CN108207964A - A kind of bactericidal composition and its application containing including xin ' ao glycoside peptide and containing amino-oligosaccharide - Google Patents
A kind of bactericidal composition and its application containing including xin ' ao glycoside peptide and containing amino-oligosaccharide Download PDFInfo
- Publication number
- CN108207964A CN108207964A CN201711313658.8A CN201711313658A CN108207964A CN 108207964 A CN108207964 A CN 108207964A CN 201711313658 A CN201711313658 A CN 201711313658A CN 108207964 A CN108207964 A CN 108207964A
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- CN
- China
- Prior art keywords
- amino
- oligosaccharide
- bactericidal composition
- xin
- glycoside peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
Abstract
The present invention relates to a kind of composition pesticide and application thereof, the microbicide compositions and its application of especially a kind of including xin ' ao glycoside peptide using with Antiphytoviral and bacterial activity and the amino-oligosaccharide with induction plant immunizing activities as main active.In the present invention, there is different anti-plant disease mechanism using two kinds of components of including xin ' ao glycoside peptide and amino-oligosaccharide, two kinds of components of including xin ' ao glycoside peptide and amino-oligosaccharide are mixed to the microbicide compositions of compounding, there is apparent synergistic effect in terms of applied to prevention and controlling plant diseases, expand antibiotic and sterilizing range, raising prevents the effect of corps diseases while can reduce the usage amount of chemical pesticide, reduce environmental pollution.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of including xin ' ao glycoside peptide and the bactericidal composition containing amino-oligosaccharide
And its application.
Background technology
Including xin ' ao glycoside peptide is white powder, is soluble in water, dissolves in ethyl alcohol and methanol, molecular weight 445, molecular formula
C16H23N5O10, chemical name is 1- uracil -4- fleshes aminoacyl-serylamino-Isosorbide-5-Nitrae-dideoxy-β-D- glucopyra alditols
Acid, belongs to uracil hexose dipeptides, in silica G F under ultraviolet light254Shown on lamellae yellow-green fluorescence, ninhydrin reaction it is positive,
Biuret reaction is positive, and chemical constitution is:
The antibiotic is from the zymotic fluid of actinomyces " streptomyces noursei " (Streptomyces noursei), through dividing
From, identification obtain.Including xin ' ao glycoside peptide is to the Control of Plant Virus Disease significant effect such as tobacco, capsicum, Tomato yellow mosaic virus, additionally
Bacterial blight of rice, bacterial wilt, soft rot, P. capsici, eggplant cotton disease bacterium, rice blast fungus, small can be prevented
Wheat sheath blight fungus, Sclerotinia sclerotiorum, verticillium dahliae, botrytis cinerea, tobacco black shank bacterium, Colletotrichum capsici,
The plant diseases such as Rhizoctonia solani Kuhn added up experiment and demonstration area nearly 400,000 mu times in recent years, and control effect can reach 60%-
89%.
Amino-oligosaccharide (oligosaccharins) is D- glucosamine units with the low of β-Isosorbide-5-Nitrae glucosides key connection
Glycan, source are mainly obtained by the degradation of natural chitin.Research of the oligosaccharides as the immune activation factor of plant, origin
The sixties in 20th century, by the research and application of over half a century, it is found that amino-oligosaccharide has significant prevention and control farming
The effect of object disease, promote increasing crop yield and improve quality of agricultural product, effect outstanding behaviours can induce crops it is degeneration-resistant,
Disease-resistant crops are induced, promotees crop growth, improve crops quality and promotes increasing crop yield.Oligosaccharide kind biological pesticide at present
It is successfully developed, and is applied in the disease control field of all kinds of crops.
Including xin ' ao glycoside peptide belongs to peptidyl nucleoside antibiotic, and this kind of antibiotic is the important secondary metabolite of microorganism, tool
There is wide spectrum biological activity.It has been found that this kind of antibiosis is known as the specificity for inhibiting protein synthesis, particular by syncaryon
Sugared body peptidyl transferase selectively inhibits amino acid incorporation protein, and it is a variety of that this mode of action provides this kind of antibiotic
Bioactivity.Existing research surface including xin ' ao glycoside peptide is to the Control of Plant Virus Disease effect such as tobacco, capsicum, Tomato yellow mosaic virus
60%-89% can be reached, can additionally prevent the bacteriosises such as bacterial blight of rice, bacterial wilt, soft rot.And amino is few
Sugared element has significant prevention and control corps diseases, promotes increasing crop yield and improves quality of agricultural product effect, is planted by exciting
Gene in object generates chitinase, dextranase, protective plant protecting agent and PR albumen with resistant effect etc., makes plant generation system
Resistance inhibits growth of pathogenic bacteria.
