CN112868656A - Bactericidal compound agent containing alginate oligosaccharides and neooxonol peptides and application thereof - Google Patents
Bactericidal compound agent containing alginate oligosaccharides and neooxonol peptides and application thereof Download PDFInfo
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- CN112868656A CN112868656A CN201911201690.6A CN201911201690A CN112868656A CN 112868656 A CN112868656 A CN 112868656A CN 201911201690 A CN201911201690 A CN 201911201690A CN 112868656 A CN112868656 A CN 112868656A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
Abstract
The invention relates to a sterilization compound agent containing alginate oligosaccharide, wherein the alginate oligosaccharide comprises sodium alginate oligosaccharide and derivatives thereof, and neooxynucleoside antibiotic. The two are mixed in different proportions to form a soluble compound agent, which has synergistic effect on preventing and treating various diseases such as rice sheath blight, wheat scab, cucumber angular leaf spot, tomato gray mold, tomato virus disease, tobacco virus disease and the like, and simultaneously improves the crop yield. The dosage of alginic acid oligosaccharide in the compound agent is 1.0-10%, the dosage of neoobioside peptide is 1.0-10%, and the compound preparation of alginic acid oligosaccharide and derivatives thereof and neoobioside peptide expands the antibacterial and bactericidal range and improves the control effect; the self-defense reaction of the crops is improved, the drought resistance, high temperature resistance and other stress resistances of the crops are improved, the growth of the crops is promoted, and the yield and the quality of the crops are improved; meanwhile, the usage amount of chemical pesticides can be reduced, the environmental pollution is reduced, and the development of environment-friendly novel compound bactericide is facilitated.
Description
Technical Field
The invention relates to a preparation method of a compound agent taking alginate oligosaccharide and bactericide neoobioside as effective components and application of the compound agent in preventing and treating phytopathogen.
Background
Plant diseases are a large factor restricting agricultural sustainable development, in order to effectively control the plant diseases, chemical pesticides are mostly applied to control the plant diseases in production, the chemical pesticides are used in a large amount, plant pathogenic bacteria generate variation and increase of drug resistance, the diseases are serious, the environment is polluted, the food safety problem is serious, and the search for safe, efficient and environment-friendly novel pesticides is important.
The neooxonoside peptide is a novel biological bactericide, is an antibacterial compound with a new structure produced by fermenting genetically improved strain Streptomyces norroseus XiAo-3 (streptomyces noursei XiAo-3), is a uracil nucleoside peptide antibiotic with a new chemical structure, and has a relative molecular mass of 445 and a molecular formula of C16H23N5O10(1-uracil-4 sarcosyl-serylamino-1, 4-dideoxy-. beta. -D-glucopyranosuronic acid). Neoeoside peptide is a broad-spectrum bactericide which can inhibit the synthesis of fungal cell walls by inhibiting pathogenic bacteria chitin protease, inhibit the growth of pathogenic bacteria, and simultaneously has certain induced disease resistance to control virus diseases. The main problems existing at present are that the inhibition effects of the neooxonoside peptide on different pathogenic bacteria are different, so that the variety of the diseases is less, the control effect of the neooxonoside peptide on the diseases of crops is not ideal, the control effect of the neooxonoside peptide on the gibberellic disease of wheat is about 50 percent, the control effect on rice sheath blight is 20 to 40 percent, the control effect on rice false smut is about 50 percent, the control effect on sclerotinia rot of colza is 68 percent, the control effect on phytophthora capsici is 59 percent, the control effect on phytophthora blight of eggplant is 39 percent and the control effect on botrytis cinerea is 27 percent. The effect of preventing and controlling other diseases is not ideal, and the effect of preventing and controlling other diseases is not stable, and the effect of preventing and controlling common diseases of other economic crops is not good. Thus, the pesticide needs to be sprayed for multiple times and dosage, the cost is increased, the influence on the environment is increased, and the pesticide spraying device has the same effect as the pesticide spraying deviceThe quality of the agricultural products is greatly influenced.
