CN100352362C - Earthworm active peptide antibacerial feed material and its production method - Google Patents
Earthworm active peptide antibacerial feed material and its production method Download PDFInfo
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- CN100352362C CN100352362C CNB2003101155834A CN200310115583A CN100352362C CN 100352362 C CN100352362 C CN 100352362C CN B2003101155834 A CNB2003101155834 A CN B2003101155834A CN 200310115583 A CN200310115583 A CN 200310115583A CN 100352362 C CN100352362 C CN 100352362C
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- earthworm
- active peptide
- coelomic fluid
- peptide
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention relates to an earthworm active peptide antibacerial feed material used as a feed additive and a production method thereof. The present invention has the technical scheme that: the coelomic fluid drained from the earthworm is extracted by salt water to be prepared into earthworm peptide powder which is applied to the production of the earthworm active peptide antibacerial feed material; (1) the extraction of the coelomic fluid of the earthworm comprises the steps that the scarfskin of the earthworm is slightly brushed by the salt water, and the earthworm is stimulated by the salt water so as to drain the coelomic fluid which is collected; (2) the preparation of the earthworm peptide powder comprises the steps that the extracted coelomic fluid of the earthworm is mixed and stirred with the sterilized earthworm faeces or wheat brans and is aired for standby; because the coelomic fluid of the earthworm contains a great amount of antibacerial peptid, the mixture is named the earthworm peptide powder; (3) the earthworm peptide powder is added to the earthworm active peptide antibacerial feed material to be prepared into an earthworm active peptide antibacerial feed product. Because the coelomic fluid of the earthworm contains a great amount of antibacerial peptid, the disease resistance of the organism is improved. The earthworm active peptide antibacerial feed product of the present invention has the advantages of bacterium prevention and bacteriostasis, and has the efficiency of raising the immunizing power of animals, enhancing the appetite, helping the digestion, improving the food conversion ratio, reducing the production cost and improving the economic benefit. The present invention has the characteristics of reasonable technology, rapidness and convenience for extracting the coelomic fluid of the earthworm, high quality of the earthworm active peptide antibacerial feed material, bacterium prevention, bacteriostasis, etc.
Description
Affiliated technical field:
The present invention relates to a kind of earthworm active peptide antiseptic feed raw material and production method thereof, particularly earthworm active peptide antiseptic feed raw material and the production method of using as feed addictive thereof.
Background technology:
Because the metabolite (wormcast) of earthworm and earthworm contains very high protein and vitamin, nutritive value is very high, can be used as additive, substitute fish meal and wheat bran in the common feed, reduce cost, increase economic efficiency, " vermiculture new technology " book that the Sun Zhenjun of the China Agricultural University professor who published in 1993 is shown just has detailed argumentation.At present, people's earthworm active peptide antiseptic feed feedstock production process technological process commonly used is as follows: 1. wormcast ferments at a certain temperature, and general temperature is controlled at below 68 ℃, fermentation time 48 hours; 2. add the earthworm body by a certain percentage, for example the earthworm powder that earthworm is made; 3. continue fermentation 24 hours, temperature also is controlled at below 68 ℃; 4. dry; 5. be packaged into earthworm active peptide antiseptic feed raw produce.The earthworm active peptide antiseptic feed raw material made from this technology contains a large amount of microorganisms, comprise harmful bacterium such as Escherichia coli, Pseudomonas aeruginosa, influence the product quality of earthworm active peptide antiseptic feed raw material and the health of letting animals feed, bring hidden danger for the use of feed.
Summary of the invention:
The object of the invention provides a kind of earthworm active peptide antiseptic feed raw material and production method thereof, harmful bacterium in elimination and the minimizing earthworm active peptide antiseptic feed raw material, produce high-quality earthworm active peptide antiseptic feed raw produce, solve the problems referred to above that exist in the background technology.
Technical scheme of the present invention is: extract the coelomic fluid of earthworm discharging with salt solution, make earthworm peptide powder, add and produce in the earthworm active peptide antiseptic feed raw material, concrete technological process is as follows:
1. the extraction of coelomic fluid of earthworm is gently brushed the earthworm epidermis with salt solution, and earthworm is subjected to salt solution to be stimulated, and can discharge coelomic fluid, collects;
2. the making of earthworm peptide powder mixes stirring to wormcast or wheat bran after the coelomic fluid of earthworm that extracts and the sterilization, dries standbyly, and owing to the coelomic fluid of earthworm contains a large amount of antibacterial peptides, this mixture is called earthworm peptide powder;
3. earthworm peptide powder is added and produce in the earthworm active peptide antiseptic feed raw material, make earthworm active peptide antiseptic feed raw produce.
