KR100301306B1 - Method for extracting yellow mucus from earthworm and cosmetic composition using the same - Google Patents

Method for extracting yellow mucus from earthworm and cosmetic composition using the same Download PDF

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KR100301306B1
KR100301306B1 KR1019980057208A KR19980057208A KR100301306B1 KR 100301306 B1 KR100301306 B1 KR 100301306B1 KR 1019980057208 A KR1019980057208 A KR 1019980057208A KR 19980057208 A KR19980057208 A KR 19980057208A KR 100301306 B1 KR100301306 B1 KR 100301306B1
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earthworm
weight
powder
yellow mucus
cosmetic composition
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KR19990024216A (en
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홍정숙
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/62Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/02Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0212Face masks
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Zoology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Cosmetics (AREA)

Abstract

PURPOSE: Provided are a method for extracting yellow mucus from earthworm, and a cosmetic composition using the same, thereby curing pimple. CONSTITUTION: A method for extracting yellow mucus from interior earthworm comprises the steps of: (a) washing earthworm; (b) immersing the washed earthworm into alcoholic aqueous solution of 15-20 wt.% then separating crude extract solution from them; (c) filtering the crude extract; (d) adding sliced onion of 3 wt.% and licorice of 1.5 wt.% thereinto and then heating them at 80-90 deg.C for 2-3 hours; (e) putting the resultant into siphon bottle and freezing them, to eliminate remainings therefrom.

Description

생지렁이 체내로부터 황색점액물질 추출방법 및 이를 이용한 화장료 조성물Extraction method of yellow mucus material from body of earthworm and cosmetic composition using same

본 발명은 생지렁이 체내에 존재하는 황색점액물질의 추출방법에 관한 것이다. 더욱 상세히 설명하면, 본 발명은 "토룡"이라고도 하는 지렁이의 체내에 항시적으로 존재하는 황색점액물질을 역겨운 냄새 없이 효과적으로 추출하는 신규하고 진보성이 구유된 방법에 관한 것이다.The present invention relates to a method for extracting yellow mucus present in the body of a live earthworm. In more detail, the present invention relates to a novel and progressive trough method for effectively extracting yellow mucus, which is always present in the body of an earthworm, also called "tortoise," without disgusting smell.

지렁이는 옛부터 동양 여러나라에서 약제로 사용되어 왔다. 천연약품사전, 오꾸다다꾸오편, 소화 61. 4. 15. 히로가와 서점발행, P215에는 지렁이 추출물이 해열제, 진통제, 이뇨제, 해독제의 용도로 보고된 바 있으며, 국내특허 공고번호 93-2010호에는 당뇨병 치료제로 공개되고 있다.Earthworms have been used as medicine in many Asian countries since ancient times. Natural Drug Dictionary, Oda Koda, Digestion 61. 4. 15. Published in Hirogawa Bookstore, P215 has been reported that earthworm extracts are used for antipyretics, analgesics, diuretics, antidote, and Korean Patent Publication No. 93-2010 It is disclosed as a diabetes treatment.

또, 일본 게이메이(주) 대표이사 이시이요이찌의 지렁이의 단백질 및 지질을 주성분으로하는 건강식품 및 그 제조법에 관한 일본특허 공개공보 소 59-216572 호(84. 12. 6)에 의하면 지렁이 건조분말의 건강상 효과가 입증된 바 있다.In addition, according to Japanese Patent Publication No. 59-216572 (84. 12. 6) concerning health food mainly containing proteins and lipids of earthworms of Keimei, Japan's representative director, Ishiyoiichi, and its manufacturing method The health effects of the powder have been demonstrated.

이밖에도 일본 바이오케미파(주)의 연구팀은 지렁이를 열탕처리한 엑기스는 사람이 마시거나 피부에 바르면 건성피부를 윤활하게 하고 피부의 수축작용을 도와 탄력성이 소성되며 그 결과 피부를 부드럽게 하고 보습작용이 있음을 입증하였다.In addition, the research team of Nippon Biochemopa Co., Ltd. extracts the earthworm boiled lubricated dry skin when people drink or apply it on the skin, help the skin contraction, plasticity elasticity, resulting in softening and moisturizing the skin. Proved.

