KR102539176B1 - Manufacturing method of horse oil flavonoid complex extract - Google Patents

Abstract

The present invention relates to a method for producing a horse oil flavonoid complex extract, which includes the steps of treating horse fat dough with purified water at a certain temperature to obtain a horse oil extract in which the water layer and foreign matter are purified (S10), bee pollen extract 10g, gardenia, Mixing 10 g of herbal extracts made of pomegranate and cloves to obtain a powdery flavonoid extract (S20, S30), mixing 1000 g of horse oil extract and 20 g of the flavonoid extract and putting it into a wet nano grinder at 25 ° C. for 2 hours to nano It is characterized by preparing a complex extract having excellent efficacy in skin health and beauty by containing a large amount of active ingredients of horse oil and flavonoids as it includes a step (S40) of obtaining a complex extract by grinding into particles.

Description

Manufacturing method of horse oil flavonoid complex extract {MANUFACTURING METHOD OF HORSE OIL FLAVONOID COMPLEX EXTRACT}

The present invention relates to a method for producing a horse oil flavonoid composite extract, and more particularly, to effectively extract and compose horse oil and flavonoids containing a large amount of various ingredients beneficial to the skin to prepare a composite extract that derives skin health and beauty effects It relates to a technique for using it as a raw material for a natural cosmetic composition.

Recently, as the frequency of exposure to various chemicals as well as the deterioration of air and water quality increases, the number of patients complaining of various skin diseases such as dermatitis or allergies is increasing day by day.

In particular, people with sensitive skin often show hypersensitivity reactions to cosmetic compositions or treatments made of chemical compositions or worsen symptoms, so cosmetic compositions made of natural ingredients that are relatively safe to the human body are being provided.

For example, Korean Patent No. 10 - 0377262 discloses a mixed solvent in which Injin mugwort is mixed with ethyl alcohol: 1,3-butylene glycol: water in a weight ratio of 1: 1: 1 to 1: 5: 8 based on the total weight of the cosmetic composition. It constitutes a cosmetic composition for atopic skin containing 0.1 to 5% by weight of mugwort extract extracted using, and Korean Patent Registration No. 10 - 0517465 discloses that in a conventional cosmetic composition, Ganoderma lucidum, Radix root bark, licorice, Baekbongryeong, Baekjima and Baeknyeoncho It constitutes an herbal cosmetic composition for atopic skin containing 0.5 to 5.0% by weight of the extracted herbal plant extract.

On the other hand, horse oil is a natural oil extracted from horse fat through a refining and aging process. It contains the most unsaturated fatty acids among animal and vegetable oils, is rich in palitoleic acid ceramide and linoleic acid, and has high molecular activity, so it has high penetration into the skin. It is a moisturizing oil.

As an example of a cosmetic composition containing a horse oil component, Korean Patent Registration No. 10 - 1614500 includes purified horse oil containing horse oil extract and citrus peel extract as active ingredients, and purified horse oil is formed by mixing horse oil extract with citrus peel extract. A cosmetic composition produced by distilling the mixture under reduced pressure at 40 to 50 ° C is constituted.

As described above, in order to use horse oil as a cosmetic, a predetermined purification process must be performed. For example, in Korean Patent Registration No. 10 - 0530215, slaughtered horse meat is heated with water at a temperature of 80 to 120 ° C to remove fat from the fat. A raw horse oil extraction step of extracting liquefied horse oil, a degumming step of treating raw horse oil with phosphoric acid at a concentration of 0.1% to remove viscous substances contained in raw horse oil, and then washing with water at 60 to 70 ° C, and phosphoric acid treatment A deoxidation step in which caustic soda diluted to a concentration of 2.5% is added to the raw material horse oil and washed with water at 60 to 70 ° C, and acid clay of 5% by weight is added to remove moisture present in the raw horse oil and decolorize and deodorize. Decolorization and deodorization step of heating and stirring at 110 ~ 120 ℃, filtration step of removing acid clay added during purification by filtering the stirred and heated horse oil with a filter press, Antioxidant with respect to 87.8% by weight of stably purified Additive mixing step of adding 0.2% by weight of beeswax, 10% by weight of beeswax, and 2% by weight of perfume, respectively, and aging step (S32) of stabilizing physical properties by passing refined horse oil mixed with additives through a 150 mesh filter cloth and aging at room temperature It constitutes a method for manufacturing a cosmetic composition using refined horse oil.

