CN112852673A - 一株黑麦草青贮菌株及其应用 - Google Patents
一株黑麦草青贮菌株及其应用 Download PDFInfo
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- 239000004460 silage Substances 0.000 title claims abstract description 44
- 241000209082 Lolium Species 0.000 title claims abstract description 37
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 25
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 25
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 25
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 238000009629 microbiological culture Methods 0.000 claims abstract description 4
- 241000209094 Oryza Species 0.000 claims description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
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- 238000004519 manufacturing process Methods 0.000 abstract description 8
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- 241000894006 Bacteria Species 0.000 description 6
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 6
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- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
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- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
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Abstract
一株黑麦草青贮菌株及其应用，涉及一株青贮菌株及其应用。一株黑麦草青贮菌株，其为植物乳杆菌(Lactobacillus plantarum)1707，保藏在中国微生物菌种保藏管理委员会普通微生物中心，保藏号为CGMCC No.20962。黑麦草青贮菌株在黑麦草青贮中的应用。本发明提供一株黑麦草青贮菌株，该菌株具有产酸多、生长速度快等优点。
Description
技术领域
本发明涉及一株青贮菌株及其应用。
背景技术
黑麦草是一种优质牧草，叶多质嫩，适口性好，可直接喂养草食动物，也可以与精料混合饲喂猪、兔、家禽和鱼等养殖动物。黑麦草青贮可解决供求上出现的季节不平衡和地域不平衡问题，也可解决盛产期雨季不宜调制干草的困难，并获得较青刈玉米品质更为优良的青贮料。发酵良好的青贮黑麦草，具有浓厚的醇甜水果香味，是最佳的冬季饲料。黑麦草营养价值高，富含蛋白质、矿物质和维生素，粗蛋白4.93％，粗脂肪1.06％，无氮浸出物4.57％，钙0.075％，磷0.07％，其中粗蛋白、粗脂肪比本地杂草含量高出3倍。
黑麦草青贮过程一直采用自然青贮或商品通用青贮接种剂，由于青草中乳酸菌菌体丰度低、外源乳酸菌存在适应性弱与竞争性低等问题，黑麦草青贮过程中有时会出现比较大的物料损失甚至失败。
发明内容
本发明提供一株黑麦草青贮菌株，该菌株具有产酸多、生长速度快等优点。
一株黑麦草青贮菌株，其为植物乳杆菌(Lactobacillus plantarum)1707，保藏在中国微生物菌种保藏管理委员会普通微生物中心，保藏号为CGMCC No.20962。
上述黑麦草青贮菌株在黑麦草青贮中的应用。
进一步地，在黑麦草中加入稻糠，再接种植物乳杆菌(Lactobacillus plantarum)1707进行青贮。
青贮过程的基本环境适宜乳酸菌的生长，但前期物料中(特别是表面)会残存空气，因此一些好氧的微生物与乳酸菌一起生长，竞争利用物料中的养分，造成物料腐败损失及后期pH的升高。因此，选用本发明黑麦草青贮菌株可提高青贮饲料中乳酸菌的起始丰度，而且生长速度快，能够加速青贮过程中起始阶段乳酸菌的生长产酸，对青贮有重要意义。
黑麦草中水分含量比较大，直接接种青贮，致使浸出水较多，易造成丁酸菌繁殖产生丁酸，使得青贮品质下降甚至失败，因此本发明加入稻糠以调整物料水分。
本发明植物乳杆菌(Lactobacillus plantarum)1707于2017年7月分离自黑龙江省安达市，黑龙江蓬勃牧草有限公司的黑麦草青贮物料。
植物乳杆菌(Lactobacillus plantarum)1707为植物乳杆菌，属于乳杆菌属(Lactobacillus)；保藏在中国微生物菌种保藏管理委员会普通微生物中心(CGMCC)，保藏地址是北京市朝阳区北辰西路1号院3号，中国科学院微生物研究所，保藏编号为CGMCCNo.20962，保藏日期为2020年10月29日。
具体实施方式
