CN102860507B - Novel grape seed extract and preparation method thereof - Google Patents
Novel grape seed extract and preparation method thereof Download PDFInfo
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- CN102860507B CN102860507B CN201210361095.0A CN201210361095A CN102860507B CN 102860507 B CN102860507 B CN 102860507B CN 201210361095 A CN201210361095 A CN 201210361095A CN 102860507 B CN102860507 B CN 102860507B
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- grape seed
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Abstract
The invention relates to novel grape seed extract and a preparation method thereof. Each part of the grape seed extract is made from 5-10 parts of grape seed by weight. The grape seed extract comprises volatile oil, liposoluble constituent and solid dry powder. The preparation method includes the steps of SEF-CO2 (supercritical CO2) extraction and ethanol extraction. The grape seed, black bean and bittersweet herb can also be used as raw materials to prepare the grape seed extract, and the grape seed extract with vitamin E, PEG400 (polyethylene glycol 400), glycerin and tween 80 is made into soft capsules. The grape seed extract fully retains effective constituents in plant materials, and is suitable for general population. 300 cases of use by different people show that average functional effective rate reaches more than 94.5%.
Description
Technical field
The invention belongs to field of health care products, particularly a kind of Novel grape seed extract and method for making thereof.
Background technology
Grape seed extract is rich in OPC OPC, and OPC is the most efficient antioxidant and the free radical scavenger of the plant origin found so far.Grape pip is the best beautifying and anti-aging nutrient supplement food that generally acknowledge in Europe, has the good reputation of " skin vitamin " " oral cosmetics ".There is unique moist skin, whitening, functions of removing chloasma effect.
The preparation method of grape seed extract has multiple, as: the employing water extraction taking CN00134314.9 as representative, alcohol precipitation traditional handicraft; Organic solvent taking CN200710191255.0 as representative extracts, then by the refining technique of macroreticular resin; Superfine communication technique taking CN200610104864.3 as representative; The organic solvent of the inorganic ore deposit acid taking CN03137933.8 as representative extracts, inorganic rice bran; With the process for biological enzyme extraction of grape seed polypeptide protein of CN200410011197.5; Employing supercritical technology taking CN02144708.X, CN200710153807.9 as representative.
Because the material composition of plant extracts can vary widely according to preparation technology's difference, thereby above-mentioned technology obtains the each tool advantage of grape seed extract and deficiency, but all there is its unique value, therefore the method for grape seed extract is continued to improve and still have realistic meaning, in addition, the compound that existing grape seed extract and other plant extract are made is less, mainly comprises: the grape seed extract of CN200910018841.4, soybean oil extract, vitamin E; CN200910041097.X grape seed extract 120g, pearl powder 50g, vitamin e1 0g, beeswax 10g, soybean oils 310 etc., find plant material performance combination advantage people's the effort target being especially used in conjunction with grape pip.It is more than the technical problem that existing grape seed extract field exists.
Summary of the invention
The invention provides a kind of brand-new grape seed extract and its method for making is provided, simultaneously, the invention provides grape seed extract and method for making that plant materials such as comprising grape pip, black soya bean, bittersweet is made, particularly the invention provides a kind of soft capsule and method for making that grape seed extract is made of applying.Describe in detail realizing the object of the invention technical scheme below.
A kind of Novel grape seed extract of the present invention, the weight portion of preparing the raw material of described grape seed extract is: grape pip 5-100 black soya bean 5-100 bittersweet 5-100
Preparing described grape seed extract step is: get grape pip, black soya bean, bittersweet and put SFE-CO
2in extractor, 50 DEG C of extraction temperature, resolve pressure 6-8Mpa, resolution temperature 35-40 DEG C, extracting pressure 24Mpa, extraction time 2 hours, obtain volatile oil and liposoluble constituent;
By SFE-CO
2extract the dregs of a decoction meal after volatile oil and liposoluble constituent, added octuple amount 80% ethanol room temperature dipping 24 hours, filtered, collected liquid; The dregs of a decoction are added to 8 times of amount 80% ethanol room temperatures again and flood 24 hours, filter, abandon the dregs of a decoction, after liquid merges, reduced pressure concentration under the condition of 0.07MPa, reclaims ethanol, and spraying is dried to obtain solids dry powder.
The weight portion of the raw material of preferred grape seed extract is: grape pip 30, black soya bean 30, bittersweet 30.