Korean Registered Patent No. 10 - 0377262 (2003.03.26) Korean Registered Patent No. 10 - 0517465 (2005.09.28) Korean Registered Patent No. 10 - 1614500 (2016.04.21) Korean Registered Patent No. 10 - 0530215 (2005.11.22)

Horse oil is oil extracted from horse fat through a refining and aging process. Horse oil in its raw state contains various impurities and must be used through a predetermined refining process due to its unstable physical properties.

However, the method for refining horse oil to which the prior art is applied as described above is composed of a series of processes of refining from the fat of slaughtered horses by using a large amount of various organic solvents and synthetic additives, so that harmful substances to the human body are contained or nutritional components of horse oil are contained. There are issues with this loss.

In addition, horse oil is a natural oil that most closely resembles human sebum and has high penetration into the skin, and has a main effect on moisturizing and protecting the skin. It is not easy to use as a raw material for In addition, it is difficult to apply a large amount due to the oily feeling of use, so it is difficult to expect a significant effect on allergies or dermatitis.

Accordingly, the present invention was invented to solve the problems of the prior art as described above,

Process the horse fat dough with purified water at a constant temperature to obtain a horse oil extract in which the water layer and foreign substances are purified (S10);

Obtaining a powdery flavonoid extract by mixing 10 g of bee pollen extract and 10 g of herbal extract consisting of gardenia, pomegranate, and clove (S20, S30);

A step (S40) of mixing 1000 g of the horse oil extract and 20 g of the flavonoid extract and putting it into a wet nano grinder and pulverizing it into nanoparticles at 25 ° C. for 2 hours to obtain a composite extract (S40) Contains a large amount of active ingredients of horse oil and flavonoids Thus, it is possible to achieve the purpose of producing a complex extract having excellent efficacy in skin health and beauty.

The present invention is made to provide a complex extract prepared by effectively extracting and composing horse oil and flavonoids containing a large amount of various ingredients beneficial to the skin.

The present invention has the advantage of being harmless to the human body and safe for use on sensitive skin by excluding the use of various organic solvents or synthetic auxiliary agents and extracting horse oil extract using natural raw materials.

In particular, the present invention derives remarkable effects such as skin cell protection, anti-inflammation, wrinkle improvement, moisturizing, anti-allergy, atopic dermatitis, etc. by preparing a complex extract containing a flavonoid extract composed of horse oil extract and vegetable natural raw materials, and a more pleasant feeling of use and It promotes the effect of providing a fragrance to be used as a raw material for natural cosmetic compositions having skin beauty as well as skin health and protective effects.

1 to 2 is a schematic process flow diagram of the method for producing a horse oil flavonoid complex extract according to the present invention.
3 to 14 are tables and graphs of experimental results according to Experimental Examples 1 to 6 using the main raw material used in the manufacturing method of the horse oil flavonoid complex extract of the present invention.

Hereinafter, the composition and action according to a preferred embodiment of the manufacturing method of the horse oil flavonoid complex extract of the present invention will be described in detail with reference to the accompanying drawings. In the following description, detailed descriptions of parts that can be easily implemented by those skilled in the art may be omitted. In addition, since the following description describes the present invention with respect to preferred embodiments, the present invention is not limited by the following examples, and it is natural that various modifications may be provided without departing from the scope of the present invention. will be.

The manufacturing method of horse oil flavonoid complex extract to which the technology of the present invention is applied is made to effectively extract and compose horse oil and flavonoids containing a large amount of various ingredients beneficial to the skin to produce a complex extract, thereby improving skin cell protection, anti-inflammation, and wrinkle improvement. And it is noted that it relates to a technique for deriving a cosmetic effect and using it as a raw material for a natural cosmetic composition.