Amino-oligosaccharide described above is mainly by exciting and improving plant immune system, and including xin ' ao glycoside peptide is directly to make
Inhibit the generation of corps diseases for sex pheromone, the mechanism of action of the two is different.If by including xin ' ao glycoside peptide and amino
Oligosaccharide is combined use, in the case where being reduced using including xin ' ao glycoside peptide or being completely inhibited sex pheromone dip dyeing ability, together
When the immune systems of crops itself is excited by amino-oligosaccharide, be expected to obtain effect preferably prevent crop disease viral disease and
The biological pesticide of bacterial disease.
Invention content
For the different mechanism of including xin ' ao glycoside peptide and amino-oligosaccharide on phytopathogen is inhibited, in order to give full play to new Austria
Glycosides peptide passivation plant virus inhibits the effect of plant virus and pathogenic bacteria and amino-oligosaccharide is made full use of to excite farming
The effect of the immune system of object itself expands antibiotic and sterilizing range, improves the effect of prevention corps diseases while can reduce
The usage amount of chemical pesticide reduces environmental pollution, completes the invention of this bactericidal composition.
The specific technical solution of the present invention is as follows:
The effective component of bactericidal composition containing including xin ' ao glycoside peptide and amino-oligosaccharide in the present invention is including xin ' ao glycoside peptide and ammonia
The ratio of weight and number of base oligosaccharide, wherein including xin ' ao glycoside peptide and amino-oligosaccharide is 10:1~1:10.Preferred embodiment is:Including xin ' ao glycoside
The ratio of weight and number of peptide and amino-oligosaccharide is 2:1~1:2.
More than active constituent including xin ' ao glycoside peptide and the 1-70% that the weight ratio of amino-oligosaccharide is the bactericidal composition, preferably
For 6-50%.
Effective component and known auxiliary agent are hybridly prepared into difference during the bactericidal pesticide composition practical application of the present invention
Dosage form, including aqua, soluble powder, soluble granula, water dispersible granules and aqueous emulsion etc..Its dosage form is accounted for effective component
The weight ratio of composition is closely related.
Above-mentioned known auxiliary agent includes:Wetting agent, sticker, dispersant, antifreezing agent, carrier etc..
Wherein, when the bactericidal composition is soluble powder or soluble granula, the auxiliary agent includes wetting agent, glues
Agent, dispersant, surplus is carrier.
Wherein, above-mentioned wetting agent for neopelex, wetting and penetrating agent T, span 20, polysorbate60 etc. one kind or
It is a variety of;The dispersant is alkylsulfonate, alkyl phenol or fatty alcohol ethylene oxide adduct sulfonate, alkyl phenol or fatty alcohol
Phosphate of ethylene oxide adduct etc.;Sticker is anionic surfactant (such as alkylnaphthalene sulfonate, lignin sulfonic acid
Salt), sodium carboxymethylcellulose, polyvinyl alcohol etc.;The antifreezing agent is propylene glycol, glycerine;Carrier is attapulgite, kaolinite
It is one or more in soil, bentonite.
When further bactericidal composition is aqua, the auxiliary agent include the wetting agent of 2-5%, sticker, 1-5% it is anti-
Freeze agent, surplus is water, PH conditioning agents, Preservative qs.
The present invention's contains including xin ' ao glycoside peptide and amino-oligosaccharide microbicide compositions, since two reactive compounds have difference
Mechanism of action can not only directly inhibit plant disease, and the immunocompetence of energy activated plant, improve the anti-of plant for disease
Property, the amount of application of chemical pesticide is reduced, is especially suitable for preventing the viroses of plant such as tobacco, capsicum, Tomato yellow mosaic virus,
The bacteriosises such as bacterial blight of rice, bacterial wilt, soft rot can additionally be prevented.Prove that this is killed through inventor's pre-stage test
Microbial inoculum combination has significant preventive effect.
Specific embodiment
For a better understanding of the present invention, the technology contents of invention are further illustrated below in conjunction with specific embodiment, but
Embodiment of the present invention is not limited to these examples.Due to greenhouse, field trial science in itself put into practice characteristic and experiment
Time, energy, cost are limited, it is impossible to all be verified and be embodied interchangeable technical detail.It should be pointed out that all in ability
Field technique personnel can carry out further screening on the basis of understanding the present invention and equivalent replacement obtains the technology changed,
It should be included within protection scope of the present invention, therefore, the protection domain of patent of the present invention should be determined by the appended claims.
