CN101779628A - Method for preparing gallic acid aquiculture sanitizer and product thereof - Google Patents
Method for preparing gallic acid aquiculture sanitizer and product thereof Download PDFInfo
- Publication number
- CN101779628A CN101779628A CN200910226625A CN200910226625A CN101779628A CN 101779628 A CN101779628 A CN 101779628A CN 200910226625 A CN200910226625 A CN 200910226625A CN 200910226625 A CN200910226625 A CN 200910226625A CN 101779628 A CN101779628 A CN 101779628A
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- CN
- China
- Prior art keywords
- gallic acid
- products
- aquiculture
- filtrating
- sanitizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 229940074391 gallic acid Drugs 0.000 title claims abstract description 26
- 235000004515 gallic acid Nutrition 0.000 title claims abstract description 26
- 238000009360 aquaculture Methods 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title claims abstract description 7
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000012530 fluid Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 238000000605 extraction Methods 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims abstract description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 238000001728 nano-filtration Methods 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000000284 extract Substances 0.000 claims description 8
- 238000000874 microwave-assisted extraction Methods 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 5
- 238000000502 dialysis Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 239000003995 emulsifying agent Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 2
- 238000001471 micro-filtration Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 14
- 201000010099 disease Diseases 0.000 abstract description 10
- 241000251468 Actinopterygii Species 0.000 abstract description 8
- 208000017520 skin disease Diseases 0.000 abstract description 8
- 206010063409 Acarodermatitis Diseases 0.000 abstract description 4
- 241000238557 Decapoda Species 0.000 abstract description 4
- 241000447727 Scabies Species 0.000 abstract description 4
- 208000005687 scabies Diseases 0.000 abstract description 4
- 229920002492 poly(sulfone) Polymers 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000000843 powder Substances 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract 2
- 241000270666 Testudines Species 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 description 14
- 239000003814 drug Substances 0.000 description 11
- 239000000645 desinfectant Substances 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 4
- 241001482311 Trionychidae Species 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229940084434 fungoid Drugs 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 210000004379 membrane Anatomy 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000007598 dipping method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002790 anti-mutagenic effect Effects 0.000 description 1
- 230000001857 anti-mycotic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000002543 antimycotic Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 230000003559 chemosterilizing effect Effects 0.000 description 1
- 230000002279 cholagogic effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention provides a method for preparing gallic acid aquiculture sanitizers and products thereof, which comprises the following steps: filtrating gallnut by microwave centrifugation; micro-filtrating; fine-filtrating; ultra-filtrating the fine-filtrated liquid with polysulphone membrane of 50,000 molecular weight, and recycling the trapped fluid by ultra-filtration for extraction; and nano-filtrating the ultra-filtrated dialysate, and recycling the nano-filtrated dialysate for extraction, thus obtaining the products. The products can be made into aqua and powder. The products of the invention have good control effect on various diseases, such as gill-rot disease, white skin disease, red skin disease, scabies disease, fungous saprolegniasis, branchiomycosis and the like, from aquatic animals, such as fish, shrimp, crab, turtle, and frog, etc. Besides, the products do not cause water environmental pollution due to little residue in the aquatic products, so that quality of the products is stable and controllable.
Description
Technical field
The present invention relates to a kind of preparation of high-efficiency low-toxicity aquatic products disinfectant, particularly prepare method of aquatic products disinfectant and products thereof with the natural drug gallic acid.
Background technology
China is world aquaculture big country, and output accounts for 80% of world's cultured output.Along with the continuous expansion of aquaculture scale and improving constantly of intensification degree, add going from bad to worse of breeding environment, the aquiculture disease incidence of disease is more and more higher, cause of disease is based on bacteriosis, the whole nation monitored 144 kinds of diseases in 2003, wherein bacteriosis is totally 60 kinds, accounts for 41.7%, secondly is 39 kinds of parasitic diseases.Culture the direct economic loss that disease causes then and reach 105.84 hundred million yuan.In addition, because a large amount of chemosterilant and chemicalses of using in the aquaculture process, many catabolites have the mutagenesis carcinogenic effect, can produce serious harm to aquatic animal and health, and safety in production brings many hidden danger to aquatic products.
Gallic acid has another name called gallate, gallic acid, its chemistry by name 3,4, the 5-trihydroxybenzoic acid, molecular weight 188.1 is a kind of important fine chemistry industry basic products, medicinal compound with natural activity composition has many-sided pharmacological actions such as stronger antibiotic, anti-inflammatory, antiviral, antimycotic, anti-oxidant, antitumor, antiallergy, antimutagenic, hemostasis, convergence, cholagogic clinically at medicine.Gall nut is the distinctive conventional Chinese medicine material of China, be rich in gallic acid, content is up to 65%, antibiotic, sterilization, detoxication with resisting gram-positive and negative bacterium, to fish skin, mucous membrane, ulcer to good astriction is arranged, the his-and-hers watches dermophyte has inhibitory action, can quicken blood coagulation and have anastalsis.At present the existing gall nut marketable fish medicine of China " control mould clean ", folk prescription and compound preparation such as " Yu Anqing ", the diseases such as fin rot, white skin disease, red skin disease, scabies disease and fungoid saprolegniasis, branchiomycosis that are used to prevent and treat aquatic livestocks such as fish, shrimp, crab, soft-shelled turtle, the frog.These Chinese herbal medicine aquatic products fish medicines all are with raw medicinal herbs direct drug injections such as gall nut, and the processing formulation falls behind, and drug effect is slow, and bioavilability is low, the difficult control of medicine solubility and product quality, control efficiency instability.Be badly in need of in the production that exploitation is safe, efficient, quick-acting, low-residual, quality controllable green aquatic products fish medicine pharmaceutical technology and product.