For this purpose, the method for preparing the horse oil flavonoid complex extract of the present invention, as shown schematically in FIGS. 1 and 2, goes through the steps (S10 to S30) of obtaining the main raw material, horse oil extract and flavonoid extract, respectively, and then mixing them to obtain a composite extract It consists of a series of steps to obtain (S40), specifically as follows.

Obtaining a horse oil extract, which is one of the main raw materials of the present invention (S10), is a step of obtaining a horse oil extract in which the water layer and foreign substances are purified by treating the horse fat dough with purified water at a certain temperature.

Horse oil contains various unsaturated fatty acids, as well as vitamins B1, B2, B6, and B12, which are effective in preventing wrinkles and improving blood circulation by protecting skin cell membranes from ultraviolet rays or aging and moisturizing deeply in the skin, and tocopherol, an antioxidant.

In order to use horse oil as a cosmetic, a predetermined purification process must be performed, and the step of obtaining the horse oil extract according to the present invention (S10) includes a bioremediation step (S11), a low-temperature treatment step (S12), and a high-temperature extraction step ( S13), the first separation step (S14), the second separation step (S15), and the horse oil extract obtaining step (S16) to obtain the horse oil extract.

The bio-cleaning step (S11) is a step of removing blood and foreign substances by immersing the horse fat dough in water.

The biological purification step (S11) excludes the use of various organic solvents or synthetic aids as in the prior art from the adipose tissue of the slaughtered horse, and removes blood and foreign substances that can be physically removed by immersion in water at room temperature. Configured to purify .

The low-temperature treatment step (S12) is a step in which 1 L of purified water is added to 1000 g of purified dough and treated at 40 to 50 ° C. for 1 hour.

In the low-temperature treatment step (S12), the purified dough is treated by immersion in water at 40 to 50 ° C., which is slightly higher than room temperature, to separate water while relaxing adipose tissue. When the temperature of the low-temperature treatment step (S12) exceeds the above range, the tissue is solidified, and moisture is not effectively separated along with excessive loss of the holding layer.

The high-temperature extraction step (S13) is a step of removing the water layer separated from the lower layer after the low-temperature treatment step (S12), and extracting at 80 ° C. for 3 hours by adding 1 L of purified water.

The high-temperature extraction step (S13) is configured to extract at a high temperature for a long time compared to the low-temperature treatment step (S12) to form the physical properties of the low-temperature processed adipose tissue into an oil phase. When the temperature of the high-temperature extraction step (S13) exceeds the above range, adipose tissue may be denatured or active ingredients may be lost.

The first separation step (S14) is a step of removing solids by filtering through a filter after the high-temperature extraction step (S13), and removing the water layer and foreign substances separated from the lower part of the oil layer.

In the primary separation step (S14), solids, water layers, and foreign substances generated during the extraction of adipose tissue at high temperature are primarily removed from the oil layer.

The second separation step (S15) is a step of injecting 0.5 L of purified water or cosmetic water into the oil layer that has passed through the first separation step (S14) and centrifuging at 2500 rpm for 20 minutes in a centrifuge.

In the second separation step (S15), the water layer and foreign substances contained in the oil layer that were not separated in the first separation step (S14) are centrifuged to ensure physical purity of the oil layer.

The horse oil extract obtaining step (S16) is a step of obtaining a horse oil extract by removing the water layer and foreign substances separated after the secondary separation step (S15) and storing at 2 to 4 ° C.

In the horse oil extract obtaining step (S16), the horse oil extract is mixed with a flavonoid extract to be described later and stored at a low temperature until used as a raw material for a cosmetic composition to form physical properties suitable for external use on the skin while maintaining the efficacy of the active ingredient. do.

On the other hand, the step of obtaining a flavonoid extract, which is another one of the main raw materials of the present invention (S20, S30), is a step of obtaining a flavonoid extract by configuring to mix the bee pollen extract and the herbal extract.

Bee pollen, which is a raw material of the bee pollen extract constituting the flavonoid extract, is particles mixed with saliva and parotene secreted from the molars of bees in the process of collecting pollen from plant flowers. Pollen in the air causes an allergic reaction, but ingestion of bee pollen is known to be effective in preventing allergies.