Plant material of the present invention source is:
Grape pip is the seed of Vitis vitaceae grape.
Black soya bean is the black seed of legume soybean Glycinemax (L.) merr.
Bittersweet is the herb of plant of Solanaceae bittersweet Solanum lyratumThunb..
Above-mentioned grape seed extract of the present invention all can be made into health products, the oral solid formulation that adds suitable excipient to make by grape seed extract and other active components.
Preferably vitamin E of the present invention's " his active component ", the weight ratio that preferred ratio is grape seed extract and vitamin E is 10-100:1.
Optimum oral formulations is for making soft capsule, and the excipient of preparing soft capsule comprises PEG400, glycerine, Tween 80, each composition weight portion composition:
Grape seed extract 10-100 vitamin E 0.5-1
PEG400100-300 glycerine 10-30 Tween 80 10-30.
In grape seed extract, volatile oil, liposoluble constituent, solids dry powder three weight ratio are 0.1-0.5:5-10:50-200.
Preferred each composition weight portion consists of:
Grape seed extract 50 vitamin Es 0.973
PEG400200 glycerine 20 Tween 80s 20.
The method for making of soft capsule of the present invention is, volatile oil, fat-soluble extract, Tween 80 are mixed, and adds the PEG400 that is approximately equivalent to volatile oil 1/2 amount, mixes, and obtains volatile oil emulsion; Separately get glycerine, remaining PEG400, solids dry powder and remaining water, mix, by homogenization machine homogenize 30 minutes, then add volatile oil emulsion to mix, by homogenization machine homogenize 30 minutes, to obtain final product.
Beneficial effect of the present invention is, inventor is by volatile oil more in each plant material and liposoluble constituent SFE-CO
2extract, simultaneously can not be by SFE-CO
2the polarity composition extracting dissolves and extracts with ethanol.The grape seed extract making, has fully retained contained active ingredient in plant material, and suitable crowd crowd is extensive, comprising: hope delays senility, the person of retaining youth; Skin splash, pitch-dark, has whitening demander; Adult female's (showing as irregular menstruation) of estrogen level imbalance; Patients with Cardiovascular/Cerebrovascular Diseases supplemental treatment; Wish to keep the female middle-aged/climacteric women of better sexual life.By the use of the above different crowd of 300 example, more function average effectiveness level reaches more than 94.5%, and concrete feature is as follows:
1, superpower anti-oxidant, remove free radical, delay senility.
2, reduce dermal melanin calmness, freckle removing and whitening.
3, alleviate menopause symptom, as flush, hectic fever, emotional lability, vaginal dryness etc.
4, maintain skin elasticity, avoid wrinkle to produce too early, delay skin aging.
5, protection cardiovascular and cerebrovascular, prevention of stroke, the spastic headache of alleviating vascular, dizzy.
Below to typical application example explanation in 300 routine application crowds:
Typical case's application
Clock, female, 25 years old, long-time tired, insomnia forgetfulness, skin splash showed increased, pitch-dark, takes 3 years grape seed extract soft capsules of the present invention (specification is 500mg × 60/bottle), 2 of every days continuously, the good results are evident, and skin is not only thin but also white, irritated.
Luo, female, 39 years old, irregular menstruation over the past half year, looked wane and sallow, after diagnosing for due to estrogen level imbalance, oral grape seed extract soft capsule of the present invention (specification is 500mg × 60/bottle), two of every days, after 1 month, menstruation recovery is normal, continue to take half a year, obviously feel that energetic, skin is fine and smooth glossy.
Answer certain, man, 47 years old, suffer from coronary heart disease etc. at interior cardiovascular and cerebrovascular disease for many years, attempt multi-medicament effect undesirable, before half a year, beginning is taken grape seed extract soft capsule (500mg × 60/bottle) as supplemental treatment simultaneously, result vascular health is unimpeded, improve blood circulation, blood test indices all recovers normally, still takes adhering at present.
Gold certain, female, 49 years old, menopause symptom highly significant, took grape seed extract soft capsule (500mgx60 grain/bottle) after 2 months, had obviously brought into play the effect of fatigue-relieving, enhancing energy, it is normal that sexual life also recovers.
