KR100363197B1 - the extracting method of earthworm's body fluids using water contained activated oxygen - Google Patents

Abstract

The present invention relates to a method for extracting earthworm body fluids, and an active oxygen source capable of effectively extracting earthworm body fluids that can be used for various functional cosmetics or liver cell regenerative medicines using water containing active oxygen without any chemicals. It is an object of the present invention to provide a method for extracting the earthworm body fluid by water containing, in order to achieve the above object the present invention 100 to 500 parts by weight of water containing active oxygen with respect to 100 parts by weight of live earthworm After addition, the mixture was allowed to stand at 15 ° C. to 34 ° C. for at least 5 minutes and then filtered by a conventional method. The filtered liquid was left at 0 ° C. to 4 ° C. for 12 hours to 24 hours, and then filtered through a membrane filter. Supported by water containing active oxygen by centrifuging the liquid in a conventional manner and taking only the inferior liquid By providing a method of extracting the fluid can be achieved.

Description

The extracting method of earthworm's body fluids using water contained activated oxygen}

The present invention relates to a method for extracting earthworm body fluid, and more particularly, containing active oxygen, which makes it possible to effectively extract earthworm body fluid that can be used for various functional cosmetics or liver cell regenerative medicines using water containing active oxygen. The present invention relates to a method of extracting earthworm body fluid by water.

An earthworm is a herbaceous animal that has a magnetic organ and a male organ in the form of an invertebrate annulus called a tortoise, a terrestrial or a white rhinoceros, and lives by ingesting bushy organic matter in the fertile wetland soils of various regions.

In particular, according to medical texts such as Dongbogam and herbaceous wood, earthworms contain medicinal ingredients, which are effective for hemolysis and fever, as well as for insecticides, fever, luminescence, jaundice, seasonal infectious diseases, and sore throat. Doing.

In addition to the active medicinal ingredients as described above, the effluent of the earthworm contains an enzyme component such as protease, and researches are actively under way to extract and use them as raw materials for cosmetics.

In particular, protease is a generic term for proteolytic enzymes having proteolytic activity such as papain and trypsin. When these substances are applied to the epidermis, the protease decomposes proteins such as keratin, which is exfoliation or keratin of the epidermis, and is digested into a kind of inducible protein By performing peptone, a nutritious ingredient that can be easily absorbed, it not only helps smooth the epidermal budding and maintains the normalization of skin function but also shows excellent moisture control as a moisturizing additive. It is a substance becoming.

In this regard, as an example technique for experimentally proving the skin activity of the protease-inducing substance described above, Korean Patent Publication No. 88-2222 discloses a protease peptone which is a decomposition-activated substance in cosmetics containing a moisturizing agent as one of the active ingredients. Is added to 0.01 to 10% by weight to preserve the moisture in the air when the humidity is low evaporation, when high humidity does not absorb more than a certain amount of skin care cosmetics that can always maintain the moisture content on the skin in the desired range It was provided.

As described above, a technique for effectively extracting a protease component showing excellent function as a cosmetic from earthworm and applying it to the manufacture of cosmetics has been steadily introduced, and is represented in Japanese Laid-Open Patent Publication No. 63-238009. After heat treatment of the earthworm by a known technique, it is left to stand in an ethanol aqueous solution for about 2 weeks in a fine state, and water is added to the aqueous layer obtained by centrifugation by adding water to the aqueous layer. Introduction of cosmetics containing earthworm extracts that show the effects of adsorption moisturizing, glossiness, anti-skin tanning and erythema prevention by mixing the extract containing protease with conventional cosmetics. Doing.

However, the above-described prior art includes a chemical treatment process such as chloroform as a water-insoluble organic solvent, which is toxic in the extraction process of the protease, and thus, in some cases, there is a problem that additionally requires a toxic neutralization process. By cutting the earthworms, a process such as a long time filtration treatment for removing impurities such as epidermal pulverization of the earthworms, which is inevitably generated, is also required, which acts as an increase factor of the production cost.

Accordingly, the present invention is to solve the above-mentioned problems, so that the earthworm body fluid that can be used for various functional cosmetics and hepatocyte regenerative medicines can be effectively extracted using water containing active oxygen without adding any chemicals. An object of the present invention is to provide a method for extracting earthworm body fluid by water containing an active oxygen.

In order to achieve the above object, the present invention adds 100 parts by weight to 500 parts by weight of water containing active oxygen with respect to 100 parts by weight of live earthworms, and the mixture is allowed to stand at 15 ° C. to 34 ° C. for at least 5 minutes and then filtered by a conventional method. The filtered liquid is left at 0 ° C. to 4 ° C. for 12 hours to 24 hours, and then filtered again using a membrane filter, and the filtered liquid contains active oxygen by centrifugation in a conventional manner to take only a lower liquid. This can be achieved by providing a method for extracting earthworm body fluid by water.

In the present invention, first, 100 parts by weight to 500 parts by weight of water containing active oxygen is added to 100 parts by weight of live earthworms, and the mixture is left to stand at 15 ° C. to 34 ° C. for at least 5 minutes and then filtered by a conventional method.

At this time, the earthworm may use any kind of earthworm, but in particular, the red earthworm of February to 4 months, which is farmed by the method proposed in Korean Patent Application No. 2000-0039440, filed by the present inventor (Lumbricus Rubellus Is preferably used.