이와 같은 약효성분이 있는 물질은 지렁이 전체를 건조하여 사용하거나 또는 지렁이 체내의 분토를 제거한 다음 나머지 유기체만을 건조하여 사용하거나 지렁이 전체를 수성용매 등의 추출제에 의하여 추출처리하여 사용하는 방법이 보고되어 왔다. 그러나, 상기와 같이 제조된 지렁이 건조분말 또는 지렁이 추출물은 역겨운 냄새로 인하여 그 식용 또는 타용도로의 사용이 지극히 제한되어 왔다.For substances with such active ingredients, it is reported that the whole earthworm is used by drying the earthworm or removing the soil in the earthworm body, and then only the remaining organisms are dried or the whole earthworm is extracted and treated with an extractant such as an aqueous solvent. come. However, the earthworm dry powder or earthworm extract prepared as described above has been extremely limited in its use as an edible or other purpose due to the disgusting smell.

따라서, 본 발명의 목적은 상기와 같은 종래의 단점을 개선하고자 안출한 것으로 생지렁이 체내에 존재하는 황색점액물질을 효과적으로 추출하고 그 냄새를 제거함에 있다. 본 발명의 다른 목적은 냄새가 제거된 지렁이로부터 유래하는 황색점액물질을 유효성분으로 하는 여드름 치료용 화장료 조성물을 제공함에 있다.Therefore, an object of the present invention is to devise to improve the above disadvantages, and to effectively extract the yellow mucus material present in the body of the earthworm and remove its smell. Another object of the present invention to provide a cosmetic composition for treating acne as a yellow mucus material derived from the earthworm odor is removed as an active ingredient.

본 발명의 또 다른 목적은 1차 여과 후 잔류한 지렁이에 규석분말을 살포 흡착시킨 후 원심분리하여 무색점액물질을 얻은 다음 이에 황토분말 또는 오이분말과 혼합한 화장료 팩을 제공함에 있다.Still another object of the present invention is to provide a cosmetic pack mixed with ocher powder or cucumber powder, after which the silica powder is sprayed and adsorbed on the earthworm remaining after the first filtration to obtain a colorless slime material.

본 발명의 상기 목적은 채취된 지렁이를 그물망 위에 3∼4일간 방치한 후, 분토를 제거하고 세정수로 세척한 다음 15∼20%의 알콜수용액에 침지하여 지렁이 생체와 점액물질을 분리한 다음 이 추출액을 여과하여 조추출액을 얻고, 이 조추출액에 양파와 감초를 첨가한 후 80∼90℃에서 2∼3시간 열탕처리한 다음 재차 여과하여 황색점액물질을 얻고 이를 유효성분으로 하는 조성물을 여드름 치료제로 사용하여 이를 평가하고, 아울러 1차 여과 잔류물에 규석분말을 살포 흡착한 후 원심분리하여 무색점액물질을 얻고 이에 황토분말 또는 오이분말과 혼합하여 화장료 팩을 제조한 다음 사용평가함으로써 달성하였다.The object of the present invention is to leave the collected earthworms on the net for 3 to 4 days, remove the soil and wash with washing water and then immersed in 15-20% alcohol aqueous solution to separate the earthworm body and mucus material The crude extract was filtered to obtain crude extract. Onion and licorice were added to the crude extract, and then heated at 80 to 90 ° C. for 2 to 3 hours, followed by filtration to obtain a yellow slime material. Evaluate this by using, as well as by spraying and adsorbing the silica powder to the primary filtration residue and centrifugation to obtain a colorless mucus material was obtained by mixing with ocher powder or cucumber powder to prepare a cosmetic pack and then evaluating use.

이하, 본 발명의 구체적인 구성 및 작용을 설명한다.Hereinafter, the specific configuration and operation of the present invention.

도 1은 본 발명의 바람직한 실시예를 보인 생지렁이 체내로부터 황색점액물질의 추출공정을 나타낸다.Figure 1 shows the extraction process of the yellow mucus material from the living earthworm body showing a preferred embodiment of the present invention.