Oligosaccharide substances are applied to the field of plant protection in the last 70 th century, have the functions of inhibiting plant pathogenic bacteria, inducing plant disease resistance, promoting plant growth and the like, are specially named as oligosaccharide biopesticides, and have the advantages of small relative molecular mass, low toxicity, good water solubility, strong absorptivity and the like. The alginic acid oligosaccharide has the activity of inducing plant disease resistance, also has the functions of promoting growth and improving plant drought resistance, is mixed with bactericide or antibiotic, improves the effect of the bactericide, can reduce the dosage of the bactericide or antibiotic, and enlarges the prevention range of some bactericides. The alginic acid oligosaccharide and the neoaugustide are compounded, so that the characteristics of the alginic acid oligosaccharide and the neoaugustide can be exerted, a good effect can be exerted on the prevention and the treatment of plant diseases, the drought resistance of crops is improved, the growth of the crops is promoted, and the yield is increased. The alginic acid oligosaccharide and neoaugustide are compounded as a novel pesticide for preventing and treating plant diseases, and the reports of documents and patents are not found.
Disclosure of Invention
The invention aims to provide a safe and effective compound pesticide which is prepared by mixing alginate oligosaccharide and neoobioside peptide according to a certain proportion to form a soluble aqueous solution and is used for preventing and treating plant diseases, and a using method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
1. the broad-spectrum oligosaccharide compound bactericide has active ingredients of alginic acid oligosaccharide and neoaugustide; wherein the content of alginic acid in the compound agent is 1.0-10%; the content of neooxonol glycoside in the compound agent is 1.0-10%.
2. The broad-spectrum bactericide provided by the invention can be used for grain crops, economic trees, traditional Chinese medicinal materials, flowers or lawns; when the disease occurs, one or more than two of fungal diseases such as banded sclerotial blight, powdery mildew, banded sclerotial blight, gibberellic disease and the like, bacterial diseases such as angular leaf spot, canker and the like and virus diseases such as mosaic disease and the like can be effectively prevented and controlled.
3. The broad-spectrum bactericide provided by the invention has double effects of protection and treatment on crop diseases, can protect healthy plants from being infected by pathogenic bacteria before disease occurrence, can control disease spread after disease occurrence, is not easy to generate drug resistance by pathogenic bacteria, can effectively improve plant immunity while preventing and treating diseases, and induces plants to generate disease resistance and cold resistance; and has the function of promoting growth. The plant yield increasing rate reaches 10-30%.
5. The application method of the broad-spectrum bactericide disclosed by the invention is one or more than two treatment modes of spraying, seed soaking, root dipping and root irrigation, and is used for treating one or more than two of plant seeds, seedlings or adult plants; diluted 1000 times before administration.
The invention relates to a sterilization compound agent containing alginate oligosaccharide, wherein the alginate oligosaccharide comprises sodium alginate oligosaccharide and derivatives thereof, and neooxynucleoside antibiotic. The two are mixed in different proportions to form a soluble compound agent, which has synergistic effect on preventing and treating various diseases such as rice sheath blight, wheat scab, cucumber angular leaf spot, tomato gray mold, tomato virus disease, tobacco virus disease and the like, and simultaneously improves the crop yield. The dosage of alginic acid oligosaccharide in the compound agent is 2.0-10%, the dosage of neoobioside peptide is 1.0-10%, and the compound preparation of alginic acid oligosaccharide and derivatives thereof and neoobioside peptide expands the antibacterial and bactericidal range and improves the control effect; the self defense reaction of the crops is improved, the drought resistance of the crops is improved, the growth of the crops is promoted, and the yield and the quality of the crops are improved; meanwhile, the usage amount of chemical pesticides can be reduced, the environmental pollution is reduced, and the development of environment-friendly novel compound bactericide is facilitated.
The invention has the following advantages:
1 has the functions of preventing diseases and promoting growth, and is green, environment-friendly and environment-friendly.
2 the compound preparation is aqueous, does not need to add a large amount of auxiliary agents, is simple and convenient to apply, and is suitable for large-scale popularization and application.
3 the invention not only acts on pathogenic microorganism directly, but also can adjust plant immunity, induce plant disease resistance, has more obvious and lasting drug effect, reduces the usage amount of pesticide, and does not generate drug resistance.
4 the effect of the compound agent is equivalent to that of a pure chemical preparation, the compound agent can partially replace chemical pesticides, pesticide residues are reduced, and the problem of food safety is solved.