In the present invention, the red earthworm cultured as described above is put into water containing active oxygen to extract the earthworm body fluid, and the water containing the active oxygen can be prepared by various methods, and the general method is a strong magnetic field or far infrared rays. It generates the longest electromagnetic wave.

In this way, the water molecules that make up the large aggregates are stimulated to vibrate for a long time to change into monomolecules or small aggregates of water molecules.At the same time, some of the water is decomposed into hydrogen ions and hydroxide ions. Decomposition occurs to generate hydrogen and oxygen, and the produced oxygen has very active properties.

In particular, the active oxygen-containing water used in the present invention was water used for water treatment with a ceramic produced in the "ceramic body having a water-repellent function and its manufacturing method" of Korean Patent No. 226423.

When the earthworm is placed in the water containing the active oxygen, the earthworm absorbs the water, and at this time, the earthworm cells are oxidized by some active oxygen present in the activated activated water molecules, and the earthworm detoxifies oxidation. To fight against antioxidant enzymes.

The same phenomenon occurs in the body surface of the earthworm, but one difference is that when active oxygen oxidizes the body surface cells, the earthworm receives this oxidation as a physical and chemical stimulus and releases a large amount of body fluid, a self-defense substance.

In this case, in order to maximize the amount of body fluid released, it is preferable to add 100 parts by weight to 500 parts by weight with respect to 100 parts by weight of active earthworms, and the amount of body fluid extracted when the amount is less than 100 parts by weight. This problem is not enough, and when added in excess of 500 parts by weight of the earthworm's dying speed is a problem that the body fluid does not come out continuously.

After adding active oxygen-containing water, the mixture is allowed to stand at 15 ° C. to 34 ° C. for at least 5 minutes. The temperature is considered to be such that the earthworm does not heat up by the temperature, and the settling time is the amount of body fluid released. In consideration of this, it is preferable to settle for 5 minutes or more.

In the process of standing in the active water, the earthworm ends its life while releasing a large amount of body fluid, which is filtered in a conventional manner to remove impurities including the earthworm body contents, including the earthworm body contents.

As described above, the living earthworms are placed in water containing active oxygen to express a large amount of body fluid, and the filtered liquid is left for 12 hours to 24 hours at 0 ° C. to 4 ° C., and then filtered through a membrane filter.

The body fluid filtered by the membrane filter contains a red pigment (blood cell), a protein, an enzyme, and the like. The filtrate may be denatured or denatured when the filtrate is stored at a high temperature. It is preferable to leave it to stand at the temperature of ° C.

In particular, the blood cells contained in the filtered body fluid acts with proteolytic enzymes or fibrinolytic enzymes to decrease the titer of enzymes. To prevent this, the blood cells are allowed to stand for 12 to 24 hours to coagulate and settle the red pigment (blood cells) in advance. Is preferably removed.

The filtrate left as described above is filtered again and centrifuged in a conventional manner to take only the inferior liquor so that the earthworm body fluid can be extracted using water containing the active oxygen according to the present invention.

In this case, the extracted body fluid can be obtained by lyophilization in a conventional manner, and the powdery earthworm extract can be obtained, and even using the earthworm body fluid or the powdery extract can be used for various purposes including cosmetic additives.

Hereinafter, the present invention will be described in detail with reference to the following examples, but the following examples are presented to aid the understanding of the present invention, but the present invention is not limited to the following description.

<Example 1>

Wash the epidermis and add 2 kg of active water to 1 kg of the earthworm to remove foreign substances, and leave it at 30 ° C. for 5 minutes, and then filter. The filtered liquid is left at 0 ° C. for 18 hours, and then filtered through a membrane filter. The liquid thus obtained was centrifuged in a conventional manner to obtain only the supernatant liquid to obtain 0.18 kg of liquid earthworm body fluid extract.

<Example 2>

Wash the epidermis and add 1.5kg of active water to 1kg of debris to remove foreign substances, and then, stand still at 30 ° C for 5 minutes, and then filter, and the filtered liquid is left at 0 ° C for 18 hours, and then filtered with a membrane filter. The filtered liquid was centrifuged by a conventional method, and only the lower liquor was taken and lyophilized to obtain 0.17 kg of powdery earthworm body fluid extract.

Earthworm body fluid extract obtained in the above examples can be used for various purposes, including cosmetics.

As described above, the present invention provides the extraction of earthworm body fluid by active oxygen-containing water, which makes it possible to effectively extract earthworm body fluid that can be used for various functional cosmetics or hepatocyte regenerative medicines using active water containing active oxygen. It is a useful invention to provide a method.

Claims (2)

100 parts by weight to 500 parts by weight of water containing active oxygen is added to 100 parts by weight of earthworms, and the mixture is left to stand at 15 ° C. to 34 ° C. for at least 5 minutes and then filtered by a conventional method. After 12 hours to 24 hours at ℃ ℃ filtered by a membrane filter, the filtered liquid is centrifuged by a conventional method to extract the earthworm body fluid by active oxygen-containing water, characterized in that to take only the inferior liquid . The method of extracting earthworm body fluid by active oxygen-containing water, according to claim 1, wherein the lower liquid is lyophilized to obtain a powdery extract.