본 발명은 도 1의 단계별 설명에서 보는 바와 같이 채취하여 수집된 생지렁이를 3∼4일정도 1∼2㎜ 망체위에 방치하여 분토(糞土)를 제거하는 단계와; 분토가 제거된 생지렁이를 물로 2∼3회 가볍게 세정하는 단계와; 세정한 생지렁이를 15∼20%(중량) 알콜수용액에 침지하여 지렁이 몸체와 조추출액을 분리하는 단계와; 추출된 엑기스를 여과하는 단계와; 여과한 추출물에 세절한 양파와 감초를 첨가한 후 80∼90℃에서 2∼3시간 열탕처리하는 단계와; 열탕처리한 추출물을 사이폰(siphon)병에 넣어 -4℃에서 냉동시켜 잔류물을 하부에서 제거하는 재여과 단계와 이와 별도로 1차 여과 잔류물에 규석분말을 첨가하는 단계와; 이를 원심분리하는 단계와; 상층액에 황토 또는 오이분말을 첨가하는 단계로 구성됨을 특징으로 한다.The present invention comprises the steps of removing the soil by leaving the collected live earthworms on a 1-2 mm network about 3 to 4 days as shown in the step-by-step description of Figure 1; Gently washing the earthworms from which the soil has been removed with water two to three times; Dipping the cleaned earthworm in 15-20% (weight) alcohol aqueous solution to separate the earthworm body and the crude extract; Filtering the extracted extract; Adding a fine onion and licorice to the filtered extract, followed by boiling water treatment at 80 to 90 ° C. for 2 to 3 hours; Adding a silica powder to the primary filtration residue separately from the re-filtration step of putting the heated extract in a siphon bottle and freezing at −4 ° C. to remove the residue from the bottom; Centrifuging it; It is characterized by consisting of the step of adding ocher or cucumber powder to the supernatant.

이하, 본 발명의 구체적인 구성과 작용을 실시예를 들어 설명하지만 본 발명의 권리범위가 이들 실시예에만 제한되지 않는다.Hereinafter, the specific configuration and operation of the present invention will be described with reference to Examples, but the scope of the present invention is not limited only to these Examples.

실시예 1Example 1

채취된 생지렁이 1㎏을 1㎜ 망체위에 3일간 방치하여 분토를 제거하였다.1 kg of the collected earthworms was left on a 1 mm mesh for 3 days to remove the soil.

분토가 제거된 생지렁이를 깨끗한 물로 2∼3회 가볍게 세정하고 세정한 생지렁이를 15% 알콜수용액에 침지하여 지렁이 몸체와 조추출액을 분리하여 조추출액 2,750g을 얻었다. 조추출액 100부(중량)에 대하여 세정한 양파 3부(중량)와 감초 1.5부(중량)를 첨가한 후 가열솥에서 80℃에서 3시간 열탕처리하였다. 열탕처리한 조추출물(Crude extract)을 사이폰병(siphon)에 투입하여 -4℃로 냉동하였다. 냉동 후 사이폰의 하부 부분만을 가열시켜 잔류물을 제거하여 물리적으로 재여과한 황색의 지렁이 추출물을 얻었다.The earthworms from which the soil was removed were lightly washed 2-3 times with clean water, and the washed earthworms were immersed in 15% alcohol solution to separate the earthworm body and the crude extract to obtain crude extract 2,750g. To 100 parts (weight) of crude extract, 3 parts (weight) of washed onion and 1.5 parts (weight) of licorice were added, followed by boiling water treatment at 80 ° C. for 3 hours. Crude extract treated with boiling water was added to a siphon bottle and frozen at -4 ° C. After freezing, only the lower part of the siphon was heated to remove the residue, thereby obtaining a yellow earthworm extract that was physically refiltered.

실시예 2Example 2

지렁이 조추출액을 얻기 위하여 알콜 20% 수용액을 사용하고 열탕처리온도를 90℃에서 2시간 수행한 것 외에는 상기 실시예 1과 동일하게 하여 황색의 지렁이 추출물을 얻었다.A yellow earthworm extract was obtained in the same manner as in Example 1 except that 20% aqueous alcohol was used and a boiling water treatment temperature was performed at 90 ° C. for 2 hours to obtain an earthworm crude extract.

본 발명의 실시예에서 사용한 역겨운 냄새 제거를 위한 원료물질은 양파가 사용되지만 양파외에도 S-S 결합 단백질이 함유된 어떤 원료라도 좋다. 예컨대 계피, 생강, 마늘이 사용될 수 있다.As a raw material for removing the disgusting smell used in the embodiment of the present invention, onion is used, but any raw material containing S-S binding protein besides onion may be used. For example cinnamon, ginger and garlic can be used.

상기 실시예에서 알콜의 농도를 5∼10% 수용액으로 처리하면 지렁이가 90% 생존하고 15∼20% 수용액으로 처리하면 80% 생존한다.In this embodiment, the earthworm survives 90% when treated with a 5-10% aqueous solution and 80% when treated with a 15-20% aqueous solution.