Detailed description of the invention
Embodiment 1 grape seed extract
Get grape pip 500g and put SFE-CO
2in extractor, 40 DEG C of extraction temperature, resolve pressure 6-8Mpa, 40 DEG C of resolution temperatures, extracting pressure 30Mpa, extraction time 3 hours, obtain volatile oil and liposoluble constituent;
By SFE-CO
2extract the dregs of a decoction meal after volatile oil and liposoluble constituent, added 9 times of amounts, 70% ethanol room temperature dipping 12 hours, filtered, collected liquid; The dregs of a decoction are added to 9 times of amount 70% ethanol room temperatures again and flood 12 hours, filter, abandon the dregs of a decoction, after liquid merges, reduced pressure concentration under the condition of 0.07MPa, reclaims ethanol, and spraying is dried to obtain solids dry powder.
Make total grape seed extract 48.7g, wherein volatile oil 0.7g, liposoluble constituent 5g, solids dry powder 43g.
Embodiment 2 grape seed extracts
Grape pip 500g, black soya bean 500g, bittersweet 500g put SFE-CO
2in extractor, 50 DEG C of extraction temperature, resolve pressure 6-8Mpa, resolution temperature 35-40 DEG C, extracting pressure 24Mpa, extraction time 2 hours, obtain volatile oil and liposoluble constituent;
By SFE-CO
2extract the dregs of a decoction meal after volatile oil and liposoluble constituent, added octuple amount 80% ethanol room temperature dipping 24 hours, filtered, collected liquid; The dregs of a decoction are added to 8 times of amount 80% ethanol room temperatures again and flood 24 hours, filter, abandon the dregs of a decoction, after liquid merges, reduced pressure concentration under the condition of 0.07MPa, reclaims ethanol, and spraying is dried to obtain solids dry powder.
Make total grape seed extract 158.3g, wherein volatile oil 3.3g, liposoluble constituent 17g, solids dry powder 138g.
Embodiment 3 grape seed extract soft capsules
Grape seed extract (making by embodiment 1 method) 2.5kg vitamin E 97.3g
PEG4005kg glycerine 1kg Tween 80 1kg.
Volatile oil, fat-soluble extract, Tween 80 are mixed, add the PEG400 that is approximately equivalent to volatile oil 1/2 amount, mix, obtain volatile oil emulsion; Separately get glycerine, remaining PEG400, solids dry powder and remaining water, mix, by homogenization machine homogenize 30 minutes, then add volatile oil emulsion to mix, by homogenization machine homogenize 30 minutes, to obtain final product.
The every 100g of soft capsule content contains: OPC 16g, vitamin E 0.973g.
Embodiment 4 grape seed extract soft capsules
Grape seed extract (making by embodiment 2 methods) 2.5kg vitamin E 97.3g
PEG4005kg glycerine 1kg Tween 80 1kg.
Volatile oil, fat-soluble extract, Tween 80 are mixed, add the PEG400 that is approximately equivalent to volatile oil 1/2 amount, mix, obtain volatile oil emulsion; Separately get glycerine, remaining PEG400, solids dry powder and remaining water, mix, by homogenization machine homogenize 30 minutes, then add volatile oil emulsion to mix, by homogenization machine homogenize 30 minutes, to obtain final product.The every 100g of soft capsule content contains: OPC 16g, daidzin 10g, anthocyanin 3g, vitamin E 0.973g.
The above embodiment is only that the preferred embodiment of the present invention is described; not scope of the present invention is limited; design under the prerequisite of spirit not departing from the present invention; various distortion and improvement that those of ordinary skill in the art make technical scheme of the present invention, all should fall in the definite protection domain of claims of the present invention.