Because the coelomic fluid of earthworm contains a large amount of antibacterial peptides, can the direct killing bacterium, virus, kill and wound simultaneously and suppress harmful germ, can improve the human body resistance against diseases.The coelomic fluid that how to obtain earthworm easily and effectively becomes the key of this technology of enforcement, and the present invention stimulates earthworm to discharge coelomic fluid with salt solution, makes earthworm peptide powder, adds in the earthworm active peptide antiseptic feed raw material, and convenient and swift, effect is remarkable.
For more effective harmful bacterium of killing in the earthworm active peptide antiseptic feed raw material, can be with the wormcast high-temperature sterilization, temperature is controlled at 70-104 ℃, kills harmful bacterium in the wormcast with high temperature.
In order to keep activation of microorganism in the earthworm active peptide antiseptic feed raw material, can in earthworm active peptide antiseptic feed raw material, add earthworm liquid, earthworm liquid is that earthworm is placed in the water, adds brown sugar, makes the earthworm in the water be melted into liquid.
Can also in earthworm active peptide antiseptic feed raw material, add biological bacteria, ferment bacterium etc. for example, the absorption that helps digest improves autoimmunity.
The earthworm active peptide antiseptic feed raw produce that adopts the present invention to produce has antibiotic, bacteriostasis, improves animal immunizing power, improves a poor appetite, and helps digest, and improves food conversion ratio, and reduces production costs, and increases economic efficiency.
The present invention has characteristics such as technology is reasonable, the extraction coelomic fluid of earthworm is efficient and convenient, the earthworm active peptide antiseptic feed raw material quality height of production, antibacterial bacteriostatic.
The specific embodiment:
The invention will be further described by the following examples.
Embodiment one
The concrete technological process of present embodiment production earthworm active peptide antiseptic feed raw material is as follows:
1. the extraction of coelomic fluid of earthworm, earthworm is placed on the bottom to be had in the chest of filter cloth, is provided with container below the chest, gently brushes the earthworm epidermis with salt solution, the weight ratio of salt and water is 1: 3 in the salt solution, earthworm is subjected to salt solution to be stimulated, and can discharge coelomic fluid, sees through filter cloth, drip in the container and collect, earthworm just is upset and can come to harm, and can continue to raise, and coelomic fluid of earthworm is extracted in recycling;
2. the making of earthworm peptide powder, the coelomic fluid of earthworm that extracts is mixed stirring with wormcast after the sterilization, and the weight ratio between the wormcast after coelomic fluid of earthworm and the sterilization is 1: 2, dries standby, because the coelomic fluid of earthworm contains a large amount of antibacterial peptides, this mixture is called earthworm peptide powder;
3. wormcast fermentation, temperature is controlled at below 65 ℃, fermentation time 48 hours, and the weight of wormcast is calculated as 100%;
4. will produce earthworm active peptide antiseptic feed raw material raw material wormcast high temperature sterilization, temperature is controlled at 85 ℃, kills the harmful bacterium in the wormcast, increases beneficial bacterium;
5. earthworm peptide powder is added and produce in the earthworm active peptide antiseptic feed raw material raw material, stir, the addition of earthworm peptide powder is 3% of a wormcast, percentage by weight;
6. dry, control moisture≤13% is packaged into earthworm active peptide antiseptic feed raw produce.
Embodiment two
The concrete technological process of present embodiment production earthworm active peptide antiseptic feed raw material is as follows:
1. the extraction of coelomic fluid of earthworm, earthworm is placed on the bottom to be had in the chest of filter cloth, is provided with container below the chest, gently brushes the earthworm epidermis with salt solution, the weight ratio of salt and water is 1: 4 in the salt solution, earthworm is subjected to salt solution to be stimulated, and can discharge coelomic fluid, sees through filter cloth, drip in the container and collect, earthworm just is upset and can come to harm, and can continue to raise, and coelomic fluid of earthworm is extracted in recycling;
2. the making of earthworm peptide powder mixes stirring to the coelomic fluid of earthworm that extracts with wheat bran, the weight ratio between coelomic fluid of earthworm and the wheat bran is 1: 2, dries standbyly, and owing to the coelomic fluid of earthworm contains a large amount of antibacterial peptides, this mixture is called earthworm peptide powder;
3. wormcast fermentation, temperature is controlled at below 65 ℃, fermentation time 48 hours, and the weight of wormcast is calculated as 100%;
4. will produce earthworm active peptide antiseptic feed raw material raw material wormcast high temperature sterilization, temperature is controlled at 90 ℃, kills the harmful bacterium in the wormcast, increases beneficial bacterium;
5. wheat bran or rice chaff and ferment bacterium being added water stirs, add in the raw material of producing earthworm active peptide antiseptic feed raw material, with wormcast weight is 100% calculating, 4% wheat bran or the rice chaff that add wormcast weight, add ferment bacterium 0.5%, water 50% continues fermentation, and temperature is controlled between 40-65 ℃
6. add the earthworm body by a certain percentage, add earthworm liquid in the present embodiment, earthworm liquid is that earthworm is placed in the water, add brown sugar, make the earthworm in the water be melted into liquid, add biological bacteria again, the weight ratio of earthworm and water is 1: 2, the weight ratio of earthworm and brown sugar is 1: 0.3, the weight ratio of earthworm and biological bacteria is 1: 0.2, and in the present embodiment, biological bacteria is a ferment bacterium, the addition of earthworm liquid in producing earthworm active peptide antiseptic feed raw material raw material is the 50-60% of wormcast, percentage by weight;
7. earthworm peptide powder is added and produce in the earthworm active peptide antiseptic feed raw material raw material, stir, the addition of earthworm peptide powder is 4% of a wormcast, percentage by weight;
8. dry, control moisture≤13% is packaged into earthworm active peptide antiseptic feed raw produce.