Summary of the invention
The object of the present invention is to provide a kind of natural drug gallic acid to prepare method of aquatic products disinfectant and products thereof, prepared bactericide has good control efficiency to diseases such as fin rot, white skin disease, red skin disease, scabies disease and the fungoid saprolegniasis of aquatic livestocks such as fish, shrimp, crab, soft-shelled turtle, the frog, branchiomycosises, and it is pollution-free to water environment, and technology is simple relatively, and cost is relatively low.
Embodiment of the present invention are: a kind of preparation method of gallic acid aquiculture sanitizer may further comprise the steps:
(1) with gall nut through pulverizing, cross 40 mesh sieves;
(2) drop into microwave extraction tank, the pure water that adding gall nut raw material weight is 8-12 times and the concentration of gall nut raw material weight 1% are 30～35% liquid caustic soda, stir and extract, microwave frequency 2450MHz, microwave power and material load 20L/KW, extraction time 30-60min;
(3) extract back centrifugal filtration, micro-filtration, smart filter;
(4) get smart filtrate earlier with 50,000 molecular weight PS membrane ultrafiltration, the reuse of ultrafiltration trapped fluid is extracted, the nanofiltration again of ultrafiltration dialysis liquid, and the reuse of nanofiltration dislysate is extracted.
With tinning behind step (4) direct can of gained nanofiltration trapped fluid or the adding emulsifier, promptly get gallic acid disinfection aqua.
With step (4) gained nanofiltration trapped fluid vacuum concentration, atomized drying, pack promptly gets gallic acid disinfection pulvis.
Using method of the present invention is:
Gallic acid aqua: as every cubic metre of water body 2ml of disinfectant consumption, as every cubic metre of water body 5ml of bactericide dipping bath consumption.
Gallic acid pulvis: as every cubic metre of water body consumption of disinfectant consumption 0.5g, as every cubic metre of water body 1.5g of bactericide dipping bath consumption.
The present invention is a raw material with natural products gall nut traditional Chinese medicine, take microwave rapid extraction natural drug gallic acid active ingredient, in addition the preparation of science compatibility is used for aqua, the wetting powder of aquaculture disinfection, diseases such as fin rot, white skin disease, red skin disease, scabies disease and the fungoid saprolegniasis of aquatic livestocks such as fish, shrimp, crab, soft-shelled turtle, the frog, branchiomycosis had good control efficiency, and it is pollution-free to water environment, the basic noresidue of aquatic products, constant product quality is controlled.
Embodiment
The invention will be further described below in conjunction with example.
Embodiment 1: the preparation of gallic acid aqua
Gall nut 200g pulverizes, crosses 40 mesh sieves, drops into power 1200W, and volume 5L microwave extraction tank adds 2000ml pure water, 22ml liquid caustic soda, stirs and extracts, microwave frequency 2450MHz, Microwave Extraction time 30min.Extract back coarse filtration, smart filter, with 50,000 molecular weight PS membrane ultrafiltration, ultrafiltration dialysis liquid is used 500 molecular weight polysulfones film nanofiltrations again; The nanofiltration trapped fluid adds the emulsifier bottling, gets gallic acid aqua 752ml, gallic acid content 12.5%.
Embodiment 2: the preparation of gallic acid pulvis
Gall nut 200g pulverizes, crosses 40 mesh sieves, drops into power 1200W, and volume 5L microwave extraction tank adds 2000ml pure water, 22ml liquid caustic soda, stirs and extracts, microwave frequency 2450MHz, Microwave Extraction time 60min.Extract back coarse filtration, smart filter, with 50,000 molecular weight PS membrane ultrafiltration, ultrafiltration dialysis liquid is used 500 molecular weight polysulfones film nanofiltrations again; The nanofiltration trapped fluid concentrates, and atomized drying gets gallic acid pulvis 95.6g, gallic acid content 83.6%.
Claims (4)
1. the preparation method of a gallic acid aquiculture sanitizer is characterized in that, may further comprise the steps:
(1) with gall nut through pulverizing, cross 40 mesh sieves;
(2) drop into microwave extraction tank, the pure water that adding gall nut raw material weight is 8-12 times and the concentration of gall nut raw material weight 1% are 30～35% liquid caustic soda, stir and extract, microwave frequency 2450MHz, microwave power and material load 20L/KW, extraction time 30-60min;
(3) extract back centrifugal filtration, micro-filtration, smart filter;
(4) get smart filtrate earlier with 50,000 molecular weight PS membrane ultrafiltration, the reuse of ultrafiltration trapped fluid is extracted, the nanofiltration again of ultrafiltration dialysis liquid, and the reuse of nanofiltration dislysate is extracted.