The bee pollen extract was subjected to a nano-grinding step (S21) of adding 300 g of bee pollen powder and 3 L of purified water to a wet nano-pulverizer and grinding into nanoparticles at 25 ° C. for 5 hours, and a nano-grinding step (S21), followed by freeze-drying It is prepared by a bee pollen extract obtaining step (S22) of powdering and storing at 2 ~ 4 ° C to obtain a bee pollen extract.

Since the bee pollen is surrounded by a hard outer shell, it is not easily decomposed by digestive enzymes of most animals or insects and acids and alkalis. When bee pollen is exposed to a solution, some active ingredients are eluted through germpores by osmosis, but the efficiency is very low, so the efficacy is very limited when applied externally to the skin.

Therefore, the shell of the bee pollen is ultra-finely pulverized through the step of pulverizing the bee pollen into nanoparticles by a wet nano-pulverizer together with purified water.

In addition, the ultrafinely pulverized bee pollen as described above is lyophilized to use both the hull and the active ingredient to obtain a powdered bee pollen extract, which is a raw material of the flavonoid extract.

Gardenia, which is a raw material of the herbal extract, has blood pressure lowering, antibacterial, and anti-inflammatory properties and is widely used as a medicinal material. Pomegranate is effective for the skin because it contains a large amount of natural plant estrogens and vitamins, and cloves are known to have antioxidant, antibacterial, and anti-inflammatory properties.

The oriental medicine extract was extracted by adding 3L of 50 to 80% ethanol solution to 100g of gardenia, 100g of pomegranate, and 100g of cloves and extracting at 50 ° C for 5 hours (S31) and extracting raw materials (S31). Aging step of aging at room temperature for 10 hours (S32), aging step (S32), filtering with a filter and evaporating ethanol to remove the solvent (S33), and solvent removal step (S33), followed by freeze-drying It is configured to be prepared by powdering and storing at 2 ~ 4 ℃ to obtain an herbal extract step (S34) to obtain an herbal extract.

The herbal extract is also powdered by lyophilization in a state where the solvent is removed after extraction and aging to maximize the active ingredients of gardenia, pomegranate, and clove to obtain the herbal extract, which is a secondary raw material of the flavonoid extract.

Therefore, a powdery flavonoid extract was obtained by mixing 10 g of the bee pollen extract prepared as described above and 10 g of an herbal extract composed of gardenia, pomegranate, and clove.

The step of obtaining the composite extract (S40) is a step of obtaining a composite extract by mixing 1000 g of the horse oil extract and 20 g of the flavonoid extract and pulverizing them into nanoparticles at 25 ° C. for 2 hours by putting them in a wet nano grinder.

Since the flavonoid extract is introduced in a freeze-dried powder state, in the step of obtaining the composite extract (S40), the horse oil extract and the flavonoid extract are mixed and effectively dissolved in the oil layer through an ultra-fine grinding process, and then used as a raw material for a cosmetic composition. The complex extract is stored at a low temperature until it is configured to form physical properties suitable for external use on the skin while maintaining the efficacy of the active ingredient.

After the step of obtaining the composite extract (S40), a process of expressing various colors by adding natural pigments and natural materials to the composite extract may be further performed.

The composite extract prepared by a series of steps as described above is configured to be used as a raw material for a cosmetic composition having a formulation selected from the group consisting of skin, lotion, essence, gel, cream, pack, soap, and cleanser.

<Experimental Example 1>

Efficacy confirmation experiment through component analysis of horse oil extract, which is one of the main raw materials used in the manufacturing method of horse oil flavonoid complex extract according to the present invention.

In order to confirm the efficacy of the horse oil extract, the flavonoid extract was analyzed for the fatty acid components of the horse oil extract in an unmixed state. As a result, as shown in Figures 3 to 4, palmitoleic acid (Omega -7) and unsaturated fatty acids such as oleic acid (omega-9), linoleic acid (omega-6), and linolenic acid (omega-3), which have atopy and skin trouble improvement and moisturizing effects, were confirmed to be very high.

<Experimental Example 2>

Cell protection efficacy test of flavonoid extract consisting of bee pollen extract and herbal extract according to the present invention.