Claims (3)
1. the health products that grape seed extract makes, it is characterized in that, described health products are the soft capsules that add suitable excipient to make by described grape seed extract and vitamin E, and described excipient comprises PEG400, glycerine, Tween 80, and the each composition weight portion of described soft capsule consists of:
Grape seed extract 10-100 vitamin E 0.5-1
PEG400 100-300 glycerine 10-30 Tween 80 10-30
Described grape seed extract comprises volatile oil, liposoluble constituent, solids dry powder;
The parts by weight of raw materials of preparing described grape seed extract is: grape pip 5-100 black soya bean 5-100 bittersweet 5-100;
Preparing described grape seed extract step is: get grape pip, black soya bean, bittersweet and put SFE-CO
2in extractor, 50 DEG C of extraction temperature, resolve pressure 6-8Mpa, resolution temperature 35-40 DEG C, extracting pressure 24Mpa, extraction time 2 hours, obtain volatile oil and liposoluble constituent;
By SFE-CO
2extract the dregs of a decoction meal after volatile oil and liposoluble constituent, added octuple amount 80% ethanol room temperature dipping 24 hours, filtered, collected liquid; The dregs of a decoction are added to 8 times of amount 80% ethanol room temperatures again and flood 24 hours, filter, abandon the dregs of a decoction, after liquid merges, reduced pressure concentration under the condition of 0.07MPa, reclaims ethanol, and spraying is dried to obtain solids dry powder;
The method for making of described soft capsule is: described volatile oil, fat-soluble extract, Tween 80 are mixed, add the PEG400 that is equivalent to volatile oil 1/2 amount, mix, obtain volatile oil emulsion; Separately get glycerine, remaining PEG400, solids dry powder and water, mix, by homogenization machine homogenize 30 minutes, then add volatile oil emulsion to mix, by homogenization machine homogenize 30 minutes, to obtain final product.
2. health products as claimed in claim 1, is characterized in that, the parts by weight of raw materials of preparing described grape seed extract is:
Grape pip 30 black soya bean 30 bittersweets 30.
3. arbitrary health products as claimed in claim 1 or 2, is characterized in that, each composition weight portion consists of:
Grape seed extract 50 vitamin Es 0.973
PEG400 200 glycerine 20 Tween 80s 20.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210361095.0A CN102860507B (en) | 2012-09-25 | 2012-09-25 | Novel grape seed extract and preparation method thereof |
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| CN201210361095.0A CN102860507B (en) | 2012-09-25 | 2012-09-25 | Novel grape seed extract and preparation method thereof |
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| CN102860507A CN102860507A (en) | 2013-01-09 |
| CN102860507B true CN102860507B (en) | 2014-10-29 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104543122A (en) * | 2014-12-16 | 2015-04-29 | 新昌县小将镇乌牛岗家庭农场 | Tea with effects of maintaining beauty and keeping young |
| CN106071920A (en) * | 2016-05-30 | 2016-11-09 | 射洪县俊成食品有限公司 | A kind of instant lucid ganoderma pig gristle and preparation method thereof |
Citations (2)
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|---|---|---|---|---|
| CN1724531A (en) * | 2005-07-18 | 2006-01-25 | 天津大学 | Method of extracting proto anthocyanidin by supercritical extraction grape seed oil and resin absorption |
| CN101386806A (en) * | 2007-09-11 | 2009-03-18 | 王芳 | Supercritical CO2 extraction of grape-kernel oil and method for extracting oligoprotoanthocyanidin by solvent process |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100389676C (en) * | 2004-09-24 | 2008-05-28 | 江西本草天工科技有限责任公司 | Health-care food and its soft capsule for improving immunity of anti alimentary canal infection, and its prepn. method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724531A (en) * | 2005-07-18 | 2006-01-25 | 天津大学 | Method of extracting proto anthocyanidin by supercritical extraction grape seed oil and resin absorption |
| CN101386806A (en) * | 2007-09-11 | 2009-03-18 | 王芳 | Supercritical CO2 extraction of grape-kernel oil and method for extracting oligoprotoanthocyanidin by solvent process |
Non-Patent Citations (4)
| Title |
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| 中药软胶囊制备研究概述;张楚晗等;《第三届中国中药商品学术年会暨首届中药葛根国际产业发展研讨会论文集》;20120715;第294-302页 * |
| 张楚晗等.中药软胶囊制备研究概述.《第三届中国中药商品学术年会暨首届中药葛根国际产业发展研讨会论文集》.2012, |
| 白英提取物的抗氧化作用研究;谢永芳等;《时珍国医国药》;20061231;第17卷(第6期);第899-890页 * |
| 谢永芳等.白英提取物的抗氧化作用研究.《时珍国医国药》.2006,第17卷(第6期), |
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Effective date of registration: 20170216 Address after: 102200 Beijing city small town east of Changping District Beach Village Street No. 394 B block comprehensive commercial building 12 building 4 layer 1220 Patentee after: Synergy hi tech (Beijing) Pharmaceutical Technology Co., Ltd. Address before: 100026 Chaoyang District Chaoyang Road, Beijing, building 1, No. 2-0301, No. 69 Patentee before: Beijing Kangjian Renren Electronic Business Co.,Ltd. |
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Granted publication date: 20141029 Termination date: 20170925 |
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