실시예 3Example 3

상기 실시예 1에서 1차 여과 후 잔류된 지렁이 표피에 무색점액물질을 얻기 위하여 규석분말을 잔류물량의 200부(중량) 첨가한 후 1,000×g의 중력으로 원심분리한 다음 얻은 상청액 875g을 얻었다. 상청액 400g에 황토분말 100부(중량)를 첨가하여 화장료 팩을 제조하였다.In order to obtain a colorless viscous substance on the earthworm epidermis remaining after the first filtration in Example 1, the silica powder was added 200 parts (weight) of the residual amount and centrifuged at a gravity of 1,000 × g to obtain 875 g of the supernatant. 100 parts (weight) of ocher powder was added to 400 g of the supernatant to prepare a cosmetic pack.

실시예 4Example 4

상기 실시예 3과 동일하게 하되 상청액에 400g에 오이분말 100부(중량)를 첨가하여 화장료 팩을 제조하였다.As in Example 3, 100 parts (weight) of cucumber powder was added to 400 g of the supernatant to prepare a cosmetic pack.

본 발명 추출방법에 의해 생산된 생지렁이 추출물을 여드름 치료제로 사용한 결과 100% 치료효과를 얻었다. 이하에서는 본 발명 생지렁이 유래의 황색 및 무색물질의 사용예를 보인 것이다.As a result of using the earthworm extract produced by the extraction method of the present invention as an acne treatment, a 100% therapeutic effect was obtained. Hereinafter will be shown an example of the use of yellow and colorless material derived from the earthworm earthworm of the present invention.

사용실시예 1Example 1

여드름으로 고민하고 있는 남녀 고등학생 각 5명씩에 대하여 물비누 100부(중량)에 대하여 상기 실시예 1 또는 실시예 2에서 조제한 지렁이 추출물 100부(중량)를 혼합하여 1일 2회 염증이 생성된 부분만 3일간 도포한 결과 남자 4명, 여자 5명이 완치되었고, 남자 1명은 부분치료되었다.Only 100 parts (weight) of the earthworm extract prepared in Example 1 or Example 2 were mixed with 100 parts (weight) of water soap for each of 5 male and female high school students who suffered from acne. Three days of application resulted in four men and five women cured and one man partially treated.

사용실시예 2Example 2

상기 사용실시예 1의 실험에 응답한 학생들에게 실시예 1 또는 실시예 2의 지렁이 추출물에 대하여 역겨운 냄새 유무와 본 발명품의 사용중 발진현상 유무에 대하여 질문한 결과 단 한 학생도 역겨운 냄새나 발진현상을 토로하지 않았다.As a result of asking the students responding to the experiment of Example 1 about the presence of disgusting odor and the rash phenomenon during use of the present invention with respect to the earthworm extract of Example 1 or Example 2, even one student may experience disgusting odor or rash phenomenon. Did not toro

사용실시예 3Example 3

상기 사용실시예 3과 실시예 4에서 제조된 황토분말이 함유된 화장료 팩과 오이분말이 함유된 화장료 팩을 30대 여성 각 5명과 40대 여성 각 5명을 무작위 추출하여 매일 저녁 1회 7일간 사용 후 기존제품과 비교한 실험결과는 다음 표 1과 같다.The cosmetic packs containing ocher powder and cucumber powder prepared in Examples 3 and 4 were used to randomly extract 5 women in their 30s and 5 women in their 40s, once every evening for 7 days. The experimental results compared with the existing products after use are shown in Table 1 below.

비교실험결과 (단위 : 점수)Comparative Experiment Result (Unit: Score) 구 분division 보습성Moisturizing 피부탄력성Skin elasticity 잔주름 제거효과Fine wrinkle removal effect 실시예 3 제품Example 3 Products 5050 5050 5050 실시예 4 제품Example 4 Products 5050 5050 5050 주 : 기존제품보다 극히우수(5), 우수(3), 비슷(2.5), 못하다(1)으로 평가함Note: It is rated as extremely excellent (5), excellent (3), similar (2.5), and poor (1) than existing products.