1 50% including xin ' ao glycoside peptide amino-oligosaccharide soluble powder of embodiment
Each component raw material is weighed according to following proportionings:
Including xin ' ao glycoside peptide 30%
Amino-oligosaccharide 20%
Neopelex 3%
Sodium lignin sulfonate 1%
Alkylphenol-polyethenoxy base ether formaldehyde condensate sulfonate 2%
White Carbon black 10%
Attapulgite adds surplus to 100%
By said components mixing, by being crushed to fineness, analytical control, obtaining 50% including xin ' ao glycoside peptide amino-oligosaccharide can
Soluble powder.
2 50% including xin ' ao glycoside peptide amino-oligosaccharide soluble powder of embodiment
Each component raw material is weighed according to following proportionings
Including xin ' ao glycoside peptide 25%
Amino-oligosaccharide 25%
Neopelex 3%
Sodium lignin sulfonate 1%
Alkylphenol-polyethenoxy base ether formaldehyde condensate sulfonate 2%
White Carbon black 10%
Attapulgite adds surplus to 100%
By said components mixing, by being crushed to fineness, analytical control, obtaining 50% including xin ' ao glycoside peptide amino-oligosaccharide can
Soluble powder.
3 6% including xin ' ao glycoside peptide amino-oligosaccharide aqua of embodiment
Each component raw material is weighed according to following proportionings
Including xin ' ao glycoside peptide 3%
Amino-oligosaccharide 3%
Neopelex 3%
Sodium carboxymethylcellulose 2%
Glycerine 4%
Sorbic acid 3%
Water adds surplus to 100%
After above-mentioned each ingredient is dissolved respectively, 6% including xin ' ao glycoside peptide amino-oligosaccharide aqua is made in mixing, analytical control.
4 8% including xin ' ao glycoside peptide amino-oligosaccharide aqua of embodiment
Each component raw material is weighed according to following proportionings
Including xin ' ao glycoside peptide 4%
Amino-oligosaccharide 4%
Wetting and penetrating agent T 3%
Lignosulfonates 2%
Sodium carboxymethylcellulose 2%
Glycerine 4%
Sorbic acid 3%
Water adds surplus to 100%
After above-mentioned each ingredient is dissolved respectively, 8% including xin ' ao glycoside peptide amino-oligosaccharide aqua is made in mixing, analytical control.
5 10% including xin ' ao glycoside peptide amino-oligosaccharide aqua of embodiment
Each component raw material is weighed according to following proportionings
Including xin ' ao glycoside peptide 5%
Amino-oligosaccharide 5%
Neopelex 3%
Span 20 2%
Polyvinyl alcohol 2%
Propylene glycol 4%
Sorbic acid 3%
Water adds surplus to 100%
After above-mentioned each ingredient is dissolved respectively, 10% including xin ' ao glycoside peptide amino-oligosaccharide water is made in mixing, analytical control
Agent.
6 12% including xin ' ao glycoside peptide amino-oligosaccharide aqua of embodiment
Each component raw material is weighed according to following proportionings
Including xin ' ao glycoside peptide 6%
Amino-oligosaccharide 6%
Neopelex 3%
Polysorbate60 2%
Polyvinyl alcohol 2%
Propylene glycol 4%
Sorbic acid 3%
Water adds surplus to 100%
After above-mentioned each ingredient is dissolved respectively, 12% including xin ' ao glycoside peptide amino-oligosaccharide water is made in mixing, analytical control
Agent.
7 6% including xin ' ao glycoside peptide amino-oligosaccharide aqua of embodiment
Each component raw material is weighed according to following proportionings
Including xin ' ao glycoside peptide 4%
Amino-oligosaccharide 2%
Neopelex 3%
Sodium carboxymethylcellulose 2%
Glycerine 4%
Sorbic acid 3%
Water adds surplus to 100%
After above-mentioned each ingredient is dissolved respectively, 6% including xin ' ao glycoside peptide amino-oligosaccharide aqua is made in mixing, analytical control.