实施例1
4％葡萄糖MRS液体培养基在108℃下灭菌处理15min，接种植物乳杆菌(Lactobacillus plantarum)1707后静止保温28℃培养48hr后检测总酸，总酸采用酸碱滴定法。
植物乳杆菌(Lactobacillus plantarum)1707的产酸量为3.24±0.24％。
实施例2
黑麦草青草在组织捣碎机中加3倍水捣碎，双层纱布过滤，过滤液中补加0.5％葡萄糖，接种植物乳杆菌(Lactobacillus plantarum)1707，静止保温28℃培养，在规定时间检测有效活菌数。
植物乳杆菌(Lactobacillus plantarum)1707培养12hr有效活菌数(0.92±0.17)×108cfu/ml，培养24hr有效活菌数(1.51±0.17)×108cfu/ml，培养36hr有效活菌数(1.49±0.17)×108cfu/ml。
实施例3
黑麦草鲜草切碎至3～5cm，在108℃下灭菌处理15min，冷却后分别接种2.5％的植物乳杆菌(Lactobacillus plantarum)1707菌液和市售青贮接种剂商品(植物乳杆菌)菌液，搅拌混合均匀，分装小袋每袋500g后，抽真空后封口，置28℃下恒温培养一定时间后，检测总酸。
植物乳杆菌(Lactobacillus plantarum)1707培养12hr总酸(以乳酸计％干基)为1.41±0.13％，培养24hr总酸(以乳酸计％干基)为5.50±0.28％，培养36hr总酸(以乳酸计％干基)为5.67±0.13％，培养48hr总酸(以乳酸计％干基)为5.64±0.13％，培养72hr总酸(以乳酸计％干基)为5.66±0.12％。
市售青贮接种剂商品(植物乳杆菌)培养12hr总酸(以乳酸计％干基)为1.00±0.15％，培养24hr总酸(以乳酸计％干基)为4.25±0.13％，培养36hr总酸(以乳酸计％干基)为5.40±0.25％，培养48hr总酸(以乳酸计％干基)为5.50±0.12％，培养72hr总酸(以乳酸计％干基)为5.52±0.20％。
植物乳杆菌(Lactobacillus plantarum)1707产酸在24hr后趋于平稳，而市售青贮接种剂商品(植物乳杆菌)产酸明显滞后，在36hr后趋于平稳，达到峰值后二者差异不显著。表明植物乳杆菌(Lactobacillus plantarum)1707作为黑麦草青贮菌株比市售青贮接种剂商品更具优势。
实施例4
黑麦草鲜草切碎至3～5cm加入10％稻糠，在混合机内接种2.5％的植物乳杆菌(Lactobacillus plantarum)1707菌液和市售青贮接种剂商品(植物乳杆菌)菌液，搅拌均匀，通过打包机塑料膜密封打包，置室内自然青贮1个月，开包检查青贮效果。单生产批次鲜草加工量7吨，鲜草包重量约500公斤。
植物乳杆菌(Lactobacillus plantarum)1707和市售青贮接种剂商品(植物乳杆菌)对黑麦草进行青贮，在1个月青贮饲料的感官指标基本一致，无明显的腐败与大面积霉菌斑点，呈现出正常的青贮物料状态。表1的结果表明，使用本发明植物乳杆菌(Lactobacillus plantarum)1707对黑麦草+稻糠进行打包青贮，与市售青贮接种剂商品(植物乳杆菌)相比在pH、氨肽氮/总氮、中性洗涤纤维、酸性洗涤纤维等四项指标差异显著，呈正向变化；对有机酸乳酸、乙酸、丁酸指标差异不显著，乳酸与乙酸有小幅度提高。
表1
Claims (4)
1.一株黑麦草青贮菌株，其特征在于其为植物乳杆菌(Lactobacillus plantarum)1707，保藏在中国微生物菌种保藏管理委员会普通微生物中心，保藏号为CGMCC No.20962。
2.如权利要求1所述的黑麦草青贮菌株在黑麦草青贮中的应用。
3.根据权利要求2所述的黑麦草青贮菌株在黑麦草青贮中的应用，其特征在于黑麦草中加入稻糠青贮。
4.根据权利要求3所述的黑麦草青贮菌株在黑麦草青贮中的应用，其特征在于稻糠的加入量为黑麦草重量的10±1％。
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| CN102643767A (zh) * | 2012-04-19 | 2012-08-22 | 郑州大学 | 一株植物乳杆菌及其在红薯茎叶发酵和青贮中的应用 |
| CN102851233A (zh) * | 2012-06-12 | 2013-01-02 | 华南农业大学 | 一株耐低温乳酸菌Lactobacillus plantarum CCZZ1及其应用 |
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| CN102643767A (zh) * | 2012-04-19 | 2012-08-22 | 郑州大学 | 一株植物乳杆菌及其在红薯茎叶发酵和青贮中的应用 |
| CN102851233A (zh) * | 2012-06-12 | 2013-01-02 | 华南农业大学 | 一株耐低温乳酸菌Lactobacillus plantarum CCZZ1及其应用 |
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| Title |
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| 巩丽琼等: "多花黑麦草青贮技术研究进展", 《草业与畜牧》 * |
| 王目森: "植物乳杆菌与布氏乳杆菌对多花黑麦草青贮品质及有氧稳定性的影响", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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