1) Cytotoxicity of the flavonoid extract was evaluated by LDH release assay method using CCD-986sk cells. As a result, as shown in the graph of FIG. 5 below, the LDH efflux of the flavonoid extract was significantly reduced compared to the control group, and it was confirmed that there was no toxicity to cells and a cell protective effect.

2) The effect of flavonoid extract on heme oxygenase (HO)-1 mRNA expression, an antioxidant protein, was evaluated in HEKa cells. As a result, as shown in the graph of FIG. 6 below, it was confirmed that the flavonoid extract increased (HO)-1 mRNA by about 10 times or more compared to the control group. Therefore, it was confirmed that the flavonoid extract had a cell protective effect without adversely affecting cell viability and was involved in the expression of antioxidant proteins.

<Experimental Example 3>

Anti-inflammatory efficacy test of flavonoid extract consisting of bee pollen extract and herbal extract according to the present invention.

1) A simulation using LPS (Lipopolysaccharide) was conducted to confirm the inhibitory effect of NO (Nitric oxide) production of the flavonoid extract. As a result, as shown in the graph of FIG. 7 below, it was confirmed that the NO production inhibitory effect of the flavonoid extract was very good.

2) In Raw 264.7 cells, the inhibitory effect of iNOS (inducible nitric oxide synthase) expression by LPS of the flavonoid extract was measured. As a result, as shown in FIG. 8 below, it was confirmed that the flavonoid extract effectively inhibited the expression of iNOS.

<Experimental Example 4>

Antioxidant and anti-wrinkle efficacy test of flavonoid extract consisting of bee pollen extract and oriental medicine extract according to the present invention.

1) As a result of treating the herbal extract in fibroblasts in order to evaluate the inhibitory effect of the flavonoid extract on the amount of collagenase mRNA expression, it was confirmed that collagenase mRNA was significantly reduced compared to the control group as shown in graph A of FIG. 9 . As a result of the combined treatment of bee pollen extract and herbal extract, as shown in graph B of FIG.

2) The expression level of collagenase protein was analyzed using the ELISA measurement method with reference to the evaluation results of the efficacy of inhibiting collagenase mRNA expression level. As a result, as shown in the graph of FIG. 10, it was confirmed that collagenase protein synthesis was significantly inhibited in the flavonoid extract compared to the control group.

<Experimental Example 5>

Moisturizing efficacy test of flavonoid extract consisting of bee pollen extract and herbal extract according to the present invention.

The effect of moisturizing factor expression in keratinocytes and the quantitative evaluation of hyaluronan protein were conducted to verify the moisturizing effect of the pollen complex extract. As a result of evaluating the expression of aquaporin3 (AQP3) mRNA in human epidermal keratinocytes adult (HEKa) cells, it was confirmed that the flavonoid extract increased moisturizing factor expression compared to the control group, as shown in the graph of FIG. 11 .

<Experimental Example 6>

Anti-allergic efficacy test of flavonoid extract consisting of bee pollen extract and herbal extract according to the present invention.

1) As a result of confirming the lipoxygenase inhibitory effect to evaluate the anti-allergic efficacy of the flavonoid extract, it was confirmed that the flavonoid extract suppresses the allergic reaction compared to the control group (NDGA), as shown in graphs A and B of FIG. 12.

2) In order to evaluate the anti-allergic efficacy of the flavonoid extract, RBL-2H3 cells were used to induce allergy by IgE and DNP stimulation, and then the effect on β-Hexosaminidase secretion was confirmed. Flavonoids as shown in graphs A and B of FIG. 13 below It was confirmed that the extract showed a significant degranulation inhibitory effect compared to the control group (Ketotifen).

3) In order to evaluate the anti-allergic efficacy of flavonoid extracts, RBL-2H3 cells were used to confirm the IL-4 and IL-13 gene expression levels of Th2 cytokines induced after allergy by IgE and DNP stimulation by RT-PCR. As shown in graphs A and B of FIG. 14, it was confirmed that the cytokine expression of the flavonoid extract was significantly reduced compared to the control group (Con).