2. the preparation method of gallic acid aquiculture sanitizer according to claim 1 is characterized in that, with tinning behind step (4) direct can of gained nanofiltration trapped fluid or the adding emulsifier, promptly gets gallic acid disinfection aqua.
3. the preparation method of gallic acid aquiculture sanitizer according to claim 1 is characterized in that, with step (4) gained nanofiltration trapped fluid vacuum concentration, and atomized drying, pack promptly gets gallic acid disinfection pulvis.
4. gallic acid aquiculture sanitizer as claim 1 or the preparation of 2 or 3 described methods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910226625 CN101779628B (en) | 2009-12-10 | 2009-12-10 | Method for preparing gallic acid aquiculture sanitizer and product thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910226625 CN101779628B (en) | 2009-12-10 | 2009-12-10 | Method for preparing gallic acid aquiculture sanitizer and product thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101779628A true CN101779628A (en) | 2010-07-21 |
| CN101779628B CN101779628B (en) | 2013-04-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910226625 Active CN101779628B (en) | 2009-12-10 | 2009-12-10 | Method for preparing gallic acid aquiculture sanitizer and product thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101779628B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948159A (en) * | 2010-07-26 | 2011-01-19 | 中国水产科学研究院淡水渔业研究中心 | Water-sprinkling anti-disease antidote for freshwater shrimp/crab farming |
| CN104257812A (en) * | 2014-09-29 | 2015-01-07 | 浙江海洋学院 | Fish medicine for treating branchiomycosis |
| CN107006538A (en) * | 2017-05-27 | 2017-08-04 | 明光市裕阳农业有限公司 | A kind of plant environment-friendly type bactericide used for aquiculture and preparation method thereof |
| CN107348236A (en) * | 2017-07-20 | 2017-11-17 | 通化师范学院 | Chinese herbal medicine feed additive for preventing and treating freshwater salmon red skin disease and its application |
| CN110664937A (en) * | 2019-11-25 | 2020-01-10 | 湖南利农五倍子产业发展有限公司 | Compound aquatic bactericide containing gallic acid and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1037601C (en) * | 1993-11-17 | 1998-03-04 | 中国林业科学研究院林产化学工业研究所 | Preparation of gallic acid by basic hydrolyzation |
-
2009
- 2009-12-10 CN CN 200910226625 patent/CN101779628B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948159A (en) * | 2010-07-26 | 2011-01-19 | 中国水产科学研究院淡水渔业研究中心 | Water-sprinkling anti-disease antidote for freshwater shrimp/crab farming |
| CN104257812A (en) * | 2014-09-29 | 2015-01-07 | 浙江海洋学院 | Fish medicine for treating branchiomycosis |
| CN107006538A (en) * | 2017-05-27 | 2017-08-04 | 明光市裕阳农业有限公司 | A kind of plant environment-friendly type bactericide used for aquiculture and preparation method thereof |
| CN107348236A (en) * | 2017-07-20 | 2017-11-17 | 通化师范学院 | Chinese herbal medicine feed additive for preventing and treating freshwater salmon red skin disease and its application |
| CN110664937A (en) * | 2019-11-25 | 2020-01-10 | 湖南利农五倍子产业发展有限公司 | Compound aquatic bactericide containing gallic acid and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101779628B (en) | 2013-04-03 |
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Address after: 427000 Xixi Zhangjiajie economic and Technological Development Zone, Yongding District, Zhangjiajie, Hunan Patentee after: JIURUI BIOLOGY & CHEMISTRY CO.,LTD. Address before: 427000 Xixi Zhangjiajie economic and Technological Development Zone, Yongding District, Zhangjiajie, Hunan Patentee before: ZHANGJIAJIE AOWEI SCIENCE & TECHNOLOGY Co.,Ltd. |
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Denomination of invention: Preparation method and product of Gallic acid aquatic disinfectant Effective date of registration: 20230628 Granted publication date: 20130403 Pledgee: Bank of Communications Co.,Ltd. Zhangjiajie branch Pledgor: JIURUI BIOLOGY & CHEMISTRY CO.,LTD. Registration number: Y2023980046143 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
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Granted publication date: 20130403 Pledgee: Bank of Communications Co.,Ltd. Zhangjiajie branch Pledgor: JIURUI BIOLOGY & CHEMISTRY CO.,LTD. Registration number: Y2023980046143 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation Method and Product of Gallic Acid Aquatic Disinfectant Granted publication date: 20130403 Pledgee: Bank of Communications Co.,Ltd. Zhangjiajie branch Pledgor: JIURUI BIOLOGY & CHEMISTRY CO.,LTD. Registration number: Y2024980025097 |