Detailed Description
The invention will be further illustrated by the following examples
Example 1 field Effect of sodium alginate oligosaccharide and NeoOstrin Compound preparation on wheat sheath blight disease
The test is carried out in the wheat field of Changwu county in Shaanxi province, and the test is provided with 6 treatments (mass concentration), (1) 4.0% of neoornidazole and 2.5% of sodium alginate oligosaccharide are diluted 1200 times; (2) diluting by 1000 times with 4.0% neooxylside and 2.5% sodium alginate oligosaccharide; (3) diluting by 800 times with 4.0% neooxolanyl peptide and 2.5% sodium alginate oligosaccharide; (4) 4.0% neooxroside peptide diluted 1000 times; (5) 2.5% sodium alginate oligosaccharide is diluted 1000 times; (6) and (5) clear water control. And (3) repeating the treatment every 3 times, dressing seeds during sowing, spraying for the first time for applying the pesticide at the early stage of the wheat sharp eyespot, spraying for 1 time every 7-10 days, continuously spraying for 3 times, investigating the disease condition of the wheat sharp eyespot on the 10 th day after the third pesticide application, and calculating the control effect.
TABLE 1 prevention and treatment of Rhizoctonia cerealis by sodium alginate oligosaccharide and NeoOstrin compound preparation
The prevention effect is {1- (disease index before treatment drug X disease index after treatment drug)/(disease index before treatment drug X disease index after treatment drug) } X100/100
The field efficacy test result of the sodium alginate oligosaccharide and neoaugustine compound preparation on wheat sharp eyespot shows that the sodium alginate oligosaccharide and neoaugustine compound preparation has a synergistic effect, the treatment control effect of the alginic acid oligosaccharide compound preparation is higher than that of the single use of the neoaugustine and the alginic acid oligosaccharide, and the maximum treatment control effect of 100 g/mu is 79.04%.
Example 2 sodium alginate oligosaccharide and Neotaxin Compound preparation for inhibiting Sclerotinia sclerotiorum, Colletotrichum malabaricum, Gibberella tritici and tobacco Heilobium tabacum
The inhibition effect of the sodium alginate oligosaccharide and neoaugustide compound preparation on the following nine germs is determined according to the method of the example 1, and the toxicology is calculated.
TABLE 2 measurement of the inhibition of the oligosaccharide and neooxonol peptide against nine pathogenic bacteria
Example 3 field test result of sodium alginate oligosaccharide and NeoOstrin Compound preparation for Phytophthora capsici disease
The test is carried out in the Chili pepper field in Qishan county of Shanxi province, and the test is provided with 6 treatments (mass concentration), (1)4.0 percent of neoornithogatide and 1 percent of sodium alginate oligosaccharide are diluted by 1000 times; (2) diluting by 1000 times with 1% neoaugustide and 4% sodium alginate oligosaccharide; (3)2.5 percent of neoobinoside and 2.5 percent of sodium alginate oligosaccharide are diluted by 1000 times; (4) 4.0% neooxroside peptide diluted 1000 times; (5) 2.5% sodium alginate oligosaccharide is diluted 1000 times; (6) and (5) clear water control. Repeating the treatment for 3 times, spraying the pesticide for the first time at the initial stage of the pepper phytophthora blight disease, spraying the pesticide for 1 time every 7-10 days, continuously spraying the pesticide for 3 times, investigating the disease condition of the pepper phytophthora disease on the 10 th day after the third pesticide application, calculating the control effect, and obtaining a test result of 3.
TABLE 3 prevention of phytophthora blight of pepper by compounding sodium alginate oligosaccharide and neooxonoside peptide
The test result shows that the sodium alginate oligosaccharide and the neooxonol glycoside peptide compound have synergistic effect on the control effect of the pepper phytophthora blight. The compound treatment of 2.5 percent of alginic acid oligosaccharide and 2.5 percent of neoobioside peptide has the highest prevention effect, and the prevention effect is 80.14 percent.
Example 4 prevention and treatment effects of sodium alginate oligosaccharide and NeoOstrin on Sclerotinia sclerotiorum
The test is carried out in Shaanxi Ankang, and 6 treatments (mass concentration) are arranged in total, (1) 4.0% of neoornithogatide and 1% of sodium alginate oligosaccharide are diluted by 1000 times; (2) diluting by 1000 times with 1% neoaugustide and 4% sodium alginate oligosaccharide; (3)2.5 percent of neoobinoside and 2.5 percent of sodium alginate oligosaccharide are diluted by 1000 times; (4) 4.0% neooxroside peptide diluted 1000 times; (5) 2.5% sodium alginate oligosaccharide is diluted 1000 times; (6) and (5) clear water control. Spraying 3 times in the seedling stage (three leaf stage), budding stage and flowering stage of rape after 3 times of repeated treatment, investigating the incidence rate and disease index of sclerotinia rot of rape 10 days after the last spraying, calculating the prevention and treatment effect, and performing field test on sclerotinia rot of rape by using the sodium alginate oligosaccharide and neooxonoside peptide compound preparation, wherein the test result is shown in 4.