8 application test example of embodiment
Application test example is field efficacy Application Example, and the including xin ' ao glycoside peptide amino that above 7 embodiments are prepared is few
Sugared prime system row proportioning pulvis or aqua, carry out the prevention tomato virus disease potting test of pesticide effectiveness.Controlling experiment sets 7 realities altogether respectively
The including xin ' ao glycoside peptide amino-oligosaccharide series for applying example preparation matches pulvis or aqua, 4% including xin ' ao glycoside peptide, 4% amino-oligosaccharide and sky
White control totally 10 processing, often handle 40 plants, and often processing is repeated 3 times.It is tried during Growth of Tomato Seedling to 5-7 piece leaf periods
It tests.Medicament spray and concrete operations it is as follows:
Including xin ' ao glycoside peptide amino-oligosaccharide pulvis or aqua are suitably diluted by dosage, it is equal using miniaturised nebuliser
It is even to be sprayed on Tomato Seedling Leaves.After spraying 3 days, tobacco mosaic virus (TMV) (TMV) is inoculated with, the concrete operations of virus inoculation are such as
Under:
Blade (1g) of the clip fritter containing virus is placed in mortar, and add in suitable 7.2 phosphoric acid buffers of 0.05M pH
Liquid is ground into paste.Filtered through gauze is fallen ill sample juice, and a little diamond dust is spilt on the tender blade face of plant.It is dipped with writing brush few
The sick sample juice of amount gently smears 2-3 times (same direction) on the blade face for spilt diamond dust, cleans blade face with the water in wash bottle later.
It is investigated within 12 days after virus inoculation, investigation result such as table 1.
The processing of 1 different agents of table is to the control effect of tomato virus disease
Claims (7)
1. a kind of bactericidal composition containing including xin ' ao glycoside peptide and amino-oligosaccharide, it is characterized in that:The active ingredient of bactericidal composition is
Including xin ' ao glycoside peptide with Antiphytoviral and bacterial activity and the amino-oligosaccharide with induction plant immunizing activities, wherein new difficult to understand
The mass ratio of glycosides peptide and amino-oligosaccharide is 10:1~1:10.
2. a kind of bactericidal composition containing including xin ' ao glycoside peptide and amino-oligosaccharide according to claim 1 is it is characterized in that new difficult to understand
The preferred embodiment of glycosides peptide and amino-oligosaccharide mass ratio is 2:1~1:2.
3. a kind of bactericidal composition containing including xin ' ao glycoside peptide and amino-oligosaccharide according to claim 1, it is characterised in that living
Property ingredient including xin ' ao glycoside peptide and amino-oligosaccharide weight ratio be the bactericidal composition 1%-70%.
4. a kind of bactericidal composition containing including xin ' ao glycoside peptide and amino-oligosaccharide according to claim 1, it is characterised in that living
Property ingredient including xin ' ao glycoside peptide and amino-oligosaccharide weight ratio preferred embodiment be the bactericidal composition 6%-50%.
A kind of 5. bactericidal composition containing including xin ' ao glycoside peptide and amino-oligosaccharide according to claim 1, it is characterised in that institute
The mass percentage for stating each component of bactericidal composition is active constituent 1%-70%, and surplus is known auxiliary agent.
A kind of 6. bactericidal composition containing including xin ' ao glycoside peptide and amino-oligosaccharide according to claim 1, it is characterised in that institute
It states including xin ' ao glycoside peptide and amino-oligosaccharide and mixes the dosage form for being re-dubbed and allowing on pesticide with known auxiliary agent, including aqua, solubility
Pulvis, soluble granula, water dispersible granules and aqueous emulsion.
7. a kind of application of the bactericidal composition containing including xin ' ao glycoside peptide and amino-oligosaccharide as described in claim 1, feature exist
It can be used for preventing in the bactericidal composition and prevent corps diseases, including tobacco virus, tomato virus disease, capsicum disease
Viral disease, sclerotinia sclerotiorum, rice blast, wheat sharp eyespot, eggplant phytophthora root rot, tobacco black shank.
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Cited By (1)
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CN112868656A (en) * | 2019-11-29 | 2021-06-01 | 中国科学院大连化学物理研究所 | Bactericidal compound agent containing alginate oligosaccharides and neooxonol peptides and application thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103461340A (en) * | 2013-09-26 | 2013-12-25 | 济源九五生物科技有限公司 | Bactericide composite |
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- 2017-12-12 CN CN201711313658.8A patent/CN108207964A/en active Pending
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CN103461340A (en) * | 2013-09-26 | 2013-12-25 | 济源九五生物科技有限公司 | Bactericide composite |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112868656A (en) * | 2019-11-29 | 2021-06-01 | 中国科学院大连化学物理研究所 | Bactericidal compound agent containing alginate oligosaccharides and neooxonol peptides and application thereof |
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Application publication date: 20180629 |