel container into a fourth stainless steel container integrally, adding tap water into the fourth stainless steel container, then adding a third salt solution into the third stainless steel container, stirring in the adding process, controlling the adding amount of the third salt solution until the solution surface is flush with the upper end surface of the third stainless steel container, waiting for 3-5 minutes, and making the earthworms completely climb out of the third stainless steel container into the fourth stainless steel container, so that secondary separation is finished, and thus the mucus separation of the earthworms is finished. 6) The step of collecting the secretion solution in the third stainless steel container, and the step of 7) filtering and collecting the earthworms in the fourth stainless steel container, so that the secretion solution and the earthworms can be completely separated and can be respectively utilized, and the obtained products can also be respectively sold.
Preferably, the salt content of the first salt solution is 3%, 3.2%, 3.5%, 3.6%, 3.8%, 3.9%, 4%; the salt content of the second salt solution is 9.5%, 10%, 10.3%, 11.5%, 12%; the salt content of the third salt solution is 15%, 15.3%, 15.9%, 16.5%, 17.2% and 18%; after the earthworm secretion feed is proportioned and mixed, the earthworm secretion effect can be better ensured when the earthworm secretion feed is used.
Preferably, the second stainless steel container and the fourth stainless steel container are both round stainless steel basins, and the two containers can have a larger space when being used, so that the earthworms can be taken out more conveniently, and the taking-out efficiency of the earthworms can be guaranteed to be higher.
Preferably, the second stainless steel container and the fourth stainless steel container are different from the first stainless steel container and the third stainless steel container, so that different separated objects can be effectively distinguished in the whole treatment process, and the safety of the whole process is better.
The foregoing is a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications or substitutions can be made without departing from the principle of the present invention, and these modifications or substitutions should also be considered as the protection scope of the present invention.
Claims (6)
1. A method for extracting mucus of living earthworms is characterized by comprising the following steps: 1) The processing method comprises the steps of preparing three salt solutions with different concentrations, uniformly mixing sodium chloride and calcium sulfate, then uniformly mixing the three salt solutions with an aqueous solution to obtain a salt solution, mixing the three different salt solutions according to different salt content proportions, preparing the solution with the salt content of 3% -4% into a first salt solution, preparing the solution with the salt content of 9% -12% into a second salt solution, and preparing the solution with the salt content of 15% -18% into a third salt solution; 2) Firstly, mixing the earthworms in the first stainless steel container with the first salt solution, standing for 15 minutes, then putting the whole first stainless steel container into a second stainless steel container, and adding tap water into the second stainless steel container; adding a third salt solution into the first stainless steel container, stirring in the adding process, controlling the adding amount of the third salt solution until the solution surface is flush with the upper end surface of the first stainless steel container, waiting for 3-5 minutes, and allowing the earthworms to completely climb out of the first stainless steel container to the second stainless steel container to finish the first separation operation; 4) Firstly, fishing out the earthworms obtained in the previous step through a screen, putting the fished earthworms into a third stainless steel container, wherein the distance from the upper end position of the earthworms in the third stainless steel container to the upper end opening of the third stainless steel container is 18-22cm, and then adding a second saline solution into the third stainless steel container to enable the saline solution to completely cover the earthworms in the third stainless steel container; 5) A step of performing secondary separation on earthworms, which is to firstly stand the third stainless steel container for 10 minutes, then put the third stainless steel container into a fourth stainless steel container, add tap water into the fourth stainless steel container, then add a third salt solution into the third stainless steel container, perform stirring operation in the adding process, control the adding amount of the third salt solution until the solution surface is flush with the upper end surface of the third stainless steel container, wait for 3-5 minutes, allow the earthworms to completely climb out of the third stainless steel container into the fourth stainless steel container, and complete secondary separation operation, 6), and collect the secretion solution in the third stainless steel container; 7) And filtering and collecting the earthworms in the fourth stainless steel container.
2. The method for extracting mucus of living earthworms as claimed in claim 1, wherein: the salt content of the first salt solution is 3%, 3.2%, 3.5%, 3.6%, 3.8% and 4%.
3. The method for extracting mucus of living earthworms as claimed in claim 1, wherein the method comprises the steps of: the salt content of the second salt solution is 9.5%, 10%, 10.3%, 11.5%, 12%.
4. The method for extracting mucus of living earthworms as claimed in claim 1, wherein: the salt content of the third salt solution is 15%, 15.3%, 15.9%, 16.5%, 17.2%, 18%.
5. The method for extracting mucus of living earthworms as claimed in claim 1, wherein: the second stainless steel container and the fourth stainless steel container are both round stainless steel basins.
6. The method for extracting mucus of living earthworms as claimed in claim 1, wherein: the second and fourth stainless steel containers are different from the first and third stainless steel containers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911225008.7A CN112889756B (en) | 2019-12-03 | 2019-12-03 | Method for extracting live earthworm mucus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911225008.7A CN112889756B (en) | 2019-12-03 | 2019-12-03 | Method for extracting live earthworm mucus |
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| Publication Number | Publication Date |
|---|---|
| CN112889756A CN112889756A (en) | 2021-06-04 |
| CN112889756B true CN112889756B (en) | 2023-03-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911225008.7A Active CN112889756B (en) | 2019-12-03 | 2019-12-03 | Method for extracting live earthworm mucus |
Country Status (1)
| Country | Link |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100363197B1 (en) * | 2000-07-11 | 2002-12-05 | (주)이오바이오텍 | the extracting method of earthworm's body fluids using water contained activated oxygen |
| WO2009078348A1 (en) * | 2007-12-14 | 2009-06-25 | Mihara L R Institute Co.Ltd | Method of producing liquid earthworm extract and method of producing dry earthworm powder |
| CN106634131A (en) * | 2016-11-10 | 2017-05-10 | 仇颖超 | Preparation method of environment-friendly marine antifouling coating |
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| SU1108102A1 (en) * | 1982-08-27 | 1984-08-15 | Институт Ботаники Ан Казсср | Method of obtaining peroxidase |
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| KR100301306B1 (en) * | 1998-12-22 | 2001-10-29 | 홍정숙 | Method for extracting yellow mucus from earthworm and cosmetic composition using the same |
| CN1414095A (en) * | 2002-08-30 | 2003-04-30 | 宁夏金丰源实业有限责任公司 | Preparation method of high actirity earthworm kinase dry powder |
| CN100352362C (en) * | 2003-12-03 | 2007-12-05 | 孙振钧 | Earthworm active peptide antibacerial feed material and its production method |
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| KR100363197B1 (en) * | 2000-07-11 | 2002-12-05 | (주)이오바이오텍 | the extracting method of earthworm's body fluids using water contained activated oxygen |
| WO2009078348A1 (en) * | 2007-12-14 | 2009-06-25 | Mihara L R Institute Co.Ltd | Method of producing liquid earthworm extract and method of producing dry earthworm powder |
| CN106634131A (en) * | 2016-11-10 | 2017-05-10 | 仇颖超 | Preparation method of environment-friendly marine antifouling coating |
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