TABLE 4 prevention of Sclerotinia sclerotiorum by sodium alginate oligosaccharide and Neooxonol peptide
Test results show that the compound preparation of the sodium alginate oligosaccharide and the neooxolanyl peptide has synergistic effect on the prevention effect of the sclerotinia rot of rape, the prevention effect of the compound treatment of the alginate oligosaccharide and the neooxolanyl peptide is 64.34 to 73.33 percent, which is higher than 54.84 and 29.85 percent of the prevention effect of the single treatment of the neooxolanyl peptide and the alginate oligosaccharide, and the prevention effect of the compound treatment of 2.5 percent of the alginate oligosaccharide and 2.5 percent of the neooxolanyl peptide is the highest, and is 73.33 percent.
Example 5 prevention and treatment of tomato Virus diseases with sodium alginate oligosaccharide and NeoOstrin Compound preparation
The test is carried out in Shandong Shouguang, and the test is totally set with 6 treatments (mass concentration), (1)4.0 percent of neoobioside and 1 percent of sodium alginate oligosaccharide are diluted by 1000 times; (2) diluting by 1000 times with 1% neoaugustide and 4% sodium alginate oligosaccharide; (3)2.5 percent of neoobinoside and 2.5 percent of sodium alginate oligosaccharide are diluted by 1000 times; (4) 4.0% neooxroside peptide diluted 1000 times; (5) 2.5% sodium alginate oligosaccharide is diluted 1000 times; (6) and (5) clear water control. Repeating the treatment for 3 times, starting the first pesticide application at the initial stage of the disease onset, spraying the pesticide for 1 time every 7-10 days, continuously spraying the pesticide for 3 times, investigating the disease onset condition of tomato virus diseases on the 10 th day after the third pesticide application, calculating the control effect, and the test result is shown in table 5.
TABLE 5 prevention of tomato virus disease by sodium alginate oligosaccharide and Neooxonol glycoside peptide compounded preparation
The test result shows that the compound preparation of the alginate oligosaccharide and the neooxolanyl peptide has synergistic effect on the prevention effect of tomato virus diseases, the prevention effect of the compound treatment of the alginate oligosaccharide and the neooxolanyl peptide is 62.10 to 74.58 percent and is higher than the prevention effect of 56.29 and 60.45 percent of the compound treatment of the neooxolanyl peptide and the alginate oligosaccharide, and the prevention effect of the compound treatment of 2.5 percent of the alginate oligosaccharide and 2.5 percent of the neooxolanyl peptide is the highest and is 77.56 percent.
Example 6 prevention and treatment effects of sodium alginate oligosaccharide and NeoOstrin on tobacco mosaic disease
The test is carried out on Yuxi Yunan, and the total number of the test is 6 treatments (mass concentration), (1) 4.0% of neoornidine and 1% of sodium alginate oligosaccharide are diluted by 1000 times; (2) diluting by 1000 times with 1% neoaugustide and 4% sodium alginate oligosaccharide; (3)2.5 percent of neoobinoside and 2.5 percent of sodium alginate oligosaccharide are diluted by 1000 times; (4) 4.0% neooxroside peptide diluted 1000 times; (5) 2.5% sodium alginate oligosaccharide is diluted 1000 times; (6) and (5) clear water control. Repeating the treatment for 3 times, starting the first application at the initial stage of the disease, then spraying for 1 time every 7-10 days, continuously spraying for 3 times, investigating the disease condition of the tobacco mosaic disease on the 10 th day after the third application, calculating the prevention and treatment effect, and obtaining the test result of the field test of the sodium alginate oligosaccharide and neooxonide peptide compound preparation on the tobacco mosaic disease, wherein the test result is shown in 6.