Embodiment three
The concrete technological process of present embodiment production earthworm active peptide antiseptic feed raw material is as follows:
1. the extraction of coelomic fluid of earthworm, earthworm is placed on the bottom to be had in the chest of filter cloth, is provided with container below the chest, gently brushes the earthworm epidermis with salt solution, the weight ratio of salt and water is 1: 5 in the salt solution, earthworm is subjected to salt solution to be stimulated, and can discharge coelomic fluid, sees through filter cloth, drip in the container and collect, earthworm just is upset and can come to harm, and can continue to raise, and coelomic fluid of earthworm is extracted in recycling;
2. the making of earthworm peptide powder, the coelomic fluid of earthworm that extracts is mixed stirring with wormcast after the sterilization, and the weight ratio between the wormcast after coelomic fluid of earthworm and the sterilization is 1: 2, dries standby, because the coelomic fluid of earthworm contains a large amount of antibacterial peptides, this mixture is called earthworm peptide powder;
3. wormcast fermentation, temperature is controlled at below 65 ℃, fermentation time 48 hours, and the weight of wormcast is calculated as 100%;
4. will produce earthworm active peptide antiseptic feed raw material raw material wormcast high temperature sterilization, temperature is controlled at 100 ℃, kills the harmful bacterium in the wormcast, increases beneficial bacterium;
5. add the earthworm body by a certain percentage, add earthworm powder in the present embodiment, the addition of earthworm powder in producing earthworm active peptide antiseptic feed raw material raw material is 5% of wormcast, percentage by weight;
6. earthworm peptide powder is added and produce in the earthworm active peptide antiseptic feed raw material raw material, stir, the addition of earthworm peptide powder is 7% of a wormcast, percentage by weight;
7. dry, control moisture≤13% is packaged into earthworm active peptide antiseptic feed raw produce.
Embodiment four
The concrete technological process of present embodiment production earthworm active peptide antiseptic feed raw material is as follows:
1. the extraction of coelomic fluid of earthworm, earthworm is placed on the bottom to be had in the chest of filter cloth, is provided with container below the chest, gently brushes the earthworm epidermis with salt solution, the weight ratio of salt and water is 1: 6 in the salt solution, earthworm is subjected to salt solution to be stimulated, and can discharge coelomic fluid, sees through filter cloth, drip in the container and collect, earthworm just is upset and can come to harm, and can continue to raise, and coelomic fluid of earthworm is extracted in recycling;
2. the making of earthworm peptide powder, the coelomic fluid of earthworm that extracts is mixed stirring with wormcast after the sterilization, and the weight ratio between the wormcast after coelomic fluid of earthworm and the sterilization is 1: 3, dries standby, because the coelomic fluid of earthworm contains a large amount of antibacterial peptides, this mixture is called earthworm peptide powder;
3. wormcast fermentation, temperature is controlled at below 65 ℃, fermentation time 48 hours, and the weight of wormcast is calculated as 100%;
4. will produce earthworm active peptide antiseptic feed raw material raw material wormcast high temperature sterilization, temperature is controlled at 85 ℃, kills the harmful bacterium in the wormcast, increases beneficial bacterium;
5. wheat bran or rice chaff and ferment bacterium being added water stirs, add in the raw material of producing earthworm active peptide antiseptic feed raw material, with wormcast weight is 100% calculating, 4% wheat bran or the rice chaff that add wormcast weight, add ferment bacterium 0.5%, water 50% continues fermentation, and temperature is controlled between 40-65 ℃
6. add the earthworm body by a certain percentage, add earthworm powder in the present embodiment, the addition of earthworm powder in producing earthworm active peptide antiseptic feed raw material raw material is 4% of wormcast, percentage by weight;
7. earthworm peptide powder is added to produce in the earthworm active peptide antiseptic feed raw material raw material and stir, the addition of earthworm peptide powder is 5% of a wormcast, percentage by weight;
8. dry, control moisture≤13% is packaged into earthworm active peptide antiseptic feed raw produce.