Horse oil flavonoid composite extract manufacturing method according to the present invention as described above is made to effectively extract and compose horse oil and flavonoids to prepare a composite extract having various efficacies.

The present invention has the advantage of being harmless to the human body and safe for use on sensitive skin by excluding the use of various organic solvents or synthetic auxiliary agents as in the prior art and extracting horse oil extract using natural raw materials.

In particular, the present invention derives remarkable effects such as skin cell protection, anti-inflammation, wrinkle improvement, moisturizing, anti-allergy, etc. by preparing a complex extract containing a flavonoid extract composed of horse oil extract and vegetable natural raw materials, as well as skin beauty, skin health and It has the effect of being used as a raw material of a cosmetic composition having a protective effect.

S10: obtaining horse oil extract
S20, S30: obtaining a flavonoid extract
S40: step of obtaining a composite extract
S11: Bioremediation step S12: Low temperature treatment step
S13: high-temperature extraction step S14: first separation step
S15: Secondary Separation Step S16: Horse Oil Extract Obtaining Step
S21: Nano grinding step S22: Bee pollen extract obtaining step
S31: raw material extraction step S32: aging step
S33: solvent removal step S34: herbal extract obtaining step

Claims (5)

Process the horse fat dough with purified water at a constant temperature to obtain a horse oil extract in which the water layer and foreign substances are purified (S10);
Obtaining a powdery flavonoid extract by mixing 10 g of bee pollen extract and 10 g of herbal extract consisting of gardenia, pomegranate, and clove (S20, S30);
A step (S40) of mixing 1000 g of the horse oil extract and 20 g of the flavonoid extract and putting them in a wet nano grinder to grind them into nanoparticles at 25 ° C. for 2 hours to obtain a composite extract,
Obtaining the horse oil extract (S10),
A biological purification step (S11) of soaking the horse fat dough to remove blood and foreign substances;
A low-temperature treatment step (S12) in which 1 L of purified water is added to 1000 g of purified dough and treated at 40 to 50 ° C for 1 hour to separate moisture while relaxing adipose tissue;
After the low-temperature treatment step (S12), a high-temperature extraction step (S13) of removing the layer-separated water layer in the lower layer to form the physical properties of the adipose tissue into an oil phase, and injecting 1 L of purified water and extracting at 80 ° C. for 3 hours and,
After the high-temperature extraction step (S13), the solids are removed by filtering with a filter, and the first separation step (S14) of separating the oil layer by removing the water layer and foreign substances separated from the lower layer of the oil layer;
0.5L of purified water or cosmetic water is injected into the oil layer that has passed through the first separation step (S14), and centrifuged at 2500 rpm for 20 minutes in a centrifuge to separate the water layer and foreign substances contained in the oil layer to secure purity. (S15) and,
A horse oil extract obtaining step (S16) of removing the water layer and foreign substances separated after the second separation step (S15) and storing at 2 to 4 ° C to obtain a horse oil extract,
In the step of obtaining the powdery flavonoid extract (S30), the herbal extract,
A raw material extraction step (S31) of adding 3 L of 50 to 80% ethanol solution to 100 g of gardenia, 100 g of pomegranate, and 100 g of cloves and extracting at 50 ° C for 5 hours;
A ripening step (S32) of aging at room temperature for 10 hours after the raw material extraction step (S31);
After passing through the aging step (S32), a solvent removal step (S33) of filtering with a filter and removing ethanol by evaporation;
After the solvent removal step (S33), it is lyophilized to powder and stored at 2 ~ 4 ° C. to obtain an herbal extract obtained step (S34) to obtain a horse oil flavonoid complex extract manufacturing method. delete delete delete In the horse oil flavonoid complex extract prepared according to claim 1,
The horse oil flavonoid complex extract,
Consisting of horse oil extract obtained from horse fat dough, bee pollen extract, and powdery flavonoid extract obtained from herbal extracts consisting of gardenia, pomegranate, and clove as raw materials,
The horse oil flavonoid complex extract is characterized in that it is a raw material for a natural cosmetic composition for skin health and protection having a formulation selected from the group consisting of skin, lotion, essence, gel, cream, pack, soap, cleanser.

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