TABLE 6 prevention of tobacco mosaic disease by sodium alginate oligosaccharide and NeoOstrin
Test results show that the compound preparation of the alginate oligosaccharide and the neooxolanyl peptide has a synergistic effect on the prevention effect of tobacco mosaic disease, the prevention effect of the compound treatment of the alginate oligosaccharide and the neooxolanyl peptide is 70.56-74.37 percent, which is higher than the prevention effects of 50.49 percent and 56.87 percent of the compound treatment of the neooxolanyl peptide and the alginate oligosaccharide, and the prevention effect of the compound treatment of 2.5 percent of the alginate oligosaccharide and 2.5 percent of the neooxolanyl peptide is the highest, which is 74.37 percent.
Claims (6)
1. The alginic acid oligosaccharide compound bactericide is characterized by comprising the following components in parts by weight: the active ingredients of the compound agent are alginic acid oligosaccharide and neoaugustine;
wherein the mass ratio of alginic acid oligosaccharide to neoornidazole peptide in the compound agent is 1.0-10: 1.0-10, preferably 2.5: 2.5.
2. The compound bactericide as claimed in claim 1, wherein:
wherein the mass content of the alginic acid oligosaccharide in the compound agent is 1.0-10%, preferably 2.5%; the quality content of neooxroside in the compound agent is 1.0-10%, preferably 2.5%; the balance of water.
3. The compound bactericide as claimed in claim 1 or 2, wherein:
alginate oligosaccharides, also known as Alginate oligosaccharides, have the english name of Alginate oligosaccharides, are oligomers formed by degrading sodium Alginate, and consist of mannuronic acid (M), guluronic acid (G) or hybrid sections of the mannuronic acid and the guluronic acid; the structural formula is shown as formula 1;
neooxonoside peptide, the name of English, Xinnaomycin, has the following structural formula
4. The application of the compound bactericide as claimed in any one of claims 1 to 3, which is characterized in that:
the bactericide can be used for one or more than two of grain crops, cash trees, traditional Chinese medicinal materials, flowers and lawns;
when the diseases occur, one or more than two of the diseases such as rice sheath blight disease, wheat scab, cucumber angular leaf spot, tomato virus disease, pepper virus disease, tomato gray mold, tobacco virus disease and the like can be effectively inhibited and killed.
5. The application of the compound bactericide according to claim 4, which is characterized in that:
the compound bactericide has double functions of protection and treatment, can protect healthy plants from being infected by pathogenic bacteria before disease occurrence, can control disease spread after disease occurrence, has multi-directional sterilization sites, is not easy to generate drug resistance by pathogenic bacteria, can effectively improve plant immunity while preventing and treating diseases, and induces the plants to generate disease resistance. (ii) a
The bacteriostasis rate of the compound bactericide can reach 50-97 percent; the plant yield increasing rate reaches 10-30%.
6. The application of the compound bactericide according to claim 4, which is characterized in that:
the application method of the compound bactericide is one or more than two treatment modes of spraying, seed soaking, root dipping and root irrigation, and is used for treating one or more than two of plant seeds, seedlings or adult plants; diluted 1000 times before administration.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101967182A (en) * | 2009-07-27 | 2011-02-09 | 中国科学院成都生物研究所 | Antimicrobial compound and preparation method and application thereof |
| CN103461340A (en) * | 2013-09-26 | 2013-12-25 | 济源九五生物科技有限公司 | Bactericide composite |
| CN106804594A (en) * | 2015-12-02 | 2017-06-09 | 中国科学院大连化学物理研究所 | A kind of broad spectrum activity oligosaccharides composite bactericide and its application |
| CN108207964A (en) * | 2017-12-12 | 2018-06-29 | 中国科学院成都生物研究所 | A kind of bactericidal composition and its application containing including xin ' ao glycoside peptide and containing amino-oligosaccharide |
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2019
- 2019-11-29 CN CN201911201690.6A patent/CN112868656A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101967182A (en) * | 2009-07-27 | 2011-02-09 | 中国科学院成都生物研究所 | Antimicrobial compound and preparation method and application thereof |
| CN103461340A (en) * | 2013-09-26 | 2013-12-25 | 济源九五生物科技有限公司 | Bactericide composite |
| CN106804594A (en) * | 2015-12-02 | 2017-06-09 | 中国科学院大连化学物理研究所 | A kind of broad spectrum activity oligosaccharides composite bactericide and its application |
| CN108207964A (en) * | 2017-12-12 | 2018-06-29 | 中国科学院成都生物研究所 | A kind of bactericidal composition and its application containing including xin ' ao glycoside peptide and containing amino-oligosaccharide |
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| Title |
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Application publication date: 20210601 |