실험결과 본 발명 제품은 기존 화장료 제품에 비하여 보습성, 피부탄력성 및 잔주름 제거효과에 있어서 극히 우수한 것을 알 수 있다.As a result of the experiment it can be seen that the product of the present invention is extremely excellent in moisturizing, skin elasticity and fine wrinkles removal effect compared to the existing cosmetic products.

이상의 실시예와 사용실시예를 통하여 설명한 바와 같이 본 발명 지렁이 추출물은 알콜과 앙파와 감초를 사용하여 생지렁이로부터 역겨운 냄새가 없는 양질의 황색점액물질을 추출할 수 있는 효과와 신규한 여드름 치료제를 제공하는 효과가 있으며 이와 별도로 황토분말 또는 오이분말이 첨가된 신규한 형태의 화장료 팩을 제공하는 효과가 있으므로 생물의약 및 화장품 산업상 매우 유용한 발명인 것이다.As described through the above examples and use examples, the earthworm extract of the present invention uses alcohol, enamel and licorice to provide a novel acne treatment and an effect of extracting a good yellow mucus material without a disgusting smell from raw earthworms. In addition, the ocher powder or cucumber powder is added to the novel form of the cosmetic pack is added to the effect of providing a very useful invention in the biopharmaceutical and cosmetics industry.

Claims (3)

생지렁이로부터 분토(糞土)를 제거하고 세정한 다음 황색점액물질을 추출하는 방법에 있어서, 상기 세정한 생지렁이를 15∼20%(중량) 알콜수용액에 침지하여 조추출액을 분리하는 단계와; 상기 조추출물을 여과하는 단계와; 상기 여액에 세절한 양파와 감초를 각각 3부(중량)와 1.5부(중량) 첨가한 다음 80∼90℃에서 2∼3시간 열탕처리하는 단계와; 상기 열탕처리한 여액을 사이폰(siphon)병에 넣어 -4℃에서 냉동시켜 잔류물을 하부에서 제거하는 재여과 단계로 구성됨을 특징으로 하는 생지렁이 체내로부터 횡색점액물질 추출방법.What is claimed is: 1. A method of removing clay soil from raw earthworms, washing and extracting yellow mucus material, the method comprising: separating crude extracts by dipping the washed earthworms in an aqueous 15-20% (weight) alcohol aqueous solution; Filtering the crude extract; Adding 3 parts (weight) and 1.5 parts (weight) of finely cut onions and licorice to the filtrate, and then hot water treatment at 80 to 90 ° C. for 2 to 3 hours; Putting the heated filtrate in a siphon bottle (siphon) frozen at -4 ℃ to remove the residue from the bottom, characterized in that it comprises a re-filtration step of extracting transverse mucus material from the body of the earthworm body. 제 1 항 기재의 황색점액물질을 유효성분으로 하는 화장료 조성물.A cosmetic composition comprising the yellow mucus substance according to claim 1 as an active ingredient. 제 1 항 기재의 1차 여과 잔류물에 규석분말을 첨가하고 이를 원심분리한 후 상층액에 황토분말 또는 오이분말중 어느 하나를 선택하여 첨가함을 특징으로 하는 화장료 팩.A cosmetic pack, characterized in that the silica powder is added to the primary filtration residue according to claim 1, and then centrifuged to add either ocher powder or cucumber powder to the supernatant.
KR1019980057208A 1998-12-22 1998-12-22 Method for extracting yellow mucus from earthworm and cosmetic composition using the same KR100301306B1 (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006136540A3 (en) * 2005-06-20 2007-04-26 Incubadora De Negocios Gesta M Human skin regenerating ointment comprising annelid extracts
CN112889756A (en) * 2019-12-03 2021-06-04 泰州市春光生态农业发展有限公司 Novel high-efficiency method for extracting living earthworm mucus

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100367838B1 (en) * 2000-07-12 2003-01-10 (주)이오바이오텍 the cosmetics using extracted material in earthworm
KR100557892B1 (en) * 2002-03-29 2006-03-10 보은군 loess include earthworm powder and a method of processing thereof
EP3603653B1 (en) * 2017-03-21 2023-08-23 Well Stone Co. Production method of therapeutic agent for skin lesions, and its use

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006136540A3 (en) * 2005-06-20 2007-04-26 Incubadora De Negocios Gesta M Human skin regenerating ointment comprising annelid extracts
CN112889756A (en) * 2019-12-03 2021-06-04 泰州市春光生态农业发展有限公司 Novel high-efficiency method for extracting living earthworm mucus

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