Claims (2)
1, a kind of production method of earthworm active peptide antiseptic feed raw material, has following technological process: the wormcast fermentation, be packaged into earthworm active peptide antiseptic feed raw produce after the drying, it is characterized in that adding earthworm peptide powder in wormcast, the concrete making and the adding technology flow process of earthworm peptide powder are as follows:
1. the extraction of coelomic fluid of earthworm, earthworm is placed on the bottom to be had in the chest of filter cloth, is provided with container below the chest, gently brushes the earthworm epidermis with salt solution, the weight ratio of salt and water is 1 in the salt solution: 2-6, earthworm is subjected to salt solution to be stimulated, and can discharge coelomic fluid, sees through filter cloth, drip in the container and collect, earthworm just is upset and can come to harm, and can continue to raise, and coelomic fluid of earthworm is extracted in recycling;
2. the making of earthworm peptide powder, wormcast or wheat bran after the coelomic fluid of earthworm that extracts and the sterilization are mixed stirring, and wormcast after coelomic fluid of earthworm and the sterilization or the weight ratio between the wheat bran are 1: 2-3, dry standby, because the coelomic fluid of earthworm contains a large amount of antibacterial peptides, this mixture is called earthworm peptide powder;
3. then earthworm peptide powder is added in the wormcast after fermenting, make earthworm active peptide antiseptic feed raw produce.
2, a kind of earthworm active peptide antiseptic feed raw material is characterized in that this earthworm active peptide antiseptic feed raw material is that the method that limits with claim 1 is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNB2003101155834A CN100352362C (en) | 2003-12-03 | 2003-12-03 | Earthworm active peptide antibacerial feed material and its production method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2003101155834A CN100352362C (en) | 2003-12-03 | 2003-12-03 | Earthworm active peptide antibacerial feed material and its production method |
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| Publication Number | Publication Date |
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| CN1623412A CN1623412A (en) | 2005-06-08 |
| CN100352362C true CN100352362C (en) | 2007-12-05 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406052B (en) * | 2011-10-19 | 2013-08-14 | 冯耀民 | Anti-bacterial peptide hydrolysate of earthworm and feed comprising the same |
| CN102558294B (en) * | 2012-01-21 | 2014-04-23 | 河北科技大学 | Method for rapidly extracting forage antibacterial peptides from fermentation liquid |
| CN103740657A (en) * | 2012-10-17 | 2014-04-23 | 沈阳尊龙生物技术有限公司 | Earthworm biological composite enzyme |
| CN105341553A (en) * | 2015-10-30 | 2016-02-24 | 李晓明 | Production method for producing free amino acid antibacterial peptide nutrient additive for silkworms by self-enzyme method |
| CN109287887A (en) * | 2018-09-26 | 2019-02-01 | 何秋宏 | A kind of feed of grass carp and preparation method thereof |
| CN112889756B (en) * | 2019-12-03 | 2023-03-24 | 泰州市春光生态农业发展有限公司 | Method for extracting live earthworm mucus |
-
2003
- 2003-12-03 CN CNB2003101155834A patent/CN100352362C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 复合蚯蚓液营养成分的分析 宋春阳等.饲料研究，第3期 1997 * |
| 蚯蚓(Earthworm)营养价值及其在水产养殖中的应用效果 杨仕懋.现代渔业信息，第8卷第4期 1993 * |
| 蚯蚓两种抗菌肽的分离纯化及部分性质 张希春等.生物化学与生物物理进展，第29卷第6期 2002 * |
| 蚯蚓体表液收取方法的初步研究 陈秉聪等.农业工程学报，第6卷第2期 1990 * |
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Application publication date: 20050608 Assignee: Zhangzhou Dabeinong Agriculture & Pasture Technology Co., Ltd. Assignor: Sun Zhenjun|Liu Fuli Contract record no.: 2010110000031 Denomination of invention: Earthworm active peptide antibacerial feed material and its production method Granted publication date: 20071205 License type: Exclusive License Record date: 20100315 |
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Granted publication date: 20071205 Termination date: 20121203 |